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  • Biogeochemistry  (7)
  • American Geophysical Union  (3)
  • Frontiers Media  (3)
  • Cambridge University Press  (1)
  • American Institute of Physics (AIP)
  • Periodicals Archive Online (PAO)
  • 2020-2023  (7)
  • 1935-1939
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  • 1
    Publication Date: 2022-10-26
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Priscu, J. C., Kalin, J., Winans, J., Campbell, T., Siegfried, M. R., Skidmore, M., Dore, J. E., Leventer, A., Harwood, D. M., Duling, D., Zook, R., Burnett, J., Gibson, D., Krula, E., Mironov, A., McManis, J., Roberts, G., Rosenheim, B. E., Christner, B. C., Kasic, K., Fricker, H. A., Lyons, W. B., Barker, J., Bowling, M., Collins, B., Davis, C., Gagnon, A., Gardner, C., Gustafson, C., Kim, O-S., Li, W., Michaud, A., Patterson, M. O., Tranter, M., Ryan Venturelli, R., Trista Vick-Majors, T., & Elsworth, C. Scientific access into Mercer Subglacial Lake: scientific objectives, drilling operations and initial observations. Annals of Glaciology, 62(85–86), (2021): 340–352, https://doi.org/10.1017/aog.2021.10.
    Description: The Subglacial Antarctic Lakes Scientific Access (SALSA) Project accessed Mercer Subglacial Lake using environmentally clean hot-water drilling to examine interactions among ice, water, sediment, rock, microbes and carbon reservoirs within the lake water column and underlying sediments. A ~0.4 m diameter borehole was melted through 1087 m of ice and maintained over ~10 days, allowing observation of ice properties and collection of water and sediment with various tools. Over this period, SALSA collected: 60 L of lake water and 10 L of deep borehole water; microbes 〉0.2 μm in diameter from in situ filtration of ~100 L of lake water; 10 multicores 0.32–0.49 m long; 1.0 and 1.76 m long gravity cores; three conductivity–temperature–depth profiles of borehole and lake water; five discrete depth current meter measurements in the lake and images of ice, the lake water–ice interface and lake sediments. Temperature and conductivity data showed the hydrodynamic character of water mixing between the borehole and lake after entry. Models simulating melting of the ~6 m thick basal accreted ice layer imply that debris fall-out through the ~15 m water column to the lake sediments from borehole melting had little effect on the stratigraphy of surficial sediment cores.
    Description: This material is based upon work supported by the US National Science Foundation, Section for Antarctic Sciences, Antarctic Integrated System Science program as part of the interdisciplinary (Subglacial Antarctic Lakes Scientific Access (SALSA): Integrated study of carbon cycling in hydrologically-active subglacial environments) project (NSF-OPP 1543537, 1543396, 1543405, 1543453 and 1543441). Ok-Sun Kim was funded by the Korean Polar Research Institute. We are particularly thankful to the SALSA traverse personnel for crucial technical and logistical support. The United States Antarctic Program enabled our fieldwork; the New York Air National Guard and Kenn Borek Air provided air support; UNAVCO provided geodetic instrument support. Hot water drilling activities, including repair and upgrade modifications of the WISSARD hot water drill system, for the SALSA project were supported by a subaward from the Ice Drilling Program of Dartmouth College (NSF-PLR 1327315) to the University of Nebraska-Lincoln. J. Lawrence assisted with manuscript preparation. Finally, we are grateful to C. Dean, the SALSA Project Manager, and R. Ricards, SALSA Project Coordinator at McMurdo Station, for their organizational skills, and B. Huber of Lamont-Doherty Earth Observatory for providing the SBE39 PT sensors and the Nortek Aquadopp current meter and assisting with interpretation of the data. B. Huber also provided helpful input on programing and calibrating the SBE19PlusV2 6112 CTD.
    Keywords: Antarctic glaciology ; Basal ice ; Biogeochemistry ; Glacial sedimentology ; Subglacial lakes
    Repository Name: Woods Hole Open Access Server
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  • 2
    Publication Date: 2022-10-26
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Cavaco, M. A., Bhatia, M. P., Hawley, A. K., Torres-Beltran, M., Johnson, W. M., Longnecker, K., Konwar, K., Kujawinski, E. B., & Hallam, S. J. Pathway-centric analysis of microbial metabolic potential and expression along nutrient and energy gradients in the western Atlantic Ocean. Frontiers in Marine Science, 9, (2022): 867310, https://doi.org/10.3389/fmars.2022.867310.
