ALBERT

Beschreibung: Variable displacement vane pumps are used in a variety of applications, most prominently in the context of modern automotive vehicles. Minimal models capturing the main dynamic effects of such pumps have remained scarce. In this contribution, a minimal model taking into account the force effects from line pressure exposure and dead volume compression and expansion is presented. The pump model is autonomous and allows for a simple integration in simulation models for which an example in the form of a non-smooth hydraulic circuit is given. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: In this contribution, the LuGre model is extended and the effect of frictional aging is incorporated using the state variable approach. The adapted friction model is used in combination with a single-degree-of-freedom oscillator to investigate the basic effects on the stick-slip amplitude and period. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: Nano-silver and nano-titanium oxide films can be coated over brackets in order to reduce bacterial aggregation and friction. However, their antimicrobial efficacy, surface roughness, and frictional resistance are not assessed before. Fifty-five stainless-steel brackets were divided into 5 groups of 11 brackets each: uncoated brackets, brackets coated with 60 µm silver, 100 µm silver, 60 µm titanium, and 100 µm titanium. Coating was performed using physical vapor deposition method. For friction test, three brackets from each group were randomly selected and tested. For scanning electron microscopy and atomic-force microscopy assessments, one and one brackets were selected from each group. For antibacterial assessment, six brackets were selected from each group. Of them, three were immediately subjected to direct contact with S. mutans . Colonies were counted 3, 6, 24, and 48 h of contact. The other three were stored in water for 3 months. Then were subjected to a similar direct contact test. Results pertaining to both subgroups were combined. Groups were compared statistically. Mean (SD) friction values of the groups 'control, silver-60, silver-100, titanium-60, and titanium-100' were 0.55 ± 0.14, 0.77 ± 0.08, 0.82 ± 0.11, 1.52 ± 0.24, and 1.57 ± 0.41 N, respectively ( p  =   .0004, Kruskal–Wallis). Titanium frictions were significantly greater than control ( p  〈   .05), but silver groups were not ( p  〉   .05, Dunn). In the uncoated group, colony count increased exponentially within 48 h. The coated groups showed significant reductions in colony count ( p  〈   .05, two-way-repeated-measures ANOVA). In conclusions, all four explained coatings reduce surface roughness and bacterial growth. Nano-titanium films are not suitable for friction reduction. Nano-silver results were not conclusive and need future larger studies.

Beschreibung: Many papers have claimed the attainment of super-resolution, i.e. resolution beyond that achieved classically, by measurement of the profile of a feature in the image. We argue that measurement of the contrast of the image of a dark bar on a bright background does not give a measure of resolution, but of detection sensitivity. The width of a bar that gives an intensity at the center of the bar of 0.735 that in the bright region (the same ratio as in the Rayleigh resolution criterion) is for the coherent case with central illumination. This figure, which compares with for the Abbe resolution limit with central illumination, holds for the classical case, and so is no indication of super-resolution. Theoretical images for two points, two lines, arrays of lines, arrays of bars, and grating objects are compared. These results can be used a reference for experimental results, to determine if super-resolution has indeed been attained. The history of the development of the theory of microscope resolution is outlined. This shows the image of a dark bar on a coherent bright background, for different bar widths. There is no sharp cutoff. There is always a dip in intensity at the center, even for a bar width of, corresponding to 0.05 Airy units.

Beschreibung: On the cover: The cover image, by Yulin Lin et al., is based on the Research Article Assessment of fracture risk in proximal tibia with tumorous bone defects by a finite element method , DOI: 10.1002/jemt.22899 .

Beschreibung: Honey is a valuable food produced by bees from sugary substances that they gather in nature. The transformation the nectar into honey, by bees, is long and complex. Except for honey, where heavy metals are absent or are found only in traces, the bees and their products have always been considered excellent biomarkers of such contaminants. We have assumed that the absence of heavy metals in honey is due to the presence of a detoxification system in the digestive system of bees, which involves metallothioneins, proteins that have a role in the homeostatic control of essential and non-essential metals. We have placed the beehives in three different zones: industrial, urban and rural. Investigations were carried out with ICP-MS method for the detection of heavy metals in the guts of honey bees and honey. The metallothioneins have been identified by Immunohistochemical and Western-blotting analisys. The investigations have shown the presence of heavy metals only in bees guts but not in honey, while the presence of metallothionein has been highlighted only in epithelium of the honey sac, demonstrating the existence of an efficient system of detoxification of heavy metals. In honey bees the detoxification system is primarily based on induction of Metallothioneins Metallothionein-immunopositive cells only in the honey sac.

Beschreibung: Site-specific accumulation of flavonoids in Apocyni Veneti Folium was determined by laser scanning confocal microscope (LSCM) and the localization of catechins also was observed via vanillin -HCl staining under the conventional optical microscope. The contents of five flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured using HPLC method. LSCM observation showed that flavonoids are accumulated in cuticle of epidermal cells and vessel walls, especially in protoplasts and nucleolus of the collenchyma cells and the epidermal cells. Catechins are localized in the palisade parenchyma cells and vessel walls, particularly in the laticifers found in the phloem. On the basis of the difference of the maximal emission wavelength between quercetin and kaempferol derivatives which have fluorescence behavior by appropriate treatment, kaempferol and its derivatives are localized exclusively in the cuticle. Results showed that the content of astragalin in Apocyni Veneti Folium from different parts revealed the decreasing trend, while hyperin and isoquercitrin were higher in June and July analyzed by HPLC. In summary, the site-specific accumulation of flavonoids in Apocyni Veneti Folium can be determined by LSCM and vanillin -HCl staining. The contents of flavonoids in Apocyni Veneti Folium are correlated with harvest times and growth parts. Site-specific accumulation of flavonoids was determined by LSCM. Tissue localization of catechins was observed via vanillin-HCl staining. Dynamic change of flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured.

Beschreibung: Canine mammary gland tumors represent the second most frequent type of neoplasm in dogs, being an important problem within veterinary medical field. Canine mixed mammary tumors are the most common; the use of a transmission electron microscope (TEM) can contribute as a tool in its diagnosis by determining the characteristics of cellular components from numerous neoplasms. The aim of this study was to characterize cytologically canine mammary mixed tumor by the use of the TEM. A biopsy collected from an 11 years old bitch Shih-Tzu and analyzed by histopathology was used for ultrastructural analysis. Specimens obtained were double stained using uranyl acetate and lead citrate prior to observation in the TEM. The protocol established to transmission electron microscopy observation allowed the identification of main cellular characteristics of canine mixed mammary tumors; however, it was not possible a detailed visualization of the organelles due to the preservation of the biopsy in formaldehyde. The protocol established allowed the identification of main cellular characteristics; however, it was not possible a detailed visualization of organelles. The scratches in the sectioned tissue were reduced with speed to 1 mm/s.

Beschreibung: In the current work, irregular morphology of Staphylococcus aureus bacteria has been visualized by phase retrieval employing off-axis electron holography (EH) and 3D reconstruction electron tomography using high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM). Bacteria interacting with gold nanoparticles (AuNP) acquired a shrunken or irregular shape due to air dehydration processing. STEM imaging shows the attachment of AuNP on the surface of cells and suggests an irregular 3D morphology of the specimen. The phase reconstruction demonstrates that off-axis electron holography can reveal with a single hologram the morphology of the specimen and the distribution of the functionalized AuNPs. In addition, EH reduces significantly the acquisition time and the cumulative radiation damage (in three orders of magnitude) over biological samples in comparison with multiple tilted electron expositions intrinsic to electron tomography, as well as the processing time and the reconstruction artifacts that may arise during tomogram reconstruction. Off-axis electron holography and electron tomography have been used to show an irregular shape of S. aureus bacteria. EH reduces acquisition and processing time as well as the radiation damage inherent to the long tilting series for tomography.

Beschreibung: Present scanning electron microscope study, reports healing of excised skin wounds in Cirrhinus mrigala . Healing process of wounds, inflicted on head skin, using biopsy punch was observed at intervals—0 hour (h), 1, 2, 4, 6, 12 h, 1 day (d) 2 and 4 d. Accumulation of mucus in wound region within 1h after infliction of wound has been considered an immediate measure to provide protection to injured skin from microbial invasion and other external environmental hazards. On infliction of wound, mobilization of epithelial cells at wound edge is associated with disturbance of coaptive relationship of epithelial cells till original coaptive stability is reached. At 6–12 h appearance of epidermal ridge in region of contact of migrating fronts is due to piling up of epithelial cells. This is associated with cessation of migration of epithelial cells and their simultaneous continual arrival in the region. Speedy epithelialization of skin wounds in C. mrigala like in other fishes, compared to that of mammals and other higher vertebrates, is possibly facilitated owing to surrounding wet external environment. Microridges in initial stages of wound healing appear fragmented without particular orientation. Further, epithelial cells in epithelium in wound region and in region adjacent to wound elongate. These changes are associated with the stretching of epithelial cells indicating their streaming and migration, toward wound. Presence of superficial neuromasts, smallest functional units of lateral line system, a hydrodynamic sensory system, has been associated with important functional significance in fish. Healing of skin wounds in Cirrhinus mrigala was studied using scanning electron microscopy. Covering of wound by mucus, rapid epithelialization, and changes in pattern of microridges on the surface of epithelial cells during healing are significant.

Beschreibung: For the long-term success of implants, it is necessary to achieve a direct contact between the implant and the subjacent bone. To avoid bacterial penetration that could adversely affect the initial wound healing as well as the long-term behavior of the implants, an early tissue barrier must form that is able to protect the biological peri-implant structures. Given the need of an effective tissue early barrier around dental implants, the present study evaluated, in vitro, the influence of physical and chemical characteristics of two implant abutment surfaces on gingival epithelial cells (OBA-9) adhesion. To this end, titanium (Ti) and zirconia (ZrO 2 ) disk-shaped specimens were used mimicking the abutment components surfaces, while bovine enamel (BE) and glass cover slips (GCS) disks served as positive and negative controls, respectively. Roughness and surface free energy (SFE) of all materials were evaluated previously to cellular adhesion step. In sequence, the effect of each material on cells morphology and viability was analyzed after 1 and 24 hr. The results showed that roughness and SFE had no effect on the cell viability data or on their interaction ( p = .559), independent of a post-contact analysis of 1 or 24 hr. However, cells attachment and spreading increased after 24 hr on Ti and ZrO 2 than BE, corresponding to the highest SFE values. SFE appears to be an important property interfering on the quality of the soft tissue surrounding dental implants. These data can be considered a trigger point for developing new material surfaces. Titanium and zirconia substrates disclosed hydrophobic properties and admitted the highest surface free energy values when in comparison to enamel. Well-attached/spreading cells were observed on implant abutment materials.

Beschreibung: Bioceramics are being used in experimental bone engineering application in association with bone marrow derived mesenchymal stem cells (BM-MSCs) as a new therapeutic tool, but their effects on the ultrastructure of BM-MSCs are yet unknown. In this study we report the morphological features of ovine (o)BM-MSCs cultured with Skelite, a resorbable bioceramic based on silicon stabilized tricalcium phosphate (SiTCP), able to promote the repair of induced bone defect in sheep model. oBM-MSCs were isolated from the iliac crest, cultured until they reached near-confluence and incubated with SiTCP. After 48 hr the monolayers were highly damaged and only few cells adhered to the plastic. Thus, SiTCP was removed, and after washing the cells were cultured until they became confluent. Then, they were trypsinizated and processed for transmission electron microscopy (TEM) and RT-PCR analysis. RT-PCR displayed that oBM-MSCs express typical surface marker for MSCs. TEM revealed the presence of electron-lucent cells and electron-dense cells, both expressing the CD90 surface antigen. The prominent feature of electron-lucent cells was the concentration of cytoplasmic organelles around the nucleus as well as large surface blebs containing glycogen or profiles of endoplasmic reticulum. The dark cells had a multilocular appearance by the presence of peripheral vacuoles. Some dark cells contained endocytic vesicles, lysosomes, and glycogen aggregates. oBM-MSCs showed different types of specialized interconnections. The comparison with ultrastructural features of untreated oBM-MSCs suggests the light and dark cells are two distinct cell types which were differently affected by SiTCP bioceramic. Skelite cultured ovine BM-MSCs display electron-dense and electron-lucent cells which are differently affected by this bioceramic. This suggests that they could play a different role in bioceramic based therapy. Skelite cultured ovine bone marrow mesenchymal stem cells display electron-dense and electron-lucent cells which are differently affected by this bioceramic. This suggests that they could play a different role in bioceramic-based therapy.

Beschreibung: The objective of the present study was to compare cleaning methods for delicate insect specimens for investigations with scanning electron microscopy (SEM). As typical specimens we used aquatic larvae of mosquitoes, springtails, larvae of mayflies and caterpillars because they are very fragile and large parts of their body consist of soft tissue. Additionally their cuticle is very often covered with dirt, soil particles or other materials. Cleaning with ultrasonic sound, as the most common cleaning method used for SEM, will destroy fragile insects. Therefore we tested different procedures to remove the dirt particles. In a first approach we compared cleaning with Potassium hydroxide (KOH), Proteinase K, and Triton X in aquatic larvae of flies, which were available in numbers and kept under the same conditions. As our results showed that the treatment with KOH gives the best results we treated in a second approach springtails, larvae of mayflies and caterpillars only with KOH. The springtails and caterpillars were largely free of particles after treatment with KOH; however, the larvae of mayflies were still covered with remnants of diatoms and precipitates of calcium carbonate of the algae. KOH dissolves organic impurities, on the other hand silicon dioxide and lime crusts are not solved. With this limitation, treatment with KOH is a simple technique for routine use as cleaning method for fragile insect specimens for SEM. Cleaning of delicate insect specimens with KOH, Proteinase K, and Triton X for scanning electron microscopy (SEM) was compared. Treatment with KOH gives the best results and is a simple technique for cleaning fragile insect specimens for SEM.

