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  • Articles  (70)
  • adaptation  (31)
  • Bacteria  (30)
  • Animals
  • Chemical Engineering
  • Electronic structure and strongly correlated systems
  • temperature
  • Springer  (70)
  • Process Engineering, Biotechnology, Nutrition Technology  (42)
  • Geography  (18)
  • Ethnic Sciences  (10)
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  • Articles  (70)
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  • 1
    Electronic Resource
    Electronic Resource
    Studies on anaerobic proteolytic bacteria of Leh, India (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 297-301 
    Details
    ISSN: 1573-0972
    Keywords: Anaerobic bacteria ; growth ; protease ; psychrotrophs ; temperature ; volatile fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Five anaerobic proteolytic bacteria were isolated from water bodies of Leh, India, where the ambient temperature varies from −25 to 25 °C. Isolates showed growth at all temperatures ranging from 5 to 37 °C except SPL-4 and SPL-5 which showed no growth at 5 °C. The cultures could grow and produce proteases on various protein substrates and the yield varied with the substrates. Two of the cultures showed the presence of spores. Acetate was the dominant VFA during hydrolysis of protein substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008935425175
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  • 2
    Electronic Resource
    Electronic Resource
    Rubber stoppers used with sulphidic media: a note of caution (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 571-572 
    Details
    ISSN: 1573-0972
    Keywords: Anaerobes ; hydrogen sulphide ; rubber stoppers ; sulphate reduction ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Common black rubber stoppers, made from natural rubber and styrene–butadiene, may cause a loss of hydrogen sulphide from aqueous media and impede the growth of sulphate-reducing bacteria under thermophilic conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008909530445
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of temperature, water activity and gas atmosphere on mycelial growth of tempe fungi Rhizopus microsporus var. microsporus and R. microsporus var. oligosporus (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 853-858 
    Details
    ISSN: 1573-0972
    Keywords: Carbondioxide ; fungi ; oxygen ; Rhizopus ; solid-substrate fermentation SSF ; tempe modelling ; temperature ; water activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Rhizopus microsporus var. microsporus and var. oligosporus are used in the manufacture of various Asian fermented foods (tempe, black oncom, sufu). In view of solid-substrate fermentation (SSF) control, mycelial growth of strains of both varieties was tested for sensitivity to fluctuations of temperature, water activity and interstitial gas composition. This was achieved by measuring radial growth as well as biomass dry weight of pre-germinated microcolonies on defined media. The optimum conditions were temperature 40 °C, a w 0.995 and a gas composition of air for the growth of both strains on a model medium. Whereas radial growth rates of var. microsporus and var. oligosporus were similar, biomass growth rates of var. oligosporus were higher than those of var. microsporus under optimum conditions. The temperature-dependent growth of Rhizopus spp. at a w 〉 0.98 could be described by the Ratkowsky Equation. Carbon dioxide (5–10% v/v) inhibited the growth of Rhizopus spp. at non-limiting levels of oxygen. The two strains were able to grow at low (0.5% v/v) oxygen levels, but the mycelial density was rather low. No interrelation of water activity and gas composition was observed, but at high water activity the fungi were more sensitive to changes of temperature. The implications for process control are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008974621698
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of 2,4-dichlorophenoxyacetic acid-catabolizing bacteria and their biodegradation efficiency in soil (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 495-497 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; biodegradation ; 2,4-D ; soil microcosm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Bacterial isolates (NJ 10 and NJ 15) capable of degrading the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were isolated from agricultural soil by enrichment culture technique. The isolates exhibited substantial growth in mineral salt medium supplemented with 0.1–0.5% of 2,4-D as a sole source of carbon and energy. Based on their morphological, cultural and biochemical characteristics, the isolates NJ 10 and NJ 15 have been identified as Pseudomonas species and Pseudomonas aeruginosa, respectively. Biodegradation studies in a soil microcosm enriched with pure cultures of the isolates demonstrated a time-dependent disappearance of 2,4-D from the 100 mg/kg herbicide-amended soil. The HPLC data analysis revealed 96.6 and 99.8% degradation in the soil inoculated with the pure cultures of isolates NJ 10 and NJ 15, respectively with in 20 days of incubation at 30 °C. Both the isolates showed significant solubilization of inorganic phosphate [Ca3(PO4)2] on the specific Pikovskaya's medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008945720327
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  • 5
    Electronic Resource
    Electronic Resource
    Compost: A study of the development process and end-product potential for suppression of turfgrass disease (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 115-134 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; biocontrol ; biological control ; compost ; disease ; fungi ; microbiology ; pathogen ; suppression ; turf ; turfgrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The relationships among the chemical, physical and biological aspects of compost and their role in suppression of turfgrass pathogens are reviewed. The composting process, mediated by microbial activity, is affected by physical and chemical characteristics which include temperature, aeration, moisture content, C:N ratio and pH. In the absence of parameter restrictions, the microbial community follows a predictable successional pattern resulting in the re-colonization of compost with metabolically active mesophilic populations that can be suppressive towards plant pathogens. Although mechanisms of suppression are not fully understood, those postulated include physiochemical and biological characteristics. The physiochemical characteristics of composts can alter suppressive properties through direct effects on pathogens and antagonistic microorganisms, or indirect effects on host systems through the supply of nutrients, improvement of soil structure, porosity and water retention capabilities, along with other factors. Biological characteristics centre on microbial community involvement in suppressive mechanisms, which can include one or a combination of competition for nutrients, antibiosis, lytic and other extracellular enzyme production, parasitism, predation and host-mediated induction of resistance. As a result of the potential benefits of compost, there is considerable interest in determining the capacity for composts to suppress turfgrass pathogens. Although the exact mechanisms of suppression are largely unknown, there appear to be several factors that play an integrated role. The use of composts that successfully suppress turfgrass diseases will permit a reduction in the use of chemical controls, and slow the development of fungicide resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008901420646
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  • 6
    Electronic Resource
    Electronic Resource
    Patulin and aflatoxin in brown rot lesion of apple fruits and their regulation (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 607-612 
    Details
    ISSN: 1573-0972
    Keywords: Aflatoxin ; apple ; fruit oils ; fungi ; patulin ; sodium hypochlorite ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Aspergillus flavus, A. niger, Penicillium expansum and Rhizopus stolonifer were the most frequently isolated fungi from healthy apple fruits. Alternaria alternata was the most common organism of rotten apple fruits, followed by A. niger, A. flavus, P. expansum and R. stolonifer. The prevalent type of decay, brown rot lesion, is caused by R. stolonifer followed by A. flavus, A. niger, A. alternata and P. expansum. Sodium hypochlorite had good curative properties against fruit rots. The main natural mycotoxins produced in rotten apple were patulin and aflatoxins. The optimum temperature for patulin production by P. expansum was 15 °C after 15 days. Complete inhibition of patulin formation was attained using 0.2% lemon oil and 〉 90% inhibition using 0.05% lemon and 0.2% orange oils. Also significant inhibition (〉 90%) of aflatoxin production was observed with 0.2% lemon oil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008982511653
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  • 7
    Electronic Resource
    Electronic Resource
    Fate of bacterial pathogens in cattle dung slurry subjected to anaerobic digestion (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 335-338 
    Details
    ISSN: 1573-0972
    Keywords: Anaerobic digestion ; biogas ; pathogens ; survival ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The survival of certain pathogenic bacteria was studied in anaerobic batch digesters at room temperature (18–25 °C) as well as at 35 °C under laboratory conditions. The survival of Escherichia coli and Salmonella typhi at room temperature was upto 20 days whereas at 35 °C it was only upto 10 days. Shigella dysenteriae was found to be the most sensitive organism which could survive upto 10 days at room temperature and upto 5 days at 35 °C. The longest survival was observed in case of Streptococcus faecalis which could survive upto 35 days at room temperature and 15 days at 35 °C. The survival time of Salmonella typhi increased when the solid contents of the digester were elevated from 9% to 15%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008906831493
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  • 8
    Electronic Resource
    Electronic Resource
    An evaluation of adaptation options for climate change impacts on agriculture in Kazakhstan (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 25-41 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; agriculture ; climate change ; Kazakhstan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Agriculture in Kazakhstan is sensitive to climate, and wheat yields could be reduced up to 70% under climate change. With the transition from a socialist economy to a free market economy, decisions are being made now that will affect Kazakhstan's ability to cope with climate change. A team of Kazakh and American researchers examined the cost-effectiveness and barriers to implementations of adaptation options for climate change. Twelve adaptation options that increase flexibility to respond to climate change were identified using a screening matrix. Four options, forecasting pest outbreaks, developing regional centers for preserving genetic diversity of seeds, supporting a transition to a free market, and reducing soil erosion through the use of changed farming practices, were examined. The Adaptation Decision Matrix (ADM) was then applied to estimate benefits using expert judgment (using an arbitrary numerical scale, not monetary values) and benefits estimates were compared to costs to determine cost-effectiveness. The ADM uses subjective measures of how well adaptation options meet policy objectives. Controlling soil erosion was estimated to have the highest benefits, but the high costs of implementation appears to make it relatively cost-ineffective. Supporting a transition to a free market was ranked as the most cost-effective measure, with regional centers second. However, use of different scales to quantify benefits or different weights can result in regional centers being more cost-effective than the transition to a free market. Regional centers was also judged to have fewer barriers to implementation than a transition to a free market. These results will be incorporated in Kazakhstan's National Action Plan. The ADM and other tools are relatively easy to apply, but are quite subjective and difficult to evaluate. The tools can be quite useful by decision makers to analyze advantages and disadvantages between different adaptation options, but should be supplemented with additional, particularly quantitative analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009626526883
