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  • Articles  (17)
  • genetic diversity  (17)
  • 2010-2014
  • 2000-2004  (17)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (17)
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  • Articles  (17)
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  • 1
    Electronic Resource
    Electronic Resource
    AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.) (2000)
    Springer
    Molecular breeding 6 (2000), S. 125-134 
    Details
    ISSN: 1572-9788
    Keywords: ryegrass ; gene pool ; AFLP fingerprinting ; genetic distance ; genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An evaluation was performed of the potential use of AFLP markers to reveal polymorphisms among Lolium perenne plants with different degrees of kinship. Radioactive and fluorescent detection techniques were applied. The use of a fluorescent detection approach contributed greatly to the speed and ease of conducting and interpreting the AFLP patterns. The great discriminative power of AFLP markers and their capacity to represent genetic relationships among ryegrass plants was shown. Despite the high polymorphic value of the AFLP markers, standard statistical tests could not differentiate between two gene pools derived from different breeding programmes. It proved also impossible to correlate fodder and turf phenotypes with AFLP distance data. A very important point revealed by our data is the high degree of genetic diversity within commercial ryegrass varieties. Our findings are relevant to any outcrossing crop with a breeding strategy based on the production of synthetic populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009680614564
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  • 2
    Electronic Resource
    Electronic Resource
    Further characterization of AFLP® data as a tool in genetic diversity assessments among maize (Zea mays L.) inbred lines (2000)
    Springer
    Molecular breeding 6 (2000), S. 265-276 
    Details
    ISSN: 1572-9788
    Keywords: AFLP® ; genetic diversity ; methylation AFLP® ; polymorphism information content ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract AFLP® markers generated by CNG methylation sensitive (PstI/MseI) and CNG methylation insensitive (EcoRI/MseI) enzyme combinations and AFLP markers collected from hypomethylated (PstI/MseI) and hypermethylated (m PstI/MseI) regions were compared for their polymorphism information content, sampling variance and patterns of genetic diversity in a representative sample of 33 inbred lines of maize (Zea mays L.). We demonstrate that the mean polymorphism information content generated by sets of PstI/MseI and m PstI/MseI markers (0.38) is significantly higher than by sets ofEcoRI/MseI markers (0.33). Also the sampling variance highlighted the distinctive nature of the (m) PstI/MseI markers: to achieve a mean standard deviation of 5% in the estimation of genetic distance among the 33 inbreds, the PstI/MseI and m PstI/MseI marker sets (135 and 129 markers, respectively) are clearly smaller than the EcoRI/MseI marker set (173 markers). A further minimizing of the sampling variance of AFLP data in the estimation of genetic similarities was obtained by reducing marker information redundancy by selecting markers evenly distributed over each chromosome: a set of only 106 AFLP markers, sampled conditionally on their genetic map position, was required for a mean standard deviation of 5% in the estimation of genetic distance among the 33 inbreds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009656422272
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic diversity of the `peruvianum-complex' (Lycopersicon peruvianum (L.) Mill. and L. chilense Dun.) revealed by RAPD analysis (2000)
    Springer
    Euphytica 116 (2000), S. 23-31 
    Details
    ISSN: 1573-5060
    Keywords: cluster analysis ; genetic distance ; genetic diversity ; Lycopersicon ; peruvianum-complex' ; RAPD analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract For further genetic improvement of the tomato, it is essential to promote efficient collection, classification, preservation and utilization of the wild tomato species. To investigate the genetic diversity of the `peruvianum-complex' (PC) species of highly polymorphic wild tomato relatives and the genetic relationship between the PC and the`esculentum-complex' (EC) species including the cultivated, an RAPD analysis was carried out. A total of 435 RAPDs were obtained from 50 accessions of all the nine Lycopersicon species using only 10random primers. Average genetic distances among theL. peruvianum accessions and among L. chilense accessions were larger than in any species of the `esculentum-complex' (EC). In addition, the cluster analysis conducted by using the neighbor joining method showed that all the tested accessions were clearly divided into at least four main clusters consisting of the PC, the self-compatible EC, L. pennellii and L. hirsutum. This study demonstrated that the PC species had the largest genetic diversity in the Lycopersicon, and the genetic background of the PC was clearly different from those of the self-compatible EC species including the cultivated tomato. Moreover, it was suggested that the L. peruvianum accessions in northern Peru and the L. chilense accessions in southern Peru might have larger genetic variation in each species. From these results, it was concluded that the PC with such high and different genetic variation would have a potentiality to supply more and useful traits that are unknown at present for further tomato breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004064909631