Beschreibung: This study compared several irrigation protocols and application systems for sealer penetration into dentinal tubules. Single-rooted-human teeth were divided into 5 experimental groups ( n  = 15) and a control group ( n  = 5), according to final irrigation protocols: standard needle irrigation (SNI); Vibringe; Vibringe + NaviTip FX (Vibringe NFX); Endo Spray (ES); and passive-ultrasonic-irrigation (PUI). Following obturation of the root canals, the percentage of the sealer penetration was measured at different depths using stepwise CLSM analysis. The sealer penetration in the experimental groups was significantly higher than the control group at all levels ( p  〈 .05). No significant differences were observed between Vibringe and SNI or Vibringe NFX, ES, and PUI at all depths ( p  〉 .05). The Vibringe NFX, ES, and PUI groups allowed deeper sealer penetration than SNI at 100, 250, and 500 μm levels ( p  〈 .05). The irrigant activation, the needle design, and the application form (syringe or spray) may impact the quality of the seal that is achieved with root canal filling. A stepwise approach using CLSM for quantification of sealer penetration allowed understanding of sealer penetration by irrigation methods. Irrigant activation, needle design, and irrigant application form affect the quality of sealer penetration.

Beschreibung: The purpose of this work is to study the dependence of AFM-data reliability on scanning rate. The three-dimensional (3D) surface topography of the samples with different micro-motifs is investigated. The analysis of surface metrics for estimation of artifacts from inappropriate scanning rate is presented. Fractal analysis was done by cube counting method and evaluation of statistical metrics was carrying out on the basis of AFM-data. Combination of quantitate parameters is also presented in graphs for every measurement. The results indicate that the sensitivity to scanning rate growths with fractal dimension of the sample. This approach allows describing the distortion of the images against scanning rate and could be applied for dependences on the other measurement parameters. The article explains the relevance and comparison of fractal and statistical surface parameters for characterization of data distortion caused by inappropriate choice of scanning rate. Three samples with different topography were studied by AFM at different scanning rate. Effect of scanning rate on the result image and geometrical data of surface is investigated. Image distortion could be defined by surface statistical parameters and data of fractal analysis.

Beschreibung: The present study aimed to evaluate the effects of four endodontic chelating agents, followed by 2.5% sodium hypochlorite (NaOCl), as final irrigation regimens on organic and inorganic components of human root dentin. Sixty mandibular incisors were prepared and randomly divided into six groups ( n  = 10): QMiX, 1% peracetic acid (PA), 17% EDTA, 10% citric acid (CA), 2.5% NaOCl (solution control) and distilled water (DW-negative control). After irrigation with the chelating agents, a final flush was performed with 2.5% NaOCl. The specimens were split longitudinally in halves; one was designated for organic component analysis by polarized light microscopy (PLM) and the other for inorganic structure analysis by scanning electron microscopy (SEM). Scores data obtained in the PLM analysis were submitted to Kruskal-Wallis' test, followed by Dunn's test ( p  〈 .05). SEM findings were presented descriptively. NaOCl and DW groups showed uniformity in the fibrillar network and smear layer obliterating the dentinal tubules, while CA group presented alteration in organic and inorganic components of dentin. EDTA group did not show differences from others in the organic component, but altered the inorganic structure. QMiX and PA groups did not cause a significant morphological alteration in collagen and removed the smear layer without inorganic structure modification. As final irrigation, QMiX and PA solutions, followed by 2.5% NaOCl, showed better behavior than the other chelating agents tested, preserving organic and inorganic components of human root dentin. QMiX and PA groups removed the smear layer without inorganic structure modification. QMiX and PA groups did not cause a significant morphological alteration in collagen.

Beschreibung: Nowadays, infertility is no longer considered as an unsolvable disorder due to progresses in germ cells derived from stem lineage with diverse origins. Technical and ethical challenges push researchers to investigate various tissue sources to approach more efficient gametes. The purpose of the current study is to investigate the efficacy of a combined medium, retinoic acid (RA) together with Bone Morphogenic Protein-4 (BMP4), on differentiation of Bone Marrow Mesenchymal Stem Cells (BMMSCs) and adipose-derived mesenchymal stem cells (ADMSCs) into germ cells. Murine MSCs were obtained from both Bone Marrow (BM) and Adipose Tissue (AT) samples and were analyzed for surface markers to get further verification of their nature. BMMSCs and ADMSCs were induced into osteogenic and adipogenic lineage cells respectively, to examine their multipotency. They were finally differentiated into germ cells using media enriched with BMP4 for 4 days followed by addition of RA for 7 days (11 days in total). Analyzing of differentiation potential of BMMSCs- and ADMSCs were performed via Immunofluorescence, Flowcytometry and Real time-PCR techniques for germ cell-specific markers (Mvh, Dazl, Stra8 and Scp3). Mesenchymal surface markers (CD90 and CD44) were expressed on both BMMSCs and ADMSCs, while endothelial and hematopoietic cell markers (CD31 and CD45) had no expression. Finally, all germ-specific markers were expressed in both BM and AT. Although germ cells differentiated from ADMSCs showed faster growth and proliferation as well as easy collection, they significantly expressed germ-specific markers lower than BMMSCs. This suggests stronger differentiation potential of murine BMMSCs than ADMSCs. BMMSCs and ADMSCs could differentiate in appropriate mediums to osteoblast and lipoblast respectively and could express superficial mesenchymal characteristics. ADMSCs and BMMSCs were capable of differentiation into germ cells, and BMMSCs have stronger differentiation potential than ADMSCs.

Beschreibung: The famous lacquer wares excavated from the Jiuliandun Tombs of the middle and late Warring States period (476–221 BC) were analyzed using scanning electron microscopy/energy as well as dispersive X-ray spectrometry (SEM/EDS), Raman spectroscopy (RS), optical microscopy (OM), and X-ray diffraction (XRD). The results showed a multilayer structure in the lacquer film, including a Qihui layer (a layer made of lacquer and various plasters), undercoat layer (or finishing coat) and colored paint layer mixed with various inorganic particles, such as quartz (SiO 2 ) and hydroxyapatite [Ca 5 (PO 4 ) 3 (OH)], as fillers in the Qihui layers or orpiment (As 2 S 3 ) and cinnabar (HgS), which were used as a yellow or red pigment, respectively. With the help of elemental mapping images, a double-layer structure of the lacquer plaster was observed, corresponding to a mixture of lacquer liquid and bone ash [Ca 5 (PO 4 ) 3 (OH)], with large-diameter particles in the ground lacquer layer near the wooden body and small quartz (SiO 2 ) particles in upper lacquer layer. Specifically, quartz particles detected in the undercoat layer as fillers could be beneficial for improving the moshardness value, cost reduction and abrasive resistance of the lacquer film. In fact, the mixed method that used urushi and inorganic particles to form lacquer plaster was an important technological innovation and deeply influenced lacquering technologies worldwide. The results of this study will not only contribute to understanding the importance of lacquer skills in the Chinese Warring States but also provide information for cultural relic conservation as well as modern lacquer manufacturing for their protection and duplication. A double-layer structure of lacquer plaster observed by elemental mapping Large hydroxyapatite particles were found in ground lacquer and small quartz particles were found in the upper lacquer layer. Quartz particles were used in the undercoat layer as fillers to improve the quality of the lacquer film. Plaster made of urushi and inorganic particles was an important ancient technological innovation.

Beschreibung: Radiation beam interface contrast X-ray microscopy provides resolution of a few dozen nanometers from fixed whole muscle biopsies, allowing better reconstruction of the microstructure of the muscle than is currently possible with classic histological techniques. Fixed soleus muscle biopsies have been evaluated from the walk-in mouse model using phase-contrast X-ray microscopy, and results presented that corroborate the accuracy of the method used, and its potential for application in physiotherapy and occupational therapy studies. We believe that this method will enhance existing morphometric methods of analysis, leading to accurate reconstruction of other thick specimens that would otherwise require thin sectioning and reconstruction through deconvolution algorithms. A new method is suggested that can observe the soleus muscle by means of a radiation beam. This study suggests a remarkable method for analyzing muscle tissue that provides a simple and unified technique for obtaining stereoscopic tissue images.

Beschreibung: A bio-micromanipulation system is designed for manipulating micro-objects with a length scale of tens or hundreds of microns based on stereo light microscope. The world coordinate reconstruction of points on the surface of micro-objects is an important goal for the micromanipulation. Traditional pinhole camera model is applied widely in macrocomputer vision. However, this model will output bad data with remarkable error if it is directly used to reconstruct three-dimensional world coordinates for stereo light microscope. Therefore, a novel and improved pinhole camera model applied in bio-micromanipulation system is proposed in this article. The new model is composed of binocular-pinhole model and error-correction model. The binocular-pinhole model is used to output the basic world coordinates. The error-correction model is used to correct the errors from the basic world coordinates and outputs the final high-precision world coordinates. The results show that the new model achieves a precision of 0.01 mm in the X direction, 0.01 mm in the Y direction, and 0.015 mm in the Z direction within a maximum reconstruction distance of 4.1 mm in the X direction, 2.9 mm in the Y direction, and 2.25 mm in the Z direction, and that traditional pinhole camera model achieves a lower and unsatisfactory precision of about 0.1 mm. A stereovision model for stereo light microscope is proposed, which supplies a bio-micromanipulation system with the high-precision world coordinates. Binocular-pinhole model is obtained based on traditional pinhole camera model. Error-correction model corrects the errors from binocular-pinhole model.

Beschreibung: The egg capsules of five systellognathan stoneflies species representing the genus Isoperla (Plecoptera, Perlodidae) have been investigated using light and electron microscopes (SEM and TEM). We consider the structural modifications of egg coverings (egg capsules, eggshells) like: a shape of a capsule, presence of structures fixing an egg to the substratum under water, intrachorionic aeropylar system facilitating gas exchange as a factor adapting an egg/embryo to the environment. The structures protecting eggs against desiccation during a female flight before oviposition into water as well as against the dangers of external mechanical injury caused by turbulences in rapid water currents of mountain streams are described and discussed. The ground plan of the egg capsule in arctoperlarian stoneflies is also discussed. The egg capsules of five Isoperla species were investigated The egg capsules of Isoperla are radially and regionally differentiated The general organization of the egg capsules in all species investigated is similar

Beschreibung: The present study investigated the regenerative potential of connective tissues harvested from two palatal areas widely used as donor sites for muco-gingival surgical approaches. Connective tissue grafts (CTGs) were obtained by de-epithelialisation of a free gingival graft (deCTG) and by a split flap approach from a previous donor site (reCTG). Two types of mesenchymal stem cell (MSCs) were isolated and were named de-epithelialised MSCs (deMSCs) and re-entry MSCs (reMSCs). The cells were characterised and cellular functionality was investigated. CTGs were evaluated using immunohistochemical and ultrastructural approaches. No significant differences were observed regarding the frequency of colony-forming unit- fibroblasts, migration potential, and population doubling time between the two cell lines ( p  〉 0.05). Both cell lines showed positivity for CD105, CD73, CD90, and CD44 and negative expression for CD34/45, CD14, CD79a, and HLA-DR. MSCs from both cell lines successfully differentiated into osteogenic, adipogenic, and chondrogenic lineages. Cells expressing antigens characteristic of CD34+ stromal cells (CD34+, αSMA−, CD31−) were traced in both CTGs. Ultrastructural analysis highlighted the presence of putative progenitors, namely fibroblasts,—in the pericapillary regions and in remote regions of the lamina propria- and pericytes—surrounding the capillaries. This study provides supplementary arguments for the use of CTG grafts in clinical practice due to the presence of putative progenitor cell. However, results were inconclusive regarding clinical decision-making to determine optimal harvesting area. Prior harvesting in the donor area did not appear to alter the regenerative capabilities of the connective tissue. Palatal connective tissues harvested by two techniques foster mesenchymal stem cells. Previous harvesting from the palate does not alter the regenerative capabilities of the connective tissue.

Beschreibung: The chilli thrips, Scirtothrips dorsalis Hood, is a serious pest of numerous important vegetable and ornamental crops. Various signals, especially phytochemical cues, determine the behavior of the phytophagous thrips at host selection. The sensory abilities of S. dorsalis are poorly understood although the antennae of adult are known to possess important sensory structures in orther insects. In this study, the morphology, distribution, and ultrastructure of the antennal sensilla of the S. dorsalis were examined by using scanning and transmission electron microscopy. Microscopy observations revealed that adult male and female S. dorsalis possess filiform antennae. Each antenna comprises a scape, a pedicel, and a flagellum composed of six segments without clear sexual dimorphism in the number and distribution of antennal sensilla. The scape and pedicel exhibit Böhm's bristles, sensilla chaetica, and sensilla campaniform. The external structures of these organs reveal their mechanosensory function. In the flagellum, the most represented sensilla are the multiporous sensilla basiconica, which can be divided into three types of single-walled olfactory sensilla; three types of sensilla chaetica with mechanosensory and gustatory functions; sensilla coeloconica, which possess hollow cuticular spoke channels and represent double-walled olfactory sensilla; sensilla capitula and sensilla cavity with thermo-hygrosensory functions; and aporous sensilla trichodea with smooth cuticula and mechanosensory function. The putative function of described sensilla is discussed in ralation to host plant selection behavior of S. dorsalis . TEM image of Sensilla chaetica type 3 of Scirtothrips dorsalis female antenna. The morphology, distribution, and ultrastructure of the antennal sensilla of the Scirtothrips dorsalis were examined by using scanning and transmission electron microscopy. We examind the antennal sensilla of S. dorsalis at ultrastructural level. The antennae appear rich in sensilla of different kinds and functions. This establish the theoretical foundation to characterize the host selection behavior of thrips.

