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  • 1
    Publikationsdatum: 2012-02-17
    Beschreibung: Rosen, S., Engås, A., Fernö, A., and Jörgensen, T. 2012. The reactions of shoaling adult cod to a pelagic trawl: implications for commercial trawling. – ICES Journal of Marine Science, 69: 303–312. The reactions of shoaling adult Atlantic cod to a pelagic trawl were measured during fishing off the north coast of Norway. Cod remaining in the trawl track dived at rates as fast as 0.35 m s –1 following vessel passage and swam away from the vessel, in the direction of the approaching trawl, at an average rate of 0.6 m s –1 . They did not attempt to swim ahead of the trawl as documented previously, but passed into the lower half of the trawl entrance and swam slowly in the direction of trawling at a rate of 0.2–0.5 m s –1 as the trawl's greater speed through water carried them deeper into the trawl. Shoals compressed vertically once inside the trawl, suggesting that packing density increased at least fourfold. Fish remained in the lower part of the trawl as they moved through its tapered section towards the codend, with little to no clearance above the bottom panel, but significant clearance beneath the top panel. Catches were sufficient to support commercial harvest, and the behaviour observed suggests that changes in trawl design and fishing strategy might improve fuel economy and species selectivity.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2012-02-17
    Beschreibung: Shackell, N. L., Bundy, A., Nye, J. A., and Link, J. S. 2012. Common large-scale responses to climate and fishing across Northwest Atlantic ecosystems. – ICES Journal of Marine Science, 69: 151–152. Investigating whether there were common biological responses to climate and fishing across seven Northwest Atlantic ecosystems, a minimum/maximum autocorrelation factor analysis of biological indicators for each region revealed a common primary multivariate trend of a rapid change during the 1980s and early 1990s. There was a strong common pattern in the biological indicators responsible for the primary multivariate temporal trend in the five more northerly regions: an increase in the abundance of phytoplankton, an increase in biomass at mid-trophic levels, and a decline in predatory groundfish size. The common associations between patterns and drivers were fishing indices and the Atlantic Multidecadal Oscillation, but all associations weakened when co-varying drivers were held constant. The results are consistent with known long-term effects of intense fishing, such as a decline in average fish size and changes in species composition. Less fishing pressure has allowed some regions to recover to former predatory biomass levels since the late 1990s, but the bulk of the biomass consists of fewer species. However, fishing was not the only driver, and a more mechanistic understanding of how the climate affects lower trophic levels is needed to contextualize climate effects in heavily fished ecosystems.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2012-02-17
    Beschreibung: Deporte, N., Ulrich, C., Mahévas, S., Demanèche, S., and Bastardie, F. 2012. Regional métier definition: a comparative investigation of statistical methods using a workflow applied to international otter trawl fisheries in the North Sea. – ICES Journal of Marine Science, 69: 331–342. The European Common Fisheries Policy recognizes the importance of accounting for heterogeneity in fishing practices, and métier-based sampling is now at the core of the EU Data Collection Framework. The implementation of such an approach would require Member States to agree on the standard regional métier definitions and on practical rules to categorize logbook records into métiers. Several alternative approaches have been used in the past to categorize landings profiles, but no consensus has yet emerged. A generic open-source workflow is developed to test and compare a selection of methods, including principal components analysis (PCA), hierarchical agglomerative clustering (HAC), K -means, and Clustering LARge Applications (CLARA), and to provide simple allocation rules. This workflow is applied to a unique regional dataset consisting of bottom-trawl logbooks of five North Sea countries. No method proved to be infallible, but combining PCA with either CLARA or HAC performed best. For 2008, a hierarchical classification with 14 species assemblages is proposed. Discriminant analysis proved more robust than simple ordination methods for allocating a new logbook record into an existing métier. The whole approach is directly operational and could contribute to defining more objective and consistent métiers across European fisheries.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2012-02-17
    Beschreibung: Greenstreet, S. P. R., Rogers, S. I., Rice, J. C., Piet, G. J., Guirey, E. J., Fraser, H. M., and Fryer, R. J. 2012. A reassessment of trends in the North Sea Large Fish Indicator and a re-evaluation of earlier conclusions. – ICES Journal of Marine Science, 69: 343–345. Previous analysis of the Large Fish Indicator, the basis for the North Sea "fish communities" EcoQO, suggested a strong recovery between 2001 and 2008. Discovery of a mistake in this earlier analysis now suggests that this recovery is not as strong as first thought, but reanalysis of the corrected data still supports the majority of conclusions drawn in the original paper.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2012-02-17
    Beschreibung: Xie, Y., Michielsens, C. G. J., and Martens, F. J. 2012. Classification of fish and non-fish acoustic tracks using discriminant function analysis. – ICES Journal of Marine Science, 69: 313–322. Hydroacoustic data acquired for estimating fish populations contain information on both fish and non-fish targets, so sonar technicians traditionally rely on their knowledge of fish behaviour and experience with hydroacoustics to remove non-fish targets from the hydroacoustic data. This process is often labour-intensive and time-consuming, making real-time assessment of fish populations difficult. Simple solutions are not always available for all circumstances. However, the split-beam sonar data collected in the lower Fraser River, British Columbia, Canada, showed distinct signatures between actively swimming fish and non-fish objects such as drifting debris, surface bubbles, and stationary objects in the water column and off the river bottom. Acoustic tracks of fish and non-fish targets were characterized by differentiable statistical patterns that were amenable to discriminant function analysis (DFA). An application of DFA to segregate fish and non-fish targets detected by a split-beam sonar system in the lower Fraser River is presented, characteristics of user-identified fish and non-fish acoustic tracks being utilized as learning samples for the DFA. Also, a method to rank the discriminating power of individual variables is presented, providing guidance for constructing efficient and effective discriminant functions with variables that offer high discriminating power. The DFA yielded classification accuracies of 96% for fish and 91% for non-fish tracks and reduced the manual sorting time by 50–75%.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-02-17
    Beschreibung: Rey, F. 2012. Declining silicate concentrations in the Norwegian and Barents Seas. – ICES Journal of Marine Science, 69: 208–212. Since 1990, a decline in silicate concentrations together with increasing salinities has been observed in the Atlantic water of the Norwegian and Barents Seas. This decline in silicate has been found to be related to the relative proportion in which eastern and western source water masses from the northeastern North Atlantic enter the Norwegian Sea.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Publikationsdatum: 2012-02-17
    Beschreibung: Zhou, S., Milton, D. A., and Fry, G. C. 2012. Integrated risk analysis for rare marine species impacted by fishing: sustainability assessment and population trend modelling. – ICES Journal of Marine Science, 69: 271–280. Risk assessment and conservation management of rare species are challenging due to a lack of data. We developed an integrated risk assessment approach to assess human impact on population sustainability of rare species. The approach involved two components: a quantitative sustainability assessment coupled with modelling trends in relative abundance. Both components took nil catches into account through zero-inflated statistical distributions that simultaneously modelled the zero and non-zero catches separately in submodels. The sustainability assessment used detection–non-detection data for population estimation and linked sustainability to easily collected life-history traits. This component provides an assessment of population sustainability at one point in time. The trend modelling applied zero-inflated negative binomial models to temporal trends in density and dispersion of species. It provided a complement to the static sustainability assessment. We applied this integrated approach to assess the risk to 14 species of rare, protected sea snakes incidentally caught in the Australian Northern Prawn Fishery. This approach can be applicable for risk assessment of many species with limited abundance data, a large number of absences and some presence–absence information only.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-02-17
    Beschreibung: Frid, C. L. J., and Paramor, O. A. L. 2012. Feeding the world: what role for fisheries? – ICES Journal of Marine Science, 69: 145–150. Fisheries (wild capture and aquaculture) deliver more than 110 million tonnes of food and around 15% of the dietary protein to the 7 billion people currently living on the planet. With the global population expected to peak at 9 billion by 2050, and 〉80% of global fish stocks currently fully or overexploited (and aquaculture is at least in part dependent on capture fisheries), the contribution of fisheries looks set to decline. The challenge is therefore determining how better management, an ecosystem perspective, and more efficient utilization of fisheries waste can support fisheries products continuing to contribute significantly to "feeding the world" up to and beyond the population peak.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2012-02-17
    Beschreibung: Boström, M. K., Östman, Ö., Bergenius, M. A. J., and Lunneryd, S-G. 2012. Cormorant diet in relation to temporal changes in fish communities. – ICES Journal of Marine Science, 69: 175–183. The stomach contents of 229 great cormorants ( Phalacrocorax carbo sinensis ) shot between March and October 2009 along the Swedish east coast were analysed for differences in diet between gender, age, and breeding phase. Sticklebacks ( Gasterosteus aculeatus and Pungitius pungitius ) were the most common prey, followed by eelpout ( Zoarces viviparus ), herring ( Clupea harengus membras ), and cyprinids (Cyprinidae). Diet did not differ between age and gender, but changed over the breeding season. The different phases explained around 10% of the total variation in stomach content between cormorants, suggesting no major shift in diet over the breeding season. The diet of cormorants in 2009 was compared with the results of a study conducted in the same area in 1992. There were evident changes in the diet between 1992 and 2009, with less perch ( Perca fluviatilis ) and cyprinids and more eelpout and herring in 2009. This change in diet could partly be related to changes in the fish community. The seasonal changes in diet composition of whole stomachs were less notable than in many previous studies, but long-term changes in the fish communities may induce changes in cormorant diet. It is clearly important to use stomach contents in areas with many small fish species for a comprehensive assessment of cormorant diet.