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of the genetic diversity among elite tea (Camellia sinensis var. sinensis) accessions using RAPD markers (2000)
    Springer
    Euphytica 115 (2000), S. 7-16 
    Details
    ISSN: 1573-5060
    Keywords: Camellia sinensis ; genetic diversity ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The diversity of 27 superior tea (Camellia sinensis var. sinensis) accessions from Korea, Japan and Taiwan was examined with RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) markers. Out of the 50 primers screened, 17 primers generated 58 polymorphic and reproducible bands. A minimum of 3 primers was sufficient to distinguish all the 27 accessions studied. The Shannon's index used to partition diversity into inter- and intra-group, revealed that 71 percent of variability resided within groups and 29 percent between groups. Diversity was greatest within the Korean group followed by Taiwan and Japan. The relatively high diversity observed in Korea might reflect the larger genetic base of its plantations while the low diversity in Japan could be explained by the long and intensive tea selection programme in this country. A dendrogram based on the UPGMA-link method using Jaccard's distances and multivariate Factorial correspondence analysis clustered the tea accessions into two main groups, regrouping the Taiwan cultivars on the one side and the Korean and Japanese accessions on the other side. This suggests that the Taiwan tea studied here may have a different origin from that of Korea and Japan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003939120048
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  • 5
    Electronic Resource
    Electronic Resource
    Development of an algorithm identifying maximally diverse core collections (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 515-526 
    Details
    ISSN: 1573-5109
    Keywords: Capsicum ; core collection ; genetic diversity ; RAPD ; Theobroma cacao
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The development of a core collection, one which represents the genetic diversity of a crop with minimal redundancy and increases utility of the collection as a whole, is especially important as the funding for germplasm collections decreases. With limited resources, it is difficult to manage large germplasm collections and disperse genetically diverse germplasm to plant breeders. An algorithm was developed to assist in selection of core collections based on estimates of genetic distance. The criteria for selection of the maximum genetically diverse set were based on rankings of genetic distance between an accession with respect to all other accessions. Depending on the size core which a user wished, a zone around each selected accession was determined and no other accession within these limits was selected. The premise for the algorithm was that the genetic variability represented in the core must be representative of the distribution of genetic distances within the population of interest. In the present study, the algorithm was used with RAPD-marker-based estimates of genetic distance for 270 Theobroma cacao L. accessions and 134 Capsicum accessions that chose a set representing 18.5% of the population and representing the breadth of RAPD-based variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008784610962
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  • 6
    Electronic Resource
    Electronic Resource
    Assessing genetic diversity of sweet potato (Ipomoea batatas (L.) Lam.) cultivars from tropical America using AFLP (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 659-665 
    Details
    ISSN: 1573-5109
    Keywords: AFLP ; gene banks ; genetic diversity ; germplasm ; Ipomoea batatas ; sweet potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The sweet potato genebank at the International Potato Center (CIP) maintains 5,526 cultivated I. batatas accessions from 57 countries. Knowledge of the genetic structure in this collection is essential for rational germplasm conservation and utilization. Sixty-nine sweet potato cultivars from 4 geographical regions (including 13 countries) of Latin America were randomly sampled and fingerprinted using AFLP markers. A total of 210 polymorphic and clearly scorable fragments were generated. A geographic pattern of diversity distribution was revealed by mean similarity, multidimensional scaling (MDS), and analysis of molecular variance (AMOVA). The highest genetic diversity was found in Central America, whereas the lowest was in Peru-Ecuador. The within-region variation was the major source of molecular variance. The between-regions variation, although it only explains 10.0% of the total diversity, is statistically significant. Cultivars from Peru-Ecuador, with the lowest level of within region diversity, made the most significant contribution to the between region differentiation. These results support the hypothesis that Central America is the primary center of diversity and most likely the center of origin of sweet potato. Peru-Ecuador should be considered as a secondary center of sweet potato diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026520507223
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  • 7
    Electronic Resource
    Electronic Resource
    Enzyme polymorphism and adaptation in strawberry clover (Trifolium fragiferum L.) (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 197-205 
    Details
    ISSN: 1573-5109
