ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (11,979)
  • Chemical Engineering  (11,634)
  • biodegradation  (208)
  • immobilization  (119)
  • Fisheries
  • Organic Chemistry
  • Process Engineering, Biotechnology, Nutrition Technology  (11,803)
  • Energy, Environment Protection, Nuclear Power Engineering  (176)
Collection
  • Articles  (11,979)
Keywords
Publisher
Years
  • 1
    ISSN: 1572-9729
    Keywords: biodegradation ; Burkholderia ; fenitrothion ; mpd gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A short rod shaped, gram-negative bacterium strain Burkholderia sp. FDS-1 was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. The isolate was capable of using fenitrothion as the sole carbon source for its growth. FDS-1 first hydrolyzed fenitrothion to 3-methyl-4-nitrophenol, which was further metabolized to nitrite and methylhydroquinone. The addition of other carbon source and omitting phosphorus source had little effect on the hydrolysis of fenitrothion. The gene encoding the organophosphorus hydrolytic enzyme was cloned and sequenced. The sequence was similar to mpd, a gene previously shown to encode a parathion-methyl-hydrolyzing enzyme in Plesiomonas sp. M6. The inoculation of strain FDS-1 (106 cells g−1) to soil treated with 100 mg fenitrothion emulsion kg−1 resulted in a higher degradation rate than in noninoculated soils regardless of the soil sterilized or nonsterilized. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 17 (2006), S. 207-217 
    ISSN: 1572-9729
    Keywords: biodegradation ; DGGE ; K2Ni(CN)4 soil bacterial populations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Metal cyanides are significant contaminants of many soils found at the site of former industrial activity. In this study we isolated bacteria capable of degrading ferric ferrocyanide and K2Ni(CN)4. One of these bacteria a Rhodococcus spp. was subsequently used to bioaugment a minimal medium broth, spiked with K2Ni(CN)4, containing 1 g of either an uncontaminated topsoil or a former coke works site soil. Degradation of the K2Ni(CN)4 was observed in both soils, however, bioaugmentation did not significantly impact the rate or degree of K2Ni(CN)4 removal. Statistical analysis of denaturing gradient gel electrophoresis profiles showed that the topsoil bacterial community had a higher biodiversity, and its structure was not significantly affected by either K2Ni(CN)4 or bioaugmentation. In contrast, profiles from the coke works site indicated significant changes in the bacterial community in response to these additions. Moreover, in both soils although bioaugmentation did not affect rates of biodegradation the Rhodococcus spp. did become established in the communities in broths containing both top and coke works soil. We conclude that bacterial communities from contaminated soils with low biodiversity are much more readily perturbed through interventions such as contamination events or bioaugmentation treatments and discuss the implications of these findings for bioremediation studies.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1572-9729
    Keywords: 16S rDNA ; biodegradation ; Biolog ; Klebsiella ; thiocyanate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A mixed bacterial culture capable of growing in potassium-thiocyanatecontaining medium (200 mg KSCN) has been isolated from bacterial suspensions of soilsamples collected near gold mines in Kumjung (Korea). The isolates were initially characterized by metabolic profile analysis and were identified as Bacillus thermoglucosidasius,Bacillus cereus, Bacillus licheniformis, Bacillus mycoides, Brevibacteriumepidermidis, Brevibacterium otitidis, and Corynebacterium nitrilophilus.One of the seven isolates was initially characterized as Brevibacterium epidermidis,which is not known to degrade thiocyanate. However, using 16S rDNA sequencing, thisstrain was identified as a member of Klebsiella. The strain shows high similarityvalues (95.8 to 96.4%) with Klebsiella species, and the closest known relative was foundto be K. ornithinolytica ATCC 31898. The result indicates that species of the genusKlebsiella were the closest phylogenetic relatives of the investigated strain. This isthe first known report of a member of Klebsiella that is capable of utilizing thiocyanate assole source of carbon and nitrogen.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 8 (2000), S. 67-79 
    ISSN: 1572-8900
    Keywords: Polyvinyl alcohol ; biodegradation ; soil, clay ; adsorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The rates and extents of absorption and desorption of polyvinyl alcohol (PVA) samples on different solid substrates comprising montmorillonite, quartz sand, and farm soil, as well as humic acid mixture are reported. The uptake of PVA by the substrates was analyzed as a function of PVA hydrolysis (72–98%), molecular weight, and molecular weight distribution. Higher adsorption was detected on montmorillonite followed by farm soil, whereas the quartz sand did not display any specific adsorption affinity for the PVA. An increase in the hydrolysis degree of PVA increased the adsorption rate and extent whereas this feature was reversed by an increase in PVA molecular weight. The desorption of PVA samples from the different substrates was performed both under various pH conditions and in the presence of concentrated HF that was used to dissolve the silicon derivatives present in the substrates. Biodegradation experiments carried out in liquid cultures of PVA adsorbed on montmorillonite showed that the mineralization of the adsorbed PVA was much lower than that detected for the nonadsorbed PVA. This investigation suggests that irreversible adsorption of PVA on the clay component occurs in soil, thus substantially inhibiting PVA biodegradation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 8 (2000), S. 91-96 
    ISSN: 1572-8900
    Keywords: Polyester amide ; hemp fiber ; composite ; biodegradation ; carbon balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In a composite, fast degradable fibers determine the degradation of the slowly degradable matrix. Such biodegradable composites consisting of degummed hemp fibers and a polyester amide matrix were produced with fiber mass fractions between 0 and 0.48. The hot-pressed plates, 1-mm thick, were incubated in a standard soil. The degradation kinetics was quantified by the measurement of CO2 production. Furthermore, after termination of experiment, the carbon balance was uncovered. The results were fitted to an exponential law taking into account the degradation of fibers. The increased amount of pores realized by high fiber contents induces pronounced degradation. The degradation is fully characterized by the time constant τ, which is correlated to the fiber mass fraction. The model allows to predict the degradation kinetics of composites with a few well-defined experiments.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 8 (2000), S. 167-174 
    ISSN: 1572-8900
    Keywords: Polyimides ; electronic insulation ; biodegradation ; biodeterioration ; fungi ; electrochemical impedance spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An electrochemical impedance spectroscopy (EIS) technique was evaluated for monitoring microbial degradation of electronic packaging polyimides. The microbial inoculum was a mixed culture of fungi isolated previously from deteriorated polyimides. The active fungal consortium comprised Aspergillus versicolor, Cladosporium cladosporioides, and a Chaetomium species. After inoculation, fungal growth on the polyimides resulted in distinctive EIS spectra indicative of polymer insulation failure, which directly related to polymer integrity. Degradation appeared to occur in a number of steps and two distinctive stages in the decline of film resistance were observed in the inoculated EIS cells within the 2 and 10 weeks after inoculation. The early stage of resistance decrease may be related to the ingress of water molecules and ionic species into the polymeric materials, whereas the second stage probably resulted from partial degradation of the polymers by fungal growth on the polymer film. The relationship between changes of impedance spectra and microbial degradation of the polymer was further supported by scanning electron microscopy (SEM) observations of fungi growing on the surface of the inoculated polyimides. Our data indicate that the EIS can be used in detection of early degradation of resistant polymers and polyimides that are susceptible to biodeterioration.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1572-8900
    Keywords: Extruded ; polyvinyl alcohol ; starch ; glycerol ; biodegradation ; films
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Elongation properties of extruded cornstarch were improved by blending with glycerol. Further blending of starch-glycerol with polyvinyl alcohol (PVOH) resulted in significant improvements in both tensile strength (TS) and elongation at break. Samples of starch-glycerol without PVOH equilibrated at 50% relative humidity had a TS of 1.8 MPa and elongation of 113%, whereas those containing PVOH had a TS and elongation of 4 MPa and 150%, respectively. Dynamic mechanical analysis (DMA) of starch-glycerol-PVOH blends showed that decreases in glass transition temperatures (T g values) were proportional to glycerol content. Scanning electron microscopy (SEM) of fractured surfaces revealed numerous cracks in starch-glycerol (80:20) samples. Cracks were absent in starch-glycerol (70:30) samples. In both blends, many starch granules were exposed at the surface. No exposed starch granules were visible in blends with added PVOH. Starch-glycerol samples incubated in compost lost up to 70% of their dry weight within 22 days. Addition of PVOH lowered both the rate and extent of biodegradation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1572-8900
    Keywords: Polyion complex fibers ; biodegradation ; soil filamentous fungi, chitosan ; poly(L-lysine) ; gellan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The degradation of two kinds of polyion complex (PIC) fibers, chitosan-gellan (CGF), and poly(L-lysine)-gellan (LGF) fibers, by seven species of soil filamentous fungi has been investigated. All of the pure-line soil filamentous fungi, Aspergillus oryzae, Penicillium caseicolum, P. citrinum, Mucor sp., Rhizopus sp., Curvularia sp., and Cladosporium sp. grew on the two fiber materials. Microscopic observation of the biodegradation processes revealed that P. caseicolum on the CGF and LGF grew, along with the accompanying collapse of the fiber matrices. In the biochemical oxygen-demand (BOD) test, the biodegradation of the LGF by P. caseicolum and Curvularia sp. exceeded 97% carbon dioxide generation and the biodegradation of the CGF by A. oryzae was 59%. These results might offer some clues to the applications of the PIC fibers as environmentally biodegradable materials.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 11 (2000), S. 289-294 
    ISSN: 1572-9729
    Keywords: biodegradation ; cellular fatty acids ; crude oil ; end products ; hydrocarbons ; rhodococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A hydrocarbon degrader isolated from a chronically oil-polluted marine site was identified as Rhodococcus sp. on the basis of morphology, fatty acid methyl ester pattern, cell wall analysis, biochemical tests and G + C content of DNA. It degraded upto 50% of the aliphatic fraction of Assam crude oil, in seawater supplemented with 35 mM nitrogen as urea and 0.1 mM phosphorus as dipotassium hydrogen orthophosphate, after 72 h at 30 ° and 150 revolutions per minute. The relative percentage of intracellular fatty acid was higher in hydrocarbon-grown cells compared to fructose-grown cells. The fatty acids C16 , C1616 :1 C18 and C18 : 1 were constitutively present regardless of the growth substrate. In addition to these constitutive acids, other intracellular fatty acids varied in correlation to the hydrocarbon chain length supplied as a substrate. When grown on odd carbon number alkanes, the isolate released only monocarboxylic acids into the growth medium. On even carbon number alkanes only dicarboxylic acids were produced.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 11 (2000), S. 323-329 
    ISSN: 1572-9729
    Keywords: biodegradation ; microbial polyester ; poly(3-hydroxybutyrate) ; poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Poly(3-hydroxybutyrate) [P(3HB)] test-pieces prepared from the polymer produced by Azotobacter chroococcum were degraded in natural environments like soil, water, compost and sewage sludge incubated under laboratory conditions. Degradation in terms of % weight loss of the polymer was maximum (45%) in sewage sludge after 200 days of incubation at 30°C. The P(3HB)-degrading bacterial cultures (36) isolated from degraded test-pieces showed different degrees of degradation in polymer overlayer method. The extent of P(3HB) degradation increases up to 12 days of incubation and was maximum at 30°C for majority of the cultures. For most efficient cultures the optimum concentration of P(3HB) for degradation was 0.3% (w/v). Supplementation of soluble carbon sources like glucose, fructose and arabinose reduced the degradation while it was almost unaffected with lactose. Though the cultures degraded P(3HB) significantly, they were comparatively less efficient in utilizing copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate [P(3HB-co-3HV)].
