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  • Books
  • Articles  (13)
  • isozymes  (10)
  • Chemical Engineering
  • Electronic structure and strongly correlated systems
  • Saccharomyces cerevisiae
  • Springer  (13)
  • 1975-1979  (13)
  • 1975  (13)
Collection
  • Books
  • Articles  (13)
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Keywords
Publisher
Years
  • 1975-1979  (13)
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    The isolation and characterization of peroxisomes (microbodies) from baker's yeast, Saccharomyces cerevisiae (1975)
    Springer
    Archives of microbiology 105 (1975), S. 187-192 
    Details
    ISSN: 1432-072X
    Keywords: Peroxisomes (microbodies) ; Saccharomyces cerevisiae ; Catalase ; Urate oxidase ; Glyoxylate cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Peroxisomes were isolated from derepressed (lactose grown) Saccharomyces cerevisiae cells following homogenization with a “Merkenschlager” cell mill (at 0°C using glass beads). Catalase and urate oxidase, along with low activities of d-amino acid oxidase and l-α-hydroxyacid oxidase (glycollate oxidase), were associated with the peroxisomes. No catalase activity was present in glucose repressed cells. When protoplasts prepared from derepressed cells were used for peroxisome isolation, catalase activity was not sedimentable through gradients. Apparently peroxisomes were destroyed as the cells became fermentative during protoplast preparation. The distribution of glyoxylate cycle enzymes was examined. Isocitrate lyase was not sedimentable, suggesting that, if the enzyme is peroxisome-associated, it is either readily released or present in a labile second class of peroxisomes. Low activities of malate dehydrogenase and citrate synthetase were found in peroxisome fractions from gradients, but may represent mitochondrial contamination. Citrate synthetase was not found associated with a low-density particle as had been previously reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447136
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  • 2
    Electronic Resource
    Electronic Resource
    Dependence of the maximum temperature for growth of Saccharomyces cerevisiae on nutrient concentration (1975)
    Springer
    Archives of microbiology 104 (1975), S. 23-28 
    Details
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Yeast ; Chemostat ; Nutrient Concentration ; Thermal Death ; Thermal Association ; Optimum Temperature for Growth ; Maximum Temperature for Growth ; Microbial Ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Saccharomyces cerevisiae was grown in a chemostat under glucose limitation at three superoptimal temperatures. In each steady state the specific growth rate was the sum of the dilution rate and the specific death rate, exponential death concurring with exponential growth. The specific death rate was a function of the temperature while the specific growth rate was a function of both the temperature and the concentration of the limiting nutrient. Each superoptimal temperature was characterized by a critical glucose concentration below which net growth was not possible. The critical glucose concentration increased with the temperature. Consequently the maximum temperature for growth was a function of the concentration of the limiting nutrient and approached the optimum temperature for growth with decreasing glucose concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447295
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of aeration on the activity of gluconeogenetic enzymes in Saccharomyces cerevisiae growing under glucose limitation (1975)
    Springer
    Archives of microbiology 106 (1975), S. 271-273 
    Details
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Baker's yeast ; Gluconeogenetic enzymes ; Chemostat ; Oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. The effect of aeration on the key enzymes of gluconeogenesis was studied in baker's yeast (Saccharomyces cerevisiae) and in a nonrespiratory variant of S. cerevisiae grown under glucose limitation. 2. In baker's yeast phosphoenolpyruvate carboxykinase, hexosediphosphatase and isocitrate lyase were completely repressed under anaerobic conditions. Their repression could be partially reversed by using intense aeration. 3. In the nonrespiratory variant these enzymes were absent independently of aeration. 4. Pyruvate carboxylase of baker's yeast showed maximal activity under anaerobic conditions. In the nonrespiratory variant pyruvate carboxylase had low activity under both anaerobic and aerobic conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446534
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  • 4
    Electronic Resource
    Electronic Resource
    Variation in the isozymes of flax (Linum usitatissimum) genotrophs (1975)
    Springer
    Biochemical genetics 13 (1975), S. 687-697 
    Details
    ISSN: 1573-4927
