ALBERT

Notes: A novel hybrid stubble turnip, Appin, bred by the Scottish Plant Breeding Station (Brassica campestris ssp. rapifera cv. Tigra ×B. campestris ssp. nipposinica cv. Mizuna) was compared in small-plot cutting trials with stubble turnip (B. campestris ssp. rapifera cv. Ponda), forage rape (B. napus ssp. biennis cv. Canard) and fodder radish (Raphinus sativus cv. Neris) for 3 years, 1975–77, samples being taken from October to December or January each year to cover the likely period of utilization by grazing lambs. Except for the first year, Appin proved to yield less DM than Ponda, and be inferior in digestibility and metabolizable energy (ME) content, though N contents were on occasion higher. Canard had the highest overall ME content. In dry conditions in autumn 1977, Ponda proved the more reliable crop, suffering less depression in yield than other species. Anti-metabolite contents were determined in freeze-dried samples taken in winter 1976-77 and showed Canard to have the most brassica anaemia factor (S-methyl cysteine sulphoxide): contents of thiocyanate were generally similar between crops.As the degree of utilization of such forages when grazed can outweigh differences in recorded above-ground DM yields from cutting trials, it was considered that claims of better root anchorage and hence better utilization for Appin warranted evaluation in a grazing trial.

Notes: Two grass silages made from perennial ryegrass, and with D-values of 0·216 and 0·255, were offered ad libitum to 18 Ayrshire cows in two feeding experiments. On the control treatment the silages were supplemented with soybean meal only, and on the other two treatments with equal weights of DM from either barley or dried molassed sugar-beet pulp plus the same weight of soya as on the control treatment. The daily intakes of silage DM were not significantly different on the barley and beet-pulp treatments, and, on average, the intake of silage DM was reduced by 0·24 and 0·20 kg by feeding 1 kg barley and beet pulp DM respectively.The daily milk yields were not significantly different on the barley and beet-pulp treatments with mean values of 19·2 and 19·2 kg per cow respectively compared with 17·2 kg on the control treatment. On the barley and beet-pulp treatments the fat, SNF, CP and lactose concentrations in the milk and the live weights of the cows were not significantly different. It is concluded that the barley and beet pulp had similar feeding values and replacement rates when used as supplements with grass silage, and that the two feeds were interchangeable on an equal DM basis.

Notes: An experiment examining the relationship of daily herbage disappearance (DHD) and daily herbage allowance (DHA) is described. Cows with calves were grazed at three stocking rates on Kleingrass and Coastal Bermudagrass pastures. DHD and DHA were monitored at 14-d intervals. A significant positive linear relationship was noted between DHD and DHA (P〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:01425242:GFS9:les" location="les.gif"/〉0·25). Equations derived for Coastal Bermudagrass and Kleingrass were not significantly different and resulted in a combined equation Ŷ= 0·275X + 0·209 with an r2 vaiue of 0·27. As DHA exceeded 6–9 kg DM per 100 kg live weight, efficiency of defoliation by the cows and calves declined.

Notes: The effects of five herbicides on seed yield and viability of perennial ryegrass cv. S24 were tested at two sites. Benzoylprop-ethyl, chlorfenpropmethyl, difenzoquat, ethofumesate and flam-prop-isopropyl were safe to use at doses recommended for use in other crops. When applied at three times the recommended dose, only difenzoquat reduced the yield and germination of seed and benzoylprop-ethyl reduced seed germination. The recommended doses of difenzoquat and ethofumesate were safe when applied on cv. Barlenna at different growth stages. Difenzoquat reduced seed yield only after three times the recommended dose was applied to tillered plants. Ethofumesate, even at three times the recommended dose, did not affect seed yield but plant numbers were reduced by early treatment. The results suggest that the herbicides tested may be safe to use in perennial ryegrass seed crops at the recommended doses but more work is needed on the safety of difenzoquat in relation to crop growth stage.

Notes: The digestibility of herbage selected by dairy cows, milk-fed calves, weaned calves and wether sheep was examined on four occasions each day as they strip-grazed together a daily allocation of herbage. The average quality of the material selected by each group was similar. Organic matter digestibility of the herbage selected declined from 0·274 to 0·223 as the average height of the sward fell from 16 to 6 cm; this decline was slightly more rapid for the cows than for the other stock. A similar trend was also evident in samples cut from the sward to simulate the horizon removed prior to each sampling time.

Notes: Grass silage made in May from S24 perennial ryegrass had a DM concentration of 262 g kg-1 and an in vitro D-value of 0·698, and was given ad libitum to twelve Ayrshire cows in a 16-week feeding experiment. In addition supplements of hay with a mean in vitro D-value of 0·603 were offered in one of three forms—long, chopped (12·1 mm) or ground (0·80 modulus of fineness) and cubed—and of concentrates given at either 2 or 4 kg per 10 kg milk. Hay averaged 18·2% of total forage DM intake with mean daily intakes of 1·28, 1·22 and 2·26 kg DM per cow in the long, short and ground forms respectively. The highest daily intakes of forage, i.e. silage plus hay, occurred on the ground hay treatments, with values of 10·24 and 9·25 kg DM per cow on the 2 and 4 kg concentrate treatments respectively. The mean daily milk yields were 18·2,18·2 and 19·2 kg per cow on the long, short and ground hay treatments respectively but the increase in yield with the ground hay was only significant at the low level of concentrate intake. The hay treatments had small and non-significant effects on milk composition. It is concluded that ground hay was superior to either long or chopped hay as a supplement for silage, but the small advantages depended on the level of concentrate intake.

