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  • 1
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    Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer (2017)
    Elsevier
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 188–193, doi:10.1016/j.ydbio.2016.03.006.
    Description: Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated that TALENs are able to induce mutations in F0 Xenopus embryos, but none has demonstrated germline transmission of such mutations in X. laevis. In this report we used the oocyte host-transfer method to generate mutations in both tyrosinase homeologs and found highly-penetrant germline mutations; in contrast, embryonic injections yielded few germline mutations. We also compared the distribution of mutations in several F0 somatic tissues and germ cells and found that the majority of mutations in each tissue were different. These results establish that X. laevis J strain animals are very useful for generating germline mutations and that the oocyte host-transfer method is an efficient technique for generating mutations in both homeologs.
    Description: This work was supported by grants from the NIH (OD010997 and HD084409).
    Keywords: Xenopus laevis ; TALENs ; J strain ; Tyrosinase ; Oocyte host-transfer ; Genome editing
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 2
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    Luteinizing Hormone is an effective replacement for hCG to induce ovulation in Xenopus (2017)
    Elsevier
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 442–448, doi:10.1016/j.ydbio.2016.05.028.
    Description: Injection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well as lack of a well tested alternative have resulted in frustrating experimental delays in laboratories that predominantly use Xenopus in their research. Mammalian Luteinizing Hormones (LH) share structural similarity, functional equivalency, and bind the same receptor as hCG; this suggests that LH may serve as a good alternative to hCG for promoting ovulation in Xenopus. LH has been found to induce maturation of Xenopus oocytes in vitro, but whether it can be used to induce ovulation in vivo has not been examined. Here we compared the ability of four mammalian LH proteins, bovine (bLH), human (hLH), ovine (oLH), porcine (pLH), to induce ovulation in Xenopus when injected into the dorsal lymph sac of sexually mature females. We find that both ovine and human LH, but not bovine or porcine, are good substitutes for hCG for induction of ovulation in WT and J strain Xenopus laevis and Xenopus tropicalis.
    Description: This work was supported by a grant from the NIHP40OD010997.
    Keywords: Xenopus laevis ; J strain ; Luteinizing Hormone ; Ovulation ; Chorionic gonadotropin
    Repository Name: Woods Hole Open Access Server
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  • 3
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    Expanding the genetic toolkit in Xenopus : approaches and opportunities for human disease modeling (2017)
    Elsevier
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    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 325-335, doi:10.1016/j.ydbio.2016.04.009.
    Description: The amphibian model Xenopus, has been used extensively over the past century to study multiple aspects of cell and developmental biology. Xenopus offers advantages of a non-mammalian system, including high fecundity, external development, and simple housing requirements, with additional advantages of large embryos, highly conserved developmental processes, and close evolutionary relationship to higher vertebrates. There are two main species of Xenopus used in biomedical research, Xenopus laevis and Xenopus tropicalis; the common perception is that both species are excellent models for embryological and cell biological studies, but only Xenopus tropicalis is useful as a genetic model. The recent completion of the Xenopus laevis genome sequence combined with implementation of genome editing tools, such as TALENs (transcription activator-like effector nucleases) and CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated nucleases), greatly facilitates the use of both Xenopus laevis and Xenopus tropicalis for understanding gene function in development and disease. In this paper, we review recent advances made in Xenopus laevis and Xenopus tropicalis with TALENs and CRISPR-Cas and discuss the various approaches that have been used to generate knockout and knock-in animals in both species. These advances show that both Xenopus species are useful for genetic approaches and in particular counters the notion that Xenopus laevis is not amenable to genetic manipulations.
    Description: This work was supported by the National Institutes of Health (P40 OD010997 to M.E.H., R01 HD084409 to M.E.H., R01 HL112618 to P.T. and F.C., and R01 HL127640 to P.T. and F.C.; and the U.S. Environmental Protection Agency (G11E10367 to D.F.).
    Keywords: CRISPR-Cas ; TALENs ; J strain ; Xenopus laevis ; Xenopus tropicalis ; Knock-in ; Human disease model
    Repository Name: Woods Hole Open Access Server
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  • 4
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    Emerging trends in the sea state of the Beaufort and Chukchi seas (2016)
    Elsevier
    Details
    Publication Date: 2022-05-26
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ocean Modelling 105 (2016): 1-12, doi:10.1016/j.ocemod.2016.02.009
    Description: The sea state of the Beaufort and Chukchi seas is controlled by the wind forcing and the amount of ice-free water available to generate surface waves. Clear trends in the annual duration of the open water season and in the extent of the seasonal sea ice minimum suggest that the sea state should be increasing, independent of changes in the wind forcing. Wave model hindcasts from four selected years spanning recent conditions are consistent with this expectation. In particular, larger waves are more common in years with less summer sea ice and/or a longer open water season, and peak wave periods are generally longer. The increase in wave energy may affect both the coastal zones and the remaining summer ice pack, as well as delay the autumn ice-edge advance. However, trends in the amount of wave energy impinging on the ice-edge are inconclusive, and the associated processes, especially in the autumn period of new ice formation, have yet to be well-described by in situ observations. There is an implicit trend and evidence for increasing wave energy along the coast of northern Alaska, and this coastal signal is corroborated by satellite altimeter estimates of wave energy.
