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  • Autoradiography  (21)
  • Springer  (21)
  • 1990-1994
  • 1980-1984  (21)
  • 1925-1929
  • 1981  (21)
  • 1926
Collection
Keywords
Publisher
Years
  • 1990-1994
  • 1980-1984  (21)
  • 1925-1929
Year
  • 1
    Electronic Resource
    Electronic Resource
    Incorporation of35S-sulphate in chick blastoderms during elongation and during shortening of the primitive streak (1981)
    Springer
    Development genes and evolution 190 (1981), S. 233-236 
    Details
    ISSN: 1432-041X
    Keywords: Sulphate ; Glycosaminoglycan ; Autoradiography ; Gastrulation ; Chick
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chick blastoderms were cultured for 2 h in the presence of35S-sulphate. The distribution of the grains after light microscope autoradiography was compared in blastoderms during the elongation and during the shortening of the primitive streak. A uniform labeling was observed over the cells in both groups. Accumulation of grains was present in both groups at the ventral side of the upper layer, where transmission electron microscope studies have revealed a basal lamina. An additional accumulation of grains occurred over the cells and in the extracellular spaces of the head process and of the rostral part of blastoderms with shortening primitive streaks. This positivity could be correlated with the presence of ingressing and recently ingressed notochordal cells. Treatment of the sections with chondroitinase ABC and/or HNO2 before dipping in the nuclear emulsion demonstrated that at least chondroitin sulphate and N-sulphated heparan sulphate were present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848309
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  • 2
    Electronic Resource
    Electronic Resource
    Synthesis of the intranuclear crystals in Scolopendrium vulgare (L.) SM (1981)
    Springer
    Planta 152 (1981), S. 137-144 
    Details
    ISSN: 1432-2048
    Keywords: Autoradiography ; Intranuclear crystals ; Nucleus, crystolo-Protein transfer-Pteridophytes ; Scolopendrium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Proteinaceous intranuclear crystals are found in the fern Scolopendrium vulgare. During mitosis these crystals are eliminated from the nucleus into the cytoplasm, where they are dissolved. New crystals appear in the nucleus. The site of synthesis of intranuclear crystal proteins was investigated using quantitative ultrastructural autoradiography after incubation with tritiated lysine. The results suggest a migration of cytoplasmic proteins to the nucleus, part of which would then be incorporated into the intranuclear crystals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391185
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  • 3
    Electronic Resource
    Electronic Resource
    Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat (1981)
    Springer
    Cell & tissue research 216 (1981), S. 215-220 
    Details
    ISSN: 1432-0878
    Keywords: Autoradiography ; 3H-proline ; Osteoblasts ; Sympathectomy ; Guanethidine-sulfate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sympathectomy was carried out in rats by injections of guanethidine-sulfate from birth to 14 days of age. At 45 days of age, the activity of osteoblastic cells was monitored by 3H-proline autoradiography. Effectiveness of sympathectomy was verified by light-microscopic examination of superior cervical and celiac ganglia. Grain counts over periosteal osteoblasts of the femoral diaphysis and osteoblasts mesial to the first molar in the mandible demonstrated a significantly reduced uptake of 3H-proline in the sympathectomized rats. The data provide direct evidence of sympathetic influence on osteoblastic activity and suggest that sympathectomy may result in the loss of a trophic influence which is important in the regulation of osteogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234556
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural morphometry of blastogenesis (1981)
    Springer
    Cell & tissue research 215 (1981), S. 643-649 
    Details
    ISSN: 1432-0878
    Keywords: T-lymphocytes ; Blast cells ; Autoradiography ; Electron microscopy ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled with tritiated thymidine. Random samples of cells were taken for light and electron microscopic morphometry. In comparison to the unstimulated lymphocyte, the stimulated cell increased in size and possessed a greater plasma membrane surface area. Increase in cell size was the result of increases in the volumes of all measured subcellular compartments both in the nucleus and the cytoplasm. Heterogeneity of the stimulated cell population precludes firm conclusions regarding the significance of all these ultrastructural changes, though alterations in cell surface are discussed in the context of known biochemical differences accompanying blastogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233538
