ALBERT

Description: The aim of this study was to examine racial differences in long-term survival among hemodialysis patients after coronary artery bypass grafting (CABG). To our knowledge this has not been previously addressed in the literature. Black and white hemodialysis patients undergoing first-time, isolated CABG procedures between 1992 and 2011 were compared. Survival probabilities were computed using the Kaplan-Meier product-limit method and stratified by race. Hazard ratios (HR) and 95% confidence intervals (CI) were computed using a Cox regression model. A total of 207 (2%) patients were on hemodialysis at the time of CABG. White (n = 80) hemodialysis patients had significantly decreased 5-year survival compared with black (n = 127) patients (adjusted HR = 1.9, 95% CI = 1.2–2.8). Our finding provides useful outcome information for surgeons, primary care providers, and their patients.

Description: Background: Limited by data availability, most disease maps in the literature are for relatively large and subjectively-defined areal units, which are subject to problems associated with polygon maps. High resolution maps based on objective spatial units are needed to more precisely detect associations between disease and environmental factors. Method: We propose to use a Restricted and Controlled Monte Carlo (RCMC) process to disaggregate polygon-level location data to achieve mapping aggregate data at an approximated individual level. RCMC assigns a random point location to a polygon-level location, in which the randomization is restricted by the polygon and controlled by the background (e.g., population at risk). RCMC allows analytical processes designed for individual data to be applied, and generates high-resolution raster maps. Results: We applied RCMC to the town-level birth defect data for New Hampshire and generated raster maps at the resolution of 100 m. Besides the map of significance of birth defect risk represented by p-value, the output also includes a map of spatial uncertainty and a map of hot spots. Conclusions: RCMC is an effective method to disaggregate aggregate data. An RCMC-based disease mapping maximizes the use of available spatial information, and explicitly estimates the spatial uncertainty resulting from aggregation.

Description: The predominant microorganisms in samples taken from shower heads in residences in the Korean city “N” were Stenotrophomonas maltophilia, Sphingomonas paucimobilis, Acidovorax temperans, and Microbacterium lacticum. Legionella was not detected in this case. The volatile organic compounds (VOCs) vinylacetate, NN-DMA, cis-1,2-dichloroethylene, epichlorohydrin, and styrene were measured in five types of plastic pipes: PVC, PB, PP, PE, and cPVC. The rate of multiplication of the heterotrophic plate count (HPC) attached on the copper pipe in contact with hot tap water was higher than the rate for the copper pipe in contact with cold tap water. Biofilm accumulation on stainless steel pipes with added acetate (3 mg/L) was 2.56 times higher than the non-supplemented condition. Therefore, the growth of HPC in the pipe system was affected by the type and availability of nutrients and depended on variables such as heating during the hot water supply.

Description: Land use change as conversion pasture to forest produces several changes on hydrological cycle. In this paper we analyze the effects on stream discharge of afforestation of a small watershed devoted to pasture using the HBV hydrological model. Streamflow data obtained over the first ten years after planting were employed to evaluate the capacity of HBV model to simulate hydrological behavior of catchment after afforestation. Obtained results indicate that the estimation of streamflow was accurate as reflected by statistics (R 2  = 0.90, NSC = 0.89 and PBIAS = 0.34). Afterwards, streamflow under pasture land use (if afforestation had not occurred) was simulated using hydro-meteorological data collected during the period of study and model parameters optimized previously, together with two parameters, pcorr and cevpfo , that were adjusted for pasture conditions. HBV model results indicate that afforestation produced a water yield reduction around 2000 mm (22% of total stream discharge) during the first ten years of planting growth. The differences between forest and pasture land cover are increasing in all seasons year by year. The greatest streamflow reduction was observed in wet period (autumn and winter) with 76% of total reduction. In summer, streamflow reduction represents only 3% of total, however, represents 24.7% of discharge in this season. Streamflow reduction was related to increase of rainfall interception (mainly in wet periods) and the increase of evapotranspiration by plantation in dry periods. This article is protected by copyright. All rights reserved.

Description: Air flows from the atmosphere into an unconfined aquifer when the water table falls during pumping tests. Pumping test results in unconfined aquifers may be significantly affected by low-permeability zones (LPZs) near the initial water table position because they restrict the downward movement of air. A transient, three-dimensional (3D) air-water two-phase flow model is employed to investigate numerically the effects of local heterogeneity on pumping test results in unconfined aquifers. Two cases of local heterogeneities are considered herein: a LPZ around the pumping well and on one side of the pumping well. Results show that the drawdown with the LPZ is significantly greater than that of the homogeneous aquifer. The differences in drawdown are the most significant at intermediate times and gradually diminish at later times. The LPZ significantly reduces air flow from the atmosphere to the aquifer. The pore air velocity in the LPZ is very low. The air pressure at the observation point under the LPZ when air begins to enter is significantly lower than the air pressure of the homogeneous aquifer at the same point. After that, the air pressure increases quickly and then increases slowly. The time for the air pressure to reach the atmospheric pressure is significantly longer. This article is protected by copyright. All rights reserved.

Description: Over the last half century, since logging for timber production became intensive, Borneo has lost much of its pristine tropical forests. The long-term consequences of associated decline in precipitation are evident, and might, in turn, cause much more severe deforestation. This article is protected by copyright. All rights reserved.

Description: Bone remodeling is a natural process that enables growth and maintenance of the skeleton. It involves the deposition of mineralized matrix by osteoblasts and resorption by osteoclasts. Several cancers that metastasize to bone negatively perturb the remodeling process through a series of interactions with osteoclasts, and osteoblasts. These interactions have been described as the “vicious cycle” of cancer metastasis in bone. Due to the inaccessibility of the skeletal tissue it is difficult to study this system in vivo . In contrast, standard tissue culture lacks sufficient complexity. We have developed a specialized three-dimensional culture system that permits growth of a non-vascularized, multiple-cell-layer of mineralized osteoblastic tissue from pre-osteoblasts. In this study, the essential properties of bone remodeling were created in vitro by co-culturing the mineralized collagenous osteoblastic tissue with actively resorbing osteoclasts followed by reinfusion with proliferating pre-osteoblasts. Cell-cell and cell-matrix interactions were determined by confocal microscopy as well as by assays for cell specific cytokines and growth factors. Osteoclasts, differentiated in the presence of osteoblasts, led to degradation of the collagen-rich extracellular matrix. Further addition of metastatic breast cancer cells to the co-culture mimicked the vicious cycle; i.e. there was a further reduction in osteoblastic tissue thickness, an increase in osteoclastogenesis, chemotaxis of cancer cells to osteoclasts and formation of cancer cells into large colonies. The resulting model system permits detailed study of fundamental osteobiological and osteopathological processes in a manner that will enhance development of therapeutic interventions to skeletal diseases. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Shadows in high resolution imagery create significant problems for urban land cover classification and environmental application. We first investigated whether shadows were intrinsically different and hypothetically possible to separate from each other with ground spectral measurements. Both pixel-based and object-oriented methods were used to evaluate the effects of shadow detection on QuickBird image classification and spectroradiometric restoration. In each method, shadows were detected and separated either with or without histogram thresholding, and subsequently corrected with a k-nearest neighbor algorithm and a linear correlation correction. The results showed that shadows had distinct spectroradiometric characteristics, thus, could be detected with an optimal brightness threshold and further differentiated with a scene-based near infrared ratio. The pixel-based methods generally recognized more shadow areas and with statistically higher accuracy than the object-oriented methods. The effects of the prior shadow thresholding were not statistically significant. The accuracy of the final land cover classification, after accounting for the shadow detection and separation, was significantly higher for the pixel-based methods than for the object-oriented methods, although both achieved similar accuracy for the non-shadow classes. Both radiometric restoration algorithms significantly reduced shadow areas in the original satellite images.

Description: Despite longstanding contributions to oceanography, similar use of fluorescence light detection and ranging (LiDAR) in lake settings is not routine. The potential for ship-mounted, multispectral Ultraviolet Fluorescence LiDAR (UFL) to provide rapid, high-resolution data in variably turbid and productive lake conditions are investigated here through a series of laboratory tank and field measurements carried out on Lake Balaton, Hungary. UFL data, calibrated empirically to a set of coinciding conventionally-analyzed samples, provide simultaneous estimates of three important parameters-chlorophyll a(chla), total suspended matter (TSM) and colored dissolved organic matter (CDOM). Successful UFL retrievals from both laboratory and field measurements were achieved for chla (0.01–378 mg∙m−3; R = 0.83–0.92), TSM (0.1–130 g∙m−3; R = 0.90–0.96) and CDOM (0.003–0.125 aCDOM(440); R = 0.80–0.97). Fluorescence emission at 685 nm is shown through tank measurements to display robust but distinct relationships with chla concentration for the two cultured algae species investigated (cyanobacteria, Cylindrospermopsis raciborskii, and chlorophyta, Scenedesmus armatus). The ratio between fluorescence emissions measured at 650 nm, related to the phycocyanin fluorescence maximum, to that at 685 nm is demonstrated to effectively distinguish these two species. Validation through both laboratory measurements and field measurements confirmed that site specific calibration is necessary. This study presents the first known assessment and application of ship-mounted fluorescence LiDAR in freshwater lake conditions and demonstrates the use of UFL in measuring important water quality parameters despite the more complicated hydro-optic conditions of inland waters.

Description: Accepting the concept of standardization introduced by the standardized precipitation index (SPI), similar methodologies have been developed to construct some other standardized drought indices such as the standardized precipitation evapotranspiration index (SPEI). In this study, the authors provided deep insight into the SPEI and recognized potential deficiencies/limitations in relating to the climatic water balance it used. By coupling another well-known Palmer drought severity index (PDSI), we proposed a new standardized Palmer drought index (SPDI) through a moisture departure probabilistic approach, which allows multiscalar calculation for accurate temporal and spatial comparison of the hydro-meteorological conditions of different locations. Using datasets of monthly precipitation, temperature and soil available water capacity, the moisture deficit/surplus was calculated at multiple temporal scales and a couple of techniques were adopted to adjust corresponding time series to a generalized extreme value distribution out of several candidates. Results of the historical records (1900–2012) for diverse climates by multiple indices showed that the SPDI was highly consistent and correlated with the SPEI and self-calibrated PDSI (SC-PDSI) at most analyzed time scales. Furthermore, a simple experiment of hypothetical temperature and/or precipitation change scenarios also verified the effectiveness of this newly-derived SPDI index in response to climate change impacts. Being more robust and preferable in spatial consistency and comparability as well as combining the simplicity of calculation with sufficient accounting of the physical nature of water supply and demand relating to droughts, the SPDI is promising to serve as a competent reference and alternative for drought assessment and monitoring. This article is protected by copyright. All rights reserved.

Description: Modeled hydrologic processes are represented in a set of numerical equations, the complexity of which can be measured by the total number of variables needed. A single dominant hydrologic process could control the hydrologic response of a watershed, and so the identification of the corresponding dominant variable(s) would aid in identifying a parsimonious model and in collecting more reliable data. By accounting for both model complexity and serial correlation in the variables, a model is used to identify the dominant variables for representing watershed scale streamflow, sediment transport, and phosphorus yields. Long-term water quantity and quality data was used to show that rainfall and non-linear soil water storage were the dominant variables for weekly streamflow, suspended sediment, and particulate phosphorus. Model accuracy did not consistently improve when other statistically significant variables were included. The results suggest that improved model performance may not justify the added model complexity. As such, identification of dominant variables would be the priority for developing parsimonious hydrologic models, especially at watershed scales. This article is protected by copyright. All rights reserved.