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 11
    ISSN: 1572-9729
    Keywords: biodegradation ; biofiltration ; hydrogen sulfide ; malodorous compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A laboratory scale bioreactor has been designed and set up in order to degrade hydrogen sulfide from an air stream. The reactor is a vertical column of 7 litre capacity and 1 meter in height. It is divided into three modules and each module is filled with pellets of agricultural residues as packing bed material. The gas stream fed into the reactor through the upper inlet consists of a mixture of hydrogen sulfide and humidified air. The hydrogen sulfide content in the inlet gas stream was increased in stages until the degradation efficiency was below 90%. The parameters to be controlled in order to reach continuous and stable operation were temperature, moisture content and the percentage of the compound to be degraded at the inlet and outlet gas streams (removal or elimination efficiency). When the H2S mass loading rate was between 10 and 40 g m-3h-1, the removal efficiency was greater than 90%. The support material had a good physical performance throughout operation time, which is evidence that this material is suitable for biofiltration purposes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 12
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 151-154 
    ISSN: 1573-0972
    Keywords: Invertases ; immobilization ; phenyl-Sepharose ; thermophilic fungus ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An invertase from the thermophilic fungus, Thermomyces lanuginosus was immobilized on phenyl-Sepharose and its properties were studied. Between the soluble and immobilized forms of the invertase, there were not much difference in their optimum pH, K M and V max for sucrose. In contrast, the K M and V max for raffinose changed significantly. The optimum temperature for the immobilized invertase was lower by 10 ∘C. The immobilized invertase showed remarkable stability at 50 ∘C and was less sensitive to inhibition by metal ions. There was no leaching of the enzyme for at least a month when stored in the refrigerator. The method is novel and specific for the thermophilic invertase as a mesophilic invertase (from yeast) did not bind to phenyl-Sepharose.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 13
    ISSN: 1572-9729
    Keywords: aromatic hydrocarbons ; biodegradation ; CEC L-33-A-93 test ; lubricants ; mineral base oils ; viscosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The primary biodegradability of two types of paraffinic base oils (solvent and catalytically dewaxed oils) and their blends was evaluated using the CEC L-33-A-93 test. The biodegradability values varied between 10% and 75%. Base oil mixtures displayed varying contents in aromatic and polar compounds and a wide range of kinematic viscosity (KV) values, from roughly 10 to 600 cSt (at 40°C), while their viscosity indices were almost constant (90-100). The biodegradability of oils was closely related to their content in polycyclic aromatic hydrocarbons and was also decreasing with kinematic viscosity. For the two types of base oils, a linear relationship could be set between the biodegradation percentages and the logarithms of KV values. These results show that, beside overall chemical features such as the contents in aromatic compounds, KV may be a prominent parameter for assessing the primary biodegradability of mineral base oils.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 11 (2000), S. 239-246 
    ISSN: 1572-9729
    Keywords: 1,4-Dioxane ; biodegradation ; cometabolism ; ethers ; tetrahydrofuran
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A mixed culture with the ability to aerobically biodegrade 1,4-dioxane in the presence of tetrahydrofuran (THF) was enriched from a 1,4-dioxane contaminated aquifer. This consortium contained 3–4 morphologically different types of colonies and was grown in mineral salts media. Biodegradation of 1,4- dioxane began when THF concentrations in batch experiments became relatively low. No biodegradation of 1,4-dioxane was observed in the absence of THF and the measured cell yield was similar during degradation of 1,4-dioxane with THF or with THF alone. However, when the consortium was grown in the presence of 14C-1,4-dioxane plus THF, 2.1% of the radiolabeled 1,4-dioxane was present in the particulate fraction. The majority of the 14C (78.1%) was recovered as 14CO2, while 5.8% remained in the liquid fraction. This activity is interesting since the non-growth substrate is mineralized, yet only minimally assimilated into biomass. Using THF as the growth substrate, 1,3-dioxane, methyl t-butyl ether, ethyl t-butyl ether and t-amyl methyl ether.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 15
    ISSN: 1572-9729
    Keywords: biodegradation ; gasoline ; hydrocarbon ; intrinsic capacities ; natural attenuation ; soil pollution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A methodology allowing the detailed assessment ofthe capacities of microflorae to degrade gasoline inaerobic conditions has been developed. It consisted inthe determination of the degradation of a gasolinemodel mixture in liquid cultures in optimalconditions. The gasoline model mixture contained 23representative hydrocarbons of gasoline (GM23). Thekinetics and extent of biodegradation were evaluatedby continuous overall monitoring of CO2production and final chromatographic analysis (usuallyafter about 30 days) of the consumption of eachhydrocarbon. The methodology was used with soil andwater samples from polluted and non polluted sites.The experimentation aimed at assessing thedistribution of the degradative capacities in theenvironment and the prospects for natural attenuationof gasoline. Nine microflorae were tested. The intrinsicbiodegradability (existence of mechanisms ofbiodegradation) appeared total for GM23 as shown bythe results obtained with several microflorae. Thedegradative capacities of microflorae from nonpolluted samples were high (total degradation rates atleast 85%). Incomplete degradation was observedessentially for trimethylalkanes(2,2,4-trimethylpentane and 2,3,4-trimethylpentane)and for cyclohexane. In several cases, samples frompolluted sites exhibited more extensive degradativecapacities, with total degradation of all hydrocarbonsbeing observed for three out of the six samples.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 16
    ISSN: 1572-9729
    Keywords: Biodegradation ; immobilization ; Nocardioides ; phenol ; p-nitrophenol ; simultaneous degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of the presence of an alternate toxiccompound (phenol) on the p-nitrophenol(PNP)-degrading activity of freely suspended andcalcium alginate immobilized Nocardioides sp.NSP41 was investigated. In the single substrateexperiments, when the concentration of phenol and PNPwas increased to 1400 mg l-1 and 400 mg l-1,respectively, the initial cell concentrations in thefreely suspended cell culture should be higher than1.5 g dry cell weight l-1 for completedegradation. In the simultaneous degradationexperiment, when the initial concentration of phenolwas increased from 100 to 400 mg l-1, thespecific PNP degradation rate at the concentration of200 mg l-1 was decreased from 0.028 to 0.021h-1. A freely suspended cell culture with a highinitial cell concentration resulted in a highvolumetric degradation rate, suggesting the potentialuse of immobilized cells for simultaneous degradation.In the immobilized cell cultures, althoughsimultaneous degradation of PNP and phenol wasmaintained, the specific PNP and phenol degradationrate decreased. However, a high volumetric PNP andphenol degradation rate could be achieved byimmobilization because of the high cell concentration.Furthermore, when the immobilized cells were reused inthe simultaneous degradation of PNP and phenol, theydid not lose their PNP- and phenol-degrading activityfor 12 times in semi-continuous cultures. Takentogether, the use of immobilized Nocardioidessp. NSP41 for the simultaneous degradation of PNP andphenol at high concentrations is quite feasiblebecause of the high volumetric PNP and phenoldegradation rate and the reusability of immobilizedcells.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 11 (2000), S. 55-63 
    ISSN: 1572-9729
    Keywords: Alcaligenes defragrans ; biodegradation ; essential oil ; monoterpenes ; nitrate reduction ; plant ; volatile organic compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant volatile organic compounds are a major carbonsource in nature. We studied the degradability ofthese substances by anaerobic microorganisms inenrichment cultures with representative essential oilsas organic substrates and nitrate as electronacceptor. Lemon and pine needle oil supportedmicrobial growth in the presence of pure oil, whereasparsley seed, camphor, sage, fennel, and mint oilsupported growth only when the essential oils weredissolved in an overlying phase of2,2,4,4,6,8,8-heptamethylnonane. Thyme oil did notsupport denitrification. Analyses of the microbiallydegraded oils revealed the disappearance ofmonoterpenes, of several monoterpenoids, and ofmethoxy-propenyl-benzenes, including apiole andmyristicin. Most-probable-number determinations fordenitrifying communities in sewage sludge and forestsoil yielded 106 to 107monoterpene-utilizing cells ml-1, representing0.7 to 100% of the total cultivablenitrate-reducing microorganisms. The utilization ofessential oils together with the common occurrence ofthis metabolic trait are indications for anenvironmentally important, but currently unexploredanaerobic turnover of plant volatile organic compoundsin soil.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 18
    ISSN: 1572-9729
    Keywords: Availability ; Bacillus ; biodegradation ; biosurfactant ; hydrocarbon ; surfactin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A non-sterile biosurfactant preparation (surfactin)was obtained from a 24-h culture of Bacillussubtilis O9 grown on sucrose and used to study itseffect on the biodegradation of hydrocarbon wastes byan indigenous microbial community at theErlenmeyer-flask scale. Crude biosurfactant was addedto the cultures to obtain concentrations above andbelow the critical micelle concentration (CMC). Lowerconcentration affected neither biodegradation normicrobial growth. Higher concentration gave highercell concentrations. Biodegradation of aliphatichydrocarbons increased from 20.9 to 35.5% and in thecase of aromatic hydrocarbons from nil to 41%,compared to the culture without biosurfactant. Theenhancement effect of biosurfactant addition was morenoticeable in the case of long chain alkanes. Pristaneand phytane isoprenoids were degraded to the sameextent as n-C17 and n-C18 alkanes and, consequently,no decrease in the ratios n-C17/pri and n-C18/phy wasobserved. Rapid production of surfactin crudepreparation could make it practical for bioremediationof ship bilge wastes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 19
    ISSN: 1572-9729
    Keywords: anaerobic ; biodegradation ; BTEX ; fuel ; hydrocarbons ; Seal Beach
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Enhanced anaerobic biodegradation of groundwater contaminated by fuel hydrocarbons has been evaluated at a field experiment conducted at the Naval Weapons Station, Seal Beach, California. This experiment included the establishment of three different remediation zones in situ: one zone was augmented with sulfate, one was augmented with sulfate and nitrate, and the third was unaugmented. This enables a comparison of hydrocarbon biodegradation under sulfate-reducing, sequential denitrifying/sulfate-reducing, and methanogenic conditions, respectively. In general, the results from the field experiment are: (1) Certain fuel hydrocarbons were removed preferentially over others, but the order of preference is dependent upon the geochemical conditions; and (2) In the zones that were augmented with sulfate and/or nitrate, the added electron acceptors were consumed quickly, indicating that enhancement via electron acceptor injection accelerates the biodegradation process. More specifically, in the sulfate-reducing zone, sulfate was utilized with an apparent first-order rate coefficient of approximately 0.1 day-1. In the combined denitrifying/sulfate-reducing zone, nitrate was utilized preferentially over sulfate, with an apparent first-order rate coefficient of 0.1–0.6 day-1. However, the data suggest that slow sulfate utilization does occur in the presence of nitrate, i.e., the two processes are not strictly sequential. With regard to the aromatic BTEX hydrocarbons, toluene was preferentially removed under intrinsic conditions; biodegradation of benzene was slow if it occurred at all; augmentation with sulfate preferentially stimulated biodegradation of o-xylene; and ethylbenzene appeared recalcitrant under sulfate-reducing conditions but readily degradable under denitrifying conditions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 20
    ISSN: 1572-9729
    Keywords: aerobic ; bioremediation ; biodegradation ; gasoline ; MTBE ; TBA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With the current practice of amending gasoline with up to 15% by volume MTBE, the contamination of groundwater by MTBE has become widespread. As a result, the bioremediation of MTBE-impacted aquifers has become an active area of research. A review of the current literature on the aerobic biodegradation of MTBE reveals that a number of cultures from diverse environments can either partially degrade or completely mineralize MTBE. MTBE is either utilized as a sole carbon and energy source or is degraded cometabolically by cultures grown on alkanes. Reported degradation rates range from 0.3 to 50 mg MTBE/g cells/h while growth rates (0.01–0.05 g MTBE/g cells/d) and cellular yields (0.1–0.2 g cells/g MTBE) are generally low. Studies on the mechanisms of MTBE degradation indicate that a monooxygenase enzyme cleaves the ether bond yielding tert-butyl alcohol (TBA) and formaldehyde as the dominant detectable intermediates. TBA is further degraded to 2-methyl-2-hydroxy-1-propanol, 2-hydroxyisobutyric acid, 2-propanol, acetone, hydroxyacteone and eventually, carbon dioxide. The majority of these intermediates are also common to mammalian MTBE metabolism. Laboratory studies on the degradation of MTBE in the presence of gasoline aromatics reveal that while degradation rates of other gasoline components are generally not inhibited by MTBE, MTBE degradation could be inhibited in the presence of more easily biodegradable compounds. Controlled field studies are clearly needed to elucidate MTBE degradation potential in co-contaminant plumes. Based on the reviewed studies, it is likely that a bioremediation strategy involving direct metabolism, cometabolism, bioaugmentation, or some combination thereof, could be applied as a feasible and cost-effective treatment method for MTBE contamination.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 21
    Electronic Resource
    Electronic Resource
    Springer
    Environment, development and sustainability 2 (2000), S. 277-304 
    ISSN: 1573-2975
    Keywords: Systems of Knowledge ; Local Knowledge ; Fisheries ; Resource Management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Sociology