Beschreibung: This study aimed to prepare silk fibroin (SF) films loaded with honeysuckle flowers extract (HFE) for inducing apoptosis of HeLa cells. We mixed solution of SF and HFE by air-drying for preparing the honeysuckle flowers extract loaded silk fibroin (SFH) films. The physical properties including morphologies, contact angle, roughness, and Z range were characterized. MTS assay and fluorescence micrographs proved that SFH films inhibited the proliferation rate of HeLa cells due to induction of HFE into SF films. Furthermore, cell apoptosis assay and cell cycle analysis confirmed that the apoptosis of HeLa cells resulted from SFH films. Therefore, SFH films designed in our study might be a promising candidate material for cancer therapy. Silk fibroin (SF) films loaded with honeysuckle flowers extract (HFE) induced apoptosis of HeLa cells. Therefore, SFH films designed in our study might be a promising candidate material for cancer therapy.

Beschreibung: In this study, the change in the amounts the gunshot residues (GSR) collected by the “swab” method from shooter's right hand was analyzed using Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS) to test dependency on various conditions (such as skin color of the shooter, various physical properties, hair density of hands, size of the hands, sweaty or creamy (oiliness) hands). As a result of the analysis, there was no significant difference in the amount of GSR according to skin color, however, there was a change in the amount of GSR depending on the physical characteristics of the shooter. These results thought to have practical benefits for the experts performing GSR analysis using the swab technique to assess the incidence. In Microscopy Research and Technique sciences, chemical analysis has gained importance in the last decades. This result showed that the environmental conditions are very effective on the GSR. These analytic data will be beneficialabd useful in practice.

Beschreibung: In most mammalian species enamel prisms are regularly arranged in layers of alternating directions forming an angle of approximately 90°. These successive layers of prisms are known as Hunter–Schreger bands (HSBs). The analysis of HSBs may provide valuable information regarding the species life history, taxon and personal identification, with evident applicability in physical anthropology and forensics. Obtaining good quality digital images of HSBs in intact specimens is not always a feasible task. The major problems are the low contrast of images; the reflection of incident light, which may create areas of intense shine in digital images; and the abrupt decrease in the degree of illumination that occurs after light crosses the vertical cracks, frequently present in enamel. We show here that the area of intense shine can be minimized by a polarizing filter coupled to the camera objective, and the filling of enamel cracks with corn oil can reduce refraction of light in enamel cracks. These procedures can significantly increase the quality and the area of HSBs that can be recorded in intact teeth.

Beschreibung: To gain insight to underlying mechanism of the formation of calcium carbonate (CaCO 3 ) gallbladder stones, we did comparative study of stones with mud appearance and those with coralliform appearance. A total of 93 gallbladder stones with mud appearance and 50 stones with coralliform appearance were analyzed. The appearance, color, texture, and the detection of Clonorchis sinensis eggs by microscopic examination were compared between the two groups. Then, the material compositions of stones were analyzed using Fourier Transform Infrared spectroscopy and the spectrogram characteristics were compared. Moreover, microstructure characteristics of the two kinds of stones were observed and compared with Scanning Electron Microscopy. Mud-like gallbladder stones were mainly earthy yellow or brown with brittle or soft texture, while coralliform stones were mainly black with extremely hard texture, the differences between the two groups was significant ( p  〈 .05). The analytic results of FTIR spectroscopy showed that 95.7% (89/93) of the mud-like gallbladder stones were CaCO 3 stones, and mainly aragonite; while all of the coralliform stones were CaCO 3 stones, and mainly calcite ( p  〈 .05). Meanwhile, microscopic examination indicated that the detection rate of Clonorchis sinensis eggs in mud-like CaCO 3 stones was lower than that in coralliform CaCO 3 stones ( p  〈 .05), and that in aragonite CaCO 3 stones was lower than that in calcite CaCO 3 stones( p  〈 .05). Mud-like CaCO 3 stones mainly happened to patients with cystic duct obstruction. Clonorchis sinensis infection was mainly associated with coralliform (calcite) CaCO 3 stones. Cystic duct obstruction was mainly associated with mud-like (aragonite) CaCO 3 stones. Previous study showed that calcium carbonate stones were mainly mud-like or coralliform. The present study found that the appearance, color, texture, crystalline forms and the detection of C. sinensis eggs were different between the two kinds of stones. C. sinensis infection was mainly associated with coralliform (calcite) calcium carbonate stones. Cystic duct obstruction was mainly associated with mud-like (aragonite) calcium carbonate stones.

Beschreibung: Background Dentin hypersensitivity is an important clinical problem affecting a large percentage of the population, and various therapies have been suggested for its treatment—among them desensitizing agents and lasers. The aim of this study was to evaluate the in vitro effects of different in-office desensitizing agents and different type lasers, alone or in combination, on human dentinal tubules. Materials and Methods Eighty-four dentinal specimens obtained from freshly extracted impacted third molars were included and subsequently divided into seven groups: Group 1 (Control), Group 2 (Gluma desensitizer), Group 3 (Teethmate desensitizer), Group 4 (Nd:YAG laser), Group 5 (Er:YAG laser), Group 6 (Gluma desensitizer + Er:YAG laser), and Group 7 (Teethmate desensitizer + Er:YAG laser). The dentinal specimens of all groups were evaluated using SEM. A total of 12 images per sample were taken, and the numbers of open, partially occluded, and totally visible (open + partially occluded) dentinal tubules were counted. AFM was used to further substantiate the blocking mechanism. The data were analyzed by one-way ANOVA and Dunnett-C tests. Results Comparison of the number of occluded dentinal tubules per 100 µm 2 revealed that the laser and laser combined-treated groups showed the most occluded dentinal tubules. Gluma desensitizer + Er:YAG laser combination demonstrated significantly more tubule occlusion than all the other groups ( p  〈 .05). Conclusions The results of this study show that combined treatment methods were effective in the occlusion of dentinal tubules.

Beschreibung: Aged skin is reported to be associated with unattractive skin color changes and solar elastosis. However, comparative studies have not documented the possible correlation between the two factors. This study investigated the plausible relationship between the facial skin color of elderly Asians and solar elastosis. A total of 22 skin specimens were collected from 22 Korean patients who underwent cheek skin biopsies. Skin color was quantitatively measured using colorimetric photography techniques to produce CIE L * a * b * values; the degree of solar elastosis was quantifiably assessed using a histologic grading scale. These values were used to investigate a correlation between the CIE L * a * b * coordinates and solar elastosis grade. The solar elastosis grade increased according to patient age ( r  = 0.67, p  = .0006). However, the extent of solar elastosis was not statistically correlated with the CIE L * a * b * values, including L *, a *, and b * ( r  = 0.02, p = .95; r  = 0.15, p  = 0.50; r  = −0.07, p  = 0.76, respectively). The results showed that the solar elastosis grade increased, according to patient age, because of cumulative actinic damage. However, colorimetric skin color data did not correlate with the degree of solar elastosis. Therefore, cutaneous color changes and solar elastosis are separate, age-related phenomena. Physicians should be aware of the possible histologic changes in actinically damaged facial skin, regardless of the skin color. The solar elastosis grade increased, according to patient age; however, colorimetric skin color data did not correlate with the degree of solar elastosis. Elderly cutaneous color changes and solar elastosis are separate, age-related phenomena.

Beschreibung: Modern clinical studies have found that Pulsatilla radix contains a variety of active ingredients; however, its medicinal parts and microstructure have been controversial. Based on morphological observation and microscopic identification of different structures, we chose Anhui, Henan, Jilin, and Shanxi Province to study Pulsatilla chinensis (Bunge) Regel populations. We specifically addressed different diameters and anatomical structures of different parts of underground organs. We also found that P. chinensis (Bunge) Regel had a “joint point” structure in its underground organ. Above the “joint point” is the rhizome, below the “joint point” is the root. The main medicinal organ is the rhizome. The protective tissues of the endothelial layer of P. chinensis (Bunge) Regel changed in the process of its development. The protective tissues were replaced by epidermis, cortex, endodermis, and phloem. With the secondary growth, the endothelial cells have been radial division. This study showed that morphology and microscopic identification was an important mean of medicinal material identifications, and it had the characteristics of a convenient, fast, and intuitive method to identify the composition and structural characteristics of P. chinensis (Bunge) Regel medicinal parts. Joint points were found in the underground medicinal organs of P. chinensis for the first time. Through the observation of P. chinensis anatomical structure, we found that its medicinal part consisted of roots and rhizomes.

Beschreibung: For the microvision system, a new autofocus evaluation function based on the Robert function is proposed by increasing the threshold value. Compared with the traditional evaluation function, the new focus function reduces the local extreme value and increases the steepness of the focusing curve. According to the characteristics of the focusing evaluation function, the focus curve can be divided into two stages: the gentle area and the steep area. In the gentle area, there will be set a large step-length to realize the fast search. In the steep area, the data will be fitted by Gauss method, and on the basis of the fitting results, the motor of microvision system was directly driven to achieve the focal plane and this method has been improved in real-time and accuracy. A new autofocus method is proposed by increasing the threshold value. The focus curve will be divided into two stages. Set a large step-length in gentle area to realize the fast search. The data be fitted by Gauss method in steep area.

Beschreibung: Anisopterous odonate male's secondary genitalia is a complex of several structures, among them the vesica spermalis is the most informative with important specific characters. The observation of those characters, mostly of membranous nature, is difficult in the Scanning Electron Microscope due to dehydration and metallization processes. In this contribution, we discuss a new and low cost procedure for the observation of these characters in the SEM, compatible with the most common agents used for preserving specimens. A new and low cost alternative for the study of characters from arthropod mixed membranous and hard cuticle structures in SEM is presented and discussed.

Beschreibung: To clarify the toxicological effects of metal oxide nanoparticles (NPs) on microorganisms with environmental relevance, it is necessary to understand their interactions. In this work, they were studied the effects and the morphological interactions of two metal oxide NPs (ZnO and TiO 2 ) with microorganisms, during aerobic treatment of wastewater. The effects were evaluated according to nutrient removal from wastewater, while morphological interactions were determined by three different techniques such as TEM, HAADF-STEM, as well as an elemental mapping. According to results about effects of both NPs, they inhibited the removal of organic matter and ammonia nitrogen, and enhanced the orthophosphate removal. Related to morphological interactions, the electron-dense material of both NPs was mainly observed bounded to cell membrane. In tests with ZnO NPs, it was also observed electron-dense material internalized in microorganisms without physical damage in cell membrane. The elemental mapping was useful to determine that the electron-dense material corresponded to Zn and Ti. Both interactions, internalization and attachment of NPs on cell membrane of microorganisms may trigger the negative effect in the removal of organic matter and nitrogen. ZnO and TiO2 NPs inhibited the removal of organic matter and ammonia nitrogen from wastewater. TEM, HAADF-STEM and elemental mapping were useful to identify the attachment and internalization of NPs in microorganisms.

Beschreibung: The practical need for a simple and reliable tool for routine size analysis of nanoparticles with diameters down to a few nm embedded in a polymer matrix motivated the development of a new approach. The idea underlying the method proposed in this work is to combine intensity thresholding and contrast fitting procedures in the same software for particle recognition and measurements of sizes and size distributions of nanoparticles in transmission and scanning transmission electron microscopy images. Particle recognition in images is performed in an interactive process of manual setting the numerical threshold level after image preprocessing. We show that fitting the calculated gray level distribution to the real images is able to provide a maximum accuracy in measurements of the particle diameters in contrast to thresholding approaches. The fitting procedure is applied in the vicinity of nanoparticle images with the mass-thickness, diffraction, and chemical contrast. The grayscale function associated to the nanoparticle thickness is described using polynomial with degree ⩾ 2 and undetermined coefficients. The program for particle detection and size measurement— An alyzer of Na noparticles ( AnNa )—has been written and is described here. It was successfully tested on systems containing Ag nanoparticles grown and stabilized in aqueous solutions of different polymers for biomedical use and is available from the authors. A novel method for measuring nanoparticle size distributions on (S)TEM images was developed and implemented in An alyzer of Na noparticles ( AnNa ) program. It combines manual thresholding for particle recognition and original contrast fitting to derive sizes.