Notes: Book review in this article Energy from Biomass in Europe Edited by W. Palz and P.

Notes: The water loss from tall fescue cv. S170, perennial ryegrass cv. S24, Italian ryegrass cv. RvP and timothy cv. S352 when dried as a thin layer under controlled conditions at 20°C and r.h. 50% is described. Comparisons were made on six occasions between mid-April and mid-June. Tall fescue dried faster than the other species and the time to reach a water concentration of 0·2 g water per g dry matter varied little between the six harvests. In contrast, the drying time for the ryegrasses and timothy increased to maximal values in mid-May and then fell. Drying time was dependent on the initial water concentration of the grass, the ratio of leaf to stem and on the amount of true stem exposed to the drying environment. The practical implications in relation to haymaking are discussed.

Notes: The results are reported of an examination of detailed measurements on the ingestive behaviour of calves and lambs in response to variations in the surface characteristics of swards under strip-grazing and continuous stocking managements. It is shown that intake per bite and the short-term rate of herbage intake were both sensitive to the height of the surface horizon above ground level but, at least under strip-grazing management, not to variations in herbage density in the grazed horizon. The rate of biting was less sensitive to variations in sward conditions, particularly under strip-grazing.Both intake per bite and rate of intake were more sensitive to variations in grazing height under strip-grazing, where sward changes were rapid, than under continuous stocking, where they were slow. Under continuous stocking, ingestive behaviour was more sensitive to changes in sward conditions in lambs than in calves.

Notes: In an experiment of four years duration, the competitive relationships between three cultivars of white clover (Trifolium repens L.) and ten cultivars of perennial ryegrass (Lolium perenne L.) were studied under a N fertilizer regime of 200–240 kg ha-1 a-1. The clover cultivars were selected to embrace the various leaf sizes from medium large to small and the ryegrasses included early, mid-season and late cultivars with a known range of persistence.During the experiment there was a progressive decline in the contribution of clover, though the different characteristics of each of the clover and ryegrass cultivars produced substantial deviations from the average trend. The clover cultivar Kersey was significantly more aggressive towards the companion grass cultivars than either S100 or S184. It produced consistently greater clover contribution to total yield than the other two cultivars and significantly depressed the yields of some of the companion grass cultivars. There was evidence that compatibility of the ryegrass cultivars with clover was inversely related to persistence; the non-persistent ryegrass cultivars S321 and Presto consistently produced lower grass yields than the more persistent cultivars and consequently permitted greater clover contribution. In the second and third years yield substitution effects between clover and grass components substantially reduced differences in the total grass-clover yield.The interactions revealed in the experiment showed that both ryegrass and clover cultivars have the potential to influence each other when in association although, with minor exceptions, total annual yields were similar for all grass and clover mixtures at the moderately high level of N applied.

Notes: White clover (cv. S184 or Blanca) or marsh trefoil (cv. Grasslands 4703 or Grasslands Maku) were sown alone and in combination with a companion grass (tall fescue, red fescue, perennial ryegrass or ryegrass cleanings) on a peat area of hill land originally dominated by Juncus articulatus and Molinia caerulea.Lime and ground rock phosphate were applied in 1973, the area rotavated to a shallow depth and seeds sown in May 1974. No fertilizers were applied except at sowing time and two harvests were taken per annum for 4 years.Differences in yield and legume content between companion grass treatments were small. After the first full harvest year Blanca swards usually had the lowest DM yields and those containing Maku the highest. The contribution of marsh trefoil to total herbage yield increased from 4·2% in 1975 to 22% in 1978, whereas white clover decreased from 4·2 to 1·2%. Legume yields in 1978 were less than half those in 1977. Overall, N yields were low, Maku swards fixing most N estimated to be highest in 1977 at 35 kg ha-1.It is concluded that marsh trefoil grows well on upland peat but more information on its response to grazing is necessary before conclusions can be drawn about its value in hill land improvement.

Notes: An experiment extending over a 2½-year period from June 1976 to October 1978 assessed the effect on herbage yield of method of establishment, species and level of N application.Perennial ryegrass cv. Gremie and Italian ryegrass cv. RvP were established by broadcasting or drilling into cultivated ground or by direct-drilling, using a Howard Rotaseeder, into paraquat-sprayed but uncultivated ground. These treatments were combined with annual rates of N application of 0, 120, 240 or 360 kg ha-1.Over the experiment drilling into cultivated ground gave the highest yield—3·6 and 4·8% higher than the broadcast and direct-drilled treatments, respectively. Italian ryegrass consistently outyielded perennial ryegrass, with an overall mean dry matter yield advantage of 23·7%. There was no significant interaction between sowing method and species; direct-drilled Italian ryegrass produced a mean yield 17·3% higher than that of drilled perennial ryegrass.

Notes: The length of life of the root axes of a plant was taken as the interval between the prevention of the plant from producing any further axes and the death of the shoot. Of the cultivated species studied Lolium perenne had the shortest lived roots and Dactylis glomerata the longest with Phleum pratense, Festuca arundinacea, F. pratensis and F. rubra intermediate. Holcus lanatus and Nardus stricta had long lived roots comparable with Dactylis glomerata. The ranked order of species is in general agreement with the observations reported in the literature.