Description: Aberrant glycosylation by N -acetylgalactosaminyl transferases (GALNTs) is a well-described pathological alteration that is widespread in hereditary diseases, prominently including human cancers, familial tumoral calcinosis and hyperostosis-hyperphosphatemia. In this study, we integrated different computational tools to perform the in silico analysis of clinically significant mutations (nsSNPs/ single amino acid change) at both functional and structural levels, found in human GALNT3, GALNT8, GALNT12 and GALNT13 genes. From function and structure based insights, mutations encoding R162Q, T359K, C574G, G359D, R297W, Y396C & D313N substitutions were concordantly predicted highly deleterious for relevant GALNTs proteins. From intriguing findings, T359K- GALNT3 was simulated with high contribution for disease susceptibility (tumor calcinosis) as compared to its partner variant T272K [Ichikawa et al., 2006]. Similarly, the prediction of high damaging behavior, evolutionary conservation and structural destabilization for C574G were proposed as major contributing factors to regulate metabolic disorder underlying tumor calcinosis and hyperostosis-hyperphosphatemia syndrome. In case of R297W- GALNT12 , prediction of highly deleterious effect and disruption in ionic interactions were anticipated with reduction in enzymatic activity, associated with bilateral breast cancer and primary colorectal cancers. The second GALNT12 mutation (D303N)-known splice variant- was predicted with disease severity as a result of decrease in charge density and buried behavior neighboring the catalytic B domain. In the lack of adequate in silico data about systematic characterization of clinically significant mutations in GALNTs genes, current study can be used as a significant tool to interpret the role of GALNTs reaction chemistry in disease-association risks in body. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: Objective To investigate whether crosstalk between RUNX2 and miRNAs is involved in tooth eruption regulated by dental follicle cells(DFCs) and the possible molecular mechanism. Methods Blood samples and embedded dental follicles were collected from patients with cleidocranial dysplasia (CCD), and RUNX2 gene mutations were analyzed, then RUNX2 +/m DFCs were isolated and identified. The characteristics of RUNX2 +/m DFCs were analyzed. The differential expression of miRNAs was detected between the RUNX2 +/m DFCs and RUNX2 +/+ DFCs by microarray, and target genes were predicted by miRGen. miR-146a was chosen for further investigation, and its effects in DFCs were analyzed by transfecting its mimics and inhibitors, and expression of genes involved in tooth eruption were detected. Results A novel insertion mutation (c.309_310insTG) of RUNX2 gene was identified which had an effect on the characteristics of DFCs. Compared with the RUNX2 +/+ DFCs, there were 69 microRNAs more than 2-fold up-regulated and 54 microRNAs more than 2-fold down-regulated in the RUNX2 +/m DFCs. Among these, miR-146a decreased significantly in RUNX 2 +/m DFCs, and expression of RUNX2, CSF-1,EGFR and OPG was significantly altered when miR-146a was over-expressed or inhibited. Conclusion RUNX2 gene mutation contributes to the characteristic change of dental follicle cells, and the crosstalk between RUNX2 gene and miRNAs may be one of the key regulatory mechanisms of differentiation of dental follicle cells. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: Cancer stem cells (CSC) have a central role in driving tumor growth. Since metabolism is becoming an important diagnostic and therapeutic target, characterization of CSC line energetic properties is an emerging need. Embryonic and adult stem cells, compared to differentiated cells, exhibit a reduced mitochondrial activity and a stronger dependence on aerobic glycolysis. Here, we aimed to comparatively analyze bioenergetics features of the human osteosarcoma 3AB-OS CSC-like line, and the parental osteosarcoma MG63 cells, from which 3AB-OS cells have been previously selected. Our results suggest that 3AB-OS cells depend on glycolytic metabolism more strongly than MG63 cells. Indeed, growth in glucose shortage or in presence of galactose or pyruvate -mitochondrial specific substrates- leads to a significant reduction of their proliferation compared to MG63 cells. Accordingly, 3AB-OS cells show an increased expression of lactate dehydrogenase A (LDHA) and a larger accumulation of lactate in the culture medium. In line with these findings 3AB-OS cells as compared to MG63 cells present a reduced mitochondrial respiration, a stronger sensitivity to glucose depletion or glycolysis inhibition and a lessened sensitivity to oxidative phosphorylation inhibitors. Additionally, in contrast to MG63 cells, 3AB-OS display fragmented mitochondria, which become networked as they grow in glucose-rich medium, while almost entirely loose these structures growing in low glucose. Overall, our findings suggest that 3AB-OS CSCs energy metabolism is more similar to normal stem cells and to cancer cells characterized by a glycolytic anaerobic metabolism. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: Areca chewing is an important environmental risk factor for development of oral premalignant lesions and cancer. Epidemiological evidence indicates that areca chewing is tightly linked to oral carcinogenesis. However, the pathogenetic impacts of areca nut extract (ANE) on normal human oral keratinocytes (HOKs) are unclear and possibly involve oxidative stress via redox imbalance. Sirtuin 3 (SIRT3) is a member of the sirtuin family of proteins that play an important role in regulating cellular reactive oxygen species (ROS) production. Recent studies have confirmed that ANE and other areca ingredients can induce ROS. In this study, we examined the role of SIRT3 in the regulation of ANE-induced ROS in HOK cells. We examined HOK cell viability following treatment with various ANE concentrations. ANE-induced cytotoxicity increased in a dose-dependent manner and was approximately 48% at a concentration of 50 μg/ml after 24 h. SIRT3 expression and enzyme activity were up-regulated in HOK cells by ANE-induced oxidative stress. Additionally, we identified that SIRT3 controls the enzymatic activity of mitochondrial proteins, such as forkhead box O3a (Foxo3a) transcription factor and antioxidant-encoding gene superoxide dismutase 2 (SOD2), by deacetylation in HOK cells. Moreover, SIRT3-mediated deacetylation and activation of Foxo3a promotes nuclear localization in vivo . These findings suggest that SIRT3 is an endogenous negative regulator in response to ANE-induced oxidative stress and demonstrate an essential role for redox balance in HOK cells. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: Collagen is the most abundant structural protein in mammals and is expressed in various tissues. In recent years, sphingosine 1-phosphate receptors (S1PRs) have been proven to play an important role in the regulation of collagen expression. Our previous studies reported that S1PRs are involved in TGF-β1-induced collagen expression via up-regulating S1PR1/3 in mouse bone marrow-derived mesenchymal stem cells (BMSCs), and result in experimental mouse liver fibrogenesis. But it remains unclear whether this process happens in human bone marrow-derived mesenchymal stem cells (hMSCs). In this study, we provide evidences that S1PR1/3, but not S1PR2, negatively regulate the expression of collagen in hMSCs using cellular and molecular approaches in vitro . We find that treatment of hMSCs with TGF-β1 up-regulated collagen expression in a dose- and time-dependent manner. Meanwhile, TGF-β1 inhibited the expression of S1PR1/3, but not S1PR2, in hMSCs in a time-dependent manner. Furthermore, either selective knock-down of S1PR1 or silencing S1PR3 induced collagen α1(I) and collagen α1(III) expression in hMSCs. In contrast, inhibition of S1PR2 by siRNA had no effects on the expression of collagen. Altogether, all these findings demonstrated that collagen expression was negatively regulated by S1PR1 and S1PR3 in hMSCs. This study highlights the differences between hMSCs and mouse BMSCs, provides a new regulation mechanism for collagen expression, and points out the risk of utilizing hMSCs in clinical applications. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: Aquaculture coasts have become widely distributed in coastal zones as human activities are intensified. Due to the complexity in this type of coast, it is difficult to extract the coastline with traditional automated mapping approaches. In this paper, we present an automated method—object-based region growing integrating edge detection (OBRGIE) for the extraction of this type of coastline. In this method, a new object feature named OMI (object merging index) is proposed to separate land and sea. The OBRGIE method was applied to Landsat Thematic Mapper (TM) (pixel size 30m) and Satellite Pour l’Observation de la Terre (SPOT-5) (pixel size 10 m) images of two coastal segments with lengths of 272.7 km and 35.5 km respectively, and the accuracy of the extracted coastlines was assessed in comparison with the manually delineated coastlines. The mean and RMSE (root mean square error) are 16.0 m and 16.4 m respectively for the TM images, and 8.0 m and 8.6 m, respectively, for the SPOT-5 images, indicating that the proposed method derives coastlines with pixel accuracy. The OBRGIE method is also found to be robust to the segmentation scale parameter, and the OMI feature is much more effective than the spectral attribute in separating land and sea in aquaculture coasts. This method may provide an inexpensive means of fast coastline mapping from remotely sensed imagery with relatively fine-to-moderate spatial resolution in coastal sectors with intense human interference.

Description: A study was undertaken to assess Envisat Advanced Synthetic Aperture Radar (ASAR) ScanSAR data for quantifying forest growing stock volume (GSV) across three boreal regions with varying forest types, composition, and structure (Sweden, Central Siberia, and Québec). Estimates of GSV were obtained using hyper-temporal observations of the radar backscatter acquired by Envisat ASAR with the BIOMASAR algorithm. In total, 5.3×106 km2 were mapped with a 0.01° pixel size to obtain estimates representative for the year of 2005. Comparing the SAR-based estimates to spatially explicit datasets of GSV, generated from forest field inventory and/or Earth Observation data, revealed similar spatial distributions of GSV. Nonetheless, the weak sensitivity of C-band backscatter to forest structural parameters introduced significant uncertainty to the estimated GSV at full resolution. Further discrepancies were observed in the case of different scales of the ASAR and the reference GSV and in areas of fragmented landscapes. Aggregation to 0.1° and 0.5° was then undertaken to generate coarse scale estimates of GSV. The agreement between ASAR and the reference GSV datasets improved; the relative difference at 0.5° was consistently within a magnitude of 20–30%. The results indicate an improvement of the characterization of forest GSV in the boreal zone with respect to currently available information.

Description: Cervical carcinoma represents the paradigm of virus-induced cancers, where virtually all cervical cancers come from previous “high-risk” HPV infection. The persistent expression of the HPV viral oncoproteins E6 and E7 is responsible for the reprogramming of fundamental cellular functions in the host cell, thus generating a noticeable, yet only partially explored, imbalance in protein molecular networks and cell signaling pathways. Eighty-eight cellular factors, identified as HPV direct or surrogate targets, were chosen and monitored in a retrospective analysis for their mRNA expression in HPV-induced cervical lesions, from dysplasia to cancer. Real-time quantitative PCR (qPCR) was performed by using formalin-fixed, paraffin embedded archival samples. Gene expression analysis identified 40 genes significantly modulated in LSIL, HSIL and squamous cervical carcinoma. Interestingly, among these, the expression level of a panel of four genes, TOP2A, CTNNB1, PFKM and GSN, was able to distinguish between normal tissues and cervical carcinomas. Immunohistochemistry was also done to assess protein expression of two genes among those up-regulated during the transition between dysplasia and carcinoma, namely E2F1 and CDC25A, and their correlation with clinical parameters. Besides the possibility of significantly enhancing the use of some of these factors in diagnostic or prognostic procedures, these data clearly outline specific pathways, and thus key biological processes, altered in cervical dysplasia and carcinoma. Deeper insight on how these molecular mechanisms work may help widen the spectrum of novel innovative approaches to these virus-induced cell pathologies. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Cellular pathways are numerous and are highly integrated in function in the control of cellular systems. They collectively regulate cell division, proliferation, survival and apoptosis of cells and mutagenesis of key genes that control these pathways can initiate neoplastic transformations. Understanding these pathways is crucial to future therapeutic and preventive strategies of the disease. Ovarian cancers are of three major types; epithelial, germ-cell and stromal. However, ovarian cancers of epithelial origin, arising from the mesothelium, are the predominant form. Of the subtypes of ovarian cancer, the high-grade serous tumors are fatal, with low survival rate due to late detection and poor response to treatments. Close examination of preserved ovarian tissues and in vitro studies have provided insights into the mechanistic changes occurring in cells mediated by a few key genes. This review will focus on pathways and key genes of the pathways that are mutated or have aberrant functions in the pathology of ovarian cancer. Non-genetic mechanisms that are gaining prominence in the pathology of ovarian cancer, miRNAs and epigenetics, will also be discussed in the review. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Normal pregnancy is associated with systemic vasodilation and decreased vascular contraction, partly due to increased release of endothelium-derived vasodilator substances. Endothelin-1 (ET-1) is an endothelium-derived vasoconstrictor acting via endothelin receptor type A (ET A R) and possibly type B (ET B R) in vascular smooth muscle cells (VSMCs), with additional vasodilator effects via endothelial ET B R. However, the role of ET-1 receptor subtypes in the regulation of vascular function during pregnancy is unclear. We investigated whether the decreased vascular contraction during pregnancy reflects changes in the expression/activity of ET A R and ET B R. Contraction was measured in single aortic VSMCs isolated from virgin, mid-pregnant (mid-Preg, day 12) and late-Preg (day 19) Sprague-Dawley rats, and the mRNA expression, protein amount, tissue and cellular distribution of ET A R and ET B R were examined using RT-PCR, Western blots, immunohistochemistry and immunofluorescence. Phenylephrine (Phe, 10 −5  M), KCl (51 mM) and ET-1 (10 −6  M) caused VSMC contraction that was in late-Preg 〈 mid-Preg and virgin rats. In VSMCs treated with ET B R antagonist BQ788, ET-1 caused significant contraction that was still in late-Preg 〈 mid-Preg and virgin rats. In VSMCs treated with the ET A R antagonist BQ123, ET-1 caused a small contraction; and the ET B R agonists IRL-1620 and sarafotoxin 6c (S6c) caused similar contraction that was in late-Preg 〈 mid-Preg and virgin rats. RT-PCR revealed similar ET A R, but greater ET B R mRNA expression in pregnant vs. virgin rats. Western blots revealed similar ET A R, and greater protein amount of ET B R in endothelium-intact vessels, but reduced ET B R in endothelium-denuded vessels of pregnant vs. virgin rats. Immunohistochemistry revealed prominent ET B R staining in the intima, but reduced ET A R and ET B R in the aortic media of pregnant rats. Immunofluorescence signal for ET A R and ET B R was less in VSMCs of pregnant vs. virgin rats. The pregnancy-associated decrease in ET A R- and ET B R-mediated VSMC contraction appears to involve downregulation of ET A R and ET B R expression/activity in VSM, and may play a role in the adaptive vasodilation during pregnancy. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Neuroregenerative medicine is an ever-growing field in which regeneration of lost cells/tissues due to a neurodegenerative disease is the ultimate goal. With the scarcity of available replacement alternatives, stem cells provide an attractive source for regenerating neural tissue. While many stem cell sources exist, including: mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs), the limited cellular potency, technical difficulties, and ethical considerations associated with these make finding alternate sources a desirable goal. Periodontal ligament stem cells (PDLSCs) derived from the neural crest were induced into neural-like cells using a combination of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). Morphological changes were evident in our treated group, seen under both light microscopy and scanning electron microscopy (SEM). A statistically significant increase in the expression of neuron-specific β-tubulin III and the neural stem/progenitor cell marker nestin, along with positive immunohistochemical staining for glial fibrillary acidic protein (GFAP), demonstrated the success of our treatment in inducing both neuronal and glial phenotypes. Positive staining for synaptophysin demonstrated neural connections and electrophysiological recordings indicated that when subjected to whole cell patch clamping, our treated cells displayed inward currents conducted through voltage-gated sodium (Na + ) channels. Taken together, our results indicate the success of our treatment in inducing PDLSCs to neural-like cells. The ease of sourcing and expansion, their embryologic neural crest origin, and the lack of ethical implications in their use make PDLSCs an attractive source for use in neuroregenerative medicine. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Urotensin II (UII), a vasoactive peptide modulates renal hemodynamics. However, the physiological functions of UII in glomerular cells are unclear. In particular, whether UII alters mesangial tone remains largely unknown. The present study investigates the physiological effects of UII on intracellular Ca 2+ ([Ca 2+ ] i ) and contraction in glomerular mesangial cells (GMCs). This study also tested the hypothesis that the regulator of G-protein signaling (RGS) controls UII receptor (UTR) activity in GMCs. RT-PCR, Western immunoblotting, and immunofluorescence revealed UTR expression and localization in cultured murine GMCs. Mouse UII (mUII) stimulated [Ca 2+ ] i elevation in GMCs in the absence and presence of extracellular Ca 2+ . mUII also caused a reduction in planar GMC surface area. mUII-induced [Ca 2+ ] i elevation and contraction in GMCs were attenuated by SB 657510, a UTR antagonist, araguspongin B, an inositol 1,4,5-trisphosphate receptor antagonist, thapsigargin, a sarco/endoplasmic reticulum Ca 2+ -ATPase inhibitor, and La 3+ , a store-operated Ca 2+ channel blocker, but not nimodipine, an L-type Ca 2+ channel blocker. In situ proximity ligation assay indicated molecular proximity between endogenous RGS2 and UTR in the cells. Treatment of GMCs with mUII increased plasma membrane association of RGS2 by ∼ 2-fold. mUII also increased the interaction between RGS2 and UTR in the cells. siRNA-mediated knockdown of RGS2 in murine GMCs increased mUII-induced [Ca 2+ ] i elevation and contraction by ∼ 35 and 31%, respectively. These findings indicate that mUII induces [Ca 2+ ] i elevation and contraction in murine GMCs. Data also suggest that UTR activation stimulates RGS2 recruitment to GMC plasma membrane as a negative feedback mechanism to regulate UTR signaling. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Poly-N-acetyllactosamine (PLN) is a unique glycan composed of repeating units of the common disaccharide (Galβ1,4-GlcNAcβ1,3) n . The expression of PLN on glycoprotein core structures minimally requires enzyme activities for β1,4-galactosyltransferase (β4GalT) and β1,3-N-acetylglucosminyltransferase (β3GnT). Because β4GalTs are ubiquitous in most cells, PLN expression is generally ascribed to the tissue-specific transcription of 8 known β3GnT genes in mice. In the olfactory epithelium (OE), β3GnT2 regulates expression of extended PLN chains that are essential for axon guidance and neuronal survival. N-glycan branching and core composition, however, can also modulate the extent of PLN modification. Here we show for the first time that the β1,6-branching glycosyltransferase GCNT2 (formerly known as IGnT) is expressed at high levels specifically in the OE and other sensory ganglia. Postnatally, GCNT2 is maintained in mature olfactory neurons that coexpress β3GnT2 and PLN. This highly specific coexpression suggests that GCNT2 and β3GnT2 function cooperatively in PLN synthesis. In support of this, β3GnT2 and GCNT2 cotransfection in HEK293T cells results in high levels of PLN expression on the cell surface and on adenylyl cyclase 3, a major carrier of PLN glycans in the OE. These data clearly suggest that GCNT2 functions in vivo together with β3GnT2 to determine PLN levels in olfactory neurons by regulating β1,6-branches that promote PLN extension. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Objective The aim of this study was to evaluate the effect of artemisinin on the proliferation and apoptosis of rat vascular smooth muscle cells (VSMCs). Method Primary rat VSMCs were treated with various doses of artemisinin. Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the messenger RNA and protein expressions of proliferating cell nuclear antigen were determined by reverse-transcription polymerase chain reaction and immunohistochemistry. Apoptosis was measured using annexin V and propidium iodide double staining evaluated by flow cytometry. Protein expression of Bax, Bcl2, and cyclin-dependent kinase 4 was determined by Western blot. Results After 72 h of treatment, artemisinin significantly inhibited VSMC proliferation in a dose-dependent manner. Treatment with 1 mM artemisinin for 72 h significantly reduced the expression of proliferating cell nuclear antigen messenger RNA. On the other hand, the same treatment increased the apoptosis of VSMCs, the activation of caspase-3, the Bax protein expression, and the Bax/Bcl2 ratio. Conclusion The results suggest that artemisinin can effectively inhibit VSMC proliferation and induce VSMC apoptosis. Copyright © 2013 John Wiley & Sons, Ltd.