    Description: Microbial communities play integral roles in driving nutrient and energy transformations in the ocean, collectively contributing to fundamental biogeochemical cycles. Although it is well known that these communities are stratified within the water column, there remains limited knowledge of how metabolic pathways are distributed and expressed. Here, we investigate pathway distribution and expression patterns from surface (5 m) to deep dark ocean (4000 m) at three stations along a 2765 km transect in the western South Atlantic Ocean. This study is based on new data, consisting of 43 samples for 16S rRNA gene sequencing, 20 samples for metagenomics and 19 samples for metatranscriptomics. Consistent with previous observations, we observed vertical zonation of microbial community structure largely partitioned between light and dark ocean waters. The metabolic pathways inferred from genomic sequence information and gene expression stratified with depth. For example, expression of photosynthetic pathways increased in sunlit waters. Conversely, expression of pathways related to carbon conversion processes, particularly those involving recalcitrant and organic carbon degradation pathways (i.e., oxidation of formaldehyde) increased in dark ocean waters. We also observed correlations between indicator taxa for specific depths with the selective expression of metabolic pathways. For example, SAR202, prevalent in deep waters, was strongly correlated with expression of the methanol oxidation pathway. From a biogeographic perspective, microbial communities along the transect encoded similar metabolic potential with some latitudinal stratification in gene expression. For example, at a station influenced by input from the Amazon River, expression of pathways related to oxidative stress was increased. Finally, when pairing distinct correlations between specific particulate metabolites (e.g., DMSP, AMP and MTA) and both the taxonomic microbial community and metatranscriptomic pathways across depth and space, we were able to observe how changes in the marine metabolite pool may be influenced by microbial function and vice versa. Taken together, these results indicate that marine microbial communities encode a core repertoire of widely distributed metabolic pathways that are differentially regulated along nutrient and energy gradients. Such pathway distribution patterns are consistent with robustness in microbial food webs and indicate a high degree of functional redundancy.
    Description: This work was funded by the NSF Division of Ocean Sciences (Grant no. OCE-1154320 to EK and KL) and a small (“Microbial controls on marine organic carbon cycling”) and large (“Marine microbial communities from the Southern Atlantic Ocean transect to study dissolved organic matter and carbon cycling”) community sequencing grants from the Joint Genome Institute (US Department of Energy, Walnut Creek, CA) to SH and MB. MB was supported by an NSERC post-doctoral fellowship and a CIFAR Global Scholars fellowship. MC was supported by a Campus Alberta Innovates Program (CAIP) chair to MB.
    Keywords: Marine microbiology ; Metagenomics ; Metatranscriptomics ; Metabolites ; Atlantic Ocean ; Biogeochemistry ; Metabolic pathways ; Functional redundancy
    Repository Name: Woods Hole Open Access Server
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  • 3
    Publication Date: 2022-05-27
    Description: Author Posting. © American Geophysical Union, 2021. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 126(12),(2021): e2021JC017884, https://doi.org/10.1029/2021JC017884.
    Description: The Southern Ocean, an important region for the uptake of anthropogenic carbon dioxide (CO2), features strong surface currents due to substantial mesoscale meanders and eddies. These features interact with the wind and modify the momentum transfer from the atmosphere to the ocean. Although such interactions are known to reduce momentum transfer, their impact on air-sea carbon exchange remains unclear. Using a 1/20° physical-biogeochemical coupled ocean model, we examined the impact of the current-wind interaction on the surface carbon concentration and the air-sea carbon exchange in the Southern Ocean. The current-wind interaction decreased winter partial pressure of CO2 (pCO2) at the ocean surface mainly south of the northern subantarctic front. It also reduced pCO2 in summer, indicating enhanced uptake, but not to the same extent as the winter loss. Consequently, the net outgassing of CO2 was found to be reduced by approximately 17% when including current-wind interaction. These changes stem from the combined effect of vertical mixing and Ekman divergence. A budget analysis of dissolved inorganic carbon (DIC) revealed that a weakening of vertical mixing by current-wind interaction reduces the carbon supply from below, and particularly so in winter. The weaker wind stress additionally lowers the subsurface DIC concentration in summer, which can affect the vertical diffusive flux of carbon in winter. Our study suggests that ignoring current-wind interactions in the Southern Ocean can overestimate winter CO2 outgassing.