Notes: Flowering behaviour of plants of six cultivars of Trifolium repens (Kent Wild White, Grasslands Huia, S100, Blanca, Olwen and Milkanova) growing in field plots at the Grassland Research Institute, Hurley, was recorded in mid-July. Half of each plot had been defoliated in early May, the other half in mid-June. In all cultivars defoliated in May, inflorescence initiation had stopped even though the natural photoperiod at that time was well above the critical daylength for initiation Defoliation in mid-June had led to a renewal of inflorescence initiation in all cultivars.The results confirm that the stimulatory effect of defoliation on inflorescence initiation in plants that have stopped initiation in long days, which has previously been observed in Grasslands Huia in controlled environments, also occurs in the field and in other cultivars.

Notes: The mineral composition of Lolium perenne, Dactylis glomerata, Trifolium repens, Lotus corniculatus and six non-leguminous broadleaved herbs was determined at two sites on copper-deficient soils with and without soil-applied copper sulphate at 2 kg Cu ha-1. D. glomerata was richer than Lolium perenne in P, Mn and Cu, and T. repens was richer than either grass species in Ca, P, Mg and Cu. D. glomerata cultivars differed in Ca, P, Na and Mg concentrations, and L. perenne cultivars in Ca concentration. Lotus corniculatus contained less Ca, P, Na and Mn but more Zn than T. repens. Achillea millefolium was rich in K, Cichorium intybus in K, Mg and Zn, Petroselinum crispum in Na and Zn, Rumex acetosa in K, P, Mg and Zn and Taraxacum officinale in Na, Mg and Zn. Rumex was low in Na and Plantago lanceolata in Na and Mn. None of these herbs was richer than T. repens in Cu. Copper sulphate application raised the Cu concentration of the herbage only slightly, species or varieties differing little in their response to applied Cu. It is concluded that adjustment of botanical composition, with or without copper sulphate application, will not help to prevent hypocupraemia in grazing stock.

Notes: Two experiments are described in which groups of lactating cows, non-lactating cattle and weaned calves grazed a sequence of swards varying in maturity and herbage mass under strip grazing management at a daily herbage allowance of 60 g dry matter per kg live weight. Lactating cows ate 43% and 76% more herbage than non-lactating cattle of similar weight in the two experiments but herbage OM intakes per unit live weight by the calves and lactating cows were similar.Variations in diet digestibility and herbage intake in the lactating and non-lactating cattle with changes in sward conditions were similar. In the first experiment the calves were experienced grazers; the variation in the digestibility of the herbage selected was less in the calves than the adult cattle, but the variation in herbage intake was greater. The calves in the second experiment were younger, and they were inexperienced grazers; their ability to increase herbage intake in response to changing sward conditions was poorer than that of adult cattle, though variations in diet digestibility were similar in all classes.The practical implications of these results are discussed briefly.

Notes: A mathematical model which simulates the response of grass to irrigation over a range of rainfall and soil conditions is described. Using the model the probable costs and benefits from investing in irrigation facilities for intensive beef and dairy production have been determined. Irrigation only appears to show a reasonable return on investment where the mean summer rainfall is less than 350 mm or the water-retaining capacity of the soil is low. Furthermore, investment in irrigation is only likely to be logical where the rate of fertilizer N applied is greater than 300 kg N ha-1. It also appears that a strategy of partial irrigation is unlikely to yield the maximum financial gain. Overall the indications are that for the foreseeable future only a small percentage of the total grassland area in the UK can be irrigated profitably.

Notes: Six red clover cultivars, three diploid—Essex, Sabtoron and Violetta—and three tetraploid— Teroba, Red Head and Hungaropoly—were sown alone and with each of three companion grasses—timothy (S48), tall fescue (S170) and perennial ryegrass (S24). The productivity and persistency of the red clover cultivars were compared over 4 years. Dry matter (DM) yield, DM digestibility and the crude protein (CP) concentration were assessed and botanical analyses conducted on herbage samples from each treatment at each of three harvests per annum. Annual fertilizer application consisted of 165 kg P and 312 kg K ha-1.Comparing clover cultivars alone Essex was significantly less productive and less persistent than the other five cultivars. Yield and persistency of the five other cultivars did not differ markedly within years with the exception that the diploids were significantly less productive than the tetraploids in the fourth year. Over all 4 years mean annual total DM and clover DM yields of the five cultivars were between 12·2 and 13·2 t ha-1 and between 9·2 (79·2% of total DM yield) and 10·2 (83·2%) t ha-1 respectively, and differences were not significant.Up to the end of the third year there was little or no advantage gained by the inclusion of a companion grass, annual total DM yields being between 11·2 and 14·2 t ha-1 for clover alone and between 10·2 and 14·2 t ha-1 for clover-grass mixtures. In the fourth year there was an overall tendency for the yield of the clover alone to be lower, between 7·2 and 12·2 t ha-1, than that of the clover-grass mixture, between 8·2 and 13·2 t ha-1, and this was more pronounced with the diploid than with the tetraploid clover cultivars. Sown with companion grasses, Essex and Hungaropoly were lower in yield and in contribution than the other cultivars over the 4 years. The influence of the companion grass on total dry matter yield showed that the contribution of timothy was low relative to that made by tall fescue and perennial ryegrass. Perennial ryegrass made the most varied contribution from year to year. Tall fescue was the most consistent contributor with all clover cultivars and at the end of 4 years both yield and clover-grass balance had not changed materially.No pronounced differences in DM digestibility were evident between treatments.Crude protein concentration of the pure clover was similar to that of the clover-timothy treatments and both would appear to be superior to either the clover-perennial ryegrass or clover-tall fescue mixtures.It is considered that red clover dominant swards are suitable for use under a cutting regime and can provide high yields of DM at a low cost for up to 4 years. Such swards are self-sufficient in N and in addition soil N accumulation can be exploited in the production of succeeding crops.