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  • 9
    Electronic Resource
    Electronic Resource
    Scale, context, and decision making in agricultural adaptation to climate variability and change (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 137-165 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; agriculture ; climate change ; decision-making ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This work presents a framework for viewing agricultural adaptation, emphasizing the multiple spatial and temporal scales on which individuals and institutions process information on changes in their environment. The framework is offered as a means to gain perspective on the role of climate variability and change in agricultural adaptation, and developed for a case study of Australian agriculture. To study adaptation issues at the scale of individual farms we developed a simple modelling framework. The model highlights the decision making element of adaptation in light of uncertainty, and underscores the importance of decision information related to climate variability. Model results show that the assumption of perfect information for farmers systematically overpredicts adaptive performance. The results also suggest that farmers who make tactical planting decisions on the basis of historical climate information are outperformed by those who use even moderately successful seasonal forecast information. Analysis at continental scales highlights the prominent role of the decline in economic operating conditions on Australian agriculture. Examples from segments of the agricultural industry in Australia are given to illustrate the importance of appropriate scale attribution in adapting to environmental changes. In particular, adaptations oriented toward short time scale changes in the farming environment (droughts, market fluctuations) can be limited in their efficacy by constraints imposed by broad changes in the soil/water base and economic environment occuring over longer time scales. The case study also makes the point that adaptation must be defined in reference to some goal, which is ultimately a social and political exercise. Overall, this study highlights the importance of allowing more complexity (limited information, risk aversion, cross-scale interactions, mis-attribution of cause and effect, background context, identification of goals) in representing adaptation processes in climate change studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009636607038
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  • 10
    Electronic Resource
    Electronic Resource
    The Science of Adaptation: A Framework for Assessment (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 199-213 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; impact assessment ; response options ; vulnerability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper outlines what is meant by "adaptation" to climate change, and how it might be addressed in the IPCC Assessments. Two roles of adaptation in the climate change field are identified: adaptation as part of impact assessment (where the key question is: what adaptations are likely?), and adaptation as part of the policy response (where the central question is: what adaptations are recommended?). The concept of adaptation has been adopted in several fields including climate impact assessment and policy development, risk management, and natural hazards research. A framework for systematically defining adaptations is based on three questions: (i) adaptation to what? (ii) who or what adapts? and (iii) how does adaptation occur? The paper demonstrates that, for adaptation purposes, climate extremes and variability are integral parts of climate change, along with shifts in mean conditions. Attributes for differentiating adaptations include purposefulness, timing, temporal and spatial scope, effects, form and performance. The framework provides a guide for the treatment of adaptation in the IPCC assessments, both in the assessment of impacts and in the evaluation of adaptive policy options.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009652531101
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  • 11
    Electronic Resource
    Electronic Resource
    A Framework and Key Questions for Adapting to Climate Variability and Change (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 215-225 
    Details
    ISSN: 1573-1596
    Keywords: climate ; adaptation ; impacts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract There is a critical need to collectively understand, to develop adaptation options to enhance the benefits, and to reduce the social and economic vulnerabilities induced by climate variability and change. This paper uses key questions to help build a framework for adaptation by first organizing the questions into adaptation science, management and option components, including their respective sub-categories. The process of adaptation depends on many factors, including who or what adapts, what they adapt to, how they adapt and what and how resources are used. This conceptual model is designed to organize concepts regarding adaptation, to help stimulate ideas, and to explore the linkages among parts of the adaptation cycle. Predictive models need to be developed to determine the outcomes of planned adaptation strategies. For the best and most realistic evaluation of climate problems, adaptation and impacts should be considered together. This joint approach improves the assessment of the significance and dangers of the current and future climate, as well as the determination of solutions (e.g., how to prepare for a changing climate) and their priorities. Challenges of adaptive management are discussed in terms of a framework with linkages to adaptation science and options. Adaptation research and applications work continue to build on the foundation of science and management frameworks to address the risks and uncertainties in the decision-making process and to identify adaptation options.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009660700150
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  • 12
    Electronic Resource
    Electronic Resource
    Data Requirements for Developing Adaptations to Climate Variability and Change (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 227-237 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; climate variability ; data ; climate applications ; El Niño ; UNFCCC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract An extensive foundation of high quality data and information on the climate and on the biological, environmental and social systems affected by climate is required in order to understand the climate impact processes involved, to develop new adaptation practices, and to subsequently implement these practices. Experience of the impacts of current and past variability of climate and sea level is a prime source of information. Many practices are in use to reduce climate impacts, for example in engineering design, agricultural risk management and climate prediction services, though their roles as adaptations to climate change are not widely appreciated. While there are good data sets on some factors and in some regions, in many cases the databases are inadequate and there are few data sets on adaptation-specific quantities such as vulnerability, resilience and adaptation effectiveness. Current international action under the United Nations Framework Convention on Climate Change (UNFCCC) pays little attention to adaptation and its information requirements. Furthermore there are trends toward reduced data gathering and to restrictions on access to data sets, especially arising from cost and commercialisation pressures. To effectively respond to the changes in climate that are now inevitable, governments will need to more clearly identify adaptation as a central feature of climate change policy and make a renewed shared commitment to collecting and freely exchanging the necessary data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009668902876
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  • 13
    Electronic Resource
    Electronic Resource
    Coastal Adaptation to Climate Change: Can the IPCC Technical Guidelines be applied? (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 239-252 
    Details
    ISSN: 1573-1596
    Keywords: climate change ; coastal zones ; adaptation ; vulnerability ; IPCC Technical Guidelines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper evaluates the IPCC Technical Guidelines for Assessing Climate Change Impacts and Adaptations with respect to the guidance offered for coastal-adaptation assessment. It appears that the IPCC Technical Guidelines focus strongly on implementation. This paper uses both conceptual and empirical information is used in this paper to show that coastal adaptation embraces more than selecting one of the "technical" options to respond to sea-level rise (retreat, accommodate or protect). Coastal adaptation is a more complex and iterative process with a series of policy cycles. To be effective, an expanded adapta-tion framework involving four steps is suggested, including (i) information collection and awareness raising; (ii) planning and design; (iii) implementation; and (iv) monitoring and evaluation. The incom-plete coverage of these four steps in existing coastal-adaptation assessments constrains the development of adaptation strategies that are supported by the relevant actors and integrated into existing management. Researchers and policy-makers are recommended to work together to establish a framework for adaptation that is integrated within current coastal management processes and practices and takes a broader view on the subject.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009681207419
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  • 14
    Electronic Resource
    Electronic Resource
    Risk and Uncertainties, Analysis and Evaluation: Lessons for Adaptation and Integration (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 319-329 
    Details
    ISSN: 1573-1596
    Keywords: uncertainty ; risk ; adaptation ; extreme events ; (credible) information ; integrated assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This paper draws ten lessons from analyses of adaptation to climate change under conditions of risk and uncertainty: (1) Socio-economic systems will likely respond most to extreme realizations of climate change. (2) Systems have been responding to variations in climate for centuries. (3) Future change will effect future citizens and their institutions. (4) Human systems can be the sources of surprise. (5) Perceptions of risk depend upon welfare valuations that depend upon expectations. (6) Adaptive decisions will be made in response to climate change and climate change policy. (7) Analysis of adaptive decisions should recognize the second-best context of those decisions. (8) Climate change offers opportunity as well as risk. (9) All plausible futures should be explored. (10) Multiple methodological approaches should be accommodated. These lessons support two pieces of advice for the Third Assessment Report: (1) Work toward consensus, but not at the expense of thorough examination and reporting of the "tails" of the distributions of the future. (2) Integrated assessment is only one unifying methodology; others that can better accommodate those tails should be encouraged and embraced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009619806936
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  • 15
    Electronic Resource
    Electronic Resource