    Keywords: allozymes ; climate ; environment ; genetic diversity ; genetic structure ; Trifolium fragiferum L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Enzymatic survey of eight loci in 22 populations of Trifolium fragiferum L. sampled in three regions in Poland showed regional differences with regard to five genetic parameters describing genetic polymorphism and variability. Differences were attributed to the following climatic variables: annual precipitation, temperature and solar radiation. Continental climatic conditions were favorable for accumulation of genetic polymorphism and variability. Vegetative reproduction versus sexual reproduction had a higher incidence rate under milder climatic conditions. This former mode of reproduction also affected patterns of distribution of variation within regions, by reducing the intra-populational component of total variability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008703226642
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  • 8
    Electronic Resource
    Electronic Resource
    Preliminary study of genetic diversity in Swedish flax (Linum usitatissimum) (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 417-424 
    Details
    ISSN: 1573-5109
    Keywords: allozymes ; flax ; genetic diversity ; Linum usitatissimum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To investigate the genetic diversity of Linum usitatissimumL. in Sweden, 18 accessions, including 13 cultivars and five landraces, were analysed. This study was based on genetic variation in three enzyme systems (i.e., PGD, GPI and MDH) by using horizontal starch gel electrophoresis. The total genetic diversity of the studied flax material was very high (H T= 0.62). Even though the highest genetic diversity lies within the accessions (G ST= 0.07), a clear differentiation between fibre and oil flax was found with respect to three polymorphic loci (Pgd-1, Gpi-2 and Mdh-1). A phenogram, based on Nei's genetic distances between the accessions studied, showed five clearly defined groups but with low variation within the groups. The unexpected high genetic diversity found within accessions in the studied flax material may indicate that flax is more outbreeding than earlier believed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008721528588
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  • 9
    Electronic Resource
    Electronic Resource
    Microsatellite markers – a new tool for distinguishing diploid wheat species (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 497-505 
    Details
    ISSN: 1573-5109
    Keywords: diploid wheats ; genetic diversity ; Triticum baeoticum ; Triticum monococcum ; Triticum urartu ; wheat microsatellites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Triticum baeoticum and T. urartu are very similar morphologically. By using microsatellite markers it was possible to distinguish between these two species. Microsatellite markers are, therefore, a powerful new tool to support the determination of critical races in diploid wild wheat species. They also allow the discussion of evolutionary pathways within Triticum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008761928959
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic diversity in European accessions of the Barley Core Collection as detected by isozyme electrophoresis (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 571-581 
    Details
    ISSN: 1573-5109
    Keywords: allelic variation ; barley ; core collection ; genetic diversity ; isozyme loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic diversity in 79 European accessions of the Barley Core Collections was surveyed using isozyme electrophoresis. A total of 26 alleles were observed at the ten isozyme loci. All loci were polymorphic except Pgd-1 which was monomorphic. The comparison of the results with those of previous studies indicates that most of the alleles occurring in the European Barley are also observed in this set of the European Barley Core Collections. Only five alleles (Est-1 Al; Est-5 Ag, Te; Pgi-1 C and Ndh-2 B) were absent. Nine of 26 alleles were rare alleles, which were detected only in one or two accessions. Moreover, most of rare alleles were detected in 6-rowed winter barley. It is very important to include rare alleles for maximising the genetic variations in core collections. In the set of European Barley Core Collection, 6-rowed barley contained larger diversity than 2-rowed barley; winter type contained larger diversity than spring type. The cluster analysis separated 79 accessions into three major groups. Group I is more complex and comprised 2-rowed spring, 2-rowed winter and 6-rowed winter barley. In this group, 18 accessions in the cluster A and 14 accessions in the cluster B possessed identical genotypes as judged from the ten isozyme data. Principal coordinate analysis could not clearly separate the spring cultivars from the winter barley lines, as well as not separate 2-rowed from 6-rowed barley.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026532215990
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  • 11
    Electronic Resource
    Electronic Resource
    Characterization of Mexican wheat landraces using agronomically useful attributes (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 591-602 
    Details
    ISSN: 1573-5109
