ISSN:
1573-4927
Keywords:
isozymes
;
zone electrophoresis
;
quantitative methods
;
densitometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S=K/T, where K is 1.6×10−3 units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U=K (2) n−1, where n is the number of the slot producing the last visible band.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00484412
Permalink