    Notes: Abstract During the last 20 years, the existence of rich systems of local knowledge, and their vital support to resource use and management regimes, has been demonstrated in a wide range of biological, physical and geographical domains, such as agriculture, animal husbandry, forestry and agroforestry, medicine, and marine science and fisheries. Local knowledge includes empirical and practical components that are fundamental to sustainable resource management. Among coastal-marine fishers, for example, regular catches and, often, long-term resource sustainment are ensured through the application of knowledge that encompasses empirical information on fish behaviour, marine physical environments, fish habitats and the interactions among ecosystem components, as well as complex fish taxonomies. Local knowledge is therefore an important cultural resource that guides and sustains the operation of customary management systems. The sets of rules that compose a fisheries management system derive directly from local concepts and knowledge of the resources on which the fishery is based. Beyond the practical and the empirical, it is essential to recognise the fundamental socio-cultural importance of local knowledge to any society. It is through knowledge transmission and socialisation that worldviews are constructed, social institutions perpetuated, customary practices established, and social roles defined. In this manner, local knowledge and its transmission, shape society and culture, and culture and society shape knowledge. Local knowledge is of great potential practical value. It can provide an important information base for local resources management, especially in the tropics, where conventionally-used data are usually scarce to non-existent, as well as providing a shortcut to pinpoint essential scientific research needs. To be useful for resources management, however, it must be systematically collected and scientifically verified, before being blended with complementary information derived from Western-based sciences. But local knowledge should not be looked on with only a short-term utilitarian eye. Arguments widely accepted for conserving biodiversity, for example, are also applicable to the intellectual cultural diversity encompassed in local knowledge systems: they should be conserved because their utility may only be revealed at some later date or owing to their intrinsic value as part of the world's global heritage. At least in cultures with a Western liberal tradition, more than lip-service is now being paid to alternative systems of knowledge. The denigration of alternative knowledge systems as backward, inefficient, inferior, and founded on myth and ignorance has recently begun to change. Many such practices are a logical, sophisticated and often still-evolving adaptation to risk, based on generations of empirical experience and arranged according to principles, philosophies and institutions that are radically different from those prevailing in Western scientific circles, and hence all-but incomprehensible to them. But steadfastly held prejudices remain powerful. In this presentation I describe the 'design principles' of local knowledge systems, with particular reference to coastal-marine fishing communities, and their social and practical usefulness. I then examine the economic, ideological and institutional factors that combine to perpetuate the marginalisation and neglect of local knowledge, and discuss some of the requirements for applying local knowledge in modern management.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Environmental monitoring and assessment 60 (2000), S. 251-260 
    ISSN: 1573-2959
    Keywords: BOD ; immobilization ; microbial consortium ; ready-to-use seeding material ; reusable ; calcium alginate beads
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A number of microorganisms were isolated from sewage. Biochemical Oxygen Demand (BOD-5 day) analysis was carried out by individual pure cultures. The cultures giving higher or equal BOD values as compared to reference GGA solution were selected for the formulation of a defined mixed microbial consortium. This microbial consortium was co-immobilized on calcium-alginate beads. Four synthetic and six industrial samples were tested for BOD by using immobilized beads as well as sewage as source of seeding materials. BOD values obtained with beads for all the synthetic as well as industrial samples were fairly comparable with those obtained with sewage. Reusability of prepared microbial beads was also checked with different synthetic and industrial samples and was compared with reference GGA solution. The same microbial beads can be reused three times for different BOD-5 day estimations. It is recommended that immobilized microbial beads can be used as a ready-to-use seeding material for BOD analysis.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 23
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 123 (2000), S. 133-146 
    ISSN: 1573-2932
    Keywords: recalcitrat organics ; biodegradation ; biofiltration ; methyl tert-butyl ether ; dichloromethane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Biodegradation of two recalcitrant compounds, dichloromethane and methyl tert-butyl ether was investigated individually and in combination with toluene and benzene. A vapor phase biofilter operating at an air flux of 1 m3/m2·min and an empty bed residence time of 1 minute was used as the reaction system. Inlet recalcitrant contaminant concentrations were typically 35 ppm on a volume basis while the aromatic compound concentrations were varied from 8 ppm to 150 ppm. Dichloromethane removals were not impacted by the introduction of toluene. However, a rapid decrease in methyl tert-butyl ether removal resulted from the initial introduction of toluene. Complete removal of both methyl tert-butyl ether and toluene was achieved within a week of operation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 24
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 245-248 
    ISSN: 1573-0972
    Keywords: Acetobacter xylinum ; biotechnology ; carbon source ; cellulose membrane ; immobilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of various carbon and nitrogen sources on cellulose membrane production by Acetobacter xylinum was evaluated. Among the carbon sources, sucrose, glucose and mannitol were found to be suitable for optimum levels of cellulose production. The strain was able to utilize a wide range of protein and nitrogen sources such as peptone, soybean meal, glycine, casein hydrolysate, and glutamic acid for cellulose synthesis. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of pellicle proteins (PP) revealed electrophoretic bands of molecular masses in the range of 116–20 kDa. Furthermore, the strain can be useful for the removal of various nitrogenous and carbon substrates present in waste waters.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 25
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 393-395 
    ISSN: 1573-0972
    Keywords: Aerobic bacteria ; biodegradation ; nitroaromatic compounds ; 2,4,6-trinitrotoluene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Several strains of aerobic bacteria tolerant to some chemical toxins were isolated from the water of man-made Yerevan Lake and from the soil and air of different regions of the city of Yerevan. Some of these bacteria were capable of degrading 2,4,6-trinitrotoluene (TNT) during shake-flask fermentation in liquid medium. Two bacterial strains with good ability to degrade TNT were isolated. These strains showed visible morphological and physiological differences during growth on numerous elective media. The comparative ability of these strains to transform TNT was investigated.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 26
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 95-98 
    ISSN: 1573-0972
    Keywords: Anaerobic ; aerobic ; bioreactors ; biodegradation ; chlorophenols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Chlorophenol degradation was studied by combined anaerobic–aerobic treatments as a single or multi-substrate system. 2,4-Dichlorophenol (2,4-DCP) was degraded to the extent of 52 and 78% in up-flow anaerobic sludge blanket (UASB) and aerobic suspended growth (ASG) reactors respectively, at organic loading rates of 0.18 kg/m3/day and hydraulic retention time of 26.4 h in the presence of glucose. The UASB represents the dominating facultative anaerobic microbial population. When the effluent from the anaerobic reactor (UASB) was subjected to aerobic treatment on the ASG reactor, 2,4-DCP and COD removals of 86 and 95% respectively were achieved. Aerobic degradation of chlorophenol by acclimated mixed bacterial isolates was found to be sequential: 2-Chlorophenol (2-CP) and 4-CP were degraded first, followed by 2,4-DCP and 2,4,6-Trichlorophenol (2,4,6-TCP) while the contrary was obtained in anaerobic degradation. In anaerobic degradation by acclimated mixed bacterial cells, 2,4-DCP and 2,4,6-TCP were degraded first followed by mono-chlorophenols. The anaerobic/aerobic bioreactors were most efficient when operated in sequence (series) rather than in parallel.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 27
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 495-497 
    ISSN: 1573-0972
    Keywords: Bacteria ; biodegradation ; 2,4-D ; soil microcosm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Bacterial isolates (NJ 10 and NJ 15) capable of degrading the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were isolated from agricultural soil by enrichment culture technique. The isolates exhibited substantial growth in mineral salt medium supplemented with 0.1–0.5% of 2,4-D as a sole source of carbon and energy. Based on their morphological, cultural and biochemical characteristics, the isolates NJ 10 and NJ 15 have been identified as Pseudomonas species and Pseudomonas aeruginosa, respectively. Biodegradation studies in a soil microcosm enriched with pure cultures of the isolates demonstrated a time-dependent disappearance of 2,4-D from the 100 mg/kg herbicide-amended soil. The HPLC data analysis revealed 96.6 and 99.8% degradation in the soil inoculated with the pure cultures of isolates NJ 10 and NJ 15, respectively with in 20 days of incubation at 30 °C. Both the isolates showed significant solubilization of inorganic phosphate [Ca3(PO4)2] on the specific Pikovskaya's medium.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 28
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 625-630 
    ISSN: 1573-0972
    Keywords: Aeromonas ; biodegradation ; protease ; psychrotrophic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Among 67 psychrotrophic bacterial isolates of Leh, India screened for production of hydrolytic enzymes at 10 °C, four belonging to Aeromonas hydrophila were characterized and evaluated for biodegradation of night soil. All strains produced metalloproteases on a variety of carbon and nitrogen sources. Strains LA1 and LA15 also produced α-amylase and PC5 both α- & β-amylase. No amylase was produced by PN7, however it produced lipase. Casein and glucose induced maximum enzyme activity (protease and amylase) in LA15 and PC5, respectively. In LA1, maximum induction of protease was observed with casein and of amylase with maltose. Corn oil/tributyrin served as the best inducers for protease and lipase production by PN7. A. hydrophila strains were found to be psychrotrophic with optimum growth and enzyme activity at 20 and 37 °C, respectively. Maximum biodegradation of night soil was observed by strain LA1 at 5–20 °C.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 29
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 7 (1999), S. 109-116 
    ISSN: 1572-8900
    Keywords: Kinetics ; aerobic ; biodegradation ; poly-3-hydroxybutyrate ; poly-ɛ-caprolactone ; ASTM D 5988-96
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The standard test method ASTM D 5988-96 for determining the degree and rate of aerobic biodegradation of plastic materials in contact with soil was applied to poly(3-hydroxybutyrate) and poly(ɛ-caprolactone). The method proved to be reliable and supplied reproducible measurements of CO2 production, provided potassium (instead of barium) hydroxide was used as a trapping solution. The trends of CO2 evolution, as a function of time, observed for the degradation of polymer powders in soil are similar to those predicted by simple first order kinetics in solution. The experimental data are described by a Michaelis–Menten type model, which accounts for the heterogeneity of the polymer-soil system. The kinetic equation deduced predicts the degradation rate to the proportional to the exposed polymer surface area.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 30
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 7 (1999), S. 35-40 
    ISSN: 1572-8900
    Keywords: Starch ; starch blends ; sorption ; diffusion ; biodegradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The interval sorption and diffusion of water vapor were studied for two systems: methylcellulose (MC)/starch and carboxymethylcellulose (CMC)/starch. The diffusion coefficient of water vapor and the Gibbs free energy of swelling of these blends in water were estimated. The Gibbs free energy of mixing starch with the cellulose derivatives was determined using the thermodynamic cycle. CMC/starch was shown to be more compatible than MC/starch. Biodegradation of these systems in the water–soil environment was measured and found to increase with the concentration of starch in its blends with cellulose derivatives.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 31
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 7 (1999), S. 19-26 
    ISSN: 1572-8900
    Keywords: Poly(tetramethylene succinate) ; amorphous and crystalline orientation ; biodegradation ; morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The effect of orientation in the amorphous and crystalline regions on the biodegradability of PTMS [poly(tetramethylene succinate)] was studied using the amorphous orientation function, birefringence, and crystallinity. The crystalline and amorphous intrinsic lateral sonic moduli, E t,c 0 and E t,am 0 , were 2.61 × 103 and 0.41 × 103 MPa, respectively. Using the data on birefringence, crystalline and amorphous orientation function (f ∈ and f am), crystallinity, and sonic modulus of the oriented PTMS fibers, the intrinsic birefringence of the crystalline (Δ c 0 ) and amorphous (Δ am 0 ) regions were evaluated to be 0.0561 and 0.0634, respectively. The biodegradabilities of oriented PTMS films were reduced as the elongation increased, i.e., the amorphous orientation increased. At low elongation (100 and 150%), however, biodegradabilities remained unchanged when the degradation test was performed in activated sludge, which was attributed to the amorphous orientation occurring even at 100% elongation, though the amorphous orientation direction was perpendicular to the fiber axis.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 32
    Electronic Resource
    Electronic Resource
    Springer
    Environmental monitoring and assessment 59 (1999), S. 257-274 
    ISSN: 1573-2959
    Keywords: biodegradation ; groundwater geochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Biotransformation processes play an active role in reducing the environmental impact of fuel hydrocarbon releases to groundwater. Because monitoring data at release locations are typically sparse, spatial variations in geochemical indicator parameters are often called upon as indirect evidence of biotransformation. These parameters include concentrations of electron acceptors (O2, NO3 -, SO〉 4 2- , reduced redox reaction by-products (Fe2+, Mn2+, CH4), as well as bicarbonate alkalinity, pH and Eh. However, background variability in a number of these parameters complicates the task of data interpretation, particularly in the case of small data sets. In this study, correlation analyses are applied to geochemical indicator data at six hydrocarbon groundwater contamination sites in California to identify which parameters are the most reliable indicators. The results of the analyses suggest that the most direct indicators of the local redox environment – Fe2+, Mn2+, CH4, Eh – yield the most consistent evidence of hydrocarbon biotransformation. Indicators which rely largely on mass balance – O2, NO 3 - , SO 4 2- , alkalinity – appear to be less reliable. These findings may provide guidance in both the collection and interpretation of groundwater monitoring data at hydrocarbon contamination sites.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 33
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 465-469 
    ISSN: 1573-0972
    Keywords: Biotransformation ; codeine ; immobilization ; morphine ; Spirulina platensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Both freely suspended cells and immobilized cultures of Spirulina platensis, a blue-green alga, biotransformed exogenously fed codeine, an opium alkaloid, to morphine. The external addition of codeine to the culture medium did not affect the growth of S. platensis. Immobilization of Spirulina in a calcium alginate gel matrix was optimized by using 2% (w/v) sodium alginate and reducing the concentration of nutrients of Zarrouk's medium, which caused destabilization of the calcium alginate gel. The accumulation of morphine increased gradually and reached maxima of 330 μg 100 ml−1 culture at 105 h in freely suspended and 351 μg 100 ml−1 at 96 h in immobilized Spirulina cultures. Accumulation of morphine was detected only in the medium, whereas cells did not show accumulation. The immobilized Spirulina cultures showed marginally higher conversion of codeine to morphine over freely suspended cultures.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 34
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 10 (1999), S. 15-25 
    ISSN: 1572-9729
    Keywords: anaerobic ; biodegradation ; BTEX ; gasoline ; hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We examined the extent of biodegradation of benzene, toluene, ethylbenzene and the three isomers of xylene (BTEX) as a mixture and from gasoline in four different sediments: the New York/New Jersey Harbor estuary (polluted); Tuckerton, N.J. (pristine); Onondaga Lake, N.Y. (polluted) and Blue Mtn. Lake, N.Y. (pristine). Enrichment cultures were established with each sediment using denitrifying, sulfidogenic, methanogenic and iron reducing media, as well as site water. BTEX loss, as measured by GC-FID, was extensive in the sediments which had a long history of pollution, with all compounds being utilized within 21–91 days in the most active cultures, and was very slight or non-existent in the pristine sediments. Also, the pattern of loss was different under the various reducing conditions within each sediment and between sediments. For example benzene loss was only observed in sulfidogenic cultures from the NY/NJ Harbor sediments while toluene was degraded under all redox conditions. The loss of BTEX was correlated to the reduction of the various electron acceptors. In cultures amended with gasoline the degradation was much slower and incomplete. These results show that the fate of the different BTEX components in anoxic sediments is dependent on the prevailing redox conditions as well as on the characteristics and pollution history of the sediment.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 35