    A Framework for Benefit-Cost Analysis of Adaptation to Climate Change and Climate Variability (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 307-318 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; climatic change ; economic welfare ; costs and benefits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential damages of climate change and climate variability are dependent upon the responses or adaptations that people make to their changing environment. By adapting the management of resources, the mix and methods of producing goods and services, choices of leisure activities, and other behavior, people can lessen the damages that would otherwise result. A framework for assessing the benefits and costs of adaptation to both climate change and climate variability is described in the paper. The framework is also suitable for evaluating the economic welfare effects of climate change, allowing for autonomous adaptation by private agents. The paper also briefly addresses complications introduced by uncertainty regarding the benefits of adaptation and irreversibility of investments in adaptation. When investment costs are irreversible and there is uncertainty about benefits, the usual net present value criterion for evaluating the investment gives the wrong decision. If delaying an adaptation project is possible, and if delay will permit learning about future benefits of adaptation, it may be preferable to delay the project even if the expected net present value is positive. Implications of this result for adaptation policy are discussed in the paper.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009667706027
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  • 16
    Electronic Resource
    Electronic Resource
    Adaptation to the Impacts of Sea Level Rise in Egypt (1999)
    Springer
    Mitigation and adaptation strategies for global change 4 (1999), S. 343-361 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; climate change ; socioeconomic impacts ; Egypt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Assessment of the vulnerability and expected socioeconomic losses over the Nile delta coast due to the impact of sea level rise is carried out in details. Impacts of sea level rise over the Governorates of Alexandria and Port Said in particular, are evaluated quantitatively. Analysis of the results at Alexandria Governorate indicate that, if no action is taken, an area of about 30% of the city will be lost due to inundation. Almost 2 million people will have to abandon their homeland; 195,000 jobs will be lost and an economic loss of over $3.5 Billion is expected over the next century. At Port Said Governorate results indicate that beach areas are most severely affected (hence tourism), followed by urban areas. The agriculture sector is the least affected sector. It is estimated that the economic loss is over $ 2.0 Billion for 0.50 m SLR and may exceed $ 4.4 Billion for 1.25 m SLR. Options and costs of adaptation are analyzed and presented. Multi-criteria and decision matrix approaches, based on questionnaire surveys are carried out to identify priorities for the two cases. Analysis of these techniques of two options; the current policy (hard protection measures on some vulnerable areas) and no action (stopping these activities) have the lowest scores. Beach nourishment and integrated coastal zone management (ICZM) have the highest scores, however ICZM has high cost measures. The most cost-effective option is the land-use change, however with relatively very high cost measure. It is recommended that an ICZM approach be adopted since it provides a reasonable trade off between costs and cost effectiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009684210570
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  • 17
    Electronic Resource
    Electronic Resource
    Enhanced monoclonal antibody production by gradual increase of osmotic pressure (1999)
    Springer
    Cytotechnology 29 (1999), S. 27-33 
    Details
    ISSN: 1573-0778
    Keywords: adaptation ; antibody production rate ; hybridoma ; intracellular amino acids ; osmotic pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The time length required for the adaptation of AFP-27 hybridoma cells to high osmotic pressure and the effect of a gradual increase of osmotic pressure on monoclonal antibody production were investigated. When the cells were subjected to an increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg- 1, the intracellular content of osmoprotective free amino acids reached a maximum level 6 h after the osmotic pressure was increased to 366 mOsmol kg-1. The same time period of 6 h incubation at 366 mOsmol kg-1 was required to obtain a high growth rate of AFP-27 cells at 440 mOsmol kg-1 when the cells were subjected to a two-step increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg-1 and then to 440 mOsmol kg-1. The time length for the physiological adaptation of the cells to 366 mOsmol kg-1 was consequently estimated to be 6 h. Osmotic pressure during batch cultivation was gradually increased from 300 mOsmol kg-1 to 400 mOsmol kg-1 with an adaptation time of at least 6 h. The specific growth rates following a gradual increase of osmotic pressure were higher than those at a constant osmotic pressure of 400 mOsmol kg-1, while the specific monoclonal antibody production rate increased with the increase in the mean osmotic pressure. As a result, the cells grown under a gradual increase of osmotic pressure produced higher amounts of monoclonal antibodies than did those grown under constant osmotic pressure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008016806599
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  • 18
    Electronic Resource
    Electronic Resource
    Recognition and molecular evolution in bacteria (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 7-10 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; evolution ; molecular ; pairing ; recognition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract One principal function of biological molecules in bacteria is to recognize other molecules. This allows cells to assemble for regulated enzymatic catalysis and the integration of biochemical pathways. Recognition is also an essential and a specific property in base pairing of DNA in the double helix. Therefore, recognition events must have been central to early self-assembly of primitive genetic material, genomes, cells, genetic recombination and especially in enzyme-substrate-product recognition events. Molecular recognition events are examined with an emphasis on their central role in early prokaryotic evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008882931061
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  • 19
    Electronic Resource
    Electronic Resource
    Why on Earth: Self-assembly of the first bacterial cell to abundantand diverse bacterial species (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 297-304 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; cell ; diversity ; division ; Earth ; evolution ; metabolism ; self-assembly ; surfaces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract About 80% of the evolutionary history of life on Earth is restricted to microorganisms which have had several billion years to speciate. The reasons for the origin (self-assembly) of life on Earth, bacterial cell division and why there are so many different bacteria and their global dispersal are discussed from an evolutionary perspective.
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    URL: http://dx.doi.org/10.1023/A:1008962900542
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    Evolution of gene transfer in bacteria (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 1-6 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; conjugation ; DNA ; evolution ; gene transfer ; transduction ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The transfer of genetic information by transformation, conjugation and transduction in bacteria occurs frequently in nature. These diverse gene transfer mechanisms in bacteria are the result of evolution and are not linked to reproduction as in eukaryotic organisms. In this review, gene transfer in bacteria will be considered from an evolutionary perspective.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008830914223
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  • 21
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    Effect of temperatures during pure culture fermentation of Kinema (1998)
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 847-850 
    Details
    ISSN: 1573-0972
    Keywords: Kinema ; soybean ; Bacillus subtilis KK2:B10 ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Kinema was prepared by fermenting whole cooked soybeans with pure culture of Bacillus subtilis KK2:B10 (MTCC 2756) strain at 35°C, 40°C and 45°C for 24h. Temperature, mesophilic plate counts, relative viscosity, water-soluble nitrogen, formal nitrogen contents and reducing sugars of fermenting soybeans were investigated during fermentation. At higher temperatures the growth rate of B. subtilis KK2:B10 was faster. A remarkable increase in the relative viscosity of kinema was observed at 40°C during fermentation. Water-soluble nitrogen and formol nitrogen to total nitrogen contents increased throughout the 24h of fermentation. Reducing sugars increased during the log phase and then decreased sharply. Kinema matured below 10°C for 1 day after the desired fermentation showed a significant increase in relative viscosity. The quality of kinema was maintained with pure culture fermentation by B. subtilis KK2:B10 at 40°C for 20h and matured at 5°C for 1 day.
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    URL: http://dx.doi.org/10.1023/A:1008867511369
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    Russian human geography in the 'wonderland' of globalization (1998)
    Springer
    GeoJournal 45 (1998), S. 77-83 
    Details
    ISSN: 1572-9893
    Keywords: globalization ; national tradition ; Russian human geography inertia ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006845428890
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    Enhancement of bacterial efficiency for metal removal using mutation techniques (1998)
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 853-856 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; heavy metals ; mutation ; enhancement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The objective of the present study was to obtain by mutation and selection techniques bacterial strains capable of removing heavy metals at high efficiency. Four of the bacteria most promising in metal uptake, Staphylococcus aureus, Bacillus Sphaericus, B. licheniformis and Arthrobacter sp. were selected after isolation from water heavily polluted with heavy metals. Two mutagenic agents were used: U.V. irradiation at 245nm (physical) and 1% ethidium bromide (chemical). Optimum conditions for metal removal by most of the tested bacteria were: pH 9, 50°C and 200rev/min agitation speed. Induction of mutation both physically or chemically resulted in mutants that were superior over their wild types in removing heavy metals under investigation. The highest removal efficiencies (REs) achieved were in the following order: Cd(89.9–100%); Cr(87.3–99.7%); Zn(47.7–100%); Cu(40.8–84.7%); Pb(40.2–51%); Fe(17.5–28.7%); Ni(13.8–23.9%) and finally Co(17.2–18.4%). Using mixed cultures of the wild and the selected mutants enhanced the RE(s) of some metals compared to those obtained by individual species, and the time required to achieve the highest RE was reduced.
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    URL: http://dx.doi.org/10.1023/A:1008827830024
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    Electronic Resource
    Electronic Resource
    Adapting to Climate Change in Africa (1997)
    Springer
    Mitigation and adaptation strategies for global change 2 (1997), S. 19-44 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; Africa ; agriculture ; climate change ; vulnerability ; water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The intersection of present vulnerability and the prospect of climate change in Africa warrants proactive action now to reduce the risk of large-scale, adverse impacts. The process of planning adaptive strategies requires a systematic evaluation of priorities and constraints, and the involvement of stakeholders. An overview of climate change in Africa and case studies of impacts for agriculture and water underlie discussion of a typology of adaptive responses that may be most effective for different stakeholders. The most effective strategies are likely to be to reduce present vulnerability and to enhance a broad spectrum of capacity in responding to environmental, resource and economic perturbations. In some cases, such as design of water systems, an added risk factor should be considered.
