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  • Articles  (30)
  • Animals
  • Chemical Engineering
  • Electronic structure and strongly correlated systems
  • adaptation
  • temperature
  • Springer  (30)
  • Geography  (18)
  • Process Engineering, Biotechnology, Nutrition Technology  (12)
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  • Articles  (30)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 297-301 
    ISSN: 1573-0972
    Keywords: Anaerobic bacteria ; growth ; protease ; psychrotrophs ; temperature ; volatile fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Five anaerobic proteolytic bacteria were isolated from water bodies of Leh, India, where the ambient temperature varies from −25 to 25 °C. Isolates showed growth at all temperatures ranging from 5 to 37 °C except SPL-4 and SPL-5 which showed no growth at 5 °C. The cultures could grow and produce proteases on various protein substrates and the yield varied with the substrates. Two of the cultures showed the presence of spores. Acetate was the dominant VFA during hydrolysis of protein substrates.
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  • 2
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    World journal of microbiology and biotechnology 16 (2000), S. 571-572 
    ISSN: 1573-0972
    Keywords: Anaerobes ; hydrogen sulphide ; rubber stoppers ; sulphate reduction ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Common black rubber stoppers, made from natural rubber and styrene–butadiene, may cause a loss of hydrogen sulphide from aqueous media and impede the growth of sulphate-reducing bacteria under thermophilic conditions.
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  • 3
    ISSN: 1573-0972
    Keywords: Carbondioxide ; fungi ; oxygen ; Rhizopus ; solid-substrate fermentation SSF ; tempe modelling ; temperature ; water activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Rhizopus microsporus var. microsporus and var. oligosporus are used in the manufacture of various Asian fermented foods (tempe, black oncom, sufu). In view of solid-substrate fermentation (SSF) control, mycelial growth of strains of both varieties was tested for sensitivity to fluctuations of temperature, water activity and interstitial gas composition. This was achieved by measuring radial growth as well as biomass dry weight of pre-germinated microcolonies on defined media. The optimum conditions were temperature 40 °C, a w 0.995 and a gas composition of air for the growth of both strains on a model medium. Whereas radial growth rates of var. microsporus and var. oligosporus were similar, biomass growth rates of var. oligosporus were higher than those of var. microsporus under optimum conditions. The temperature-dependent growth of Rhizopus spp. at a w 〉 0.98 could be described by the Ratkowsky Equation. Carbon dioxide (5–10% v/v) inhibited the growth of Rhizopus spp. at non-limiting levels of oxygen. The two strains were able to grow at low (0.5% v/v) oxygen levels, but the mycelial density was rather low. No interrelation of water activity and gas composition was observed, but at high water activity the fungi were more sensitive to changes of temperature. The implications for process control are discussed.
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  • 4
    Electronic Resource
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    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 607-612 
    ISSN: 1573-0972
    Keywords: Aflatoxin ; apple ; fruit oils ; fungi ; patulin ; sodium hypochlorite ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Aspergillus flavus, A. niger, Penicillium expansum and Rhizopus stolonifer were the most frequently isolated fungi from healthy apple fruits. Alternaria alternata was the most common organism of rotten apple fruits, followed by A. niger, A. flavus, P. expansum and R. stolonifer. The prevalent type of decay, brown rot lesion, is caused by R. stolonifer followed by A. flavus, A. niger, A. alternata and P. expansum. Sodium hypochlorite had good curative properties against fruit rots. The main natural mycotoxins produced in rotten apple were patulin and aflatoxins. The optimum temperature for patulin production by P. expansum was 15 °C after 15 days. Complete inhibition of patulin formation was attained using 0.2% lemon oil and 〉 90% inhibition using 0.05% lemon and 0.2% orange oils. Also significant inhibition (〉 90%) of aflatoxin production was observed with 0.2% lemon oil.
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  • 5
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    World journal of microbiology and biotechnology 15 (1999), S. 335-338 
    ISSN: 1573-0972
    Keywords: Anaerobic digestion ; biogas ; pathogens ; survival ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The survival of certain pathogenic bacteria was studied in anaerobic batch digesters at room temperature (18–25 °C) as well as at 35 °C under laboratory conditions. The survival of Escherichia coli and Salmonella typhi at room temperature was upto 20 days whereas at 35 °C it was only upto 10 days. Shigella dysenteriae was found to be the most sensitive organism which could survive upto 10 days at room temperature and upto 5 days at 35 °C. The longest survival was observed in case of Streptococcus faecalis which could survive upto 35 days at room temperature and 15 days at 35 °C. The survival time of Salmonella typhi increased when the solid contents of the digester were elevated from 9% to 15%.