Beschreibung: SEM and light microscopy are of special interest for biologist to observe various features of the living bodies. In the current study we observed the foliar epidermal micro-morphological characters of 44 grass species using SEM and Light microscopy to assess their taxonomic utility for taxonomists in the identification process. The aim of this study is to use the foliar epidermal structural variations in both upper and lower surfaces for identification of grasses. Significant diversity was observed in both qualitative and quantitative characters using SEM and Light microscopy. Variations were observed in stomatal number, size, guard cells shape, silica bodies, macro-hairs, micro-hairs, epidermal cell number, subsidiary cells, prickles, hooks, papillae, and short and long cells. A taxonomic key is prepared using these variations for the identification of grass species. Based on these findings, SEM and Light microscopy of foliar epidermal features can be of special interest for taxonomists in the identification of complex grass taxa. SEM and LM foliar epidermal micro-morphological studies are important for plant taxonomists and a tool for taxonomic identification of grasses. The SEM and LM studies could be used to make taxonomic keys for fast and accurate identification of grass taxa.

Beschreibung: On the cover: The cover image, by Chantelle Venter et al., is based on the Research Article Effects of metals cadmium and chromium alone and in combination on the liver and kidney tissue of male Spraque-Dawley rats: An ultrastructural and electron-energy-loss spectroscopy investigation , DOI 10.1002/jemt.22877 .

Beschreibung: The focus of this double-blind randomized study was on evaluating the effect of an aqueous extract of Mastruz ( Chenopodium ambrosioides L.) on the bone repair process in vivo. In total, 36 male Wistar rats were randomly selected for this study, and divided into 3 groups ( n  = 12): Group HS (Hemostatic Sponge), Group SM (Hemostatic Sponge with Mastruz) and Group BC (Blood Clot). In each animal, bone defects measuring 2 mm in diameter were performed in both tibias for placement of the substances. After 3 and 10 days, the animals were sacrificed, and the tissues were analyzed under an optical microscope relative to the following events: inflammatory infiltrate; necrosis; young fibroblasts; osteoclastic and osteoblastic activity; endosteal and periosteal bone formation; and bone repair. The results were assessed by using Kruskal–Wallis and Mann–Whitney tests ( p  〈 .05). Inflammatory infiltrate demonstrated difference between Groups SM and BC in the time interval of 3 days ( p  = .004); an event related to the presence of the fibrin sponge and liquid of the extract, which induced a foreign body initial reaction. The presence of young fibroblasts ( p  = .003), osteoclastic ( p  = .003), and osteoblastic ( p  = .020) activity was statistically significant between Groups HS and BC in the time interval of 10 days; performance was related to the presence of the sponge within bone. As regards injured bone tissue repair, Group SM demonstrated a higher level of regenerative capacity ( p  = 0.004), due to a larger quantities of endosteal and periosteal bone formation, demonstrated in Group SM. The aqueous extract of mastruz stimulated bone neoformation, presenting wound closure with bone tissue at the end of 10 days. The aqueous extract of Mastruz stimulated bone neoformation, and presented wound closure with bone tissue at the end of 10 days. The use of Mastruz extract is a highly promising method for obtaining rapid bone repair for use in clinical practice.

Beschreibung: Oxidative stress increase induces cellular damage and apoptosis activation, a mechanism believed to represent a final common pathway correlated to sarcopenia and many skeletal muscle disorders. The goal of this study is to evaluate if melatonin, a ROS scavenger molecule, is able to counteract or modulate myotube death. Here, differentiated C2C12 skeletal muscle cells have been treated with melatonin before chemicals known to induce apoptotic death and oxidative stress, and its effect has been investigated by means of morpho-functional analyses. Ultrastructural observations show melatonin protection against triggers by the reducing of membrane blebbing, chromatin condensation, myonuclei loss and in situ DNA cleavage. Moreover, melatonin is able to prevent mitochondrial dysfunctions which occur in myotubes exposed to the trigger alone. These findings demonstrate melatonin ability in preventing apoptotic cell death in skeletal muscle fibers in vitro, suggesting for this molecule a potential therapeutic role in the treatment of various muscle disorders. Chemical triggers induced skeletal muscle death Melatonin, a ROS scavenger molecule, is able to counteract mitochondrial damage Melatonin prevents apoptosis in C2C12 myotubes

Beschreibung: Organic silicon (OS), glucosamine sulfate (GS), and methylsulfonylmethane (MSM) have been related to bone and connective tissue health and have been considered as basic therapy for osteoarthrosis disorders. Therefore, the aim was to analyze the effect of the association of these three components in mandibular bone defects in rats. Nine rats were used for histocompatibility test. In each animal was implanted the composition (70% OS, 15% GS, 15% MSM) and gutta percha (control) under the dorsal subcutaneous tissue. The samples were collected at 7, 14, and 21 days post-surgery and inflammatory events analyzed. In sequence, the composition was engrafted in mandibular bone defects of nine rats; bone defects without treatment were the control group. Analyses were performed at 7, 14, and 28 days post-surgery and samples were evaluated by scanning electron microscopy (SEM). For the histocompatibility test, both groups had a moderate inflammatory process at 7 days post-surgery and mild inflammatory process at 14 and 21 days. But in SEM analysis, the composition promotes an extensive reabsorption in cortical and crest alveolar bone, and great tooth root reabsorption. In conclusion, although the composition had positive result in the histocompatibility test, its direct application in mandibular bone defects caused intense resorption. Organic silicon, glucosamine sulfate, and methylsulfonylmethane had positive result in histocompatibility test. Organic silicon, glucosamine sulfate, and methylsulfonylmethane direct application in mandibular bone defects caused intense resorption.

Beschreibung: This in situ study evaluated the tubular occlusion caused by 4% TiF 4 gel on the surface of eroded/abraded dentin. Sixty human dentin samples were eroded in vitro and assigned into six groups ( n  = 10) according to the in situ surface treatment and number of cycling days: 4% TiF 4 gel applied once (TiF 4 1), twice (TiF 4 2), or three times (TiF 4 3) followed by 2, 4, and 6 days of erosive/abrasive in situ cycling, respectively. Control groups (no treatment) were subjected to 2 (C1), 4 (C2), and 6 (C3) days of erosive/abrasive in situ cycling only. A seventh group ( n  = 10) was comprised by in vitro uneroded samples (UN), subjected to 6 days of in situ erosive/abrasive cycling. Each cycling day consisted on six erosive (0.5% citric acid, pH 2.6) and one abrasive events. Environmental scanning electron microscopy micrographs were taken. For all groups, blinded examiners assessed dentin tubules occlusion using visual scores (0—unoccluded, 1—partially occluded by granular deposits, 2—partially occluded by reduction in tubular lumen into diamond shape, 3—completely occluded) on images captured prior and after the in situ phase. Scheirer-Ray-Hare test demonstrated that treatments significantly affected tubule occlusion ( p  〈 .001). Dunn's test showed that tubule occlusion in TiF 4 3 was significantly higher than in C1. Tubule occlusion in remaining groups did not differ from that observed in groups TiF 4 3 and C1. Tubule occlusion was significantly higher after in situ phase. It may be suggested that TiF 4 , when applied three times, was able to positively change tubule occlusion of dentin samples. Application of 4% TiF 4 gel renders the dentinal tubules flattened and diamond-shaped. Although treatment with TiF 4 was not able to generate a complete tubular occlusion, it decreased the lumens diameter, which could reduce dentinal hypersensitivity.

Beschreibung: On the cover: The cover image, by Alejandro del Palacio et al., is based on the Research Article A new method using Scanning Electron Microscopy (SEM) for preparation of anisopterous odonates , DOI: 10.1002/jemt.22903 .

Beschreibung: The aim of this study was to analyse the interfacial micromorphology of total-etch adhesives and dentin structures different locations by using SEM. Standardized cylindrical cavities (3mm in diameter, 2mm deep) with all margins in dentin were prepared on occlusal and buccal surfaces of twenty extracted human third molars. A total-etch dentin adhesive system and a light-cure flowable composite (Filtek Ultimate Flowable, 3M ESPE, St. Paul, MN, USA) were used in this study. Micro-morphological SEM analysis of the marginal seal of the original tooth specimens was performed using high magnification of up to 1000×. In this study, we found the difference in interfacial micromorphology in dentin different locations. Also, marginal gap was found in both observed dentin area. Better understanding of complexity and three- dimensional variations of the tooth structure is important for prevention of clinical challenges such as postoperative sensitivity, marginal discoloration and secondary caries, which could be prevented by achieving of predictable and long-lasting adhesive bond. In this article, we report on the influence of dentinal orientation on the quality of adhesive bond between composite restorations and dentin different locations. This is important for understanding the occurrence and prevention of microleakage.

Beschreibung: Fibronectin is an extracellular matrix protein that is involved in cell adhesion, growth, migration, differentiation, and wound healing. Fibronectin coatings are currently used in many laboratories for biomedical and biotechnology purposes. In this study we have investigated the adhesion and mechanical properties of fibronectin coatings. The coatings were also used to study the role of the residence time and the influence of the loading rate in nonspecific interactions. The results showed that the adhesion force between silica and fibronectin increased with loading rate delivering similar values for residence times of 1 and 2 s. Further analysis indicated that the distance to the transition state was about 0.5 nm. Moreover, the adhesion force did not vary with the loading rate for contact time of 0 s. The unfolding of fibronectin domains also depended of the Dwell time (no unfolding events were observed for zero residence time). Applied loads of 2 nN were able to stretch the fibronectin layer up to 200 nm and to unfold the three fibronectin domains, which were similar for a Dwell time of 1 and 2 s. However, the unfolding length increased with loading rate: below 2.5 µm s −1 the obtained lengths matched the value of FN I (13.5 nm), while for higher speeds the measured values corresponded to the lengths of FN II (18 nm) and FN III (27 nm). This investigation has answered and opened new questions about the mechanical stability and function of fibronectin coatings. The results have also raised theoretical questions about the difference between specific and nonspecific interactions to be addressed in future work. Residence time plays a crucial role in non-specific Si 3 N 4 - fibronectin interactions. Adhesion forces between Si 3 N 4 and fibronectin rise with increasing loading rate. The unfolding length of fibronectin domains depends on the loading rate.

Beschreibung: Renal fibrosis is characterized by glomerulosclerosis and tubulointerstitial fibrosis and its pathogenesis is associated with the activity of mesenchymal cells (fibroblasts), being essentially characterized by a process of excessive accumulation resulting from the deposition of extracellular matrix components. The aim of this study was to characterize the morphological presentation of chronic and fibrotic lesions in the glomerular, tubular, interstitial, and vascular compartments in feline CKD, as well as the possible participation of myofibroblasts in renal fibrotic processes in this species. Cat kidneys were collected and processed according to the conventional techniques for light microscopy, circular polarization, immunohistochemistry, and electron microscopy. Fibrotic alterations were present in all compartments analyzed. The main findings in the glomerular compartment were different degrees of glomerular sclerosis, synechia formation, Bowman's capsule calcification, in addition to glomerular basement membrane thickening and pericapsular fibrosis. The tubulointerstitial compartment had intense tubular degeneration and the immunostaining in tubular cells for mesenchymal cell markers demonstrated the possibility of mesenchymal epithelial transition and consequent involvement of myofibroblasts in the development of interstitial tubule damage. Infiltration of inflammatory cells, added to vessel thickening and fibrosis, demonstrated the severity and role of inflammation in the development and perpetuation of damage. Thus, we may conclude that fibrotic lesions play a relevant role in feline CKD and the mechanism of perpetuation of these lesions need further elucidation regarding the origin and participation of myofibroblasts and consequent mesenchymal epithelial transition in this species. Histological changes are important for the morphological description of kidney changes in cats with chronic kidney disease. Myofibroblats may play an important role in interstitial fibrosis in the chronic kidney disease in cats.

Beschreibung: Murine models of osteoarthritis (OA) are increasingly important for understating pathogenesis and for testing new therapeutic approaches. Their translational potential is, however, limited by the reduced size of mouse limbs which requires a much higher resolution to evaluate their articular cartilage compared to clinical imaging tools. In experimental models, this tissue has been predominantly assessed by time-consuming histopathology using standardized semi-quantitative scoring systems. This study aimed to develop a novel imaging method for 3-dimensional (3D) histology of mouse articular cartilage, using a robotic system—termed here “3D histocutter”—which automatically sections tissue samples and serially acquires fluorescence microscopy images of each section. Tibiae dissected from C57Bl/6 mice, either naïve or OA-induced by surgical destabilization of the medial meniscus (DMM), were imaged using the 3D histocutter by exploiting tissue autofluorescence. Accuracy of 3D imaging was validated by ex vivo contrast-enhanced micro-CT and sensitivity to lesion detection compared with conventional histology. Reconstructions of tibiae obtained from 3D histocutter serial sections showed an excellent agreement with contrast-enhanced micro-CT reconstructions. Furthermore, osteoarthritic features, including articular cartilage loss and osteophytes, were also visualized. An in-house developed software allowed to automatically evaluate articular cartilage morphology, eliminating the subjectivity associated to semi-quantitative scoring and considerably increasing analysis throughput. The novelty of this methodology is, not only the increased throughput in imaging and evaluating mouse articular cartilage morphology starting from conventionally embedded samples, but also the ability to add the third dimension to conventional histomorphometry which might be useful to improve disease assessment in the model. High-throughput serial sectioning and imaging of mouse articular cartilage Visualization of articular cartilage degenerative changes at high resolution by autofluorescence microscopy Automated quantitative analysis of articular cartilage

Beschreibung: This article studies the morphological and mechanical features of multinuclear and mononuclear SW480 colon cancer cells by atomic force microscopy to understand their drug-resistance. The SW480 cells were incubated with the fullerenol concentrations of 1 mg/ml and 2 mg/ml. Morphological and mechanical features including the height, length, width, roughness, adhesion force and Young's modulus of three multinuclear cell groups and three mononuclear cell groups were imaged and analyzed. It was observed that the features of multinuclear cancer cells and mononuclear cancer cells were significantly different after the treatment with fullerenol. The experiment results indicated that the mononuclear SW480 cells were more sensitive to fullerenol than the multinuclear SW480 cells, and the multinuclear SW480 cells exhibited a stronger drug-resistance than the mononuclear SW480 cells. This work provides a guideline for the treatments of multinuclear and mononuclear cancer cells with drugs. Atomic force microscopy was used to study the effects of fullerenol on SW480 cells. The morphology/mechanical features of multi/mononuclear SW480 cells were studied. Multinuclear cells exhibited stronger drug-resistance than mononuclear cells.