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 10 (1999), S. 43-50 
    ISSN: 1572-9729
    Keywords: biodegradation ; nitrogen ; nutrients ; phenanthrene ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phenanthrene mineralization rates were found to vary widely among four soils; differences in soil nutrient levels was one hypothesis to explain this variation. To test this hypothesis, phenanthrene mineralization rates were measured in these soils with, and without, added nitrogen and phosphorus. Mineralization rates either remained unchanged or were depressed by the addition of nitrogen and phosphorus. Phenanthrene degradation rates remained unchanged in the soil which had the highest indigenous levels of nitrogen and phosphorus and which showed the largest increase in phosphorus levels after nutrients were added. The soils in which degradation rates were depressed had lower initial phosphorus concentrations and showed much smaller or no measurable increase in phosphorus levels after nutrients were added to the soils. To understand the response of phenanthrene degradation rates to added nitrogen and phosphorus, it may be necessary to consider the bioavailability of added nutrients and nutrient induced changes in microbial metabolism and ecology.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 36
    ISSN: 1572-9729
    Keywords: biodegradation ; growth inhibition ; metabolites ; toluene ; xylenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A biomass adapted to degrade toluene and xylenes in mixture was grown in a batch reactor with substrates supplied by pulses. The inhibition of biomass growth in the course of substrate degradation was investigated. The maximal biomass concentration of 7 g l−1 was obtained using 150 μl of toluene and 15 μl of a mixture of xylenes in one litre of liquid medium, and the maximal biomass productivity and yield were 53 mg l−1 h−1 and 0.32 gDW g s −1 , respectively. Higher quantities of substrate added by pulses, that is 200 μl of toluene with 20 μl of xylenes and 300 μl of toluene with 30 μl of xylenes, caused an accumulation of metabolites. These higher quantities of substrates caused inhibition of microbial growth. Among the metabolites produced, 4-methyl catechol was found in large quantities in the culture medium and in the cells.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 37
    ISSN: 1572-9729
    Keywords: Atrazine ; biodegradation ; chlorophenolics ; 2,4-D ; PCP ; pristine soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soils were sampled from two agricultural fields, two relatively pristine forests, and one suburban forest in Ontario, Canada. The ability of these soils to mineralize 2,4-dichlorophenoxyacetate, 3-chlorobenzoate, 4-chlorophenol, 2,4-dichlorophenol, pentachlorophenol, and atrazine was determined using 14C-labeled substrates. Direct preexposure was necessary before atrazine mineralization could be detected; however, it was not necessary for degradation of any of the other chemicals. 2,4-dichlorophenoxyacetate and pentachlorophenol mineralization was much higher in the agricultural soils relative to the pristine forest soils, but 3-chlorobenzoate and 2,4-dichlorophenol mineralization rates showed the opposite trend. Mineralization of 4-chlorophenol was about equivalent in all soils. Suburban forests soils were indistinguishable from agricultural soils with respect to their degradation of 2,4-dichlorophenoxyacetate and chlorobenzoate. Additionally, they were better able than any of the soils to withstand the toxic effects of pentachlorophenol. Pentachlorophenol mineralization was highly variable in the pristine forest soils, ranging from about 6 to 50%. Abiotic factors such as pH, soil type, and organic and moisture content did not account for these significant site differences. The selective forces responsible for these differences, and the possible differences in microbial populations are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 38
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 10 (1999), S. 169-176 
    ISSN: 1572-9729
    Keywords: biodegradation ; biofiltration ; bioremediation ; extremophile ; VOC ; waste gas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A biofilter treating alkylbenzene vapors was characterized for its optimal running conditions and kinetic parame-ters. Kinetics of the continuous biofilter were compared to batch kinetic data obtained with biofilm samples as well as with defined microbial consortia and with pure culture isolates from the biofilter. Both bacteria and fungi were present in the bioreactor. Five strains were isolated. Two bacteria, Bacillus and Pseudomonas, were shown to be dominant, as well as a Trichosporon strain which could, however, hardly grow on alkylbenzenes in pure culture. The remaining two strains were most often overgrown by the other three organisms in liquid phase batch cultures μ max, KS, KI values and biodegradation rates were calculated and compared for the difterent mixed and pure cultures. Since filter bed acidification was observed during biofiltration studies reaching a pH of about 4, experiments were also undertaken to study the influence of pH on performance of the different cultures. Biodegradation and growth were possible in all cases, over the pH range 3.5–7.0 at appreciable rates, both with mixed cultures and with pure bacterial cultures. Under certain conditions, microbial activity was even observed in the presence of alkylbenzenes down to pH 2.5 with mixed cultures, which is quite unusual and explains the ability of the present biocatalyst to remove alkylbenzenes with high efficiency in biofilters under acidic conditions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 39
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 10 (1999), S. 193-200 
    ISSN: 1572-9729
    Keywords: biodegradation ; butyl acetate ; selection of microorganisms ; silicon oil ; two-phase aqueous-organic system ; xylene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A stable microbial population, consisting of seven bacterial strains and three yeast strains, was selected in batch cultures on a mixture of ortho and meta-xylene and butyl acetate as the sole source of carbon and energy. This population can completely degrade up to 10 g/L of a mixture of these xenobiotics (70% xylene and 30% butyl acetate wt/wt) in a two-phase aqueous-silicone oil system (70%/30% vol/vol) within 96 h, while for the usual one-phase system very low growth degradation rates were observed. Further organic solvents were tested and finally, silicon oil was selected as the best organic phase for such a two-phase system. With periodical pH adjustments to 6.0 in fed-batch mode, the culture showed a global degradation rate of 63 mg L-1 h-1.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 40
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 10 (1999), S. 177-191 
    ISSN: 1572-9729
    Keywords: AQUASIM ; biodegradation ; biofilm ; growth ; kinetics ; methane ; modelling ; nitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This article discusses the growth of methanotrophic biofilms. Several independent biofilm growths scenarios involving different inocula were examined. Biofilm growth, substrate removal and product formation were monitored throughout the experiments. Based on the oxygen consumption it was concluded that heterotrophs and nitrifiers co-existed with methanotrophs in the biofilm. Heterotrophic biomass grew on soluble polymers formed by the hydrolysis of dead biomass entrapped in the biofilm. Nitrifier populations developed because of the presence of ammonia in the mineral medium. Based on these experimental results, the computer program AQUASIM was used to develop a biological model involving methanotrophs, heterotrophs and nitrifiers. The modelling of six independent growth experiments showed that stoichiometric and kinetic parameters were within the same order of magnitude. Parameter estimation yielded an average maximum growth rate for methanotrophs, μm, of 1.5 ± 0.5 d−1, at 20 °C, a decay rate, bm, of 0.24 ± 0.1 d−1, a half saturation constant, $${\text{K}}_{{\text{S(CH}}_{\text{4}} {\text{)}}} $$ , of 0.06 ± 0.05 mg CH4/L, and a yield coefficient, $$Y_{CH_4 } $$ , of 0.57 ±: 0.04 g X/g CH4. In addition, a sensitivity analysis was performed on this model. It indicated that the most influential parameters were those related to the biofilm (i.e. density; solid-volume fraction; thickness). This suggests that in order to improve the model, further research regarding the biofilm structure and composition is needed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 41
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 10 (1999), S. 219-233 
    ISSN: 1572-9729
    Keywords: bioavailability ; biodegradation ; bioreactor ; biotreatment ; NAPL ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two-liquid-phase culture systems involve the addition of a water-immiscible, biocompatible and non-biodegradable solvent to enhance a biocatalytic process. Two-liquid-phase bioreactors have been used since the mid-seventies for the microbial and enzymatic bioconversion of hydrophobic/toxic substrates into products of commercial interest. The increasing popularity of bioremediation technologies suggests a new area of application for this type of bioreactor. The toxicity and the limited bioavailability of many pollutants are important obstacles that must first be overcome in order to improve biodegradation processes. Two-liquid-phase bioreactors have the potential to resolve both limitations of biotreatment technologies by the enhancement of the mass-transfer rate of compounds with low bioavailability, and by the controlled delivery of apolar toxic compounds. This technology can also be useful in accelerating the enrichment of microorganisms degrading problematic pollutants. In this paper, we discuss the application of two-liquid-phase bioreactors to enhance the biodegradation of toxic/poorly bioavailable contaminants. Important microbial mechanisms involved in this type of system are described. Uptake of the substrates can be achieved by microorganisms freely dispersed in the aqueous phase and/or bound at the interface between the aqueous and the immiscible phases. Production of surface-active compounds and adhesion abilities are microbial features involved in the process. General guidelines for the design of two-liquid-phase bioreactors for biodegradation purposes are presented. Solvent selection should be established on specific criteria, which depend on the characteristics of target compound(s) and the microorganism(s) implicated in the biodegradation process. The central importance of maximizing the interfacial surface area is highlighted. The potential of this approach as an alternative to current biotreatment technologies is also discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 42
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 10 (1999), S. 353-362 
    ISSN: 1572-9729
    Keywords: mixed microbial community ; mono-chlorophenol ; biodegradation ; 3-chlorocatechol ; 5-chloro-2-hydroxymuconic semialdehyde ; meta- cleavage pathway
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A mixed microbial community, specially designed todegrade a wide range of substituted aromaticcompounds, was examined for its ability to degrademono-chlorophenols as sole carbon source in aerobicbatch cultures. The mixed culture degraded 2-, 3-, and4 -chlorophenol (1.56 mM) via a meta- cleavagepathway. During the degradation of 2- and3-chlorophenol by the mixed culture, 3-chlorocatecholproduction was observed. Further metabolism was toxicto cells as it led to inactivation of the catechol2,3-dioxygenase enzyme upon meta- cleavage of3-chlorocatechol resulting in incomplete degradation.Inactivation of the meta- cleavage enzyme led toan accumulation of brown coloured polymers, whichinterfered with the measurement of cell growth usingoptical denstiy. Degradation of 4-chlorophenol by themixed culture led to an accumulation of5-chloro-2-hydroxymuconic semialdehyde, themeta- cleavage product of 4-chlorocatechol. Theaccumulation of this compound did not interfere withthe measurement of cell growth using optical density.5-chloro-2-hydroxymuconic semialdehyde was furthermetabolized by the mixed culture with a stoichiometricrelease of chloride, indicating complete degradationof 4-chlorophenol by the mixed culture via ameta- cleavage pathway.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 43
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 109 (1999), S. 343-355 
    ISSN: 1573-2932
    Keywords: biodegradation ; crude oil ; salt marshes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Salt marsh ecosystems in Louisiana are at high risk of an oil contamination event while remediation of these systems is mainly limited to intrinsic bioremediation due to the physical sensitivity of salt marshes. This study investigated both the intrinsic and nutrient enhanced rates of crude oil degradation both in microcosm and core studies. In addition, limiting elements, loading rates and optimum nitrogen forms (NH 4 + or NO 3 - ) were determined. Salt marshes have relatively low intrinsic degradation rates (0–3.9% day-1) of the alkane component (C11-C44) but high rates (8–16% day-1) of degradation of the polycyclic aromatic hydrocarbon (PAH) fraction (naphthalene, C1, and C2-Naphthalene and Phenanthrene, C1, and C2-Phenanthrene). Additions of nitrogen statistically enhanced degradation of many alkanes and total PAHs while naturally present phosphorous was found to be sufficient. Nitrogen was found to be most effective if applied as NH 4 + in the range of 100-500-N mg kg-1 of soil producing a pore water range of 100-670-N mg L-1. Core studies indicate that similar trends are observed when applying fertilizers to intact portions of salt marsh.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 44
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 110 (1999), S. 1-16 
    ISSN: 1573-2932
    Keywords: contamination ; heay metal ; immobilization ; lead ; remediation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Lead contamination is of great environmental concern due to its effect on human health. This study investigated the feasibility of using Florida phosphate rocks (PR) to immobilize aqueous Pb from Pb-contaminated soils. Occidental Chemical PR effectively immobilized Pb from 13 Pb-contaminated soils, but its effectiveness was affected by soil pH and extent of Pb contamination, with aqueous Pb reduction ranging from 21.8 to 100%. Longer reaction times improved aqueous Pb reduction. Six out of 7 Florida PR effectively immobilized aqueous Pb from the Burch Loam soil. Phosphate rock from Cargil Fertilizer was ineffective, with aqueous Pb reduction of 4.73%. The relative effectiveness of 6 Florida PR to immobilize aqueous Pb, excluding Cargil Fertilizer PR, was: Occidental Chemical 〉 CF Chemical 〉 Nu Gulf Industries 〉 Agri-Chemical 〉 IMC 〉 Agrico Chemical. Phosphate rock solubility (as estimated by acid extraction and X-ray diffraction) failed to predicate the effectiveness of PR to immobilize aqueous Pb from Pb-contaminated soils, partially due to the complexity and kinetics of reactions between PR and soils. Our results demonstrate that PR has a potential to cost-effectively treat Pb-contaminated soils.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 45
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 115 (1999), S. 465-479 
    ISSN: 1573-2932
    Keywords: gravel sludge ; heavy metals ; immobilization ; soil remediation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Field plot studies were conducted on sandy loam soils in Dottikon, Rafz and Giornico contaminated by Zn, Cu and Cd to investigate the effectiveness of gravel sludge as an in situ immobilization additive. In addition to NaNO3-extractable heavy metal concentrations in the soil, metal concentrations in ryegrass (Lolium perenne L.) and lettuce (Lactuca sativa L.) were analyzed at two application doses of gravel sludge. Application of gravel sludge increased pH in all three topsoils by up to 0.6 units and reduced NaNO3-extractable Zn concentrations by more than 65%. No effect was seen for the NaNO3-extractable Cu concentrations in Rafz and even an increase resulted in Giornico. In Dottikon, gravel sludge additions decreased the NaNO3-extractable Cu concentrations from 0.97 to 0.7 mg kg−1 and reduced the concentrations of Zn and Cu by more than 35% in ryegrass. For lettuce grown in Giornico and Dottikon, we found reductions of Zn and Cd concentrations by 22 to 48%, whereas in Rafz, no significant variations were found. Immobilization by gravel sludge was most efficient in soils with high total/NaNO3-extractable heavy metal ratio and more effective for ryegrass than for lettuce. Part of the immobilization effect was due to a pH increase, caused by CaCO3 in gravel sludge.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 46