    Keywords: flax ; environmental induction ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Peroxidase, esterase, and acid phosphatase isozymes of environmentally induced L and S genotrophs, nuclear DNA reversion types, and the original plastic (Pl) type of the flax variety Stormont Cirrus have been compared by polyacrylamide gel electrophoresis. Differences were observed in particular genotrophs in terms of peroxidase and acid phosphatase isozymes but not in the esterase isozymes. The isozyme pattern of a particular line was not correlated with the nuclear DNA amount. The relationship between the isozyme pattern and the phenotypes of the lines in which they are expressed is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484926
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  • 5
    Electronic Resource
    Electronic Resource
    Ontogeny of lactate dehydrogenase isozymes in chicken-quail hybrid embryos (1975)
    Springer
    Biochemical genetics 13 (1975), S. 7-18 
    Details
    ISSN: 1573-4927
    Keywords: chicken-quail hybrid ; lactate dehydrogenase ; isozymes ; starch gel electrophoresis ; allelic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ontogeny of lactate dehydrogenase (LDH) isozymes was examined in avian hybrids and compared with the isozyme patterns of the parental species. Hybrids were obtained by crossing female Japanese quail (Coturnix coturnix japonica) with male domestic chickens (Gallus gallus domesticus). By use of starch gel electrophoresis and an enzyme-specific stain, traces of embryonic paternally derived LDH were detected in unincubated hybrid eggs. It was concluded that the embryonic genes coding for the B subunits of LDH are activated during the hours between fertilization and oviposition. In early blastoderms, a great excess of maternally stored LDH is present. In the hybrid, the predominantly maternal pattern of isozymes shifts during embryogenesis to a predominantly paternal pattern. This was considered evidence for differential allelic regulation of LDH inactivation. A progressive trend toward the establishment of the adult distribution of isozymes in various tissues was also observed in the hybrid and quail, and found to be similar to chicken LDH isozyme ontogeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00486002
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  • 6
    Electronic Resource
    Electronic Resource
    Use of internal standards in electrophoretic surveys of enzyme polymorphism (1975)
    Springer
    Biochemical genetics 13 (1975), S. 833-847 
    Details
    ISSN: 1573-4927
    Keywords: electrophoresis ; enzyme polymorphism ; internal standards ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract General criteria are described for selecting internal standards for electrophoretic gels. Gel sieving behavior is used to characterize the size and charge of enzymes of interest and of putative standard proteins. For a variety of enzymes of the butterfly Colias meadii, hemoglobin and ferritin are shown to be effective standards.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484414
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  • 7
    Electronic Resource
    Electronic Resource
    A simple method for the quantitation of isozyme patterns (1975)
    Springer
    Biochemical genetics 13 (1975), S. 805-812 
    Details
    ISSN: 1573-4927
    Keywords: isozymes ; zone electrophoresis ; quantitative methods ; densitometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S=K/T, where K is 1.6×10−3 units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U=K (2) n−1, where n is the number of the slot producing the last visible band.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484412
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  • 8
    Electronic Resource
    Electronic Resource
    A study on lactate dehydrogenase isozymes in rat ova (1975)
    Springer
    Biochemical genetics 13 (1975), S. 65-72 
    Details
    ISSN: 1573-4927
    Keywords: lactate dehydrogenase ; isozymes ; immunohistochemistry ; rat ova
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The presence of subunits A and B has been demonstrated in mature rat ova by several means, including the immunohistochemical method of Coons, use of antisera against LDH-1 (B4) and LDH-5 (A4) isozymes, and polyacrylamide gel electrophoresis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00486007
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  • 9
    Electronic Resource
    Electronic Resource
    Esterase polymorphism in the corn earworm, Heliothis zea (Boddie): A survey of temporal and spatial allelic variation in natural populations (1975)
    Springer
    Biochemical genetics 13 (1975), S. 885-898 
    Details
    ISSN: 1573-4927
