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  • Articles  (12)
  • Chemical Engineering
  • Electronic structure and strongly correlated systems
  • Saccharomyces cerevisiae
  • Springer  (12)
  • 1975-1979  (12)
  • 1977  (12)
Collection
  • Books
  • Articles  (12)
Keywords
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  • 1975-1979  (12)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 115 (1977), S. 55-60 
    ISSN: 1432-072X
    Keywords: Malate dehydrogenase ; Inactivation ; Glucose metabolism ; Glyceraldehyde-3-phosphate ; Saccharomyces cerevisiae ; Glyoxylate cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cytoplasmic malate dehydrogenase in the yeast Saccharomyces cerevisiae is known to be inactivated by a glucose dependent process. In this paper it is shown that in vivo effectors of the glucose metabolism (arsenate, iodoacetate, acetaldehyde) inhibit the inactivation or change the inactivation kinetics. In vitro it was possible to inactivate the malate dehydrogenase by addition of the glucose metabolite glyceraldehyde 3-phosphate. The physiological relevance of this modification and the effect of malate dehydrogenase inactivation on the glyoxylate cycle in yeast is discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 114 (1977), S. 77-81 
    ISSN: 1432-072X
    Keywords: Mannoproteins ; Dolichyl monophosphate mannose ; Subcellular site of glycosylation ; Secretion ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Membranes of Saccharomyces cerevisiae were separated on urografin gradients. The specific activity of the light membranes (endoplasmic reticulum), the Golgi-like vesicles and the plasma membrane in transferring mannosyl residues from GDP-mannose to mannoproteins and to dolichyl monophosphate has been determined. The first mannose of the O-glycosidically linked manno-oligosaccharides is incorporated with the highest specific activity by the endoplasmic reticulum. The incorporation of the second to fourth mannosyl groups is catalysed with increasing activity also by the Golgi-like vesicles and the plasma membrane. The incorporation of mannosyl groups into weak alkali-stable positions (N-glycosidically linked chains) is carried out with almost the same specific activity by all three membrane fractions, however, dolicholdependent and-independent steps could not be distinguished as yet. The results are discussed in terms of a sequential addition of sugar residues along the route of export of the mannoproteins. The dolichol-dependent steps seem to occur on the endoplasmic reticulum and thus very carly in the event.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 113 (1977), S. 293-302 
    ISSN: 1432-072X
    Keywords: Aminopterin ; Saccharomyces cerevisiae ; Polyploid ; Oxidative-fermentative yeast ; Ultrastructure ; Bioassay ; Synchrony
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a related brewing study detailed characteristics of fermentations displaying effective yeastaminopterin interaction were presented. Fermentative yeast types (certain Saccharomyces species and Selenotila intestinalis) proved effective aminopterin reactors whereas oxidative yeasts (certain Candida, Cryptococcus, Pichia, Rhodotorula, Saccharomyces, and Trigonopsis species) proved ineffective reactors. In general effective reactors were polyploids characterized by the lack of film or pellicle formation and ineffective reactors the opposite. In stationary fermentations the Fleischmann 139 strain of S. cerevisiae proved a fair reactor. When aerated it proved an ineffective reactor and aminopterin or products there-of stimulated growth. Conversely aeration enhanced aminopterin activity of effective reactor yeasts. The positive effect of biotin on aminopterin activity and the negative effect of yeast extract, L-asparagine, adenine and thymine is shown and compared and contrasted with earlier reported studies. These findings supported by outside data suggest that oxidative yeasts (and bacteria) can readily elicit enzymes capable of inactivating aminopterin whereas fermentative types are lacking in this capability. Finally that past yeast-aminopterin studies were conducted with oxidative yeast types. Advantages of effective aminopterin reactor yeasts to be published elsewhere include improved ultrastructure using KMnO4−OsO4 fixation, a yeast bioassay procedure for detecting aminopterin in plasma and urine, and cell synchronization.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-072X