Beschreibung: Light microscopy is an essential tool in histological examination of tissue samples. However, the required equipment for a correct and rapid diagnosis is sometimes unavailable. Smartphones and mobile phone networks are widespread, and could be used for diagnostic imaging and telemedicine. Macrovesicular steatosis (MS) is a major risk factor for liver graft failure, and is only assessable by microscopic examination of a frozen tissue section. The aim of this study was to compare the microscopic assessment of MS in liver allograft biopsies by a smartphone with eyepiece adaptor (BLIPS device) to standard light microscopy. Forty liver graft biopsies were evaluated in transmitted light, using an Iphone 5s and 4 different mini-objective, add-on lenses. A significant correlation was reported between the two different approaches for graft MS assessment (Spearman's correlation coefficient: r s  = 0.946; p  〈 .001). Smartphone with eyepiece adaptor had similar discriminatory power to identify MS in liver grafts than standard light microscopy. Based on these findings, a smartphone integrated with a low-cost eyepiece adaptor can achieve adequate accuracy in the assessment of MS in liver graft, and could be used as an alternative to standard light microscope when unavailable. MS is only assessable by microscopic examination of a tissue section. We found, in a series of liver biopsies, similar performances for MS assessment by a smartphone with eyepiece adaptor (BLIPS device) and by standard light microscopy.

Beschreibung: Vulvovaginal candidiasis (VVC) is a common gynecologic infection and it occurs when there is overgrowth of the yeast called Candida. VVC diagnosis is usually done by observing a Pap smear sample under a microscope and searching for the conidium and mycelium components of Candida. This manual method is time consuming, subjective and tedious. Any diagnosis tools that detect VVC, semi- or full-automatically, can be very helpful to pathologists. This article presents a computer aided diagnosis (CAD) software to improve human diagnosis of VVC from Pap smear samples. The proposed software is designed based on phenotypic and morphology features of the Candida in Pap smear sample images. This software provide a user-friendly interface which consists of a set of image processing tools and analytical results that helps to detect Candida and determine severity of illness. The software was evaluated on 200 Pap smear sample images and obtained specificity of 91.04% and sensitivity of 92.48% to detect VVC. As a result, the use of the proposed software reduces diagnostic time and can be employed as a second objective opinion for pathologists. Vulvovaginal candidiasis (VVC) is a common gynecologic infection. This research proposes a Computer Aided Diagnosis (CAD) software for automatic detection of VVC. The Proposed CAD software can improve the performance of VVC detection.

Beschreibung: Samples of polyglycolic acid PGA/polycaprolactone PCL copolymer absorbable suture materials, which is called Poliglecaprone PGC copolymer suture, were thermally treated by two different processes. The first method is the direct heat exposure, from 20 to 38 °C, in the surrounding medium. Which is an environmental study. The other method is the annealing process at temperatures ranged from 65, 80, 100, 120, and 140 °C, for two different duration periods 60 and 120 min. This is an extensive technical study. The refractive indices of thermally treated PGA/PCL copolymer sutures were measured using multiple-beam Fizeau fringes. The birefringence profiles across the suture cross-sections were studied for different annealing conditions. The effect of heat on the polarizabilty per unit volume, dielectric properties, and suture diameter were investigated. The activation energy for the heating processes was calculated using Arrhenius equation. Microinterferograms are given for illustration. Two methods of heat treatment were used. The thermally treated PGA/PCL copolymer sutures were measured using multiple-beam interference microscopy. The results throw light on the structural variations across the suture cross section, which is important in medical applications.

Beschreibung: The present study is intended to assess gymnosperms pollen flora of Pakistan using Light Microscope (LM) and Scanning Electron Microscopy (SEM) for its taxonomic significance in identification of gymnosperms. Pollens of 35 gymnosperm species (12 genera and five families) were collected from its various distributional sites of gymnosperms in Pakistan. LM and SEM were used to investigate different palyno-morphological characteristics. Five pollen types (i.e., Inaperturate, Monolete, Monoporate, Vesiculate-bisaccate and Polyplicate) were observed. Six In equatorial view seven types of pollens were observed, in which ten species were sub-angular, nine species were Traingular, six species were Perprolate, three species were Rhomboidal, three species were semi-angular, two species were rectangular and two species were prolate. While five types of pollen were observed in polar view, in which ten species were Spheroidal, nine species were Angular, eight were Interlobate, six species were Circular, two species were Elliptic. Eighteen species has rugulate and 17 species has faveolate ornamentation. Eighteen species has verrucate and 17 have gemmate type sculpturing. The data was analysed through cluster analysis. The study showed that these palyno-morphological features have significance value in classification and identification of gymnosperms. Based on these different palyno-morphological features, a taxonomic key was proposed for the accurate and fast identifications of gymnosperms from Pakistan. For the first time SEM and LM palyno-morphological features was used to identify gymnosperm flora of Pakistan. Using these palyno-morphological features a taxonomic key was prepared for fast, accurate and as an aid identification of gymnosperms. SEM and LM palyno-morphological features were found significant for the identification of gymnosperm species.

Beschreibung: Plant anatomy was examined for two clarinet reeds made out of Arundo donax by different means of microscopy: light microscopy, low-energy secondary electron scanning electron microscopy (SEM), backscattered electron SEM, and helium ion microscopy (HiM). The local indentation hardness H IT and Young's modulus E IT of different tissues on their cross sections were measured. A vascular bundle (Vb) ( H IT  = 60–100 MPa, E IT  = 1,500–2,000 MPa) that includes soft tissues of phloem and xylem and a vascular bundle sheath (Bs) ( H IT  = 300–500 MPa, E IT  = ∼7,000 MPa) form a pipe of the strong string along the longitudinal direction of the cane. This Vb/Bs string is connected transversally with a net of thin cell-walls of parenchyma cells (Pa) ( H IT  = 70–200 MPa, E IT  = 2,000–3,000 MPa) that also range along the longitudinal direction of the cane. It was turned out that the acoustic quality of a reed is mainly ascribed to the shape and configuration of Vb and the size of Pa. A reed where Vb bundles with continuous Bs rings are homogeneously distributed with higher proportion among a softer network of small Pa cells enables musical performance. Plant anatomy was examined for two clarinet reeds made out of Arundo donax by different means of microscopy, and local indentation hardness of different tissues was measured to understand the acoustic quality relationship to the structure.

Beschreibung: Medicaments essential for alleviation of diseases may sometime adversely affect dental health by eroding the enamel, owing to their acidic nature. It is therefore highly desirable to be able to detect these effects quickly and reliably. In this study, we evaluated the erosive capacity of four most commonly prescribed respiratory disease syrup medicaments on enamel using micro-energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Fifty-five enamel fragments obtained from 30 bovine teeth were treated with artificial saliva (S), acebrofilin hydrochloride (AC), ambroxol hydrochloride (AM), bromhexine hydrochloride (BR), and salbutamol sulfate (SS); by immersing in 3 mL of respective solutions for 1 min, three times a day at intervals of 1 hr, for 5 days. µ-EDXRF analysis of enamel surface did not reveal significant erosion caused by the medications. However, ATR-FTIR showed a detectable shift in the phosphate (PO 4 ) antisymmetric stretching mode (ν 3 ) at ∼985 cm −1 for AM, BR, and SS, indicating erosion. Multivariate statistical analysis showed that AC, AM, SS, and BR could be classified with 70%, 80%, 100%, and 100% efficiency from S (control), further highlighting the ability of ATR-FTIR to identify degree of erosion. This suggests ATR-FTIR may be used to rapidly and nondestructively investigate erosive effects of medicaments. Molecular scale monitor of the in vitro enamel erosion was obtained. A detectible shift in the phosphate band after demineralization was found. Multivariate statistical analysis allows a prompt monitoring of demineralization.

Beschreibung: Abstract: The problem of a bistable magnetoelastic beam under base excitation was discussed in [1] under the aspect of chaotic behaviour in mechanical systems. Three decades later the system was used in [2] to design an energy harvesting system which performs well under harmonic excitation for a broad range of excitation frequencies due to its bistability. The initial modeling was tailored to obtain a model with one degree of freedom based on the assumption that the magnetic force acts on the beam tip only. A more appropriate model can be found when considering a distributed force along the beam. The authors present the force distribution on a ferromagnetic beam due to the magnetic field of two permanent magnets. A semi-analytic method is used to compute the magnetic field. The force distribution can in future be used to derive a refined nonlinear dynamical model for the ferromagnetic elastic beam. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: We investigate the dynamics after loss of stability of the downhanging configuration of a fluid conveying tube with a small end mass and an elastic support. By varying the fluid flow rate and the stiffness and location of the elastic support, different degenerate bifurcation scenarios can be observed. In this article we investigate the bifurcating solution branches of the codimension 3 interaction between a Hopf bifurcation and a Bogdanov-Takens bifurcation. A complete discussion of the primary and secondary solution branches was already given by W. F. Langford and K. Zhan. After reducing the system to the three-dimensional Normal Form equations we apply a numerical continuation procedure to locate the expected higher order bifurcation branches and detect more complicated dynamics, like Shilnikov orbits. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: An schlanken, geneigten Zylinderstrukturen wie Kabeln von Schrägkabelbrücken und Hängebrücken, Hängern von Bogenbrücken oder an Abspannseilen kann bei gleichzeitigem Auftreten von Regen und Wind das hydroaeroelastische Phänomen der Regen-Wind induzierten Schwingungen beobachtet werden. Die komplexen Strömungsvorgänge des Phänomens bedingen die Entwicklung eines hybriden Modells. Diese halbanalytischen/halbnumerischen und halbexperimentellen Verfahren zeigen Grenzbereiche zwischen Numerik und Experiment, deren physikalisch sinnvolle Beschreibung und Erklärung nur mit Hilfe der Kombination von numerischen und experimentellen Untersuchungen gelingt. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: Creep groan of brake systems is a low frequency vibration phenomenon occurring at low speeds which can make passengers feel uncomfortable. This phenomenon is caused by the stick-slip-effect resulting in limit cycle oscillations with frequencies lower than 200 Hz. For the experimental investigation of this problem, an idealized brake test rig is designed concentrating on the investigation of the frictional contact by realizing low damping and small disturbances in the system. Different sensors are utilized in the test rig. Limit cycles and bifurcation effects can be observed in the experimental results. With respect to modeling, a one degree-of-freedom (DOF) model using Coulomb's friction law and a two DOF model using the bristle friction law are considered. In a comparative study of experimental and simulation results, the parameters of both friction laws can be identified from the dynamic experimental results, such as the static and dynamic friction coefficients, contact stiffness and Stribeck velocity. Experimental and theoretical results show a very good concordance. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: A small-deformation strain gradient plasticity (GP) model for single-crystals has been proposed in [1], including a grain boundary (GB) yield condition without hardening. It has been extended by a hardening term for the GBs after a comparison to discrete dislocation dynamics (DDD) results in [2]. Differences between the strain gradients of the GP results and the DDD results motivate the consideration of a non-quadratic defect energy [3] in the GP model. It is shown that the gradients in the GP model can be improved using an exponent different from two. Remaining discrepancies in the strain profiles, compared to the DDD results, are attributed to the neglect of the individual gradients of plastic slip and due to the lack of a mechanism for the misorientation-dependent elastic interactions of dislocations across GBs [4] in the GP model. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: Pluta microscope used to throw light on the effect of heat treatment time on the different properties of poly (ethylene terephthalate) PET fibers. PET fibers were annealed at times ranged from 5 to 30 min at different temperatures (150, 170, 190, 210°C) using two different processes (fast cooling and slow cooling processes) in air. The refractive indices, the shrinkage, orientation factor and crystallinity of PET fibers were determined for different annealing temperature during the short time treatment. The shrinkage percentage and degree of crystallinity increased with increasing the temperature and time of annealing. Microinterferograms are given for illustration. Characterizing the effect of different cooling processes on the optical and structural properties. Discussing the effect of annealing process on the different properties of PET fibers.

Beschreibung: Early screening of skeptical masses or breast carcinomas in mammograms is supposed to decline the mortality rate among women. This amount can be decreased more on development of the computer-aided diagnosis with reduction of false suppositions in medical informatics. Our aim is to provide a robust tumor detection system for accurate classification of breast masses using normal, abnormal, benign, or malignant classes. The breast carcinomas are classified on the basis of observed classes. This is highly dependent on feature extraction process. In propose work, a novel algorithm for classification based on the combination of top Hat transformation and gray level co-occurrence matrix with back propagation neural network. The aim of this study is to present a robust classification model for automated diagnosis of the breast tumor with reduction of false assumptions in medical informatics. The proposed method is verified on two datasets MIAS and DDSM. It is observed that rate of false positives decreased by the proposed method to improve the performance of classification, efficiently. An automated system for the classification of breast abnormalities patterns. Segmentation free classification scheme by calculating a mesh of salient descriptors.

