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  • Springer  (117,788)
  • 1990-1994  (62,697)
  • 1980-1984  (48,999)
  • 1935-1939  (6,092)
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  • 1984  (48,999)
  • 1935  (6,092)
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  • 1990-1994  (62,697)
  • 1980-1984  (48,999)
  • 1935-1939  (6,092)
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  • 1
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    In:  EPIC3Naturwissenschaften, Springer, 71(12), pp. 599-608, ISSN: 0028-1042
    Publication Date: 2014-06-04
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , notRev
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  • 2
    Publication Date: 2020-02-12
    Keywords: 550 - Earth sciences
    Type: info:eu-repo/semantics/book
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  • 3
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 137-144 
    ISSN: 1476-5535
    Keywords: Composting ; Explosives ; Propellants ; Thermophilic ; Mesophilic ; Bioremediation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Composting was investigated as a bioremediation technology for clean-up of sediments contaminated with explosives and propellants. Two field demonstrations were conducted, the first using 2,4,6-trinitrotoluene (TNT), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraazocine (HMX), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and N-methyl-N,2,4,6-tetranitroaniline (tetryl) contaminated sediment, and the second using nitrocellulose (NC) contaminated soil. Tests were conducted in thermophilic and mesophilic aerated static piles. Extractable TNT was reduced from 11840 mg/kg to 3 mg/kg, and NC from 13090 mg/kg to 16 mg/kg under thermophilic conditions. Under mesophilic conditions, TNT was reduced from 11 190 mg/kg to 50 mg/kg. The thermophilic and mesophilic half-lives were 11.9 and 21.9 days for TNT, 17.3 and 30.1 days for RDX, and 22.8 and 42.0 days for HMX, respectively. Known nitroaromatic transformation products increased in concentration over the first several weeks of the test period, but decreased to low concentrations thereafter.
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  • 4
    ISSN: 1476-5535
    Keywords: Fructo-oligosaccharide ; 1-Kestose ; Glycoprotein ; Fructosyl-transferring activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Two extracellular β-fructofuranosidases (E-1 andE-2) fromAureobasidium sp. ATCC 20524, producing 1-kestose (1F-β-fructofuranosyl-sucrose) from sucrose, were purified to homogeneity. Molecular weights of the enzymes were estimated to be about 304000 (E-1) and 315000 (E-2) Da by gel filtration. The enzymes contained 33% (w/w) (E-1) and 27% (w/w) (E-2) carbohydrate. TheK m values for sucrose ofE-1 andE-2 andE-2 were 0.34 and 0.28 M, respectively. were 0.34 and 0.28 M, respectively. The enzymatic profiles of these enzymes were almost identical to intracellular enzymesP-1 andP-2 except for the differences in carbohydrate content andK m values ofE-2 andP-2.
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  • 5
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 149-161 
    ISSN: 1476-5535
    Keywords: Toxin ; Secondary plant metabolite ; Allelochemical ; Insecticide ; Mycotoxin ; Endocytobiont
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Many species of insects cultivate, inoculate, or contain symbiotic fungi. Insects feed on plant materials that contain plant-produced defensive toxins, or are exposed to insecticides or other pesticides when they become economically important pests. Therefore, it is likely that the symbiotic fungi are also exposed to these toxins and may actually contribute to detoxification of these compounds. Fungi associated with bark beetles, ambrosia beetles, termites, leaf-cutting ants, long-horned beetles, wood wasps, and drug store beetles can variously metabolize/detoxify tannins, lignins, terpenes, esters, chlorinated hydrocarbons, and other toxins. The fungi (Attamyces) cultivated by the ants and the yeast (Symbiotaphrina) contained in the cigarette beetle gut appear to have broad-spectrum detoxifying abilities. The present limiting factor for using many of these fungi for large scale detoxification of, for example, contaminated soils or agricultural commodities is their slow growth rate, but conventional strain selection techniques or biotechnological approaches should overcome this problem.
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  • 6
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 163-172 
    ISSN: 1476-5535
    Keywords: Biosensors ; Process control ; Enzyme thermistor ; Immunoassay ; Bio-field effect transistor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A short review about the biosensor research activities for bioprocess monitoring in the F.R.G. after its reunification is given. The principles of biosensor applications are presented. In situ sensors and sensors based on the principles of flow injection analysis are studied. Some applications of a four-channel enzyme thermistor, bio-field effect transistors, and immunoanalysis systems for real process monitoring are presented.
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  • 7
    ISSN: 1476-5535
    Keywords: Vibrio vulnificus ; Oyster ; Monoclonal antibody ; Most probable number ; Enzyme immunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Oysters, suspended particulate matter (SPM), sediment and seawater samples were collected from West Galveston Bay, Texas over a 16-month period and analyzed for the presence ofVibrio vulnificus, a naturally-occurring human marine pathogen. Detection and enumeration ofV. vulnificus was performed using a species-specific monoclonal antibody (mAb FRBT37) in an enzyme immunoassay (EIA)-most probable number (MPN) procedure capable of detecting as few as 2000 target organisms.V. vulnificus was not detected in seawater, oyster or SPM samples during the cold weather months, but was detected at low levels in several sediment samples during this time period. Increased levels of the organism were first observed in early spring in the sediment, and then in SPM and oysters. The major increase inV. vulnificus occurred only after the seawater temperature had increased above 20°C and the winter-spring rainfall had lowered the salinity below 16‰. The highestV. vulnificus levels at each site were associated with suspended particulate matter. These results are consistent with the hypothesis that (1)V. vulnificus over-winters in a floc zone present at the sediment-water interface, (2) is resuspended into the water column in early spring following changes in climatic conditions, (3) colonizes the surfaces of zooplankton which are also blooming during early spring and (4) are ingested by oysters during their normal feeding process.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 235-238 
    ISSN: 1476-5535
    Keywords: Biodegradation ; Pseudomonas putida ; Immobilization ; Sodium cyanide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Pseudomonas putida, isolated from contaminated industrial wastewaters and soil sites, was found to utilize sodium cyanide (NaCN) as a sole source of carbon and nitrogen. Cells, immobilized in calcium alginate beads (1–2 mm diameter) were aerated in air-uplift-type fluidized batch bioreactor containing 100–400 ppm of NaCN. Degradation of NaCN was monitored for 168 h by analyzing gaseous and dissolved ammonia (NH3), CO2, pH and optical density. The results indicated that the alginate-immobilized cells ofP. putida were able to degrade NaCN into NH3 and CO2 in a time-dependent manner.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 229-234 
    ISSN: 1476-5535
    Keywords: Heat shock protein (HSP) ; Yeast ; Saccharomyces ; Viability ; Thermotolerance ; Ethanol tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Heat shock and ethanol stress of brewing yeast strains resulted in the induction of a set of proteins referred to as heat shock proteins (HSPs). At least six strongly induced HSPs were identified in a lager brewing strain and four HSPs in an ale brewing strain. Four of these HSPs with molecular masses of approximately 70, 38, 26 and 23 kDa were also identified in two laboratory strains ofSaccharomyces cerevisiae. The appearance of HSPs correlated with increased survival of strains at elevated temperatures and high concentrations of ethanol. These results suggest that HSPs may play a role in the ethanol and thermotolerance of yeasts. The properties of these proteins and membrane fatty acids in relation to heat and ethanol shock are being investigated.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 239-245 
    ISSN: 1476-5535
    Keywords: Novel polysaccharide ; Bacillus licheniformis ; Raffia venifera ; d-Glucose ; d-Mannose ; d-Xylose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A polysaccharide producing strain ofBacillus licheniformis was isolated from exudate of raffia palm,Raffia vinifera. The optimum conditions for growth and polysaccharide production have been investigated and established. No appreciable polysaccharide was formed on glucose. It grew best in Czapek-Dox media with sucrose as the carbon source. The polysaccharide has been characterized as a heteropolymer containingd-glucose,d-mannose andd-xylose.
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  • 11
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 269-269 
    ISSN: 1476-5535
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  • 12
    ISSN: 1476-5535
    Keywords: β-Fructofuranosidase ; Deglycosylation ; Aureobasidium ; Enzymatic stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Most of the carbohydrate moiety of β-fructofuranosidaseP-1 fromAureobasidium sp. ATCC 20524 was removed by endo-β-N-acetylglucosaminidase F. A subunit of 94000 Da was observed in SDS-PAGE after deglycosylation. TheK m value for sucrose was not changed by deglycosylation but the stability at pH 4–5 and 50°C was decreased. The deglycosylated enzyme was more sensitive to proteases such as pronase E and subtilisin than the native enzyme. It is considered that the carbohydrate moiety of β-fructofuranosidaseP-1 contributes to the stability of the enzyme but is not essential in its catalytic function.