    Keywords: characterization ; evaluation ; genetic diversity ; genetic resources ; pattern analysis ; sampling strategy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In 1992, 465 individual spikes of bread wheat were collected from 24 sites in three states of Mexico. They were examined for 15 morphological, agronomic and grain quality attributes as part of the routine regeneration process conducted by the CIMMYT Wheat Genetic Resources Program in unreplicated hill plots in a screen house. A pattern analysis (combined use of classification and ordination methods) of the data provided a good description of the accessions and the collection sites. Since economically useful attributes were used the analysis provided relevant information for both potential users and the germplasm curators. Potential users have a description of the accessions from which to choose relevant breeding material and curators can assess how well the accessions represent the diversity in the collection sites. The analysis would not have been possible if the individual spikes from collection sites were bulked as is the common practice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026550624037
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  • 12
    Electronic Resource
    Electronic Resource
    Phylogenetic relationships among species of Citrullus and the placement of C. rehmii De Winter as determined by Internal Transcribed Spacer (ITS) sequence heterogeneity (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 215-222 
    Details
    ISSN: 1573-5109
    Keywords: Citrullus spp. ; genetic diversity ; internal transcribed spacer ; phylogeny ; watermelon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The internal transcribed spacer regions (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA from the four recognized species of Citrullus (C. lanatus var. lanatus (Thunb.) Matsum. & Nakai., C. lanatus var. citroides (Bailey) Mansf., C. colocynthis (L.) Schrad., C. ecirrhosus Cogn., and C. rehmii De Winter) and Acanthosicycos naudinianus (Sond.) C. Jeffrey were amplified by PCR, and direct sequenced. Within the taxa examined, the length of ITS1 varied from 216 bp to 219 bp, and ITS2 varied from 239 bp to 249 bp. The average %CG content ranged from 59 to 64% and from 62 to 66% for ITS1 and ITS2, respectively. The greater length variation observed in ITS2 was primarily attributable to the occurrence of a (CC)n microsatellite. Cladistic (PAUP) and phenetic (MEGA) analyses resulted in highly resolutive trees. ITS sequence analysis placed the recently described C. rehmii adjacent to the cultivated watermelon and supported the validity of the species classification of this taxa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008754719102
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  • 13
    Electronic Resource
    Electronic Resource
    Genetic diversity within and among landraces of pearl millet (Pennisetum glaucum) under farmer management in West Africa (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 561-568 
    Details
    ISSN: 1573-5109
    Keywords: AFLP ; genebanks ; genetic diversity ; landraces ; molecular markers ; pearl millet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of farmer management on pearl millet landrace diversity was determined by evaluating variation in individual farmers' populations from two villages in north-eastern Nigeria. The variability within and between landrace samples was estimated using variation at 163 amplified fragment length polymorphism marker (AFLP) loci. The data indicated that individual farmers' husbandry practices result in the isolation of their own group of ideotypes each in their own unique genetic backgrounds, thereby rendering landrace names inappropriate as indicators of a generic genetic identity. The implications of these findings for sampling strategies for genebanks and regional genetic evaluations are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008767220320
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  • 14
    Electronic Resource
    Electronic Resource
    Genetic diversity in grasspea (Lathyrus sativus L.) (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 163-169 
    Details
    ISSN: 1573-5109
    Keywords: allelic frequency ; genetic distance ; genetic diversity ; isozyme ; Lathyrus sativus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic diversity was investigated in 348 accessions and subaccessions of grasspea (Lathyrus sativus L.) from 10 geographical regions. Polymorphism for 20 isozymes of 13 enzyme systems was studied to estimate the genetic diversity. The Near East and North Africa regions included the most variability for these isozyme systems, suggesting that the center of diversity (center of origin) for grasspea is in this general area. The lowest variability was found in accessions and subaccessions from South America, followed by those from Sudan–Ethiopia. Diversity was measured for individual loci over regions and EST-1 and SKDH had the highest genetic diversity. The closest genetic diversity was observed for LAP-2, followed by AAT-1 and PGM. The closest genetic distance existed between populations from the Near East and North Africa. Populations from South Asia and Sudan–Ethiopia, though geographically widely separated, exhibited a closer genetic distance from each other than from other regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008760604990
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  • 15
    Electronic Resource
    Electronic Resource
    Genetic diversity in tef [Eragrostis tef (Zucc) Trotter] and its relatives as revealed by Random Amplified Polymorphic DNAs (2000)
    Springer
    Euphytica 112 (2000), S. 15-22 
    Details
    ISSN: 1573-5060
    Keywords: Eragrostis curvula ; E. pilosa ; E. tef ; genetic diversity ; RAPD ; Tef