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    URL: http://dx.doi.org/10.1023/B:MITI.0000004663.31074.64
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  • 25
    Electronic Resource
    Electronic Resource
    Adapting to climate change in Africa (1997)
    Springer
    Mitigation and adaptation strategies for global change 2 (1997), S. 19-44 
    Details
    ISSN: 1573-1596
    Keywords: adaptation ; Africa ; agriculture ; climate change ; vulnerability ; water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The intersection of present vulnerability and the prospect of climate change in Africa warrants proactive action now to reduce the risk of large-scale, adverse impacts. The process of planning adaptive strategies requires a systematic evaluation of priorities and constraints, and the involvement of stakeholders. An overview of climate change in Africa and case studies of impacts for agriculture and water underlie discussion of a typology of adaptive responses that may be most effective for different stakeholders. The most effective strategies are likely to be to reduce present vulnerability and to enhance a broad spectrum of capacity in responding to environmental, resource and economic perturbations. In some cases, such as design of water systems, an added risk factor should be considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02437055
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  • 26
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    Impact of Climate Change on Maize Yield in Central and Northern Greece: A Simulation Study with Ceres-Maize (1997)
    Springer
    Mitigation and adaptation strategies for global change 1 (1997), S. 251-271 
    Details
    ISSN: 1573-1596
    Keywords: Greece ; maize ; climate change ; CO2 effects ; adaptation ; crop simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential impacts of climate change on the phenology and yield of two maize varieties in Greece were studied. Three sites representing the central and northern agricultural regions were selected: Karditsa, Naoussa and Xanthi. The CERES-Maize model, embedded in the Decision Support System for Agrotechnology Transfer (DSSAT 3.0), was used for the crop simulations, with current and possible future management practices. Equilibrium doubled CO2 climate change scenarios were derived from the GISS, GFDL, and UKMO general circulation models (GCMs); a transient scenario was developed from the GISS GCM transient run A. These scenarios predict consistent increases in air temperature, small increases in solar radiation and precipitation changes that vary considerably over the study regions in Greece. Physiological effects of CO2 on crop growth and yield were simulated. Under present management practices, the climate change scenarios generally resulted in decreases in maize yield due to reduced duration of the growing period at all sites. Adaptation analyses showed that mitigation of climate change effects may be achieved through earlier sowing dates and the use of new maize varieties. Varieties with higher kernel-filling rates, currently restricted to the central regions, could be extended to the northern regions of Greece. In the central regions, new maize varieties with longer grain-filling periods might be needed.
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    URL: http://dx.doi.org/10.1023/B:MITI.0000018044.48957.28
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  • 27
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    Modelling Crop-Climate Interactions in Spain: Vulnerability and Adaptation of Different Agricultural Systems to Climate Change (1997)
    Springer
    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    Details
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
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    URL: http://dx.doi.org/10.1023/B:MITI.0000018139.42373.f1
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  • 28
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    Impact of Climate Change on Possible Scenarios for Egyptian Agriculture in the Future (1997)
    Springer
    Mitigation and adaptation strategies for global change 1 (1997), S. 233-250 
    Details
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Egypt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract If no timely measures are taken to adapt Egyptian agriculture to possible climate warming, the effects may be negative and serious. Egypt appears to be particularly vulnerable to climate change because of its dependence on the Nile River as the primary water source, its large traditional agricultural base, and its long coastline, already undergoing both intensifying development and erosion. A simulation study characterized potential yield and water use efficiency decreases on two reference crops in the main agricultural regions with possible future climatic variation, even when the beneficial effects of increased CO2 were taken into account. On-farm adaptation techniques which imply no additional cost to the agricultural system, did not compensate for the yield losses with the warmer climate or improve the crop water-use efficiency. Economic adjustments such as the improvement of the overall water-use efficiency of the agricultural system, soil drainage and conservation, land management, and crop alternatives are essential. If appropriate measures are taken, negative effects of climate change in agricultural production and other major resource sectors (water and land) may be lessened.
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    URL: http://dx.doi.org/10.1023/B:MITI.0000018270.16497.39
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  • 29
    Electronic Resource
    Electronic Resource
    Modelling crop-climate interactions in Spain: Vulnerability and adaptation of different agricultural systems to climate change (1997)
    Springer
    Mitigation and adaptation strategies for global change 1 (1997), S. 273-288 
    Details
    ISSN: 1573-1596
    Keywords: climate change ; vulnerability ; adaptation ; agriculture ; Spain ; wheat ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract This study evaluates the theoretical impact of climate change on yields and water use of two crops with different responses to increased CO2 and which represent contrasting agricultural systems in Spain. In all cases the simulated effects of a CO2-induced climate change depended on the counteracting effects between higher daily ET rates, shortening of crop growth duration and changes in precipitation patterns as well as the simulated effects of CO2 on the water use efficiency of the crops. For summer irrigated crops such as maize, the yield reductions and the exacerbated problems of irrigation water availability simulated with climate change may force the crop out of production in some regions. For winter dryland crops such as wheat, productivity increased significantly in some regions, suggesting a northward shift of area suitable for wheat production in future climates. The study considered strategies for improving the efficiency of water use based on the optimization of crop management decisions in a CO2-driven warmer climate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00517807
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    Impact of climate change on maize yield in central and northern Greece: A simulation study with CERES-Maize (1997)
    Springer
    Mitigation and adaptation strategies for global change 1 (1997), S. 251-271 
    Details
    ISSN: 1573-1596
    Keywords: Greece ; maize ; climate change ; CO2 effects ; adaptation ; crop simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract The potential impacts of climate change on the phenology and yield of two maize varieties in Greece were studied. Three sites representing the central and northern agricultural regions were selected: Karditsa, Naoussa and Xanthi. The CERES-Maize model, embedded in the Decision Support System for Agrotechnology Transfer (DSSAT 3.0), was used for the crop simulations, with current and possible future management practices. Equilibrium doubled CO2 climate change scenarios were derived from the GISS, GFDL, and UKMO general circulation models (GCMs); a transient scenario was developed from the GISS GCM transient run A. These scenarios predict consistent increases in air temperature, small increases in solar radiation and precipitation changes that vary considerably over the study regions in Greece. Physiological effects of CO2 on crop growth and yield were simulated. Under present management practices, the climate change scenarios generally resulted in decreases in maize yield due to reduced duration of the growing period at all sites. Adaptation analyses showed that mitigation of climate change effects may be achieved through earlier sowing dates and the use of new maize varieties. Varieties with higher kernel-filling rates, currently restricted to the central regions, could be extended to the northern regions of Greece. In the central regions, new maize varieties with longer grain-filling periods might be needed.
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    URL: http://dx.doi.org/10.1007/BF00517806
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    On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells (1997)
    Springer
    Cytotechnology 24 (1997), S. 19-30 
    Details
    ISSN: 1573-0778
    Keywords: fluidized-bed reactor ; monoclonal antibody ; on-line monitoring ; sample system ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The monoclonal-antibody production of an immobilized hybridoma cell line cultivated in a fluidized-bed reactor was monitored on-line for nearly 900 h. The monoclonal antibody concentration was determined by an immuno affinity-chromatography method (ABICAP). Antibodies directed against the product, e.g. IgG, were immobilized on a micro-porous gel and packed in small columns. After all IgG present in the sample was bound to the immobilized antibodies, unbound proteins were removed by rinsing the column. Elution of the bound antibodies followed and the antibodies were determined by fluorescence. The analytical procedure was automated with a robotic device to enable on-line measurements. The correlation between the on-line determined data and antibody concentrations measured by HPLC was linear. A sampling system was constructed, which was based on a pneumatically actuated in-line membrane valve integrated into the circulation loop of the reactor. Separation of the cells from the sample stream was achieved by a depth filter made of glass-fibre, situated outside the reactor. Rapid obstruction of the filter by cells or cell debris and contamination of the sample system was avoided by intermittent rinsing of the sample system with a chemical solution. The intermittent rinsing of the filter, which had a surface of 4.8 cm2, resulted in an operational capacity of up to 40 samples (1.0 l total sample volume). Both the sampling system and the analytical device functioned without failure during this long-term culture. The culture temperature was varied between 34 and 40 °C. Raising the temperature from 34 up to 37 °C resulted in a simultaneous increase of growth and specific antibody production rate. Specific metabolic rates of glucose, lactate, glutamine and ammonium stayed constant in this temperature range. A further enhancement of temperature up to 40 °C had a negative effect on the growth rate, whereas the specific monoclonal antibody production rate showed a small increase. The other specific metabolic rates also increased in the temperature range between 38 to 40 °C.
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    URL: http://dx.doi.org/10.1023/A:1007913128209
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    The theory of everything and molecular evolution (1997)
    Springer
    World journal of microbiology and biotechnology 14 (1997), S. 171-176 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; DNA ; evolution ; molecular ; optimization ; RNA ; theory ; time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The theory of everything is discussed in relationship to early bacterial molecular evolution. The emphasis is on time, space (or location at the molecular level), the universal construction kit (elements contained in periodic table) and change per units of time that were necessary for early bacterial molecular evolution to occur.
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    URL: http://dx.doi.org/10.1023/A:1008817726536
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    The effect of fumonisin B1 on the growth of bacteria (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 539-543 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; fumonisins ; metabolism ; mycotoxins ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Fumonisins are mycotoxins produced by several Fusarium species which are common contaminants of maize and maize products. Their toxicity and carcinogenicity for animals ingesting these toxins have been clearly demonstrated, but, there are no studies on the effect of fumonisins on bacteria. In this study various Gram-positive and Gram-negative bacteria including typical representatives of the human intestinal flora were grown in the presence of 50 to 1000 μM fumonisin B1 and the fumonisin concentrations in the culture medium were determined after incubation for a certain period. No inhibition of bacterial growth was observed, indicating that this mycotoxin is non-toxic for the bacteria investigated. There was no indication that fumonisin B1 was metabolized by the bacteria as its concentration in the culture medium did not decrease during the incubation period.