    Keywords: Environment ; Kluyveromyces fragilis ; Lipids ; Saccharomyces cerevisiae ; Sterol esters ; Triacylglycerols ; Vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Saccharomyces cerevisiae, grown aerobically or anaerobically under conditions which induce a requirement for a sterol and an unsaturated fatty acid, synthesized approximately the same amounts of neutral lipid and intracellular low-density vesicles, although the neutral lipids in aerobically-grown cells contained more esterified sterol and less triacylglycerol than those in anaerobically-grown cells. Kluyveromyces fragilis synthesized much less neutral lipid and a smaller quantity of low-density vesicles than S. cerevisiae whether grown at 30°C (generation time 1.1 h) or 20°C (generation time 2.1 h). Both yeasts synthesized highly saturated triacylglycerols, relatively unsaturated phospholipids, and esterified sterols with an intermediate degree of unsaturation irrespective of the conditions under which they were grown. Free sterols in the yeasts were rich in ergosterol and 22(24)-dehydroergosterol, while the esterified sterol fractions were richer in zymosterol.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 113 (1977), S. 159-161 
    ISSN: 1432-072X
    Keywords: Baker's yeast ; Spheroplasts ; Gluconeogenesis ; Location ; Density gradient centrifugation ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The subcellular location of hexose diphosphatase, phosphoenolpyruvate carboxykinase and pyruvate carboxylase in baker's yeast (Saccharomyces cerevisiae) was investigated by density gradient centrifugation of spheroplast lysates obtained by osmotic shock treatment of spheroplasts and centrifugation for 10000 g x min. On the evidence obtained from zonal separations these three enzymes of gluconeogenesis are most probably located in the soluble cytosol.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 114 (1977), S. 287-288 
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Mating reaction ; Sexual agglutination ; Temperature dependency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Temperature dependency of sexual agglutination in Saccharomyces cerevisiae was found. Of 31 strains tested, which showed normal agglutination when cultured at 25°C, 29 strains lost their sexual agglutinability when they were grown at 37°C.
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  • 7
    ISSN: 1432-072X
    Keywords: Yeast flocculation ; Chemical modification of cell surface components ; Floc-forming ability ; Brewer's yeast ; Saccharomyces cerevisiae ; Deflocculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effects of treatments with proteolytic enzymes and protein-modifying reagents on flocculation of brewer's yeast IFO 2018 were investigated. The floc-forming ability of the yeast cells was irreversibly eliminated by treatment with papain, trypsin, chymotrypsin or pepsin, indicating that certain proteins on the cell surface participate in the yeast flocculation. Chemical modification with reagents, known to act on disulfide bridges, carboxyl and/or phosphate groups, phenolic groups, amino groups, and imidazole groups, also destroyed the ability to flocculate, although in some cases a high concentration (8 M) of urea was necessary in addition to protein-modifying reagents. Thus, it is suggested strongly that these functional groups of amino acid residues of the proteins are essential for the floc-forming ability of brewer's yeast cells.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 113 (1977), S. 303-307 
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Specific growth rate ; Growth control ; Glucose transfer ; Glucose-6-phosphate ; Maintenance requirements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The specific growth rate (μ) of a respiration-deficient mutant of Saccharomyces cerevisiae growing under defined experimental conditions in batch culture (mineral medium plus glucose and vitamins at 25°C) varied from experiment to experiment over a wide range (0.10–0.24 h-1) and showed a normal distribution. Neither the age of the culture, the history of the inoculum, nor experimental error accounted wholy for the variability of μ. The variation was positively correlated with the specific rate of glucose transfer and negatively with the specific rate of production of non-fermentative CO2. The yield decreased with μ implying higher maintenance requirements in batch culture (4.7 mmoles g-1 h-1) than in continuous culture (0.8 mmoles g-1 h-1). It was concluded that the strain is capable of establishing any one of several steady states of growth under the same experimental conditions, each steady state displaying some buildin inertia with respect to change. The variations of the specific rates of glucose transfer and non-fermentative CO2 production, and of the yield appeared to be consequences rather than causes of the variation of μ. The ultimate causes of the variation of μ remained unidentified.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 114 (1977), S. 91-92 