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  • 13
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    Bulletin of mathematical biology 46 (1984), S. 967-969 
    ISSN: 1522-9602
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Mathematics
    Notes: Abstract It is observed that a dynamical continuity equation for biomass distribution yields the asymptotic steady-state exponential dependencen=A exp( $$ - m/\bar m$$ ) exhibited by certain fishery data, wherem is the biomass of an individual,n is the number of individuals per unit biomass interval, andA, $$\bar m$$ are positive constants. This dynamical approach to biomass distribution is an alternative to the global maximization principle proposed recently by Lurié and Wagensberg.
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  • 14
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    Bulletin of mathematical biology 46 (1984), S. 971-972 
    ISSN: 1522-9602
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    Bulletin of mathematical biology 46 (1984), S. 973-974 
    ISSN: 1522-9602
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    Bulletin of mathematical biology 46 (1984), S. 11-17 
    ISSN: 1522-9602
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    Notes: Abstract Linear birth and death processes are used to derive simple expressions for sequential extinction times and gene fixation probabilities in asexual populations.
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  • 17
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    Bulletin of mathematical biology 46 (1984), S. 1-10 
    ISSN: 1522-9602
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    Notes: Abstract We are here concerned with the functionf which assigns to each pointP of an object the numberf(P) which is the shortest distance fromP to the border. This function appears in various guises in diverse biological studies. The functionf(P) is itself a measure of shape—or more precisely, an infinite set of measures, one for each point (and hence, in view of its geometric definition, usually in a form inconvenient for use). Thus in this paper we sought a reasonable representative of this infinite set of measures, namely themean of the numbersf(P) asP ranges over all points of the entity. Computability studies are developed for various classes of shapes. For example, (1) the mean for a lamina bounded by a polygon circumscribable about a circle of radiusr isr/3; (2) the mean for a domain bounded by a polyhedron circumscribable about a sphere of radiusr isr/4. The transition from pointwise to piecewisef(P), especially in the non-convex case, requires working with inequalities.
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    Bulletin of mathematical biology 46 (1984), S. 19-40 
    ISSN: 1522-9602
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    Notes: Abstract A mathematical model for traveling bands of motile and chemotactic bacteria in the presence of cell growth and death is examined. It is found that asymptotic traveling wave solutions exist in the absence of chemotaxis, due to the balance of growth, death and random motility. Thus random motility confers the ecological advantage of population propagation through migration into nutrient-rich regions. The presence of chemotaxis amplifies this advantage by moving more cells into higher nutrient concentration regions, resulting in larger and faster bands. Therefore there seem to be two types of traveling bands that can be attained by chemotactic bacteria in the presence of growth and death: (1) these growth/death/motility bands; and (2) pure chemotactic ‘Keller-Segel'-type bands. Comparison to experimental observations by Chapman in 1973 indicate that the latter seem to be formed. The relationship between these two types of solution is at present uncertain. The growth/death/motility bands may have relevance on longer time or distance scales characteristic of microbial ecological systems.
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    Bulletin of mathematical biology 46 (1984), S. 115-125 
    ISSN: 1522-9602
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    Notes: Abstract Based on the principle of minimum power, a mathematical model of the pathological functional state of the oxygen transport system is presented. The model is used to determine the optimal functional parameters of the oxygen transport system in hyperthyroidism, anemia and hypertension. Theoretical results are compared with clinical data.
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    Bulletin of mathematical biology 46 (1984), S. 139-153 
    ISSN: 1522-9602
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    Notes: Abstract A modified SIRS model is developed as a framework for the study of epizootiological dynamics in an insect-pathogen system. Linearized stability analysis reveals that the system with one immune and one susceptible host class can exhibit stable, periodic or unstable behavior depending on model parameters. In general, high pathogenicity, short pathogen propagule lifespan and high host reproductive rate are stabilizing influences. Pathogen transmissibility and propagule production/host do not influence local stability. The effect of seasonal host reproduction is studied because most insect hosts are seasonal in temperate climates. The basic stability dependence on model parameters holds except as modified by the length of the reproduction interval. The results of this study are compared with the recent work of Anderson and May.
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    Bulletin of mathematical biology 46 (1984), S. 175-184 
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    Notes: Abstract The random predator-prey type interactions of the population species in a random varying environment have been investigated. The Fokker-Planck equation for the transition probability, corresponding to the stochastic differential equations established from Lotka-Volterra equations by the introduction of randomness and variability, has been integrated in the form of a path integral. The transition probabilities for extinction or survival of one or several species have been approximately evaluated and investigated.
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    Bulletin of mathematical biology 46 (1984), S. 155-174 
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    Notes: Abstract If two microbial populations compete for a single resource in a homogeneous environment with time invariant inputs they cannot coexist indefinitely if the resource competed for is not renewed by biological activity within the system. Mathematical studies have shown that in a predator-prey system, where the resource (prey) is self-renewing, the two competitors (predators) can coexist in a limit cycle. This suggests that if the resource competed for is renewed by biological activity within the system coexistence can occur in any microbial system provided that it exhibits the same features as, but without being, a predator-prey one. A food chain involving commensalism, competition and amensalism is presented here. Two subcases are considered. It is only when maintenance effects are taken into account that coexistence, in limit cycles, can occur for this system. Limit cycle solutions for the system are demonstrated with the help of computer simulations. Some necessary conditions for coexistence are presented, as are some speculations regarding the possible physical explanations of the results.
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    Bulletin of mathematical biology 46 (1984), S. 127-137 
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    Notes: Abstract The branching structure of the mammalian arterial tree has been known to be close to that of an optimal conduit system of the minimum work model characterized as the branch system of constant wall shear rate. The physiological mechanism producing such construction was considered to be based on the local response of arterial caliber induced by the wall shear stress (shear rate × blood viscosity) and thereby maintaining this stress constant, which was previously observed at the canine common carotid artery shunted to the external jugular vein. The stress levels at various parts of the arterial system estimated from available data fell within ±50% of the mean (15 dyn/cm2), which was consistent with the value predicted from the model. Theoretical analyses on the cost function of the model indicated that the suspected variation of shear rate levels in the arterial tree due to the anomalous changes in blood viscosity which might bring about 3- to 4-fold differences between the minimum and maximum shear rates would cause less than 10% increase in the total energy cost. It was concluded that a local adaptive response to wall shear stress is the mechanism which effectively optimizes the design of the arterial tree.
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    Bulletin of mathematical biology 46 (1984), S. 185-185 
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    Bulletin of mathematical biology 46 (1984), S. 187-203 
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    Notes: Abstract The structure of the genetic code is related to a Gray code, which is a plausible theoretical model for an amino acid code. The proposed model implies that the most important factor in shaping the code was the effects of mistakes in translation, not effects of mutations. Another possible implication is that the preservation of stiffness and flexibility at appropriate places in a protein chain is as important in protein structure as the appropriate placement of hydrophilic (external) and hydrophobic (internal) residues. Other results are a simple conceptualization of the relationships among the 20 amino acids and their relations to their codons. The detailed relationships are summarized in the following ‘similarity alphabet’: ala, thr, gly, pro, ser; asp, asn, glu, gln, lys; his, arg, trp, tyr, phe; leu, met, ile, val, cys; (ATGPS DNEQK HRWYF LMIVC in the one-letter code). This alphabet falls into four groups of amino acids: small, external, large, internal. The approximate relation of the groups to their codons is expressed as: the first base of a codon controls size—a purine means a small amino acid, a pyrimidine means large; the middle base controls cloisterednes—purine means external, pyrimidine means internal. These relationships express the minimum change principle upon which the code appears to be founded.
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    Bulletin of mathematical biology 54 (1992), S. 1-20 
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    Notes: Abstract An organism persists only if it satisfies internal and external constraints. Within the organism networks of processes meet the constraints. In such networks a principle of matching often obtains: the pattern of coupling among processes matches the correlation among constraints. That is, a module—a cluster of coupled processes—meets a constraint. Dissociable modules meet dissociàble constraints. A hierarchy of modules meets a hierarchy of constraints. We have inquired whether such matching is predicted by an optimality criterion in a simple example. We find that in an ensemble of networks with unreliable processes, the networks that meet the constraints with highest reliability obey the principle of matching. The difference in reliability between modular and nonmodular networks that meet the same constraints is a function of the probability of success per process. Our results suggest that this difference is maximal at a probability of success that increases monotonically with the number of processes in the network.
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    Bulletin of mathematical biology 54 (1992), S. 59-75 
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    Notes: Abstract A method is described for estimating the distribution and hence testing the statistical significance of sequence similarity scores obtained during a data-bank search. Maximum-likelihood is used to fit a model to the scores, avoiding any costly simulation of random sequences. The method is applied in detail to the Smith-Waterman algorithm when gaps are allowed, and is shown to give results very similar to those obtained by simulation.