Notes: The effects of severity of grazing on the herbage intake and milk production of continuously stocked British Friesian cows calving in February–March were examined in three experiments conducted in the years 1976–78 (experiments 1–3 respectively) using a put-and-take technique. In experiment 1 four grazing severities were imposed by maintaining swards with different herbage masses (2500, 3000, 3500, 4000 kg OM ha-1); in experiments 2 and 3 there were two severities of grazing maintained by keeping swards canopies at constant heights of 5 and 7 cm (experiment 2) and 5 and 7·2 cm (experiment 3). Cows were reallocated to treatment every 8 weeks in experiments 1 and 2 and there were three periods, whereas they all grazed throughout a 23-week period on the same treatment in the final trial.A decrease in the quantity of herbage on offer or in sward height reduced herbage intake and milk production in all experiments. Mean daily herbage OM intakes were 11·2, 12·2, 12·2 and 12·2 kg respectively in experiment 1, 12·2 and 13·2 kg respectively in experiment 2 and 12·2 and 152 kg respectively in experiment 3. Mean daily solids–corrected milk yields were 14·2, 15·2, 15·2 and 16·2 kg respectively in experiment 1, 14·2 and 16·2 kg respectively in experiment 2 and 12·2 and 17·2 kg respectively in experiment 3. It was apparent from the data obtained in the first two trials that grazing at a sward canopy height of 7 rather than 9 cm had little effect, but that at 5 cm there were significant depressions in both herbage intake and milk production. Milk yield was depressed to a greater extent when cows were kept on the same treatment for the whole season.

Notes: Twenty-four Hereford × Friesian cows and their South Devon cross calves were allocated to three herbage allowances allotted daily for three periods of 8 weeks in a Latin square design. The daily allowances were 17, 34 and 51 g dry matter per kg cow plus calf live weight. Milk production was depressed by 0·2 and 1·2 kg d-1 at the medium and low allowances. The corresponding falls in liveweight gain were for cows 0·26 and 0·25, and for calves 0·27 and 0·24 kg d-1. Residual sward height after grazing gave a better indication of the animals' reaction to sward conditions and the management imposed than actual herbage allowance. The quantity per unit area and the composition of material present were important factors influencing intake. Calves were unable to compete with their dams to maintain herbage intake at the lower allowances and therefore are likely to benefit from additional feeding or creep grazing when residual sward height falls below 6cm for periods in excess of 1–2 weeks.

Notes: The data from 12 individual cattle grazed on a uniform sward were used to compare field sampling of faeces with three time-schedules for rectal sampling of faeces. Estimates of digestibility from a faecal nitrogen equation were compared with estimates based on in vitro digestibility, or modified acid-detergent fibre determinations, on clipped herbage samples.Field sampling gave a lower coefficient of variation (8%) in chromic oxide content than did grab sampling (14%). Grab sampling introduced an upward bias in estimates of faecal output compared with field samples. The estimate of digestibility based on the faecal index was intermediate to those based on clip samples from the upper and lower horizons of the sward. There was no difference in the mean digestibilities estimated from in vitro or MAD fibre.

Notes: Groups of eight Hereford × Friesian cows and their South Devon cross calves were set stocked over a 24-week grazing season at 3·23 (low), 3·21 (medium) or 4·24 (high) cows ha-1 together with their calves. For the first 8 weeks only two-thirds of the total area was grazed. Increasing the stocking rate from low to medium reduced daily milk yield and cow and calf liveweight gains by 1·2, 0·24 and 0·29 kg d-1 respectively, and from medium to high by 1·2, 0·24 and 0·23 kg d-1. The main sward factor influencing faecal output and herbage intake was the quantity of organic matter on the pastures but the digestibility of the herbage selected also exerted a significant effect on the intake of cows. Major depressions in the herbage intake of cows occurred once the average sward height fell below 7 cm. Output of calf live weight was 628, 658 and 743 kg ha-1 for the 3 stocking rates from low to high, and for cows 246, 179 and 30 kg ha-1. It was concluded that decisions on pasture management should be taken in relation to the cow rather than the calf on set-stocked swards.

Notes: Swards of L. perenne and P. annua were treated at the 2–3 leaf stage with recommended rates of ethofumesate (5 and 10 kg a.i. ha-1). P. annua ceased normal growth, became swollen at the shoot base and eventually showed signs of senescence. L. perenne showed no visible defects, treated swards being more vigorous than untreated. Scanning electron micrographs showed that the developing leaf primordia of P. annua had been affected by the ethofumesate. In both grass species, ethofumesate induced abnormalities in cell division. These abnormalities caused irreversible damage to P. annua but only slight distortion of L. perenne which later recovered and resumed normal growth. More detailed biochemical studies are required to determine the specific effects of ethofumesate on cell growth.

Notes: A description is given of equipment designed to be fitted to either sheep or cattle, and to record automatically information on the jaw movements associated with both grazing and ruminating, movements of the head in plucking mouthfuls of herbage, and the time spent in grazing. The equipment has been successfully tested against alternative visual and mechanical methods of measuring grazing behaviour.The use of modified equipment in detailed studies has shown (a) that the maximum acceleration of the head in a longitudinal plane when plucking mouthfuls of herbage was twice as great in sheep as in cattle and in the sheep, but not in the cow, increased as sward height decreased; and (b) that the ratio of jaw movements to head movements was always greater than unity in both sheep and cattle, and was greater on tall than on short swards in two out of three comparisons. The rate of biting was significantly lower on tall than on short swards.