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    URL: http://dx.doi.org/10.1023/A:1018513308847
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    Isolation of polyhydroxyalkanoate-producing bacteria from an integrated-farming pond and palm-oil mill effluent ponds (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 707-709 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; integrated-farming pond ; isolation ; palm-oil mill effluent ; PHA ; screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Bacterial isolates from two environments, an integrated-farming pond in the university and palm-oil mill effluent (POME) ponds at a local palm-oil-processing factory, were screened for polyhydroxyalkanoates (PHAs). Initially Sudan Black B staining was performed to detect lipid cellular inclusions. Lipid-positive isolates were then grown in a nitrogen-limiting medium containing 2% (w/v) glucose to promote accumulation of PHA before the subsequent Nile Blue A staining. The PHA extracted from positive isolates was confirmed by nuclear magnetic resonance (NMR) spectroscopy. The proportion of PHA-positive bacterial isolates was higher in the POME ponds compared to the integrated-farming pond.
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    URL: http://dx.doi.org/10.1023/A:1018535308364
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    Bacterial population genetics (1997)
    Springer
    World journal of microbiology and biotechnology 14 (1997), S. 1-5 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; DNA ; evolution ; genetics ; genome ; molecular ; population
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Bacterial population genetics is the study of natural bacterial genetic diversity arising from evolutionary processes. The roles of molecular mistakes, restriction–modification, plasmids and gene transfer in bacteria are also important components of population genetics. These aspects are of considerable scientific importance from a fundamental perspective, because of the short generation times of bacteria, their microscopic cell size, the large population sizes bacteria can achieve and their different mechanisms of gene transfer.
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    URL: http://dx.doi.org/10.1023/A:1008897128837
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    Short communication: Isolation of a bacterium capable of limited degradation of industrial and labelled, natural and synthetic lignins (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 111-112 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; degradation ; Kraft-lignin ; lignocellulose ; synthetic lignin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Pseudomonas putida, isolated from decomposing plant materials, degraded several lignin-related aromatic compounds. After 30 days of incubation in media containing polymeric Kraft-lignin (PKL), the amount of Klason lignin had decreased by about 13%. When 14C-labelled dehydropolymers of coniferyl alcohol (DHP) lignins and 14C-lignin-lignocelluloses were used as substrates, mineralization to 14CO2 by the P. putida strain ranged from 1.4% to 2.1%.
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    URL: http://dx.doi.org/10.1007/BF00327817
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    Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production (1996)
    Springer
    Cytotechnology 21 (1996), S. 95-109 
    Details
    ISSN: 1573-0778
    Keywords: adaptation ; hybridoma ; monoclonal antibody ; protein free medium ; suspension culture ; weaning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, and ethanolamine. No protein or lipid components were added. All three cell lines were weaned off serum using CDSS and a commercially available protein free medium PFHM-II. Data shown here indicated that normally cells took 1–7 weeks to wean off serum and an additional 2–7 weeks to adapt to suspension culture. After adaptation the cells were able to grow well in suspension culture using both protein free media and in the main performed better than serum containing controls. The stability of the three hybridoma cells for antibody production following freeze/thaw procedures and long term subculturing was also tested. All three lines were frozen using our protein free CDSS medium (containing 0.75% bovine serum albumin and 10% dimethyl sulfoxide) in liquid nitrogen for up to one year. Cells thawed from these stocks recovered well and were able to maintain good growth and antibody production characteristics. One line was shown to grow using our protein free CDSS medium in suspension culture for 12 weeks without loss of antibody productivity.
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    URL: http://dx.doi.org/10.1007/BF02215660
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    Factors determining rock phosphate solubilization by microorganisms isolated from soil (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 567-572 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; fungi ; rock phosphate ; solubilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Forty two soil isolates (31 bacteria and 11 fungi) were studied for their ability to solubilize rock phosphate and calcium phosphate in culture medium. Eight bacteria and 8 fungi possessed solubilizing ability. Pseudomonas cepacia and Penicillium purpurogenum showed the highest activity. There was a correlation between final pH value and titratable acidity (r=−0.29 to −0.87) and between titratable acidity and soluble phosphate (r=0.22 to 0.99). Correlation values were functions of insoluble phosphate and of the group of microorganisms considered. A high correlation was observed between final pH and soluble phosphate only for the rock phosphates inoculated with the highest concentration of solubilizing bacteria (r=−0.73 to −0.98).
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    URL: http://dx.doi.org/10.1007/BF00327716
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    Use of agricultural surpluses for production of biomass by marine microalgae (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 47-49 
    Details
    ISSN: 1573-0972
    Keywords: Bacteria ; fermentation ; flour ; microalgal biomass ; potato ; rye ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The marine microalga Phaeodactylum tricornutum was cultivated in semi-continuous culture under mixotrophic conditions with the soluble fractions of potato, rye and wheat flours that had been naturally fermented, at 2% or 4% (w/v). The rye flour produced the highest microalgal cellular density of 90×106 cells.ml-1 when supplemented with NaNO3 and NaH2PO4. The autotrophic control only gave 57×106 cells.ml-1. The value of agricultural surpluses, such as rye flour, can therefore be increased by its use in the production of valuable, microalgal biomass which is rich in protein, pigments and fatty acids.
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    URL: http://dx.doi.org/10.1007/BF00327799
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    A rapid screening method for the isolation of metal-accumulating microorganisms (1995)
    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 213-217 
    Details
    ISSN: 1476-5535
    Keywords: Screening ; Method ; Heavy metal ; Bioaccumulation ; Biosorption ; Microorganisms ; Bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary An agar plate screening method was developed for the rapid isolation of heavy metal-accumulating microorganisms and preliminary estimation of their biosorption capacity. The test is based on the visulaization and interpretation of the metal distribution between agar and colonies by chemical preciptitation with hydrogen sulphide or ammonium sulphide. The heavy metals silver, thallium, lead, copper, nickel and cadmium have been tested successfully. The efficiency of the method is demonstrated for isolating silver-accumulating bacterian and estimating silver biosorption capacity.
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    URL: http://dx.doi.org/10.1007/BF01569930
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    Metabolism of hyperthermophiles (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 26-57 
    Details
    ISSN: 1573-0972
    Keywords: Acetate formation ; acetyl-CoA oxidation ; Archaea ; Bacteria ; chemolithoautotroph ; chemoorganoheterotroph ; glycolytic pathway ; hyperthermophiles ; metabolic pathways ; peptide metabolism ; sugar metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Hyperthermophiles are characterized by a temperature optimum for growth between 80 and 110°C. They are considered to represent the most ancient phenotype of living organisms and thus their metabolic design might reflect the situation at an early stage of evolution. Their modes of metabolism are diverse and include chemolithoautotrophic and chemoorganoheterotrophic. No extant phototrophic hyperthermophiles are known. Lithotrophic energy metabolism is mostly anaerobic or microaerophilic and based on the oxidation of H2 or S coupled to the reduction of S, SO inf4 sup2- , CO2 and NO inf3 sup- but rarely to O2. the substrates are derived from volcanic activities in hyperthermophilic habitats. The lithotrophic energy metabolism of hyperthermophiles appears to be similar to that of mesophiles. Autotrophic CO2 fixation proceeds via the reductive citric acid cycle, considered to be one of the first metabolic cycles, and via the reductive acetyl-CoA/carbon monoxide dehydrogenase pathway. The Calvin cycle has not been found in hyperthermophiles (or any Archaea). Organotrophic metabolism mainly involves peptides and sugars as substrates, which are either oxidized to CO2 by external electron acceptors or fermented to acetate and other products. Sugar catabolism in hyperthermophiles involves non-phosphorylated versions of the Entner-Doudoroff pathway and modified versions of the Embden-Meyerhof pathway. The ‘classical’ Embden-Meyerhof pathway is present in hyperthermophilic Bacteria (Thermotoga) but not in Archaea. All hyperthermophiles (and Archaea) tested so far utilize pyruvate:ferredoxin oxidoreductase for acetyl-CoA formation from pyruvate. Acetyl-CoA oxidation in anaerobic sulphur-reducing and aerobic hyperthermophiles proceeds via the citric acid cycle; in the hyperthermophilic sulphate-reducer Archaeoglobus an oxidative acetyl-CoA/carbon monoxide dehydrogenase pathway is operative. Acetate formation from acetyl-CoA in Archaea, including hyperthermophiles, is catalysed by acetyl-CoA synthetase (ADP-forming), a novel prokarvotic enzyme involved in energy conservation. In Bacteria, including the hyperthermophile Thermotoga, acetyl-CoA conversion to acetate involves two enzymes, phosphate acetyltransferase and acetate kinase.
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    Evaluation of climatic change through harmonic analysis (1994)
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    Natural hazards 9 (1994), S. 5-16 
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    ISSN: 1573-0840
    Keywords: Fourier transform ; maximum entropy spectral analysis ; precipitation ; temperature ; climatic change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract In the present work, a precipitation and temperature series from Barcelona (Spain) are analysed in order to detect the possible existence of climatic changes or cycles. The analysis is carried out both from the temporal and spectral standpoints. The techniques used range from the classical periodogram and Blackman-Tukey method through to the Maximum Entropy method. The results do not show the existence of climatic cycles, though they do show a clear tendency toward increased precipitation and decreased temperature, since the last years of series.