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    Bulletin of mathematical biology 46 (1984), S. 269-282 
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    Notes: Abstract A theoretical study of the Brusselator model with non-uniform distribution of component A and a concentration-dependent diffusion coefficient has been performed. Numerical simulation reveals that a variable diffusion coefficient alters the bifurcation pattern and the stability properties of the steady-state as well as periodic solutions. A simple approximate method, based on one-point collocation, has been proposed to analyze the bifurcation phenomena for the case of fixed boundary conditions and low system size.
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    Bulletin of mathematical biology 46 (1984), S. 283-294 
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    Notes: Abstract In this paper the effects of the occurrence of cut trees in the topological analysis of branching patterns have been studied. It is assumed that branches are removed at random from the trees. We prove that, for both the segmental and terminal growth models, the probability distributions of the cut trees are identical to those of complete trees.
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    Bulletin of mathematical biology 46 (1984), S. 247-268 
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    Notes: Abstract The goal of this work is an examination of capillary exchange models as mathematical operators. The concentration function relations for the Krogh cylinder of a single capillary, basic to many organ models, are studied via the theory of operators on the Lebesgue normed spacesL p[0,∞], (1〈-p〈-∞). A discussion is included of theL p -normsvis-à-vis the coefficient of variation currently used in finding capillary parameters and evaluating parameter searches. The capillary model determines two operators on the space of locally integrable functions: O K (relating extravascular concentration to intravascular) and K a, k (relating intravascular concentration to input), wherek is the ratio of permeabilitysurface area (PS) to extravascular volume, and α is the ratio of PS to flow. These operators are shown to induce contractive (‖O K ‖ p 〈-1, ‖K a, k ‖ p 〈-1), isotone, linear operators onL p . The uniform convergence relation $$K_{a,k} = \mathop {\lim _{(p)} }\limits_{N \to \infty } \left( {\sum\limits_{n = 0}^N {P_n (a)O_k^n } } \right)$$ (as operators onL p) is derived, whereP n (a) is the Poisson probabilitye −a a n /n!. For the important special cases ofp=∞, 1, 2 the norms are found (‖Ok‖=‖Ka,k‖p=1). Consideration is also given to the norms and operators when the functions involved are limited to a finite interval of time.
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    Bulletin of mathematical biology 46 (1984), S. 295-326 
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    Notes: Abstract One particular kind of structure offers possible explanations, for long-term memory, efficient consolidation of stored information from the environment, clustering of data strings and multimodal functioning. It is a possible model for pieces of neural structure and its use offers a uniform method for both studying and constructing an extensive class of mechanisms.
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    Bulletin of mathematical biology 46 (1984), S. 327-332 
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    Notes: Abstract Levenshtein dissimilarity measures are used to compare sequences in application areas including coding theory, computer science and macromolecular biology. In general, they measure sequence dissimilarity by the length of a shortest weighted sequence of insertions, deletions and substitutions required, to transform one sequence into another. Those Levenshtein dissimilarity measures based on insertions and deletions are analyzed by a model involving valuations on a partially ordered set. The model reveals structural relationships among poset, valuation and dissimilarity measure. As a consequence, certain Levenshtein dissimilarity measures are shown to be metrics characterized by betweenness properties and computable in terms of well-known measures of sequence similarity.
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    Bulletin of mathematical biology 46 (1984), S. 337-337 
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    Bulletin of mathematical biology 46 (1984), S. 333-336 
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    Notes: Abstract It is pointed out that the methane flux measured experimentally for certain ponds and swamps is quantitatively consistent with a commensal dependence of Methanobacteria on O2-chemotactic motile aerobic bacteria. The Methano species is thereby shielded from oxygen and provided with carbon dioxide for the anaerobic production of methane.
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    Bulletin of mathematical biology 46 (1984), S. 357-370 
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    Notes: Abstract A sufficient condition is given for stochastic boundedness persistence of a top predator in generalized Lotka-Volterra-type stochastic food web models in arbitrary bounded regions of state space. The main result indicates that persistence in the corresponding deterministic system is preserved in the stochastic system if the intensities of the random fluctuations are not too large.
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    Bulletin of mathematical biology 46 (1984), S. 371-377 
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    Notes: Abstract One of Bobisud's models for the evolution of cannibalism is reanalyzed by applying the method of finding evolutionarily stable strategies (or ESS's). It is demonstrated that ‘no cannibalism’ never will be an ESS if the initial rate of cannibalism is too large. It is further demonstrated that individual selection may even result in the evolution of cannibalism during food abundance. Some empirical case studies are briefly discussed in relation to this model.
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    Bulletin of mathematical biology 46 (1984), S. 379-387 
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    Notes: Abstract A computer algorithm is presented which equiprobably generates any member of the set of all directed trees withk labeled terminal nodes and unlabeled interior nodes. The algorithm requires roughlyk 2 /2 storage locations. The one-time initialization requiresO(k 2 ) time, while generating each tree requiresO(k) time.
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    Bulletin of mathematical biology 54 (1992), S. 401-412 
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    Notes: Abstract We develop a theory for host seeking decisions in mosquitoes that explicitly considers the tradeoffs mosquitoes face in allocation to somatic and gametic function. Specifically, we consider conditions under which mosquitoes should seek out nectar and blood hosts upon encountering host odours. Results from development of a dynamic model that considers free and crop energy states suggest that mosquitoes should seek out blood hosts under a wide variety of conditions but that decisions to seek nectar depends upon crop volume, concentration and free energy. This pattern arises because mosquitoes carrying large crop loads are constrained in their ability to obtain large blood meals due to space limitations in the abdomen. The predicted patterns of behaviour are supported by published observations of mosquito behaviour.
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    Bulletin of mathematical biology 54 (1992), S. 413-422 
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    Notes: Abstract In behavioral ecology, diet selection is often predicted by using a theory in which it is assumed that behaviors have evolved to maximize the rate of intake of energy. In this theory, it is assumed that fitness is a monotonic function of energy intake. An alternative is to deal directly with fitness, measured in terms of expected reproduction, and thus connect short term behavior with reproduction. Following the usual assumptions of the theory of diet choice, fitness satisfies a partial differential-difference equation. Conditions under which rate maximizing and state variable theories of diet selection yield identical predictions are identified. When predation, as well as starvation, is a source of mortality, the identification is not as complete.
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    Bulletin of mathematical biology 54 (1992), S. 445-464 
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    Notes: Abstract Environmental uncertainty can be both a cause and consequence of chance variation in many of the phenotypic factors associated with the control of clutch size in birds. When such uncertainty inflates or otherwise influences the variance associated with expected reproductive success for any genotype, it will also influence the resulting phenotypic optima. Random variation that affects the evolution of clutch size optima explicitly may occur both within (intra-) and across (inter-) generations. Examples of intra-generational uncertainty could include chance variation in: (1) the quality and quantity of offspring, (2) parental quality, and (3) temporal resources like food. Inter-generational uncertainty would include chance variation in demographic and population characters. With respect to clutch (or litter) size, almost all forms of uncertainty tend to favor an optimum (genetic) strategy with a clutch that is smaller than the clutch associated with the apparent or actual maximal fitness of an individual parent. The overall effect of all the components of uncertainty can be evaluated through the integration of all this phenotypic variation: however each step of the integration is a conditional expectation of each component. Therefore, a single factor analysis may indicate a false optimum, and an integrated analysis of all components is necessary to evaluate the importance of their individual and joint effects on the adaptive evolution of clutch size.
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    Bulletin of mathematical biology 54 (1992), S. 503-519 
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    Notes: Abstract A difference equation model for the dynamics of a semelparous size-structured species consisting of juvenile and adult individuals is derived and studied. The adult population consists of two size classes, a smaller class and a larger more fertile class. Negative feedback occurs through slowed juvenile growth due to increased total population levels during the developmental period and consequently a smaller adult size at maturation. Intra-specific competition coefficients are size dependent and measure the strength of intra-specific competition between juveniles and adults. It is shown that equilibrium states in which adults and juveniles occur together at all times are in general destabilized by significantly increased juvenilevs adults competition with the result that stable periodic cycles appear, in which the generations alternate in time and hence avoid competition. This result supports the tenet that intra-specific competition between juveniles and adults is destabilizing. Exceptions to this destabilization principle are found, however, in which populations exhibiting non-equilibrium, aperiodic dynamics can be equilibrated by increase competition between juveniles and adults. This occurs, for example, when adult fertility and competition coefficients are significantly size class dependent.