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  • 6
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    Mitigation and adaptation strategies for global change 4 (1999), S. 25-41 
    ISSN: 1573-1596
    Keywords: adaptation ; agriculture ; climate change ; Kazakhstan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Agriculture in Kazakhstan is sensitive to climate, and wheat yields could be reduced up to 70% under climate change. With the transition from a socialist economy to a free market economy, decisions are being made now that will affect Kazakhstan's ability to cope with climate change. A team of Kazakh and American researchers examined the cost-effectiveness and barriers to implementations of adaptation options for climate change. Twelve adaptation options that increase flexibility to respond to climate change were identified using a screening matrix. Four options, forecasting pest outbreaks, developing regional centers for preserving genetic diversity of seeds, supporting a transition to a free market, and reducing soil erosion through the use of changed farming practices, were examined. The Adaptation Decision Matrix (ADM) was then applied to estimate benefits using expert judgment (using an arbitrary numerical scale, not monetary values) and benefits estimates were compared to costs to determine cost-effectiveness. The ADM uses subjective measures of how well adaptation options meet policy objectives. Controlling soil erosion was estimated to have the highest benefits, but the high costs of implementation appears to make it relatively cost-ineffective. Supporting a transition to a free market was ranked as the most cost-effective measure, with regional centers second. However, use of different scales to quantify benefits or different weights can result in regional centers being more cost-effective than the transition to a free market. Regional centers was also judged to have fewer barriers to implementation than a transition to a free market. These results will be incorporated in Kazakhstan's National Action Plan. The ADM and other tools are relatively easy to apply, but are quite subjective and difficult to evaluate. The tools can be quite useful by decision makers to analyze advantages and disadvantages between different adaptation options, but should be supplemented with additional, particularly quantitative analysis.
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  • 7
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    Mitigation and adaptation strategies for global change 4 (1999), S. 137-165 
    ISSN: 1573-1596
    Keywords: adaptation ; agriculture ; climate change ; decision-making ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This work presents a framework for viewing agricultural adaptation, emphasizing the multiple spatial and temporal scales on which individuals and institutions process information on changes in their environment. The framework is offered as a means to gain perspective on the role of climate variability and change in agricultural adaptation, and developed for a case study of Australian agriculture. To study adaptation issues at the scale of individual farms we developed a simple modelling framework. The model highlights the decision making element of adaptation in light of uncertainty, and underscores the importance of decision information related to climate variability. Model results show that the assumption of perfect information for farmers systematically overpredicts adaptive performance. The results also suggest that farmers who make tactical planting decisions on the basis of historical climate information are outperformed by those who use even moderately successful seasonal forecast information. Analysis at continental scales highlights the prominent role of the decline in economic operating conditions on Australian agriculture. Examples from segments of the agricultural industry in Australia are given to illustrate the importance of appropriate scale attribution in adapting to environmental changes. In particular, adaptations oriented toward short time scale changes in the farming environment (droughts, market fluctuations) can be limited in their efficacy by constraints imposed by broad changes in the soil/water base and economic environment occuring over longer time scales. The case study also makes the point that adaptation must be defined in reference to some goal, which is ultimately a social and political exercise. Overall, this study highlights the importance of allowing more complexity (limited information, risk aversion, cross-scale interactions, mis-attribution of cause and effect, background context, identification of goals) in representing adaptation processes in climate change studies.
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  • 8
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    Mitigation and adaptation strategies for global change 4 (1999), S. 199-213 
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; impact assessment ; response options ; vulnerability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper outlines what is meant by "adaptation" to climate change, and how it might be addressed in the IPCC Assessments. Two roles of adaptation in the climate change field are identified: adaptation as part of impact assessment (where the key question is: what adaptations are likely?), and adaptation as part of the policy response (where the central question is: what adaptations are recommended?). The concept of adaptation has been adopted in several fields including climate impact assessment and policy development, risk management, and natural hazards research. A framework for systematically defining adaptations is based on three questions: (i) adaptation to what? (ii) who or what adapts? and (iii) how does adaptation occur? The paper demonstrates that, for adaptation purposes, climate extremes and variability are integral parts of climate change, along with shifts in mean conditions. Attributes for differentiating adaptations include purposefulness, timing, temporal and spatial scope, effects, form and performance. The framework provides a guide for the treatment of adaptation in the IPCC assessments, both in the assessment of impacts and in the evaluation of adaptive policy options.