    Print ISSN: 1054-3139
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Publikationsdatum: 2012-02-17
    Beschreibung: Cañás, L., Stransky, C., Schlickeisen, J., Sampedro, M. P., and Fariña, A. C. 2012. Use of the otolith shape analysis in stock identification of anglerfish ( Lophius piscatorius ) in the Northeast Atlantic. – ICES Journal of Marine Science, 69: 250–256. The geographical variability in the anglerfish Lophius piscatorius otolith shape was investigated to examine biological evidence for the current separation into Northern and Southern stocks of L. piscatorius established in the Southwest European waters (Southern Shelf area) by the International Council for the Exploration of the Sea (ICES). In all, 629 otoliths of L. piscatorius (12–90 cm total length) from commercial fishing and research surveys were collected in two consecutive years, 2008 and 2009. The otolith shape was compared between stocks for differences in univariate descriptors using non-parametric analysis of covariance, as well as elliptical Fourier shape descriptors, through a metric multidimensional scaling and a linear discriminant analysis (LDA). The differences found in the otolith shape between Northern and Southern stocks were not large enough to hold the current status of L. piscatorius regarding stock definition in the Southern Shelf area, although homogeneous mixing between both stocks is not expected as there were significant differences between three univariate descriptors and the LDA was significant. These results are in agreement with the previous L. piscatorius stock identification studies (genetics, body morphology, and migratory behaviour), which do not support the current separation of these stocks but found substructures within the area.
    Print ISSN: 1054-3139
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-12-22
    Beschreibung: Angiogenesis, a formation of neovessels, is regulated by the local balance between angiogenesis stimulators and inhibitors. A number of such endogenous regulators of angiogenesis have been found in the body. Recently, vasohibin-1 (VASH1) was isolated as a negative feedback regulator of angiogenesis produced by endothelial cells (ECs) and subsequently vasohibin-2 (VASH2) as a homologue of VASH1. It was then explored that VASH1 is expressed in ECs to terminate angiogenesis, whereas VASH2 is expressed in cells other than ECs to promote angiogenesis in the mouse model of angiogenesis. This review will focus on the vasohibin family members, which are novel regulators of angiogenesis.
    Print ISSN: 0021-924X
    Digitale ISSN: 1756-2651
    Thema: Biologie , Chemie und Pharmazie
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Publikationsdatum: 2012-12-22
    Beschreibung: Tertiary dentin is deposited inside teeth after various stimuli and serves as a major defensive wall to preserve pulp cells. However, the molecular mechanisms of the activation of quiescent odontoblasts, immature pulp cells and tertiary dentin formation are still unclear. Therefore, we performed a comprehensive gene expression analysis of pulp cells after cavity preparation of 9-week-old rat molars to clarify the critical molecules in tertiary dentinogenesis. As a result, mRNA expression of various molecules was up- or down-regulated. Notably, several members of the matrix metalloprotease family and their endogenous inhibitors were up-regulated after cavity preparation. In situ hybridization showed that tissue inhibitor of metalloprotease 1 ( Timp1 ) was widely and continuously distributed in the pulp beneath the cavity in vivo . We also observed accumulation of β-catenin in the pulp cells beneath the cavity by fluorescence immunohistochemistry. Furthermore, Timp1 transcription was repressed by a dominant-negative TCF4 in immature undifferentiated mesenchymal cells, but not altered in mature odontoblast-like cells. These results indicate that cavity preparation may activate the Wnt/β-catenin pathway and the Wnt/β-catenin pathway and Timp1 may be correlatively involved in pulp repair. Timp1 might play crucial roles in reactivation of immature pulp cells for tertiary dentinogenesis.
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    Thema: Biologie , Chemie und Pharmazie
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-12-22
    Beschreibung: The activity of biological molecules is often affected by their phosphorylation state. Regulatory phosphorylation operates as a binary switch and is usually controlled by counteracting kinases and phosphatases. However, phosphatidylinositol (PtdIns) has three phosphorylation sites on its inositol ring. The phosphorylation status of PtdIns is controlled by multiple kinases and phosphatases with distinct substrate specificities, serving as a ‘lipid code’ or ‘phosphoinositide code’. Class I phosphoinositide 3-kinase (PI3K) converts PtdIns(4,5)P 2 to PtdIns(3,4,5)P 3 , which plays a pivotal role in signals controlling glucose uptake, cytoskeletal reorganization, cell proliferation and apoptosis. PI3K is pro-oncogenic, whereas phosphoinositide phosphatases that degrade PtdIns(3,4,5)P 3 are not always anti-oncogenic. Recent studies have revealed the unique characteristics of phosphoinositide 5-phosphatases.
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    Thema: Biologie , Chemie und Pharmazie
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    Publikationsdatum: 2012-12-22
    Beschreibung: Vascular endothelial growth factors (VEGFs) belong to the platelet-derived growth factor supergene family, and they play central roles in the regulation of angiogenesis and lymphangiogenesis. VEGF-A, the major factor for angiogenesis, binds to two tyrosine kinase (TK) receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1), and regulates endothelial cell proliferation, migration, vascular permeability, secretion and other endothelial functions. VEGFR-2 exhibits a strong TK activity towards pro-angiogenic signals, whereas the soluble VEGFR-1 (sFlt-1) functions as an endogenous VEGF inhibitor. sFlt-1 is abnormally overexpressed in the placenta of preeclampsia patients, resulting in the major symptoms of the disease due to abnormal trapping of VEGFs. The VEGF-VEGFR system is crucial for tumour angiogenesis, and anti-VEGF-VEGFR molecules are now widely used in the clinical field to treat cancer patients. The efficacy of these molecules in prolonging the overall survival of patients has been established; however, some cancers do not respond well and reduced tumour sensitivity to anti-VEGF signals may occur after long-term treatment. The molecular basis of tumour refractoriness should be determined to improve anti-angiogenic therapy.
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    Thema: Biologie , Chemie und Pharmazie
    Standort Signatur Erwartet Verfügbarkeit
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  • 15
    Publikationsdatum: 2012-12-22
    Beschreibung: The actual levels of steroid hormones in organs are vital for endocrine, reproductive and neuronal health and disorders. We developed an accurate method to determine the levels of steroid hormones and steroid conjugates in various organs by an efficient preparation using a solid-phase-extraction cartridge. Each steroid was identified by the precursor ion spectra using liquid chromatography–electrospray ionization time-of-flight mass spectrometry, and the respective steroids were quantitatively analysed in the selected reaction monitoring mode by liquid chromatograph-mass spectrometry/mass spectrometry (LC-MS/MS). The data showed that significant levels of testosterone, corticosterone and precursors of both hormones were detected in all organs except liver. The glucuronide conjugates of steroid hormones and the precursors were detected in all organs except liver, but sulfate conjugates of these steroids were observed only in the target organs of the hormones and kidney. Interestingly, these steroids and the conjugates were not observed in the liver except pregnenolone. In conclusion, an accurate determination of tissue steroids was developed using LC-MS analysis. Biosynthesis of steroid hormones from the precursors was estimated even in the target organs, and the delivery of these steroid conjugates was also suggested via the circulation without any significant hepatic participation.
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    Thema: Biologie , Chemie und Pharmazie
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  • 16
    Publikationsdatum: 2012-12-22
    Beschreibung: Papain-like cysteine protease activity that shows a unique transient expression profile in cotyledons of daikon radish during germination was detected. The enzyme showed a distinct elution pattern on DEAE-cellulose compared with cathepsin B-like and Responsive to dessication-21 cysteine protease. Although this activity was not detected in seed prior to imbibition, the activity increased markedly and reached a maximum at 2 days after imbibition and then decreased rapidly and completely disappeared after 5 days. Using cystatin-Sepharose, the 26 kDa cysteine protease (DRCP26) was isolated from cotyledons at 2 days after imbibition. The deduced amino acid sequence from the cDNA nucleotide sequence indicated that DRCP26 is an orthologue of Arabidopsis unidentified protein, germination-specific cysteine protease-1, belonging to the C1 family of cysteine protease predicted from genetic information. In an effort to characterize the enzymatic properties of DRCP26, the enzyme was purified to homogeneity from cotyledons at 48 h after imbibition. The best synthetic substrate for the enzyme was carbobenzoxy-Phe-Arg-4-methylcoumaryl-7-amide. All model peptides were digested to small peptides by the enzyme, suggesting that DRCP26 possesses broad cleavage specificity. These results indicated that DRCP26 plays a role in the mobilization of storage proteins in the early phase of seed germination.
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    Thema: Biologie , Chemie und Pharmazie
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  • 17
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-12-12
    Beschreibung: Corten, A. 2013. Recruitment depressions in North Sea herring. – ICES Journal of Marine Science, 70:1–15. Two periods of sustained low recruitment have been observed in North Sea herring in recent history; one from 1971 to 1979 and one that started in 2002 and continued up until the most recent year for which information is available (2010). This paper compares both periods of recruitment depression and considers the possible causes for each of them. The first depression (1971–1979) has been commonly ascribed to insufficient egg production by the depleted parent stock. However, recruitment to the central and northern populations was probably also affected by an environmental factor. There are indications of a reduced Atlantic inflow into the northwestern North Sea at the time of the depression. This could have affected survival of the larvae by slowing down their transport to the nursery areas in the southeastern North Sea. For the second period (2002–2010), the low recruitment has been ascribed to an increase in temperature and/or a regime shift in the ecosystem. An alternative explanation could be predation by the large stock of adult herring on its own larvae.
