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  • Articles  (61)
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  • Articles  (61)
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  • American Association for the Advancement of Science (AAAS)  (61)
  • American Geophysical Union
  • American Meteorological Society
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  • International Union of Crystallography (IUCr)
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  • 1
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    Eye movements of preschool children (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-10-07
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉HD-12572/HD/NICHD NIH HHS/ -- MH-00318/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 1983 Oct 7;222(4619):74-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6623059" target="_blank"〉PubMed〈/a〉
    Keywords: Adult ; Age Factors ; Child, Preschool ; *Eye Movements ; Humans ; Research Design
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Proviral DNA of a retrovirus, human T-cell leukemia virus, in two patients with AIDS (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-05-20
    Description: The acquired immune deficiency syndrome (AIDS) is characterized by T-lymphocyte dysfunction and is frequently accompanied by opportunistic infections and Kaposi's sarcoma. Human T-cell leukemia virus (HTLV) is associated with T-cell malignancies and can transform T lymphocytes in vitro. In an attempt to find evidence of HTLV infection in patients with AIDS, DNA from samples of peripheral blood lymphocytes from 33 AIDS patients was analyzed by Southern blot-hybridization with a radiolabeled cloned HTLV DNA probe. Analysis of DNA from both the fresh (uncultured) lymphocytes and from T cells cultured with T-cell growth factor revealed the presence of integrated HTLV proviral sequences in lymphocytes from two of the patients, both of whom had antibody to HTLV. The proviral sequences could not be detected in blood samples obtained from these individuals at a later date, consistent with the possibility that the population of infected cells had become depleted.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gelmann, E P -- Popovic, M -- Blayney, D -- Masur, H -- Sidhu, G -- Stahl, R E -- Gallo, R C -- New York, N.Y. -- Science. 1983 May 20;220(4599):862-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6601822" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/etiology/immunology/*microbiology ; Adult ; Animals ; Cats ; DNA, Viral/*analysis ; Humans ; Male ; Middle Aged ; *Retroviridae/genetics ; T-Lymphocytes/analysis/microbiology ; Tumor Virus Infections/complications/*microbiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    High efficiency DNA-mediated transformation of primate cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-08-05
    Description: Tissue culture cells from several mammalian species, including three primate lines, were transfected with recombinant vectors carrying Escherichia coli xanthine-guanine phosphoribosyltransferase or Tn5 aminoglycoside phosphotransferase dominant selectable markers. Human HeLa and SV40-transformed xeroderma pigmentosum cells exhibited stable transformation frequencies of at least 10(-3) (0.1 percent). CV-1, an African green monkey kidney cell line, could be stably transformed with the exceptionally high frequency of 6 X 10(-2) (6 percent).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gorman, C -- Padmanabhan, R -- Howard, B H -- New York, N.Y. -- Science. 1983 Aug 5;221(4610):551-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6306768" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Avian Sarcoma Viruses/genetics ; Cell Line ; Cercopithecus aethiops ; Cricetinae ; Cricetulus ; DNA, Recombinant/*metabolism ; Genetic Vectors ; HeLa Cells/metabolism ; Humans ; Mice ; Plasmids ; *Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Modifying oculomotor activity in awake subjects increases the amplitude of eye movements during REM sleep (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-06-03
    Description: The eye movements of human subjects were experimentally modified while they were awake to determine the effect of waking experience on electroculographic activity during rapid eye movement (REM) sleep. After normal eye movements were monitored under controlled conditions, subjects spent 5 days wearing goggles that contained minification lenses and that curtailed vision to a 5 degree field. The amplitude and frequency of eye movements decreased when subjects were awake and increased during REM sleep; sleep stage durations and distributions were unaffected. Values returned to normal in the first 24 hours of recovery.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Herman, J H -- Roffwarg, H P -- MH 3414/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 1983 Jun 3;220(4601):1074-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6844929" target="_blank"〉PubMed〈/a〉