    Keywords: Heliothis zea ; esterases ; isozymes ; allelic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Allelic variation at the Est-II locus was investigated in 22 natural populations of Heliothis zea from maize, separated spatially and temporally. Of the four alleles observed, Est-II b was the predominant one in every population. The relative frequency of Est-II b ranged from 0.57 to 0.85, with a mean of 0.72±0.06, and proved to be remarkably stable in space and time. Temporal changes in Est-II allele frequencies were observed at two of the sampling localities; however, principal component analysis and multiple correlation of genetic and environmental components have provided persuasive evidence that abiotic environmental or geographic variation is not related to differences in Est-II allele frequencies. Possible factors responsible for the maintenance of this polymorphism are discussed and include possible effects of the chemical composition of host plants and pesticides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484418
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic aspects of quantitative variation in the carbonic anhydrases of the pig-tailed macaque, Macaca nemestrina. I. Response to thyroxine (1975)
    Springer
    Biochemical genetics 13 (1975), S. 673-686 
    Details
    ISSN: 1573-4927
    Keywords: primate carbonic anhydrases ; genetics ; thyroxine ; isozymes ; Macaca nemestrina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Effects of thyroxine on incorporation of l-serine-C14 into four carbonic anhydrase isozymes (CA II, CA Ia, CA Ib, CA Ic) and hemoglobin were quantified in reticulocytes of Macaca nemestrina in vitro. Response to thyroxine differed significantly between CA Ia and two allelic variants (CA Ib and CA Ic) and the nonallelic isozyme (CA II). The effects of thyroxine on serine incorporation into hemoglobin and three of the carbonic anhydrase isozymes were shown to be nonlinear with thyroxine concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484925
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  • 11
    Electronic Resource
    Electronic Resource
    A simple method of choosing the sample volume for ultramicroelectrophoresis of proteins (1975)
    Springer
    Bulletin of experimental biology and medicine 80 (1975), S. 1130-1132 
    Details
    ISSN: 1573-8221
    Keywords: ultramicroelectrophoresis ; proteins ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Optimal values for the quantity of proteins in samples for ultramicroelectrophoretic fractionation in polyacrylamide gel and capillary tubes of different diameters enabling clear and highly reproducible results to be obtained by electrophoresis were established experimentally. A simple method of calculating the necessary sample volumes for protein solutions of different concentrations and capillary tubes of different diameters by means of a table and formula is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00799911
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  • 12
    Electronic Resource
    Electronic Resource
    Lactate dehydrogenase isozyme spectrum in mouse mammary gland tumors and metastases in the lungs (1975)
    Springer
    Bulletin of experimental biology and medicine 80 (1975), S. 1484-1486 
    Details
    ISSN: 1573-8221
    Keywords: lactate dehydrogenase ; isozymes ; mammary gland tumors ; metastases in the lung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The lactate dehydrogenase isozyme spectrum was investigated in the mammary glands, lungs, spontaneous mammary gland tumors, and metastases of tumors in the lungs of female mice of the high-cancer strain C3H previously having given birth to young. Differences in the isozyme spectrum of the metastases and tumors were reflected in the larger fractions migrating to the anode in the metastatic nodes. The presence of a metastasis in the lung was accompanied by a cathodal shift in the isozyme spectrum of the lung tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00842646
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  • 13
    Electronic Resource
    Electronic Resource
    Effect of atp and cocarboxylase on activity of malate dehydrogenase and its isozymes in blood serum and myocardium (1975)
    Springer
    Bulletin of experimental biology and medicine 80 (1975), S. 907-909 
    Details
    ISSN: 1573-8221
    Keywords: malate dehydrogenase ; isozymes ; blood serum ; heart ; hypoxia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Changes in the activity of malate dehydrogenase (MD) and its isozymes in the blood serum and heart muscle of rats were investigated during hypoxia and under the influence of ATP and cocarboxylase. An additional cathodic isozyme (MD4) appeared in the blood serum during hypoxia. Under the influence of ATP and cocarboxylase the metabolic changes in the blood and myocardium induced by hypoxia did not correlate fully.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00789267
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