    Description: The Southern Ocean, an important region for the uptake of anthropogenic carbon dioxide (CO2), features strong surface currents due to substantial mesoscale meanders and eddies. These features interact with the wind and modify the momentum transfer from the atmosphere to the ocean. Although such interactions are known to reduce momentum transfer, their impact on air-sea carbon exchange remains unclear. Using a 1/20° physical-biogeochemical coupled ocean model, we examined the impact of the current-wind interaction on the surface carbon concentration and the air-sea carbon exchange in the Southern Ocean. The current-wind interaction decreased winter partial pressure of CO2 (pCO2) at the ocean surface mainly south of the northern subantarctic front. It also reduced pCO2 in summer, indicating enhanced uptake, but not to the same extent as the winter loss. Consequently, the net outgassing of CO2 was found to be reduced by approximately 17% when including current-wind interaction. These changes stem from the combined effect of vertical mixing and Ekman divergence. A budget analysis of dissolved inorganic carbon (DIC) revealed that a weakening of vertical mixing by current-wind interaction reduces the carbon supply from below, and particularly so in winter. The weaker wind stress additionally lowers the subsurface DIC concentration in summer, which can affect the vertical diffusive flux of carbon in winter. Our study suggests that ignoring current-wind interactions in the Southern Ocean can overestimate winter CO2 outgassing.
    Description: 2022-05-15
    Keywords: Southern Ocean ; Current-Wind interaction ; CO2 flux ; Air-Sea interaction ; Biogeochemistry ; DIC
    Repository Name: Woods Hole Open Access Server
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  • 4
    Publication Date: 2022-12-22
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Subhas, A., Marx, L., Reynolds, S., Flohr, A., Mawji, E., Brown, P., & Cael, B. Microbial ecosystem responses to alkalinity enhancement in the North Atlantic Subtropical Gyre. Frontiers in Climate, 4, (2022): 784997, https://doi.org/10.3389./fclim.2022.784997
    Description: In addition to reducing carbon dioxide (CO2) emissions, actively removing CO2 from the atmosphere is widely considered necessary to keep global warming well below 2°C. Ocean Alkalinity Enhancement (OAE) describes a suite of such CO2 removal processes that all involve enhancing the buffering capacity of seawater. In theory, OAE both stores carbon and offsets ocean acidification. In practice, the response of the marine biogeochemical system to OAE must be demonstrably negligible, or at least manageable, before it can be deployed at scale. We tested the OAE response of two natural seawater mixed layer microbial communities in the North Atlantic Subtropical Gyre, one at the Western gyre boundary, and one in the middle of the gyre. We conducted 4-day microcosm incubation experiments at sea, spiked with three increasing amounts of alkaline sodium salts and a 13C-bicarbonate tracer at constant pCO2. We then measured a suite of dissolved and particulate parameters to constrain the chemical and biological response to these additions. Microbial communities demonstrated occasionally measurable, but mostly negligible, responses to alkalinity enhancement. Neither site showed a significant increase in biologically produced CaCO3, even at extreme alkalinity loadings of +2,000 μmol kg−1. At the gyre boundary, alkalinity enhancement did not significantly impact net primary production rates. In contrast, net primary production in the central gyre decreased by ~30% in response to alkalinity enhancement. The central gyre incubations demonstrated a shift toward smaller particle size classes, suggesting that OAE may impact community composition and/or aggregation/disaggregation processes. In terms of chemical effects, we identify equilibration of seawater pCO2, inorganic CaCO3 precipitation, and immediate effects during mixing of alkaline solutions with seawater, as important considerations for developing experimental OAE methodologies, and for practical OAE deployment. These initial results underscore the importance of performing more studies of OAE in diverse marine environments, and the need to investigate the coupling between OAE, inorganic processes, and microbial community composition.