Beschreibung: On the cover: The cover image, by Hacer Aksel et al., is based on the Research Article Efficacy of different irrigant protocols and application systems on sealer penetration using a stepwise CLSM analysis , DOI: 10.1002/jemt.22944 .

Beschreibung: The pollen morphology of some species of glamorous family Convolvulaceae from arid zones of Pakistan has been evaluated. The pollen grains were acetolyzed, measured, described qualitatively, and illustrated using Light microscopy and scanning electron microscopy (SEM). The examined species have differences in shape, size, polarity and exine ornamentation, confirming the eurypalynous character. The pollen types varies from psilate to echinate and colpi to tricolporate, tetracolporate and pantocolporate. Exine ornamentation was exposed as perforate, reticulate and echinate. Spine morphology and exine sculpture are vital for the distinction of species. Pollen fertility shows that selected plants species are well-known in the Arid Zone. A taxonomic key is prepared to use these variations in the identification of species. Statistical analysis by using the Software XLSTAT exhibited that some morphological features is the main characters in identification of the taxa. These studied taxa were separated from each other using cluster Analysis. Our results constructed on MVSP ver. 3.22 software analyses that show morphological explanation; thus, the results highlight the importance of pollen morphology in the identification and characterization of species of the family convolvulacae in arid zone. The pollen morphology of family Convolvulaceae from arid regions of Pakistan has been evaluated. Light microscopy and scanning electron microscopy (SEM) has been attempted for the first time in arid zone. A taxonomic key is prepared which provides aid in the identification of species. Results constructed on MVSP ver. 3.22 and XLSTAT software analyses.

Beschreibung: The native shape and intracellular distribution of newly synthesized DNA was visualized by correlative (light and electron) microscopy in ice embedded whole cells of Escherichia coli . For that purpose, the commercially available modified nucleoside triphosphate named BODIPY® FL-14-dUTP was enzymatically incorporated in vivo into the genome of E. coli mutant K12 strain, which cannot synthesize thymine. The successful incorporation of this thymidine analogue was confirmed first by fluorescence microscope, where the cells were stained in the typical for bodipy green color. Later the preselected labeled E. coli were observed by Hilbert Differential Transmission Electron Microscope (HDC TEM) and the distribution of elemental boron (contained in bodipy) was visualized at high-resolution by an electron spectroscopic imaging (ESI) technique. The practical detection limit of boron was found to be around 5 ∼ 10 mmol/kg in area of 0.1 μm 2 , which demonstrated that ESI is a suitable approach to study the cytochemistry and location of labeled nucleic fragments within the cytoplasmic chromosomal area. In addition, the fine cellular fibrous and chromosomal ultrastructures were revealed in situ by combing of phase-plate HDC TEM and ESI. The obtained results conclude that the correlation between fluorescent microscopy with phase-plate HDC TEM and ESI is a powerful approach to explore the structural and conformation dynamics of DNA replication machinery in frozen cells close to the living state. BODIPY ® FL-14-dUTP was enzymatically incorporated into the genome of E. coli K-12 and the elemental boron distribution was mapped by ESI. The native shape of newly synthesized labeled DNA in E. coli was visualized by correlative microscopy.

Beschreibung: Mitochondrial morphology is regulated by continuous fusion-and-fission events that are essential for maintaining normal function. Despite the prominence of mitochondrial function in energy generation and cell signaling, understanding of processes of fusion and fission dynamics has been hampered by the lack of high-resolution optical systems that accommodate live-cell imaging. We have examined different confocal modalities in terms of resolution and signal-to-noise ratio (SNR) in a point scanning confocal microscope with Airyscan super-resolution (AS-SR). Results indicated that Airyscan (AS) provided speed, super-resolution, and high SNR. This modality was then used for monitoring mitochondrial dynamics in live tumor cells modified to harbor green-fluorescent protein localized to mitochondria. We then compared regular AS and fast-Airyscan modalities in terms of gentleness on the live-cell samples. The fast mode provided unprecedented imaging speed that permits monitoring dynamics both in 2D and also in three-dimensional dataset with time lapses (4D). Alterations to the mitochondrial network in U87 glioblastoma cells occurred within seconds and the cells were not affected by modest inhibition of fission. The super-resolution permitted quantitative measurements of mitochondrial diameter with a precision that enabled detection of significant differences in mitochondrial morphology between cell lines. We have observed swelling of mitochondrial tubules in A549 lung cancer cells after 2 hr treatment with deoxynyboquinone, an ROS-generating pharmacologic drug. We also tested different 3D analytical parameters and how they can affect morphometric quantitation. The AS-SR imaging enabled high-speed imaging of mitochondrial dynamics without the compromise to cell morphology or viability that is common with conventional fluorescence imaging due to photo-oxidation. Airyscan super-resolution microscopy provided higher speed, resolution, and SNR than confocal in imaging mitochondrial dynamics and physiological responses in living tumor cells.

Beschreibung: Depending on the process parameters, the directional solidification (DS) of eutectic alloys leads to a fibrous or lamellar microstructure. A physically motivated creep model which was evaluated for a DS-eutectic with a fibrous microstructure is applied to a DS-eutectic with a lamellar microstructure. Creep curves are simulated and compared to experimentally measured ones. It is shown, that the simulation is in good agreement with the experiment. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: The algorithmic implementation of a constitutive model for fibre-reinforced materials with fibre-bending stiffness, in addition to the classical directional contributions of the fibres, is presented. Based on theoretical investigations presented in [1], the numerical solution of the underlying non-linear system of coupled partial differential equations by means of a mixed-type multi-field finite element approach is discussed. To this end, additional field variables are introduced to approximate higher-order gradients which enter the proposed energy function of the extended continuum and, accordingly, introduce a size effect into the model. Concluding, representative boundary value problems are solved to analyse the basic properties of the model. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: Rotors in electrical machines are supported by various types of bearings. In general, the rotor bearings have nonlinear stiffness properties and they influence the rotor vibrations significantly. In this work, this influence of these nonlinearities is investigated. A simplified finite element model using Timoshenko beam elements is set up for the heterogeneous structure of the rotor. A transversally isotropic material model is adopted for the rotor core stack. Imposing the nonlinear bearing stiffnesses on the model, the Newton-Raphson procedure is used to carry out a run up simulation. The spectral content of these results shows nonlinear effects due to the bearings. The rotor vibrations are further investigated in detail for various constant speeds. These results show non-harmonic vibrations of the rotor in a section of the investigated speed range. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: Worldwide, breast cancer (BrCa) is currently the leading cause of deaths associated to malignant lesions in adult women. Given that some studies have mentioned that peritumoral adipocytes may contribute to breast carcinogenesis, present work sought to quantitative evaluate the morphometry of these cells in a group of adult women. Three thousand six hundred sixty four breast adipocytes, that came from biopsies of a group of adult females with different types of breast carcinomas (ductal, lobular, and mixed) and one with normal tissues, were evaluated through an image analysis (IA) process regarding six morphometric descriptors: area (A), perimeter (P), Feret diameter ( F D ), aspect ratio (AR), roundness factor (RF), and fractal dimension of cellular contour (FD C ). Data showed that the adipocytes of the normal tissues group were bigger (A: 3398 ± 2331 µm 2 , P: 239 ± 83 µm, and F D : 79.9 ± 24.5 µm) than those from BrCa samples (A: 2860 ± 1933 µm 2 , P: 214 ± 66 µm, and F D : 73.2 ± 22.5 µm), and presented a more irregular contour (FD C of 1.370 ± 0.037 for normal group and of 1.335 ± 0.049 for the oncologic one). Moreover, it could be accounted that adipocytes of mixed carcinomas were largest ( F D : 75.1 ± 22.4 µm) than those of lobular lesions ( F D : 61.6 ± 22.6 µm), while the adipocytes of ductal carcinomas were the most oval (AR: 1.421 ± 0.524) and roughest (FD C : 1.324 ± 0.050) cells. IA results suggest that BrCa lesions can be categorized through a quantitative morphometric evaluation of peritumoral adipocytes. These findings could let the development of an analytical tool to help the Pathologist to enhance the accuracy of the oncologic diagnose. Peritumoral adipocytes could be classified based on the morphometric analysis. Image analysis could be helpful in the Pathologic diagnostic of breast cancer. Principal component analysis was successful to classify breast cancer lesions.

Beschreibung: The purpose of this study was to compare the efficacy of different chelating solutions (17% EDTA and 10% citric acid) on the smear layer removal, and their effect on tubular dentin sealer penetration. Sixty root canals were prepared and distributed into four groups ( n  = 15) according to the final irrigation protocol: G1, final irrigation with 2.5 mL of distilled water; G2, final irrigation with 2.5 mL of 2.5% sodium hypochlorite solution; G3, final irrigation with 2.5 mL of 17% EDTA; and G4, final irrigation with 2.5 mL of 10% citric acid. Five specimens from each group were not filled to assess smear layer removal by scanning electron microscopy. Ten specimens from each group were filled for analysis of sealer penetration into dentinal tubules by confocal laser scanning microscopy. Smear layer removal (Kruskal–Wallis and Dunn's tests) and sealer penetration (F and Tukey's tests) were statistically analyzed with 95% of significance level. G3 and G4 had greater smear layer removal rates in the cervical and middle thirds, in comparison with G1 and G2 ( p  〈 .05). G3 and G4 had the highest percentages of sealer penetration in all thirds, in comparison with G1 and G2 ( p  〈 .05). Smear layer removal was effective only at the cervical and middle thirds when the chelating solutions were used. Sealer penetration into the dentinal tubules significantly increased in all root thirds when the specimens were treated with both chelating solutions. Smear layer removal was effective only at the cervical and middle thirds. Sealer penetration into the dentinal tubules may be correlated to the results observed in smear layer removal only for the cervical and middle thirds.

Beschreibung: This research aims to probe the porosity profile and polymerization shrinkage of two different dual cure resin cements with different dentin bonding systems. The self-adhesive resin cement RelyX U200 (named RU) and the conventional Allcem Core (named AC) were analyzed by x-ray microtomography (μCT) and Scanning Electron Microscopy (SEM). Each cement was divided into two groups ( n  = 5): dual-cured (RUD and ACD) and self-cured (RUC and ACC). μCT demonstrated that the method of polymerization does not influence the porosity profile but the polymerization shrinkage. Fewer concentration of pores was observed for the conventional resin cement (AC), independently the method used for curing the sample. In addition, SEM showed that AC has more uniform surface and smaller particle size. The method of polymerization influenced the polymerization shrinkage, since no contraction for both RUC and ACC was observed, in contrast with results from dual-cured samples. For RUD and ACD the polymerization shrinkage was greater in the lower third of the sample and minor in the upper third. This mechanical behavior is attributed to the polymerization toward the light. µCT showed to be a reliable technique to probe porosity and contraction due to polymerization of dental cements. The µCT showed to be a useful technique to study the polymerization contraction of dental materials. The not self-adhesive cement showed mechanical properties that might be desirable for the use in indirect restoration procedures.

Beschreibung: The aim of this study was to evaluate the effect of 24% ethylenediaminetetraacetic acid (EDTA) gel and 2% chlorhexidine gel (CHX) in dentin permeability and smear layer removal from root canals instrumented with NiTi rotary system using histochemical staining and scanning electron microscopy (SEM) analysis. Overall, 43 premolars were classified into two experimental groups, EDTA ( n  = 20) and CHX ( n  = 20), and a negative control (NC) ( n  = 3). All specimens were instrumented and the irrigant solutions were used after each file change. The EDTA group received a final rinse with 5-ml 1% NaOCl followed by a 5-ml 0.9% saline solution; the CHX group received a final rinse with 10-ml 0.9% saline solution; and the negative control group received a final rinse with only 0.9% saline solution. Fifteen teeth from each group were prepared for histochemical staining and evaluation of dentin permeability using the image-scanning software Axion Vision (v.4.8.2). Five remaining teeth were prepared for analysis using SEM for morphological analysis. The study found that 24% EDTA gel increased the permeability of dentin in all thirds evaluated and also demonstrated an increased cleaning ability, with dentinal walls free of smear layer and open dentinal tubules, as compared to 2% CHX gel. It was concluded that EDTA was efficient in cleaning the dentinal tubules and increased dentin permeability. Best results occurred with 24% EDTA gel which increased dentin permeability in all thirds evaluated, and presented greater cleaning ability, with dentinal walls free of smear layer and open dentinal tubules, which did not occur with 2% CHX gel.

Beschreibung: This study aimed to test the hypotheses that (i) a parameter related to permeability, α d (ratio of squared water volume by the nonmineral volume) is, among all major component volumes (mineral, water, and organic volumes) the best predictor of quinoline infiltration in natural enamel caries (NEC), and (ii) the pore volume fraction infiltrated by quinoline ( V qui ) in NEC is much lower than previous estimates that neglected water and organic enamel volumes. Mineral and nonmineral volumes and α d were measured at 341 histological points (from 20 approximal NEC lesions), and transport of quinoline was tracked by orientation-independent polarizing microscopy. R 2 values of V qui were 0.596 (α d ), 0.033 (mineral volume), 0.474 (water volume), and 0.011 (organic volume). V qui values were 23% (body of the lesion), 7% (dark zone), and 9% (translucent zone), lower than previous estimates (with high effect size). Transport of quinoline occurred both parallelly and perpendicularly to prism paths, and dark zones were seen where only transport parallel to prisms occurred. In conclusion, α d was the main predictor of quinoline infiltration, but it differed from the water volume with a small effect size, and the pore volume fraction with quinoline was much lower than previous estimates. Volume of oil infiltrated in enamel caries is presented for histological zones relevant for progress and reversal of caries. Oil infiltrated 〈 50% of the enamel pore volume and it is well correlated with permeability but not with mineral content.