    Print ISSN: 1054-3139
    Digitale ISSN: 1095-9289
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 18
    Publikationsdatum: 2012-12-12
    Beschreibung: Punt, A. E., Huang, T., and Maunder, M. N. 2013. Review of integrated size-structured models for stock assessment of hard-to-age crustacean and mollusc species. – ICES Journal of Marine Science, 70:16–33. Crustaceans and molluscs such as crabs, rock lobsters, prawns, abalone, and oysters constitute large and valuable fisheries. However, assessments of these species are hampered because they cannot be production aged, in contrast to many teleosts. The major data sources for these species, in addition to catch and abundance index data, are the size compositions of the catches and of any fishery-independent indices. Assessments of such species have been conducted using age-based methods of stock assessment, as well as surplus production models. However, size-structured methods are now preferred because they can make full use of size-composition data, are able to integrate multiple sources of data, and produce the types of outputs which are needed for management purposes. An advantage of size-based models over age-based models is that all processes can be size-based, and these processes can modify the (unmodelled) size-at-age distribution. We review these methods, highlighting the choices that need to be made when developing integrated size-structured stock assessments, the data sources which are typically available and how they are used for parameter estimation, and contrast a number of such assessments worldwide.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 19
    Publikationsdatum: 2012-12-12
    Beschreibung: De Robertis, A. and Handegard, N. O. 2013. Fish avoidance of research vessels and the efficacy of noise-reduced vessels: a review. – ICES Journal of Marine Science, 70:34–45. It has long been recognized that fish can avoid approaching vessels and that these behaviours can bias fishery surveys. Underwater noise is considered the primary stimulus, and standards for research vessel noise have been established to minimize fish reactions. We review the literature on fish reactions to vessels appearing since these recommendations were made, focusing on acoustic surveys, and compare how fish react to noise-reduced and conventional vessels. Reactions to approaching vessels are variable and difficult to predict. However, the behaviour can bias acoustic abundance measurements, and should be considered when performing acoustic surveys. The few comparisons of acoustic abundance measurements from noise-reduced and conventional vessels are contradictory, but demonstrate that the sound pressure level, on which the noise-reduction criterion is based, is insufficient to explain how fish react to survey vessels. Further research is needed to identify the stimuli fish perceive from approaching vessels and the factors affecting whether fish perceiving these stimuli will react before further recommendations to reduce vessel-avoidance reactions can be made. In the interim, measurement of the biases introduced by fish avoidance reactions during surveys, and timing of surveys when fish are in a less reactive state, may reduce errors introduced by vessel avoidance.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 20
    Publikationsdatum: 2012-12-12
    Beschreibung: Xavier, J. C., Cherel, Y., Roberts, J., and Piatkowski, U. 2013. How do cephalopods become available to seabirds: can fish gut contents from tuna fishing vessels be a major food source of deep-dwelling cephalopods? – ICES Journal of Marine Science, 70:46–49. Cephalopods are important prey for numerous seabird species. However, the physical mechanisms by which cephalopods (particularly species considered as deep-dwelling) become available to seabirds are poorly understood, and it has recently been suggested that the discarded stomachs of gutted fish captured by tuna longliners can be a major source of deep-dwelling species. Here, we identify some deep-dwelling cephalopods that appear in the diet of seabirds, review the current knowledge of their vertical distribution, and compare the stomach contents of commercially captured tuna with those of seabirds foraging in the same area. The limited available information leads us to conclude that tuna longliners are unlikely to be a major source of deep-dwelling cephalopods for seabirds. However, much more information is required on the ecology of seabird prey, particularly commercially unexploited cephalopod species, which may be obtained from scientific cruises devoted to cephalopod biological research. In addition multispecies/foodweb modelling studies may be required to explore potential interactions between seabirds, their predators and prey, and commercial fishing operations.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 21
    Publikationsdatum: 2012-12-12
    Beschreibung: Karlson, S., Michalsen, K., and Folkvord, A. 2013. Age determination of Atlantic halibut ( Hippoglossus hippoglossus L.) along the coast of Norway: status and improvements. – ICES Journal of Marine Science, 70:50–55. This study re-evaluates the current ageing methodology for the Atlantic halibut, Hippoglossus hippoglossus . The traditional method is through surface readings of otoliths, but, based on new experiments with different preparation treatments and techniques, a more accurate and cost-efficient procedure for the age determination of Atlantic halibut is proposed.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 22
    facet.materialart.
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    Oxford University Press
    Publikationsdatum: 2012-12-12
    Beschreibung: Waldo, S., and Paulrud, A. 2013. ITQs in Swedish demersal fisheries. – ICES Journal of Marine Science, 70:68–77. Individual transferable quota (ITQ) is a management measure that is widely discussed, not least in the reform of the European Common Fisheries Policy. While the system is expected to reduce overcapacity and improve economic performance, questions are raised concerning the future of small-scale fisheries. This paper uses a model for Swedish fisheries (the Swedish Resource Rent Model for the Commercial Fisheries, SRRMCF) where the economics and fleet structure in a potential Swedish ITQ-system are analysed. The model is an optimization model based on linear programming and data from the European Union's data collection framework. The modelling approach can readily be used by other member states. Three main conclusions can be drawn on how ITQs will affect fisheries: The fishing fleet measured in number of vessels will decrease by approximately 30–50%. Profitability will increase so that the fishing industry will be able to provide competitive wages and make a financial contribution to fisheries management. The system can be designed so that small-scale fisheries are not disadvantaged.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 23
    Publikationsdatum: 2012-12-12
    Beschreibung: Tzanatos, E., Castro, J., Forcada, A., Matic-Skoko, S., Gaspar, M., and Koutsikopoulos, C. 2013. A Métier-Sustainability-Index (MSI25) to evaluate fisheries components: assessment of cases from data-poor fisheries from southern Europe. – ICES Journal of Marine Science, 70:78–98. The present work presents an integrated comparative assessment of the sustainability of fishing tactics or métiers (combinations of area, season, fishing gear, and target species). An index, which includes biological, conservation, socioeconomic, and management criteria, was created for this purpose: the "Métier Sustainability Index" (MSI25). It was designed using the traffic lights approach and comprises 25 indicators that refer to the "health" of the fisheries. Additionally, the MSI25 provides an indication of the "certainty" of that information with regard to the data type/heterogeneity. Subsequently, the index was tested using data from six case studies (81 métiers) from the Mediterranean and the Atlantic. The index showed that 63.0% of the métiers were "sustainable", 21.0% were "unsustainable" and 16.0% were "under pressure"; 59.3% of characterizations were considered as "certain". The index evaluation showed significant differences between areas and gears. This paper considers the characteristics of the datasets used, the case management status, and the applicability of the index in improving the management of specific fisheries. Overall, the MSI25 was found to be a useful tool for the evaluation and management of highly heterogeneous fisheries and data from various sources and types.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 24
    Publikationsdatum: 2012-12-12
    Beschreibung: Favaro, B., Duff, S. D., and Côté, I. M. 2013. A trap with a twist: evaluating a bycatch reduction device to prevent rockfish capture in crustacean traps. – ICES Journal of Marine Science, 70:114–122. Bycatch, or the incidental capture of non-target species, occurs in fisheries around the world, with often detrimental ecological consequences. Bycatch reduction devices (BRDs) that increase catch specificity have been used successfully in some fisheries, and the development of such devices remains an important component of the global effort to reduce bycatch rates. We tested novel devices designed to exclude juvenile rockfish ( Sebastes spp.) from traps used to catch spot prawns ( Pandalus platyceros ), a commercially important species in British Columbia, Canada. The devices included reductions in trap opening sizes and novel bent-tunnel openings. Reducing trap opening size did not affect bycatch rates of rockfish or other non-target fish species. In contrast, bent-tunnel BRDs eliminated rockfish bycatch, and two of the bent-tunnel variants also excluded other fish species. However, prawn catch rates were reduced in all modified gear, and large prawns were often excluded more than small prawns. Videos recorded in situ revealed that prawn attempts to enter traps took longer and were more likely to fail in BRD-equipped than in unmodified traps. We conclude that bent-tunnel BRDs have the potential to be useful, but improvements are needed to increase prawn catch to levels similar to that of unmodified traps.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 25
    Publikationsdatum: 2012-12-12
    Beschreibung: Alemany, D., Iribarne, O. O., and Acha, E. M. 2013. Effects of a large-scale and offshore marine protected area on the demersal fish assemblage in the Southwest Atlantic. – ICES Journal of Marine Science, 70:123–134. There are few extensive and offshore located marine protected areas (MPAs) in the world oceans and their performance is still being debated. We evaluated the effects of a large-scale offshore MPA located on the Southwest Atlantic Patagonian Shelf (43°S 63°W) on the demersal fish assemblage. Compliance of the Patagonian MPA was assessed by analysing eight years of satellite vessel monitoring system (VMS; 2000–2008) data, which showed compliance and fishing effort concentrated near the protection boundaries. MPA effects were studied by employing a five year database collected by a scientific research vessel in protected and fishing locations, before and after the MPA establishment. We assessed 152 scientific trawling stations using multivariate analysis of fish assemblage structure, fish abundance (discriminating target and non-target species), and mean size and proportion of juveniles of the target species (Argentine hake, Merluccius hubbsi ). The identified MPA effects were a trend towards increasing abundance of the demersal fish assemblage, the target and non-target fish species, and hake juvenile size, and a higher proportion of juveniles aged 2+ inside the MPA. These positive trends support the case for offshore, large-scale MPAs.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    Publikationsdatum: 2012-12-12