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    Bulletin of mathematical biology 54 (1992), S. 537-561 
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    Notes: Abstract An epithelial cell is modeled as a single compartment, bounded by apical and basolateral cell membranes, and containing two nonelectrolyte solute species, nominally NaCl and KCl. Membrane transport of these species may be metabolically driven, or it may follow the transmembrane concentration gradients, either singly (a channel) or jointly (a cotransporter). To represent the effect of stretch-activated channels or shrinkage-activated cotransporters, the membrane permeabilities and cotransport coefficients are permitted to be functions of cell volume. When this epithelium is considered as a dynamical system, conditions are indicated which guarantee the uniqueness and stability of equilibria. Experimentally, many epithelial cells can regulate their volume, and such volume regulatory capability is defined for this model. It is clearly distinct from dynamical stability of the equilibrium and requires more stringent conditions on the volume-dependent permeabilities and cotransporters. For a previously developed model of the toad urinary bladder (Strieteret al., 1990,J. gen. Physiol. 96, 319–344) the uniqueness and stability of its equilibria are indicated. The analysis also demonstrates that under some conditions a second stable equilibrium may appear, along with a saddle-node bifurcation. This is illustrated numerically in a modified model of the epithelium of the thick ascending limb of Henle.
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    Bulletin of mathematical biology 54 (1992), S. 687-694 
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    Bulletin of mathematical biology 54 (1992), S. 649-672 
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    Notes: Abstract Models of the dynamical interactions important in generating immune reactivity have generally assumed that the immune system is a single well-stirred compartment. Here we explicitly take into account the compartmentalized nature of the immune system and show that qualitative conclusions, such as the stability of the immune steady state, depend on architectural details. We examine a simple model idiotypic network involving only two types of B cells and antibody molecules. We show, for model parameters used by De Boeret al. (1990,Chem. Eng. Sci. 45, 2375–2382), that the immune steady state is unstable in a one compartmental model but stable in a two compartment model that contains both a lymphoid organ, such as the spleen, and the circulatory system.
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    Bulletin of mathematical biology 46 (1984), S. 515-527 
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    Notes: Abstract The comparison of several sequences is central to many problems of molecular biology. Finding consensus patterns that define genetic control regions or that determine structural or functional themes are examples of these problems. Previously proposed methods, such as dynamic programming, are not adequate for solving problems of realistic size. This paper gives a new and practical solution for finding unknown patterns that occur imperfectly above a preset frequency. Algorithms for finding the patterns are given as well as estimates of statistical significance.
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    Bulletin of mathematical biology 46 (1984), S. 501-514 
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    Notes: Abstract A new development is introduced here in the use of dynamic programming in finding pattern similarities in genetic sequences, as was first done by Needleman and Wunsch (1969). A condition of pattern similarity is defined and an algorithm is given which scans any set of similarities and screens out those which fail to meet the condition. When the set to be scanned contains every pair of segments, one from each of two given sequences of lengthsm andn (i.e. every possible location for a pattern similarity), then it completes the scan in a number of computational steps proportional tom·n, leaving those pairs of segments which satisfy the similarity condition. The algorithm is based on the concept of match density, as suggested by Goad and Kanehisa (1982).
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    Bulletin of mathematical biology 46 (1984), S. 529-543 
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    Notes: Abstract This paper concerns sequences of letters in which certain “distinguished” words are of interest. Such sequences arise as data in numerous fields including genetics and neuroscience. A probability distribution is given for the number of occurrences of a chosen word in a randomized sequence of letters. Such words are considered “favored” if they occur more than expected at random. Favored words have been discovered in nerve impulse trains and may reflect a neural coding scheme.
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    Bulletin of mathematical biology 46 (1984), S. 545-552 
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    Notes: Abstract As the volume of protein sequence data grows, rapid methods for searching the protein sequence database become of primary importance. Rigorous comparison of sequences is obtained with the well-known dynamic programming algorithms. However, these algorithms are not rapid enough to use for routinely searching the entire database. In this paper we discuss some methods that can be used for rapid searches.
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    Bulletin of mathematical biology 46 (1984), S. 553-566 
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    Notes: Abstract We give algorithms for computing the extent of similarity between two or three sequences of letters. The similarity measures we consider include a penalty for inserting gaps within the sequence in order to enhance similarity. The magnitude of the penalty for gaps is assumed to be independent of their size in order to accommodate certain biological applications. Our algorithm for three sequence comparisons, which is based on solving a system of recursive equations, improves upon the efficiency of existing methods. Although the system of recursive equations utilized by the algorithm is quite complicated as it stands, it has none the less been simplified by appeal to combinatorial considerations.
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    Bulletin of mathematical biology 46 (1984), S. 567-577 
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    Notes: Abstract Well-known dynamic programming algorithms exist for comparing two finite sequences inO(N 2) time and storage, whereN is the common sequence length. Extensions to the comparison ofM finite sequences requireO((2N) M) time and storage, making such algorithms difficult even forM=3. A simple generalization of the sequences makes it possible to obtain some results about the geometry of sequence alignments. These ideas suggest heuristic approaches to problems of comparing several sequences. IfM sequences are known to be related by a binary tree, they can be aligned inO(MN 2) time andO(N 2+NM) storage.
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    Bulletin of mathematical biology 54 (1992), S. 839-873 
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    Notes: Abstract Using the chromium release assay and the single cell assay in agarose, we study the cytotoxic reaction of the MHC-restricted T lymphocyte clones P89:15 and P1:3, which recognize distinct but specific tumour antigens on the surface of syngeneic P815 mastocytoma cells. We propose a mathematical model which describes these experiments, accounts for the strongly non-Michaelian behaviour of the reaction and permits us to estimate the kinetic parameters characterizing effector-target conjugation and lethal hit delivery. The results show that the binding and lytic activity of effector cells is modulated by the number of targets bound to them. The binding of a second target by an effector having already a target bound is facilitated; on the other hand, an effector having bound two targets delivers a lethal hit more slowly than one with a single target bound. We investigate the role of these kinetic properties in the competition between the process of tumour progression due to cancer cell replication and the process of tumour regression due to T lymphocyte cytotoxic activity. For both clones, we estimate the effector-target ratio beyond which rejection prevails. This ratio is nine times larger for P1:3 than for P89:15. Furthermore, our analysis suggests that there exists an optimal specificity minimizing this ratio. Deviations from this optimum, be it in the sense of an increase or decrease of specificity, tendsto stabilize the tumoural state: a situation which in the broader context of the immune response evolution and regulation can be viewed as animmune response dilemma.
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    Bulletin of mathematical biology 54 (1992), S. 929-938 
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    Notes: Abstract In this paper a stochastic model for a two-compartment system which incorporates Erlang residence time distributions (i.e. the residence times have the gamma distribution where the shape parameters assume integer values only) into each compartment is generalized to include random rate coefficients. Analytical forms of the model are derived for the case where the rate coefficients have gamma densities. A relationship is established between the new models and existing models that are in current practical usage.
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    Bulletin of mathematical biology 54 (1992), S. 939-955 
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    Notes: Abstract Oscillations and chaos can be modelled and observed in a realistic simulation model of interacting prey-predator populations based on Monte Carlo simulation methods. These nonlinear phenomena are linked with some biological and physical bifurcation parameters and mathematical tools from dynamical systems theory may be used in order to characterize this behaviour. Chaotic dynamics are therefore, in our simulation, more the rule than the exception, and are related to delays associated with spatial degrees of freedom.
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    Bulletin of mathematical biology 46 (1984), S. 661-672 
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    Notes: Abstract Protein sequences of the Dayhoff databank of 1984 have been analyzed to evaluate the occurrences of the 400 dipeptides and 8000 tripeptides. Expected values and standard deviations for the di- and tripeptides were determined by Monte Carlo and binomial approximation. A condensed format containing this information, labeled a uniqueness diagram, is presented and made available in the form of a microfiche.
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    Bulletin of mathematical biology 54 (1992), S. 1023-1038 
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    Notes: Abstract Cells displaying the classic multidrug resistant (MDR) phenotype possess a transmembrane protein (p170 or P-glycoprotein) which can actively extrude cytotoxic agents from the cytoplasm. A mathematical model of this drug efflux pump has been developed. Outward transport is modeled as a facilitated diffusion process. Since energy-dependent efflux of cytotoxic agents requires that ATP also bind to p170, the model includes a dynamic calculation for efflux rate which considers Michaelis-Menten kinetics for both the substrate agent and ATP. The final system consists of one partial differential equation (PDE) for the facilitated diffusion of substrate agents out of the cell a 2×2 ordinary differential equation (ODE) system for the dynamic calculation of the ATP-ADP pool, and a dynamic algebraic calculation of the efflux rate given substrate levels at the interior cell membrane interface and ATP levels in the cell. A stability analysis of the ATP-ADP pool distribution and a simplistic closed form solution of the linearized PDE are included. Numerical simulations are also provided.