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  • 9
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    Mitigation and adaptation strategies for global change 4 (1999), S. 215-225 
    ISSN: 1573-1596
    Keywords: climate ; adaptation ; impacts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract There is a critical need to collectively understand, to develop adaptation options to enhance the benefits, and to reduce the social and economic vulnerabilities induced by climate variability and change. This paper uses key questions to help build a framework for adaptation by first organizing the questions into adaptation science, management and option components, including their respective sub-categories. The process of adaptation depends on many factors, including who or what adapts, what they adapt to, how they adapt and what and how resources are used. This conceptual model is designed to organize concepts regarding adaptation, to help stimulate ideas, and to explore the linkages among parts of the adaptation cycle. Predictive models need to be developed to determine the outcomes of planned adaptation strategies. For the best and most realistic evaluation of climate problems, adaptation and impacts should be considered together. This joint approach improves the assessment of the significance and dangers of the current and future climate, as well as the determination of solutions (e.g., how to prepare for a changing climate) and their priorities. Challenges of adaptive management are discussed in terms of a framework with linkages to adaptation science and options. Adaptation research and applications work continue to build on the foundation of science and management frameworks to address the risks and uncertainties in the decision-making process and to identify adaptation options.
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  • 10
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    Mitigation and adaptation strategies for global change 4 (1999), S. 227-237 
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; climate variability ; data ; climate applications ; El Niño ; UNFCCC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract An extensive foundation of high quality data and information on the climate and on the biological, environmental and social systems affected by climate is required in order to understand the climate impact processes involved, to develop new adaptation practices, and to subsequently implement these practices. Experience of the impacts of current and past variability of climate and sea level is a prime source of information. Many practices are in use to reduce climate impacts, for example in engineering design, agricultural risk management and climate prediction services, though their roles as adaptations to climate change are not widely appreciated. While there are good data sets on some factors and in some regions, in many cases the databases are inadequate and there are few data sets on adaptation-specific quantities such as vulnerability, resilience and adaptation effectiveness. Current international action under the United Nations Framework Convention on Climate Change (UNFCCC) pays little attention to adaptation and its information requirements. Furthermore there are trends toward reduced data gathering and to restrictions on access to data sets, especially arising from cost and commercialisation pressures. To effectively respond to the changes in climate that are now inevitable, governments will need to more clearly identify adaptation as a central feature of climate change policy and make a renewed shared commitment to collecting and freely exchanging the necessary data.
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  • 11
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    Mitigation and adaptation strategies for global change 4 (1999), S. 239-252 
    ISSN: 1573-1596
    Keywords: climate change ; coastal zones ; adaptation ; vulnerability ; IPCC Technical Guidelines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper evaluates the IPCC Technical Guidelines for Assessing Climate Change Impacts and Adaptations with respect to the guidance offered for coastal-adaptation assessment. It appears that the IPCC Technical Guidelines focus strongly on implementation. This paper uses both conceptual and empirical information is used in this paper to show that coastal adaptation embraces more than selecting one of the "technical" options to respond to sea-level rise (retreat, accommodate or protect). Coastal adaptation is a more complex and iterative process with a series of policy cycles. To be effective, an expanded adapta-tion framework involving four steps is suggested, including (i) information collection and awareness raising; (ii) planning and design; (iii) implementation; and (iv) monitoring and evaluation. The incom-plete coverage of these four steps in existing coastal-adaptation assessments constrains the development of adaptation strategies that are supported by the relevant actors and integrated into existing management. Researchers and policy-makers are recommended to work together to establish a framework for adaptation that is integrated within current coastal management processes and practices and takes a broader view on the subject.