Notes: In experiments to determine the minimum fertilizer requirements for improved pasture on deep peat, yields were initially low and declined rapidly. Within 2 years persistence of sown species was poor when only 2·5 t lime ha-1 was applied. Analytical data suggested that K deficiency and low soil pH were contributory factors. When K was omitted from a composite nutrient solution, clover yield was reduced by 50% when 2·5 t lime ha-1 was applied but was not significantly reduced with 5·0 t lime. Clover alone produced little response to either P or K separately, but highly significant positive interactions were recorded. Clover, but not ryegrass, responded to K topdressing in field cut-herbage experiments. There was a 3-fold increase in ryegrass yield with combined P and K topdressing under grazing; 10 times more N and K were recycled in urine on this treatment than on the control.It was concluded that at least 5·0 t lime, 60 kg P and 80 kg K ha-1 are required for pasture establishment and that soil pH should be maintained above 5·0 to minimize K requirements. The significance of nutrient cycling and of lime × K and P × K interactions is discussed in relation to the persistence of sown species and the maintenance of improved swards on deep peat.

Notes: Two growth experiments were carried out in January-March 1978 in which simulated swards of perennial ryegrass were (1) heated by soil warming cables to give soil surface temperatures of approx. 7·5-9·C above ambient temperature, (2) shaded with netting to reduce light levels by approx. 50%, or (3) both heated and shaded.Heating alone increased leaf appearance, death, extension, lamina size, leaf area index (LAI), tillering (month 1)and whole plant weight (month 2) and reduced stubble water-soluble carbohydrates and specific leaf weight (SLW).Shading alone increased leaf extension, lamina size and LAI but to a lesser extent than did heating. Shading decreased SLW, leaf death rate, tillering (month 2), stubble carbohydrates and whole plant weight, but not herbage weight.The effects of heating plus shading were similar to those of heating alone, except that the increases in leaf size, extension and LAI were even greater, and shoot bases and roots had low or negative growth rates.In general the heating treatments caused a rapid turnover of leaf material, but net herbage growth was relatively insensitive. It is concluded that (1) temperature rather than light was limiting whole plant growth, especially from mid-February to mid-March and (2) mild, dull weather in winter is likely to induce tiller death associated with reduced investment in carbohydrate reserves.

Notes: The experiment was undertaken to examine the interactions between yield responses to secondary applications of fertilizer N and length of regrowth interval following three levels of primary N. A sward of S24 perennial ryegrass received all combinations of three fertilizer treatments, nil, 50, and 100 kg ha-1 N for primary growth harvested on 21 May and four treatments, nil, 33, 66 and 100 kg ha-1 N for the second growth harvested 2, 3, 4, 5, 6 or 7 weeks later.Cubic equations of growth were derived from the yields of dry matter and both the direct response to secondary N and the residual responses to primary N increased as regrowths developed. A positive residual effect to increasing primary N was obtained at the three lower levels of secondary N for weeks 4–7 but a negative response was obtained with the highest level of secondary N. The implications are discussed in terms of target responses and yields and the growth interval required to attain these targets.

Notes: Four grass silages of two chop lengths and two D-values were made from S23 perennial ryegrass, and compared in a feeding experiment with lactating cows and in an eating-behaviour study with non-lactating cows. The median chop lengths were 11·6 and 11·5 mm in the short silages and 18·2 and 19·5 mm in the medium silages. Chop length had negligible effects on D-value and on pH with values of 3·81 and 3·94 on the short and medium silages respectively.Silage DM intakes were higher on the short than on the medium-chop silages in both experiments except where the high D-value silage was supplemented with concentrates. Milk yields were not affected significantly by chop length with daily values of 19·3 and 19·7 kg per cow on the short and medium treatments respectively with the high D-value silage, and 18·2 and 18·3 kg per cow with the low D-value silage. Chop length had only small and non-significant effects on milk composition.Eating times expressed as min per kg DM were significantly lower on the short than on the medium-chop silages.It is concluded that the small differences between the short- and the medium-chop silages were of no economic importance.

Notes: The practise of sward desiccation using glyphosate or paraquat followed by direct drilling of seed gave an environment which increasingly inhibited germination and establishment when the interval between spraying and drilling was decreased from 21 days to nil. Burning or removal of old sward improved establishment. Calcium peroxide used as a seed dressing improved seedling establishment in most treatments.

Notes: Book review in this article Tropical Pastures and Fodder Crops By L. R. Humphreys The Role of Nitrogen in Intensive Grassland Production Edited by W. H. Prins and G. H. Arnold. Agricultural Botany, 1. Dicotyledonous Crops, 2. Monocotyledonous Crops, By N. T. Gill and K. C. Vear, Third edition revised by K. C. Vear and D. J. Barnard

Notes: A DNA hybridization assay was used to detect isolates of Escherichia coli that have the genetic potential to produce toxin. This method was compared with the traditional Y-1 mouse adrenal cell method for detecting pathogenic E. coli isolated from humans. Results showed that the DNA hybridization method has the potential to detect foodborne bacterial pathogens. The advantages and disadvantages of this genetic method are compared with those of the traditional assays for pathogenicity.