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    Bulletin of mathematical biology 54 (1992), S. 1039-1056 
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    Notes: Abstract Reentry in the heart is the repeated excitation of the same tissue by a single excitation wave; it is responsible for several types of cardiac arrhythmia. The simplest model which permits the phenomenon of reentry is two laterally coupled excitable fibers; in this paper we examine such a model in order to establish a basis for the understanding of the fundamental physical processes underlying the process of reentry. Two versions of the FitzHugh-Nagumo equations are used to develop complementary numerical and analytical results for the coupled fiber model. On the basis of numerical studies, regions of qualitatively different behaviour are mapped in the parameter space of excitation threshold and coupling strength between the fibers, and the effect of the rate of recovery is explored. Some of these regions are also obtained analytically, in good agreement with the numerical results. Finally, the results are discussed in the light of recent work on the role of the anisotropy of cardiac tissue in the initiation of reentrant activity in the heart.
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    Bulletin of mathematical biology 54 (1992), S. 1069-1081 
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    Notes: Abstract A sigmoid curve with three fitting parameters is proposed as a descriptive model for the spatial velocity field in one-dimensional growth of plant organs. Analytic expressions are derived for the relative elemental growth (REG) rate, the position and value of the REG rate maximum, the length of the growth zone, the inverse of the growth trajectory and cell length in the “elongation only” zone. The expressions are fit to published data to characterize the effects of environmental variation on growth of monocotyledonous roots. The simple expressions for growth may prove useful in mechanistic models. The fitted curves summarize more than a decade of observations and thus provide a challenge to theorists.
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    Bulletin of mathematical biology 46 (1984), S. 827-844 
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    Notes: Abstract In two-state sliding filament models of muscle contraction a partial differential equation must be solved to find the cross-bridge distribution functionn(x, t). In this paper the analytical form of this function is obtained by integration along the characteristic line and special cases are presented in which the explicit expression forn(x, t) can be completely determined. These analytical solutions provide a direct mathematical connection between the microscopic contraction parameters contained in the kinetic theories and macroscopic muscle dynamics and are thus used to investigate what parameters influence the transient contractile tension in typical experimental conditions. The results of this investigation are consistent with relevant aspects of muscle physiology.
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    Bulletin of mathematical biology 46 (1984), S. 845-857 
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    Notes: Abstract Experiments on random binary, ternary, etc. (P=2, 3,…, 10) switching nets are reported. Behavioral cycle lengths are examined as functions of output variety,P, input connectance,K, and net size,N. Overall, output variety appears an influential, well-behaved net property. Strong, but well-behaved interactions appear among net variables. In high connectance nets, median cycle length grows approx. asP N/2. Other factors constant, one-connected nets show the shortest cycles, and connectance effects appear to converge asymptotically aroundN. Data for cycle length as a function of net size suggest a concavity not compatible with the Kauffman “square root law” (Kauffman, 1969). Evidence of a positive relationship between cycle length and run-in length is found in two-input nets; weaker evidence is obtained that in higher connectance nets this relationship becomes negative in sign. The “modular complexity” ofP〉2 nets is examined briefly.
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    Bulletin of mathematical biology 46 (1984), S. 869-877 
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    Notes: Abstract The influence of topographical situation on the spread of infection is studied. The investigation is based on a multigroup model. The population under consideration is thought to be divided into subpopulations living in regions that are separated from each other by natural barriers (mountains). Infection is carried from one region to another by migrating infectives. Migration is possible only along the river system so that the structure of the epidemiological network is that of a symmetric tree. The results allow comparison of the velocity of propagation of the epidemic for different geographical situations and allow quantification of the “channel-effect”, according to which mountainous regions are channels rather than barriers to the spread of an epidemic.
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    Circuits, systems and signal processing 11 (1992), S. 115-136 
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    Notes: Abstract By considering all orderings of the input samples, which are discrete-time continuous-valued, it is shown here that a weighted median (WM) filter of spanN can be specified unambiguously by 2N−1 consistent linear inequalities relating the weights. This specification is identical to that of a self-dual threshold function with the same weights. It is also shown that WM filters with symmetric weights can be specified by ternary threshold functions. Based on these inequalities, properties of WM filters which can be used to check equivalence of some WM filters are derived.
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    Circuits, systems and signal processing 11 (1992), S. 137-151 
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    Notes: Abstract Because binary mathematical morphology permits fast local neighborhood operations by flash conversion, it is used extensively in high-speed pattern-recognition computer systems. Further, since anyN-dimensional integer function may be represented by an (N + 1)-dimensional binary (bilevel) function, ordinary two-dimensional graylevel images become three-dimensional binary images. Thus these images may be processed by high-speed flash-conversion computers assuming that a sufficiently compact three-dimensional kernel can be devised. The tetradekahedron of the face-centered-cubic tessellation forms a perfect kernel in three-dimensions. Its neighborhood is compact. It has total symmetry with all 12 neighbors equidistant from the central element. Using this kernel a variety of useful three-dimensional morphological operations may be performed for target track detection, shaded graphics, data clustering, automated focusing, and spatial filtering.
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    Circuits, systems and signal processing 11 (1992), S. 153-169 
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    Notes: Abstract Two techniques for image restoration are compared in this paper. One is a technique based on the theory of optimal adaptive stack filtering; the other is a recently developed vector detection approach to image restoration. The primary difference between these two techniques is that the optimal detection technique exploits multilevela priori information, while the stack filter uses only single level zero crossing information. The design constraints for stack filters and vector detection are similar. Both approaches rely on the existence of a training sequence for the image source in order to obtain optimal processing. Adaptive stack filters do, however, require a training set of the noise while the optimal detection approach only needs a multivariate parametric representation. The image-restoration performance of these two methods is compared in a signal dependent noise environment characterizing imaging systems with speckle, film-grain, and Poisson shot noise. Comparisons are made using the mean absolute error measure as well as a subjective measure.
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    Circuits, systems and signal processing 11 (1992), S. 325-352 
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    Notes: Abstract The stability of digital ladder filters close in form, via the lossless discrete integrator (LDI) transformation, to doubly terminatedLC Cauer ladder low-pass filters is studied. The LDI transformation does not necessarily map stable analogue into stable digital filters. Necessary and sufficient conditions for these digital filter to be lossless and for a corresponding doubly terminated filter to be stable are given. LDIs are often used in the design of switched capacitor (SC) filters. For this case we provide a threshold sampling rate above which the SC LDI filter retains the stability of the analogue filter. In spite of the stability problem with the LDI mapping, it has been observed that when applied to simulate analogue filters the resulting filter is stable. It can now be argued that, in these cases, other factors in the determination of the sampling rate leads typically to a choice that is also sufficiently above the threshold rate for stability. The theory described is also useful to derive more general digital filters and to perform the design of stable LDI filters exclusively in the Z-plane without reference to analogue prototypes.
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    Circuits, systems and signal processing 11 (1992), S. 285-307 
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    Notes: Abstract In this paper we describe a family of operators for constructing nontrivial left and right coprime factorizations under rather weak conditions for a large class of nonlinear feedback control systems that has a stabilizable or unstabilizable plant, using a common criterion of input-output stability. The proposed sufficiency and construction method are based on a generalization of the nonlinear Lipschitz operator theory formulated by us for such systems. This class of generalized nonlinear Lipschitz operators constitutes a very large family (an infinite-dimensional Banach space) of bounded nonlinear operators that describe (part of) the underlying systems. One of the main difficulties in constructing coprine factorizations for nonlinear feedback systems has been in taking care of the nonlinear composite and inverse operators that appear in the closed-loop configuration such that the overall feedback system is well defined in the sense of stability, causality, and uniqueness of the internal signals and such that the coprime factorizations can be achieved. In this paper we show how these difficult issues can be handled nicely under our framework of generalized nonlinear Lipschitz operator theory, at least for a very large class of nonlinear control systems. We give a simple illustrative example to show how these coprime factorizations can actually be characterized and constructed to yield explicit closed-form solutions.
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    Circuits, systems and signal processing 11 (1992), S. 387-398 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract In this paper we study the problem of designing a neural network that gives the correct binary representation of a given real number. Previously this problem has been studied by Tank and Hopfield. The network proposed by them exhibits “hysteresis” in the sense that the current vector of the network sometimes converges towards a binary vector that isnot the correct binary representation of the input current. The reason for this is that the network proposed by them has multiple asymptotically stable equilibria. In the present paper, we propose another neural network which has the property that it hasa single, globally attractive equilibrium for almost all values of the input current. Hence, irrespective of the initial conditions of the network, the current vector converges towards the correct binary representation of the input current.