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  • 12
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    Mitigation and adaptation strategies for global change 4 (1999), S. 319-329 
    ISSN: 1573-1596
    Keywords: uncertainty ; risk ; adaptation ; extreme events ; (credible) information ; integrated assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper draws ten lessons from analyses of adaptation to climate change under conditions of risk and uncertainty: (1) Socio-economic systems will likely respond most to extreme realizations of climate change. (2) Systems have been responding to variations in climate for centuries. (3) Future change will effect future citizens and their institutions. (4) Human systems can be the sources of surprise. (5) Perceptions of risk depend upon welfare valuations that depend upon expectations. (6) Adaptive decisions will be made in response to climate change and climate change policy. (7) Analysis of adaptive decisions should recognize the second-best context of those decisions. (8) Climate change offers opportunity as well as risk. (9) All plausible futures should be explored. (10) Multiple methodological approaches should be accommodated. These lessons support two pieces of advice for the Third Assessment Report: (1) Work toward consensus, but not at the expense of thorough examination and reporting of the "tails" of the distributions of the future. (2) Integrated assessment is only one unifying methodology; others that can better accommodate those tails should be encouraged and embraced.
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  • 13
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    Mitigation and adaptation strategies for global change 4 (1999), S. 307-318 
    ISSN: 1573-1596
    Keywords: adaptation ; climatic change ; economic welfare ; costs and benefits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential damages of climate change and climate variability are dependent upon the responses or adaptations that people make to their changing environment. By adapting the management of resources, the mix and methods of producing goods and services, choices of leisure activities, and other behavior, people can lessen the damages that would otherwise result. A framework for assessing the benefits and costs of adaptation to both climate change and climate variability is described in the paper. The framework is also suitable for evaluating the economic welfare effects of climate change, allowing for autonomous adaptation by private agents. The paper also briefly addresses complications introduced by uncertainty regarding the benefits of adaptation and irreversibility of investments in adaptation. When investment costs are irreversible and there is uncertainty about benefits, the usual net present value criterion for evaluating the investment gives the wrong decision. If delaying an adaptation project is possible, and if delay will permit learning about future benefits of adaptation, it may be preferable to delay the project even if the expected net present value is positive. Implications of this result for adaptation policy are discussed in the paper.
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  • 14
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    Mitigation and adaptation strategies for global change 4 (1999), S. 343-361 
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; socioeconomic impacts ; Egypt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Assessment of the vulnerability and expected socioeconomic losses over the Nile delta coast due to the impact of sea level rise is carried out in details. Impacts of sea level rise over the Governorates of Alexandria and Port Said in particular, are evaluated quantitatively. Analysis of the results at Alexandria Governorate indicate that, if no action is taken, an area of about 30% of the city will be lost due to inundation. Almost 2 million people will have to abandon their homeland; 195,000 jobs will be lost and an economic loss of over $3.5 Billion is expected over the next century. At Port Said Governorate results indicate that beach areas are most severely affected (hence tourism), followed by urban areas. The agriculture sector is the least affected sector. It is estimated that the economic loss is over $ 2.0 Billion for 0.50 m SLR and may exceed $ 4.4 Billion for 1.25 m SLR. Options and costs of adaptation are analyzed and presented. Multi-criteria and decision matrix approaches, based on questionnaire surveys are carried out to identify priorities for the two cases. Analysis of these techniques of two options; the current policy (hard protection measures on some vulnerable areas) and no action (stopping these activities) have the lowest scores. Beach nourishment and integrated coastal zone management (ICZM) have the highest scores, however ICZM has high cost measures. The most cost-effective option is the land-use change, however with relatively very high cost measure. It is recommended that an ICZM approach be adopted since it provides a reasonable trade off between costs and cost effectiveness.
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  • 15
    ISSN: 1573-0778
    Keywords: adaptation ; antibody production rate ; hybridoma ; intracellular amino acids ; osmotic pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The time length required for the adaptation of AFP-27 hybridoma cells to high osmotic pressure and the effect of a gradual increase of osmotic pressure on monoclonal antibody production were investigated. When the cells were subjected to an increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg- 1, the intracellular content of osmoprotective free amino acids reached a maximum level 6 h after the osmotic pressure was increased to 366 mOsmol kg-1. The same time period of 6 h incubation at 366 mOsmol kg-1 was required to obtain a high growth rate of AFP-27 cells at 440 mOsmol kg-1 when the cells were subjected to a two-step increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg-1 and then to 440 mOsmol kg-1. The time length for the physiological adaptation of the cells to 366 mOsmol kg-1 was consequently estimated to be 6 h. Osmotic pressure during batch cultivation was gradually increased from 300 mOsmol kg-1 to 400 mOsmol kg-1 with an adaptation time of at least 6 h. The specific growth rates following a gradual increase of osmotic pressure were higher than those at a constant osmotic pressure of 400 mOsmol kg-1, while the specific monoclonal antibody production rate increased with the increase in the mean osmotic pressure. As a result, the cells grown under a gradual increase of osmotic pressure produced higher amounts of monoclonal antibodies than did those grown under constant osmotic pressure.