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tef is one of the staple cereal crops in Ethiopia. To evaluate genetic diversity of tef and its relatives, 47 accessions of tef, three accessions of E. pilosa, and six accessions of E. curvulawere analyzed using random amplified polymorphic DNA (RAPD) markers. The level of polymorphism among the wild species was extremely high, while low polymorphism was detected among tef accessions. All cultivars and wild species under study could be distinguished with the help of different primers, thereby indicating the potential of RAPD in the genetic fingerprinting of tef. Accessions from E. curvula and E. pilosa can be differentiated by a single selected primer. In spite of low polymorphism within tef, accessions under study could be distinguished by a combination of selected primers. Cluster analysis indicated that tef is a very closely related species to E. pilosa with 45%similarity, supporting the hypothesis that tef originated from E. pilosa based on morphological data. Given that RAPD are relatively quick, simple to use, and are not subjected to environmental influences, they provide a valuable new approach for the genetic fingerprinting and study of genetic diversity in tef.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003802207158
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  • 16
    Electronic Resource
    Electronic Resource
    Patterns of RFLP-based genetic diversity in germplasm pools of common wheat with different geographical or breeding program origins (2000)
    Springer
    Euphytica 115 (2000), S. 197-208 
    Details
    ISSN: 1573-5060
    Keywords: genetic diversity ; germplasm ; RFLP ; Triticum aestivum ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A set of 292 accessions of common wheat (Triticum aestivum L.) representing 21 germplasm pools based on geographical or breeding program origins was assayed for RFLP diversity. Thirty cDNA and genomic DNA probes and the HindIII restriction enzyme were employed for RFLP analysis. About 61% of all 233 scored bands were present in 75% or more of the accessions. All but one of the 30 probes revealed polymorphism, and the average number of distinct patterns per probe over all accessions was 9.5.Polymorphic Information Content (PIC) values within a pool varied from 0 to 0.9 and depended on the identities of both the germplasm pool and the probe. Rare banding patterns with a relative frequency of ≤0.2 within a pool were detected. These rare patterns were more likely to occur in pools exhibiting high levels of heterogeneity. The highest level of polymorphism was observed in the Turkish landraces from Southwest Asia. The Eastern U.S. soft red winter wheat germplasm pool was more genetically diverse than the other advanced germplasm pools, and nearly as diverse as the Turkish landrace pool. RFLP-based genetic relationships between germplasm pools generally tracked expectations based on common geographical origin, breeding history and/or shared parentages. The Chinese wheat landraces from Sichuan, Tibet, and Yunnan provinces were distinct from other pools. Similarity matrices for among-pool genetic distance estimates based on either band frequencies or banding pattern frequencies showed good correlation with matrices derived from Nei and Li's mean genetic similarity estimates (r=−0.82** and r=−0.73**, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004022601879
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  • 17
    Electronic Resource
    Electronic Resource
    Differentiation of Cotton-defoliating and Nondefoliating Pathotypes of Verticillium dahliae by RAPD and Specific PCR Analyses (2000)
    Springer
    European journal of plant pathology 106 (2000), S. 507-517 
    Details
    ISSN: 1573-8469
    Keywords: Verticillium wilt ; Gossypium hirsutum ; molecular markers ; genetic diversity ; virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Severe Verticillium wilt of cotton in southern Spain is associated with the spread of a highly virulent, defoliating (D) pathotype of Verticillium dahliae. Eleven of the D and 15 of a mildly virulent, nondefoliating (ND) pathotype were analyzed by random amplified polymorphic DNA (RAPD) using the polymerase chain reaction (PCR). Six of 21 primers tested generated pathotype-associated RAPD bands. Another 21 V. dahliae isolates were compared in blind trials both by RAPD-PCR using the six selected primers and pathogenicity tests on cotton cultivars. There was a 100% correlation between pathotype characterization by each method. Unweighted paired group method with arithmetic averages cluster analysis was used to divide the 47 V. dahliae isolates into two clusters that correlated with the D or ND pathotypes. There was more diversity among ND isolates than among D isolates, these latter isolates being almost identical. ND- and D-associated RAPD bands of 2.0 and 1.0 kb, respectively, were cloned, sequenced, and used to design specific primers for the D and ND pathotypes. These pathotype-associated RAPD bands were present only in the genome of the pathotype from which they were amplified, as shown by Southern hybridization. The specific primers amplified only one DNA band of the expected size, and in the correct pathotype, when used for PCR with high annealing temperature. These specific primers successfully characterized V. dahliae cotton isolates from China and California as to D or ND pathotypes, thus demonstrating the validity and wide applicability of the results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008756307969
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