Notes: Salmonella typhimurium H391 neurotoxin was sensitive to gamma radiation under in vivo conditions but resistant under in vitro conditions. Cells were detoxified after exposure to a radiation dose of 500 krad while isolated neurotoxin retained toxicity up to a dose of 3,000 krad. Detoxification of S. typhimurium H391 cells after exposure to 500 krad was accompanied by loss of antigenic properties which may be attributed to changes in the physico-chemical behavior of the neurotoxin moiety after in vivo radiation.

Notes: The presence of monolauryl-glyceride (monolaurin) in the heating menstruum (phosphate buffer, reconstituted non-fat dry milk or cream style corn) of Bacillus stearothermophilus 1518 spores increased the rates of spore inactivation at 113–121°C by 2–3 fold. The ZD-values for the spores heated with and without monolaurin were 8.7 and 6.9°C, respectively. Rates of inactivation of B. subtilis A spores also were enhanced, but spores of Clostridium perfringens NCTC 879B, C. sporogenes PA 3679, C. botulinum 62A, or C. botulinum 213B were unaffected.Increasing the concentration of monolaurin from 0.4 mM to 3.6 mM increased the rate of inactivation, but concentrations higher than 3.6 mM did not appear to influence the effectiveness of monolaurin. All monoglyerides containing C8-C16 saturated fatty acids appeared to increase the inactivation of B. stearothermophilus spores, but the enhancement of spore inactivation declined as the fatty acid chain length increased beyond 10 carbon atoms. The influence of monolaurin on B. stearothermophilus spores did not appear to be pH dependent over the range of pH 6 to 8.The spore-monolaurin interaction was not due to heat-injury of the spores, although the interaction was dependent on the heat treatment. The increased inactivation appeared to be due to a heat enhanced chemical inactivation of the spores.

Notes: Antimicrobial activity of fatty acids and their glycerol and sugar esters against a film-forming yeast isolated from raw soy sauce was determined in diluted soy sauce. In a series of fatty acids (alkyl chain length of 7, 9, 11 and 13) and their monglycerides, capric acid and monolaurin had the highest inhibitory activity. Two selected sugar esters (sucrose monocaprate and sucrose monolaurate) could not completely inhibit the growth of test organism throughout 3 weeks.

Notes: A study was conducted to determine the effects of sorbic acid and several derivatives of fatty acids (amides, aminimides, and monoglycerides) upon toxigenic cultures of Aspergillus flavus and A. parasiticus. A synthetic medium was inoculated with spores, incubated for 48 h at 27°C, and then supplemented with sorbic acid and fatty acid derivatives. Cultures were then incubated for an additional 5 days. Aflatoxins were extracted, separated, and quantitated. Mycelial mats were dried, weighed, and analyzed for lipid and mineral content. Cerulenin (8 μg/ml) was the most effective fatty acid derivative examined, reducing mycelial growth by 37% and completely inhibiting extracellular accumulation of aflatoxins. Other derivatives, in decreasing order of effectiveness, included M-20 (an aminimide), lauribic, and lauricidin. Mycelia grown in the presence of fatty acid derivatives contained less phosphorus, potassium, magnesium, phosphatidyl ethanolamine, phosphatidyl serine, cholesterol, and triglycerides, but more cardiolipin, phosphatidyl choline, free fatty acids, fatty acid esters, and diglycerides. Levels of monoglycerides and cholesterol esters remained essentially unchanged. Inhibition by sorbic acid was nonspecific, affecting both mycelial growth and extracellular aflatoxin accumulation to approximately the same extent. Utilization of fatty acid derivatives for determining mechanisms of aflatoxin accumulation and lipid biosynthesis appears promising.

Notes: The nutritional requirements for the synthesis of a cholera toxin-like toxin from Salmonella enteritidis serotype braenderup were examined. Toxin levels of both culture filtrates and sonicates were determined by the modified Chinese hamster ovary (CHO) floating cell assay. With few exceptions, most amino acids which contained charged polar R groups were stimulatory for Salmonella toxin synthesis. Glycerol enhanced toxin production to the largest extent; however, glucose was a poor carbon source suggesting that Salmonella toxin elaboration may be subject to catabolite repression. Biotin and Mn++ were shown to increase the synthesis as well as release of this toxin. An ideal defined medium, supplemented with all the nutritional factors shown to stimulate toxin synthesis, was formulated. The amount of Salmonella toxin produced in this supplemented medium was about 82% of the total toxin elaborated when cultured in Casamino acids plus yeast extract.

Notes: Five plating media, Hektoen enteric (HE) and xylose lysine deoxycholate (XLD) agars with and without 80 and 5 μg/ml of novobiocin (N), respectively, and brilliant greeen sulfadiazine (BGS) agar with 80 μg/ml of the antimicrobial agent, were analyzed for the recovery of salmonellae from various fresh beef, pork, and poultry meat products. Of the total Samonella positive samples, 50.0% and 82.5% were found on XLD and XLD-N agars, respectively, 75.0% and 85.0% on HE and HE-N agars, respectively and 65.0% on BGS agar. HE-N and BGS media isolated three times more false positives than did XLD-N agar, while XLD and HE agars gave the highest numbers of false positives. The major H2S producing false positive on XLD and HE agars was Proteus mirabilis. With the addition of N, P. mirabilis was eliminated, and the major H2S producing false positive was almost exclusively Citrobacter freundii. The false positives on BGS agar were predominately distributed among C. freundii, Enterobacter sp., and Klebsiella sp.