    Beschreibung: Zischke, M. T., Griffiths, S. P., Tibbetts, I. R., and Lester, R. J. G. 2013. Stock identification of wahoo ( Acanthocybium solandri ) in the Pacific and Indian Oceans using morphometrics and parasites. – ICES Journal of Marine Science, 70:164–172. The wahoo ( Acanthocybium solandri ) is an increasingly important by-product species of tropical pelagic fisheries worldwide. However, specific management of the species is currently hindered by a dearth of information on basic biology and stock structure. This study examined the stock structure of wahoo using morphometric characters and parasite fauna from fish collected in three regions of the western Pacific, and one region in each of the eastern Pacific and eastern Indian Oceans. Similar morphometric measurements and parasite abundance of wahoo collected off eastern Australia suggest they may form part of a single phenotypic stock in the western Pacific Ocean. Morphometric measurements and parasite fauna were significantly different among wahoo from the western Pacific and eastern Pacific Oceans, suggesting multiple discrete phenotypic stocks despite genetic homogeneity. Assessing fish from a range of regions throughout the Pacific Ocean may help discriminate stock boundaries in this region. Future research using complementary techniques, such as otolith microchemistry and genetic microsatellites, may improve our understanding of the global stock structure of wahoo to suitably inform regional fishery management organizations.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 27
    Publikationsdatum: 2012-12-12
    Beschreibung: Benoît, H. P., Plante, S., Kroiz, M., and Hurlbut, T. 2013. A comparative analysis of marine fish species susceptibilities to discard mortality: effects of environmental factors, individual traits, and phylogeny. – ICES Journal of Marine Science, 70:99–113. Determining the sustainability of fishing mortality for discards requires information on discard amounts as well as capture and release mortality rates. Formal estimates of these rates are costly and only available for a limited number of species and fisheries. In their absence, proxies for discard mortality could inform risk assessments of fishing mortality sustainability for discarded species. Here, time-to-mortality (TM) was assessed for 48 marine fish species exposed to air following capture during an annual multi-species bottom-trawl survey. Species-specific estimates of TM were related qualitatively to more formal estimates of discard mortality from commercial fisheries, confirming the use of TM as a proxy. The effects on TM of species and individual traits, phylogenetic similarity (proxy for traits not explicitly included in the analysis) and environmental factors related to capture were also assessed. Much of the observed individual variability was explained by intraspecific and interspecific positive relationships between body size and TM. Sedentary species and those lacking a gas bladder or deciduous scales had greater TM. Effects of phylogeny and capture depth and temperature were also found. This study demonstrates how reliable proxies of discard mortality rate can be readily obtained in the field or estimated from relevant covariates.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 28
    Publikationsdatum: 2012-12-12
    Beschreibung: Andersen, J. M., Wiersma, Y. F., Stenson, G. B., Hammill, M. O., Rosing-Asvid, A., and Skern-Maurizen, M. 2013. Habitat selection by hooded seals ( Cystophora cristata ) in the Northwest Atlantic Ocean. – ICES Journal of Marine Science, 70:173–185. We examined annual habitat use for 65 hooded seals (32 adult females, 17 adult males, and 16 juveniles) equipped with satellite relay data loggers (SRDLs) in spring or summer during five field seasons (2004–2008). A combined approach using first passage time (FPT) analysis and a generalized additive model (GAM) was applied to test for habitat selection, with a focus on environmental parameters of depth, slope, ice, sea surface temperature (SST), and chlorophyll. The models were run on adult males, adult females, and juveniles separately, and the results identified SST, depth, and chlorophyll as the most important factors influencing habitat selection across all categories. Furthermore, males and females preferred similar habitat conditions, but were separated geographically, and by depth, at various times of the year. Males appeared to be more localized in their habitat use patterns, focusing their search effort in areas of complex seabed relief such as Baffin Bay, Davis Strait, and the Flemish cap, while females concentrated their search effort along shelf areas (e.g. the Labrador shelf). These findings support our hypothesis that hooded seals prefer areas where topography and oceanographic processes create favourable foraging conditions.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 29
    Publikationsdatum: 2012-12-12
    Beschreibung: Dietz, R., Teilmann, J., Andersen S. M. Rigét, F., and Olsen, M. T. 2013. Movements and site fidelity of harbour seals ( Phoca vitulina ) in Kattegat, Denmark, with implications for the epidemiology of the phocine distemper virus. – ICES Journal of Marine Science, 70:186–195. Twenty-seven harbour seals were caught and tagged at the island of Anholt in central Kattegat, Denmark, the epicentre of the phocine distemper virus (PDV) outbreaks in 1988 and 2002 that killed 50–60% of the populations. The satellite tagging shows that harbour seals from Anholt moved widely across Kattegat with a maximum distance of 249 km from the tagging haul-out site. Overall, females travelled over a wider area compared with males [90% kernel home range (KHR) females, 5189 km 2 ; males, 3293 km 2 ). KHR calculated for yearlings (6414 km 2 ) is larger than for subadults (2534 km 2 ), which again is larger than for adult seals (1713 km 2 ), showing a strong site fidelity, indicating limited gene flow between haul-out sites. Distances moved and home range sizes increased across autumn, peaked in February–March, and decreased through spring. During the breeding season in spring, all seals were very stationary around Anholt. The onset of the PDV epizootics in 1988 and 2002 took place when the Anholt harbour seals congregate on the Island during April. Anholt seal were also documented to have contact with infected seal locations at Hesselø, Læsø, and the Swedish west coast, although this contact takes place during winter prior to the documented summer outbreaks.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 30
    Publikationsdatum: 2012-12-12
    Beschreibung: Stockwell, J. D., Weber, T. C., Baukus, A. J., and Jech, J. M. 2013. On the use of omnidirectional sonars and downwards-looking echosounders to assess pelagic fish distributions during and after midwater trawling. – ICES Journal of Marine Science, 70:196–203. Small pelagic fish can play an important role in the structure and function of ecosystems, and there is increasing interest in their non-market value. At the scale of fish aggregations, however, the impact of fishing has received relatively little attention, with most effort devoted to impacts of vessel and gear avoidance on stock size estimates. We used concurrent deployment of a downwards-looking echosounder (Simrad ES60 system) and an omnidirectional sonar (Simrad SP90 system) during commercial pairtrawling operations for Atlantic herring ( Clupea harengus ) in the Gulf of Maine to examine their potential for studying the impacts of fishing on herring aggregations. We compared a number of aggregation metrics to illustrate similarities and differences between the two systems, and then qualitatively examined their properties during and after pairtrawling events to illustrate potential applications. Our results suggest that using both downwards-looking and omnidirectional systems provides complementary information on fish aggregation metrics. Future applications of these systems in before–after–control-impact (BACI) designs may help inform management agencies when evaluating potential impacts of fishing at the time and space scales of pelagic fish aggregations.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 31
    Publikationsdatum: 2012-12-12
    Beschreibung: Smith, J. N., Ressler, P. H., and Warren, J. D. 2013. A distorted wave Born approximation target strength model for Bering Sea euphausiids. – ICES Journal of Marine Science, 70:204–214. Acoustic surveys monitor euphausiid populations in the Bering Sea because of their importance as prey for walleye pollock and other organisms. Various scattering models exist to convert acoustic backscatter data to estimates of euphausiid numerical density or biomass, but a target strength (TS) model specific to Bering Sea euphausiids has not been available. This study parameterized a distorted wave Born approximation (DWBA) scattering model using physical (length and body shape) and material (density contrast, g , and sound speed contrast, h ) properties measured from live euphausiids. All model parameters (length, shape, material properties, orientation) were evaluated for their effect on predicted TS. A polynomial function was used to describe animal shape and produced smaller TS estimates compared to a taper function, as is traditionally used in DWBA scattering models of euphausiids. Animal length was positively correlated with TS, but variations in other parameters (including material properties and orientation) also produced large changes in TS. Large differences in TS between estimates calculated using measured versus literature material property values caused large variations in acoustic estimates of euphausiid numerical densities (animals m –3 ) which emphasizes the importance of collecting site-specific g and h measurements when possible.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 32
    Publikationsdatum: 2012-12-12
    Beschreibung: Cadigan, N. G. 2013. Fitting a non-parametric stock–recruitment model in R that is useful for deriving MSY reference points and accounting for model uncertainty. – ICES Journal of Marine Science, 70:56–67. Modelling the relationship between parental stock size and subsequent recruitment of fish to a fishery is often required when deriving reference points, which are a fundamental component of fishery management. A non-parametric approach to estimate stock–recruitment relationships is illustrated using a simulated example and nine case studies. The approach preserves compensatory density dependence in which the recruitment rate monotonically decreases as stock size increases, which is a basic assumption of commonly used parametric stock–recruitment models. The implications of the non-parametric estimates on maximum sustainable yield (MSY) reference points are illustrated. The approach is used to provide non-parametric bootstrapped confidence intervals for reference points. The efficacy of the approach is investigated using simulations. The results demonstrate that the non-parametric approach can provide a more realistic estimation of the stock–recruitment relationship when informative data are available compared with common parametric models. Also, bootstrap confidence intervals for MSY reference points based on different parametric stock–recruitment models often do not overlap. The confidence intervals based on the non-parametric approach tend to be much wider, and reflect better uncertainty due to stock–recruit model choice.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 33
    Publikationsdatum: 2012-12-12
    Beschreibung: van Putten, I. E., Gorton, R. J., Fulton, E. A., and Thebaud, O. 2013. The role of behavioural flexibility in a whole of ecosystem model. – ICES Journal of Marine Science, 70:150–163. The predictive accuracy of complex fisheries models developed to anticipate the effects of changing fishery regulations appears to depend on a solid understanding of the processes and feedback systems linking biological and physical information to resource user. Many fisher decisions are modelled in the human component of the models, including inertia, or location choice flexibility. We unpack a whole of ecosystem system model and explore how location choice flexibility in fleet behaviour (sticking to the same seasonal and spatial distribution of fishing) affects outcomes such as catches and income levels and variability. Our analysis shows that the interpretation is not straightforward, and the relationship between behavioural flexibility and income level and income variability has to be considered in the context of three main fleet characteristics: profitability; how diversified the fleet is; and growing or declining target species biomass. We contend that making behavioural flexibility sensitive to the health of the stock and fleet profitability could potentially improve accuracy of large whole of ecosystem models such as Atlantis.