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    Bacterial species in raw and cured compost from a large-scale urban composter (1994)
    Springer
    Journal of industrial microbiology and biotechnology 13 (1994), S. 177-182 
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    ISSN: 1476-5535
    Keywords: Antibiotic resistance ; Bacteria ; Compost ; Metal-resistance ; Thermophilic bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Raw and cured compost samples from a large-scale urban composter were studied over a period of eight months to gain information on bacterial species present. Total viable, aerobic heterotrophic bacteria, lactose-positive bacteria, antibiotic and metal-resistant bacteria and thermophilic bacteria were enumerated. Both raw and cured compost samples contained metal and antibiotic-tolerant bacteria (〈Log 3.0 to Log 8.5 CFU g−1 compost) as well as high numbers (as high as Log 7.4 CFU g−1 dry weight compost) of thermophilic bacteria isolated by growth at 55 °C. Selected colonies were also identified using the Biolog 95 substrate identification system.Escherichia coli andSalmonella spp. were not detected in compost samples.
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    Low temperature cultivation — A step towards process optimisation (1994)
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    Cytotechnology 15 (1994), S. 111-116 
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    ISSN: 1573-0778
    Keywords: Adherent animal cells ; glucose ; lactate ; productivity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37°C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.
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    Microbial contaminants of commercially bottled non-alcoholic drinks produced in Nigeria (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 488-490 
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    ISSN: 1573-0972
    Keywords: Bacteria ; microbial load ; moulds ; non-alcoholic drinks ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract When microbiological analyses were conducted on 90 samples of soft drinks representing 30 different products commercially available in Nigeria, contaminants were detected in 50% of them. The isolates were mainly saprophytic and non-pathogenic: Bacillus spp. (35%), Lactobacillus spp. (26%), Pediococcus spp. (6%), Staphylococcus epidermidis (6%) and Micrococcus spp. (3%) accounted for the bacterial isolates while Aspergillus niger (6%) and Saccharomyces spp. (16%) accounted for the fungal isolates.
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    Bacteriological conditions of a Jamaican chicken hatchery (1993)
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    World journal of microbiology and biotechnology 9 (1993), S. 282-286 
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    ISSN: 1573-0972
    Keywords: Bacteria ; chicken ; hatchery ; microbiological assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The bacteriological profile of a chicken hatchery in Jamaica was examined. The bacterial numbers in each room of the hatchery and the effect of washing with disinfectant on the bacterial population were determined. A representative number of the bacterial isolates before and after washing the hatchery was identified. The results indicate that, while washing with quaternary ammonium compounds did not affect the Gram-negative bacteria, the number of Gram-positive bacteria was significantly decreased. Bacteria of the genera Pseudomonas, Plesiomonas and Enterobacter were predominant in the post-washing flora. The water used to wash the hatchery was contaminated and therefore a possible source of contamination.
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    Evaluation of different alkali treatments of bagasse pith for cultivation of Cellulomonas sp. (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 213-215 
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    ISSN: 1573-0972
    Keywords: Bacteria ; biomass ; lignocellulosic ; pre-treatment ; single cell protein ; sodium hydroxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Biomass production of Cellulomonas was optimal with 1% (w/v) bagasse pith pre-treated with either 0.2 M NaOH for 1 h at 80°C or 0.4 M NaOH for 40 h at 28 to 30°C. Growth was similar to that obtained with a more severe treatment used as control and compared well with other reports for cellulolytic bacteria cultivated on pre-treated bagasse pith.
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    Control of the expression of bacterial genes involved in symbiotic nitrogen fixation (1993)
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    World journal of microbiology and biotechnology 9 (1993), S. 444-454 
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    ISSN: 1573-0972
    Keywords: Bacteria ; expression ; genes ; nitrogen fixation ; symbiotic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Several genera of N2-fixing bacteria establish symbiotic associations with plants. Among these, the genus Rhizobium has the most significant contribution, in terms of yield, in many important crop plants. The establishment of the Rhizobium-legume symbiosis is a very complex process involving many genes which need to be co-ordinately regulated. In the first instance, plant signal molecules, known to be flavonoids, trigger the expression of host-specific genes in the bacterial partner through the action of the regulatory NodD protein. In response to these signals, Rhizobium bacteria synthesize lipo-oligosaccharide molecules which in turn cause cell differentiation and nodule development. Once the nodule has formed, Rhizobium cells differentiate into bacteroids and another set of genes is activated. These genes, designated nif and fix, are responsible for N2 fixation. In this system, several regulatory proteins are involved in a complex manner, the most important being NifA and a two component (FixK and FixL) regulatory system. Our knowledge about the establishment of these symbioses has advanced recently, although there are many questions yet to be solved.
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    Detection of Vibrio cholerae O1 in the aquatic environment in Brazil employing direct immunofluorescence microscopy (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 390-392 
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    ISSN: 1573-0972
    Keywords: Bacteria ; cholera ; environment ; immunofluorescence ; Vibrio ; water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Culturing and immunofluorescence (FA) methods for detection of Vibrio cholerae O1 in samples collected from the aquatic environment at selected sites in Brazil were compared. Of the samples examined, 90% were positive for V. cholerae O1 by FA but none was positive by culture, although strains of V. cholerae other than O1 strains were readily isolated. Evidence for V. cholerae O1 being autochthonous to the aquatic environment of Brazil is presented. Furthermore, FA methods are recommended for cholera surveillance programmes directed at the natural environment.
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    A bacteriological profile of bottled water sold in Bangladesh (1992)
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    World journal of microbiology and biotechnology 8 (1992), S. 544-545 
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    ISSN: 1573-0972
    Keywords: Bacteria ; Bangladesh ; bottled water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Four different brands of bottled water had counts of aerobic bacteria ranging from 103 to 106 colony forming units/100 ml. Pathogens isolated includedPseudomonas (in all brands),Alcaligenes andEscherichia coli (each in three brands), andKlebsiella, Enterobacter andShigella (each in two brands). All four brands were judged to be unsatisfactory by accepted health standards.
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    Cadmium sorption by bacteria and freshwater sediment (1991)
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 201-207 
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    ISSN: 1476-5535
    Keywords: Diffusion chamber ; Cadmium-sensitive ; Cadmium-resitant ; Sediment ; Bacteria ; Cadmium-sorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Sorption of cadmium by sediment bacteria and freshwater sediment was investigated using diffusion chambers to simulate the water-sediment interface. Diffusion chambers were constructed to provide two compartments separated by a dialysis membrane. Diffusion of cadmium across the membrane was monitored after pure cultures of sediment bacteria or lake sediments were added to the sediment side of a diffusion chamber. Cellular accumulation of cadmium by cadmium-sensitive and cadmium-resistant bacteria removed between 20% and 80% of the dissolved cadmium from the simulated water column and pore water. Cellular accumulation of cadmium was greatest for cadmium-sensitive isolates that were tested. Sediment with an intact microbial community sequestered 80% of the cadmium added to sediment, whereas autoclaved sediment retained 97% of the metal that was added. Addition of glucose to cadmium-amended sediment decreased retention of cadmium by untreated and autoclaved sediments, resulting in elevated concentrations of dissolved cadmium in the simulated water column.
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    Oligotrophic bacteria in ultra-pure water systems: Media selection and process component evaluations (1991)
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 223-227 
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    ISSN: 1476-5535
    Keywords: Deionized water ; Ultra-pure water ; Ozone ; Ultra-violet sterilization ; Oligotroph ; Bacteria ; R2A medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Presently, tryptic soy agar (TSA) medium is used in the semiconductor industry to determine the concentration of viable oligotrophic bacteria in ultra-pure water systems. Deionized water from an ultra-pure water pilot plant was evaluated for bacterial growth at specific locations, using a non-selective medium (R2A) designed to detect injured heterotrophic as well as oligotrophic bacteria. Results were compared to those obtained using Tryptic Soy Agar. Statistically greater numbers of bacteria were observed when R2A was used as the growth medium. Total viable bacterial numbers were compared both before and after each treatment step of the recirculating loop to determine their effectiveness in removing bacteria. The reduction in bacterial numbers for the reverse osmosis unit, the ion exchange bed, and the ultraviolet sterilizer were 97.4%, 31.3%, and 72.8%, respectively, using TSA medium, and 98.4%, 78.4%, and 35.8% using R2A medium. The number of viable bacteria increased by 60.7% based on TSA medium and 15.7% based on R2A medium after passage of the water through an in-line 0.2-μm pore size nylon filter, probably because of the growth of bacteria on the filter. Our results suggest that R2A medium may give a better representation of the microbial water quality in ultra-pure water systems and therefore a better idea of the effectiveness of the various treatment processes in the control of bacteria.
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    Adaptation of hybridoma cells to higher ammonia concentration (1991)
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    Cytotechnology 7 (1991), S. 103-112 
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    ISSN: 1573-0778
    Keywords: adaptation ; ammonia ; hybridoma ; continuous culture ; serum-free medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Using two mouse-mouse hybridoma cell lines, the response to ammonia step and serial changes was investigated in batch and continuous cultures with serum-free medium. The inhibitory effect of ammonia on cell growth depended on the cultivation mode, and differed markedly between cell lines. The cell line, 4C10B6 producing IgG monoclonal antibody against Pseudomonas, showed a high adaptation ability to ammonia. The 4C10B6 cells could grow under ammonia concentration as high as 21 mmol/l NH4Cl with a viability of 80% in the continuous culture with serial increase in ammonia concentration. Whereas, in the batch culture with ammonia step change the cell growth completely ceased at 12 mmol/l NH4Cl. The other cell line, TO-405 producing IgG monoclonal antibody against hepatitis B surface antigen, could not adapt to ammonia, and the cell growth did not occur at 9 mmol/l NH4Cl even under the ammonia serial change.