Beschreibung: On the cover: The cover image, by Maria Sumarokova et al., is based on the Research Article Adhesion, unfolding forces, and molecular elasticity of fibronectin coatings: An atomic force microscopy study , DOI: 10.1002/jemt.22954 .

Beschreibung: Three-dimensional (3D) printing technology has the advantage of enabling specific visualization of creative ideas. Since synchrotron based images can provide high sensitivity and high resolution, they are a very useful technology to identify the 3D anatomy of microscale samples. X-ray images using such synchrotron radiation are grafted to 3D printing technology. We can be obtained 3D images and modeling data of an ant using synchrotron radiation, and then, it were outputted with the 3D printer. A new way to identify the usefulness of the structure analysis is then found by visualizing the micro-internal structure of diverse biomedical samples and creating an enlarged model. This study suggests methods of utilizing a 3D printed model produced through synchrotron radiation imaging in various fields such as bioengineering, medical, and education. We suggest a new way to identify the usefulness of the structure analysis by using 3D printing technology and synchrotron radiation beam. This study shows methods of utilizing by making a 3D model that shows the micro-internal structure of insects.

Beschreibung: Seed micromorphology of 12 species of tribe Vicieae (Papilionoidae) representing five genera were examined using Scanning Electron Microscope (SEM). The different seed types were described, illustrated, compared, and their taxonomic importance is discussed. Seeds exhibit great diversity in ultrastructure and a variety of novel morphological features have been determined among and within genera of the tribe. Seeds were characterized by oval to round shape, ovate to oblong outline and striate to papillate ornamentation. Radiate and wavy cell pattern has been observed as a characteristic exomorphological feature. In Vicia sativa , testa cells superficially seem to form peltate hairs. As a result, seed micromorphology with few exceptions showed variation and its taxonomic value was significant in distinguishing taxa at species level. The disparity in shape and density of papillae or protuberances and alignment of testa cells, may possibly give further insight at intraspecific level within tribe. The findings also illustrated that the use of SEM in seed morphology can reveal hidden morphological affinities among species and showed potential in delimitation of Vicieae members generally at tribe, genus, and particularly at species level. This study is focused on seed morphological and ultrastructural aspects in five genera and 12 species of tribe Vicieae . The seeds were characterized by oval to round shape, ovate or oblong outline, striate to papillate ornamentation, and wavy cell margin. Two distinctive cell patterns were observed; radiate and wavy, the testa cells in Vicia sativa superficially seem to form peltate hairs.

Beschreibung: The purpose of this study was to evaluate age- and sex-related changes in the anatomical relationships between the roots of the molars and premolars and the mandibular canal using cone-beam computed tomography (CBCT) images. A total of 243 patients (116 males, 127 females) aged 16–83 years for whom previous CBCT scans were available were enrolled in this study. The patients were subcategorized by sex and age (Group I, 〈21 years; Group II, 21–40 years; and Group III, 〉40 years). The distances between the mandibular canal and the molars and premolars were measured. The mandibular canal was significantly closer to the root apices of the second and third molars than to the apices of other evaluated teeth ( p  〈 0.05). All measurements were significantly higher in male than in female subjects ( p  〈 0.05). Group I subjects exhibited significantly shorter distances between the mandibular canal and root apices of the first and second premolars, and the molars, than those of Group II and III subjects ( p  〈 0.05). Direct contact relationships were found in 1.6%, 3.3%, 3.3%, 16%, and 32.2% of teeth (running from the first premolar to the third molar, respectively). Age and sex influenced the anatomical relationships between mandibular teeth and the mandibular canal, and these should be considered when planning endodontic and surgical procedures to avoid potential nerve injury. CBCT was used to evaluate the proximity of the apices of teeth to the inferior alveolar nerve. Age and sex influence the anatomical relationships between mandibular teeth and the mandibular canal. Our current data provides useful anatomical information to clinicians.

Beschreibung: This contribution is concerned with the computational analysis of a rigid rotor supported by means of two self-acting foil air journal bearings. Even though the overall equation system is thereby typically written in a nondimensional form, prior knowledge about realistic value ranges of occurring dimensionless numbers is required in order to parameterize and interpret such simulations correctly. Unlike all other quantities, the nominal lubrication gap clearance between the rotating journal and the undeformed foil structure is reported to be only poorly known. Thus, even in the light of an advanced understanding of the bearing rotor system's fundamental behavior, the quantitative reproduction and prediction of experimental results by means of computational analysis need to be viewed critically. In this study, the sensitivity of numerical results towards the assumed nominal lubrication gap clearance will be investigated. To this end, the stability of the system is considered and the characteristics of occasionally observed equilibrium points and limit cycles are addressed. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)

Beschreibung: Mosquito-borne diseases, such as malaria, dengue fever, and Zika virus, are serious global health issues. Vector control may be an important strategy in reducing the mortality caused by these diseases. The respiratory system of mosquito larvae in the water has to inhale atmospheric oxygen as aquatic organisms. In this study, the three-dimensional (3D) structures of the dorsal longitudinal trunks (DLTs) of the tracheal systems of Anopheles sinensis and Aedes togoi were compared using synchrotron X-ray microscopic computed tomography. DLT respiratory frequencies were also investigated. Interestingly, the larvae of the two mosquito species exhibit tracheal systems that are both morphologically and functionally distinct. A. sinensis hangs horizontally under the water surface, and has a smaller DLT volume than A. togoi . In contrast, A. togoi hangs upside down using a siphon by fixing its tip to the water surface. The frequency of peristaltic movement in A. togoi is higher than that of A. sinensis . These differences in the structures and breathing behaviors of the respiratory systems of mosquito larvae provide new insights into the tracheal systems of mosquito larvae, which should help develop novel effective control strategies targeting mosquito larvae. The dorsal longitudinal trunks (DLT) of the tracheal systems of A. sinensis and A. togoi larvae have distinct morphological features. Aedes togoi larvae may have a much longer underwater stay with a larger tracheal system volume and a lower frequency of peristaltic movement than A. sinensis larvae.

Beschreibung: It was known that Spinal cord injury (SCI) leads to neuronal cell death, axonal damage and demyelination, and often results in severe functional loss. Noninvasive imaging of axonal integrity may hold great importance in clinic. This study evaluated the role of phase imaging in detection of axonal integrity of cranial corticospinal tract after spinal cord injury. Quantitative MR phase imaging and diffusion tensor imaging were performed on a group of rats prior to and after spinal cord injury at 9.4 T in this study. The phase contrast decreased mainly in contralateral white matter containing the corticospinal tract (CST), i.e., pyramid by 46%, two weeks postinjury compared to preinjury levels. Diffusion measurements further showed fractional anisotropy (FA) increased and radial diffusivity (λ⊥) decreased significantly, and axial diffusivity (λ∥) remained unchanged in these regions confirmed intact fiber tracts two weeks after injury, precluding the possibility of axonal damage and potential contribution to the shifted susceptibility. It was concluded that phase imaging is a potential endogenous biomarker for brain axonal integrity after spinal cord injury. This novel tool could potentially be applied to diagnose brain pathologies in patients who have spinal cord injury. Phase imaging is a potential endogenous biomarker for brain axonal integrity after spinal cord injury. This novel tool could potentially be applied to diagnose brain pathologies in patients who have spinal cord injury.

Beschreibung: Aim : Evaluate the composite-to-enamel bond after passive or active application of self-etching primer systems on polished or pre-etched enamel with phosphoric acid. Materials and Methods : Two self-etch adhesives systems (SEAS) were used: Clearfil SE Bond and Easy Bond. Third human molars were divided into 8 groups ( N  = 10). The crown of each tooth was sectioned into halves and the mesial/distal surfaces were used. The adhesives were actively or passively applied on enamel with or without prior phosphoric-acid etching. Resin composite cylinders were built after adhesive application. After stored in relative humidity for 24 hr/37°C the specimens were subjected to microshear test in universal testing a machine at a crosshead speed of 0.5 mm/minute. The results were analyzed with three-way ANOVA and the Tukey test. The enamel-etching pattern was evaluated under SEM. Results : The 2-step SEAS system presented significantly higher adhesive bond strength means (47.37 MPa) than the 1-step (36.87 MPa). A poor enamel- etching pattern was observed in active mode showing irregular and short resin tags, however there was not compromised the bond strength. Conclusion : Active or passive application produced similar values of bond strength to enamel regardless of enamel pretreatment and type of SEAS. It is still no consensus on the techniques that improve adhesion of self-etch adhesives to enamel. The images produced by SEM showed that the etching pattern of enamel obtained after active or passive application did not influence the bond strength.

Beschreibung: The modified acid fast staining technique is a commonly used procedure for the detection of coccidian parasites in developing countries. The morphological variations observed in these parasites play a significant role to some extent in both identification and diagnosis of these parasitic infections. A prospective cross sectional study was performed over three years. The fecal smears were stained by modified Kinyoun acid-fast staining technique and were extensively studied for morphological variations in the coccidian parasites. Out of a total of two thousand one hundred fifty one ( n  = 2,151) fecal samples received during the study period, 259 samples (12%) were positive for any one of the coccidian parasites. Morphological variations, especially in the staining character was noted in all the three coccidian parasites. This study was an attempt to characterize different variations in size, shape and staining characteristics of the three coccidian parasites. The most commonly observed forms in Cryptosporidium spp., Cyclospora cayetanensis and Cystoisospora belli were the erythrocytic forms, soap bubble forms and the completely stained immature unsporulated forms respectively.

Beschreibung: This study evaluated four root repair materials for cytotoxicity and cell attachment in vitro. Cell viability was determined at 24 hr, 3 days and 7 days by using a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay with material extracts. Cell adhesion was examined with a scanning electron microscope on the surface of materials at 24 and 48 hr. Angelus MTA displayed the lowest cell viabilities at all-day incubations. Endocem had high biocompatibility on the first day. After culturing for 3 days and 7 days, the cell viabilities of Biodentine, Endocem and MM-MTA had similar results, and their cell viability was significantly higher than that of Angelus. No definite relation was found between the incubation time and the relative cell viability in any group. In scanning electron micrographs, the cells were attached to the material surface for all materials, although the cells in the Biodentine group were attached better than the other groups on the second day. Cell viability and cell attachment was lower in the Angelus group. Endocem, Biodentine and MM-MTA were similar in biocompatibility and cytotoxicity. Root repair materials have been closely related in vital tissues and biocompatibility and cell attachment is necessary for them. Results of the present study show the direction to clinicians for chosing the right one.

Beschreibung: Serotonin [5-hydroxytryptamine (5-HT)] is an important neuromodulator involved in a wide range of physiological functions. The effects of serotonin are mediated by an extended family of receptors coupled to multiple heterotrimeric G-proteins, associated with cellular membrane. G proteins connect receptors to effectors and thus trigger intracellular signaling pathways. These cellular processes several regulate systemic functions such as embryonic development, gonadal development, learning and memory, and organismal homeostasis. Generally, elasmobranch fish dwell a hypersaline environment and utilize a specialized extrarenal salt secreting organ, the rectal gland, to face ionic homeostasis. In this study in addition to the morphological, histochemical and immunohistochemical description of the Scyliorhinus canicula rectal gland, for the first time, the presence of serotonin (5-HT), and distribution of different types of G protein alpha subunits (Gα o, Gα q/11, and Gα s/olf) has been investigated in the rectal gland epithelium by confocal immunofluorescence techniques. Colocalization G proteins and 5-HT in the secretory epithelium of the gland suggests serotonin acts as a hormone and involves G proteins in an autocrine-paracrine control of rectal gland homeostasis. G proteins are involved in autocrine-paracrine control of rectal gland homeostasis For the first time the presence of different types of G protein alpha subunits in the rectal gland epithelium has been investigated. Serotonin positivity in the secretory cells increases activation of the cyclic AMP (cAMP) system and the inositol (1,4,5)-trisphosphate/Ca 2+ system.

Beschreibung: The aim of this study was to investigate the effect of cyclosporine A (CsA) therapy on bone healing around osseointegrated implants in the rabbit by means of descriptive histologic and histomorphometric analyses. Eighteen rabbits received one implant each in the right tibia proximal metaphysis. After the bone-healing period of 3 months, 6 randomly selected animals were sacrificed (Group CTL-12) before the immunosuppression therapy initiation to obtain a standard of implant integration. Another six randomly selected animals were submitted to a daily subcutaneous ( sc ) injection of 10 mg/kg CsA (Group CsA) whereas the six remaining animals received daily sc injections of saline solution (Group CTL-24). Groups CTL-24 and CsA were sacrificed after another 12 weeks. Bone-to-implant contact (BIC), and bone area fraction occupancy (BAFO) within the implant threads were measured. Bone density (BD) and descriptive histological analyses were also accomplished. Rabbits under CsA therapy presented statistically lower percentage of BIC (33.28 ± 6.19) compared to the animals of CLT-24 (55.6 ± 17.73). No differences were found between CsA and CTL-12 (38.7 ± 7.87). Significant decreased percentage of BAFO between the implant threads in the CsA (48.43 ± 9.48) group compared to the CTL-24 (72.26 ± 11.72) was observed. No differences were found between CsA and CTL-12 (61.99 ± 13.94) groups. BD analysis showed significant lower BD in the CsA (48.56 ± 9.32) group compared to the CTL-12 (68.74 ± 10.89) and CTL-24 (77.96 ± 4.96) groups. Collectively, our findings demonstrated that CsA therapy negatively affect the bone healing around osseointegrated implants due to the significant lower values for BIC, BAFO, and BD. CsA administration negatively affect the bone healing around osseointegrated dental implants due to the decreased bone-to-implant contact, the lower percentage of bone area fraction occupancy, and reduced bone density around the implant threads.