Description: In 2011 an unprecedented epidemic of equine encephalitis occurred in south-eastern (SE) Australia following heavy rainfall and severe flooding in the preceding 2–4 months. Less than 6% of the documented cases occurred in Queensland, prompting the question of pre-existing immunity in Queensland horses. A small-scale serological survey was conducted on horses residing in one of the severely flood-affected areas of SE-Queensland. Using a flavivirus-specific blocking-ELISA we found that 63% (39/62) of horses older than 3 years were positive for flavivirus antibodies, and of these 18% (7/38) had neutralizing antibodies to Murray Valley encephalitis virus (MVEV), Kunjin virus (WNVKUN) and/or Alfuy virus (ALFV). The remainder had serum-neutralizing antibodies to viruses in the Kokobera virus (KOKV) complex or antibodies to unknown/untested flaviviruses. Amongst eight yearlings one presented with clinical MVEV-encephalomyelitis, while another, clinically normal, had MVEV-neutralizing antibodies. The remaining six yearlings were flavivirus antibody negative. Of 19 foals born between August and November 2011 all were flavivirus antibody negative in January 2012. This suggests that horses in the area acquire over time active immunity to a range of flaviviruses. Nevertheless, the relatively infrequent seropositivity to MVEV, WNVKUN and ALFV (15%) suggests that factors other than pre-existing immunity may have contributed to the low incidence of arboviral disease in SE-Queensland horses during the 2011 epidemic.

Description: With an ever increasing population rate; a vast array of biomass wastes rich in organic and inorganic nutrients as well as pathogenic microorganisms will result from the diversified human, industrial and agricultural activities. Anaerobic digestion is applauded as one of the best ways to properly handle and manage these wastes. Animal wastes have been recognized as suitable substrates for anaerobic digestion process, a natural biological process in which complex organic materials are broken down into simpler molecules in the absence of oxygen by the concerted activities of four sets of metabolically linked microorganisms. This process occurs in an airtight chamber (biodigester) via four stages represented by hydrolytic, acidogenic, acetogenic and methanogenic microorganisms. The microbial population and structure can be identified by the combined use of culture-based, microscopic and molecular techniques. Overall, the process is affected by bio-digester design, operational factors and manure characteristics. The purpose of anaerobic digestion is the production of a renewable energy source (biogas) and an odor free nutrient-rich fertilizer. Conversely, if animal wastes are accidentally found in the environment, it can cause a drastic chain of environmental and public health complications.

Description: Scant information is available on determinants of smoking prevalence in the vulnerable population of the elderly, particularly in Europe. Therefore, we analyzed smoking patterns among older adults (≥65 years old), using data from a representative survey based on 3,071 elderly, conducted in 17 European countries in 2010, within the Pricing Policies And Control of Tobacco in Europe (PPACTE) project. Overall smoking prevalence in 17 European countries was 11.5% (15.3% in men and 8.6% in women). An inverse relation with level of education was observed among men, while no specific pattern was evident among women. Smoking prevalence was highest in eastern/central Europe for men (20.3%) and northern Europe for women (13.1%). In both sexes combined, smokers were more frequent in countries with low implementation of tobacco control activities (14.9%). Anti-tobacco campaigns and smoking cessation interventions specifically targeted to the elderly are urgently needed in Europe.

Description: The Multi-angle Imaging SpectroRadiometer (MISR) instrument aboard the Terra satellite acquires imagery at 275-m resolution at nine angles ranging from 0° (nadir) to 70° off-nadir. This multi-angle capability facilitates the stereoscopic retrieval of heights and motion vectors for clouds and aerosol plumes. MISR’s operational stereo product uses this capability to retrieve cloud heights and winds for every satellite orbit, yielding global coverage every nine days. The MISR INteractive eXplorer (MINX) visualization and analysis tool complements the operational stereo product by providing users the ability to retrieve heights and winds locally for detailed studies of smoke, dust and volcanic ash plumes, as well as clouds, at higher spatial resolution and with greater precision than is possible with the operational product or with other space-based, passive, remote sensing instruments. This ability to investigate plume geometry and dynamics is becoming increasingly important as climate and air quality studies require greater knowledge about the injection of aerosols and the location of clouds within the atmosphere. MINX incorporates features that allow users to customize their stereo retrievals for optimum results under varying aerosol and underlying surface conditions. This paper discusses the stereo retrieval algorithms and retrieval options in MINX, and provides appropriate examples to explain how the program can be used to achieve the best results.

Description: Roads play an indispensable role as part of the infrastructure of society. In recent years, society has witnessed the rapid development of laser mobile mapping systems (LMMS) which, at high measurement rates, acquire dense and accurate point cloud data. This paper presents a way to automatically estimate the required excavation volume when widening a road from point cloud data acquired by an LMMS. Firstly, the input point cloud is down-sampled to a uniform grid and outliers are removed. For each of the resulting grid points, both on and off the road, the local surface normal and 2D slope are estimated. Normals and slopes are consecutively used to separate road from off-road points which enables the estimation of the road centerline and road boundaries. In the final step, the left and right side of the road points are sliced in 1-m slices up to a distance of 4 m, perpendicular to the roadside. Determining and summing each sliced volume enables the estimation of the required excavation for a widening of the road on the left or on the right side. The procedure, including a quality analysis, is demonstrated on a stretch of a mountain road that is approximately 132 m long as sampled by a Lynx LMMS. The results in this particular case show that the required excavation volume on the left side is 8% more than that on the right side. In addition, the error in the results is assessed in two ways. First, by adding up estimated local errors, and second, by comparing results from two different datasets sampling the same piece of road both acquired by the Lynx LMMS. Results of both approaches indicate that the error in the estimated volume is below 4%. The proposed method is relatively easy to implement and runs smoothly on a desktop PC. The whole workflow of the LMMS data acquisition and subsequent volume computation can be completed in one or two days and provides road engineers with much more detail than traditional single-point surveying methods such as Total Station or GPS profiling. A drawback is that an LMMS system can only sample what is within the view of the system from the road.

Description: The Board of Parks and Recreation in Vancouver, BC approved a smoke-free bylaw in the city’s parks, beaches and recreational facilities, effective 1 September 2010. We analyzed local news coverage and portrayal of the bylaw to understand the potential influence of news media on public perception of the bylaw in order to inform the media advocacy work of public health interest groups. We compiled a data set of newspaper articles (n = 90) and conducted a quantitative content analysis to examine content related to the outdoor smoke-free policy, including article slant, topics related to smoking and tobacco control, and any equity-related concerns raised. Newspaper coverage in Vancouver was largely supportive of the outdoor smoke-free bylaw. However, concerns over rights were frequently discussed in letters to the editor. Such equity concerns were rarely discussed in news articles, showing a potential disconnect between the concerns expressed in the media by members of the public and the coverage provided by print media.

Description: At present continental to global scale flood forecasting predicts at a point discharge, with little attention to detail and accuracy of local scale inundation predictions. Yet, inundation variables are of interest and all flood impacts are inherently local in nature. This paper proposes a large scale flood inundation ensemble forecasting model that uses best available data and modeling approaches in data scarce areas. The model was built for the Lower Zambezi River to demonstrate current flood inundation forecasting capabilities in large data-scarce regions. ECMWF ensemble forecast (ENS) data were used to force the VIC (Variable Infiltration Capacity) hydrological model, which simulated and routed daily flows to the input boundary locations of a 2-D hydrodynamic model. Efficient hydrodynamic modeling over large areas still requires model grid resolutions that are typically larger than the width of channels that play a key a role in flood wave propagation. We therefore employed a novel sub-grid channel scheme to describe the river network in detail whilst representing the floodplain at an appropriate scale. The modeling system was calibrated using channel water levels from satellite laser altimetry and then applied to predict the February 2007 Mozambique floods. Model evaluation showed that simulated flood edge cells were within a distance of between one and two model resolutions compared to an observed flood edge and inundation area agreement was on average 86%. Our study highlights that physically plausible parameter values and satisfactory performance can be achieved at spatial scales ranging from tens to several hundreds of thousands of km 2 and at model grid resolutions up to several km 2 .