    Keywords: Adult ; Electrooculography ; *Eye Movements ; Humans ; Oculomotor Muscles/physiology ; Sleep, REM/*physiology ; Wakefulness/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    Fragile sites in chromosomes: possible model for the study of spontaneous chromosome breakage (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-04-01
    Description: The tissue culture condition that is required for the type of chromosome breakage seen at most fragile sites, namely, the absence of folic acid and thymidine in the medium, greatly enhanced micronucleus formation in proliferating lymphocyte cultures from normal individuals. This suggests that chromosome breakage at fragile sites and the apparently spontaneous damage that gives rise to micronuclei are controlled by the same mechanism.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jacky, P B -- Beek, B -- Sutherland, G R -- New York, N.Y. -- Science. 1983 Apr 1;220(4592):69-70.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6828880" target="_blank"〉PubMed〈/a〉
    Keywords: Adolescent ; Adult ; Cell Nucleus/drug effects/ultrastructure ; Cells, Cultured ; Child ; *Chromosome Aberrations ; Chromosome Fragile Sites ; *Chromosome Fragility ; Culture Media ; Dose-Response Relationship, Drug ; Female ; Folic Acid/pharmacology ; Humans ; Lymphocytes/ultrastructure ; Male ; Middle Aged ; Thymidine/pharmacology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    Synthesis of kappa light chains by cell lines containing an 8;22 chromosomal translocation derived from a male homosexual with Burkitt's lymphoma (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-12-09
    Description: Three cell lines were derived from a homosexual patient with probable acquired immunodeficiency syndrome and Burkitt's lymphoma. The cell lines produce an unusual strain of Epstein-Barr virus which will both transform cord blood lymphocytes and induce early antigens in Raji cells. Translocations between chromosomes 8 and 22 have occurred in all three lines, but the cells synthesize immunoglobulin M with light chains of the kappa type, in contrast to the usual concordance between a translocation involving chromosome 22 and lambda chain synthesis. Both kappa genes and one lambda gene are rearranged. These findings indicate either that translocation may occur as a separate event from immunoglobulin gene rearrangement or that the proposed hierarchical sequence of immunoglobulin gene rearrangements is not always adhered to. The data also imply that in cells containing a translocation between the long arm of chromosome 8 and a chromosome bearing an immunoglobulin gene, alteration of cellular myc expression may occur regardless of the immunoglobulin gene that is expressed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Magrath, I -- Erikson, J -- Whang-Peng, J -- Sieverts, H -- Armstrong, G -- Benjamin, D -- Triche, T -- Alabaster, O -- Croce, C M -- New York, N.Y. -- Science. 1983 Dec 9;222(4628):1094-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6316501" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/complications ; Antigens, Viral/analysis ; Burkitt Lymphoma/complications/*genetics ; Cell Line ; Chromosomes, Human, 21-22 and Y ; Chromosomes, Human, 6-12 and X ; Epstein-Barr Virus Nuclear Antigens ; Herpesvirus 4, Human/analysis ; Homosexuality ; Humans ; Immunoglobulin Light Chains/*biosynthesis ; Immunoglobulin kappa-Chains/*biosynthesis ; Male ; Oncogenes
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    Potential role of galactokinase in neonatal carbohydrate assimilation (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-04-15
    Description: Glucose given to the newborn human may result in hyperglycemia, suggesting that its utilization is impaired at this developmental stage. Galactose is thought to be a more appropriate carbohydrate source for the newborn. The enzymes involved in hexose phosphorylation may, in part, be responsible for these observations. A key regulatory enzyme of hepatic glucose assimilation, glucokinase, is diminished in newborns compared to adults, whereas galactokinase activity is increased. When newborn dogs were fasted and then fed either glucose or galactose, their plasma insulin responses to glucose were similar, but the pups fed galactose demonstrated an attenuated systemic appearance rate of glucose. Hexose incorporation into hepatic glycogen and net glycogen synthesis was augmented in the galactose-fed dogs. In vitro, liver from neonatal dogs showed enhanced galactokinase activity relative to that for hexokinase or glucokinase. Neonatal hexose assimilation may be independent of insulin action and, instead, be related to the developmental presence of hexose phosphorylating enzymes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kliegman, R M -- Miettinen, E L -- Morton, S -- HD05740/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1983 Apr 15;220(4594):302-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6836273" target="_blank"〉PubMed〈/a〉