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 111 (1999), S. 1-18 
    ISSN: 1573-2932
    Keywords: biodegradation ; hydrocarbon ; nitrogen ; nutrient ratios ; phosphorus ; soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The biodegradation of hexadecane (C as hexadecane-C) was assessed under 34 different external nitrogen (N supplied as NO3-N) and phosphorus (P supplied as PO4-3-P) supply conditions in order to determine how different nutrient formulations affected nutrient limitation conditions during degradation. CO2 production yields indicated that shifts in N and P supply levels resulted in variable biodegradation responses due to shifts in the limiting-nutrient (e.g., from N to P). For example, the estimated maximum fractional CO2 yield ratio was 0.24 (mg CO2-C produced mg-1 hexadecane-C) for P-limited nutrient formulations (P:hexadecane-C〈0.01), whereas the yield ratio was more than two times greater when the system was not P- limited. Similar effects were observed for N-limited (N:hexadecane- C〈0.15) versus non-N-limited formulations. The relative bioavailability of natural soil-N and soil-P also was examined. In the soil studied, background soil-N was 96.3% organic-N and was found to be largely nonbioavailable. In contrast, high CO2 yields were observed even when no external P was supplied. An iterative mathematical procedure indicated that the Olsen soil-P subfraction (inorganic soil-P plus soluble organic soil- P) best approximated bioavailable soil-P for this soil. Our results indicate that both N and P additions affect biodegradation yields, but that stoichiometrically inappropriate nutrient mixes produce suboptimal CO2 yields. We also found that the bioavailable fractions of soil-N and soil-P should be incorporated into estimating the most suitable nutrient formulations for a given contamination scenario.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 47
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 515-516 
    ISSN: 1573-0972
    Keywords: Biofilter ; biodegradation ; effluent ; fertilizer ; immobilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A biofilter composed of yeasts and cassava peel was used to detoxify fertilizer plant effluent. The biological oxygen demand was reduced on treatment from a range of 1200–1400 mg/l to a range 135–404 mg/l. The ammonia-nitrogen (NH3–N) and nitrate-nitrogen (NO3–N) were reduced after treatment from 1000 to 10 mg/l and from 100 to 17.6 mg/l, respectively. The biofilter is simple and easy to handle with high efficiency of 98%.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 48
    ISSN: 1573-0972
    Keywords: Adhesion ; cotton threads ; immobilization ; invert sugar ; microbial filter ; polyethylenimine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Polyethylenimine(PEI)-coated cotton threads were shown to have potential for reducing microbial load from a flowing suspension. Turbid cell suspensions perfused through the PEI column appeared as totally clear in the effluent. The adhesion efficiency of the matrix was found to depend on the concentration of PEI used to treat the threads. Threads coated with 2.5% PEI were found to show optimal retention of cells. A considerable amount of binding was seen over a broad range of ionic concentration (0–0.3 M) and pH (3.6–10.3). Under similar conditions control threads did not show any filtration capacity. Saccharomyces cerevisiae, Saccharomyces fragilis, Escherichia coli and an Acetobacter species could be effectively filtered using PEI-coated threads. This technique can find potential for the simultaneous filtration and immobilization of cells in a bioreactor to be used in continuous bioprocessing as exemplified for the inversion of sucrose syrups using baker's yeast. The bioreactor could continuously hydrolyse 60% (w/v) sucrose syrups with a productivity of 2.25 kg/day for over a month without loss in efficiency.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 49
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 6 (1998), S. 115-120 
    ISSN: 1572-8900
    Keywords: Polyaromatics ; free-radical polymerization ; biodegradation ; peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Polymers formed from peroxidase-based free-radical polymerization reactions were characterized for rates of mineralization against lignin and humic acid controls. Degradation studies were carried out in soil systems over 202 days and cumulative net CO2 was determined. Whereas mineralization of the humic acid and alkali lignin controls totaled ca. 20% at the end of the test exposure, there was essentially no net mineralization of the hydrolytic lignin control. Mineralization of the test samples totaled 5% for poly(p-ethylphenol) and 11% for poly(m-cresol). At the same time, mineralization of the poly(p-phenyl phenol) totaled 64%. Conversely, the readily biodegradable polymers cellulose and PHB reached values of 91 to 97% in less than 60 days. Our data suggest that the mineralization kinetics of the enzymatically derived polyaromatics mimic those of the naturally occurring heteropolymers.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 50
    ISSN: 1572-8900
    Keywords: Nonionic surfactants ; biodegradation ; alkylphenol poly(ethoxylate)s ; alkyl poly(ethoxylate)s ; polyethylene glycol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Different bacterial strains able to attack polyoxyethylene-type nonionic surfactants were isolated by enrichment procedure from the surface waters of the Arno River. Alkylphenol polyethoxylates and alkyl polyethoxylates, as well as polyethylene glycols, were degraded and assimilated by bacterial strains in axenic cultures. Degradative routes of polyethyleneoxide chains were investigated by matching each bacterial isolate with several types of nonionic surfactants and polyethers and by the identification of their degradation products isolated during aerobic digestion experiments. In accordance with previous reports, the first attack led to the shortening of the poly(oxyethylene) chains of the nonionic surfactants. It was found that the strains able to degrade PEG segments of nonionic surfactants possess enzymatic systems unable to degrade free PEGs, whereas those degrading the latter substrates cannot degrade PEG segments coupled to hydrophobic moieties.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 51
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 6 (1998), S. 23-29 
    ISSN: 1572-8900
    Keywords: Polyethylene ; polystyrene ; biodegradation ; copolymerization ; hydrolytic degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract 2-Methylene-1,3-dioxepane (MDP) was copolymerized with ethylene (E) at a pressure of approximately 1000 psi and a temperature of approximately 70°C with AIBN as the free radical initiator. The copolymers obtained, poly(MDP-co-E), were characterized by elemental analysis, IR, 1H-NMR and 13C-NMR spectroscopy, DSC, and GPC. The copolymers contained 2–15 mol% ester units. MDP was also copolymerized with styrene (S) at 120°C with di-t-butyl peroxide as the initiator to prepare the copolymer, poly(MDP-co-S). The number-average molecular weights of both types of copolymers were in the range of 6000 to 11,000, and the weight-average molecular weights were in the range of 9000 to 17,000. The melting temperatures of poly(MDP-co-E) decreased with increasing ester unit content in the copolymer. For the MDP-S copolymers, the glass transition temperatures decreased with increasing ester unit content. Both poly(MDP-co-E) and poly(MDP-co-S) were degraded by methanolysis, and their molecular weights decreased by the expected amounts based on the ester unit content.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 52
    ISSN: 1572-8900
    Keywords: Starch ; PHBV ; PHA ; plastic ; blends ; biodegradation ; soil ; compost
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Injection molded specimens were prepared by blending poly (hydroxybutyrate-co-valerate) (PHBV) with cornstarch. Blended formulations incorporated 30% or 50% starch in the presence or absence of poly-(ethylene oxide) (PEO), which enhances the adherence of starch granules to PHBV. These formulations were evaluated for their biodegradability in natural compost by measuring changes in physical and chemical properties over a period of 125 days. The degradation of plastic material, as evidenced by weight loss and deterioration in tensile properties, correlated with the amount of starch present in the blends (neat PHBV 〈 30% starch 〈 50% starch). Incorporation of PEO into starch-PHBV blends had little or no effect on the rate of weight loss. Starch in blends degraded faster than PHBV and it accelerated PHBV degradation. Also, PHBV did not retard starch degradation. After 125 days of exposure to compost, neat PHBV lost 7% of its weight (0.056% weight loss/day), while the PHBV component of a 50% starch blend lost 41% of its weight (0.328% weight loss/day). PHB and PHV moieties within the copolymer degraded at similar rates, regardless of the presence of starch, as determined by 1H-NMR spectroscopy. GPC analyses revealed that, while the number average molecular weight (Mn) of PHBV in all exposed samples decreased, there was no significant difference in this decrease between neat PHBV as opposed to PHBV blended with starch. SEM showed homogeneously distributed starch granules embedded in a PHBV matrix, typical of a filler material. Starch granules were rapidly depleted during exposure to compost, increasing the surface area of the PHBV matrix.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 53
    Electronic Resource
    Electronic Resource
    Springer
    Journal of polymers and the environment 6 (1998), S. 197-202 
    ISSN: 1572-8900
    Keywords: Starch ; cellulose ; biodegradation ; ISO/DIS 14855:1997 ; ASTM D 5338-92 ; priming effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In order to verify the response of the controlled composting test method (i.e., the ISO/DIS 14855:1997, the ASTM D 5338-92, or the CEN counterpart) to starch at different concentrations, the maximum amount prescribed by the test method (100 g) and lower amounts (60 and 30 g), as if starch were a coingredient in a blend, were tested. After 44 days of incubation (at a constant temperature of 58°C) the biodegradation curves were in a plateau phase, displaying the following final values (referred to a nominal starch initial amount of 100 g): starch 100 g, 97.5%; starch 60 g, 63.7%; and starch 30 g, 32.5%. The data show a CO2 evolution roughly equal, in each case, to the theoretical maximum, indicating a complete starch mineralization. We cannot discern whether the deviations found at lower concentrations are caused by a priming effect. In any case, the extent of the deviations is not high and is acceptable in biodegradation studies. The average biodegradation of cellulose, obtained gathering four independent experiments with 11 biodegradation curves, turned out to be 96.8 ± 6.7% (SD) after 47 ± 1 days. The data indicate that the controlled composting is a reliable test method also for starch and cellulose and, consequently, for starch-based and cellulose-based materials.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 54
    ISSN: 1572-8900
    Keywords: LDPE ; biodegradation ; molecular weight changes ; degradation products
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The molecular weight changes in abiotically and biotically degraded LDPE and LDPE modified with starch and/or prooxidant were compared with the formation of degradation products. The samples were thermooxidized for 6 days at 100°C to initiate degradation and then either inoculated with Arthobacter paraffineus or kept sterile. After 3.5 years homologous series of mono- and dicarboxylic acids and ketoacids were identified by GC-MS in abiotic samples, while complete disappearance of these acids was observed in biotic environments. The molecular weights of the biotically aged samples were slightly higher than the molecular weights of the corresponding abiotically aged samples, which is exemplified by the increase in $$\overline M _n$$ from 5200 g/mol for a sterile sample with the highest amount of prooxidant to 6000 g/mol for the corresponding biodegraded sample. The higher molecular weight in the biotic environment is explained by the assimilation of carboxylic acids and low molecular weight polyethylene chains by microorganisms. Assimilation of the low molecular weight products is further confirmed by the absence of carboxylic acids in the biotic samples. Fewer carbonyls and more double bonds were seen by FTIR in the biodegraded samples, which is in agreement with the biodegradation mechanism of polyethylene.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 55
    ISSN: 1572-8900
    Keywords: Poly(ε-caprolactone ; poly(lactic acid) ; processing additives ; biodegradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Melt-pressed films of polycaprolactone (PCL) and poly(lactic acid) (PLA) with processing additives, CaCO3, SiO2, and erucamide, were subjected to pure fungal cultures Aspergillus fumigatus and Penicillium simplicissimum and to composting. The PCL films showed a rapid weight loss with a minor reduction in the molecular weight after 45 days in A. fumigatus. The addition of SiO2 to PCL increased the rate of (bio)erosion in A. fumigatus and in compost. The use of a slip additive, erucamide, was shown to modify the properties of the film surface without decreasing the rate of bio(erosion). Both the rate of weight loss and the rate of molecular weight reduction of PCL increased with decreasing film thickness. The addition of CaCO3 to PLA significantly reduced the thermal degradation during processing, but it also reduced the rate of the subsequent (bio)degradation in the pure fungal cultures. PLA without additives and PLA containing SiO2 exhibited the fastest (bio)degradation, followed by PLA with CaCO3. The degradation of the PLA films was initially governed by chemical hydrolysis, followed by an acceleration of the weight change and of the molecular weight reduction. PLA film subjected to composting exhibits a rapid decrease in molecular weight, which then remains unchanged during the measurement period, probably because of crystallization.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 56
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 9 (1998), S. 285-292 
    ISSN: 1572-9729
    Keywords: biodegradation ; gypsum ; hydrogen ; inhibition ; isolation ; sulfate-reducing bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Eleven pure strains of sulfate-reducing bacteria have been isolated from lab-scale bioreactors or gypsum disposal sites, all featuring relatively high concentrations of sulfate, and from natural environments in order to produce sulfide from gypsum using hydrogen as energy source. The properties of the eleven strains have been investigated and compared to these of three collection strains i.e. Desulfovibrio desulfuricans and Dv. vulgaris and Desulfotomaculum orientis. Particular attention was paid to the volumetric and specific sulfide production rate and to the hydrogen sulfide inhibition level. By comparison to the three collection strains, a 75% higher production rate and a hydrogen sulfide inhibition level about twice as high i.e. 25.1 mM have been achieved with strains isolated from sulfate-rich environments. The strain selection, particularly from sulfate-rich environments, should be considered as an optimization factor for the sulfate reduction processes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 57
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 9 (1998), S. 343-357 
    ISSN: 1572-9729
    Keywords: biodegradation ; condensed tannins ; gallic acid ; hydrolysable tannins ; quercetin ; rumen ; tannase ; tannins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tannins are water-soluble polyphenolic compounds having wide prevalence in plants. Hydrolysable and condensed tannins are the two major classes of tannins. These compounds have a range of effects on various organisms – from toxic effects on animals to growth inhibition of microorganisms. Some microbes are, however, resistant to tannins, and have developed various mechanisms and pathways for tannin degradation in their natural milieu. The microbial degradation of condensed tannins is, however, less than hydrolysable tannins in both aerobic and anaerobic environments. A number of microbes have also been isolated from the gastrointestinal tract of animals, which have the ability to break tannin-protein complexes and degrade tannins, especially hydrolysable tannins. Tannase, a key enzyme in the degradation of hydrolysable tannins, is present in a diverse group of microorganisms, including rumen bacteria. This enzyme is being increasingly used in a number of processes. Presently, there is a need for increased understanding of the biodegradation of condensed tannins, particularly in ruminants.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 58