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  • 34
    Publikationsdatum: 2012-12-12
    Beschreibung: Bachiller, E. and Irigoien, X. 2013. Allometric relations and consequences for feeding in small pelagic fish in the Bay of Biscay. – ICES Journal of Marine Science, 70:232–243. The body size of fish is an important factor in determining their biology and ecology, as predators eat prey smaller than themselves. Predator mouth size restricts the availability of possible prey. In this paper we provide the allometric relationships of eight common, small pelagic fish species in the Bay of Biscay. In addition, we describe the predator-prey size ratios for different species, and we determine changes in their ratio-based trophic-niche breadth with increasing body size. Results suggest that gape size does not totally determine the predator-prey size ratio distribution, but predators use the entire available prey size range, including the smallest. As they grow they simply incorporate larger prey as their increased gape size permits. Accordingly, a large degree of overlap was found in the diet composition in terms of size and predator-prey ratios, even between fish of different sizes. Of the species studied, only horse mackerels seem to be clearly specialized in relatively large prey.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 35
    Publikationsdatum: 2012-12-12
    Beschreibung: Lassalle, G., Lobry, J., Le Loc'h, F., Mackinson, S., Sanchez, F., Tomczak, M. T., and Niquil, N. 2013. Ecosystem status and functioning: searching for rules of thumb using an intersite comparison of food-web models of Northeast Atlantic continental shelves. – ICES Journal of Marine Science, 70:135–149. This work aimed to provide a better understanding of how the structure and function of marine ecosystems and trophic control mechanisms influence their response to perturbations. Comparative analysis of Ecopath models of four Northeast Atlantic ecosystems was used to search for rules of thumb defining the similarities and differences between them. Ecosystem indicators, related to the ecology of species interactions, were derived from these models and compared. Two main questions were addressed. (i) What are the main energy pathways and mechanisms of control? (ii) Do these ecosystems exhibit the widespread and potentially stabilizing food-web structure such that top predators couple distinct energy pathways? A strong bentho-pelagic coupling operated over the Bay of Biscay Shelf, while energy reached higher trophic levels mostly through pelagic compartments, in northern areas. Zooplankton was demonstrated to be trophically important in all ecosystems, acting as a regulator of the abundance of small pelagic fish. A latitudinal pattern in flow control was highlighted by this analysis, with a significant contribution of top-down effect at higher latitudes. This top-down control of the Baltic Sea, combined with the fact that this ecosystem did not exhibit the potentially stabilizing two-channel structure, suggested a non-stable environment.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 36
    Publikationsdatum: 2012-12-12
    Beschreibung: Fernández, A., Graña, R., Mouriño-Carballido, B., Bode, A., Varela, M., Domínguez-Yanes, J. F., Escánez, J., de Armas, D., and Marañón, E. 2013. Community N 2 fixation and Trichodesmium spp. abundance along longitudinal gradients in the eastern subtropical North Atlantic. – ICES Journal of Marine Science, 70:223–231. We have determined planktonic community N 2 fixation, Trichodesmium abundance, the concentration and vertical diffusive flux of phosphate, and satellite-derived estimates of atmospheric concentration of dust along two longitudinal transects in the eastern subtropical North Atlantic during November 2007 and from April–May 2008. Trichodesmium abundance was particularly low (〈3 trichome l –1 ) during the spring 2008 cruise, when low sea surface temperatures were recorded and vertical stratification was less marked. However, community N 2 fixation was always measurable, albeit low compared with other regions of the tropical Atlantic. The average, vertically-integrated N 2 fixation rate was 1.20 ± 0.48 µmol N m –2 d –1 in autumn 2007 and 8.31 ± 3.31 µmol N m –2 d –1 in spring 2008. The comparison of these rates of diazotrophy with the observed Trichodesmium abundances suggests that other, presumably unicellular, diazotrophs must have contributed significantly to community N 2 fixation, at least during the spring 2008 cruise. Satellite data of atmospheric dust concentration suggested similar rates of atmospheric deposition during the two surveys. In contrast, vertical diffusive fluxes of phosphate were 5-fold higher in spring than in autumn (14.2 ± 12.1 µmol P m –2 d –1 and 2.8 ± 2.6 µmol P m –2 d –1 , respectively), which may have stimulated N 2 fixation. These findings agree with the growing view that N 2 fixation is a more widespread process than the distribution of Trichodesmium alone may suggest. Our data also suggest a role for phosphorus supply in controlling the local variability of diazotrophic activity in a region subject to relatively high atmospheric inputs of iron.
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  • 37
    Publikationsdatum: 2012-12-12
    Print ISSN: 1054-3139
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 38
    Publikationsdatum: 2012-12-12
    Beschreibung: Macaulay, G. J., Kloser, R. J., and Ryan, T. E. 2013. In situ target strength estimates of visually verified orange roughy. – ICES Journal of Marine Science, 70:215–222. The first estimates of orange roughy ( Hoplostethus atlanticus ) target strength at 38 and 120 kHz with visual verification were obtained from a self-contained echosounder and video camera system affixed to a demersal trawl towed through dense aggregations of spawning orange roughy. Mean target strength estimates were obtained from 24 tracks of orange roughy containing 83 echoes. The mean target strength at 38 kHz was –52.0 dB with a 95% confidence interval of –53.3 to –50.9 dB for fish with a mean length of 33.9 cm. At 120 kHz the mean target strength was –47.9 dB (confidence interval of –48.8 to –46.4 dB). This work makes two significant advances: in situ TS measurements have been made that can be confidently attributed to orange roughy, and using a trawl to herd orange roughy past the system resolved the previously intractable problem of fish avoidance.
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  • 39
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-09-29
    Beschreibung: Single-molecule imaging is a powerful technique to visualize molecular interactions and movements. Translation is one of the most interesting targets for researchers with the molecular-imaging skills, since mRNA, tRNA and translation factors interact with or move inside or on the ribosome in an ordered manner. Trans -translation is a bacterial quality control system to rescue the ribosomes stalled at the 3' end of the mRNA, and this phenomenon is recapitulated in vitro with defined factors including two trans -translation-specific entities tmRNA and SmpB. Zhou et al. (Single molecule imaging of the trans -translation entry process via anchoring of the tagged ribosome. J Biochem 2011;149:609-618.) successfully visualized the interaction of the tmRNA–SmpB complex with the ribosome by immobilizing the ribosome on the quartz surface with the HaloTag technology. This ribosome-anchoring system may be useful for the imaging analysis of other processes of translation.
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    Thema: Biologie , Chemie und Pharmazie
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  • 40
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-09-29
    Beschreibung: The lipid mediator sphingosine-1-phosphate (S1P) is generated within cells from sphingosine by two sphingosine kinases (SPHK1 and SPHK2). Intracellularly synthesized S1P is released into the extracellular fluid by S1P transporters, including SPNS2. Released S1P binds specifically to the G protein-coupled S1P receptors (S1PR1/S1P 1 –S1PR5/S1P 5 ), which activate a diverse range of downstream signalling pathways. Recent studies have proposed that one of the central physiological functions of intercellular S1P signalling is in lymphocyte trafficking in vivo because genetic disruption of SPHK1/2, SPNS2 or S1PR1/S1P 1 in mice induces a lymphopenia phenotype. In this review, we discuss the current understanding of intercellular S1P signalling in the context of immunity.
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  • 41
    Publikationsdatum: 2012-09-29
    Beschreibung: Cdc6 is the AAA+ ATPase that assembles prereplicative complexes on replication origins in eukaryotic chromosomes. Recently, the same Cdc6 protein was found to exert two more functions in mammalian cells to promote cell proliferation and survival: ATP-dependent activation of p21 CIP1 - or p27 KIP1 -bound Cdk2-cyclin A/E complexes and obstruction of apoptosome assembly and consequent cell death by forming stable complexes with activated Apaf-1 molecules. These findings not only redefined the biological role of mammalian Cdc6 but also led the discovery of entirely new mechanisms controlling Cdk2 activity and apoptosis. This review focuses on this multi-functional AAA+ ATPase and the newly discovered mechanisms by which it controls the G 1 –S transition and cell survival during proliferation.
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  • 42
    Publikationsdatum: 2012-09-29
    Beschreibung: The pyrimidine reductive catabolic pathway is important for the utilization of uracil and thymine as sources of nitrogen and carbon. The pathway is controlled by three enzymes: dihydropyrimidine dehydrogenase (DPD), dihydropyrimidinase and β-alanine synthase. The putative DPD genes, pydX and pydA , are tandemly arranged in the Pseudomonas putida genome. Intriguingly, a putative transcriptional regulator, PydR, homologous to Escherichia coli RutR, a repressor of the Rut-dependent pyrimidine degradation pathway, is located downstream of pydX and pydA . In this study, we show that a pydA strain of P. putida fails to grow on a minimal media containing uracil or thymine as a sole nitrogen source, demonstrating the physiological importance of DPD in the reductive pathway. The expression of pydA and DPD activity in the absence of uracil were significantly higher in a pydR strain than in the wild-type strain, indicating that PydR acts as a repressor of the pyrimidine reductive pathway in P. putida . Phylogenetic analysis of RutR and PydR suggests that these homologous repressors may have evolved from a common ancestral protein that regulates pyrimidine degradation.
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  • 43
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-09-29
    Beschreibung: Acute inflammation is an indispensable host response to foreign challenges or tissue injury. In healthy conditions, inflammatory processes are self-limiting and self-resolving, suggesting the existence of endogenous mechanisms for the control of inflammation and resolution. A comprehensive understanding of the cellular and molecular events of a well-orchestrated inflammatory response is required, and recent studies have uncovered the roles of endogenous lipid mediators derived from polyunsaturated fatty acids (i.e. lipoxins, resolvins, protectins) in controlling the resolution of inflammation. This review presents recent advances in understanding the formation and action of these mediators, especially focusing on the LC-MS/MS-based lipidomics approach and the emerging roles of eosinophils and eosinophil-derived lipid mediators in controlling acute inflammation and resolution.
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  • 44
    Publikationsdatum: 2012-09-29
    Beschreibung: The cytokine transforming growth factor-beta (TGF-β) has multiple effects in both physiological and pathological conditions. TGF-β is secreted as part of a tripartite complex from which it must be released in order to bind to its receptor. Sequestration of latent TGF-β in the extracellular matrix (ECM) is crucial for proper mobilization of the latent cytokine and its activation. However, contrary to expectation, loss-of-function mutations in genes encoding certain matrix proteins that bind TGF-β yield elevated, rather than decreased, TGF-β levels, posing a ‘TGF-β paradox.’ In this review, we discuss recent findings concerning the relationship of TGF-β, ECM molecules, and latent TGF-β activation and propose a model to resolve the ‘TGF-β paradox.’
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  • 45
    Publikationsdatum: 2012-09-29
    Beschreibung: Colicin E5 cleaves tRNAs for Tyr, His, Asn and Asp in their anticodons to abolish protein synthesis in Escherichia coli . We previously showed how its C-terminal RNase domain, E5-CRD, recognizes the anticodon bases but the catalytic mechanism remained to be elucidated. Although the reaction products with 5'-OH and 2',3'-cyclic phosphate ends suggested a similar mechanism to those of RNases A and T1, E5-CRD does not have the His residues necessary as a catalyst in usual RNases. To identify residues important for the catalytic reaction, mutants as to all residues within 5 Å from the central phosphorus of the scissile phosphodiester bond were prepared. Evaluation of the killing activities of the mutant colicins and the RNase activities of the mutant E5-CRDs suggested direct involvement of Arg33, Lys25, Gln29 and Lys60 in the reaction. Particularly, Arg33 plays a critical role and Ile94 provides a structural support of Arg33. Crystal structure of the complex of E5-CRD(R33Q)/dGpdUp showed structural and binding functional integrity of this mutant protein, suggesting involvement of Arg33 in the catalytic reaction. The structure of the free E5–CRD, we also determined, showed great flexibility of a flap region, which facilitates the access of Lys60 to the substrate in an induced-fit manner.
