Publication Date:
2018-03-06
Description:
Interaction of sugar binding proteins–galectins, with glycoconjugates is considered relevant for various reproductive processes. Galectin-1 (gal-1) is a molecule involved in trophoblast cell invasion, which is accomplished through interaction with cell surface and/or extracellular matrix glycoproteins. A possibility of interaction of endogenous gal-1 and trophoblast β 1 integrins, both previously shown relevant for trophoblast invasion, was investigated. Confocal microscopy showed overlap in gal-1 and β 1 integrin localization at the plasma membrane of isolated cytotrophoblast, HTR-8/SVneo extravillous trophoblast cell line and JAr choriocarcinoma cells. Immunoprecipitation confirmed an interaction of gal-1 with integrin β 1 , but not with α 1 or α 5 integrin subunits. Nondenaturing electrophoresis and subcellular fractionation suggested association of gal-1 with β 1 integrin in intracellular and plasma membrane compartments of HTR-8/SVneo cells. Gal-1/β 1 integrin complex was sensitive to chemical and enzyme treatments, indicating carbohydrate dependent interaction. Down-regulation of gal-1 by siRNA, however, had no effect on level or distribution of β 1 integrin, as determined by qPCR and flow cytometry. These results suggest complex lectin type interaction of gal-1 with β 1 integrin at the trophoblast cell membrane, which could influence trophoblast cell adhesion, migration and invasion.
Print ISSN:
0021-924X
Electronic ISSN:
1756-2651
Topics:
Biology
,
Chemistry and Pharmacology
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