Beschreibung: Background and Objectives The regeneration of periodontal support is the main concern in periodontal therapy. The aim of this in vitro study was to investigate the fibroblasts attachment on root surfaces after scaling with Er, Cr:YSGG laser and ultrasonic instruments using scanning electron microscopy (SEM). Methods 72 root plates of ∼6 × 4 × 1 mm 3 in dimension were prepared from 27 single-rooted human mature teeth and were then divided into four groups. One group irradiated with a G6 tip of Er, Cr:YSGG laser (2.78 µm, 0.75 W, pulse duration of 140 µs, repetition rate of 20 Hz) for 5 to 7 s, and the other groups were scaled with ultrasonic alone or laser-ultrasonic. The control group was subjected to neither laser nor ultrasonic scaling. Subsequently, Viability and proliferation rates were done using MTT assay on days 3 and 5. Finally the cell attachment was observed using SEM. Results The data derived from MTT and cell-attachment analysis indicated that laser-ultrasonic scaling tended to increase cell-viability by the lapse of time (within 3–5 days), with significantly better cell-attachment compared with other groups on days 3 and 5 ( p  〈 .05). The comparison of the difference in fibroblast cell attachment rate on both the third and the fifth day with independent T-Test indicated a significant rise on the fifth day compared to the third day of study ( p  〈 .05). Conclusion Indeed, both Er, Cr:YSGG laser and ultrasonic scaling may promote fibroblast attachment on dentinal root surfaces more than laser or ultrasonic scaling alone. Cell viability assay in laser-ultrasonic group was significant compared with Laser scaling alone on the fifth day. Scaling with laser-ultrasonic is more efficient than laser or ultrasonic scaling alone in enhancing the attachment of fibroblast.

Beschreibung: Objective This study aims at reducing frictional resistance of the ceramic brackets by using CO 2 laser irradiation. Methods Forty-two polycrystalline Al 2 O 3 ceramic brackets were randomly divided into six groups of seven samples: a control group B0 (not subjected to laser irradiation) and five groups subjected to irradiation with intensities of 10 (B10), 30 (B30), 70 (B70), 90 (B90), and 110 (B110) J/cm 2 . After irradiation, two samples from each group were evaluated by scanning electron microscopy (SEM) and atomic force microscopy (AFM), while the remaining five samples were tested for frictional resistance. The sliding friction of stainless steel wires (SS-wires) in the brackets was measured using a universal testing machine. Results Samples from the first set (groups B0, B10, and B30) were significantly more resistant to wire sliding than the samples from the second set (groups B70, B90, and B110). The SEM analysis shows different degrees of blister formation on the bracket surfaces subjected to laser irradiation and no changes in their grain sizes. The AFM results indicate more consistent blister formation for groups B70, B90, and B110 than for other groups. Conclusion Different CO 2 laser power intensities significantly affect frictional resistances of SS-wires in Al 2 O 3 ceramic brackets. This is the first study using a CO 2 laser to reduce frictional resistance between ceramic brackets and SS-wires. CO 2 laser irradiation of 70, 90, and 110 j/cm 2 reduced the sliding friction. SEM and AFM showed change in surface morphology of brackets.

Beschreibung: We investigated the association between human polymorphonuclear leukocytes (PMNs) and non-opsonized Tannerella forsythia ATCC 43037 displaying a serum-resistant surface layer (S-layer). When PMNs were mixed with T. forsythia in suspension, the cells phagocytosed T. forsythia cells. Nitro blue tetrazolium (NBT) reduction, indicative of production, was observed by light microscopy; cerium (Ce) perhydroxide deposition, indicative of H 2 O 2 production, was observed by electron microscopy. We examined the relationship between high-molecular-weight proteins of the S-layer and Ce reaction (for T. forsythia phagocytosis) using electron microscopic immunolabeling. Immunogold particles were localized within the PMNs and on cell surfaces, labelling at the same Ce-reacted sites where the S-layer was present. We then used energy dispersive spectroscopy (EDS)-scanning transmission electron microscope (STEM) to perform Ce and nitrogen (N) (for S-layer immunocytochemistry) elemental analysis on the phagocytosed cells. That is, the elemental mapping and analysis of N by EDS appeared to reflect the presence of the same moieties detected by the 3,3′-diaminobenzidine-tetrahydrochloride (DAB) reaction with horseradish peroxidase (HRP)-conjugated secondary antibodies, instead of immunogold labeling. We focused on the use of EDS-STEM to visualize the presence of N resulting from the DAB reaction. In a parallel set of experiments, we used EDS-STEM to perform Ce and gold (Au; from immunogold labeling of the S-layer) elemental analysis on the same phagocytosing cells. We investigated the association between leukocytes and T. forsythia ( T.f .) by EDS-STEM. We detected Ce, H 2 O 2 production, and nitrogen (S-layer from T. f , DAB with HRP instead of immunogold labeling) elements on the same sites of leukocyte cytoplasm.

Beschreibung: Collagen often acts as an extracellular and intracellular marker for in vitro experiments, and its quality defines tissue constructs. To validate collagen detection techniques, cardiac valve interstitial cells were isolated from pigs and cultured under two different conditions; with and without ascorbic acid. The culture with ascorbic acid reached higher cell growth and collagen deposition, although the expression levels of collagen gene stayed similar to the culture without ascorbic acid. The fluorescent microscopy was positive for collagen fibers in both the cultures. Visualization of only extracellular collagen returned a higher correlation coefficient when comparing the immunolabeling and second harmonic generation microscopy images in the culture with ascorbic acid. Lastly, it was proved that the hydroxyproline strongly contributes to the second-order susceptibility tensor of collagen molecules, and therefore the second harmonic generation signal is impaired in the culture without ascorbic acid. The VICs markers were identified together with the impact of ascorbic acid deficiency on the quality of collagen fibers. The conventional techniques were positive about deposited collagen; however, no SHG signal was detected.

Beschreibung: The aim of this study was to evaluate the antibacterial activity of a composite resin modified by 3D zinc oxide (ZnO) microstructures and to verify possible alterations on its mechanical properties. ZnO was synthesized by hydrothermal approach and characterized by X-ray diffraction (XRD), surface area by Brunauer, Emmett and Teller (BET), Fourier transform infrared spectroscopy (FTIR) and Field emission scanning electron microscopy (FESEM). The minimum inhibitory concentrations of ZnO against Streptococcus mutans, Escherichia coli, Staphylococcus aureus , and Candida albicans were determinated. The composite resin Filtek TM Z350XT (3M of Brazil) was blended with 0.2%, 0.5%, and 1% in weight of ZnO and submitted to antibacterial assay by direct contact test against S. mutans , the leading cause of dental caries and the most cariogenic oral streptococci. Additionally, it was performed compressive and diametral tensile strength tests of the modified composite resin. Microrods and hollow microrods of ZnO were obtained and its MIC values were found to be 125 μg/mL for S. mutans , 500 μg/mL for C. albicans and 62.5 μg/mL for S. aureus . For the tested concentrations, it was not found MIC against E. coli . The direct contact test showed a significant antibacterial capacity of modified composite resin ( p  〉 0.05 for all concentrations). The compressive and diametral tensile strength remains no changed after inclusion of microparticles ( p  〉 0.05 for all concentrations). The modification of the composite resin with small amounts of ZnO microparticles significantly inhibited the S. mutans growth on resin surface without significant alterations of its mechanical strength. ZnO hollow microtubes with hexagonal structures was synthesized. The ZnO microparticles were efficient against S. mutans , C. albicans and S. aureus . The composite resin modified by ZnO inhibited S. mutans growth on its surface.

Beschreibung: The aim of this study was to evaluate the sealing ability and marginal adaptation of calcium silicate-based cements (CSCs) in root-end cavities prepared by ultrasonic and laser tips. A total of 72 extracted human maxillary incisor teeth were randomly divided as 60 teeth in experimental groups and 6 teeth each for positive and negative control groups. Specimens in experimental groups were obturated, their root-end resections were performed and randomly divided into six groups ( n  = 10) as follows: G1: Ultrasonic retrotip + MTA, G2: Ultrasonic retrotip + Calcium Enriched Mixture (CEM), G3: Ultrasonic retrotip + Biodentine, G4: Er:YAG laser tip + MTA, G5: Er:YAG laser tip + CEM, G6: Er:YAG laser tip + Biodentine. The sealing ability was measured by fluid transport method. Six specimens from each experimental group were randomly selected to analyze marginal adaptation and prepared for scanning electron microscopy (SEM) analysis. Micrographs were scored and also analyzed using Image J software. Data were analyzed with; two-way ANOVA, Bonferroni, Kruskall–Wallis, Mann–Whitney-U, Siegel & Castellan, and Spearman correlation coefficient tests. No significant difference was found between materials regarding the sealing ability and marginal adaptation ( p  〉 0.05). Significantly greater fluid movement and poor marginal adaptation were seen for materials placed in cavities prepared by laser tips ( p  〈 0.05). Positive correlation was found between the results of scoring and Image J analysis of SEM images ( r  = 0.596, p  〈 0.001). Fluid transport method and SEM analysis gave similar results suggesting the use of ultrasonic-retrotips for preparing root-end cavities which are going to be filled with one of these CSCs. All materials presented similar sealing ability and adaptation. The use of ultrasonics was associated with better results than the use of Er:YAG laser. A correlation was found between the qualitative and quantitative analysis of SEM images.

Beschreibung: Quantification of intestinal mucosal growth typically relies on morphometric parameters, commonly villus height, as a surrogate for presumed changes in mucosal surface area (MSA). We hypothesized that using mathematical modeling based on multiple unique measurements would improve discrimination of the effects of interventions on MSA compared to standard measures. To determine the ability of mathematical modeling to resolve differences in MSA, a mouse model with enhanced serotonin (5HT) signaling known to stimulate mucosal growth was used. 5-HT signaling is potentiated by targeting the serotonin reuptake transporter (SERT) molecule. Selective serotonin reuptake inhibitor-treated wild-type (WT-SSRI), SERT-knockout (SERTKO), and wild-type C57Bl/6 (WT) mice were used. Distal ileal sections were H&E-stained. Villus height (VH), width (VW), crypt width (CW), and bowel diameter were used to calculate surface area enlargement factor (SEF) and MSA. VH alone for SERTKO and SSRI was significantly increased compared to WT, without a difference between SERTKO and WT-SSRI. VW and CW were significantly decreased for both SERTKO and WT-SSRI compared to WT, and VW for WT-SSRI was also decreased compared to SERTKO. These changes increased SEF and MSA for SERTKO and WT-SSRI compared to WT. Additionally, SEF and MSA were significantly increased for WT-SSRI compared to SERTKO. Mathematical modeling provides a valuable tool for differentiating changes in intestinal MSA. This more comprehensive assessment of surface area does not appear to correlate linearly with standard morphometric measures and represents a more comprehensive method for discriminating between therapies aimed at increasing functional intestinal mucosa. Research Highlights Evaluation of changes to the intestinal mucosa commonly relies on histologic measures. Using a novel and accurate method for calculating mucosal surface area, we found significant differences between experimental groups which were missed by standard measures alone.

Beschreibung: To evaluate the performance degradation of fabric drying in domestic air-vented dryer, the appearance, mechanical, and microscopic properties of the wool fabric after different drying cycles were studied. Pilling and dimensional stability tests were performed according to established international standards. Microscopic studies were carried out by Scanning Electron Microscopy (SEM) and X-ray (XRD). The results show that pilling is the dominant damage mechanism and gradually increased during lower number of drying cycles (0–10 cycles). The results of dimensional stability showed that the highest dimensional shrinkage of knit wool fabric was first five drying cycles, and then increased at a relatively slow rate with the increase in drying cycles. And fabric shrinkage takes place mainly in the warp direction. The SEM images of wool fiber showed that the scales on the fiber surface were intact in the first 15 drying cycles but start to flake after 20 drying cycles. In the drying process, fabrics can suffer several fractures such as cut fracture, split fracture, partial fracture and distortion and even scale flaking and interior structural distortion of fiber. The damages occur quite randomly, but complexity and severity of damages increased with increase in drying cycles. XRD analysis showed that drying had little effect on fiber crystallinity, indicating that drying only leads to changes in the physical properties of wool fabrics instead of changes in the chemical composition of the fiber. And found SEM analysis is not only an effective method to characterize the change in the surface morphology of the fibers resulting from the drying treatments, but also explain the cause of fabric performance degradation such as pilling and strength reduction during drying. This study experimentally evaluated effects of the drying cycles on pilling propensity, mechanical and microscopic properties of wool fabrics, investigated damage models and mechanism of fabric drying in domestic.