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  • 46
    Publikationsdatum: 2012-09-29
    Beschreibung: Sulfatide (HSO 3 -3-galactosylceramide), which enriched in lipid rafts of plasma membranes in various epithelial cell lines, is a critical component of host cells for effective production of influenza A virus. However, the function of sulfatide in other virus infections targeting epithelial cells remains unknown. In this study, the effect of sulfatide on infection of human parainfluenza virus type 3 (hPIV3) was demonstrated by using genetically produced sulfatide-enriched cells and by treatment of hPIV3-infected cells with anti-sulfatide monoclonal antibody (GS-5) as well as by addition of sulfatide to the cells. hPIV3 was found to bind to sulfatide in a virus overlay assay and a solid-phase binding assay. Genetic expression of sulfatide in COS-7 cells defective in sulfatide suppressed initial hPIV3 infection and formation of multinucleate virus-infected cells. Treatment of virus-infected LLC-MK2 cells with GS-5 promoted formation of multinucleate cells. In contrast, exogenous addition of sulfatide to hPIV3-infected COS-7 cells and cells expressing the hPIV3- hemagglutinin-neuraminidase ( HN ) gene and fusion ( F ) gene conspicuously reduced the formation of multinucleate cells. The results suggest that sulfatide negatively regulates the fusion process of hPIV3, possibly through interaction with HN or F glycoprotein on the cell surface.
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  • 47
    Publikationsdatum: 2012-09-29
    Beschreibung: Annexin A3 is a protein belonging to the annexin family, and it is mainly present in cellular membranes as a phospholipid-binding protein that binds via the calcium ion. However, its physiological function remains to be clarified. We examined the expression of annexin A3 in mouse tissues and found for the first time that annexin A3 mRNA and its protein were expressed more strongly in adipose tissues than in other tissues. In adipose tissues, annexin A3-expressing cells were present in the stromal vascular fraction, and precisely identical to Pref-1-positive preadipocytes, Pref-1 being an epidermal growth factor repeat-containing transmembrane protein that inhibits adipogenesis. In 3T3-L1 cells, used as a model of adipogenesis, annexin A3 was down-regulated at an early phase of adipocyte differentiation, and this pattern paralleled that of Pref-1. Suppression of annexin A3 in these cells with siRNA caused elevation of the PPAR2 mRNA level and lipid droplet accumulation. In conclusion, our data suggest that annexin A3 is a negative regulator of adipocyte differentiation.
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  • 48
    Publikationsdatum: 2012-09-29
    Beschreibung: Protein phosphorylation by protein tyrosine (Tyr) kinases plays important roles in a variety of signalling pathways in cell growth, differentiation and oncogenesis in animals. Despite the absence of classical Tyr kinases in plants, a similar ratio of phosphotyrosine residues in phosphorylated proteins was found in Arabidopsis thaliana as in human. However, protein kinases responsible for tyrosine phosphorylation in plants except some dedicated dual-specificity kinases still remain unclear. In this study, we found that PKL01, a nuclear Dbf2-related (Ndr) kinase homologue in Lotus japonicus , was autophosphorylated at a tyrosine residue when it was expressed in Escherichia coli , but kinase-dead mutant of PKL01 was not. Tyrosine phophorylation site in PKL01 was identified as Tyr-56 by LC-MS/MS analysis. Recombinant PKL01, which had been dephosphorylated by an alkaline phosphatase, could be phosphorylated again at the Tyr residue when it was incubated with ATP. Furthermore, other Ndr kinases in plants and PKL01 phosphorylated on Tyr residues in the exogenous substrates such as poly(Glu, Tyr) 4:1 and casein. Therefore, the Ndr kinases in plants, which had been assumed as protein serine (Ser)/threonine (Thr) kinases, turned out to be dual-specificity kinases responsible for phosphorylation of Tyr residues and Ser/Thr residues in plant proteins.
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  • 49
    Publikationsdatum: 2012-09-29
    Beschreibung: Mouse UDP-glucuronosyltransferase 1a6 (Ugt1a6) contains two functional copies of 1a6a and 1a6b that share high sequence homology (98%). Only 10 amino acids located around the substrate recognition region are different out of 531 total residues. Although Ugt1a6 plays important roles in conjugating phenolic compounds, the functional characteristics of these isozymes are unclear. We performed functional analyses of mouse Ugt1a6a and Ugt1a6b using two isomeric polyphenols ( trans - and cis -resveratrol). The cDNAs of mouse Ugt1a6a and Ugt1a6b were cloned and constructed as recombinant proteins using a yeast expression system, and kinetic parameters were evaluated. The wild-type Ugt1a6a and Ugt1a6b proteins catalysed trans - and cis -resveratrol 3- O -glucuronidation. Although the K m value for trans -resveratrol was significantly lower for Ugt1a6a compared with Ugt1a6b, the K m values for cis -resveratrol were comparable for the isozymes. Despite high sequence homology, significant kinetic differences were observed between the isozymes. To identify the critical residues for resveratrol glucuronidation, we constructed 10 variants of Ugt1a6a (T81P, N96R, H98Q, L100V, S104P, N115S, I117L, V118T, V119L and D120E). The I117L variant had Ugt1a6b-like enzymatic properties of K m in trans -resveratrol, and V max and K si in cis -form, suggesting that the residues located at position 117 of Ugt1a6a and Ugt1a6b play an important role in resveratrol glucuronidation.
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  • 50
    Publikationsdatum: 2012-10-13
    Beschreibung: Greenstreet, S. P. R., Rossberg, A. G., Fox, C. J., Le Quesne, W. J. F., Blasdale, T., Boulcott, P., Mitchell, I., Millar, C., and Moffat, C. F. 2012. Demersal fish biodiversity: species-level indicators and trends-based targets for the Marine Strategy Framework Directive. – ICES Journal of Marine Science, 69: 1789–1801. The maintenance of biodiversity is a fundamental theme of the Marine Strategy Framework Directive. Appropriate indicators to monitor change in biodiversity, along with associated targets representing "good environmental status" (GES), are required to be in place by July 2012. A method for selecting species-specific metrics to fulfil various specified indicator roles is proposed for demersal fish communities. Available data frequently do not extend far enough back in time to allow GES to be defined empirically. In such situations, trends-based targets offer a pragmatic solution. A method is proposed for setting indicator-level targets for the number of species-specific metrics required to meet their trends-based metric-level targets. This is based on demonstrating significant departures from the binomial distribution. The procedure is trialled using North Sea demersal fish survey data. Although fisheries management in the North Sea has improved in recent decades, management goals to stop further decline in biodiversity, and to initiate recovery, are yet to be met.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 51
    Publikationsdatum: 2012-10-13
    Beschreibung: Cabanellas-Reboredo, M., Alós, J., Palmer, M., and Morales-Nin, B. 2012. Environmental effects on recreational squid jigging fishery catches. – ICES Journal of Marine Science, 69: 1823–1830. Experimental fishing sessions simulating the operating procedures of the recreational fishery for the European squid that operates at inshore Palma Bay (Balearic Islands, Spain) were conducted to investigate the effects of environmental variables on squid catches. The catch per unit of effort (cpue) of recreational-like jigging sessions showed a seasonal pattern (higher cpue during colder months). Two alternative hypotheses can explain such a pattern. First, squid could migrate inshore during colder months to seek spatio-temporal windows within which the sea temperature maximize spawning success. Second, the timing of the seasonal reproductive peak and the growth rate of any given cohort would result in a higher percentage of squid whose body size is greater than the gear-specific vulnerability threshold during the colder months. The combination of environmental variables that maximized cpue was a low sea surface temperature, a low windspeed, low atmospheric pressure, and days close to the new moon. A specific period of the day, narrowly around sunset, favoured the catches. Within this narrow period, the sunlight is still sufficient to allow the recreational fishing lures to be effective, and the squid have already shifted to a more active pattern of movement characteristic of the night-time period.
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  • 52
    Publikationsdatum: 2012-10-13
    Beschreibung: McCully, S. R., Scott, F., and Ellis, J. R. 2012. Lengths at maturity and conversion factors for skates (Rajidae) around the British Isles, with an analysis of data in the literature. –ICES Journal of Marine Science, 69: 1812–1822. Biological data on skates (Rajidae) from around the British Isles were collected between 1992 and 2010. The relationship between total length and weight for nine species ( Amblyraja radiata , Dipturus batis- complex, Leucoraja fullonica , L. naevus , Raja brachyura , R. clavata , R. microocellata , R. montagui , and R. undulata ) are provided for each sex and ICES ecoregion (when significantly different). Conversion factors for disc width to total length are provided. The lengths at first maturity and of the largest immature skates are reported for each sex, and the lengths at 50% maturity are estimated. Spatial differences in the length at maturity of R. clavata (females only) and L. naevus (both sexes) were observed. The lengths at maturity are discussed in relation to the results of earlier studies, and methodological differences are considered to have influenced reputed decreases in the length at maturity. A more standardized approach to collecting and reporting maturity information is required if potential spatial differences and temporal changes are to be investigated.
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  • 53
    Publikationsdatum: 2012-10-13
    Beschreibung: Parada, J. M., Outeiral, R., Iglesias, E., and Molares, J. 2012. Assessment of goose barnacle ( Pollicipes pollicipes Gmelin, 1789) stocks in management plans: design of a sampling program based on the harvesters' experience. – ICES Journal of Marine Science, 69: 1840–1849. Management plans of coastal marine resources require a wealth of information on socioeconomic topics, harvesting activities, population dynamics, and stock status. Moreover, the information provided by technical experts must take into account the needs of the managers. It must also adapt to schedules to serve a useful purpose. In many cases, the methodologies used by research teams are not directly applicable as they may be too complicated, aimed at specific objectives related to basic scientific work, or too costly to apply to long-term monitoring of extensive shellfish beds. Also, rocky coastlines exposed to heavy wave action preclude the use of sampling techniques that involve time-consuming data collection. This paper proposes a quick and simple methodology for gathering data in the field, based on the knowledge of the harvesters, to obtain stock assessments in keeping with their information needs. This methodology uses coverage percentage as an abundance index and weighting factor for the biometric information gathered from 50 specimens in each sampling. The sampling design uses the knowledge of the harvesters to define homogeneous strata. The results are in agreement with both the scientific-technical knowledge and the harvesters' knowledge of the populations being analysed.