    Description: This work was supported by the Office of Naval Research, Code 322, “Arctic and Global Prediction”, directed by Drs. Martin Jeffries and Scott Harper. (Grant numbers and Principal Investigators are: Ackley, N000141310435; Babanin, N000141310278; Doble, N000141310290; Fairall, N0001413IP20046; Gemmrich, N000141310280; Girard-Ardhuin and Ardhuin, N000141612376; Graber, N000141310288; Guest, N0001413WX20830; Holt, N0001413IP20050; Lehner, N000141310303; Maksym, N000141310446; Perrie, N00014-15-1-2611; Rogers, N0001413WX20825; Shen, N000141310294; Squire, N000141310279; Stammerjohn, N000141310434; Thomson, N000141310284; Wadhams, N000141310289.)
    Keywords: Sea ice ; Arctic Ocean ; Ocean surface waves
    Repository Name: Woods Hole Open Access Server
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  • 5
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    The influence of sea ice and snow cover and nutrient availability on the formation of massive under-ice phytoplankton blooms in the Chukchi Sea (2015)
    Elsevier
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Deep Sea Research Part II: Topical Studies in Oceanography 118 (2015): 122-135, doi:10.1016/j.dsr2.2015.02.008.
    Description: A coupled biophysical model is used to examine the impact of changes in sea ice and snow cover and nutrient availability on the formation of massive under-ice phytoplankton blooms (MUPBs) in the Chukchi Sea of the Arctic Ocean over the period 1988–2013. The model is able to reproduce the basic features of the ICESCAPE (Impacts of Climate on EcoSystems and Chemistry of the Arctic Pacific Environment) observed MUPB during July 2011. The simulated MUPBs occur every year during 1988–2013, mainly in between mid-June and mid-July. While the simulated under-ice blooms of moderate magnitude are widespread in the Chukchi Sea, MUPBs are less so. On average, the area fraction of MUPBs in the ice-covered areas of the Chukchi Sea during June and July is about 8%, which has been increasing at a rate of 2% yr–1 over 1988–2013. The simulated increase in the area fraction as well as primary productivity and chlorophyll a biomass is linked to an increase in light availability, in response to a decrease in sea ice and snow cover, and an increase in nutrient availability in the upper 100 m of the ocean, in conjunction with an intensification of ocean circulation. Simulated MUPBs are temporally sporadic and spatially patchy because of strong spatiotemporal variations of light and nutrient availability. However, as observed during ICESCAPE, there is a high likelihood that MUPBs may form at the shelf break, where the model simulates enhanced nutrient concentration that is seldom depleted between mid-June and mid-July because of generally robust shelf-break upwelling and other dynamic ocean processes. The occurrence of MUPBs at the shelf break is more frequent in the past decade than in the earlier period because of elevated light availability there. It may be even more frequent in the future if the sea ice and snow cover continues to decline such that light is more available at the shelf break to further boost the formation of MUPBs there.
    Description: This work is supported by the NASA Cryosphere Program and Climate and Biological Response Program and the NSF Office of Polar Programs (Grant Nos. NNX12AB31G; NNX11AO91G; ARC-0901987).
    Keywords: Arctic Ocean ; Chukchi Sea ; Phytoplankton ; Blooms ; Sea ice ; Snow depth ; Light availability ; Nutrient availability
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 6
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    Detection and quantification of oil under sea ice : the view from below (2015)
    Elsevier
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Cold Regions Science and Technology 109 (2015): 9-17, doi:10.1016/j.coldregions.2014.08.004.
    Description: Traditional measures for detecting oil spills in the open-ocean are both difficult to apply and less effective in ice-covered seas. In view of the increasing levels of commercial activity in the Arctic, there is a growing gap between the potential need to respond to an oil spill in Arctic ice-covered waters and the capability to do so. In particular, there is no robust operational capability to remotely locate oil spilt under or encapsulated within sea ice. To date, most research approaches the problem from on or above the sea ice, and thus they suffer from the need to ‘see’ through the ice and overlying snow. Here we present results from a large-scale tank experiment which demonstrate the detection of oil beneath sea ice, and the quantification of the oil layer thickness is achievable through the combined use of an upward-looking camera and sonar deployed in the water column below a covering of sea ice. This approach using acoustic and visible measurements from below is simple and effective, and potentially transformative with respect to the operational response to oil spills in the Arctic marine environment. These results open up a new direction of research into oil detection in ice-covered seas, as well as describing a new and important role for underwater vehicles as platforms for oil-detecting sensors under Arctic sea ice.
    Description: This work was funded through a competitive grant for the detection of oil under ice obtained from Prince William Sound Oil Spill Recovery Institute (OSRI) (11-10-09). Additional funding/resources was obtained through the EU FP7 funded ACCESS programme (Grant Agreement n°. 265863).
    Keywords: Arctic ; Oil spill ; Sea ice ; Oil detection ; Sonar ; Camera
    Repository Name: Woods Hole Open Access Server
    Type: Article
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