    ISSN: 1572-9729
    Keywords: acrylamide ; amidase ; biodegradation ; biotransformation ; immobilized cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influences of concentration of acrylamide, pH, temperature, duration of storage of encapsulated cells and presence of different metals and chelators on the ability of immobilized cells of a Rhodococcus sp. to degrade acrylamide were evaluated. Immobilized cells (3 g) rapidly degraded 64 and 128 mM acrylamide in 3 and 5 h, espectively, whereas free cells took more than 24 h to degrade 64 mM acrylamide. An acrylamide concentration of 128 mM inhibited the growth of the free cells. Immobilized bacteria were slow to degrade acrylamide at 10 °C. Less than 60% of acrylamide was degraded in 4 h. However, 100% of the compound was degraded in less than 3 h at 28 °C and 45 °C. The optimum pH for the degradation of acrylamide by encapsulated cells was pH 7.0. Less than 10% of acrylamide was degraded at pH 6.0, while ca. 60% of acrylamide was degraded at pH 8.0 and 8.5. Copper and nickel inhibited the degradation, suggesting the presence of sulfhydryl (-SH) groups in the active sites of the acrylamide degrading amidase. Iron enhanced the rates of degradation and chelators (EDTA and 1,10 phenanthroline) reduced the rates of degradation suggesting the involvement of iron in its active site(s) of the acrylamide-degrading-amidase. Immobilized cells could be stored up to 10 days without any detectable loss of acrylamide-degrading activity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 59
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 9 (1998), S. 393-409 
    ISSN: 1572-9729
    Keywords: biodegradation ; bioreactors ; NMR imaging ; NMR spectroscopy ; nutrient removal ; sludge ; solid waste
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper gives an introduction to nuclear magnetic resonance spectroscopy (NMR) and magnetic resonance imaging (MRI) in relation to applications in the field of environmental science and engineering. The underlying principles of high resolution solution and solid state NMR, relaxation time measurements and imaging are presented. Then, the use of NMR is illustrated and reviewed in studies of biodegradation and biotransformation of soluble and solid organic matter, removal of nutrients and xenobiotics, fate of heavy metal ions, and transport processes in bioreactor systems.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 60
    ISSN: 1572-9729
    Keywords: biodegradation ; biodeterioration ; coatings ; electrochemical impedance spectroscopy ; fungi ; polyimides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reports results of biodegradation studies of polyimide coatings exposed to a mixed fungal culture using electrochemical impedance spectroscopy (EIS). The fungal consortium was originally isolated from degraded polyimides and identified species include Aspergillus versicolor, Cladosporium cladosporioides, and a Chaetomium species. Actively growing fungi on polyimides yield distinctive EIS spectra through time, indicative of failure of the polymer integrity compared to the uninoculated controls. An initial decline in coating resistance was related to the partial ingress of water molecules and ionic species into the polymeric matrices. This was followed by further degradation of the polymers by activity of the fungi. The relationship between the changes in impedance spectra and microbial degradation of the coatings was further supported by scanning electron microscopy, showing extensive colonization of the polyimide surfaces by the fungi. Our data indicate that EIS can be a sensitive and informative technique for evaluating the biosusceptibility of polymers and coatings.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 61
    ISSN: 1572-9729
    Keywords: alkanes ; biodegradation ; Candida tropicalis ; crude oil ; gas chromatography ; petroleum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five microorganisms, three bacteria and two yeasts, capable of degrading Tapis light crude oil were isolated from oil-contaminated soil in Bangkok, Thailand. Soil enrichment culture was done by inoculating the soil in mineral salt medium with 0.5% v/v Tapis crude oil as the sole carbon source. Crude oil biodegradation was measured by gas chromatography method. Five strains of pure microorganisms with petroleum degrading ability were isolated: three were bacteria and the other two were yeasts. Candida tropicalis strains 7Y and 15Y were identified as efficient oil degraders. Strain 15Y was more efficient, it was able to reduce 87.3% of the total petroleum or 99.6% of n-alkanes within the 7-day incubation period at room temperature of 25 ± 2 °C.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 62
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 9 (1998), S. 91-102 
    ISSN: 1572-9729
    Keywords: activated sludge ; biodegradation ; biological degradation ; lyocell fibres ; N-methylmorpholine-N-oxide ; wastewater treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract N-Methylmorpholine-N-oxide (NMMO) is capable of dissolving cellulose without any further addition of chemicals. The solution can be used to produce cellulosic staple fibres by pressing it through spinning jets into an aqueous spinning bath. Because of results from conventional biodegradation tests using non-adapted activated sludge, the solvent is generally considered being persistent. The object of the described work was to show, whether and how activated sludge can be adapted to N-methylmorpholine-N-oxide and whether it is possible to purify NMMO-containing wastewaters in conventional wastewater treatment plants. The experiments showed that the sludge can be adapted within about 15–20 days. Adapted sludge can degrade the substance itself and its most important metabolites to concentrations below their detection levels and retain this ability even during limited periods without solvent being present in the wastewater. The main requirement for a successful adaptation is a high sludge age. The degradation takes place in several steps. First, NMMO is reduced to N-methylmorpholine. The next step is a demethylation of N-methylmorpholine to morpholine. This step is crucial for the adaptation process. Once morpholine has been formed, the adaptation proceeds very quickly until none of the substances in question can be detected any longer. So the next step must be the cleavage of the morpholine ring structure.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 63
    ISSN: 1572-9729
    Keywords: biodegradation ; fluorophenols ; 19 F NMR ; oxidative defluorination ; Rhodococcus species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Of all NMR observable isotopes 19F is the one perhaps most convenient for studies on biodegradation of environmental pollutants. The reasons underlying this potential of 19F NMR are discussed and illustrated on the basis of a study on the biodegradation of fluorophenols by four Rhodococcus strains. The results indicate marked differences between the biodegradation pathways of fluorophenols among the various Rhodococcus species. This holds not only for the level and nature of the fluorinated biodegradation pathway intermediates that accumulate, but also for the regioselectivity of the initial hydroxylation step. Several of the Rhodococcus species contain a phenol hydroxylase that catalyses the oxidative defluorination of ortho-fluorinated di- and trifluorophenols. Furthermore, it is illustrated how the 19F NMR technique can be used as a tool in the process of identification of an accumulated unknown metabolite, in this case most likely 5-fluoromaleylacetate. Altogether, the 19F NMR technique proved valid to obtain detailed information on the microbial biodegradation pathways of fluorinated organics, but also to provide information on the specificity of enzymes generally considered unstable and, for this reason, not much studied so far.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 64
    ISSN: 1573-0778
    Keywords: concanavalin A ; cytotoxic T lymphocytes ; immobilization ; interleukin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Human tumor-specific CD4+ cytotoxic T lymphocytes (CTL) were generated against duodenum papilloma cell line TGBC18TKB from HLA type-matched peripheral blood mononuclear cells. Concanavalin A (Con A) immobilized on carrier beads stimulated growth of the CTL in a long-term culture without repeated antigen stimulation, while soluble Con A induced death of the CTL. The CTL exhibited the target-specific cytotoxicity in a more potent manner than those before the long-term culture in the presence of the immobilized Con A. Enhanced expression of the adhesion molecule, CD11b, was observed on the CTL. These results suggest that immobilized Con A will be useful for continuous growth stimulation and large scale expansion of CTL without tumor antigen.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 65
    ISSN: 1573-2932
    Keywords: artificial wetlands ; biodegradation ; hydrocarbon ; Lemna minor ; Typha latifolia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract An artificial wetland planted with Typha latifolia was fed during a 360-day experiment with a reconstituted hydrocarbon wastewater (60 ppm, 850 L day−1). Concentrations and chemical composition were periodically monitored. The epuration efficiency was studied together with the accumulation in sediment and the bacterial development. The apparent effluent concentration was below 8 mg L−1 and the decrease in hydrocarbon concentration raised 90%. pH ranged between 6.9 and 8 and Total Suspended Solids (TSS) were below 10 mg L−1. Hydrocarbon amount accumulated in sediment was estimated to be less than 10% of the input amount. We observed a high development of aerobic heterotrophic bacteria (106 bac mL−1) and hydrocarbon-utilizing bacteria (105 bac mL−1), which probably interacted with the plants for the biodegradation of hydrocarbon (in the saturated effluent fraction, normal alkane biodegradation amounted to approximatively 80%). A comparative system with floating plants (Lemma minor), named ‘control bed’, was studied in parellel and showed lower performances.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 66
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 104 (1998), S. 285-304 
    ISSN: 1573-2932
    Keywords: biodegradation ; ethanol ; extraction ; PAHs ; polyaromatic hydrocarbons ; solvents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The biodegradation of polyaromatic hydrocarbons (PAHs) has been well documented; however, the biodegradation of PAHs in contaminated soil has proved to be problematic. Sorption of PAHs to soil over time can significantly decrease their availability for extraction much less than for biodegradation. In this study the ability of various organic solvents to extract PAHs from coal tar-contaminated soil obtained from former manufactured gas plant (MGP) sites was investigated. Solvents investigated included acetone/hexane, dichloromethane, ethanol, methanol, toluene, and water. The extraction of MGP soils with solvents was investigated using soxhlet extraction, multiple soxhlet extractions, sonication, and brief agitation at ambient temperature with a range of solvent concentrations. Of particular interest was the documentation of the recalcitrance of PAHs in weathered MGP soils to extraction and to bioremediation, as well as to demonstrate the ease with which PAHs extracted from these soils can be biodegraded. The efficiency of extraction of PAHs from MGP soils was found to be more dependent upon the choice of solvent. The environmentally-benign solvent ethanol, was shown to be equal to if not better than acetone/hexane (the EPA recommended solvent) for the extraction of PAHs from MGP soils, brief contact/agitation times (minutes) using small quantities of ethanol (2 volumes or less) can achieve nearly quantitative extraction of PAHs from MGP soils. Moreover aqueous slurries of an MGP soils experienced less than 10% biodegradation of PAHs in 14 days while in the same period about 95% biodegradation was acieved using PAHs extracted from this soil by ethanol and subsequently added to aqueous bacterial suspensions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 67
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 106 (1998), S. 111-122 
    ISSN: 1573-2932
    Keywords: silicone ; silanol ; environmental ; biodegradation ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Dimethylsilanediol (DMSD) is the ultimate hydrolysis product of silicone (polydimethylsiloxane = PDMS) polymer in soil. Our previous paper showed that it would volatilize from soil, and the present study investigates the importance of microbial degradation in removing DMSD from soil. DMSD (14C-labeled) was thus incubated (1 mg kg-1) for 30 wk at 25 °C in soils from a permanent grass field, a corn field, a deciduous woodland, and a pine woodland. Release of14 CO2 varied from 0.4 to 1.6% wk-1. For 3 of the soils, 14CO2 increased with higher microbial biomass, while organisms in the deciduous woodland soil were more active in degrading DMSD than organisms in the other soils. After 30 weeks, most of the remaining 14C in the soil had moved from freely available water extractable to less available acid and base extractable fractions. Similar incubations with 2% plant litter showed extensive transfer of the DMSD into the litter layer. Incubations with a microbial inhibitor showed less DMSD degradation, while cold storage of soils almost completely stopped degradation. These results suggest that microbial degradation is an important mechanism of DMSD loss from soil.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 68
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 108 (1998), S. 129-148 
    ISSN: 1573-2932
    Keywords: biodegradation ; leaching ; soil ; triazine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The purpose of this study was to investigate the fate of triazines and their metabolites in the unsaturated zone in the field conditions during a period from the spring sowings to the autumn tillages. The study was performed upon two soils treated for corn culture during the year 1992. The investigations were presented after the wet weather by subsurface drainage and surface runoff. Throughout the 6 months survey, the relative importance of the triazines is by decreasing order: atrazine 〉 simazine 〉 desethylatrazine (DEA). After the application, the herbicides were only found in the first 40 cm of the soil during the dry period of the cultivation. They were dragged towards the depth by percolation water mainly during the first water infiltrations when the drains were set in again in autumn or winter. The losses of herbicides after the cultivation cycle (october) expressed as a ratio of the initial input ranged from 40 to 73% for the atrazine and from 40 to 44% for the simazine and depended on the importance of the application amount. In autumn and winter, the losses originating in percolation were higher than those due to volatilization and/or degradation (abiotic and biodegradation), as the soil conditions were unpropicious to the formation of non-extractable residues.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 69
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 447-450 
    ISSN: 1573-0972
    Keywords: Claviceps ; ergot alkaloids ; immobilization ; surfactant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In a semicontinuous process immobilized Claviceps paspali mycelia produced alkaloids over a period of 60 days (six reincubations). By addition of the surfactant Pluronik, a polyethoxypolypropoxy polymer, a considerable increase in alkaloid biosynthesis occurred. The maximum product concentration achieved was 8.35gl-1, and the overall productivity was 5.80 mgl-1 h-1, which is half the productivity of the batch process. Maximum process productivity for a single reincubation (12.3 mg l-1 h-1) was almost equal to the batch process productivity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 70
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 927-929 
    ISSN: 1573-0972