Description: A class of capillary flows in unsaturated porous media is characterized by quasi-steady viscous flow confined behind curved air-water interfaces and within liquid bodies held by capillary forces along crevices and grain contacts. The geometry of the connected capillary liquid network within the pore space resembles channels that form between adjacent bubbles in foam (Plateau borders) with solid grains representing gas bubbles in foam. For simplified channel geometry we combine expressions for viscous flow with continuity considerations to describe the evolution of the channels cross-sectional area during gravity drainage. This formulation enables modeling of unsaturated flow without invoking the Richards equation and associated hydraulic functions. We adapt a formalism originally developed for foam “free drainage” (drainage under gravity) or “forced drainage” (infiltration front motion) to a class of unsaturated flows in porous media that require a few input parameters only (mean channel corner angle, air entry value and porosity) for certain initial and boundary conditions. We demonstrate that the reduction in capillary channel cross section yields a consistent description of self-regulating internal fluxes towards attainment of the so-called “field capacity” in soil and provides an alternative method for interpretation of outflow experiments for prescribed pressure boundary conditions. Additionally, the geometrically-explicit formulation provides a more intuitive picture of capillary flows across textural boundaries (changes in channel cross-section and number of channels). The foam drainage methodology expands the range of tools available for analyses of unsaturated flow processes and offers more realistic links between liquid configuration and flow dynamics in unsaturated porous media.

Description: Synthetic streamflows at different sites in a river basin are needed for planning, operation and management of water resources projects. Modeling the temporal and spatial dependence structure of monthly streamflow at different sites is generally required. In this study, the maximum entropy copula method is proposed for multisite monthly streamflow simulation, in which the temporal and spatial dependence structure is imposed as constraints to derive the maximum entropy copula. The monthly streamflows at different sites are then generated by sampling from the conditional distribution. A case study for the generation of monthly streamflow at three sites in the Colorado River basin illustrates the application of the proposed method. Simulated streamflow from the maximum entropy copula is in satisfactory agreement with observed streamflow.

Description: The Krycklan Catchment Study (KCS) provides a unique field infrastructure for hillslope to landscape-scale research on short and long-term ecosystem dynamics in boreal landscapes. The site is designed for process-based research assessing the role of external drivers including forest management, climate change, and long-range pollutant transport on forests, mires, soils, streams, lakes and groundwater. The over-arching objectives of KCS are to (1) provide a state-of-the-art infrastructure for experimental and hypothesis driven research, (2) maintain a collection of high quality, long-term climatic, biogeochemical, hydrological and environmental data, and (3) support the development of models and guidelines for research, policy and management.

Description: 3-D Hydraulic Tomography (3-D HT) is a method for aquifer characterization whereby the 3-D spatial distribution of aquifer flow parameters (primarily hydraulic conductivity, K) is estimated by joint inversion of head change data from multiple partially-penetrating pumping tests. While performance of 3-D HT has been studied extensively in numerical experiments, few field studies have demonstrated the real-world performance of 3-D HT. Here we report on a 3-D transient hydraulic tomography (3-D THT) field experiment at the Boise Hydrogeophysical Research Site which is different from prior approaches in that it represents a “baseline” analysis of 3-D THT performance using only a single arrangement of a central pumping well and 5 observation wells with nearly complete pumping and observation coverage at 1m intervals. We jointly analyze all pumping tests using a geostatistical approach based on the quasi-linear estimator of kitanidis [1995]. We reanalyze the system after progressively removing pumping and/or observation intervals; significant progressive loss of information about heterogeneity is quantified as reduced variance of the K field overall, reduced correlation with slug test K estimates at wells, and reduced ability to accurately predict independent pumping tests. We verify that imaging accuracy is strongly improved by pumping and observational densities comparable to the aquifer heterogeneity geostatistical correlation lengths. Discrepancies between K profiles at wells, as obtained from HT and slug tests, are greatest at the tops and bottoms of wells where HT observation coverage was lacking.

Description: Previous studies indicate that muscle Pgc-1α expression governs the proportion of muscle fibre types. As a first step in using diet to manipulate the proportion of muscle fibre types by using Pgc-1α expression, the present study investigates the modulation of Pgc-1α expression by feedstuffs. A luciferase-based Pgc-1α reporter construct (Pgc-1α(-2553)-luc) that contains the mouse Pgc-1α promoter (−2553 to +78 bp) was prepared. A screen of ethanol extracts from 33 feedstuffs indicated that oolong tea and roasted green tea extracts decreased Pgc-1α(-2553)-luc expression in C2C12 myoblasts. The transcriptional repression of Pgc-1α by tea leaf extracts was reproduced in hepatic HepG2 cells. We further examined the effects of the alcohol extracts of tea waste and its silage on Pgc-1α transcription; the tea waste silage extract inhibited Pgc-1α transcription. Treatment with the extracts of raw tea leaves, tea waste and tea waste silage effectively decreased Pgc-1α mRNA levels during myogenesis of myosatellite cells. The present results suggest that tea leaves and their by-products could be used to modulate proportions of muscle fibre types. Copyright © 2013 John Wiley & Sons, Ltd.

Description: We applied graphical methods and multivariate statistics to understand impacts of an unsewered slum catchment on nutrients and hydrochemistry of groundwater in Kampala, Uganda. Data was collected from 56 springs (groundwater), 22 surface water sites and 13 rain samples. Groundwater was acidic and dominated by Na, Cl and NO 3 . These ions were strongly correlated indicating pollution originating from wastewater infiltration from on-site sanitation systems. Results also showed that rain, which was acidic, impacted on groundwater chemistry. Using Q-mode hierarchical cluster analysis, we identified three distinct water quality groups. The first group had springs dominated by Ca-Cl-NO 3 , low values of EC, pH and cations, and relatively high NO 3 values. These springs were shown to have originated from the acidic rains because their chemistry closely corresponded to ion concentrations that would occur from rainfall recharge, which was around 3.3 times concentrated by evaporation. The second group had springs dominated by Na-K-Cl-NO 3 and Ca-Cl-NO 3 , low pH but with higher values of EC, NO 3 and cations. We interpreted these as groundwater affected by both acid rain and infiltration of wastewater from urban areas. The third group had the highest EC values (average of 688 μS/cm), low pH and very high concentrations of NO 3 (average of 2.15 mmol/L) and cations. These springs were exclusively located in slum areas and we interpreted these springs as groundwater affected by infiltration of wastewater from poorly sanitized slums areas. Surface water was slightly reducing and eutrophic due to wastewater effluents, but the contribution of groundwater to nutrients in surface water was minimal because o-PO 4 was absent whereas NO 3 was lost by denitification. Our findings suggest that groundwater chemistry in the catchment is strongly influenced by anthropogenic inputs derived from nitrogen-containing rains and domestic wastewater. This article is protected by copyright. All rights reserved.

Description: Lymph nodes are often the first target of metastatic cancer which can then remetastasize to distant organs. The progression of lymph node metastasis is dependent on sufficient blood supply provided by angiogenesis. In the present study, we have developed a color-coded imaging model to visualize angiogenesis of lymph nodes metastasis using green fluorescent protein (GFP) and red fluorescent protein (RFP). Transgenic mice carrying GFP under the control of the nestin second-intron enhancer (ND-GFP mice) were used as hosts. Nascent blood vessels express GFP in these mice. B16F10-RFP melanoma cells were injected into the efferent lymph vessel of the inguinal lymph node of the ND-GFP nude mice, whereby the melanoma cells trafficked to the axillary lymph node. Three days after melanoma implantation, ND-GFP-expressing nascent blood vessels were imaged in the axillary lymph nodes. Seven days after implantation, ND-GFP-expressing nascent blood vessels formed a network in the lymph nodes. ND-GFP-positive blood vessels surrounded the tumor mass by 14 days after implantation. However, by 28 days after implantation, ND-GFP expression was diminished as the blood vessels matured. Treatment with doxorubicin significantly decreased the mean nascent blood vessel length per tumor volume. These results show that the dual-color ND-GFP blood vessels/RFP-tumor model is a powerful tool to visualize and quantitate angiogenesis of metastatic lymph nodes as well as for evaluation of its inhibition. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: Spermatogenesis is a special process by which spermatogonial stem cells (SSCs) divide and differentiate to male gametes called mature spermatozoa. SSCs are the unique cells because they are adult stem cells that transmit genetic information to subsequent generations. Accumulating evidence has demonstrated that SSCs can be reprogrammed to acquire pluripotency to become embryonic stem-like cells that differentiate into all cell lineages of the three germ layers, highlighting potential important applications of SSCs for regenerative medicine. Recent studies from peers and us have made great achievements on the characterization, isolation and culture of mouse and human SSCs, which could lead to better understanding the biology of SSCs and the applications of SSCs in both reproductive and regenerative medicine. In this review, we first compared the cell identity and biochemical phenotypes between mouse SSCs and human SSCs. Notably, the cell types of mouse and human SSCs are distinct, and human SSCs share some but not all phenotypes with mouse SSCs. The approaches for isolating SSCs as well as short- and long- term culture of mouse SSCs and short-period culture of human SSCs were also discussed. We further addressed the new advances on the self-renewal of SSCs with an aim to establish the long-term culture of human SSCs which has not yet been achieved. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: Probabilistic estimates of future water levels and river discharge are usually simulated with hydrologic models using ensemble weather forecasts as main inputs. As hydrologic models are imperfect and the meteorological ensembles tend to be biased and underdispersed, the ensemble forecasts for river runoff typically are biased and underdispersed, too. Thus, in order to achieve both reliable and sharp predictions statistical post-processing is required. In this work Bayesian model averaging (BMA) is applied to statistically post-process ensemble runoff raw forecasts for a catchment in Switzerland, at lead-times ranging from 1 to 240 hours. The raw forecasts have been obtained using deterministic and ensemble forcing meteorological models with different forecast lead-time ranges. First, BMA is applied based on mixtures of univariate normal distributions, subject to the assumption of independence between distinct lead-times. Then, the independence assumption is relaxed in order to estimate multivariate runoff forecasts over the entire range of lead-times simultaneously, based on a BMA version that uses multivariate normal distributions. Since river runoff is a highly skewed variable, Box-Cox transformations are applied in order to achieve approximate normality. Both univariate and multivariate BMA approaches are able to generate well calibrated probabilistic forecasts that are considerably sharper than climatological forecasts. Additionally, multivariate BMA provides a promising approach for incorporating temporal dependencies into the post-processed forecasts. Its major advantage against univariate BMA is an increase in reliability when the forecast system is changing due to model availability.

Description: We derive a series solution for the nonlinear Boussinesq equation in terms of the similarity variable of the Boltzmann transformation in a semi-infinite domain. The first few coefficients of the series have been known for a long time, having been obtained by a truncated inversion of the series solution of the Blasius equation, but no direct recurrence relation was known for the complete series representing the solution of the Boussinesq equation. The series turns out to have a finite radius of convergence, which we estimate with a numerical complex-plane integration method that identifies the singularities of the solution when the equation is extended to the complex plane. The homogeneous condition at the origin produces a singularity which complicates numerical solutions with Runge-Kutta methods. We present two variable transformations that circumvent the problem and that are best suited to the complex-variable and the real-variable versions of the equation, respectively. Using those tools, an approximate solution accurate to 1.75 × 10 -10 and valid for the entire positive real axis is then developed by matching a Padé approximant of the exact series and an asymptotic solution (to overcome the restriction imposed by the finite radius of convergence of the series), along the same lines of the expression proposed by Hogarth and Parlange [1999]. The accuracies of all of the existing and the newly proposed solutions are obtained.

Description: Field sampling in unwadeable and flashy flood events encounters the problem that lateral variability of flow hydraulics and sediment transport cannot be captured adequately, and there is also an accuracy problem because parameters change while being measured. Moreover, event based gravel-sand mixed transport data in rapidly changing conditions are largely missing, in particular for gravel-bed rivers in small catchments. In this study, field measurements of bed load, suspended load, flow velocities, water depths and cross section geometry were collected during flood events at a monitoring station near the mouth of the Versilia river, Italy. Since the observed hydrographs are characterized by short durations, to the order of a few hours, an analysis of the lateral and temporal flow variability was carried out to enable the design of a sampling strategy and to minimize the errors created by the time variations of discharge associated with unsteady flow conditions. The measurements were interpreted using a 1D hydro-morphodynamic numerical model simulating the dynamics of flow and sediment discharges during a flood event for a given return period. The flow and sediment rating curves were then developed through an integrated approach combining different methodologies: field measurements, laboratory analyses and mathematical modeling. The developed approach allows one to capture the main physical mechanisms associated to the transport of sand–gravel mixtures, such as selective transport, and the hysteretic behaviour of sediment transport produced by rapid and intense flood events.

Description: Long non-coding RNAs (lncRNAs) have recently gained increasing attention because of their crucial roles in gene regulatory processes. Functional studies using mammalian skin as a model system have revealed their role in controlling normal tissue homeostasis as well as the transition to a diseased state. Here, we describe how lncRNAs regulate differentiation to preserve an undifferentiated epidermal progenitor compartment, and to maintain a functional skin permeability barrier. Furthermore, we will reflect on recent work analyzing the impact of lncRNAs on the progression from normal epithelium to the development of skin disorders and cancer. Long non-coding RNAs (lncRNAs) have recently been shown to control a wide variety of gene regulatory processes. In mammalian skin, lncRNAs appear to regulate the intricate balance between progenitor cells undergoing continual regeneration in the basal layer and highly differentiated cells forming the epidermal permeability barrier.