Notes: Cynomologus monkeys (Macaca fascicularis) were tested for their sensitivity and specificity to staphylococcal enterotoxin A (SEA). Thirty-two of 38 monkeys vomited within 5 h in response to intragastric feeding of 4.8–18 μg of crude SEA. Twenty-four of these 32 responding monkeys were subjected to specificity study by feeding crude SEA which was neutralized with specific Antiserum A. Twenty-two (92%) of the 24 demonstrated specificity by not vomiting when fed neutralized crude SEA. The remaining two (8%) monkeys showed specificity only with purified SEA neutralized with the Antiserum. The emetic dose -50 for crude SEA was 6.5 μg per monkey. These suggest that cynomologus monkeys are suitable for SE bioassay and for identification of new enterotoxins.

Notes: A kinetic study was performed on the combined effect of monolaurin and heat on the death of Escherichia coli. The following results were obtained: (1) Monolaurin was about eleven times more active than that of sodium laurate; (2) Temperature enhanced the effect of monolaurin. True enthalpyentropy compensation effect was shown in this death reaction. The value of 341.9°K was obtained from the formula of ΔH*= Tc·ΔS*+ b and 331.5°K from Arrhenius plots as a compensation temperature; (3) The apparent minimum enhancing concentration of monolaurin ranged from 0.0056 mM to 0.013 mM, varying with the heating temperature. It may be concluded from the results of this study that the enhancing effect of monolaurin on the thermal death of E. coli corresponds to that of the amphoteric surfactant type (S type) agent defined in our previous report.

Notes: The antimicrobial effects of monolaurin and sorbic acid were compared in laboratory media, cottage cheese and a pork homogenate. Sorbic acid (250–1000 ppm) was more effective than monolaurin (250–1000 ppm) in inhibiting the growth of Staphylococcus aureus S-6 and Salmonella enteritidis 13076 in pH 5.2 trypticase soy broth. Monolaurin was more effective than sorbic acid against the growth of S. typhimurium 13311 under similar conditions. No synergistic effects were observed when combinations of monolaurin and sorbic acid were tested. In the cottage cheese study, 800 ppm sorbic acid effectively inhibited the growth of coliforms and yeasts and molds, but only slightly inhibited the growth of psychrotrophs. Monolaurin at 800 ppm was ineffective in controlling the growth of any of the microorganisms tested. The use of 3000 ppm monolaurin in a pork homogenate had no effect on the outgrowth and gas formation by Clostridium sporogenes PA3679, whereas 3000 ppm sorbic acid delayed outgrowth and gas formation. Combinations of monolalurin and sorbic acid indicated that sorbic acid was the active anticlostridial agent.

Notes: Twenty kinds of ciguatoxic fishes such as grouper, moray eel, barracuda, catfish and snapper, etc. were collected from fish markets in Bangkok, Chanthaburi and Chonburi. Liver and meat were extracted with ether and the presence of ciguatoxin was checked by the mouse test. Ciguatoxin was not detected in any of the samples. On the other hand, water-extracted fractions under acetic acidic conditions from meat, skin, intestine, liver and ovary of five kinds of puffer collected from Chanthaburi, Samut Sakorn and Trat caused mouse paralysis or death. The ovaries of the green rough-backed blowfish and the starry blowfish, which are very popular in Thailand, contained large amounts of tetrodotoxin. The starry blowfish contained 1482.0 mouse units of tetrodotoxin per gram of ovary.

Notes: A review of plasmids in Clostridium perfringens is presented. The characterization of the caseinase mediating plasmid pHB101 based upon limited DNase treatment, restriction endonuclease treatment, and agarose gel electrophoresis is described. Antibiotic profiles for the wild type strain and the cured strain were determined. The plasmid pHB101 was non self-transferable. The curing procedure resulted in a stable morphological change from rod to coccoid or bacillary-coccoid shape. A screening of C. perfringens strains showed the general presence of a 9.4 Mdal plasmid which may have been overlooked in the past due to chromosomal DNA masking effects.

Notes: Book reviewed in this article Food Safety. Edited by H. R. Roberts.

Notes: Repeated (five) doses of pyridoxine chloride (vitamin B6, 10 mg) reduced botht he neurological symptoms caused by ethylidene gyromitrin, the main poisonous compound of the mushroom false morel (Gyromitra esculenta pers. Fr.), and the overall mortality due to ethylidene gyromitrin poisoning in mice treated orally with this poison (400 mg/kg). In contrast, little or no effect was found when the mice were treated with γ-aminobutyric acid (1 mg) or vitamin C (10 mg) after treatment with poison. The significance of these findings is discussed in perspective to possible treatments of human poisonings caused by false morel.

Notes: Glyceryl monolaurate when used in the proportion of 5 g per kg of meat slurry (pH 6.0–6.2) inhibited toxin production by Clostridium botulinum type A (strain 73A), type B (strain OKRA) and type E (strain RIV 2). The concentration of glycerol monolaurate required for inhibition was not reduced by addition of lactic acid to the meat slurry until the pH was lowered to pH 5.2 or below. In meat slurry (pH 6.0–6.2), potassium sorbate inhibited production of type B toxin at 32 g/kg. The addition of butylated hydroxy-anisole to glyceryl monolaurate had no effect upon the concentration needed for inhibition of botulinum toxin production.