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    Circuits, systems and signal processing 11 (1992), S. 421-430 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Concepts of observability and strong observability for singular systems are introduced and characterized geometrically in terms of the system matrices without using the Weierstrass decomposition. Duality relations between observability and controllability and that between reachability and strong observability are established. A canonical form for the action of the output injection group on the set of observable systems is presented.
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    Circuits, systems and signal processing 11 (1992), S. 455-492 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Mathematical morphology is becoming increasingly important in industrial vision applications for object recognition and defect inspection. In this paper we define a new function based on mathematical morphology, which we named the Rotationally Invariant Pecstrum (RIP). The RIP is proven to be invariant under rotation regardless of the shape of the structuring element. This paper also discusses how to use the RIP as an object descriptor and studies the effect of using different structuring elements on recognizing objects of different shapes.
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    Circuits, systems and signal processing 11 (1992), S. 493-508 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract This work presents close-form formulas for typical two-dimensional bandselect linear-phase FIR filters (e.g., band-pass, high-pass, and band-stop filters) optimal in the least-squares sense. Cases of both circular and elliptical symmetry are considered. The formulas refer to the coefficients of the frequency sampling form of the frequency response. Therefore they can either be directly used for filter implementation, if the frequency sampling structure is adopted, or used to obtain the impulse response coefficients via an inverse FFT.
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    The Geneva risk and insurance review 17 (1992), S. 7-191 
    ISSN: 1554-9658
    Keywords: Risk ; Insurance Demand ; Deterministic Transformations ; Stochastic Dominance ; Expected Utility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract The consequences of a change in a random parameter are determined for a decision model with more than one source of randomness. The two cases of independent and stochastically dependent sources of risk are discussed. Four comparative static theorems are given. These state the effect of first degree stochastically dominant shifts or risk decreases for one random variable while the other random variable is held fixed. Deterministic transformations are used to represent random parameter changes. The results are presented in the context of the coinsurance demand model with a risky insurable asset and background risk.
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    The Geneva risk and insurance review 17 (1992), S. 21-33 
    ISSN: 1554-9658
    Keywords: Insurance Demand ; Multiple Sources of Risk ; Comparative Statics ; Increases in Risk
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    Topics: Economics
    Notes: Abstract In this paper we propose an answer to the following problem of comparative statics in models with multiple sources of risk: How a risk averse agent will change his coinsurance demand when the distribution of the insurable loss is shifted? To answer the question, we first comment on Jack Meyer's results and then we show how an alternate approach leads to more definitive comparative statics.
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    The Geneva risk and insurance review 17 (1992), S. 35-60 
    ISSN: 1554-9658
    Keywords: Ambiguity ; Pricing ; Actuaries ; Decision Processes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This paper reports on the results of a questionnaire study of pricing decisions made by professional actuaries when the probabilities of a loss are either ambiguous or nonambiguous. Theoretical hypotheses derived from the expected utility model were compared with the implications of procedures described by practicing actuaries. Actuaries were asked to act as consultants to a computer manufacturer concerning the price of a warranty. The suggested prices were considerably higher when probabilities were ambiguous than when they were well-specified. These pricing decisions were consistent with the procedures described by actuaries but inconsistent with predictions from expected utility theory when the risks are perfectly correlated. Further insight into the actual decision process is provided by interviews with actuaries and an analysis of comments written on the questionnaire forms.
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    The Geneva risk and insurance review 17 (1992), S. 61-75 
    ISSN: 1554-9658
    Keywords: Saving ; Borrowing ; Increases in Risk Aversion ; Increases in Risk ; Multivariate NonExpected Utility Model
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    Topics: Economics
    Notes: Abstract We adopt the multivariate non-expected utility approach proposed by Yaari [1986] to provide a characterization of the comparative statics effects of greater risk aversion and of mean-preserving increases in risk on saving and borrowing in the presence of income and interest rate risk. We show that in Yaari's model, it is possible to extend the applicability of the Diamond and Stiglitz [1974] and Kihlstrom and Mirman [1974] (DSKM) single-crossing property to establish a relationship between greater risk aversion and saving (or borrowing) on the basis of the individual's ordinal preferences as long as the two risks are independent. We also demonstrate that the comparative statics effects of a joint mean-preserving increase in random income and interest rate on saving and borrowing can be determined by an extension of the DSKM single-crossing property.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 37-43 
    ISSN: 1476-5535
    Keywords: Linear alkylbenzene sulfonate ; Biodegradation ; Plasmid ; Detergent ; Gene probe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Linear alkylbenzene sulfonate (LAS) is a widely used anionic surfactant. Although approximately 1 million metric tons of LAS are produced annually, relatively little is known about the bacteria or the genetic factors that control LAS degradation in the environment. The objectives of this research were to: i) compare bacterial populations in wastewater and pristine pond systems; ii) determine the frequency of plasmids in bacteria from these sites; and iii) compare the frequency of DNA sequences coding for aromatic catabolism in isolates from these two sites. Plate counts indicated that exposure to wastewater resulted in higher levels of both heterotrophic bacteria and bacteria capable of growing on LAS containing medium (LAS/YEPG). In addition to higher numbers, a higher proportion of heterotrophs from the wastewater system were capable of growth on LAS/YEPG medium. Thus, the high levels of LAS in the wastewater system apparently selected fro organisms that were able to tolerate and/or degrade, it. Mineralization of14C-ring labelled LAS in any habitat related to the presence of organisms that grew on LAS/YEPG. Although may of these isolates could carry out primary degradation, no isolate, could mineralize14C-ring LAS in pure culture. A higher incidence of plasmids was found in bacteria from the wastewater pond and among bacteria that grew on LAS containing medium. However, the presence of plasmid, DNA did not necessarily confer the ability to degrade LAS nor was the ability to degrade LAS dependent on the presence of a plasmid. The incidence of selected genotypes for aromatic catabolism was similar among isolates on LAS/YEPG at both sites, suggesting that LAS ring degradation may be present in other populations or encoded by alternative sequences. In conclusion, LAS mineralization is mediated by a consortium and the evidence that initial attack of LAS is plasmid mediated is inconclusive.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 103-107 
    ISSN: 1476-5535
    Keywords: Fatty acid bioconversion ; hydroxy octadecenoic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Previously, we reported the discovery of a new compound, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) which was produced from oleic acid by a new bacterial isolate PR3 [6,7]. The reaction is unique in that it involves a hydroxylation at two positions and a rearrangement of the double bond of the substrate molecule. Now, we have isolated another compound from the reaction mixture determined by GC/MS to be 10-hydroxy-8-octadecenoic acid (HOD). NMR and IR data indicate that the unsaturation is probablycis. The optimum pH and temperature for the production of HOD by strain PR3 were 6.5 and 30°C, about the same as those for DOD. However, the amount of HOD detected remained small throughout an 48-h reaction period during which the amount of DOD increased sharply. At 48 h of reaction, the ratio between HOD∶DOD was 1∶10. HOD may be an intermediate in the biosynthesis of DOD from oleic acid.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 109-113 
    ISSN: 1476-5535
    Keywords: Candida blankii ; Biomass ; d-Xylose ; l-Arabinose ; Acetate
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary All fourCandida blankii isolates evaluated for growth in simulated bagasse hemicellulose hydrolysate utilized the sugars and acetic acid completely. The utilization ofd-xylose,l-arabinose and acetic acid were delayed by the presence ofd-glucose, but after glucose depletion the other carbon sources were utilized simultaneously. The maximum specific growth rate of 0.36 h−1 and cell yield of 0.47 g cells/g carbon source assimilate compared with published results obtained withC. utilis. C. blankii appeared superior toC. utilis for biomass production from hemicellulose hydrolysate in that it utilizedl-arabinose and was capable of growth at higher temperatures.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 115-119 
    ISSN: 1476-5535
    Keywords: Mycolytic enzymes ; Trichoderma viride ; Protoplasts ; Cochliobolus lunatus ; Fermentation
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Microorganism useful for the induction of enzymes lytic towards walls of filamentous fungusCochliobolus lunatus were studies. Production of specificTrichoderma viride mycolytic enzymes was studied in a laboratory fermentor. The product with high chitinase and relatively low protease activity gave better yields ofC. lunatus protoplasts than commercial Novozym 234.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 121-125 
    ISSN: 1476-5535
    Keywords: Trichoderma viride ; Cellulase production ; Optimized production medium and parameters