Description: This study examined factors associated with point-of-sale tobacco marketing in St. Louis, an urban city in the United States. Using spatial analysis, descriptive statistics, and multilevel modeling, we examined point-of-sale data and the proportion of mentholated cigarette and total cigarette marketing from 342 individual tobacco retail stores within St. Louis census tracts characterized by the percent of black adults and children. Menthol and total tobacco product marketing was highest in areas with the highest percentages of black residents. When examining menthol marketing to children, we did not find as strong of a relationship, however results of multilevel modeling indicate that as the proportion of black children in a census tract increased, the proportion of menthol marketing near candy also increased. These results indicate the need for communities globally to counter this targeted marketing by taking policy action specifically through the enactment of marketing restrictions provided by the 2009 Family Smoking Prevention and Tobacco Control Act and the Framework Convention of Tobacco Control.

Description: Hepatitis E virus (HEV) is responsible for epidemics and endemics of acute hepatitis in humans, mainly through waterborne, foodborne, and zoonotic transmission routes. HEV is a single-stranded, positive-sense RNA virus classified in the family Hepeviridae and encompasses four known Genotypes (1–4), at least two new putative genotypes of mammalian HEV, and one floating genus of avian HEV. Genotypes 1 and 2 HEVs only affect humans, while Genotypes 3 and 4 are zoonotic and responsible for sporadic and autochthonous infections in both humans and several other animal species worldwide. HEV has an ever-expanding host range and has been identified in numerous animal species. Swine serve as a reservoir species for HEV transmission to humans; however, it is likely that other animal species may also act as reservoirs. HEV poses an important public health concern with cases of the disease definitively linked to handling of infected pigs, consumption of raw and undercooked animal meats, and animal manure contamination of drinking or irrigation water. Infectious HEV has been identified in numerous sources of concern including animal feces, sewage water, inadequately-treated water, contaminated shellfish and produce, as well as animal meats. Many aspects of HEV pathogenesis, replication, and immunological responses remain unknown, as HEV is an extremely understudied but important human pathogen. This article reviews the current understanding of HEV transmission routes with emphasis on food and environmental sources and the prevalence of HEV in animal species with zoonotic potential in humans.

Description: The slow movement of active deep-seated slope gravitational deformations (DSGSDs) and deep-seated rockslides can cause damage to structures and infrastructures. We use Permanent Scatterers Synthetic Aperture Radar Interferometry (PSInSAR™) displacement rate data for the analysis of DSGSD/rockslide activity and kinematics and for the analysis of damage to buildings. We surveyed the degree of damage to buildings directly in the field, and we tried to correlate it with the superficial displacement rate obtained by the PSInSAR™ technique at seven sites. Overall, we observe that the degree of damage increases with increasing displacement rate, but this trend shows a large dispersion that can be due to different causes, including: the uncertainty in the attribution of the degree of damage for buildings presenting wall coatings; the complexity of the deformation for large phenomena with different materials and subjected to differential behavior within the displaced mass; the absence of differential superficial movements in buildings, due to the large size of the investigated phenomena; and the different types of buildings and their position along the slope or relative to landslide portions.

Description: To understand the impact of drinking and driving laws on drinking and driving fatality rates, this study explored the different effects these laws have on areas with varying severity rates for drinking and driving. Unlike previous studies, this study employed quantile regression analysis. Empirical results showed that policies based on local conditions must be used to effectively reduce drinking and driving fatality rates; that is, different measures should be adopted to target the specific conditions in various regions. For areas with low fatality rates (low quantiles), people’s habits and attitudes toward alcohol should be emphasized instead of transportation safety laws because “preemptive regulations” are more effective. For areas with high fatality rates (or high quantiles), “ex-post regulations” are more effective, and impact these areas approximately 0.01% to 0.05% more than they do areas with low fatality rates.

Description: The Chinese government enforced mandatory quarantine for 60 days (from 10 May to 8 July 2009) as a preventative strategy to control the spread of the 2009 H1N1 pandemic. Such a prevention strategy was stricter than other non-pharmaceutical interventions that were carried out in many other countries. We evaluated the effectiveness of the mandatory quarantine and provide suggestions for interventions against possible future influenza pandemics. We selected one city, Beijing, as the analysis target. We reviewed the epidemiologic dynamics of the 2009 H1N1 pandemic and the implementation of quarantine measures in Beijing. The infectious population was simulated under two scenarios (quarantined and not quarantined) using a deterministic Susceptible-Exposed-Infectious-Recovered (SEIR) model. The basic reproduction number R0 was adjusted to match the epidemic wave in Beijing. We found that mandatory quarantine served to postpone the spread of the 2009 H1N1 pandemic in Beijing by one and a half months. If mandatory quarantine was not enforced in Beijing, the infectious population could have reached 1,553 by 21 October, i.e., 5.6 times higher than the observed number. When the cost of quarantine is taken into account, mandatory quarantine was not an economically effective intervention approach against the 2009 H1N1 pandemic. We suggest adopting mitigation methods for an influenza pandemic with low mortality and morbidity.

Description: This study employed a qualitative approach to explore the attitudes and experiences of older people towards using gerontechnology, and to determine the underlying reasons that might account for their use and non-use of gerontechnology. Four focus group discussions and 26 individual interviews were undertaken. Qualitative data were analyzed using NVivo software and were categorized using coding and grounded theory techniques. The result indicated that old people in Hong Kong had an overall positive attitude toward technology. Positive attitudes were most frequently related to enhanced convenience and advanced features. Negative attitudes were most frequently associated with health risks and social problems arising from using technology (e.g., social isolation and addiction). Usage of technology is driven by outcome expectations and social influences, and supported by facilitators, whereas non-use of gerontechnology relates to the personal (e.g., health and functional capacities), technological (e.g., cost and complexity), and environmental barriers experienced. Use of gerontechnology is a synthesis of person, technology, and environment. To encourage non-users to adopt technology, there is a need to remove barriers at personal, technological, and environmental levels.

Description: The authors wish to add the following amendments and corrections to their paper published in IJERPH [1].

Description: Entomological, veterinary, and human surveillance systems for West Nile virus (WNV) infection have been implemented in Italy since the first detection of the virus in 1998. These surveillance activities documented a progressive increase of WNV activity and spread in different regions and the emergence of new WNV lineages and strains. Italy is a paradigmatic example of the complex epidemiology of WNV in Europe, where sporadic cases of WNV infection, clusters, and small outbreaks have been reported in several regions. In addition, different strains of both WNV lineage 1 and lineage 2 have been identified, even co-circulating in the same area.

Description: A 30-year series of global monthly Normalized Difference Vegetation Index (NDVI) imagery derived from the Global Inventory Modeling and Mapping Studies (GIMMS) NDVI3g archive was analyzed for the presence of trends in changing seasonality. Using the Seasonal Trend Analysis (STA) procedure, over half (56.30%) of land surfaces were found to exhibit significant trends. Almost half (46.10%) of the significant trends belonged to three classes of seasonal trends (or changes). Class 1 consisted of areas that experienced a uniform increase in NDVI throughout the year, and was primarily associated with forested areas, particularly broadleaf forests. Class 2 consisted of areas experiencing an increase in the amplitude of the annual seasonal signal whereby increases in NDVI in the green season were balanced by decreases in the brown season. These areas were found primarily in grassland and shrubland regions. Class 3 was found primarily in the Taiga and Tundra biomes and exhibited increases in the annual summer peak in NDVI. While no single attribution of cause could be determined for each of these classes, it was evident that they are primarily found in natural areas (as opposed to anthropogenic land cover conversions) and that they are consistent with climate-related ameliorations of growing conditions during the study period.

Description: We examined the water balance a forested ombrotrophic peatland and adjacent burned peatland in the boreal plain of western Canada over a three-year period. Complete combustion of foliage and fine branches dramatically increased shortwave radiation inputs to the peat surface while halting all tree transpiration at the burned site. End-of-winter snowpack was 7-25% higher at the burned site likely due to decreased ablation from the tree canopy at the unburned site. Shrub regrowth at the burned site was rapid post-fire, and shading by the shrub canopy in the burned site approached that of the unburned site within three years after fire. Site-averaged surface resistance to evaporation was not different between sites, though surface resistance in hollows was lower in the burned site. Water loss at both burned and unburned sites is largely driven by surface evaporative losses. Evaporation at the burned site marginally exceeded the sum of pre-fire transpiration and interception at the unburned site, suggesting that ET during the growing season was 2 0–40  mm greater at the the burned peatland. While the net change in water storage during the growing season was largely unchanged by fire, the lack of low-density surface peat in the burned site appears to have decreased specific yield, leading to greater water table decline at the burned site despite similar net change in storage. This article is protected by copyright. All rights reserved.

Description: The eddy covariance (EC) method was used in a 30-month study to quantify evapotranspiration (ET) and vegetation coefficient (K CW ) for a wetland on a ranch in subtropical south Florida. To evaluate the errors in ET estimates, the EC-based ET (ET C.-EC ) and the Food and Agricultural Organization (FAO) Penman-Monteith (PM) based ET (ET C.-PM ) estimates (with literature crop coefficient K C ) were compared to each other. The ET C.-EC and FAO-PM reference ET were used to develop K CW . Regression models were developed to estimate K CW using climatic and hydrologic variables. Annual and daily ET C.-EC values were 1152 mm and 3.27 mm, respectively. The FAO-PM model underestimated ET by 25% with ET C.-EC being statistically higher than ET C.-PM . The K CW varied from 0.79 (December) to 1.06 (November). The mean K CW for dry (November-April) season (0.95) was much higher than values reported for wetlands in literature, while wet (May-October) season K CW (0.97) was closer to literature values. Higher than expected K CW values during dry season were due to higher temperature, lower humidity and perennial wetland vegetation. Regression analyses showed that factors affecting the K CW were different during the dry (soil moisture, temperature, and relative humidity) and wet (net radiation, inundation, and wind speed) seasons. Separate regression models for the dry and wet seasons were developed. ET and K CW from this study, one of the first for the agricultural wetlands in subtropical environment, will help improve the ET estimates for similar wetlands. This article is protected by copyright. All rights reserved.

Description: Research on runoff processes to date has focused on the differences between the main divisions of runoff partitioning. Indeed, our major advancements in runoff theory have come with new differentiations of different forms of overland flow and subsurface stormflow. These studies of ‘how runoff processes are different’ have resulted in our current summaries of runoff regimes conceptually (e.g. the Variable Source Area (VSA) concept) and codified in our models (e.g. TOPMODEL and its derivatives). This article is protected by copyright. All rights reserved.

Description: Daily pollution data (collected in Graz over 16 years and in the Linz over 18 years) were used for time series studies (GAM and case-crossover) on the relationship with daily mortality (overall and specific causes of death). Diagnoses of patients who had been transported to hospitals in Linz were also available on a daily basis from eight years for time series analyses of cardiopulmonary emergencies. Increases in air pollutant levels over several days were followed by increases in mortality and the observed effects increased with the length of the exposure window considered, up to a maximum of 15 days. These mortality changes in Graz and Linz showed similar patterns like the ones found before in Vienna. A significant association of mortality could be demonstrated with NO2, PM2.5 and PM10 even in summer, when concentrations are lower and mainly related to motor traffic. Cardiorespiratory ambulance transports increased with NO2/PM2.5/PM10 by 2.0/6.1/1.7% per 10 µg/m³ on the same day. Monitoring of NO2 (related to motor traffic) and fine particulates at urban background stations predicts acute effects on cardiopulmonary emergencies and extended effects on cardiopulmonary mortality. Both components of urban air pollution are indicators of acute cardiopulmonary health risks, which need to be monitored and reduced, even below current standards.

Description: ABSTRACT A study was performed to characterize over land precipitation associated with tropical cyclones (TCs) for basins around the world based upon the International Best Track Archive for Climate Stewardship (IBTrACS). From 1998 to 2009, data from the Tropical Rainfall Measuring Mission (TRMM) Multi-satellite Precipitation Analysis (TMPA) product 3B42, showed that TCs accounted for 5.5%, 7.5%, 6%, 9.5%, and 8.9% of the annual precipitation for impacted over land areas of the Americas, East Asia, South and West Asia, Oceania, and East Africa respectively, and that TC contribution decreased significantly within the first 150-km from the coast. Locally, TCs contributed on average to more than 25% and up to 61% of the annual precipitation budget over very different climatic areas with arid or tropical characteristics. East Asia represented the higher and most constant TC rain (118±19% mm y -1 ) normalized over the area impacted, while East Africa presented the highest variability (108±60% mm y -1 ), and the Americas displayed the lowest average TC rain (65±24% mm y -1 ) despite a higher TC activity. Furthermore, the maximum monthly TC contribution (8-11%) was found later in the TC season and depended on the peak of TC activity, TC rainfall, and the domain transition between dry and wet regimes if any. Finally, because of their importance in terms of rainfall amount, the contribution of TCs was provided for a selection of 50 urban areas experiencing cyclonic activity. Results showed that for particularly intense years, urban areas prone to cyclonic activity received more than half of their annual rainfall from TCs.