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  • 54
    Publikationsdatum: 2012-10-13
    Beschreibung: Rougier, T., Lambert, P., Drouineau, H., Girardin, M., Castelnaud, G., Carry, L., Aprahamian, M., Rivot, E., and Rochard, E. 2012. Collapse of allis shad, Alosa alosa , in the Gironde system (southwest France): environmental change, fishing mortality, or Allee effect? – ICES Journal of Marine Science, 69: 1802–1811. At the end of the 20th century the allis shad population in the Gironde was the largest in Europe. During the first decade of the 21st century, catches declined dramatically by two orders of magnitude, and a fishery moratorium was implemented in 2008. This deterioration in the status of the stock was confirmed by three independent assessments (abundance of juveniles and of potential and effective spawners). Three hypotheses on the cause of the collapse were examined: (i) an environmental change in freshwater and/or in the estuary; (ii) an increase in marine and/or estuarine mortality; and (iii) the presence of an Allee effect. Changes in flow, temperature, and water quality over the period were inconclusive, but remain a possible causative factor. The instantaneous rate of marine (mean: 0.7 year –1 , s.e. 0.1 year –1 ) and estuarine (mean: 2.6 year –1 , s.e. 0.1 year –1 ) mortalities showed no trend between 1991 and 2003. Nevertheless, the past high estuarine (fishing) mortalities combined with a demographic Allee effect in the reproduction dynamics could explain the population collapse and hamper the stock recovery under the moratorium. This conclusion is, however, tentative as it was not possible to prove the presence of the density-dependent mechanism associated with the demographic Allee effect.
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  • 55
    Publikationsdatum: 2012-10-13
    Beschreibung: Krumsick, K. J., and Rose, G. A. 2012. Atlantic cod ( Gadus morhua ) feed during spawning off Newfoundland and Labrador. – ICES Journal of Marine Science, 69: 1701–1709. We test a current assumption that Atlantic cod ( Gadus morhua ) off Newfoundland and Labrador, Canada, do not feed during the protracted spawning season (March–September). Stomach contents were analysed from 10 473 cod from four Northwest Atlantic Fisheries Organization regions (2J, 3K, 3L, and 3Ps) over 9 years from which gonads were also analysed to determine sex and maturity status. Adult cod in spawning condition did feed in all regions, usually at rates equivalent to or even greater than non-spawning fish and juveniles. Both sexes fed during spawning, though females consumed lesser amounts. Regional differences were evident. The total fullness index was greater in the northern (2J) than the southern (3Ps) region, with no consistent differences between spawners and non-spawners. The most southerly region (3Ps) exhibited the greatest prey diversity, the northern region (2J) the least. Shrimp was the major diet item in the northern regions. Capelin, zooplankton, crab, and other fish increased in importance to the south. Differences in prey items between non-spawning and spawning individuals of both sexes were possibly related to spawning behaviour. Models using consumption rates should not assume that cod do not feed during the protracted spawning season in these waters.
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  • 56
    Publikationsdatum: 2012-10-13
    Beschreibung: Methratta, E.T., and Link, J.S. 2012. Feeding hotspots for four northwest Atlantic groundfish species. — ICES Journal of Marine Science, 69: 1710–1721. We used predator distribution and stomach content data to estimate the annual per capita rate of consumption for four representative predator species from the northwestern Atlantic Ocean, and considered how consumption is influenced by depth, bottom salinity, sediment grain size, location variables, and species-specific diet components. We found that geographic variables and species-specific prey resources were significantly associated with consumption rates, a pattern consistent with predator-prey theory. Prey categories comprised of fish were particularly important for a more mobile predator (silver hake Merluccius bilinearis) , whereas benthic invertebrate prey were consistently important for a more sedentary predator (little skate Raja erinacea ). Hotspots in consumption rates that overlap with particular prey resources were highlighted by the significance of location variables (longitude) for winter flounder Pseudopleuronectes americanus , silver hake, little skate, and sea raven Hemitripterus americanu s. Depth was an important explanatory factor for consumption by little skate, but the explanatory value of abiotic habitat factors was low for the other three species. Greater emphasis on species-specific food habits, migratory patterns, and ecological interactions at the synoptic scales relevant to fisheries is needed for fisheries management.
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  • 57
    Publikationsdatum: 2012-10-13
    Beschreibung: Hare, J.A., Manderson, J.P., Nye, J.A., Alexander, M.A., Auster, P.J., Borggaard, D.L., Capotondi, A.M., Damon-Randall, K.B., Heupel, E., Mateo, I., O'Brien, L., Richardson, D.E., Stock, C.A., and Biege, S.T. 2012. Cusk ( Brosme brosme ) and climate change: assessing the threat to a candidate marine fish species under the US Endangered Species Act. – ICES Journal of Marine Science, 69: 1753–1768. In the Northwest Atlantic Ocean cusk ( Brosme brosme ) has declined dramatically, primarily as a result of fishing activities. These declines have led to concern about its status, which has prompted reviews under the US Endangered Species Act and the Canadian Species at Risk Act. Changes in distribution and abundance of a number of marine fish in the Northwest Atlantic have been linked to climate variability and change, suggesting that both fishing and climate may affect the status of cusk. Our goal was to evaluate potential effects of climate change on Northwest Atlantic cusk distribution. Coupling a species niche model with the output from an ensemble of climate models, we projected cusk distribution in the future. Our results indicate cusk habitat in the region will shrink and fragment, which is a result of a spatial mismatch between high complexity seafloor habitat and suitable temperature. The importance of habitat patch connectivity for cusk is poorly understood, so the population-level consequences of climate-related habitat fragmentation are uncertain. More broadly, climate change may reduce appropriate thermal habitat and increase habitat fragmentation for other cold-water species in the region; thereby, increasing the potential for regional overexploitation and extirpation.
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  • 58
    Publikationsdatum: 2012-10-13
    Beschreibung: Otterå*, H., Agnalt, A-L., Thorsen, A., Kjesbu, O.S., Dahle, G., and Jørstad, K. 2012. Is spawning time of marine fish imprinted in the genes? A two-generation experiment on local Atlantic cod ( Gadus morhua L.) populations from different geographical regions. – ICES Journal of Marine Science, 69: 1722–1728. Spawning time (onset of spawning) in Atlantic cod ( Gadus morhua L.) was monitored in an experimental setup and combined with modelled spawning time estimates from the wild. The experiment broodstock were collected from several geographical areas and kept in a common environment. Their spawning times in 2004 were compared with the spawning times of their daughters in 2009 and 2010. Daughter spawning time was highly correlated with that of the mother, indicating genetic regulation of spawning time. However, large individual variation in spawning time was observed. The modelling data suggests a north-south gradient in onset of spawning along the Norwegian coast, driven by differences in temperature, i.e. later dates of spawning in the north.
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  • 59
    Publikationsdatum: 2012-10-13
    Beschreibung: Hinrichsen, H-H., Hüssy, K., and Huwer, B. 2012. Spatio-temporal variability in western Baltic cod early life stage survival mediated by egg buoyancy, hydrography and hydrodynamics. – ICES Journal of Marine Science, 69: 1744–1752. To disentangle the effects of different drivers on recruitment variability of marine fish, a spatially and temporally explicit understanding of both the spawning stock size and the early life stage dynamics is required. The objectives of this study are to assess the transport of western Baltic cod early life stages as well as the variability in environmentally-mediated survival along drift routes in relation to both spatial (within and between different spawning areas) and temporal (interannual and seasonal) dynamics. A spatially and temporally highly-resolved biophysical model of the Baltic Sea was used to describe mortalities and survival success of eggs and yolk-sac larvae—represented by individual, virtual drifters—as predicted proportions of drifters that either died due to bottom contact or lethal temperatures, or that survived up to the end of the yolk-sac larval stage. The environmental conditions allowing survival of cod and yolk-sac larvae indicate that favourable conditions predominately occurred during the late spawning season, while minimum survival rates could be expected from January to March. The spatial analysis of different spawning areas revealed highest survival chances in the Kattegat, intermediate survival in the Great Belt, and only low survival in the Sound, Kiel Bay and Mecklenburg Bay.
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  • 60
    Publikationsdatum: 2012-10-13
    Beschreibung: Ovegård, M., Berndt, K., and Lunneryd, S-G. 2012. Condition indices of Atlantic cod ( Gadus morhua ) biased by capturing method. – ICES Journal of Marine Science, 69: 1781–1788. In studies evaluating the condition and general health status of fish stocks, the method used for catching the fish is seldom considered as a factor of importance. In this study, condition indices were compared between cod caught in pots, gillnets, and on hooks in the same geographical area. The results showed that cod ( Gadus morhua ) caught on baited gear types (pots and hooks) generally displayed a lower condition and an older age (i.e. suggesting a lower growth rate) compared to cod caught in gillnets. It is unclear whether these results merely illustrate divergent behavioural responses in fish originating from one single population, or if these divergent behavioural components represent distinct subpopulations displaying different mean conditions and growth rates. Regardless of the underlying causes, the results not only show that parts of the Baltic cod stock are in extremely poor condition, they also indicate that different gear types used in the same area could target similar-sized conspecifics exhibiting large differences in condition and size-at-age. The potential impact of the difference in condition between the pots and other gear types could hamper the implementation of the cod pot as a potentially seal-safe and sustainable fishing method.
