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  • 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk  (6)
  • J strain  (3)
  • Elsevier  (6)
  • Fondazione Eucentre  (2)
  • Copernicus Publications  (1)
  • MDPI Publishing
  • Periodicals Archive Online (PAO)
  • 2015-2019  (9)
  • 1965-1969
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  • 1
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    Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer (2017)
    Elsevier
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 188–193, doi:10.1016/j.ydbio.2016.03.006.
    Description: Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated that TALENs are able to induce mutations in F0 Xenopus embryos, but none has demonstrated germline transmission of such mutations in X. laevis. In this report we used the oocyte host-transfer method to generate mutations in both tyrosinase homeologs and found highly-penetrant germline mutations; in contrast, embryonic injections yielded few germline mutations. We also compared the distribution of mutations in several F0 somatic tissues and germ cells and found that the majority of mutations in each tissue were different. These results establish that X. laevis J strain animals are very useful for generating germline mutations and that the oocyte host-transfer method is an efficient technique for generating mutations in both homeologs.
    Description: This work was supported by grants from the NIH (OD010997 and HD084409).
    Keywords: Xenopus laevis ; TALENs ; J strain ; Tyrosinase ; Oocyte host-transfer ; Genome editing
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 2
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    Luteinizing Hormone is an effective replacement for hCG to induce ovulation in Xenopus (2017)
    Elsevier
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 442–448, doi:10.1016/j.ydbio.2016.05.028.
    Description: Injection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well as lack of a well tested alternative have resulted in frustrating experimental delays in laboratories that predominantly use Xenopus in their research. Mammalian Luteinizing Hormones (LH) share structural similarity, functional equivalency, and bind the same receptor as hCG; this suggests that LH may serve as a good alternative to hCG for promoting ovulation in Xenopus. LH has been found to induce maturation of Xenopus oocytes in vitro, but whether it can be used to induce ovulation in vivo has not been examined. Here we compared the ability of four mammalian LH proteins, bovine (bLH), human (hLH), ovine (oLH), porcine (pLH), to induce ovulation in Xenopus when injected into the dorsal lymph sac of sexually mature females. We find that both ovine and human LH, but not bovine or porcine, are good substitutes for hCG for induction of ovulation in WT and J strain Xenopus laevis and Xenopus tropicalis.
    Description: This work was supported by a grant from the NIHP40OD010997.
    Keywords: Xenopus laevis ; J strain ; Luteinizing Hormone ; Ovulation ; Chorionic gonadotropin
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 3
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    Expanding the genetic toolkit in Xenopus : approaches and opportunities for human disease modeling (2017)
    Elsevier
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 325-335, doi:10.1016/j.ydbio.2016.04.009.
    Description: The amphibian model Xenopus, has been used extensively over the past century to study multiple aspects of cell and developmental biology. Xenopus offers advantages of a non-mammalian system, including high fecundity, external development, and simple housing requirements, with additional advantages of large embryos, highly conserved developmental processes, and close evolutionary relationship to higher vertebrates. There are two main species of Xenopus used in biomedical research, Xenopus laevis and Xenopus tropicalis; the common perception is that both species are excellent models for embryological and cell biological studies, but only Xenopus tropicalis is useful as a genetic model. The recent completion of the Xenopus laevis genome sequence combined with implementation of genome editing tools, such as TALENs (transcription activator-like effector nucleases) and CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated nucleases), greatly facilitates the use of both Xenopus laevis and Xenopus tropicalis for understanding gene function in development and disease. In this paper, we review recent advances made in Xenopus laevis and Xenopus tropicalis with TALENs and CRISPR-Cas and discuss the various approaches that have been used to generate knockout and knock-in animals in both species. These advances show that both Xenopus species are useful for genetic approaches and in particular counters the notion that Xenopus laevis is not amenable to genetic manipulations.
    Description: This work was supported by the National Institutes of Health (P40 OD010997 to M.E.H., R01 HD084409 to M.E.H., R01 HL112618 to P.T. and F.C., and R01 HL127640 to P.T. and F.C.; and the U.S. Environmental Protection Agency (G11E10367 to D.F.).
    Keywords: CRISPR-Cas ; TALENs ; J strain ; Xenopus laevis ; Xenopus tropicalis ; Knock-in ; Human disease model
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 4
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    Uncovering deformation processes from surface displacements (2016)
    Elsevier
    Details
    Publication Date: 2021-05-12
    Description: Today, satellite remote sensing has reached a key role in Earth Sciences. In particular, Synthetic ApertureRadar (SAR) sensors and SAR Interferometry (InSAR) techniques are widely used for the study of dynamicprocesses occurring inside our living planet. Over the past 3 decades, InSAR has been applied for mappingtopography and deformation at the Earth’s surface. These maps are widely used in tectonics, seismology,geomorphology, and volcanology, in order to investigate the kinematics and dynamics of crustal faulting,the causes of postseismic and interseismic displacements, the dynamics of gravity driven slope failures,and the deformation associated with subsurface movement of water, hydrocarbons or magmatic fluids.