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  • 16
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    World journal of microbiology and biotechnology 14 (1998), S. 847-850 
    ISSN: 1573-0972
    Keywords: Kinema ; soybean ; Bacillus subtilis KK2:B10 ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Kinema was prepared by fermenting whole cooked soybeans with pure culture of Bacillus subtilis KK2:B10 (MTCC 2756) strain at 35°C, 40°C and 45°C for 24h. Temperature, mesophilic plate counts, relative viscosity, water-soluble nitrogen, formal nitrogen contents and reducing sugars of fermenting soybeans were investigated during fermentation. At higher temperatures the growth rate of B. subtilis KK2:B10 was faster. A remarkable increase in the relative viscosity of kinema was observed at 40°C during fermentation. Water-soluble nitrogen and formol nitrogen to total nitrogen contents increased throughout the 24h of fermentation. Reducing sugars increased during the log phase and then decreased sharply. Kinema matured below 10°C for 1 day after the desired fermentation showed a significant increase in relative viscosity. The quality of kinema was maintained with pure culture fermentation by B. subtilis KK2:B10 at 40°C for 20h and matured at 5°C for 1 day.
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  • 17
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    GeoJournal 45 (1998), S. 77-83 
    ISSN: 1572-9893
    Keywords: globalization ; national tradition ; Russian human geography inertia ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
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  • 18
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    Mitigation and adaptation strategies for global change 2 (1997), S. 19-44 
    ISSN: 1573-1596
    Keywords: adaptation ; Africa ; agriculture ; climate change ; vulnerability ; water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The intersection of present vulnerability and the prospect of climate change in Africa warrants proactive action now to reduce the risk of large-scale, adverse impacts. The process of planning adaptive strategies requires a systematic evaluation of priorities and constraints, and the involvement of stakeholders. An overview of climate change in Africa and case studies of impacts for agriculture and water underlie discussion of a typology of adaptive responses that may be most effective for different stakeholders. The most effective strategies are likely to be to reduce present vulnerability and to enhance a broad spectrum of capacity in responding to environmental, resource and economic perturbations. In some cases, such as design of water systems, an added risk factor should be considered.
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  • 19
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    Mitigation and adaptation strategies for global change 2 (1997), S. 19-44 
    ISSN: 1573-1596
    Keywords: adaptation ; Africa ; agriculture ; climate change ; vulnerability ; water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The intersection of present vulnerability and the prospect of climate change in Africa warrants proactive action now to reduce the risk of large-scale, adverse impacts. The process of planning adaptive strategies requires a systematic evaluation of priorities and constraints, and the involvement of stakeholders. An overview of climate change in Africa and case studies of impacts for agriculture and water underlie discussion of a typology of adaptive responses that may be most effective for different stakeholders. The most effective strategies are likely to be to reduce present vulnerability and to enhance a broad spectrum of capacity in responding to environmental, resource and economic perturbations. In some cases, such as design of water systems, an added risk factor should be considered.
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  • 20
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    Mitigation and adaptation strategies for global change 1 (1997), S. 251-271 
    ISSN: 1573-1596
    Keywords: Greece ; maize ; climate change ; CO2 effects ; adaptation ; crop simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential impacts of climate change on the phenology and yield of two maize varieties in Greece were studied. Three sites representing the central and northern agricultural regions were selected: Karditsa, Naoussa and Xanthi. The CERES-Maize model, embedded in the Decision Support System for Agrotechnology Transfer (DSSAT 3.0), was used for the crop simulations, with current and possible future management practices. Equilibrium doubled CO2 climate change scenarios were derived from the GISS, GFDL, and UKMO general circulation models (GCMs); a transient scenario was developed from the GISS GCM transient run A. These scenarios predict consistent increases in air temperature, small increases in solar radiation and precipitation changes that vary considerably over the study regions in Greece. Physiological effects of CO2 on crop growth and yield were simulated. Under present management practices, the climate change scenarios generally resulted in decreases in maize yield due to reduced duration of the growing period at all sites. Adaptation analyses showed that mitigation of climate change effects may be achieved through earlier sowing dates and the use of new maize varieties. Varieties with higher kernel-filling rates, currently restricted to the central regions, could be extended to the northern regions of Greece. In the central regions, new maize varieties with longer grain-filling periods might be needed.