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A 25-l scale protocol is devised for the optimal secretion and recovery of fungal cellulase. Using a selected higher yieldingTrichoderma viride SMC strain, a protocol consisted of: a) an optimized production medium rich in microcrystalline cellulose (MCC), fortified with 1% (w/v) ammonium sulphate, 0.5% (w/v) soybean flour, 0.1% (v/v) Tween-80 and other trace nutrients; b) optimized physical parameters of production, such as an inoculum containing a homogeneous suspension of 6×107 conidia per 1,28±1°C, pH 4.0±0.5, 300±20 rpm, 11000±1000 l/h aeration, and 170–220 h duration; c) optimal recovery through a filter press (450 l/h rate of filtration) followed by precipitation with 2.5–3.0 volumes of acetone (15°C and basket centrifugation (27°C, 1700 rpm)); and d) vacuum drying (35°C, 4–6 h). This afforded 70% recovery of cellulase in the form of white fluffy powder containing 20000±2000 carboxy methyl cellulase and 1000±50 units filter paperase per g activities, with raw material cost of US$ 8–10 per million carboxy methyl cellulase units. During storage for 18 months at 4°C, ambient temperature and 37°C, the cellulase preparation was found to retain 100, 75 and 60% of its initial activity, respectively.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 131-135 
    ISSN: 1476-5535
    Keywords: Cheese ; Starters ; Production ; Alignate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Calcium alginate beads containingLactococcus lactis cells were used for three batch fermentations of milk or a commercially available growth medium (Gold Complete, Nordica) with the aim of producing concentrated cultures. Repeated fermentations did not significantly increase bead CFU counts which were between 3.3–7.8×1010 CFU/g. During the second and third fermentations, which lasted 6 h each, the bead populations decreased if the incubation was extended over 2 h. There was cell release from the beads. Fermentation media and fermentation time all had an effect on free cell counts, but none of these factors statistically interacted. Free cell counts were higher at the end of fermentations 2 and 3 than in the first fermentation and approximately 50% of the population was in the free state. Free cell counts were higher when the beads were incubated in Gold complete than in milk. Although the total bacterial population of a standard free cell fermentation was always higher than those having immobilized cells, immobilized cell technology did enable the production of dense cultures.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 247-250 
    ISSN: 1476-5535
    Keywords: Fructosyl-transferring enzyme ; 1-Kestose ; Fructo-oligosaccharide ; Continuous production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary β-Fructofuranosidase P-1 fromAureobasidium sp. ATCC 20524, which produces a fructo-oligosaccharide (1-kestose) from sucrose, was immobilized covalently onto alkylamine porous silica with glutaraldehyde at high efficiency (44.4%). Optimum pore diameter of porous silica for immobilization of the enzyme was 91.7 nm. The enzymatic profiles of immobilized enzyme were almost identical to the native one except its stabilities to temperature and metal ions were improved. 1-Kestose was produced continuously and selectively from 40% (w/v) sucrose at fast flow rates by a column packed with the immobilized enzyme for up to 26 days, and the effluent concentration of 1-kestose remained in the range 113–135 mg ml−1.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 257-260 
    ISSN: 1476-5535
    Keywords: Polysaccharide ; Fructan ; Gum ; Fermentation ; Bacillus polymyxa ; Sweetener
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Bacillus polymyxa (NRRL-18475) produced a levan-type fructan (B, 2→6 fructofuranoside) when grown on sucrose, sugarcane juice, and sugarbeet molasses. The organism converted about 46% of the fructose moiety of sucrose to levan when grown on sucrose medium, however, the yields of levan from sugarcane juice and beet molasses were much less than sucrose solution. Such sugarcane juice and beet molasses can be made a good substrate for levan production by various modifications. Adding peptone to sugarcane juice or passing beet molasses through a column of gel filtration media improved levan yield to a level almost comparable to that obtained from sucrose.
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    Circuits, systems and signal processing 3 (1984), S. 267-294 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Pipeline techniques have been successfully applied to speeding up processing in both general- and special-purpose digital computers. Application of these techniques to nonrecursive (FIR) filters has been suggested and is quite straightforward. Application to recursive (IIR) filters has not previously been shown. In this paper, the technique for applying pipeline techniques to recursive filters is shown and the advantages and disadvantages of the technique are discussed. Using these techniques, recursive digital filters operating at hitherto impossibly high rates can be designed.
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    Circuits, systems and signal processing 3 (1984), S. 295-314 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The long-standing problem of reconstructing a function from its samples is considered again. Assuming a sequence of oversampled values, a set of appropriate idealized reconstruction filters can be defined, which do not suffer from instability or slow convergence. The realization — a cascade of a nonrecursive digital filter, D/A-converter, and a fixed/analog smoothing filter — demands the design of the digital filter for the increase of the sampling rate. The design of this nonrecursive filter is the purpose of this paper. Approximations in the frequency as well as in the time domain are presented.
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    Circuits, systems and signal processing 11 (1992), S. 3-4 
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    Circuits, systems and signal processing 11 (1992), S. 5-6 
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    Topics: Electrical Engineering, Measurement and Control Technology
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    Circuits, systems and signal processing 11 (1992), S. 431-439 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract An efficient design of digital differentiators (DD), with a variable frequency range of operation at low and midband frequencies, has been proposed. Implemented as a Taylor structure, a DD designed for orderN can be made to function as a universal configuration giving optimal transfer functions for all possible ordersN, without changing the coefficients.
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    Circuits, systems and signal processing 3 (1984), S. 105-122 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract In this paper we formulate power systems as nonlinear nearly Hamiltonian systems. Using the invariance principle for ordinary differential equations, necessary and sufficient conditions for asymptotic stability are established and a new method of estimating the domain of attraction of the stable equilibrium point is developed. The present results constitute a novel approach to stability analysis and involve the following three steps: a. Given a system with dissipation, the stability of its equilibrium is ascertained by determining the stability of the associated conservative system. b. Attractivity of the stable equilibrium of the entire system (with dissipation) is determined from the system topology. c. An estimate of the domain of attraction of the asymptotically stable equilibrium is obtained by making use of results obtained in (a) and (b). The stability criterion developed in this paper sheds new light on the mechanism of instability in power systems and it provides analytical verification to the concept of the potential-energy boundary surface (PEBS). The PEBS is a hypersurface which makes up a part of the boundary of the domain of attraction of the stable equilibrium in a power system. The existence and properties of the PEBS have thus far been deduced primarily via simulations and heuristic methods.
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    Circuits, systems and signal processing 3 (1984), S. 161-176 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract In conventional television systems, picture scanning in vertical and temporal directions is usually very defective with regard to the sampling theorem. In this paper some deficiencies such as aliasing, line-structure distortion, line flickering, and large-area flickering are investigated with regard to their dependence on the inter-lacedpicture-scanning process. The three-dimensional reconstruction filtering of the sampled picture is especially analyzed with respect of the viewer's perception. Furthermore, it will be shown that in connection with a new concept of picture scanning published earlier [1], [2], a flat-field reproduction without any 25/30-Hz flicker can be achieved by vertical filtering only. This is true even though the final reproduction by the monitor is performed with interlace. The vertical filtering can then be optimized in the sense of maximum picture sharpness and resolution with negligible ringing as well. Practical results are given in this paper.
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    Circuits, systems and signal processing 3 (1984), S. 177-191 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract In this paper a new type of “velocity-selecting/rejecting filter” which passes or stops a particular event in a seismic signal is proposed. The velocity-selecting filter is based on a time-space band-pass filter with sharp passband for a particular direction, and similarly, the velocity-rejecting filter is based on a time-space band-stop filter. A technique for designing such filters, in terms of an infinite-impulse-response (IIR) filter, is presented, in which a rotated version of separable filter is used. Finally, numerical examples are included to illustrate the design theory.
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    Circuits, systems and signal processing 3 (1984), S. 347-359 
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    Topics: Electrical Engineering, Measurement and Control Technology
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    Circuits, systems and signal processing 3 (1984), S. 315-325 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract A special type of factorization for operators defined on partially ordered Hilbert resolution spaces is considered. The main result includes, as a particular case, the classical Schur-Coleski triangular factorization. Connections with stochastic optimization and image-processing problems are established.
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    Circuits, systems and signal processing 3 (1984), S. 409-417 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract In a recent paper on nonlocal expansions necessary and sufficient conditions are given under whichf −1 has a generalized power series expansion, whenf is an invertible locally Lipschitz map between certain general subsets of a complex Banach space. Here we establish the validity of a conceptually interesting algorithm for obtaining the expansion. Basically, we show that a certain contraction mapping iteration generates iteratesℐ 1,ℐ 2,... such that eachℐ k yields all of the terms of the generalized power series expansion off −1 up to order (k + 1), assuming merely that the expansion off −1 exists. An earlier different result along related lines is mentioned.