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    Age and sex differences in the impact of seasonal energy stress among Andean agriculturalists (1991)
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    Human ecology 19 (1991), S. 351-368 
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    ISSN: 1572-9915
    Keywords: energetics ; seasonality ; nutritional status ; adaptation ; Peru
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract It has been widely argued that children and females are most severely affected during periods of food scarcity. This proposition is tested using dietary and anthropometric data from the Andean community of Nuñoa, Peru. Contrary to expectation, children (ages 12 years and under) are relatively protected from seasonal food scarcity while adults experience severe caloric stress. Anthropometric measures of nutritional status corroborate the dietary analysis, indicating significantly better nutritional status in children. Sex differences in dietary adequacy are not evident. Adult males, however, have significantly poorer measures of nutritional status than adult females. These differences in dietary adequacy and nutritional status reflect adaptations to marked seasonality in work demands and energy availability.
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    Bacterial conjugation betweenEscherichia coli andPseudomonas spp. donor and recipient cells in soil (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 79-84 
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    ISSN: 1476-5535
    Keywords: Bacteria ; Soil ; Conjugation ; Gene transfer ; Plasmids ; Survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Experiments conducted in microcosms containing loam soil samples inoculated with eitherE. coli orPseudomonas spp. donor and recipient cells showed that bacterial cells survived and conjugated over a 24-h incubation period.E. coli transconjugants were detected 6 h after donor and recipient strains were introduced into sterile soil samples. In non-sterile soil samples, transconjugants were detected between 8 and 24 h incubation.Pseudomonas transconjugants were recovered from sterile soil samples between 6 and 12 h after their introduction and as early as 2 h in non-sterile soil. The results show that genetic interactions occur in non-sterile soil in relatively short periods of time at relatively high transfer frequencies (10−3 to 10−4). Studies on genetic interactions in soil are becoming necessary in risk assessment/environmental impact studies prior to the release of genetically engineered or modified organisms into uncontained environments.
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    Production of extracellular emulsifying agent byPseudomonas aeruginosa UG1 (1990)
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    Journal of industrial microbiology and biotechnology 5 (1990), S. 25-31 
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    ISSN: 1476-5535
    Keywords: Oil ; Emulsifier ; Bacteria ; Pseudomonas aeruginosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Twenty-three bacterial strains were isolated from oil-contaminated soil samples. Of these, 20 displayed some ability to effect oil dispersion and they were screened quantitatively for the ability to emulsify 0.5% (v/v) reference oil. One strain, identified asPseudomonas aeruginosa UG1, produced extracellular material that emulsified reference oil, hexadecane and 2-methylnaphthalene at concentrations as high as 6% (v/v) in nutrient broth. Emulsification activity increased during a 10 day incubation period at 30°C. The activity was not influenced by pH over the range 5 to 9. The emulsifying agent was precipitated by cold ethanol. The highest emulsifying activity was detected in the extracellular fraction precipitated between 30 and 50% (v/v) ethanol. A linear relationship was observed between emulsifier concentration (mg/ml) and emulsifying activity. Genetic analysis showed that thePseudomonas aeruginosa UG1 strain did not carry extrachromosomal plasmids, suggesting that the gene(s) coding for emulsifying activity was carried on the chromosome.
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    Establishment of Namalva cell lines which grow continuously in glutamine-free medium (1988)
    Springer
    Cytotechnology 1 (1988), S. 151-158 
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    ISSN: 1573-0778
    Keywords: adaptation ; ammonia ; glutamine-free ; glutamine synthetase ; Namalva cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Glutamine has been shown to be a preferred energy source for some established cell lines and cancer cells in culture (Kovacevic, 1971; Kovacevic, 1972; Lavietes, 1974). Empirically, glutamine is the most abundant amino acid in most of the culture media developed. The major end product of glutamine metabolism is ammonia. Ammonia build up is one of the limiting factors in the proliferation of mammalian cells in higher density culture and is directly related to the initial glutamine concentration. The susceptibility of glutamine to thermodecomposition prevents the heat sterilization of glutamine-enriched media and this significantly increases the cost of medium preparation at the industrial scale. In an attempt to overcome these drawbacks, a population of Namalva cells capable of growing in glutamine-free media was established. The adapted cells were found to contain a higher level of glutamine synthetase activity which enable them to synthesize sufficient amounts of glutamine for their growth.
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    Three hundred years of Taungya: A sustainable system of forestry in south China (1988)
    Springer
    Human ecology 16 (1988), S. 361-376 
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    ISSN: 1572-9915
    Keywords: adaptation ; China ; forestry ; land use ; minorities ; taungya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract Taungya is a system of forest management in which land is cleared and planted initially to food crops. Seedlings of desirable tree species are then planted on the same plot, leading in time to a harvestable stand of timber. Taungya is believed to have been developed by the British in Burma during the nineteenth century. Historical research indicates that successional systems of forest management which follow the pattern of taungya have been used for at least three centuries by ethnic minorities in and by the Han population. The resilience of these systems is associated with economic and social factors which have made the cultivation of trees an adaptive strategy of land use for the inhabitants of the highlands of southern China.
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    Distribution of plasmids in groundwater bacteria (1987)
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    Journal of industrial microbiology and biotechnology 1 (1987), S. 311-317 
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    ISSN: 1476-5535
    Keywords: Plasmid ; Groundwater ; Aquifer ; Bacteria ; Contamination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Bacteria isolated from groundwater aquifer core materials of pristine aquifers at Lula and Pickett, OK, and from a site with a history of aromatic hydrocarbon contamination and natural renovation located at Conroe, TX, were screened for the presence of plasmid DNA by alkaline or enzyme lysis and agarose gel techniques. Some of the isolates were also subjected to taxonomic tests in addition to screening for resistance to antibiotics, tolerance to heavy metal salts, and bacteriocin production. There was no significant difference in the distribution of the traits usually associated with plasmid occurrence in isolates from the three sites. These traits, which occurred at low frequencies, were not restricted to plasmid-bearing strains of the communities. Plasmids were found in isolates from all three sites, but on the average there was a significantly higher percentage of isolates containing plasmids in the samples from Conroe (19.4%) than from either Lula (1.8%) or Pickett (7.7%). The sizes of the plasmids found ranged between 3.5 and 202 kilobases but, for the Conroe samples, many more isolates (67%) contained smaller plasmids (〈10 kb) rather than larger ones. No plasmids were found in bacteria recovered from naturally renovated aquifer material at the Conroe site.
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    Solubilization of coal by an extracellular product fromStreptomyces setonii 75Vi2 (1987)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1987), S. 371-375 
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    ISSN: 1476-5535
    Keywords: Coal ; Coal solubilization ; Streptomyces ; Bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Several low-ranked coals were solubilized when placed on the surface of agar cultures ofStreptomyces viridosporous T7A andS. setonii 75Vi2. When grown in submerged cultureS. setonii 75Vi2 produced an extracellular component that was capable of solubilizing coals. The extracellular coal solubilizing component had a molecular weight of 〈10000 and was heat stable since, after 1h at 121°C, only 30–40% of the activity was lost. Treatment with any of three proteases also appeared to be ineffective in decreasing activity. These results suggest that coal solubilization byS. setonii 75Vi2 is nonenzymatic.
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    High-productivity fermentation process for cultivating industrial microorganisms (1987)
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    Journal of industrial microbiology and biotechnology 2 (1987), S. 79-85 
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    ISSN: 1476-5535
    Keywords: Yeast ; Bacteria ; High cell density ; Oxygen transfer ; Heat transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary High-productivity continuous fermentation processes have been developed for the production of important industrial microorganisms in specially designed fermentors.Saccharomyces cerevisiae, Pichia pastoris, Kluyveromyces fragilis, andCandida utilis yeasts have been grown in bench-scale fermentors at cell densities of over 120 g/l, whileEscherichia coli, Bacillus megaterium, Methylomonas sp. andPseudomonas putida bacteria have been cultivated to cell densities of more than 110 g/l. Productivities (g cells per 1 per h) greater than 25 have been achieved in both bench-scale and 1500-liter fermentors with yeasts, and values as high as 55 have been achieved with bacteria in the bench-scale fermentor. The microorganisms were grown on defined media using ammonia for pH control and as nitrogen source. The fermentor, capable of high oxygen and heat transfer rates, was operated at constant volume with continuous feed and product discharge. The high-productivity process reduces fermentor size, media sterilization requirements, and may under some circumstances eliminate waste and recycle streams. It can also be applied to a variety of biological products.