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  • 5
    Electronic Resource
    Electronic Resource
    Hyphal growth ofHypomyces chlorinus Tul.: An autoradiographic study (1981)
    Springer
    Protoplasma 107 (1981), S. 221-233 
    Details
    ISSN: 1615-6102
    Keywords: Autoradiography ; Cell wall synthesis ; Chitin ; Hypomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The incorporation of the chitin precursor N-acetyl-D-(1-3H) glucosamine byH. chlorinus has been studied by light and electron microscopic autoradiography. Light microscopic autoradiography showed that the incorporation occured preferentially at the hyphal apex. Autoradiograms from electron microscopy were quantitatively evaluated to determine the relative radioactivity incorporation between the cell wall and cytoplasm: this showed that (3H) incorporation took place mainly in the plasmalemma-wall complex. However a small amount of N-acetyl glucosamine can enter into the cytoplasmic space and is then transported by endomembranes (Golgi apparatus-vesicles) to the plasmalemma-cell wall interface before polymerization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276827
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  • 6
    Electronic Resource
    Electronic Resource
    Histochemical, cytochemical and autoradiographic studies on the rostellum ofHymenolepis diminuta (1981)
    Springer
    Parasitology research 64 (1981), S. 335-345 
    Details
    ISSN: 1432-1955
    Keywords: Hymenolepis diminuta ; Autoradiography ; Ultracytochemistry ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Histochemical studies on the rostellum ofHymenolepis diminuta revealed diastase-stable, protein/neutral carbohydrate-rich material localized in the rostellum tegument. The remainder of the rostellum, primarily composed of the glycogen-rich myocytons of the rostellum musculature, is protein-poor, but rich in diastase-labile, neutral and acidic carbohydrates. Ultrastructural cytochemical studies, using the periodic acid-thiocarbohydrazideosmium (PATCO) technique, indicated that the granules of the rostellar tegumental cytons and distal cytoplasm are carbohydrate-rich. Lipids are present in the rostellar myocytons but not in the tegumental cytons. Autoradiography using a pulse-labeling with [3H]leucine revealed an apical translocation of tegumental granules, but at a slower rate than had been reported for the strobilar tegument of the same organism. Neither [3H]galactose nor [3H]glucose were incorporated into the rostellar tegumental granules. The function of the secretory glycoprotein(s) produced in the rostellar tegument and its (their) possible role in the regulation of maturation and/or strobilization remain enigmatic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927380
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  • 7
    Electronic Resource
    Electronic Resource
    Observations on cytoplasmic transport along ovarian nutritive tubes of polyphagous coleopterans (1981)
    Springer
    Cell & tissue research 220 (1981), S. 153-161 
    Details
    ISSN: 1432-0878
    Keywords: Cytoplasmic transport ; Insect ovary ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarioles of Coccinella and Tenebrio are shown to be telotrophic — a characteristic normally associated with hemipterans rather than coleopterans. They possess an anterior region of trophic cells and a chain of oocytes. The trophic cells are connected with the latter by a series of nutritive tubes, and autoradiography has shown that RNA is transported along the tubes to the oocytes. However, the system in these beetles differs markedly from that of hemipterans in that the nutritive tubes do not contain an extensive complement of aligned microtubules. The significance of this to both the mechanism and the selectivity of transport is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209974
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  • 8
    Electronic Resource
    Electronic Resource
    Bilaterally asymmetrical uptake of [14C]2-deoxyglucose by the octavo-lateralis complexes in flatfish (1981)
    Springer
    Cell & tissue research 214 (1981), S. 659-662 
    Details
    ISSN: 1432-0878