Description: A multivariate, multi-site daily weather generator is presented for use in decision-centric vulnerability assessments under climate change. The tool is envisioned useful for a wide range of socioeconomic and biophysical systems sensitive to different aspects of climate variability and change. The proposed stochastic model has several components, including 1) a wavelet decomposition coupled to an autoregressive model to account for structured, low-frequency climate oscillations, 2) a Markov Chain and k-nearest-neighbor (KNN) resampling scheme to simulate spatially-distributed, multivariate weather variables over a region, and 3) a quantile mapping procedure to enforce long-term distributional shifts in weather variables that result from prescribed climate changes. The Markov Chain is used to better represent wet and dry spell statistics while the KNN bootstrap resampler preserves the covariance structure between the weather variables and across space. The wavelet-based autoregressive model is applied to annual climate over the region and used to modulate the Markov Chain and KNN resampling, embedding appropriate low-frequency structure within the daily weather generation process. Parameters can be altered in any of the components of the proposed model to enable the generation of realistic time series of climate variables that exhibit changes to both lower-order and higher-order statistics at long-term (inter-annual), mid-term (seasonal), and short-term (daily) timescales. The tool can be coupled with impact models in a bottom-up risk assessment to efficiently and exhaustively explore the potential climate changes under which a system is most vulnerable. An application of the weather generator is presented for the Connecticut River basin to demonstrate the tool's ability to generate a wide range of possible climate sequences over an extensive spatial domain.

Description: The objective of the least cost design problem of a water distribution system is to find its minimum cost with discrete diameters as decision variables and hydraulic controls as constraints. The goal of a robust least cost design is to find solutions which guarantee its feasibility independent of the data (i.e., under model uncertainty). A robust counterpart approach for linear uncertain problems is adopted in this study, which represents the uncertain stochastic problem as its deterministic equivalent. Robustness is controlled by a single parameter providing a trade-off between the probability of constraint violation and the objective cost. Two principal models are developed-uncorrelated uncertainty model with implicit design reliability, and correlated uncertainty model with explicit design reliability. The models are tested on three example applications and compared for uncertainty in consumers’ demands. The main contribution of this study is the inclusion of the ability to explicitly account for different correlations between water distribution systems demand nodes. In particular it is shown that including correlation information in the design phase has a substantial advantage in seeking more efficient robust solutions.

Description: Moving from univariate to multivariate frequency analysis, this study extends the Klemeš' critique of the widespread belief that the increasingly refined mathematical structures of probability functions increase the accuracy and credibility of the extrapolated upper tails of the fitted distribution models. In particular, we discuss key aspects of multivariate frequency analysis applied to hydrological data such as the selection of multivariate design events (i.e., appropriate subsets or scenarios of multiplets that exhibit the same joint probability to be used in design applications) and the assessment of the corresponding uncertainty. Since these problems are often overlooked or treated separately, and sometimes confused, we attempt to clarify properties, advantages, shortcomings and reliability of results of frequency analysis. We suggest a selection method of multivariate design events with prescribed joint probability based on simple Monte Carlo simulations that accounts for the uncertainty affecting the inference results and the multivariate extreme quantiles. It is also shown that the exploration of the p -level probability regions of a joint distribution returns a set of events that is a subset of the p -level scenarios resulting from an appropriate assessment of the sampling uncertainty, thus tending to overlook more extreme and potentially dangerous events with the same (uncertain) joint probability. Moreover, a quantitative assessment of the uncertainty of multivariate quantiles is provided by introducing the concept of joint confidence intervals. From an operational point of view, the simulated event sets describing the distribution of the multivariate p -level quantiles can be used to perform multivariate risk analysis under sampling uncertainty. As an example of the practical implications of this study, we analyse two case studies already presented in the literature.

Description: Angiogenin (ANG) undergoes nuclear translocation and promotes ribosomal RNA (rRNA) transcription thereby enhancing cell growth and proliferation. However, the mode of action of ANG in stimulating rRNA transcription is unclear. Here, we show that ANG enhances the formation of RNA polymerase I (Pol I) pre-initiation complex at the ribosomal DNA (rDNA) promoter. ANG binds at the upstream control element (UCE) of the promoter and enhances promoter occupancy of RNA Pol I as well as the selectivity factor SL1 components TAF I 48 and TAF I 110. We also show that ANG increases the number of actively transcribing rDNA by epigenetic activation through promoter methylation and histone modification. ANG binds to histone H3, inhibits H3K9 methylation, and activates H3K4 methylation as well as H4 acetylation at the rDNA promoter. These data suggest that one of the mechanisms by which ANG stimulates rRNA transcription is through an epigenetic activation of rDNA promoter. J. Cell. Physiol. © 2013 Wiley Periodicals, Inc.

Description: No abstract is available for this article.

Description: Pancreatic cancer (PC) has a high rate of mortality and a poorly understood mechanism of progression. Investigation of the molecular mechanism of PC and exploration of the specific markers for early diagnosis and specific targets of therapy are key points to prevent and treat PC effectively and to improve their prognosis. In our study, expression profiles experiment of para-carcinoma, carcinoma and relapse human PC was performed using Agilent human whole genomic oligonucleotide microarrays with 45 000 probes. Differentially expressed genes related with PC were screened and analysed further by Gene Ontology term analysis and Kyoto encyclopaedia of genes and genomes pathway analysis. Our results showed that there were 3853 differentially expressed genes associated with pancreatic carcinogenesis and relapse. In addition, our study found that PC was related to the Jak–STAT signalling pathway, PPAR signalling pathway and Calcium signalling pathway, indicating their potential roles in pancreatic carcinogenesis and progress. Copyright © 2013 John Wiley & Sons, Ltd.

Description: ABSTRACT Uchl1 encodes the protein gene product 9.5 antigen (PGP9.5) that is a widely used to identify migrating neural progenitors in the PNS, mature neurons of the central and peripheral nervous systems, as well as neuroendocrine cells. To facilitate analysis of developing peripheral neurons, we linked regulatory regions of Uchl1 carried within a 160kb bacterial artificial chromosome (BAC) to the dual fluorescent reporter H2BmCherry:GFP-gpi. The Uchl1 -H2BmCherry:GFP-gpi transgene exhibits robust expression and allows clear discrimination of individual cells and cellular processes in cranial ganglia, sympathetic chain, the enteric nervous system (ENS), and autonomic ganglia of the urogenital system. The transgene also labels subsets of cell in endocrine tissues where prior in situ hybridization (ISH) studies have previously identified expression of this deubiquinating enzyme. The Uchl1 -H2BmCherry:GFP-gpi transgene will be a powerful tool for static and live imaging as well as isolation of viable neural progenitors to investigate processes of autonomic neurogenesis. © 2013 Wiley Periodicals, Inc.

Description: Supraglacial channels are an important mechanism for surface water transport over the ablation zone of western Greenland. The first assessment of the spatio-temporal distribution of surface melt channels and their relationship to supraglacial lakes over the Jakobshavn Isbrae region of Western Greenland was analyzed using Landsat ETM + panchromatic images during the 2007 melt season. A total of 1188 melt channels were delineated and show an increase in the number of melt channels throughout the season, reaching a peak on August 9. Water-filled melt channels advanced to maximum elevation of 1647 m on August 9, and attained a minimum average slope of 0.009 on July 8. The ablation zone demonstrates two hydrologic modes, where crevasse and moulin terminating channels dominate at elevations 〈800 m and higher order channel networks 〉800 m. Development of higher order networks is interrupted by flow divergence due to partitioning of melt water into vertical infiltration through moulins and crevasse fields prevalent at lower elevations. Tributary and Connector networks between 800 to 1200 m in elevation are correlated with fewer lake occurrences, relatively lower surface velocities (~50 ma -1 ) and ice flow dominated by internal deformation over basal sliding. High order channels are associated with lake basins that exceed melt water storage capacity. Evolution of channel networks is coupled to changes in melt water production, runoff, and ice dynamics with implication for the englacial and subglacial environments. This article is protected by copyright. All rights reserved.

Description: This paper reviews the use of the Generalised Likelihood Uncertainty Estimation (GLUE) methodology in the 20 years since the paper by Beven and Binley (1992) in Hydrological Processes, which is now one of the most highly cited papers in hydrology. The original conception, the on-going controversy it has generated, the nature of different sources of uncertainty and the meaning of the GLUE prediction uncertainty bounds, are discussed. The hydrological, rather than statistical, arguments about the nature of model and data errors and uncertainties that are the basis for GLUE are emphasised. The application of the IHDM model to the Gwy catchment at Plynlimon presented in the original paper is revisited, using a much larger sample of models, a wider range of likelihood evaluations and new visualisation techniques. It is concluded that there are good reasons to reject this model for that data set. This is a positive result in a research environment in that it requires improved models or data to be made available. In practice, there may be ethical issues of using outputs from models for which there is evidence for model rejection in decision making. Finally some suggestions for what is needed in the next 20 years are provided. This article is protected by copyright. All rights reserved.

Description: A short-term flood inundation prediction model has been formulated based on the combination of the super-tank model, forced with downscaled rainfall from a global numerical weather prediction model, and a one-dimensional hydraulic model. Different statistical methods for downscaled rainfall have been explored, taking into account the availability of historical data. It has been found that the full implementation of a statistical downscaling model considering physically based corrections to the numerical weather prediction model output for rainfall prediction performs better compared with an altitudinal correction method. The integration of the super-tank model into the one-dimensional hydraulic model demonstrates a minimal requirement for the calibration of rainfall-runoff and flood propagation models. Updating the model with antecedent rainfall and regular forecast renewal has enhanced the model's capabilities as a result of the data assimilation processes of the runoff and numerical weather prediction models. The results show that the predicted water levels demonstrate acceptable agreement with those measured by stream gauges and comparable to those reproduced using the actual rainfall. Moreover, the predicted flood inundation depth and extent exhibit reasonably similar tendencies to those observed in the field. However, large uncertainties are observed in the prediction results in lower, flat portions of the river basin where the hydraulic conditions are not properly analysed by the one-dimensional flood propagation model. This article is protected by copyright. All rights reserved.

Description: This study aims to analyse the combined impacts of future discharges and sea levels on erosion-sedimentation potential, and its seasonal changes, in a ~43 km long coastal river reach of South-West Finland. To our knowledge, this kind of combined study has not been performed before. In addition to surveying the present erosion-sedimentation conditions, the daily erosion-sedimentation potential is simulated with a one dimensional hydrodynamic model for the 1971–2000 and 2070–2099 periods by applying four discharge scenarios. Different sea level stages are also employed in the simulations. All scenarios forecast increasing autumn and winter discharges, but diminishing summer discharges. This indicates increasing river channel erosion, particularly during winters and autumns. Although discharge changes have altogether a greater influence on erosion-sedimentation potential, the importance of sea level changes on sedimentation is noticeable in the estuary. The rising sea level scenarios increase the sedimentation potential. In total, by 2070–2099 the erosion potential may increase in most parts of the study area. This article is protected by copyright. All rights reserved.

Description: Sparse geologic dictionaries provide a novel approach for subsurface flow model representation and calibration. Learning sparse dictionaries from prior training datasets is an effective approach to describe complex geologic connectivity patterns in subsurface imaging applications. However, the computational cost of sparse learning algorithms becomes prohibitive for large models. Performing the sparse dictionary learning process on smaller image patches (segments) provides a simple approach to address this problem in image processing applications. However, in underdetermined subsurface flow model calibration inverse problems, reconstruction of a segmented image can introduce significant structural distortion and discontinuity at the boundaries of the segments. This paper proposes an alternative sparse learning approach where the sparse dictionaries are learned from low-rank representations of the large-scale training dataset in spectral domains (e.g., frequency domain). The objective is to develop a computationally efficient dictionary learning approach that emphasizes large-scale spatial connectivity patterns. This is achieved by removing the strong spatial correlations in the training data, thereby eliminating a large number of insignificant components from the sparse learning computation. In addition to improving the computational complexity, sparse learning from low-rank training datasets suppresses the small-scale details from entering the reconstruction of large-scale connectivity patterns, and providing a regularization effect in solving the resulting ill-posed inverse problems. We apply the proposed approach to travel-time tomography inversion and nonlinear subsurface flow model calibration inverse problems to demonstrate its effectiveness and practicality.

Description: Northern peatlands are a large source of atmospheric methane (CH 4 ) and both a source and sink of atmospheric carbon dioxide (CO 2 ). The rate and temporal variability in gas exchanges with peat soils is directly related to the spatial distribution of these free-phase gases within the peat column. In this paper we present results from surface and borehole ground penetrating radar (GPR) surveys – constrained with direct soil and gas sampling – that compare the spatial distribution of gas accumulations in two raised bogs: one in Wales (UK), the other in Maine (USA). Although the two peatlands have similar average thickness, physical properties of the peat matrix differ, particularly in terms of peat type and degree of humification. We hypothesize that these variations in physical properties are responsible for the differences in gas distribution between the two peatlands characterized by: 1) gas content up to 10.8 % associated with woody peat and presence of wood layers in Caribou Bog (Maine), and 2) a more homogenous distribution with gas content up to 5.7 % at the surface (i.e. 〈 0.5 m deep) in Cors Fochno (Wales). Our results highlight the variability in biogenic gas accumulation and distribution across peatlands and suggest that the nature of the peat matrix has a key role in defining how biogenic gas accumulates within, and is released to the atmosphere from, peat soils. This article is protected by copyright. All rights reserved.