    Keywords: Urease ; pigeonpea ; Cajanus cajan ; immobilization ; urea analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Urease of pigeonpea has been immobilized on polyethyleneimine-activated cotton cloth followed by cross-linking with dimethyl suberimidate. Optimum immobilization (56%) was obtained at a protein loading of 1.2mg/5×5cm2 cloth piece. The immobilized enzyme stored in 0.1M Tris/acetate buffer, pH6.5, at 4°C had a t1/2 of 70 days. There was practically no leaching of the enzyme from the immobilization matrix in 15 days. The immobilized enzyme was used 7 times at an interval of 24h between each use with 75% residual activity at the end of the period. Blood urea analysis was carried out with immobilized urease for some clinical samples.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 71
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 343-348 
    ISSN: 1573-0972
    Keywords: Biofilter ; immobilization ; malodour ; volatile fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract n-Valeric acid, one of the main malodorous pollutants from livestock houses was eliminated with a biofilter prepared with Rhodococcus sp. B261 immobilized onto ceramic beads. The strain was isolated from composted pig faeces and grown in an artificial medium containing volatile fatty acids as a carbon source. The cells were immobilized onto ceramic beads in vacuo. The beads were aseptically incubated at 37 °C, pH 8.0, for 24h for activation of the cells. The beads with immobilized cells (3.36×109 c.f.u./g ceramic beads) and moisture content of 35% (w/w) were packed into a glass column equipped with a water jacket to keep the temperature constant. One hundred-seventy ppm of gaseous n-valeric acid were removed for 11 days at 30h -1 (space velocity) and 37 °C.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 72
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 57 (1998), S. 145-154 
    ISSN: 0006-3592
    Keywords: bioavailability ; PAH ; biodegradation ; dissolution ; hydrodynamic ; mixing ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of hydrodynamic conditions on the dissolution rate of crystalline naphthalene as a model polycyclic aromatic hydrocarbon (PAH) was studied in stirred batch reactors with varying impeller speeds. Mass transfer from naphthalene melts of different surface areas to the aqueous phase was measured and results were modeled according to the film theory. Results were generalized using dimensionless numbers (Reynolds, Schmidt, and Sherwood). In combined mass transfer and biodegradation experiments, the effect of hydrodynamic conditions on the degradation rate of naphthalene by Pseudomonas 8909N was studied. Experimental results were mathematically described using mass-transfer and microbiological models. The experiments allowed determination of mass-transfer and microbiological parameters separately in a single run. The biomass formation rate under mass transfer limited conditions, which is related to the naphthalene biodegradation rate, was correlated to the dimensionless Reynolds number, indicating increased bioavailability at increased mixing in the reactor liquid. The methodology presented in which mass transfer processes are quantified under sterile conditions followed by a biodegradation experiment can also be adapted to more complex and realistic systems, such as particulate, suspended PAH solids or soils with intrapartically sorbed contaminants when the appropriate mass-transfer equations are incorporated. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 145-154, 1998.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 73
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 61-67 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Risk assessments have been performed to determine the risk associated with the transportation of hazardous wastes through a city. In the course of these assessments, a number of modeling issues arose relating to transportation accident rates, the characterization of incidents, the effect of thermal radiation, the impact of exposure to toxic chemicals, and the threshold for acceptable risk. This paper discusses these issues.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 74
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. S3 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 75
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 98-103 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper presents the design of ribbon wound pressure vessels useful for Ammonia, Urea and Methanol plants. The design is to create a thin shell of 1/5 the total wall thickness required, weld it to the end pieces, and wind 4 to 8 mm thick ribbons of 80 mm width at an angle of 15 to 30 degrees on the inner shell, using a prestress. The ribbons are welded at the ends and an even number of layers are wound cross-helically on to the shell. With more than 7000 vessels over the pressure range of 50 to 350 atmospheres in use in the various chemical industries in China over the past 30 years, their safety record has been excellent. Of particular interest has been the application of this technology in the Ammonia and Urea plants, where the design allows fabrication of these vessels at substantial reduction in cost, and early delivery, when compared to the mono wall technology.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 76
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 20-22 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Most audits try to look at a representative selection of the plant procedures and equipment. An alternative is a survey, a look in depth at selected procedures (such as those for testing alarms and trips, issuing permits-to-work, controlling modifications, taking samples or testing relief devices) or selected equipment (such as level glasses or equipment for handling LPG). If the procedure or equipment is well-chosen, surveys may make a bigger contribution to safety, per person-hour, than a conventional audit.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 77
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 39-42 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Under OSHA 1910.119, all Process Safety Management (PSM) facilities are required to keep their pressure relief system design information current. This article demonstrates why a pressure relief system design verification effort must be based on an equipment list, rather than a relief device list, in order to ensure that every piece of equipment is adequately protected. The formerly common practice of simply checking the design bases of all existing relief devices is deficient is deficient since this technique does not systematically ensure that every piece of equipment is protected.The “Berwanger Method” is a step by step process for designing or analyzing a pressure relief system to meet OSHA 1910.119 Process Safety Information (PSI) and Process Hazard Analysis (PHA) mandates. The method uses a relational database which tracks the relationships between protected equipment, potential overpressure scenarios, and protective devices.The challenge facing an operating company does not end once the design basis has been “verified” - the design basis information must also be maintained and be readily accessible to avoid costly reinvention of the wheel down the road. The “Berwanger Method” also addresses these maintenance issues.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 78
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 49-60 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper reports on a comprehensive literature search and small scale experimental work on the reaction characteristics of phosphorous trichloride and water. More than 30 tests were conducted, including both closed and open test cells. The water to phosphorus trichloride molar ratio was varied from 1 to 25. When in contact, water and phosphorus trichloride will form two liquid layers with a reaction starting at the interface. The impact of variables on reaction rates including the interface surface area, layer depth, and stirring were investigated experimentally. A reaction rate model that fits all the measured data is presented. Case studies illustrating the use of this data for emergency relief systems and vent containment design are presented in reference. [1].
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 79
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 68-73 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two major accidents in the 80's: the summit Tunnel Fire, England and Piper Alpha disaster, an offshore platform in the North Sea; and very recently, possible explosion of the Boeing, TWA flight 800 at New York, makes it imperative that further research into the mechonisms of the ignition of flammable vapor/air mixture in contact with hot surfaces needs to be done. There have been a number of studies of ignition by hot surfaces, but in all these studies the ignition sources were wire, sphere or strip, i.e., most of them were flat surfaces. But to the authors' knowledge, other variables which affect the ignition mechanism such as irregular geometrical shapes have not been studied. The purpose of this paper is to examine how the degree of confinement (or, configuration), size and orientation, of the heated surface affects the ignition temperature of the flammable vapors. The results were obtained by experimentnal and by computational fluid dynamics.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 80
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 60 (1998), S. 204-215 
    ISSN: 0006-3592
    Keywords: immobilization ; white-rot fungi ; Lentinula edodes ; manganese peroxidase ; Mn3+ ; azlactone ; chlorophenol ; EEDQ ; biocatalyst ; bioremediation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Manganese peroxidase (MnP) purified from commercial cultures of Lentinula edodes was covalently immobilized through its carboxyl groups using an azlactone-functional copolymer derivatized with ethylenediamine and 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline (EEDQ) as a coupling reagent. The tethered enzyme was employed in a two-stage immobilized MnP bioreactor for catalytic generation of chelated MnIII and subsequent oxidation of chlorophenols. Manganese peroxidase immobilized in the enzyme reactor (reactor 1) produced MnIII-chelate, which was pumped into another chemical reaction vessel (reactor 2) containing the organopollutant. Reactor 1-generated MnIII-chelates oxidized 2,4-dichlorophenol and 2,4,6-trichlorophenol in reactor 2, demonstrating a two-stage enzyme and chemical system. H2O2 and oxalate chelator concentrations were varied to optimize the immobilized MnP's oxidation of MnII to MnIII. Oxidation of 1.0 mM MnII to MnIII was initially measured at 78% efficiency under optimized conditions. After 24 h of continuous operation under optimized reaction conditions, the reactor still oxidized 1.0 mM MnII to MnIII with ∼69% efficiency, corresponding to 88% of the initial MnP activity. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 204-215, 1998.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 81
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 482-494 
    ISSN: 0006-3592
    Keywords: polycyclic aromatic hydrocarbon ; biodegradation ; surfactants ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The objectives of this study were to isolate and evaluate microorganisms with the ability to degrade high molecular weight polycyclic aromatic hydrocarbons (PAHs) in the presence of synthetic surfactants. Stenotrophomonas maltophilia VUN 10,010, isolated from PAH-contaminated soil, utilized pyrene as a sole carbon and energy source and also degraded other high molecular weight PAHs containing up to seven benzene rings. Various synthetic surfactants were tested for their ability to improve the PAH degradation rate of strain VUN 10,010. Anionic and cationic surfactants were highly toxic to this strain, and the Tween series was used as a growth substrate. Five nonionic surfactants (Brij 35, Igepal CA-630, Triton X-100, Tergitol NP-10, and Tyloxapol) were not utilized by, and were less toxic to, strain VUN 10,010. MSR and log Km values were determined for fluoranthene, pyrene, and benzo[a]pyrene in the presence of these nonionic surfactants and their apparent solubility was increased by a minimum of 250-fold in the presence of 10 g L-1 of all surfactants. The rate of pyrene degradation by strain VUN 10,010 was enhanced by the addition of four of the nonionic surfactants (5-10 g L-1); however, 5 g L-1 Igepal CA-630 inhibited pyrene degradation and microbial growth. The specific growth rate of VUN 10,010 on pyrene was increased by 67% in the presence of 10 g L-1 Brij 35 or Tergitol NP-10. The addition of Brij 35 and Tergitol NP-10 to media containing a single high molecular weight PAH (four and five benzene rings) as the sole carbon source increased the maximum specific PAH degradation rate and decreased the lag period normally seen for PAH degradation. The addition of Tergitol NP-10 to VUN 10,010 cultures which contained a PAH mixture (three to seven benzene rings) substantially improved the overall degradation rate of each PAH and increased the specific growth rate of VUN 10,010 by 30%. Evaluation of the use of VUN 10,010 for degrading high molecular weight PAHs in leachates from surfactant-flushed, weathered, PAH-contaminated sites is warranted. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:482-494, 1998.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 82
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 345-355 
    ISSN: 0006-3592
    Keywords: cyclodextrin ; polychlorobiphenyl ; chlorobenzoic acid ; soil ; bioremediation ; biodegradation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The possibility of enhancing the intrinsic ex-situ bioremediation of a chronically polychlorinated biphenyl-contaminated soil by using cyclodextrins was studied in this work. The soil, contaminated with a large array of polychlorinated biphenyls and deriving from a dump site where it has been stored for about 10 years, was found to contain indigenous cultivable aerobic bacteria capable of utilising biphenyl and chlorobenzoic acids. The soil was amended with inorganic nutrients and biphenyl, saturated with water, and treated in aerobic batch slurry- and fixed-phase reactors. Hydroxypropyl-β-cyclodextrin and γ-cyclodextrin, added to both reactor systems at the concentration of 10 g/L at the 39th and 100th days of treatment, were found to generally enhance the depletion rate and extent of the soil polychlorobiphenyls. Despite some abiotic losses could have affected the depletion data, experimental evidence, such as the production of metabolites tentatively characterized as chlorobenzoic acids and chloride ion accumulation in the reactors, indicated that cyclodextrins significantly enhanced the biological degradation of the soil polychlorobiphenyls. This result has been ascribed to the capability of cyclodextrins of enhancing the availability of polychlorobiphenyls in the hydrophilic soil environment populated by immobilised and suspended indigenous soil microorganisms. Both cyclodextrins were metabolised by the indigenous soil microorganisms at the concentration at which they were used. Therefore, cyclodextrins, both for their capability of enhancing the biodegradation of soil polychlorobiphenyls and for their biodegradability, can have the potential of being successfully used in the bioremediation of chronically polychlorinated biphenyl-contaminated soils. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:345-355, 1998.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 83
    ISSN: 0006-3592
    Keywords: cellulose ; gel ; fiber ; immobilization ; adsorption ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We prepared a new composite gel fiber by the gel formation of cellulose acetate and titanium iso-propoxide. The fiber is harder than alginate gel; it is also stable in common solvents, phosphate solution, and electrolyte solutions over a wide range of pH from 3 to 10. The fiber shows amphoretic adsorption properties depending on pH, namely, it acts anionic with decreasing pH and cationic with increasing pH. However, the fiber had no adsorption property for a pyrogen endotoxin. The β-galactosidase and α-chymotrypsin not retained in alginate gel were immobilized on the fibers by this method. The pH, temperature, and repeated run stabilities of the immobilized enzyme were compared to those of the native one. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:651-656, 1998.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 84
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 393-399 
    ISSN: 0006-3592
    Keywords: denitrification ; biodegradation ; kinetics ; 1,1,1-trichloroethane ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A denitrifying consortium capable of degrading carbon tetrachloride (CT) was shown to also degrade 1,1,1-trichloroethane (TCA). Fed-batch experiments demonstrated that the specific rate of TCA degradation by the consortium was comparable to the specific rate of CT degradation (approximately 0.01 L/gmol/min) and was independent of the limiting nutrient. Although previous work demonstrated that 4-50% of CT transformed by the consortium was converted to chloroform (CF), no reductive dechlorination products were detected during TCA degradation, regardless of the limiting nutrient. The lack of chlorinated TCA degradation products implies that the denitrifying consortium possesses an alternate pathway for the degradation of chlorinated solvents which does not involve reductive dechlorination. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:393-399, 1998.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 85