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    Circuits, systems and signal processing 3 (1984), S. 447-475 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The representation of functions in a basis function expansionz(t)= ∑k=1/∞=,a k〉 x k (t) is straightforward when the basis functionsx k (t) are orthogonal. There has been very little work up to this time in determining how to use nonorthogonal bases in signal representation. On the other hand, applications in data compression and signal synthesis often require using specific tailor-made bases. Presented here is a method for constructing very general nonorthogonal bases. Orthogonality has often been used to show that a basis spans the set of functions of interest and to calculate the coefficients of the representation. In this paper, both of these fundamental aspects are addressed for nonorthogonal bases. A new basis {y k (t)} is obtained by performing a linear transformation on a known existing basis {x k (t)}. This transformation is constructed such that the coefficients of signal representation on the new basis are readily found. Then, a useful and sufficient condition is placed upon the new basis such that representations converge. The fundamental methods are applied to the standard examples of signal representation. The complex sinusoids, the Rademacher functions, the orthogonal polynomials, and the decaying exponentials are used as the original basis {x k (t)} from which a new basis {y k (t)} is generated. Two examples are given to illustrate general applications: one in signal synthesis and one in signal analysis.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 11-17 
    ISSN: 1476-5535
    Keywords: Eicosapentaenoic acid ; Mortierella elongata ; Omega-3 fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary WhenMortierella elongata NRRL 5513 was cultured in shake flasks at 25°C, mycelial growth reached a stationary phase at 48 h but maximum eicosapentaenoic acid (EPA) production was observed at 6 days. When incubated at 11°C, EPA production also continued to rise during the stationary phase of growth, reaching a maximum after 10 days. An initial culture pH of 6.1 was found to be optimum for EPA production. The effect of temperature on EPA production was dependent on medium constituents. In glucose and linseed oil supplemented media, optimum temperature for EPA production was 11 and 15°C respectively. A maximum EPA yield of 0.61 g/l was obtained in linseed oil (2%), yeast extract (0.5%) supplemented basal medium. Maximum EPA content as a percentage of lipids (15.12%) was observed when the latter medium was supplemented with 0.25% urea.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 1-9 
    ISSN: 1476-5535
    Keywords: Recombinant culture ; Dissolved oxygen ; Biomass ; Plasmid content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Four recombinant strains ofEscherichia coli were examined for the effects of the dissolved oxygen level on the level of biomass, the plasmid content, and the level of recombinant protein at the stationary phase of batch growth. Strains JM101/pYEJ001, and TB-1/pYEJ001 (encoding chloramphenicol acetyltransferase), and strain TB-1/p1034, and TB-1/pUC19 (encoding β-galactosidase) were grown at the constant dissolved oxygen levels of 0, 50, and 100% air saturation, as well as in the absence of dissolved, oxygen control. The biomass of all strains under constant aerobic conditions was 12–36 times higher than that under anaerobic conditions, but was the same as or slightly higher than that without dissolved oxygen control. The plasmid content in all strains under anaerobic conditions was 2.9–11.7 times higher than that under aerobic conditions. The optimal dissolved oxygen concentration for the specific activity of recombinant proteins was dependent upon the strain. In no strain were constant aerobic conditions optimal. However, because of the effect on biomass, controlled aerobic conditions were optimal for the volumetric activity of recombinant protein in all but one strain.
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    Journal of industrial microbiology and biotechnology 9 (1992), S. 19-25 
    ISSN: 1476-5535
    Keywords: Schizophyllum commune ; Sclerotium glucanicum ; Branched β-1,3-glucan ; Fan-impeller ; Oxygen limitation ; Process design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Glucan formation ofSchizophyllum commune andSclerotium glucanicum were investigated. Process data obtained during batch cultivation are presented. Glucan release can be improved by oxygen limitation. Thus, growth and glucan release are influenced by oxygen in opposite ways. Possible pathways of this oxygen-dependent regulation are discussed. A draft-tube/propeller system, rushtonturbine-, fan- and helicon-ribbon-impeller as well as a fundaspi and intermig agitator were tested. The 4-bladed fan impeller withd *=0.64 yielded the best results, since effective bulk mixing is much more important than bubble break up (micromixing) with regard to this system. Fed-batch cultivation always resulted in higher rates of glucan formation than the batch process.
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  • 97
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 9 (1992), S. 27-36 
    ISSN: 1476-5535
    Keywords: Genetically engineered microorganisms ; Bovine somatotropin ; Survival ; River
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The fate in water ofEscherichia coli K-12 strain LBB269, both plasmid-free and carrying the recombinant plasmid pBGH1, was studied.E. coli K-12 strain LBB269 (pBGH1) is a nalidixic acid resistant derivative of W3110G (pBGH1), the microorganism used by Monsanto Company for the commercial production of bovine somatotropin. Water samples were obtained from the Missouri River and from the Monsanto Life Sciences Research Center aqueous waste basin. Strains LBB269 and LBB269 (pBGH1) were grown in fermentation vessel under bovine somatotropin (BST) production conditions, and inoculated into the water samples. The inoculated water samples were incubated, at 26°C, and the number of viableE. coli cells was determined as a function of time. In sterile water from both sources, the two strains remained, at a constant level for at least 28 days; LBB269 (pBGH1) remained at a constant level in sterile water for at least 300 days. In non-sterile water from both sources, the two strains declined from an initial concentration of about 3.0×106 cells per ml to less than 10 cells per ml in 147 h. The study conditions did not adversely affect the populations of indigenous microorganisms. The selective loss of strains LBB269 and LBB269 (pBGH1) demonstrates that theseE. coli strains do not survive in environmental sources of water. In addition, it was observed that the presence of pBGH1 had essentially no effect on the disappearance of strain LBB269 from either source of water.
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  • 98
    ISSN: 1476-5535
    Keywords: Protein kinase C (PKC) ; Phorbol ester ; Methylpendolmycin ; Pendolmycin ; Actinomycete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary During the course of screening for inhibitors of phorbol ester binding to protein kinase C, several actinomycete cultures were discovered that produce active metabolites. HPLC coupled to photodiode array and LC/MS techniques were applied to broth extracts to identify the presence of indolactams belonging to the teleocidin family. Various members of this family were rapidly identified from crude broth extracts using a combination of these spectroanalytical procedures. An analytical HPLC system was developed to optimize separation of teleocidin, A and B analogues directly from ethyl acetate extracts of whole broth cultures. This technique allowed us to identify a novel homologue of pendolmycin and demonstrated the utility of photodiode array HPLC coupled with LC/MS as an intial analytical tool in the analyses of these secondary metabolites produced by soil microorganisms.
    Type of Medium: Electronic Resource
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  • 99
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 9 (1992), S. 53-61 
    ISSN: 1476-5535
    Keywords: Bioremediation ; Biotransformation ; Cytochrome P-450 ; Metabolism ; PAHs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The polycyclic aromatic hydrocarbons (PAHs) are a group of hazardous environmental pollutants, many of which are acutely toxic, mutagenic, or carcinogenic. A diverse group of fungi, includingAspergillus ochraceus, Cunninghamella elegans, Phanerochaete chrysosporium, Saccharomyces cerevisiae, andSyncephalastrum racemosum, have the ability to oxidize PAHs. The PAHs anthracene, benz[a]anthracene, benzo[a]pyrene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene, as well as several methyl-, nitro-, and fluoro-substituted PAHs, are metabolized by one or more of these fungi. Unsubstituted PAHs are oxidized initially to arene oxides,trans-dihydrodiols, phenols, quinones, and tetralones. Phenols andtrans-dihydrodiols may be further metabolized, and thus detoxified, by conjugation with sulfate, glucuronic acid, glucose, or xylose. Although dihydrodiol epoxides and other mutagenic and carcinogenic compounds have been detected as minor fungal metabolites of a few PAHs, most transformations performed by fungi reduce the mutagenicity and thus detoxify the PAHs.
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  • 100
    ISSN: 1476-5535
    Keywords: α-Amylase ; Histidine ; Chemical modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The α-amylase ofBacillus caldovelox is inactivated by diethyl pyrocarbonate at pH 6.6 and 20°C by a monomolecular reaction with a second-order rate constant of 41.7 M−1·min−1. The rate of inactivation increases with decreasing pH, suggesting participation of an amino acid residue with a pK a of 6.6. The increase in absorbance at 240 nm, unchanged absorbance at 280 nm and reactivation in the presence of hydroxylamine suggest the participation of a histidine residue. Statistical analyses of inactivation suggest that only one histidine residue is essential for activity. Substrate afforded complete protection against inactivation, indicating the involvement of the histidine residue at the active site of the enzyme.
    Type of Medium: Electronic Resource
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