Description: The confounding effects of step change invalidate the stationarity assumption of commonly used trend analysis methods such as the Mann-Kendall test technique, so previous studies have failed to explain inconsistencies between detected trends and observed large precipitation anomalies. The objectives of this study were to: 1) formulate a trend analysis approach that considers nonstationarity due to step changes; 2) use this approach to detect trends and extreme occurrences of precipitation in a mid-latitude Eurasian steppe watershed in north China; and 3) examine how runoff responds to precipitation trends in the study watershed. Our results indicate that annual precipitation underwent a marginal step jump around 1995. The significant annual downward trend after 1994 was primarily due to a decrease in summer rainfall; other seasons exhibited no significant precipitation trends. At a monthly scale, July rainfall after 1994 exhibited a significant downward trend, whereas precipitation in other months had no trend. The percentage of wet days also underwent a step jump around 1994 following a significant decreasing trend, although the precipitation intensity exhibited neither a step change nor any significant trend. However, both low- and high-frequency precipitation events in the study watershed occurred more often after than before 1994, probably as either a result or an indicator of climate change. In response to these precipitation changes, the study watershed had distinctly different precipitation-runoff relationships for observed annual precipitations of less than 300 mm, between 300 and 400 mm, and greater than 400 mm. This article is protected by copyright. All rights reserved.

Description: Characterizing the specific inherent optical properties (SIOPs) of water constituents is fundamental to remote sensing applications. Therefore, this paper presents the absorption properties of phytoplankton, gelbstoff and tripton for three small, optically-diverse South African inland waters. The three reservoirs,  Hartbeespoort, Loskop and Theewaterskloof, are challenging for remote sensing, due to differences in phytoplankton assemblage and the considerable range of constituent concentrations. Relationships between the absorption properties and biogeophysical parameters, chlorophyll-a (chl-a), TChl (chl-a plus  phaeopigments),  seston,  minerals  and  tripton, are established. The value determined for the mass-specific tripton absorption coefficient at 442 nm, a∗ (442), ranges from 0.024 to 0.263 m2·g−1. The value of the TChl-specific phytoplankton absorption coefficient (a∗ ) was strongly influenced by phytoplankton species, size, accessory pigmentation and biomass. a∗ (440) ranged from 0.056 to 0.018 m2·mg−1 in oligotrophic to hypertrophic waters. The positive relationship between cell size and trophic state observed in open ocean waters was violated by significant small cyanobacterial populations. The phycocyanin-specific phytoplankton  absorption  at  620  nm,  a∗ (620), was determined as 0.007 m2·g−1 in a M. aeruginosa bloom. Chl-a was a better indicator of phytoplankton biomass than phycocyanin (PC) in surface scums, due to reduced accessory pigment production. Absorption budgets demonstrate that monospecific blooms of M. aeruginosa and C. hirundinella may be treated as “cultures”, removing some complexities for remote sensing applications.   These results contribute toward a better understanding of IOPs and remote sensing applications in hypertrophic inland waters. However, the majority of the water is optically complex, requiring the usage of all the SIOPs derived here for remote sensing applications. The SIOPs may be used for developing remote sensing algorithms for the detection of biogeophysical  parameters,  including chl-a, suspended matter, tripton and gelbstoff, and in advanced remote sensing studies for phytoplankton type detection.

Description: Landcover change alters not only the surface landscape but also regional carbon and water cycling. The objective of this study was to assess the potential impacts of landcover change across the Kansas River Basin (KRB) by comparing local microclimatic impacts and regional scale climate influences. This was done using a 25-year time series of Normalized Difference Vegetation Index (NDVI) and precipitation (PPT) data analyzed using multi-resolution information theory metrics. Results showed both entropy of PPT and NDVI varied along a pronounced PPT gradient. The scalewise relative entropy of NDVI was the most informative at the annual scale, while for PPT the scalewise relative entropy varied temporally and by landcover type. The relative entropy of NDVI and PPT as a function of landcover showed the most information at the 512-day scale for all landcover types, implying different landcover types had the same response across the entire KRB. This implies that land use decisions may dramatically alter the local time scales of responses to global climate change. Additionally, altering land cover (e.g., for biofuel production) may impact ecosystem functioning at local to regional scales and these impacts must be considered for accurately assessing future implications of climate change.

Description: Hydrological models are useful tools to analyze present and future conditions of water quantity and quality. The integrated modeling of water and nutrients needs an adequate representation of the different discharge components. In common with many lowlands, groundwater contribution to the discharge in the North German lowlands is a key factor for a reasonable representation of the water balance especially in low flow periods. Several studies revealed that the widely used SWAT model performs poorly for low flow periods. This paper deals with the extension of the groundwater module of the SWAT model to enhance low flow representation. The current two-storage concept of SWAT was further developed to a three-storage-concept. This was realized due to modification of the groundwater module by splitting the active roundwater storage into a fast and a slow contributing aquifer. The results of this study show that the groundwater module with three storages leads to good prediction of the overall discharge especially for the recession limbs and the low flow periods. The improved performance is reflected in the signature measures for the mid segment (PBIAS: −2:4% vs. −15:9%) and the low segment (PBIAS: 14:8% vs. 46:8%) of the flow duration curve. The three-storage groundwater module is more process oriented than the original version due to the introduction of a fast and a slow groundwater flow component. The three-storage version includes a modular approach, since groundwater storages can be activated or deactivated independently for subbasin and HRU level. This article is protected by copyright. All rights reserved.

Description: The Receiver Operating Characteristic (ROC) is widely used for assessing the performance of classification algorithms. In GIScience, ROC has been applied to assess models aimed at predicting events, such as land use/cover change (LUCC), species distribution and disease risk. However, GIS software packages offer few statistical tests and guidance tools for ROC analysis and interpretation. This paper presents a suite of GIS tools designed to facilitate ROC curve analysis for GIS users by applying proper statistical tests and analysis procedures. The tools are freely available as models and submodels of Dinamica EGO freeware. The tools give the ROC curve, the area under the curve (AUC), partial AUC, lower and upper AUCs, the confidence interval of AUC, the density of event in probability bins and tests to evaluate the difference between the AUCs of two models. We present first the procedures and statistical tests implemented in Dinamica EGO, then the application of the tools to assess LUCC and species distribution models. Finally, we interpret and discuss the ROC-related statistics resulting from various case studies.

Description: The current study reports on contact interference of a high-level bacitracin- resistant pheromone-responsive plasmid of Enterococcus faecalis strain 543 of poultry origin during conjugative transfer of bcr antimicrobial resistance genes using a polyclonal antiserum aggregation substance44–560 (AS). After induction with pheromones produced by the recipient strain E. faecalis JH2-2, clumping of the donor E. faecalis strain 543 was observed as well as high transfer frequencies of bcr in short time broth mating. Filter mating assays from donor strain E. faecalis 543 to the recipient strain E. faecalis JH2-2 revealed conjugative transfer of asa1 (AS), bcrRAB and traB (negative regulator pheromone response) genes. The presence of these genes in transconjugants was confirmed by antimicrobial susceptibility testing, PCR, Southern hybridization and sequencing. A significant reduction in formation of aggregates was observed when the polyclonal anti-AS44–560 was added in the pheromone-responsive conjugation experiments as compared to the induced state. Moreover, interference of anti-AS44–560 antibodies in pheromone-responsive conjugation was demonstrated by a reduction in horizontal transfer of asa1 and bcr genes between E. faecalis strain 543 and E. faecalis JH2-2. Reducing the pheromone-responsive conjugation of E. faecalis is of interest because of its clinical importance in the horizontal transfer of antimicrobial resistance.

Description: The Nightfire algorithm detects and characterizes sub-pixel hot sources using multispectral data collected globally, each night, by the Suomi National Polar Partnership (NPP) Visible Infrared Imaging Radiometer Suite (VIIRS). The spectral bands utilized span visible, near-infrared (NIR), short-wave infrared (SWIR), and mid-wave infrared (MWIR). The primary detection band is in the SWIR, centered at 1.6 μm. Without solar input, the SWIR spectral band records sensor noise, punctuated by high radiant emissions associated with gas flares, biomass burning, volcanoes, and industrial sites such as steel mills. Planck curve fitting of the hot source radiances yields temperature (K) and emission scaling factor (ESF). Additional calculations are done to estimate source size (m2), radiant heat intensity (W/m2), and radiant heat (MW). Use of the sensor noise limited M7, M8, and M10 spectral bands at night reduce scene background effects, which are widely reported for fire algorithms based on MWIR and long-wave infrared. High atmospheric transmissivity in the M10 spectral band reduces atmospheric effects on temperature and radiant heat retrievals. Nightfire retrieved temperature estimates for sub-pixel hot sources ranging from 600 to 6,000 K. An intercomparison study of biomass burning in Sumatra from June 2013 found Nightfire radiant heat (MW) to be highly correlated to Moderate Resolution Imaging Spectrometer (MODIS) Fire Radiative Power (MW).

Description: The cytoplasmic signaling protein tumor necrosis factor (TNF) receptor-associated factor 5 (TRAF5), which was identified as a signal transducer for members of the TNF receptor super-family, has been implicated in several biological functions in T/B lymphocytes and the innate immune response against viral infection. However, the role of TRAF5 in cardiac hypertrophy has not been reported. In the present study, we investigated the effect of TRAF5 on the development of pathological cardiac hypertrophy induced by transthoracic aorta constriction (TAC) and further explored the underlying molecular mechanisms. Cardiac hypertrophy and function were evaluated with echocardiography, hemodynamic measurements, pathological and molecular analyses. For the first time, we found that TRAF5 deficiency substantially aggravated cardiac hypertrophy, cardiac dysfunction and fibrosis in response to pressure overload after 4 weeks of TAC compared to wild-type (WT) mice. Moreover, the mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK)-extracellular signal-regulated kinases 1/2 (ERK1/2) signaling pathway was more activated in TRAF5-deficient mice than WT mice. In conclusion, our results suggest that as an intrinsic cardioprotective factor, TRAF5 plays a crucial role in the development of cardiac hypertrophy through the negative regulation of the MEK-ERK1/2 pathway. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: The SON protein is a ubiquitously expressed DNA- and RNA-binding protein primarily localized to nuclear speckles. Although several early studies implicated SON in DNA-binding, tumorigenesis and apoptosis, functional significance of this protein had not been recognized until recent studies discovered SON as a novel RNA splicing co-factor. During constitutive RNA splicing, SON ensures efficient intron removal from the transcripts containing suboptimal splice sites. Importantly, SON-mediated splicing is required for proper processing of selective transcripts related to cell cycle, microtubules/centrosomes maintenance, and genome stability. Moreover, SON regulates alternative splicing of RNAs from the genes involved in apoptosis and epigenetic modification. In addition to the role in RNA splicing, SON has an ability to suppress transcriptional activation at certain promoter/enhancer DNA sequences. Considering the multiple SON target genes which are directly involved in cell proliferation, genome stability and chromatin modifications, SON is an emerging player in gene regulation during cancer development and progression. Here, we summarize available information from several early studies on SON, and highlight recent discoveries describing molecular mechanisms of SON-mediated gene regulation. We propose that our future effort on better understanding of diverse SON functions would reveal novel targets for cancer therapy. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.

Description: The ecological condition and biodiversity values of floodplain wetlands are highly dependent on the hydrological connectivity of wetlands to adjacent rivers. This paper describes a method for quantifying connectivity between floodplain wetlands and the main rivers in a wet tropical catchment of northern Australia. We used a 1-D hydrodynamic model to simulate time-varying water depths across the stream network (i.e. rivers, streams and man-made drains). The timing and duration of connectivity of seven wetlands (4 natural and 3 artificial) with the two main rivers in the catchment were then calculated for different hydrological conditions. Location and areal extent of the wetlands and the stream network were identified using high resolution laser altimetry (LiDAR) and these data formed key inputs to the hydrodynamic model. The model was calibrated using measured water depths and discharges across the floodplain. An algorithm was developed to identify contiguous water bodies at daily time steps and this gave the temporal history of connection and disconnection between wetlands and the rivers. Simulation results show that connectivity of individual wetlands to both rivers varies from 26 to 365 days during an average hydrological condition. Location, especially proximity to a main river, and wetland type (natural stream or artificial drain) were identified as key factors influencing these levels of connectivity. Some natural wetlands maintain connection with the river for most or all of the year, whereas the connectivity of some artificial wetlands varies from 26 to 36 days according to their patterns of network connection to adjacent rivers – a result that has important implications for the accessibility of these types of wetland to aquatic biota. Using readily available river gauge data, we also show how connectivity modelling can be used to identify periods when connectivity has fallen below critical thresholds for fish movement. These connectivity patterns within the floodplain network are central to the setting of river flows that will meet environmental requirements for biota that use floodplain wetlands during their life history. This article is protected by copyright. All rights reserved.

Description: Pumping tests are one of the most commonly used in-situ testing techniques for assessing aquifer hydraulic properties. Numerous researches have been conducted to predict the effects of aquifer heterogeneity on the groundwater levels during pumping tests. The objectives of the present work were 1) to predict drawdown conditions and estimate aquifer properties during pumping tests undertaken in radially symmetric heterogeneous aquifers and 2) to identify a method for assessing the transmissivity field along the radial coordinate in radially symmetric and fully heterogeneous transmissivity fields. The first objective was achieved by expanding an existing analytical drawdown formulation which was valid for a radially symmetric confined aquifer with two concentric zones around the pumping well to an N concentric zone confined aquifer having a constant transmissivity value within each zone. The formulation was evaluated for aquifers with three and four concentric zones to assess the effects of the transmissivity field on the drawdown conditions. The specific conditions under which aquifer properties could be identified using traditional methods of analysis were also evaluated. The second objective was achieved by implementing the Inverse Solution Algorithm (ISA) which was developed for petroleum reservoirs to groundwater aquifer settings. The results showed that the drawdown values are influenced by a volumetric integral of a weighting function and the transmissivity field within the cone of depression. The weighting function migrates in tandem with the expanding cone of depression. The ability of the ISA to predict radially symmetric and log-normally distributed transmissivity fields was assessed against analytical and numerical benchmarks. The results of this investigation indicated that the ISA method is a viable technique for evaluating the radial transmissivity variations of heterogeneous aquifer settings. This article is protected by copyright. All rights reserved.