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  • 21
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    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
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  • 22
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    Mitigation and adaptation strategies for global change 1 (1997), S. 233-250 
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Egypt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract If no timely measures are taken to adapt Egyptian agriculture to possible climate warming, the effects may be negative and serious. Egypt appears to be particularly vulnerable to climate change because of its dependence on the Nile River as the primary water source, its large traditional agricultural base, and its long coastline, already undergoing both intensifying development and erosion. A simulation study characterized potential yield and water use efficiency decreases on two reference crops in the main agricultural regions with possible future climatic variation, even when the beneficial effects of increased CO2 were taken into account. On-farm adaptation techniques which imply no additional cost to the agricultural system, did not compensate for the yield losses with the warmer climate or improve the crop water-use efficiency. Economic adjustments such as the improvement of the overall water-use efficiency of the agricultural system, soil drainage and conservation, land management, and crop alternatives are essential. If appropriate measures are taken, negative effects of climate change in agricultural production and other major resource sectors (water and land) may be lessened.
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  • 23
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    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
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  • 24
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    Mitigation and adaptation strategies for global change 1 (1997), S. 251-271 
    ISSN: 1573-1596
    Keywords: Greece ; maize ; climate change ; CO2 effects ; adaptation ; crop simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential impacts of climate change on the phenology and yield of two maize varieties in Greece were studied. Three sites representing the central and northern agricultural regions were selected: Karditsa, Naoussa and Xanthi. The CERES-Maize model, embedded in the Decision Support System for Agrotechnology Transfer (DSSAT 3.0), was used for the crop simulations, with current and possible future management practices. Equilibrium doubled CO2 climate change scenarios were derived from the GISS, GFDL, and UKMO general circulation models (GCMs); a transient scenario was developed from the GISS GCM transient run A. These scenarios predict consistent increases in air temperature, small increases in solar radiation and precipitation changes that vary considerably over the study regions in Greece. Physiological effects of CO2 on crop growth and yield were simulated. Under present management practices, the climate change scenarios generally resulted in decreases in maize yield due to reduced duration of the growing period at all sites. Adaptation analyses showed that mitigation of climate change effects may be achieved through earlier sowing dates and the use of new maize varieties. Varieties with higher kernel-filling rates, currently restricted to the central regions, could be extended to the northern regions of Greece. In the central regions, new maize varieties with longer grain-filling periods might be needed.
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  • 25
    ISSN: 1573-0778
    Keywords: fluidized-bed reactor ; monoclonal antibody ; on-line monitoring ; sample system ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The monoclonal-antibody production of an immobilized hybridoma cell line cultivated in a fluidized-bed reactor was monitored on-line for nearly 900 h. The monoclonal antibody concentration was determined by an immuno affinity-chromatography method (ABICAP). Antibodies directed against the product, e.g. IgG, were immobilized on a micro-porous gel and packed in small columns. After all IgG present in the sample was bound to the immobilized antibodies, unbound proteins were removed by rinsing the column. Elution of the bound antibodies followed and the antibodies were determined by fluorescence. The analytical procedure was automated with a robotic device to enable on-line measurements. The correlation between the on-line determined data and antibody concentrations measured by HPLC was linear. A sampling system was constructed, which was based on a pneumatically actuated in-line membrane valve integrated into the circulation loop of the reactor. Separation of the cells from the sample stream was achieved by a depth filter made of glass-fibre, situated outside the reactor. Rapid obstruction of the filter by cells or cell debris and contamination of the sample system was avoided by intermittent rinsing of the sample system with a chemical solution. The intermittent rinsing of the filter, which had a surface of 4.8 cm2, resulted in an operational capacity of up to 40 samples (1.0 l total sample volume). Both the sampling system and the analytical device functioned without failure during this long-term culture. The culture temperature was varied between 34 and 40 °C. Raising the temperature from 34 up to 37 °C resulted in a simultaneous increase of growth and specific antibody production rate. Specific metabolic rates of glucose, lactate, glutamine and ammonium stayed constant in this temperature range. A further enhancement of temperature up to 40 °C had a negative effect on the growth rate, whereas the specific monoclonal antibody production rate showed a small increase. The other specific metabolic rates also increased in the temperature range between 38 to 40 °C.