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    Extracellular amylase production by cassava-fermenting bacteria (1987)
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    Journal of industrial microbiology and biotechnology 2 (1987), S. 123-127 
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    ISSN: 1476-5535
    Keywords: Cassava ; Starch ; Amylase ; Bacteria ; Temperature ; pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Fermentation of cassava tubers was accompanied by a gradual decrease in pH, increased amylase activity in the steep liquor, and increased microbial load and lactic acid concentration. Amylase-producing bacterial strains associated with cassava fermentation were isolated and identified asBacillus subtilis, Bacillus licheniformis andBacillus cereus. The pH optima for the partially purified enzymes of these organisms were 7.0, 5.5 and 7.5, whilst their temperature optima were 30, 37 and 80°C. There was no significant difference in amylase activities when starch, dextrin, amylopectin, glucose and maltose were used as growth substrates.
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    Cultural and genetic adaptations to malaria: Problems of comparison (1986)
    Springer
    Human ecology 14 (1986), S. 311-332 
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    ISSN: 1572-9915
    Keywords: adaptation ; malaria ; Sardinia ; thalassemia ; G-6-Pd deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract The concept of adaptation has been used differently in studies of biological and cultural evolution, and this divergence raises the question of whether genetic and cultural adaptations are truly comparable. This paper compares genetic and cultural traits associated with endemic malaria in Sardinia, Italy. Thalassemia and G-6-Pd deficiency, two genetic traits of the Island's population, are believed to enhance fitness against malaria, despite increased risk for the diseases of thalassemia major and favism. Two cultural traits, a pastoral pattern of inverse transhumance and rules limiting the geographical mobility of lowland women, limited exposure to the malaria vector, Anopheles labranchiae; these are used as examples of cultural adaptations. The distribution, costs, and benefits of the adaptive cultural and genetic traits are compared, and the theoretical difficulties of finding a common measure of adaptive value are discussed.
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    URL: http://dx.doi.org/10.1007/BF00889033
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    Bicultural oscillation as a long-term adaptation to cultural frontiers: Cases and questions (1985)
    Springer
    Human ecology 13 (1985), S. 411-432 
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    ISSN: 1572-9915
    Keywords: adaptation ; cyclical change ; cultural boundary ; versatility ; nomadism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract Comparison of two nomadic peoples, one pastoral, the other food-collecting, reveals that rapid bicultural oscillation of both is an adaptation to their multizoned environments. One may speak of their being flexible in the face of temporally variable environmental hazards and opportunities. For each people, the more nomadic of their two cultures is associated with norms of propriety, the more sedentary one entails what Freilich calls “smart norms.” It is argued that this complementation gives both cultures continuing appeal, thereby facilitating rapid change. A major difference between decisionmaking mechanisms in the two cases underscores that we treat a system type in a very abstract sense. Other, possibly similar cases are noted from ethnographic literature. A formal theory is put forward concerning the tendency of people under certain conditions to develop oscillating biculturalism. Such rapidly oscillating systems are proposed to have a biological analogue in versatile acclimatizers.
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    URL: http://dx.doi.org/10.1007/BF01531153
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    Ciguatera — Parameters of a tropical health problem (1984)
    Springer
    Human ecology 12 (1984), S. 253-273 
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    ISSN: 1572-9915
    Keywords: ciguatera ; fish poisoning ; marine biotoxins ; ciguatoxin ; neurotoxins ; dinoflagellates ; marine resources ; Pacific Islands ; health ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract Ciguatera fish poisoning is a significant health and resource problem in the tropical world, largely because of its uneven and unpredictable distribution in space and through time. Here, the problem is reviewed with evidence from the Pacific Basin. The contemporary distribution of ciguatera and the species commonly perceived to be toxic are considered and a hypothesis relating the greater prevalence of ciguatera in the eastern Pacific to reduced species diversity is presented. The problem is also considered as a public health phenomenon (the mean reported incidence for the Pacific region as a whole in 1981 was 109/100,000) and attention is given to island dwellers' adaptation to the problem, their explanations of its etiology, as well as its detection, prophylaxis, and cure.
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    URL: http://dx.doi.org/10.1007/BF01540591
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    Monoculture, polyculture, and polyvariety in tropical forest Swidden cultivation (1983)
    Springer
    Human ecology 11 (1983), S. 13-34 
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    ISSN: 1572-9915
    Keywords: Swidden cultivation ; Ye'kwana ; Yanomamö ; neotropics ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract A number of researchers have suggested that polyculture is characteristic of native tropical forest swiddens and have adduced theory from community ecology to account for its adaptiveness. Ye'kwana and Yanomamö swidden cultivation is examined, and it is shown that polyculture is not practiced to any significant degree. Instead, the concept of polyvariety is introduced along with a number of other cultivation practices that more simply account for the adaptiveness of Ye'kwana and Yanomamö gardening. In addition, comparative data from other parts of the tropical world indicate that polyculture is no more common than monoculture and recent advances in ecological research indicate that the diversity-stability hypothesis that underpins adaptive arguments of polyculture is in need of drastic revision.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00891228
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    Interactions of genetic and cultural evolution: Models and examples (1982)
    Springer
    Human ecology 10 (1982), S. 289-323 
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    ISSN: 1572-9915
    Keywords: coevolution ; adaptation ; cultural evolution ; genetic evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract This paper proposes models and examples of five principal modes of interaction between genes and culture in human evolution. Because genes and culture ultimately interact in the minds of individuals, the models are focused on individual level processes of “constrained microevolution.” The central hypotheses are (1) that cultural evolution as well as genetic evolution commonly proceeds by the differential transmission of alternative “instructions” among individuals, (2) that genetic and cultural processes directly interact through mutual influence on each other's differentials of transmission in a population, (3) that the cultural process is often self-selecting by its own criteria, and (4) that these criteria generally operate to enhance rather than oppose human adaptation. Evolutionary change at higher levels, which is particularly important in sociocultural evolution, is interpreted as restructuring the nature and extent of the variability available at the individual level. To clarify the conceptual differences of the models and hopefully to stimulate related analyses in other areas, I discuss selected examples of each of these interactions. I conclude with some remarks on the relative importance of the models to human ecology and evolution.
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    URL: http://dx.doi.org/10.1007/BF01531188
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    The ecology of marine exploitation in prehistoric Hawaii (1982)
    Springer
    Human ecology 10 (1982), S. 455-476 
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    ISSN: 1572-9915
    Keywords: fishing ; faunal analysis ; Oceania (Hawaii) ; niche width ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract Archaeological evidence for prehistoric strategies of marine exploitation in Oceania may be profitably analyzed from an ecological perspective, in which individual sites and assemblages are viewed in the context of adaptation to local environmental constraints. This perspective is illustrated through the contrastive analysis of environment, technology, and faunal remains at three prehistoric Hawaiian sites. Differing strategies of marine exploitation evidenced for each site are shown to reflect local marine environmental conditions. An ecological approach shows greater promise for an understanding of prehistoric adaptation to marine environment than the typological analyses current in much archaeological work on fishing.
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    URL: http://dx.doi.org/10.1007/BF01531167
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    Environmental analysis in human evolution and adaptation research (1980)
    Springer
    Human ecology 8 (1980), S. 135-170 
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    ISSN: 1572-9915
    Keywords: cultural ecology ; adaptation ; environmental analysis ; human evolution ; evolutionary ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract Human evolution and ecology analyses argue that environment is a major factor influencing biological and sociocultural adaptation, but they rarely analyze environmental properties. Multiple problems of perspective and method can arise from the normative and nondynamic environmental descriptions which pervade these analyses. This paper examines human adaptation frameworks to identify theoretical guidelines for environmental description in ways appropriate to available theories of biocultural evolution or congruent with known ecosystem qualities. Concepts and terminology are given for describing the spatial and temporal properties characteristic of ecosystems and central to hypotheses about ecological adaptation. These include: patchiness and grain; stability and resilience; persistence and recurrence; and predictability, constancy, and contingency. Field experience, theory, and the qualities of ecosystems themselves suggest that detailed, historical (long-term) environmental analysis is necessary to determine the role of ecological factors in human evolution and adapation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01531439
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    Human adaptation and energetic efficiency (1979)
    Springer
    Human ecology 7 (1979), S. 53-74 
    Details
    ISSN: 1572-9915
    Keywords: energetic efficiency ; adaptation ; behavioral ecology ; optimal foraging theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract A lack of consensus on the general adaptive significance of energetic efficiency can be shown to exist in ecology and anthropology. After briefly reviewing key studies in optimal foraging theory and ecological anthropology, a model is presented which includes the following elements: (1) an equation of adaptive success with reproductive fitness, within an optimality framework; (2) a definition of energy limitation consistent with this framework; (3) a distinction between efficiency of energy capture and efficiency of energy use in achieving other goals; (4) a multiple definition of energetic efficiency that distinguishes purely energetic measures (output/input) from rate measures (energy captured per unit time); (5) the inclusion of time budgeting as a primary adaptive constraint; (6) a quantitative demonstration that increased output/input ratios do not consistently predict an increase in net energy captured, and are poor measures where time is a constraint. The general conclusion is that where energy is limiting, increased efficiency in the rate of energy capture will be adaptive because more net energy will be made available; where energy is not limiting, an increased net capture rate may still confer increased adaptive success, since time and labor energy are freed from energy-capture activities and can be devoted to achieving other adaptive goals. But while energetic efficiency, properly defined, is shown to have general adaptive significance in all cases where time or energy are constraints, considerations of adaptive optimality preclude the general equation of energetic efficiency and adaptive success.
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    URL: http://dx.doi.org/10.1007/BF00889352
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