    Description: Published
    Description: 58-82
    Description: 1T. Geodinamica e interno della Terra
    Description: 4T. Fisica dei terremoti e scenari cosismici
    Description: 3V. Dinamiche e scenari eruttivi
    Description: JCR Journal
    Description: reserved
    Keywords: SAR ; InSAR ; Earth observation ; Surface displacements ; Satellite missions ; Advanced InSAR ; Earthquake studies ; Volcanic studies ; Tectonic process ; Coseismic studies ; Soil liquefaction ; Post-seismic studies ; Interseismic studies ; Volcanic unrest ; Pre-eruptive phase ; Eruptive phase ; 04. Solid Earth::04.03. Geodesy::04.03.01. Crustal deformations ; 04. Solid Earth::04.03. Geodesy::04.03.06. Measurements and monitoring ; 04. Solid Earth::04.03. Geodesy::04.03.07. Satellite geodesy ; 04. Solid Earth::04.03. Geodesy::04.03.09. Instruments and techniques ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk ; 04. Solid Earth::04.07. Tectonophysics::04.07.07. Tectonics
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 5
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    The multi-sensory approach as a geoeducational strategy (2015)
    Copernicus Publications
    Details
    Publication Date: 2017-04-04
    Description: Geoscience knowledge has a strong impact in modern society as it relates to natural hazards, sustainability and environmental issues. The general public has a demanding attitude towards the understanding of crucial geo-scientific topics that is only partly satisfied by science communication strategies and/or by outreach or school programs. A proper knowledge of the phenomena might help trigger crucial inquiries when approaching mitigation of geohazards and geo-resources, while providing the right tool for the understanding of news and ideas floating from the web or other media, and, in other words, help communication to be more efficient. Nonetheless available educational resources seem to be inadequate in meeting the goal, while research institutions are facing the challenge to experience new communication strategies and non-conventional way of learning capable to allow the understanding of crucial scientific contents. We suggest the use of multi-sensory approach as a successful non-conventional way of learning for children and as a different perspective of learning for older students and adults. Sense organs stimulation are perceived and processed to build the knowledge of the surrounding, including all sorts of hazards. Powerfully relying in the sense of sight, Humans have somehow lost most of their ability for a deep perception of the environment enriched by all the other senses. Since hazards involve emotions we argue that new ways to approach the learning might go exactly through emotions that one might stress with a tactile experience, a hearing or smell stimulation. To test and support our idea we are building a package of learning activities and exhibits based on a multi-sensory experience where the sight is not allowed.
    Description: Unpublished
    Description: Vienna
    Description: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Description: restricted
    Keywords: seismic hazard, volcanic hazard, disasters prevention, education ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Poster session
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  • 6
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    La classificazione sismica in Italia, oggi (2015)
    Fondazione Eucentre
    Details
    Publication Date: 2017-04-04
    Description: In questo articolo si fa il punto sulla situazione della cosiddetta “classificazione sismica” in Italia. Con classificazione sismica si è inteso, fino al 2009, l’associazione dei vari comuni italiani a una categoria, o zona, sismica, e conseguentemente alle relative prescrizioni per le costruzioni, di severità crescente. A partire dal 2000 le competenze in materia di normativa sismica sono state ripartite fra stato e regioni; in particolare la classificazione sismica è stata affidata a queste ultime (la Regione Lombardia ha emanato nel 2014 il proprio decreto ma ne ha rinviato l’attuazione all’ottobre 2015). Nel periodo che va dal 2003 (Ordinanza PCM 3279) al 2009 (entrata in vigore delle NTC08) le classificazioni sismiche regionali sono state modificate varie volte e a tali modifiche si sono accompagnate deroghe di varie natura. Dal 2009 le NTC08 determinano l’azione sismica di riferimento in un modo diverso da prima, passando da quattro zone, e quindi quattro spettri di progetto, per tutto il territorio a valori puntuali. Di conseguenza la classificazione sismica ha mantenuto soltanto il compito di indirizzare controlli sui progetti e di determinare priorità di intervento. Per questi obiettivi si possono ipotizzare per il futuro indicatori diversi da quelli attuali
    Description: Published
    Description: 13-23
    Description: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Description: N/A or not JCR
    Description: restricted
    Keywords: Classificazione sismica ; Norme sismiche ; spettri di progetto ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 7
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    Modelli di pericolosità sismica per l’area italiana: “MPS04-S1” e “SHARE” (2015)
    Fondazione Eucentre
    Details
    Publication Date: 2017-04-04
    Description: Nel 2013 il progetto CE “SHARE” (Seismic Hazard Harmonization in Europe; http://www.shareeu. org/) ha prodotto un modello di pericolosità sismica per l’area europea, i cui risultati sono tuttora in via di distribuzione. Questi risultati rappresentano di fatto la prima valutazione di pericolosità sismica completa per il territorio italiano a partire dal 2006, anno in cui è stato rilasciato il modello di pericolosità di riferimento nazionale (MPS04-S1, http://esse1-gis.mi.ingv.it/). In questo lavoro si propongono alcune analisi delle differenze fra i due modelli, che mostrano come ad alcune differenze nei valori di PGA (10% di probabilità di eccedenza in 50 anni) si accompagnino differenze, anche di segno opposto, negli spettri in accelerazione a pericolosità uniforme. Le analisi mostrano anche che i tassi di sismicità di SHARE sono pressochè simili a quelli di MPS04-S1 e che le ragioni delle differenze più significative sembrano da ricercare nei diversi modelli di attenuazione del moto sismico utilizzati nei due studi.