Description: Antibiotic resistance can be reduced by using antibiotics prudently based on guidelines of antimicrobial stewardship programs (ASPs) and various data such as pharmacokinetic (PK) and pharmacodynamic (PD) properties of antibiotics, diagnostic testing, antimicrobial susceptibility testing (AST), clinical response, and effects on the microbiota, as well as by new antibiotic developments. The controlled use of antibiotics in food animals is another cornerstone among efforts to reduce antibiotic resistance. All major resistance-control strategies recommend education for patients, children (e.g., through schools and day care), the public, and relevant healthcare professionals (e.g., primary-care physicians, pharmacists, and medical students) regarding unique features of bacterial infections and antibiotics, prudent antibiotic prescribing as a positive construct, and personal hygiene (e.g., handwashing). The problem of antibiotic resistance can be minimized only by concerted efforts of all members of society for ensuring the continued efficiency of antibiotics.

Description: The increasing availability of satellite imagery acquired by existing and new sensors allows a wide variety of new applications that depend on the use of diverse spectral and spatial resolution data sets. One of the pre-conditions for the use of hybrid image data sets is a consistent geo-correction capacity. We demonstrate how a novel fast template matching approach implemented on a graphics processing unit (GPU) allows us to accurately and rapidly geo-correct imagery in an automated way. The key difference with existing geo-correction approaches, which do not use a GPU, is the possibility to match large source image segments (8,192 by 8,192 pixels) with relatively large templates (512 by 512 pixels) significantly faster. Our approach is sufficiently robust to allow for the use of various reference data sources. The need for accelerated processing is relevant in our application context, which relates to mapping activities in the European Copernicus emergency management service. Our new method is demonstrated over an area northwest of Valencia (Spain) for a large forest fire event in July 2012. We use the Disaster Monitoring Constellation’s (DMC) DEIMOS-1 and RapidEye imagery for the delineation of burnt scar extent. Automated geo-correction of each full resolution image set takes approximately one minute. The reference templates are taken from the TerraColor data set and the Spanish national ortho-imagery database, through the use of dedicated web map services. Geo-correction results are compared to the vector sets derived in the Copernicus emergency service activation request.

Description: The mixed grassland in Canada is characterized by low to medium green vegetation cover, with a large amount of canopy background, such as non-photosynthetic vegetation residuals (litter), bare soil, and ground level biological crust. It is a challenge to extract the canopy information from satellite images because of the influence of canopy background. Therefore, this study aims to extract a soil line, a representation of bare soil with litter and soil crust in the surface, from Landsat images to reduce the background effect. Field work was conducted in the West Block of Grasslands National Park (GNP) in Canada, which represents the northern mixed grassland from late June to early July 2005. Six TM images with either no or only a small amount of cloud content were collected in 2005. In this study, soil lines were extracted directly from images by quantile regression and the (R, NIRmin) method. The results show that, (1) both cloud and cloud shadow have obvious influence on simulating soil line automatically from images; (2) green up and late senescence seasons are relatively better for soil line simulation; (3) the (R, NIRmin) method is better for soil line simulation than quantile regression to extract green biomass or green cover information.

Description: In the present study, we examined the effects of methanol extracts of Picrasma quassioides (MEPQ) on apoptosis in human cervical cancer cells. The results showed that MEPQ decreased the viability and induced caspase-dependent apoptosis in HEp-2 cells. MEPQ decreased specificity protein 1 (Sp1) in HEp-2 cells, whereas Sp1 mRNA was not changed. We found that MEPQ reduced Sp1 protein through proteasome-dependent protein degradation, but not the inhibition of protein synthesis. Also, MEPQ increased the expressions of Bad and truncated Bid (t-Bid) but did not alter other Bcl-2 family members. The knock-down of Sp1 by both Sp1 interfering RNA and Mithramycin A, Sp1 specific inhibitor clearly increased Bad and t-Bid expression to decrease cell viability and induce apoptosis. In addition, MEPQ inhibited cell viability and induced apoptotic cell death through the modulation of Sp1 in KB cells. These results suggest that MEPQ may be a potential anticancer agent for human cervical cancer. Copyright © 2013 John Wiley & Sons, Ltd.

Description: During October, November and December 2011 (when highest sales of Agri-Hub fresh produce are observed), irrigation water, compost, lettuce and spinach sampled from four different farmer cooperatives supplying the local Agri-Hub in uMbumbulu (KwaZulu-Natal, South Africa) were analyzed monthly for the presence of total and fecal coliforms and Escherichia coli using the most probable number (MPN) technique. The pH values for all irrigation water samples analyzed were within the acceptable range of 6.5–8.5 for agricultural use. Fecal coliform levels were 〈1,000 MPN per 100 mL irrigation water and 〈1,000 MPN per g of compost. The vegetables produced by Agri-Hub small-scale farmers met the requirements for total coliforms of 〈200/g set by the South African Department of Health at the time of sampling. E. coli MPN values for irrigation water and vegetables were below the limit of detection. In addition, the farming practices of 73 farmers were assessed via a survey. The results revealed that more than 40% of farmers used microbiologically safe tap water for irrigation and that trained farmers have a significantly better understanding of the importance of production hygiene than untrained farmers. These results reiterate the importance of interventions that build capacity in the area of food safety and hygiene of small-scale farmers for market access of formal value chains.

Description: The pharmaceutical industry’s profitability depends on identifying and successfully developing new drug candidates while trying to contain the increasing costs of drug development. It is actively searching for new sources of innovative compounds and for mechanisms to reduce the enormous costs of developing new drug candidates. There is an opportunity for academia to further develop as a source of drug discovery. The rising levels of industry outsourcing also provide prospects for organisations that can reduce the costs of drug development. We explored the potential returns to New Zealand (NZ) from its drug discovery expertise by assuming a drug development candidate is out-licensed without clinical data and has anticipated peak global sales of $350 million. We also estimated the revenue from NZ’s clinical research industry based on a standard per participant payment to study sites and the number of industry-sponsored clinical trials approved each year. Our analyses found that NZ’s clinical research industry has generated increasing foreign revenue and appropriate policy support could ensure that this continues to grow. In addition the probability-based revenue from the out-licensing of a drug development candidate could be important for NZ if provided with appropriate policy and financial support.

Description: General mechanism for exaggerated sexuallyselected traits. Many animals wield sexually-selected exaggerated traits. ‘Classic’ examples include the peacock's train, and the antlers observed in male deer, as well as fiddler crabs with enlarged claws, and the enlarged head-horns of rhinoceros beetles ( Trypoxylus dichotomus , cover). These traits are used for mate choice, or to deter rival males, because they act as unusually reliable signals of the condition of individual males: only the best-quality animals produce full-sized signal structures. But what keeps their expression honest? How can signal traits evolve that are resistant to invasion by cheaters who fake attractive signals? The answer may lie in the ancient insulin-like signalling pathway, which is found in all animals and directly links individual condition to growth in a dose dependent manner. Warren et al. discuss recent evidence suggesting that exaggerated sexually-selected signal traits arise when specific structures become extra-sensitive to physiological signals like insulins or insulin-like growth factors (pages 889–899 ).

Description: A new hybrid wavelet–bootstrap–neural network (WBNN) model is proposed in this study for short term (1, 3 and 5 day; 1 and 2 week; and 1 and 2 month) urban water demand forecasting. The new method was tested using data from the city of Montreal in Canada. The performance of the WBNN method was compared with the autoregressive integrated moving average (ARIMA) and autoregressive integrated moving average model with exogenous input variables (ARIMAX), traditional NNs, wavelet analysis based NNs (WNN), bootstrap based NNs (BNN), and a simple naïve persistence index model. The WBNN model was developed as an ensemble of several NNs built using bootstrap resamples of wavelet sub-time series instead of raw datasets. The results demonstrated that the hybrid WBNN and WNN models produced significantly more accurate forecasting results than the traditional NN, BNN, ARIMA and ARIMAX models. It was also found that the WBNN model reduces the uncertainty associated with the forecasts, and the performance of WBNN forecasted confidence bands were found to be more accurate and reliable than BNN forecasted confidence bands. It was found in this study that maximum temperature and total precipitation improved the accuracy of water demand forecasts using wavelet analysis. The performance of WBNN models was also compared for different numbers of bootstrap resamples (i.e., 25, 50, 100, 200, and 500) and it was found that WBNN models produced optimum results with different numbers of bootstrap resamples for different lead time forecasts with considerable variability.

Description: mRNA synthesis in all organisms is performed by RNA polymerases, which work as nanomachines on DNA templates. The rate at which their product is made is an important parameter in gene expression. Transcription rate encompasses two related, yet different, concepts: the nascent transcription rate, which measures the in situ mRNA production by RNA polymerase, and the rate of synthesis of mature mRNA, which measures the contribution of transcription to the mRNA concentration. Both parameters are useful for molecular biologists, but they are not interchangeable and they are expressed in different units. It is important to distinguish when and where each one should be used. We propose that for functional genomics the use of nascent transcription rates should be restricted to the evaluation of the transcriptional process itself, whereas mature mRNA synthesis rates should be employed to address the transcriptional input to mRNA concentration balance leading to variation of gene expression. Transcription rate encompasses two related, yet different, concepts: the nascent transcription rate, which measures the in situ mRNA production by RNA polymerase, and the rate of synthesis of mature mRNA, which measures the contribution of transcription to the mRNA concentration. It is important to distinguish when to use each one.

Description: CALHM1 was recently demonstrated to be a voltage-gated ATP-permeable ion channel and to serve as a bona fide conduit for ATP release from sweet-, umami-, and bitter-sensing type II taste cells. Calhm1 is expressed in taste buds exclusively in type II cells and its product has structural and functional similarities with connexins and pannexins, two families of channel protein candidates for ATP release by type II cells. Calhm1 knockout in mice leads to loss of perception of sweet, umami, and bitter compounds and to impaired gustatory nerve responses to these tastants. These new studies validate the concept of ATP as the primary neurotransmitter from type II cells to gustatory neurons. Furthermore, they identify voltage-gated ATP release through CALHM1 as an essential molecular mechanism of ATP release in taste buds. We discuss these new findings, as well as unresolved issues in peripheral taste signaling that we hope will stimulate future research. Sweetness, umami, and bitterness are transmitted to the nervous system via ion channel-mediated ATP release from taste cells. A recent study demonstrated that CALHM1 is essential for taste cell ATP release and perception of sweetness, umami, and bitterness. We discuss the new findings and unresolved issues in peripheral taste signaling.

Description: Neural tube defects (NTDs) are common complex congenital malformations resulting from failure of the neural tube closure during embryogenesis. It is established that folic acid supplementation decreases the prevalence of NTDs, which has led to national public health policies regarding folic acid. To date, animal studies have not provided sufficient information to establish the metabolic and/or genomic mechanism(s) underlying human folic acid responsiveness in NTDs. However, several lines of evidence suggest that not only folates but also choline, B12 and methylation metabolisms are involved in NTDs. Decreased B12 vitamin and increased total choline or homocysteine in maternal blood have been shown to be associated with increased NTDs risk. Several polymorphisms of genes involved in these pathways have also been implicated in risk of development of NTDs. This raises the question whether supplementation with B12 vitamin, betaine or other methylation donors in addition to folic acid periconceptional supplementation will further reduce NTD risk. The objective of this article is to review the role of methylation metabolism in the onset of neural tube defects.

Description: Zimbabwe’s economy declined between 2000 and 2009. This study detects the economic decline in different regions of Zimbabwe using nighttime light imagery from the Defense Meteorological Satellite Program’s Operational Linescan System (DMSP-OLS). We found a good correlation (coefficient = 0.7361) between Zimbabwe’s total nighttime light (TNL) and Gross Domestic Product (GDP) for the period 1992 to 2009. Therefore, TNL was used as an indicator of regional economic conditions in Zimbabwe. Nighttime light imagery from 2000 and 2008 was compared at both national and regional scales for four types of regions. At the national scale, we found that nighttime light in more than half of the lit area decreased between 2000 and 2008. Moreover, within the four region types (inland mining towns, inland agricultural towns, border towns and cities) we determined that the mining and agricultural sectors experienced the most severe economic decline. Some of these findings were validated by economic survey data, proving that the nighttime light data is a potential data source for detecting the economic decline in Zimbabwe.

Description: High quality sedimentary measurements are required for studying fluvial geomorphology and hydrological processes e.g., flood and river dynamics. Mobile laser scanning (MLS) currently provides the opportunity to achieve high precision measurements of coarse fluvial sediments in a large survey area. Our study aims to investigate the capability of single-track MLS data for individual particle-based sediment modeling. Individual particles are firstly detected and delineated from a digital surface model (DSM) that is generated from the MLS data. 3D MLS points of each detected individual particle are then extracted from the point cloud. The grain size and the sphericity as well as the orientation of each individual particle are estimated based on the extracted MLS points. According to the evaluations conduced in the paper, it is possible to detect and to model sediment particles above 63 mm from a single-track MLS point cloud with a high reliability. The paper further discusses the strength and the challenges of individual particle-based approach for sedimentary measurement.