    Keywords: Vestibular system ; Postural control ; Deoxyglucose method ; Autoradiography ; Flatfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary [14C]2-deoxyglucose uptake by neurons located in the octavo-lateralis complex of adult flatfish is asymmetrical on the two sides of the brain. It appears that the neuronal activity on the side oriented upward is higher than that on the side of the brain facing downward. This finding may be significant with respect to the mechanisms of metamorphosis of flatfish and may account for the peculiar fact that these animals swim on one body side during adult live.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233505
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured human small-intestinal tissue (1981)
    Springer
    Cell & tissue research 215 (1981), S. 1-12 
    Details
    ISSN: 1432-0878
    Keywords: Colchicine ; Human enterocyte ; Glycoprotein transport ; Autoradiography ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured biopsy specimens of the human intestine was investigated by light- and electron-microscopical autoradiography and by biochemical methods. The results showed a decrease in the radioactivity of the cell coat on the microvilli and an increase in the Golgi apparatus and in the apical vesicles and tubules. This divergence is attributed to a colchicine-induced impairment of the normal transport of cell-coat glycoproteins from the Golgi apparatus, via the apical vesicles and tubules, to the apex of the cell. The radioactivity of the lysosome-like bodies in the absorptive cells cultured with colchicine also increased. This finding supports a crinophagic function of these organelles in the degradation of excess cell-coat material. The investigations were supported in part by the Foundation for Medical Research (FUNGO), which is subsidized by the Netherlands Organisation for the Advancement of Pure Research
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236244
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  • 10
    Electronic Resource
    Electronic Resource
    Induction by progesterone of a sexual dimorphism of estrogen uptake by anterior pituitary cells in situ (1981)
    Springer
    Cell & tissue research 215 (1981), S. 75-86 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary cells ; Estrogen ; Progesterone ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of progesterone pretreatment on in vivo 3H-estrogen uptake by five anterior pituitary cell types was analyzed by means of a quantitative autoradiographic-immunocytochemical technique. Male and female rats castrated for 14 days show nuclear concentration of label in all five cell types one h after injection of 3H-estradiol, whether progesterone treated or not. The order of labeling intensity is gonadotropes ≧ lactotropes = somatotropes 〉 thyrotropes = corticotropes. Progesterone treatment induces a dramatic sexual dimorphism in estrogen uptake; it significantly increases 3H-estrogen uptake in all female cell types. In males, progesterone decreases uptake in gonadotropes while not altering uptake in other cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236250
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  • 11
    Electronic Resource
    Electronic Resource
    Monoamine precursor uptake in ganglia of a mollusc Anodonta cygnea L. (Bivalvia) (1981)
    Springer
    Cell & tissue research 219 (1981), S. 403-415 
    Details
    ISSN: 1432-0878
    Keywords: Monoamine precursors ; Ganglia ; Molluscs ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In vitro-uptake and localization of 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine have been investigated by means of light microscopic autoradiography in the central nervous system of the freshwater mussel, Anodonta cygnea L. Accumulation of the labelled monoamine precursors could be observed both over neuronal perikarya and neuropil in the ganglia. 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine were taken up by neurons characterized by a small size (10–20 μm), a polygonal form and an intense staining with toluidine blue. Labelled axon branches situated either in the neuropil or between nerve cells in the cortex exhibited only a moderate activity, frequently of a diffuse character. After pretreatment for 24 h with colchicine, silver grains were also seen over the neuronal perikarya and some axon profiles. This shows that local uptake of the monoamine precursors and, presumably, the synthesis of the monoamines takes place both at perikaryal and axonal/terminal level. The nerve cells accumulating the monoamine precursors contain a nucleus with rich chromatin content and a deeply invaginated nuclear envelope. Further ultrastructural characteristics of these neurons are their well-developed rough endoplasmic reticulum system, a great number of mitochondria and glycogen granules, and the presence of dense-cored vesicles of different types. All these features are responsible for the electron-dense appearance of the cytoplasm.