Notes: A lipid-enveloped virus (Semliki Forest virus: SFV) and a nonenveloped virus (coxsackievirus B4) were treated with neutral lipids known to be present in human milk or able to be generated by lipase activity in human milk. Triglycerides and diglycerides were inactive compared to monoglycerides and free fatty acids which were active against SFV (90% inactivation) at concentrations as low as 0.7 μM. Of the compounds tested monolinolein was the most active against SFV. None of the lipids tested were active against coxsackievirus B4. A synthetic milk (Almiron-M2), high in unsaturated triglycerides, was inactive against either virus.

Notes: A cultivar of Lolium perenne L. (Causeway) selected for tolerance to foliar-applied paraquat was shown also to be tolerant at seed germination and establishment.When seeds were germinated in paraquat solutions (1, 3, 9, 27 ppm), Causeway was more than ten times as tolerant as a normal cultivar of L. perenne.When germination and survival were studied in a soil which had been sprayed with paraquat (0.0, 0.3, 0.9, 2.7 kg/ha) before sowing, Causeway was nearly three times as tolerant as a normal cultivar. Holcus lanatus L. was about equal to normal L. perenne, but Poa trivialis L. was less tolerant. Phytotoxicity was greater in a 10.5% organic than a 4.8% organic soil.It is concluded that the mechanism of paraquat tolerance operates in the non-photosynthetic tissues of germinating seedlings as well as in the photosynthetic tissues of older plants, although the degree of tolerance varies according to the mode and stage of application of the herbicide. There might be advantages in using a paraquat tolerant cultivar of L. perenne when direct drilling grass after sward destruction by paraquat.

Notes: Spring-produced seeds of Lamium amplexicaule L. were buried in pots of soil in an unheated glasshouse in June 1978, and at 1–2-month intervals, for 27 months, they were exhumed and tested for germination in light and darkness at temperatures simulating those in the habitat from early spring to late autumn. Freshly-matured seeds were dormant, but by autumn 85% or more germinated in light at 15/6, 20/10, 25/15 and 30/15°C but only 7% or less in darkness. During late autumn and winter germination in light decreased at 25/15 and 30/15 °C but not at 15/6 and 20/10 °C, and germination in darkness increased at 15/6 and 20/10 °C. During late winter and early spring germination in light at 15/6 and 20/10 °C decreased, and seeds lost the ability to germinate in darkness. By the second autumn of burial, seeds germinated to near 100% in light at 15/6 to 30/15 °C and to 10–25% in darkness at 15/6 and 20/10 °C. The cycle of germination responses was repeated during the second winter and spring and the third summer of burial. Autumn-produced seeds were dormant when buried in November 1979, but by spring they germinated to 81 and 36% at 15/6 and 20/10 °C, respectively, in light. These seeds afterripened further during summer. The consequence of seasonal changes in germination responses is that (1) seeds can germinate in the habitat in late summer, autumn and spring but not in early- to mid-summer or in late autumn and winter and (2) during both germination seasons, seeds produced during the previous spring(s) and/or autumn(s) can germinate.

Notes: Linuron (0.21 and 0.28 kg/ha) and linuron + MCPA (0.21+0.56 kg/ha) in a tank mixture with field rates of barban, difenzoquat and flamprop-methyl reduced the phytotoxicity of these herbicides to Avena fatua. When linuron was applied immediately following or 6 days after the A. fatua herbicides no reduction in phytotoxicity to A. fatua occurred, suggesting that the antagonism may be occurring as a result of physical or chemical incompatability when the herbicides are mixed together. The possibility of obtaining broad-spectrum weed control with one trip over the field by applying linuron and one of these wild oat herbicides separately but at the same time using a double-boom, double-tank system deserves evaluation. When linuron was applied in a tank mixture (0.21 and 0.28 kg/ha), immediately after, or 6 days after diclofop-methyl (0.70 kg/ha), there was no reduction of A. fatua control, and wheat tolerance to the tank mixture was good. This tank mixture offers potential for control under field conditions of A. fatua and some broad-leaved weeds in one spray operation. Linuron + MCPA (0.21+0.56 kg/ha) in a tank mixture severely reduced A. fatua control with diclofop-methyl. No loss of phytotoxicity to Fagopyrum tataricum occurred when the A. fatua herbicides tested were tank mixed with linuron or linuron + MCPA. Lutte contre Avena fatua et Fagopyrum tataricum avec des mélanges extemporanés de linuron ou de linuron + MCPA et des applications successives de linuron et d'herbicides de postlevée actifs contre A. fatua

Notes: The effect on seed dormancy of exposing the mother plants to different environmental conditions was studied in Datura ferox. Reducing incident radiation and withholding water to provoke water stress, during the period from fruit set until seed ripening, caused a decrease in the degree of dormancy of the harvested seeds. A significantly smaller amount of the basic fraction inhibitors was found in the seeds with the lesser degree of dormancy. It is suggested that one of the ways in which the environmental conditions to which the mother plants are exposed can influence seed behaviour is by changing the endogenous inhibitor levels

Notes: A bioassay technique based on Poa annua growth showed that ethofumesate applied at 2.0 kg/ha in October to a newly-sown ryegrass (Lolium perenne) sward had a half-life of just over 8 weeks. After 7 months there was still sufficient herbicide present to cause about a 50% reduction in P. annua growth. The movement of ethofumesate, as measured in soil cores taken 15 months after spraying, was confined mainly to the upper 2 cm of the soil profile. Seeds on the soil surface at spraying were very sensitive to ethofumesate. The implications of the findings for weed control in newly-sown grass leys are discussed.