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 60 (1998), S. 397-407 
    ISSN: 0006-3592
    Keywords: nonionic surfactants ; mass transfer ; bioavailability ; PAH ; biodegradation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of six nonionic surfactants, Igepal CA-720, Tergitol NPX, Triton X-100, PLE4, PLE10, and PLE23, on the dissolution rate of solid naphthalene was studied in stirred batch reactors. Results showed increased mass-transfer rates with increased surfactant concentrations up to 10 kg m-3. Dissolution experiments were adequatly described by a mechanistic mass-transfer model. Partitioning of naphthalene into the micelles and the diffusion coefficients of the micelles affected the dissolution rate most significantly. Combined dissolution and biodegradation experiments with Triton X-100 or PLE10 with naphthalene showed that the biomass-formation rate of Pseudomonas 8909N (DSM No. 11634) increased concomitantly with the mass-transfer rate under naphthalene-dissolution limited conditions up to surfactant concentrations of 6 kg m-3. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 397-407, 1998.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 86
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. S3 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 87
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 9-15 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design and deflagration pressure relief vents is based on correlations developed for various types of combustible materials and for enclosures of different strengths. The primary guideline for deflagration vent design in the US is NFPA 68 Guide for Venting of Deflagrations [5]. That document gives guidance for the design of vents for enclosures containing flammable gases, specifically hydrogen, coke oven gas, propane, and methane. Application of the guide to other gases is achieved using the KG value. Values of KG are published for a relatively small number of gases, as seen in Table D-1 of NFPA 68. This work present KG data on several additional gases obtained in a laboratory scale test vessel along with analysis of the results with respect to published values of fundamental burning velocity.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 88
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 23-31 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In May 1996, the Flammable and Combustible Liquids Code Committee of the National Fire Protection Association (NFPA) proposed for adoption by the Association a new edition of NFPA 30, Flammable and Combustible Liquids Code. This new edition was the culmination of two and one-half years' work by the Committee and included one of the most significant changes to that document in some twenty years: the incorporation of mandatory fire protection criteria for warehouses and other inside areas that store flammable and combustible liquids in containers and portable tanks.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 89
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 32-38 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper describes the development of a risk ranked Inspection Recommendation procedure that is used by one of Exxon's chemical plants to prioritize repairs that have been identified during equipment inspection.As part of the Company's Safety Management Practices initiative in the late 1980's a procedure was put into place to ensure that an Inspector's repair recommendations were properly addressed by the organization. The initial procedures were successful at “systematizing” the documentation and stewardship-to-completion of the Inspector's recommendation, however, there were complications with the original process: (1)The Inspector made a simple High, Medium or Low assessment of the priority/criticality of the recommendation. Frequently, this resulted in disagreements with Operations about the true priority of the recommendation.(2)If there was agreement on the priority of the recommendation, there was still disagreement on the relative rank within the priority-which high priority was the highest priority?(3)With limited funds to spend on repairs, it was (and is) important to make sure that the money was being spent on the highest risk items that had the greatest risk reduction/cost benefit ratio.To address these concerns, the procedure was modified to incorporate a risk assessment of the recommendation by both the Inspector and Operations. In the new procedure, the Inspector describes the deficiency that he/she finds and assesses the probability of failure within a certain time-frame. Operations must assess the consequences, from an environmental, safety and economics standpoint, were the failure to occur. These assessments are combined in the typical risk equation (risk = probability × consequences) to arrive at a severity index which serves to rank the recommendation relative to the other recommendations. Because Operations participates in the assessment there is very little disagreement about the priority of the recommendation. The severity index puts the recommendations in order so it is quite clear which are the highest priority recommendations. This process has helped to focus the entire organization on those deficiencies that represent the greatest risk with the result that less time and money is spent correcting items that have a low risk/cost benefit ratio, allowing these savings to be used to reduce the higher risks in the plant.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 90
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 124-126 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simple analytical method is presented for estimating the hybrid minimum ignition energy (HMIE) of dust-gas mixtures, based on the assumed generality of Bartknecht's well-known test data for mixtures of propane with a series of dusts in air. Since the HMIE equation requires input data which might be unavailable, the use of conservative default methods is discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 91
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 138-148 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A large and potentially hazardous decrease in aldehyde autoignition temperature (AIT) occurs with increased pressure. The AIT-pressure curve determined in a 5 L stainless steel sphere was similar for propionaldehyde and butyraldehyde in air, falling from about 185°C at atmospheric pressure to 90°C at 140 psia. Reduction of oxygen concentration had little effect on propionaldehyde AIT. At 100°C and 140 psia, autoignitions accompanied by at least a doubling of pressure were observed above 4% oxygen. In the presence of a few grams of free liquid, propionaldehyde vapor ignited in air at initial conditions significantly below the AIT. The mechanism appears to involve rapid Fe-catalyzed exothermic liquid-phase oxidation leading to autoignition of the adjacent heated gas layer. An acetaldehyde vapor-air mixture in the presence of free liquid and rust exploded at room temperature when air pressure was increased to 95 psia; this result is discussed with reference to a cylinder overpressurization that occurred while making up an ostensibly sub-LFL calibration mixture with compressed air. Propionaldehyde's limiting oxygen concentration (LOC) was investigated in the near-autoignition region using the same 5L apparatus; the findings are discussed with reference to an overpressurization incident in an air-liquid partial oxidation reactor. The general results are used to illustrate the application of LOC in partial oxidation processes subject to autoignition and to discuss elements of the current ASTM draft test method for LOC, which does not address test difficulties associated with condensable and/or reactive gas systems.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 92
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. F3 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 93
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 157-170 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Risk analysis in chemical process industries is an elaborate exercise involving several steps from preliminary hazard identification to development of credible accident scenarios, to preparation of strategies for prevention or control of damage.All this requires substantial inputs of time and money. In order to get an approximate yet workable assessment of risk at much lesser costs, indices have been developed which link typical findings of elaborate risk analysis to scales of risk. The scales, in turn, provide workable measures of hazards/risks/safety.In the past, indices have been reported for swift risk assessment - the noteworthy among them include Dow fire and explosion index, Mond fire, explosion and toxicity index, IFAL index, and mortality index. A few rapid ranking techniques have also been proposed.This paper presents a new system of methodologies for Hazard Identification and Ranking (HIRA). The system consists of two indices: one for fire and explosion hazards and another for the hazard due to likely release of toxic chemical. The magnitudes of these indices indicate the severity of the likely accident; in terms of the size of the impacted area.HIRA has been applied to a typical chemical process industry - a sulfolane plant - and its performance has been compared with that of the Dow's and the Mond's indices. The study reveals that HIRA is more sensitive and accurate than the other indices.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 94
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 200-208 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 95
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 213-218 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: On October 31, 1987 a crane lifting a heat exchanger convection section failed and severed a 4″ loading line and a 2″ pressure relief line to an HF alkylation reactor settler drum at a petroleum refinery in Texas City, Texas. Vapors were emitted under pressure for about two hours and the vessel was plugged and drained aproximately 44 hours later. A plume from this accidental release passed through residential areas, damaging some vegetation (brown lawns), and spawning a class action law suit. An extensive analysis was conducted to determine the total inventory loss and to model the blowdown process and the concentrations of HF in the plume. Since the discharge rate was decreasing with time, a peak concentration of HF in the emitted vapors occurred just before the water spray mitigation system became fully operative. Consequently, the mitigation efforts were more effective late in the response when concentrations were already low. The predicted plume concentrations are consistent with observed vegetation damage effects, with concentrations below Emergency Response Planning Guideline Level 3 past 3/4 mile from the source. These results support a policy of sheltering in place during such an event.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 96
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A fifty-five gallon steel drum of a liquid organic peroxide pressurized and ruptured in the mix room of a manufacturing plant. The head of the drum blew off and the ejected material ignited. The resulting fire was extinguished by the building sprinkler system and operating personnel. Although there were no injuries, the fire caused significant damage in the mix room. The investigation of this incident, its likely cause, and the corrective actions will be discussed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 97
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 238-242 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The Brazoria County Petrochemical Council, 13 companies that are working together to enhance relations between industry and the community, united in a joint effort at complying with the EPA's Risk Management Program. One of the significant issues the group had to address was the need to develop meaningful hazard assessment for presentation to the public. The EPA's “Table Look-Up Approach” found in the Offsite Consequence Analysis Guidance document is certainly a good tool; however, the built-in conservatism results in over-estimates of potential hazard areas. Much more meaningful results are shown to be obtained using one of the hazard release models.The value of using a credible scenario with realistic meteorological data is demonstrated through the consistently smaller areas predicted by the PHAST Model for planning purposes. Realistic scenarios/failure modes and realistic model parameters are important so that the risk to the public is not overstated. Proprietary models such as PHAST are invaluable in providing more meaningful consequences for planning purposes.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 98
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 263-271 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some of the hazards encountered by process plant operators involve the operation of in-line valves to control, start, and to stop flow. Torque required to operate valves may vary according to valve wheel size, in-line pressure, and valve flange position (open/closed). This study determined how valve wheel size, in-line pressure and valve position (open/closed) affect torque required to actuate a valve. Data were gathered with each combination of size, pressure and position for 336 valves in an operating petrochemical process facility. The results indicate that the main effects of valve wheel size, the in-line pressure, and open/closed valve position significantly affect operational torque requirements. In addition, the interaction between position and pressure was significant for operational torque. The implication of these results is that operators are exposed to operational torque requirements that exceed maximum acceptable capabilities that have been determined in previous studies.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 99
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 288-296 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An ammonia storage tank was built at the BASF Antwerp site in 1969 on land reclaimed from the sea. After several years of operation uneven foundation settlement, of up 2, occurred. In order to assure stability of this area for the next operation period (at least 10 years) measures were taken to ensure continued safe operation. One key measure was strain gauge monitoring at the location of maximum stress.
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 100
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 17 (1998), S. 297-301 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The Baker-Strehlow methodology was developed to provide an objective approach to prediction of blast pressures from vapor cloud explosions. The complete methodology was first published in 1994 [1]. Since then, it has evolved through ongoing research and use in VCE hazard analyses, facility siting studies and accident investigations. This article gives a brief overview of a paper on recent developments in the Baker-Strehlow methodology presented at the 31st Loss Prevention Symposium in Houston on March 9-13, 1997. Because the entire paper is too lengthy to be presented here, the following discussions may be lacking in some details. A copy of the complete paper can be obtained from the American Institute of Chemical Engineers (AIChE).Since the Baker-Strehlow method was first published, it has been used extensively in VCE hazard assessments in refineries and chemical plants. As expected, many practical lessons have been learned during the course of the hazard assessments, and the Baker-Strehlow method has evolved as a result. The changes have been evolutionary, not revolutionary. In keeping with the goals of the original study in which the methodology was developed, all changes have been incorporated with the intent of achieving an objective methodology to provide consistent prediction of VCE blast effects.The revisions to the Baker-Strehlow method resulting from experience gained during plant walk-downs and hazard assessments include: Systematic identification of “potential explosion sites” or “PESs,”Selection of the level of confinement for mixed zones of 2D and 3D confinement,Deciding on flame expansion when confinement is elevated above the vapor cloud,Selecting the reactivity for a fuel that is a mixture of fuels with differing reactivities,Predicting blast loads when there are multiple PES's within a vapor cloud considering different ignition source locations.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...