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    URL: http://dx.doi.org/10.1007/BF00210158
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  • 12
    Electronic Resource
    Electronic Resource
    In vitro binding of 125I-HCG to the rat ovary from birth to puberty (1981)
    Springer
    Cell & tissue research 214 (1981), S. 633-640 
    Details
    ISSN: 1432-0878
    Keywords: Rat ovary ; 125I-HCG binding ; Autoradiography ; Hormone receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neither homogenates nor frozen ovarian sections of rats aged 1, 5 and 7 days demonstrated specific binding of 125I-HCG. However, from day 10 to day 35, the homogenates could bind specifically, with an equilibrium association constant in the range of 0.9–2.7 × 1010 M−1. The binding capacity increased from 5.8 fmol/mg tissue at day 10 to 15.7 fmol/mg tissue at day 35. As revealed by autoradiography, the frozen ovarian sections from 10-day-old rats showed 125I-HCG binding localized over the interstitial and thecal tissues and, in the older age groups, also in the granulosa cells of follicles larger than 500 μm in diameter. These results indicate that LH/HCG receptors appear in the rat ovary at the beginning of the second postnatal week, and that the interstitial cells are the main site of action of LH before puberty.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233502
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  • 13
    Electronic Resource
    Electronic Resource
    Localization of 3H-dihydrotestosterone in the pituitary gland of the rhesus monkey (1981)
    Springer
    Cell & tissue research 215 (1981), S. 499-504 
    Details
    ISSN: 1432-0878
    Keywords: Androgen receptors ; Pituitary gland ; Rhesus monkey ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake and retention of radiolabelled dihydrotestosterone by the pituitary gland was examined in the rhesus monkey. Two animals were given an intravenous injection of 1.0μg/kg 3H-dihydrotestosterone (DHT) alone while one monkey received both the labelled androgen and 100μg/kg of unlabelled steroid. One and a half hours later, they were sacrificed. The pituitary glands were removed and processed for autoradiography and immunocytochemistry. Autoradiographic localization of DHT was discernible in the partes nervosa, intermedia and distalis, albeit the highest concentration of radiolabelled cells was noted in the pars distalis. Immunocytochemical staining with antibodies to rat PRL, human TSHβ and ovine LHβ revealed a population of steroid-concentrating cells that contained TSH and a second group that contained LH. None of the cells that reacted with the anti-PRL serum were radiolabelled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233526
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  • 14
    Electronic Resource
    Electronic Resource
    Evaluation of the effects of sensory denervation on osteoblasts by 3H-proline autoradiography (1981)
    Springer
    Cell & tissue research 217 (1981), S. 569-576 
    Details
    ISSN: 1432-0878
    Keywords: Autoradiography ; 3H-proline ; Osteoblasts ; Denervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The inferior alveolar nerve was unilaterally resected in 30-day-old mice; other animals were unilaterally sham-operated. At 15, 30, 60, 90, or 150 days after surgery, the mice were injected with 2μCi of 3H-proline (sp. act. 1.0 Ci/mM) per g of body weight and killed 15, 30, or 60 min later. Autoradiographs were prepared from 5μm decalcified sagittal sections of mandibles and grain counts made over periosteal osteoblasts mesial to the first molar. In denervated mandibles, osteoblasts incorporated less isotope compared to controls with differences being maximal at the early intervals. These differences became attenuated with time, possibly due to an intrinsic compensatory mechanism, secondary to neurotrophic regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219365
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  • 15
    Electronic Resource
    Electronic Resource
    Germinal centers and the B-cell system (1981)
    Springer
    Cell & tissue research 218 (1981), S. 59-73 
    Details
    ISSN: 1432-0878