    Description: Published
    Description: 15-25
    Description: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Description: N/A or not JCR
    Description: restricted
    Keywords: Pericolosità sismica ; Normativa sismica ; Spettri a pericolosità uniforme ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 8
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    Geoethics: ethical challenges and case studies in Earth Science (2015)
    Elsevier
    Details
    Publication Date: 2017-04-04
    Description: Key Features. Written by a global group of contributors with backgrounds ranging from philosopher to geo-practitioner, providing a balance of voices. Includes case studies, showing where experts have gone wrong and where key organizations have ignored facts, wanting assessments favorable to their agendas. Provides a much needed basis for discussion to guide scientists to consider their responsibilities and to improve communication with the public. Description. Edited by two experts in the area, Geoethics: Ethical Challenges and Case Studies in Earth Sciences addresses a range of topics surrounding the concept of ethics in geoscience, making it an important reference for any Earth scientist with a growing concern for sustainable development and social responsibility. This book will provide the reader with some obvious and some hidden information you need for understanding where experts have not served the public, what more could have been done to reach and serve the public and the ethical issues surrounding the Earth Sciences, from a global perspective. Table of contents. Section 1: Introduction Section 2: Philosophical reflections Section 3: The ethics of practice Section 4: Man made hazards Section 5: Natural hazards Section 6: Exploitation of resources Section 7: Low income and indigenous communities Section 8: Geoscience community
    Description: Published
    Description: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Description: 5T. Sorveglianza sismica e operatività post-terremoto
    Description: 4V. Vulcani e ambiente
    Description: 5V. Sorveglianza vulcanica ed emergenze
    Description: 4A. Clima e Oceani
    Description: 6A. Monitoraggio ambientale, sicurezza e territorio
    Description: open
    Keywords: Geoethics ; Philosophy ; Natural hazards ; Man made hazards ; Georesources ; Low income countries ; Geoscience community ; Communication ; Geoeducation ; Natural risks ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk ; 04. Solid Earth::04.08. Volcanology::04.08.08. Volcanic risk ; 05. General::05.03. Educational, History of Science, Public Issues::05.03.99. General or miscellaneous ; 05. General::05.08. Risk::05.08.99. General or miscellaneous ; 05. General::05.09. Miscellaneous::05.09.99. General or miscellaneous
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: book
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  • 9
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    Seismic damage to pipelines in the framework of Na-Tech risk assessment (2015)
    Elsevier
    Details
    Publication Date: 2020-05-29
    Description: The structural integrity of pipelines undergone seismic waves is crucial for industrial installation and for the distributed transportation networks of gaseous and liquid fluids. However, it is nowadays proved that the definition of seismic vulnerability based on purely, structural-derived limit states or on return-to-service or even on the purely economic repair rate indications, is not sufficient for the holistic analysis of risks. On the other hand, detailed numerical studies based on full analyses (including fluid/soil/structure interaction) are too expensive for the aims of risk assessment and simplified methodologies are still needed.In this paper, a large database of earthquake-induced damage for steel and non-steel pipelines is presented. Each case was analyzed and collected from post-earthquake reconnaissance, seismic engineering reports and technical papers. The database may be adopted for the definition of specific vulnerability function (fragility curves), which are commonly implemented in multi-hazard analyses, and more in general for the assessment of Na-Tech risks (Natural events triggering Technological disasters). Seismic damage to pipelines in the framework of Na-Tech risk assessment. Available from: https://www.researchgate.net/publication/271673585_Seismic_damage_to_pipelines_in_the_framework_of_Na-Tech_risk_assessment [accessed Jun 12, 2015].
    Description: Published
    Description: 159-162
    Description: 3T. Pericolosità sismica e contributo alla definizione del rischio
    Description: JCR Journal
    Description: restricted
    Keywords: Earthquake ; Na-Tech ; Ground failure ; Lifeline ; Fragility curve ; Pipelines ; 04. Solid Earth::04.06. Seismology::04.06.11. Seismic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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