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1

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Cyanobacterial diversity in Salar de Huasco, a high altitude saline wetland in Northern Chile, are highly similar to Antarctic cyanobacteria (2008)

Dorador, C. ; Vila, I. ; Witzel, K.-P. ; [et al.]

Oxford University Press

In: FEMS Microbiology Ecology, 64 . pp. 419-432.

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Publication Date: 2019-09-23

Description: The diversity of Cyanobacteria in water and sediment samples from four representative sites of the Salar de Huasco was examined using denaturing gradient gel electrophoresis and analysis of clone libraries of 16S rRNA gene PCR products. Salar de Huasco is a high altitude (3800 m altitude) saline wetland located in the Chilean Altiplano. We analyzed samples from a tributary stream (H0) and three shallow lagoons (H1, H4, H6) that contrasted in their physicochemical conditions and associated biota. Seventy-eight phylotypes were identified in a total of 268 clonal sequences deriving from seven clone libraries of water and sediment samples. Oscillatoriales were frequently found in water samples from sites H0, H1 and H4 and in sediment samples from sites H1 and H4. Pleurocapsales were found only at site H0, while Chroococcales were recovered from sediment samples of sites H0 and H1, and from water samples of site H1. Nostocales were found in sediment samples from sites H1 and H4, and water samples from site H1 and were largely represented by sequences highly similar to Nodularia spumigena. We suggest that cyanobacterial communities from Salar de Huasco are unique - they include sequences related to others previously described from the Antarctic, along with others from diverse, but less extreme environments.

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2

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Transport of freshened waters and ice from the Siberian shelf seas into the Arctic Basin (in Russian) (2008)

Kochetov, S. V. ; Sokolova, V. E. ; Timokhov, Leonid A.

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 92-102.

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Publication Date: 2015-04-22

Type: Book chapter , PeerReviewed

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3

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Freshwater balance of the Arctic seas (in Russian) (2008)

Kochetov, S. V. ; Timokhov, Leonid A. ; Ivanov, V. V. ; [et al.]

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 59-91.

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Publication Date: 2015-04-22

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4

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Freshening of the active water layer of the Arctic Basin and the deep-water part of the Arctic marginal seas (in Russian) (2008)

Koltyshev, A. E. ; Nikiforov, E. G. ; TImokhov, Leonid A.

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 18-28.

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Publication Date: 2015-12-10

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5

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Large-scale changes in the distribution of freshened waters in the Arctic Basin (in Russian) (2008)

Koltyshev, A. E. ; Nikiforov, E. G. ; TImokhov, Leonid A. ; [et al.]

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 37-58.

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Publication Date: 2015-04-22

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6

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Special database on temperature and salinity of the waters of the Arctic Basin and the adjoining Arctic seas in winter (in Russian) (2008)

Lebedev, N. V. ; Karpiy, V. Y. ; Pokrovsky, O. M. ; [et al.]

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 5-17.

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Publication Date: 2015-04-22

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7

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A model of vertical profiles (estimation of the thickness of the upper mixed water layer according to observation data from standard depths) (in Russian) (2008)

Lebedev, N. V. ; Timokhov, Leonid A. ; Nitishinsky, M. A.

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 242-247.

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Publication Date: 2015-04-22

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8

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Distribution of phosphates and nitrates in the region of the Laptev Sea continental slope in summer according to the data from the international expedition AVLAP/NABOS 2003 (in Russian) (2008)

Makhotin, M. S. ; Nitishinsky, M. A.

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 217-235.

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Publication Date: 2015-04-22

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9

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Major salinity anomalies and the dynamics of the freshwater reservoir of the Arctic Basin in winter (in Russian) (2008)

Nikiforov, E. G. ; Timokhov, Leonid A. ; Koltyshev, A. E. ; [et al.]

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 29-36.

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Publication Date: 2015-04-22

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10

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Surface circulation of water and ice (in Russian) (2008)

Ipatov, A. Y. ; Kulakov, I. Y. ; Timokhov, Leonid A.

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 259-266.

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Publication Date: 2015-05-18

Type: Book chapter , PeerReviewed

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11

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The upper mixed water layer in the Arctic Basin (in Russian) (2008)

Garmanov, A. L. ; Koltyshev, A. E. ; Nikiforov, E. G. ; [et al.]

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 149-189.

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Publication Date: 2015-05-18

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12

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Comment on "Phytoplankton Calcification in a High-CO2 World" (2008)

Riebesell, Ulf ; Bellerby, R. ; Engel, Anja ; [et al.]

American Association for the Advancement of Science (AAAS)

In: Science, 322 (1466b).

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Publication Date: 2019-09-23

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13

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Thermohaline intrusions in the northern Laptev Sea (in Russian) (2008)

Kirillov, Sergey A. ; Timokhov, Leonid A. ; Dmitrenko, Igor A. ; [et al.]

Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute)

In: In: Raspresnennye vody i presnyy rezerv Severnogo Ledovitogo okeana (Freshened waters and freshwater reserve of the Arctic Ocean, in Russian). Trudy AANII (Proceedings of AARI), 448 . Arkticheskiy i Antarkticheskiy Nauchno-Issledovatel'skiy Institut (Arctic and Antarctic Research Institute), St. Peterburg, Rossiya, pp. 205-216.

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Publication Date: 2015-04-22

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14

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Comparative analysis of ammonia monooxygenase (amoA) genes in the water column and sediment-water interface of two lakes and the Baltic Sea (2008)

Kim, Ok-Sun ; Junier, P. ; Imhoff, Johannes F. ; [et al.]

Oxford University Press

In: FEMS Microbiology Ecology, 66 . pp. 367-378.

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Publication Date: 2019-09-24

Description: The functional gene amoA was used to compare the diversity of ammonia-oxidizing bacteria (AOB) in the water column and sediment-water interface of the two freshwater lakes Plusssee and Schöhsee and the Baltic Sea. Nested amplifications were used to increase the sensitivity of amoA detection, and to amplify a 789-bp fragment from which clone libraries were prepared. The larger part of the sequences was only distantly related to any of the cultured AOB and is considered to represent new clusters of AOB within the Nitrosomonas/Nitrosospira group. Almost all sequences from the water column of the Baltic Sea and from 1-m depth of Schöhsee were related to different Nitrosospira clusters 0 and 2, respectively. The majority of sequences from Plusssee and Schöhsee were associated with sequences from Chesapeake Bay, from a previous study of Plusssee and from rice roots in Nitrosospira-like cluster A, which lacks sequences from Baltic Sea. Two groups of sequences from Baltic Sea sediment were related to clonal sequences from other brackish/marine habitats in the purely environmental Nitrosospira-like cluster B and the Nitrosomonas-like cluster. This confirms previous results from 16S rRNA gene libraries that indicated the existence of hitherto uncultivated AOB in lake and Baltic Sea samples, and showed a differential distribution of AOB along the water column and sediment of these environments

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15

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Expanding Oxygen-Minimum Zones in the Tropical Oceans (2008)

Stramma, Lothar ; Johnson, Gregory C. ; Sprintall, Janet ; [et al.]

American Association for the Advancement of Science (AAAS)

In: Science, 320 . pp. 655-658.

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Publication Date: 2019-09-24

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16

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Phylogenetic analysis of bacteria associated with Laminaria saccharina. (2008)

Staufenberger, Tim ; Thiel, Vera ; Wiese, Jutta ; [et al.]

Oxford University Press

In: FEMS Microbiology Ecology, 64 . pp. 65-77.

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Publication Date: 2019-09-23

Description: Bacterial communities associated with the brown alga Laminaria saccharina from the Baltic Sea and from the North Sea were investigated using denaturing gradient gel electrophoresis and 16S rRNA gene clone libraries. The rhizoid, cauloid, meristem and phyloid revealed different 16S rRNA gene denaturing gradient gel electrophoresis banding patterns indicating a specific association of bacterial communities with different parts of the alga. Associations with cauloid and meristem were more specific, while less specific associations were obtained from the old phyloid. In addition, seasonal and geographical differences in the associated communities were observed. Results from 16S rRNA gene libraries supported these findings. Bacterial phylotypes associated with the alga were affiliated with the Alphaproteobacteria (nine phylotypes), Gammaproteobacteria (nine phylotypes) and the Bacteroidetes group (four phylotypes). A number of bacteria associated with other algae and other marine macroorganisms were among the closest relatives of phylotypes associated with L. saccharina.

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17

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Impacts of atmospheric anthropogenic nitrogen on the open ocean (2008)

Duce, R.A. ; LaRoche, Julie ; Altieri, K. ; [et al.]

American Association for the Advancement of Science (AAAS)

In: Science, 320 . pp. 893-897.

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Publication Date: 2016-09-08

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18

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Comparative in silico analysis of PCR primers suited for diagnostics and cloning of ammonia monooxygenase genes from ammonia-oxidizing bacteria (2008)

Junier, P. ; Kim, O.-S. ; Molina, V. ; [et al.]

Oxford University Press

In: FEMS Microbiology Ecology, 64 . pp. 141-152.

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Publication Date: 2019-09-23

Description: Over recent years, several PCR primers have been described to amplify genes encoding the structural subunits of ammonia monooxygenase (AMO) from ammonia-oxidizing bacteria (AOB). Most of them target amoA, while amoB and amoC have been neglected so far. This study compared the nucleotide sequence of 33 primers that have been used to amplify different regions of the amoCAB operon with alignments of all available sequences in public databases. The advantages and disadvantages of these primers are discussed based on the original description and the spectrum of matching sequences obtained. Additionally, new primers to amplify the almost complete amoCAB operon of AOB belonging to Betaproteobacteria (betaproteobacterial AOB), a primer pair for DGGE analysis of amoA and specific primers for gammaproteobacterial AOB, are also described. The specificity of these new primers was also evaluated using the databases of the sequences created during this study.

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