    Description: AS was supported through WHOI internal and Assistant Scientist Startup funding. LM and SR were supported by the University of Portsmouth Ph.D. scheme and the UK NERC National Capability programme CLASS (Climate Linked Atlantic Sector Science) ECR Fellowship. BC, AF, EM, and PB were supported by the UK NERC National Capability programme CLASS, grant number NE/R015953/1.
    Keywords: Climate—change ; Ocean alkalinity enhancement ; Biogeochemistry ; North Atlantic ; Carbon flux
    Repository Name: Woods Hole Open Access Server
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  • 5
    Publication Date: 2022-10-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Maas, A. E., Liu, S., Bolanos, L. M., Widner, B., Parsons, R., Kujawinski, E. B., Blanco-Bercial, L., & Carlson, C. A. Migratory zooplankton excreta and its influence on prokaryotic communities. Frontiers in Marine Science, 7, (2020): 573268, doi:10.3389/fmars.2020.573268.
    Description: Particulate organic matter (POM) (fecal pellets) from zooplankton has been demonstrated to be an important nutrient source for the pelagic prokaryotic community. Significantly less is known about the chemical composition of the dissolved organic matter (DOM) produced by these eukaryotes and its influence on pelagic ecosystem structure. Zooplankton migrators, which daily transport surface-derived compounds to depth, may act as important vectors of limiting nutrients for mesopelagic microbial communities. In this role, zooplankton may increase the DOM remineralization rate by heterotrophic prokaryotes through the creation of nutrient rich “hot spots” that could potentially increase niche diversity. To explore these interactions, we collected the migratory copepod Pleuromamma xiphias from the northwestern Sargasso Sea and sampled its excreta after 12–16 h of incubation. We measured bulk dissolved organic carbon (DOC), dissolved free amino acids (DFAA) via high performance liquid chromatography and dissolved targeted metabolites via quantitative mass spectrometry (UPLC-ESI-MSMS) to quantify organic zooplankton excreta production and characterize its composition. We observed production of labile DOM, including amino acids, vitamins, and nucleosides. Additionally, we harvested a portion of the excreta and subsequently used it as the growth medium for mesopelagic (200 m) bacterioplankton dilution cultures. In zooplankton excreta treatments we observed a four-fold increase in bacterioplankton cell densities that reached stationary growth phase after five days of dark incubation. Analyses of 16S rRNA gene amplicons suggested a shift from oligotrophs typical of open ocean and mesopelagic prokaryotic communities to more copiotrophic bacterial lineages in the presence of zooplankton excreta. These results support the hypothesis that zooplankton and prokaryotes are engaged in complex and indirect ecological interactions, broadening our understanding of the microbial loop.
    Description: Funding for this research was provided by Simons Foundation International as part of the BIOS-SCOPE project to AM, LB-B, CC, and EK.
    Keywords: DOC ; Dissolved metabolites ; Diel vertical migration ; Biogeochemistry ; Copepod
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  • 6
    Publication Date: 2022-10-20
    Description: Author Posting. © American Geophysical Union, 2020. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 47 (2020): e2020GL087669, doi:10.1029/2020GL087669.
    Description: We present a year‐round time series of dissolved methane (CH4), along with targeted observations during ice melt of CH4 and carbon dioxide (CO2) in a river and estuary adjacent to Cambridge Bay, Nunavut, Canada. During the freshet, CH4 concentrations in the river and ice‐covered estuary were up to 240,000% saturation and 19,000% saturation, respectively, but quickly dropped by 〉100‐fold following ice melt. Observations with a robotic kayak revealed that river‐derived CH4 and CO2 were transported to the estuary and rapidly ventilated to the atmosphere once ice cover retreated. We estimate that river discharge accounts for 〉95% of annual CH4 sea‐to‐air emissions from the estuary. These results demonstrate the importance of resolving seasonal dynamics in order to estimate greenhouse gas emissions from polar systems.