    Keywords: Germinal centers ; B-cell differentiation ; Lymphocyte migration ; Autoradiography ; Lymphoid tissue ; Appendix (rabbit)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The migration pattern of germinal center cells of the rabbit appendix was studied and compared with that of appendix dome cells, spleen cells, thymus cells and thoracic duct lymphocytes. To discriminate T-and B-cell migration pathways, normal or T-cell-depleted rabbits were used as donors. Cell suspensions were labeled in vitro with 3H-leucine followed by intravenous transfer. The migration of labeled cells in lymphoid organs was studied using autoradiography, particular attention being paid to the spleen of the recipient. B-cells from the appendix dome, spleen and thoracic-duct lymph migrate to primary follicles or the corona of secondary follicles via thymus-dependent areas of peripheral lymphoid organs. In contrast, a B-cell subpopulation from the germinal centers of the appendix migrates to the center of splenic primary follicles and into germinal centers. The migration of germinal center cells to splenic follicle centers is not enhanced by specific antigens. The migration properties of B-cells, possibly changing during differentiation, may be instrumental in the two types of immune reactions, i.e., plasma-cell reaction and germinal-center reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210091
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  • 16
    Electronic Resource
    Electronic Resource
    Localization of 3H-estradiol in the uterus of pregnant and non-pregnant armadillos (1981)
    Springer
    Cell & tissue research 220 (1981), S. 773-780 
    Details
    ISSN: 1432-0878
    Keywords: Estrogen receptors ; Uterus ; Armadillo ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake and retention of radiolabeled estradiol by the uterus was examined in the armadillo. One pregnant and two non-pregnant armadillos were treated with 1.4 μg/kg body weight of 3H-estradiol (E2) by injection into the left ventricle, and one non-pregnant animal was injected with both the labeled hormone and 140 μg/kg body weight of unlabeled E2. One and a half hour after injection, the animals were sacrificed and the uteri were removed and processed for autoradiography. In the non-pregnant animals, nuclear localization was observed in the interstitial cells and glandular epithelium of the endometrium and the connective tissue cells and smooth muscle of the myometrium. Additionally, there was a gradation of uptake in the epithelial cells of the endometrium in that the glandular cells of the basal region were heavily labeled, while those cells in the sinusoidal, and luminal regions contained successively less label. The luminal cells were poorly labeled. In the pregnant female, the smooth muscle and glandular cells hypertrophied and their nuclei contained less label than was observed in the non-pregnant animals. The arteries of the myometrium were more easily distinguished in the pregnant animals and the nuclei of the endothelial cells and smooth muscle were more consistently labeled than those of the non-pregnant armadillos.
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    URL: http://dx.doi.org/10.1007/BF00210460
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  • 17
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    Electronic Resource
    Autoradiographic localization of 3H-GABA uptake in the thyroid gland of the rat (1981)
    Springer
    Cell & tissue research 220 (1981), S. 873-879 
    Details
    ISSN: 1432-0878
    Keywords: γ-Aminobutyric acid (GABA) ; Autoradiography ; Thyroid gland ; Transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake of γ-aminobutyric acid (GABA) in the thyroid gland of the rat was studied autoradiographically following in vitro incubation. High-affinity GABA uptake was localized in follicle cells, whereas C cells (parafollicular cells) in general did not accumulate GABA by high-affinity transport. The follicle cells were also the main sites of low-affinity GABA uptake. Additionally, some nerve fibres were found to accumulate GABA. The predominant localization of GABA uptake in follicle cells is discussed in view of a presumed role of GABA in thyroid function.
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    URL: http://dx.doi.org/10.1007/BF00210468
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  • 18
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    Autoradiographic studies with 3H 1,25 (OH)2 vitamin D3 and 3H 25 (OH) vitamin D3 in rat parathyroid glands (1981)
    Springer
    Cell & tissue research 221 (1981), S. 333-338 
    Details
    ISSN: 1432-0878
    Keywords: 1,25(OH)2 ; Vitamin D3 ; Receptors ; Parathyroid glands ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After injection of radiolabeled 1,25 (OH)2 vitamin D3, nuclear concentration of radioactivity is observed in parenchymal cells of the parathyroid gland in pregnant, adult male, and 10-day male neonatal rats. In competition studies with unlabeled 1,25 (OH)2 vitamin D3, but not with 25 (OH) vitamin D3, nuclear uptake is prevented. Experiments with 3H 25 (OH) vitamin D3, in contrast to 3H 1,25 (OH)2 vitamin D3, do not show nuclear concentration in cells of the parathyroid. The results of the autoradiographic studies suggest the presence of receptors for a direct effect of 1,25 (OH)2 vitamin D3 on the parathyroid gland for modulation of parathyroid hormone secretion.
