Publikationsdatum:
2012-11-08
Beschreibung:
Tobacco ( Nicotiana tabacum ) is one of the most economically important nonfood crops, and flue-cured tobacco accounts for approximately 80% of world tobacco production. An extremely narrow genetic diversity in the tobacco pool has led to a low efficiency of PCR-based molecular markers (such as AFLP and SSR). Diversity Arrays Technology (DArT) is a high-throughput hybridisation-based marker system that has been developed in many plants including wheat, which, like tobacco, has a complex genome. In this study, we developed a tobacco DArT chip that included 7680 representative sequence tags based on typical tobacco accessions. The 1076 DArT markers of flue-cured tobacco were identified and most (82.1%) of their polymorphism information contents (PICs) were greater than 0.4. An integrated linkage map that included 851 markers (238 DArT and 613 SSR), which is the highest density map of flue-cured tobacco to date, was constructed. This chip-based DArT system provides an alternative in high-throughput marker genotyping for tobacco. Content Type Journal Article Category Article Pages 1-8 DOI 10.1007/s11434-012-5453-z Authors XiuPing Lu, Department of Agronomy, Zhejiang University, Hangzhou, 310058 China YiJie Gui, Department of Agronomy, Zhejiang University, Hangzhou, 310058 China BingGuang Xiao, Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Center at Yunnan, Yuxi, 653100 China YongPing Li, Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Center at Yunnan, Yuxi, 653100 China ZhiJun Tong, Department of Agronomy, Zhejiang University, Hangzhou, 310058 China Yun Liu, Department of Agronomy, Zhejiang University, Hangzhou, 310058 China XueFei Bai, Department of Agronomy, Zhejiang University, Hangzhou, 310058 China WeiRen Wu, Department of Agronomy, Zhejiang University, Hangzhou, 310058 China Ling Xia, DArT P/L, PO Box 7141, Yarralumla, Canberra, ACT 2600, Australia Eric Huttner, DArT P/L, PO Box 7141, Yarralumla, Canberra, ACT 2600, Australia Adrzej Kilian, DArT P/L, PO Box 7141, Yarralumla, Canberra, ACT 2600, Australia LongJiang Fan, Department of Agronomy, Zhejiang University, Hangzhou, 310058 China Journal Chinese Science Bulletin Online ISSN 1861-9541 Print ISSN 1001-6538
Print ISSN:
1001-6538
Digitale ISSN:
1861-9541
Thema:
Allgemeine Naturwissenschaft
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