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  • 26
    ISSN: 1573-0778
    Keywords: adaptation ; hybridoma ; monoclonal antibody ; protein free medium ; suspension culture ; weaning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, and ethanolamine. No protein or lipid components were added. All three cell lines were weaned off serum using CDSS and a commercially available protein free medium PFHM-II. Data shown here indicated that normally cells took 1–7 weeks to wean off serum and an additional 2–7 weeks to adapt to suspension culture. After adaptation the cells were able to grow well in suspension culture using both protein free media and in the main performed better than serum containing controls. The stability of the three hybridoma cells for antibody production following freeze/thaw procedures and long term subculturing was also tested. All three lines were frozen using our protein free CDSS medium (containing 0.75% bovine serum albumin and 10% dimethyl sulfoxide) in liquid nitrogen for up to one year. Cells thawed from these stocks recovered well and were able to maintain good growth and antibody production characteristics. One line was shown to grow using our protein free CDSS medium in suspension culture for 12 weeks without loss of antibody productivity.
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  • 27
    Electronic Resource
    Electronic Resource
    Springer
    Natural hazards 9 (1994), S. 5-16 
    ISSN: 1573-0840
    Keywords: Fourier transform ; maximum entropy spectral analysis ; precipitation ; temperature ; climatic change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract In the present work, a precipitation and temperature series from Barcelona (Spain) are analysed in order to detect the possible existence of climatic changes or cycles. The analysis is carried out both from the temporal and spectral standpoints. The techniques used range from the classical periodogram and Blackman-Tukey method through to the Maximum Entropy method. The results do not show the existence of climatic cycles, though they do show a clear tendency toward increased precipitation and decreased temperature, since the last years of series.
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  • 28
    Electronic Resource
    Electronic Resource
    Springer
    Cytotechnology 15 (1994), S. 111-116 
    ISSN: 1573-0778
    Keywords: Adherent animal cells ; glucose ; lactate ; productivity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37°C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.
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  • 29
    Electronic Resource
    Electronic Resource
    Springer
    Cytotechnology 7 (1991), S. 103-112 
    ISSN: 1573-0778
    Keywords: adaptation ; ammonia ; hybridoma ; continuous culture ; serum-free medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Using two mouse-mouse hybridoma cell lines, the response to ammonia step and serial changes was investigated in batch and continuous cultures with serum-free medium. The inhibitory effect of ammonia on cell growth depended on the cultivation mode, and differed markedly between cell lines. The cell line, 4C10B6 producing IgG monoclonal antibody against Pseudomonas, showed a high adaptation ability to ammonia. The 4C10B6 cells could grow under ammonia concentration as high as 21 mmol/l NH4Cl with a viability of 80% in the continuous culture with serial increase in ammonia concentration. Whereas, in the batch culture with ammonia step change the cell growth completely ceased at 12 mmol/l NH4Cl. The other cell line, TO-405 producing IgG monoclonal antibody against hepatitis B surface antigen, could not adapt to ammonia, and the cell growth did not occur at 9 mmol/l NH4Cl even under the ammonia serial change.
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  • 30
    ISSN: 1573-0778
    Keywords: adaptation ; ammonia ; glutamine-free ; glutamine synthetase ; Namalva cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Glutamine has been shown to be a preferred energy source for some established cell lines and cancer cells in culture (Kovacevic, 1971; Kovacevic, 1972; Lavietes, 1974). Empirically, glutamine is the most abundant amino acid in most of the culture media developed. The major end product of glutamine metabolism is ammonia. Ammonia build up is one of the limiting factors in the proliferation of mammalian cells in higher density culture and is directly related to the initial glutamine concentration. The susceptibility of glutamine to thermodecomposition prevents the heat sterilization of glutamine-enriched media and this significantly increases the cost of medium preparation at the industrial scale. In an attempt to overcome these drawbacks, a population of Namalva cells capable of growing in glutamine-free media was established. The adapted cells were found to contain a higher level of glutamine synthetase activity which enable them to synthesize sufficient amounts of glutamine for their growth.
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