    Keywords: Adult ; Animals ; Animals, Newborn/metabolism ; *Carbohydrate Metabolism ; Dogs ; Galactokinase/*physiology ; Galactose/metabolism ; Galactosemias ; Glucose/metabolism ; Humans ; Infant, Newborn ; Liver/enzymology ; Liver Glycogen/biosynthesis ; Phosphorylation ; Rats
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    A silent, neutral substitution detected by reverse-phase high-performance liquid chromatography: hemoglobin Beirut (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-08-26
    Description: A substitution of alanine for valine at position 126 in the beta-chain of hemoglobin was discovered in a hematologically normal adult male of Lebanese extraction. The variant beta-globin was initially observed and subsequently purified by reverse-phase high-performance liquid chromatography (HPLC). Reverse-phase HPLC was also used to isolate the variant tryptic peptide of beta-T13 that has alanine replacing valine at residue 126. The discovery of hemoglobin Beirut illustrates the usefulness of reverse-phase HPLC for the detection of neutral amino acid substitutions in proteins. The ability to detect neutral substitutions in undigested proteins is pertinent to the monitoring of genetic variation in human populations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Strahler, J R -- Rosenbloom, B B -- Hanash, S M -- R01-HL25541/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1983 Aug 26;221(4613):860-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6879181" target="_blank"〉PubMed〈/a〉
    Keywords: Adult ; Chromatography, High Pressure Liquid ; Hemoglobins, Abnormal/genetics/*isolation & purification ; Humans ; Isoelectric Point ; Macromolecular Substances ; Male
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
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  • 9
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    Effects of serotonin on memory impairments produced by ethanol (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-07-29
    Description: Subjects treated with low or high doses of ethanol demonstrated impaired memory, particularly in tests involving the recall of poorly learned information. Zimelidine, an inhibitor of serotonin reuptake, reversed this ethanol-induced impairment. The serotonin neurotransmitter system may mediate learning and memory in humans and may determine some of the effects of alcohol on higher mental functions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Weingartner, H -- Rudorfer, M V -- Buchsbaum, M S -- Linnoila, M -- New York, N.Y. -- Science. 1983 Jul 29;221(4609):472-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6223371" target="_blank"〉PubMed〈/a〉
    Keywords: Adult ; Brompheniramine/analogs & derivatives/pharmacology ; Ethanol/*adverse effects ; Humans ; Learning/drug effects ; Male ; Memory/drug effects ; Memory Disorders/*chemically induced ; Mental Recall/drug effects ; Serotonin/*physiology ; Serotonin Antagonists/pharmacology ; Zimeldine
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
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    DNA methylation decreases in aging but not in immortal cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-06-03
    Description: When normal diploid fibroblasts from mice, hamsters, and humans were grown in culture, the 5-methylcytosine content of their DNA's markedly decreased. The greatest rate of loss of 5-methylcytosine residues was observed in mouse cells, which survived the least number of division. Immortal mouse cell lines had more stable rates of methylation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wilson, V L -- Jones, P A -- 1-T32-CA09320/CA/NCI NIH HHS/ -- R01-GM30892/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1983 Jun 3;220(4601):1055-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6844925" target="_blank"〉PubMed〈/a〉
    Keywords: 5-Methylcytosine ; *Aging ; Animals ; Cell Division ; Cell Line ; Cricetinae ; Cytosine/analogs & derivatives/metabolism ; DNA/metabolism/*physiology ; Fibroblasts/metabolism ; Humans ; Mesocricetus ; Methylation ; Mice ; Time Factors
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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