    Description: All data generated by the authors that were used in this article are available on PANGAEA (https://doi.org/10.1594/PANGAEA.907159) and model code for estimating CH4 transport is available on GitHub (https://doi.org/10.5281/zenodo.3785893). We acknowledge the use of imagery from the NASA Worldview application (https://worldview.earthdata.nasa.gov), part of the NASA Earth Observing System Data and Information System (EOSDIS), and data from Ocean Networks Canada, and Environment Canada. We thank everyone involved in the fieldwork including C. Amegainik, Y. Bernard, A. Cranch, F. Emingak, S. Marriott, and A. Pedersen. Laboratory analysis and experiments were performed by A. Cranch, R. McCulloch, A. Morrison, and Z. Zheng. We thank J. Brinckerhoff, the Arctic Research Foundation, and the staff of the Canadian High Arctic Research Station for support with field logistics. Funding for the work was provided by MEOPAR NCE funding to B. Else, a WHOI Interdisciplinary Award to A. Michel., D. Nicholson. and S. Wankel, and Canadian NSERC grants to P. Tortell. and B. Else. Authors received fellowships, scholarships, and travel grants including an NSERC postdoctoral fellowship to C. Manning, an NDSEG fellowship to V. Preston, NSERC PGS‐D and Izaak Walton Killam Pre‐Doctoral scholarships to S. Jones, and Northern Scientific Training Program funds (Polar Knowledge Canada, administered by the Arctic Institute of North America, University of Calgary) to S. Jones and P. Duke. We also thank Polar Knowledge Canada (POLAR) and Nunavut Arctic College for laboratory space and field logistics support.
    Description: 2020-10-23
    Keywords: Greenhouse gases ; Biogeochemistry ; Arctic coastal waters ; Biogeochemical sensing ; Seasonal cycles ; Methane
    Repository Name: Woods Hole Open Access Server
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  • 7
    Publication Date: 2022-10-26
    Description: Author Posting. © American Geophysical Union, 2020. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Biogeosciences 125(10), (2020): e2020JG005664, doi:10.1029/2020JG005664.
    Description: Shallow ponds are expanding in many salt marshes with potential impacts on ecosystem functioning. Determining how pond characteristics change over time and scale with physical dimensions and other spatial predictors could facilitate incorporation of ponds into projections of ecosystem change. We evaluated scaling relationships across six differently sized ponds in three regions of the high marshes within the Plum Island Ecosystems‐Long Term Ecological Research site (MA, USA). We further characterized diel fluctuations in surface water chemistry in two ponds to understand short‐term processes that affect emergent properties (e.g., habitat suitability). Primary producers drove oxygen levels to supersaturation during the day, while nighttime respiration resulted in hypoxic to anoxic conditions. Diel swings in oxygen were mirrored by pH and resulted in successive shifts in redox‐sensitive metabolisms, as indicated by nitrate consumption at dusk followed by peaks in ammonium and then sulfide overnight. Abundances of macroalgae and Ruppia maritima correlated with whole‐pond oxygen metabolism rates, but not with surface area (SA), volume (V), or SA:V. Moreover, there were no clear patterns in primary producer abundances, surface water chemistry, or pond metabolism rates across marsh regions supplied by different tidal creeks or that differed in distance to upland borders or creekbanks. Comparisons with data from 2 years prior demonstrate that plant communities and biogeochemical processes are not in steady state. Factors contributing to variability between ponds and years are unclear but likely include infrequent tidal exchange. Temporal and spatial variability and the absence of scaling relationships complicate the integration of high marsh ponds into ecosystem biogeochemical models.
    Description: Thanks to S. McNichol, S. Jayne, E. Neel, and PIE‐LTER (NSF‐OCE1238212) for field assistance; I. Forbrich for meteorological data (Giblin & Forbrich, 2018); J. Jennings for dissolved nutrient analyses; J. Seewald for ion chromatograph access; and G. Mariotti for elevation data. C. Wilson and an anonymous reviewer provided comments that greatly improved our manuscript. A. C. S. was supported by NSF (OCE1233678), NOAA (NA14NOS4190145), and Sea Grant (NA14OAR4170104) awards, and A. D. by the MIT Undergraduate Research Opportunities Program.
    Description: 2021-03-15
    Keywords: Salt marsh ; Global change ; Biogeochemistry ; Metabolism ; Scaling ; Ecosystem function
    Repository Name: Woods Hole Open Access Server
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