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    URL: http://dx.doi.org/10.1007/BF00216737
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  • 19
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    Functions of digestive diverticula in marine invertebrates (1981)
    Springer
    Cell & tissue research 219 (1981), S. 185-195 
    Details
    ISSN: 1432-0878
    Keywords: Glucose ; Pyloric gland ; Ascidian ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The movement of labelled glucose along the digestive tract of two ascidian species, Sidnyum argus and Dendrodoa grossularia is compared. In Dendrodoa grossularia, glucose absorption takes place in all parts of the digestive tract except the oesophagus. Labelled glucose passes through the digestive tract in less than 12 h, and it accumulates in the pyloric ampullae in less than 3 days. In this species, the pyloric gland has more capacity to stock glucidic compounds than other tissues. Under normal feeding conditions, after five days, there is no transfer of labelled glucose to other tissues. Whatever the substances given to Sidnyum argus, its pyloric gland never accumulates labelled products.
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    URL: http://dx.doi.org/10.1007/BF00210027
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  • 20
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    Aspects of cellular proliferation during follicular atresia in the dog ovary (1981)
    Springer
    Cell & tissue research 219 (1981), S. 173-183 
    Details
    ISSN: 1432-0878
    Keywords: Ovary (dog) ; Atresia ; Thymidine ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Autoradiography after pulse labelling with [3H] thymidine was applied to investigate the proliferation processes in the granulosa and theca related to follicular atresia of the dog ovary during metestrus. The number of proliferating cells depends on the follicle type and its atretic stage. There is less proliferation in smaller secondary follicles than either in larger ones or tertiary follicles. While in early atresia tertiary follicles show the highest labelling indices, in advanced atresia the larger secondary follicles are those with the highest values. For each follicle type a decline in the labelling indices can be observed from early to terminal atresia. Tertiary follicles show a precipitous decrease in the labelling index between early and advanced atresia. There is a continuous gradient of proliferation from the center of the follicle over the peripheral granulosa to the theca. In tertiary follicles, an inverse correlation between labelling and necrosis of granulosa cells can be observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210026
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  • 21
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    Electronic Resource
    p-Chlorophenylalanine-induced proliferation of the seminal vesicle epithelium (1981)
    Springer
    Cell & tissue research 219 (1981), S. 159-172 
    Details
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Proliferation ; Autoradiography ; Biochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intraperitoneal injection of p-chlorophenylalanine (pCPA) methylester (100 mg/kg body weight) results in an activation of the lysosomal system of the secretory cells in the rat seminal vesicle and an elevation of the activities of lysosomal enzymes within 15 min following the injection. Large autophagic vacuoles are formed, sequestering rough endoplasmic reticulum and part of the Golgi apparatus within 2 h. Shortly after the activation of the lysosomal system an elevation of both DNA and protein synthesis is measured biochemically. 6 h subsequent to the injection a wave of mitoses of the secretory cells begins, reaching a maximum 6 h later and then declining within 3 h. About 12 h following the injection a second rise in lysosomal activity begins, declining within 24 h. The entire sequence of lysosomal and proliferative activities is inhibited in antiandrogen-pretreated rats. Deduced from these findings the following hypothesis of growth regulation of the accessory sex glands is advanced: enhanced loss of intracellular material during autophagocytosis diminishes the intracellular concentration of a substance curtailing cell division below its effective threshold resulting in division of the secretory cells. The prerequisites of this mechanism are (i) a sufficient distributive capacity of the stroma for hormones (androgens) and metabolic precursors, and (ii) sufficient capacity of the basal cells for transporting the precursors to the secretory cells. Sloughing of the secretory cells separates them from these auxiliary structures (stroma and basal cells) and enables the basal cells to divide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210025
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