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Cell wall ; Glucan ; Mannan ; Synchronous culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mode of increase in cell wall polysaccharides of yeast (glucan and mannan) during cell cycle was analyzed using cell wall samples obtained from a synchronous culture. The increase in mannan and total glucan proceeded almost linearly throughout the cell cycle except for a short period of their leveling off at the time of cell division. However, the constituents of glucan behaved characteristically: Alkalisoluble glucan and insoluble glucan increased mainly in the former and the latter half of the cell cycle, respectively.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 115 (1977), S. 307-316 
    ISSN: 1432-072X
    Keywords: Anthranilate synthase, feedback inhibition of ; Saccharomyces cerevisiae ; Tryptophan analogues, mode of action of ; Tryptophan biosynthetic enzymes ; Tryptophan pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In an analysis of the effects of various tryptophan and indole analogues in Saccharomyces cerevisiae we determined the mechanisms by which they cause growth inhibition: 4-Methyltryptophan causes a reduction in protein synthesis and a derepression of the tryptophan enzymes despite of the presence of high internal levels of tryptophan. This inhibition can only be observed in a mutant with increased permeability to the analogue. These results are consistent with but do not prove an interference of this analogue with the charging of tryptophan onto tRNA. 5-Methyltryptophan causes false feedback inhibition of anthranilate synthase, the first enzyme of the tryptophan pathway. This inhibits the further synthesis of tryptophan and results in results in tryptophan limitation, growth inhibition and derepression of the enzymes. Derepression eventually allows wild type cells to partially overcome the inhibitory effect of the analogue. 5-Fluoroindole is converted endogenously to 5-fluorotryptophan by tryptophan synthase. Both endogenous and externally supplied 5-fluorotryptophan are incorporated into protein. This leads to intoxication of the cells due to the accumulation of faulty proteins. 5-Fluorotryptophan also causes feedback inhibition of anthranilate synthase and reduces the synthesis of tryptophan which would otherwise compete with the analogues in the charging reaction. Indole acrylic acid inhibits the conversion of indole to tryptophan by tryptophan synthase. This results in a depletion of the tryptophan pool which, in turn, causes growth inhibition and derepression of the tryptophan enzymes.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 115 (1977), S. 1-7 
    ISSN: 1432-072X
    Keywords: Cell walls ; Chitin ; Colloidal gold ; Concanavalin A ; Cytochemistry ; Mannan ; Wheat germ agglutinin ; Yeast ; Saccharomyces cerevisiae ; Candida utilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mannan was located on thin sections of Saccharomyces cerevisiae and Candida utilis with the homologous anti-mannan antibodies or with Concanavalin A, both labelled with gold granules. Fully synthesized mannan was found in the cell walls, on the plasmalemma and within the cytoplasm sometimes associated with vesicles and vacuoles. Chitin or its oligomers were located with wheat germ agglutinin in the bud scars but also in the cell wall and the cytoplasm near the plasmalemma. Both mannan and chitin or its oligomers were found in the forming septum and are synthesized within the cytoplasm. The gold method was also suitable for marking mannan and chitin simultaneously.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 15 (1977), S. 1015-1021 
    ISSN: 1573-4927
    Keywords: Saccharomyces cerevisiae ; enzymes ; polymorphisms ; competition ; variable environments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Competition experiments were carried out under varying exogenic and endogenic conditions. The genotypes were marked by combinations of two esterase loci, each with two alleles. When genotypes of the line W7 were used, there was no demonstrable influence of the gene blocks marked by the Est-1 locus on the competitive ability at temperatures of 21 and 29 C. However, genotypes carrying the fast allele of the Est-2 locus were favored. At 38 C, the outcome of the competition was reversed. The defined gene blocks showed different effects when interacting with different genetic backgrounds (line M7). Genotypes marked by the slow allele of the Est-2 locus were now favored (21 and 29 C), and even the gene blocks marked by the alleles of the Est-1 locus influenced the genotypes' competitive abilities. Again, the results were partly reversed at 38 C. The results are discussed with regard to the importance of enzyme variants for the genotypic selection value.
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