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  • 61
    Publikationsdatum: 2012-10-13
    Beschreibung: Hüssy, K., Coad, J. O., Farrell, E. D., Clausen, L. W., and Clarke, M. W. 2012. Sexual dimorphism in size, age, maturation, and growth characteristics of boarfish ( Capros aper ) in the Northeast Atlantic – ICES Journal of Marine Science, 69: 1729–1735. Boarfish ( Capros aper ) have, in recent years, become of increasing commercial importance due to their apparent increase in abundance in the Northeast Atlantic. This study presents detailed biological information relevant to understanding stock structure and dynamics. Boarfish are a long-lived species that reach a maximum age of 〉30 years. The size distribution is skewed towards larger sizes, as expected from an unexploited stock with sexual dimorphism, where females are, on average, larger than males. No seasonal effects occur in size distribution and sex ratio, indicating that females and males stay together in shoals throughout the year. Females become increasingly dominant in abundance at larger sizes and older ages. There is no significant difference in length at maturity nor age at maturity between the two sexes, with L 50 = 9.7 cm and A 50 = 3.4 years. Growth is dimorphic following von Bertalanffy growth characteristics, with a common t 0 = –2.897, but significantly different K and L inf values, where females have K = 0.145 and L inf = 16.519 and males have K = 0.181 and L inf = 14.437. The sexual dimorphism in size is thus caused by sex-specific growth and longevity patterns.
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  • 62
    Publikationsdatum: 2012-10-13
    Beschreibung: Strehlow, H. V., Schultz, N., Zimmermann, C., and Hammer, C. 2012. Cod catches taken by the German recreational fishery in the western Baltic Sea, 2005–2010: implications for stock assessment and management. – ICES Journal of Marine Science, 69: 1769–1780. Next to the commercial fishery, the recreational fishery plays an important role in the removal of biomass from fish stocks. In this study, we present estimates of German recreational cod ( Gadus morhua ) catches in the western Baltic Sea between 2005 and 2010. Fishing effort was estimated using a stratified mail survey and annual sales of fishing licences. Catch per unit effort was estimated by stratified random sampling of access points and interviews about completed trips. Length distributions of cod catches were acquired by sampling recreational cod catches from charter boats and data from community fishing events. Estimates of the total cod biomass removed by the recreational fishery fluctuated between 2159 t in 2009 and 4127 t in 2005. Annual recreational fishery cod harvests accounted for a significant share of the total landings, with a yearly variation from 34 to 70% of the German commercial cod landings from the western Baltic Sea. The majority of recreational fishery cod catches were taken from private boats and charter vessels. Because of the amount and specifically the variability of the recreational catches, they are important for the assessment and management of the resource and, therefore, need to be surveyed annually.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 63
    Publikationsdatum: 2012-10-13
    Beschreibung: Xu, C., and Schneider, D. C. 2012. Efficacy of conservation measures for the American lobster: reproductive value as a criterion – ICES Journal of Marine Science, 69: 1831–1839. Reproductive value takes into account both current and future value, effectively characterizing the value of an individual to the population. In this study, we use reproductive value as a criterion to evaluate the effectiveness of several conservation measures that have been undertaken or proposed for the American lobster ( Homarus americanus ) fishery in Newfoundland. These measures are slot fishing (i.e. no fishing below or above certain sizes), marine protected areas (MPAs), V-notching, and window fishing (i.e. no fishing within a size window). Reproductive value was estimated by using catch length data available from commercial fisheries and research surveys. We found that MPAs had the greatest effect, followed by V-notching and slot-fishing. MPAs resulted in an average increase of 64.9% in total reproductive value, V-notching an average increase of 18.3%, and slot fishing an average increase of 16.8%. In general, window fishing was not effective unless the size of the window was large. For instance, an average increase of 8.7% in total reproductive value would require a wide window (100–129 mm). Our results provide a scientific basis for evaluating conservation measures in a way that integrates population demographical information with information from commercial fisheries and research surveys.
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  • 64
    Publikationsdatum: 2012-10-13
    Beschreibung: Hüssy, K., Hinrichsen, H.-H., and Huwer, B. 2012. Hydrographic influence on the spawning habitat suitability of western Baltic cod ( Gadus morhua ) – ICES Journal of Marine Science, 69: 1736–1743. Recruitment variability of marine fish is influenced by the reproductive potential of the stock (i.e. stock characteristics and abundance) and the survival of early life stages, mediated by environmental conditions of both a physical (water temperature, salinity and oxygen conditions, ocean currents) and a biological nature (i.e. food, predators). The objective of this study is to assess the importance of variability in environmental conditions within different western Baltic cod spawning grounds for egg survival. Habitat identification was based on environmental threshold levels for egg survival and development and ambient hydrographical conditions at different times during the spawning season. The long-term resolution of environmental conditions allowing survival of western Baltic cod eggs indicates that favourable conditions predominantly occurred during the late spawning season in April/May, while minimum survival rates could be expected from January to March. Unsuitable time periods and habitats exhibiting the highest mortality rates are exclusively characterized by ambient water temperatures below the critical survival threshold. Despite the strong influence of water temperature on habitat suitability, the impact of habitat suitability on recruitment was not clearly defined, suggesting that other mechanisms regulate year class strength.
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  • 65
    facet.materialart.
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    Oxford University Press
    Publikationsdatum: 2012-10-13
    Beschreibung: Piet, G. J., and Hintzen, N. T. 2012. Indicators of fishing pressure and seafloor integrity. – ICES Journal of Marine Science, 69: 1850–1858. This study calculates a suite of indicators reflecting the spatial extent of fishing and its impact on the seafloor and discusses the usefulness of these indicators to inform future management and the issues to consider. It explores several methods to calculate the indicators and shows that they can be informative to report on both fishing pressure and the status of the seafloor. However, although observed overall trends were robust against the specific method of calculation, the absolute values vary greatly with the calculation method. As both aspects are important from a policy perspective, agreement on the methodology to calculate the indicators is required. This study based on the Dutch Exclusive Economic Zone (EEZ) shows that it is possible to calculate indicators required to inform decision-makers on the pressure of fishing as well as the status of the seafloor pending a decision on the following issues: (i) choice of an appropriate grid cell resolution, (ii) use of interpolated VMS tracks instead of VMS position registrations, (iii) choice of an "intensity threshold" dependent on the benthic community recovery capacity, and (iv) the level of confidence required when assessing if an area is not impacted.
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  • 66
    Publikationsdatum: 2012-08-22
    Beschreibung: Jennings, S., and Le Quesne, W. J. F. 2012. Integration of environmental and fishery management in Europe. – ICES Journal of Marine Science, 69: . Policy drivers for integrating environmental and fishery management in Europe have never been so strong. Scientists calling for better integration now have the opportunity to help deliver it. The main challenge is providing relevant evidence on short time-scales using existing knowledge. Policies, scientists, and society largely agree that management targets should be linked to achieving sustainability, but research often fails to show when fishing impacts ‘matter’ in relation to sustainability criteria. If targets for ecosystem functions or processes are to complement more tractable targets for species and habitats, scientists will need to show why impacts ‘matter’ and when they become unsustainable. For now, and to meet ambitious and pressing policy timetables, priority should be given to developing credible targets for impacts with a high risk of compromising sustainability, rather than dissipating research and advisory effort to achieve broader coverage of state, function, and process. Impacts on sensitive species and habitats often compromise sustainability; thus, setting targets for them is a priority. Meeting these targets will often require management measures that are expected to diminish risks of other unsustainable impacts. Fast and significant progress towards integration could be achieved by incorporating measures to meet environmental targets for sensitive species and habitats into fishery management plans.
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  • 67
    Publikationsdatum: 2012-08-22
    Beschreibung: MacNamara, R., and McCarthy, T. K. 2012. Size-related variation in fecundity of European eel ( Anguilla anguilla) . – ICES Journal of Marine Science, 69: . Declining European eel ( Anguilla anguilla ) recruitment has focused attention on conservation of potential spawners leaving continental waters. Fecundity of wild, seaward-migrating silver-phase eels was shown to be size-related and higher than previously reported from artificial maturation experiments. Reliable information on fecundity is essential for stock modelling and future development of eel management policies.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 68
    Publikationsdatum: 2012-08-22
    Beschreibung: López-López, L., Preciado, I., Villamor, B., Velasco, F., Iglesias, M., Nogueira, E. Gutierrez-Zabala, J. L., and Olaso, I. 2012. Is juvenile anchovy a feeding resource for the demersal community in the Bay of Biscay? On the availability of pelagic prey to demersal predators. – ICES Journal of Marine Science, 69: . The role that juvenile anchovy ( Engraulis encrasicolus ) play as a food resource for the demersal community in the southern Bay of Biscay is assessed using 21 years of anchovy abundance data and demersal predator diets. During the study period, a total of 26 fish and elasmobranch species preyed on anchovy either frequently or occasionally. Predators with a crustacean-based diet targeted the smaller anchovy individuals. The size range of anchovy juveniles (centred at 7.5–8.9 cm) was comparable to that of the largest nektonic–benthic crustaceans, but generally smaller than other demersal and pelagic fish prey. Hake ( Merluccius merluccius ) and megrim ( Lepidorhombus whiffiagonis ) were the predators that consumed the highest number of anchovy, one of the main prey items driving the variability of their diets. Anchovy consumption conformed only partially to the abundance of anchovy in the southern Bay of Biscay, suggesting that factors other than abundance might condition its availability to demersal predators. Prey size could be one of them, as the size of the anchovy preyed on proved to be significantly smaller than the individuals collected with bottom trawls. However, other factors, such as the vertical position of the shoals of anchovy juveniles, could also constrain anchovy availability to demersal predators.
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  • 69
    Publikationsdatum: 2012-08-22
    Beschreibung: Le Port, A., Lavery, S., and Montgomery, J. C. 2012. Conservation of coastal stingrays: seasonal abundance and population structure of the short-tailed stingray Dasyatis brevicaudata at a Marine Protected Area. – ICES Journal of Marine Science, 69: . Elasmobranch (shark, ray, and skate) populations around the world are in decline, and effective conservation measures are urgently needed. Marine Protected Areas (MPA) placed in locations important for key life-history stages may form part of an effective conservation strategy. In this context, we examined the seasonal abundance and population structure of the short-tailed stingray ( Dasyatis brevicaudata ) at an offshore MPA in northeastern New Zealand, and the reported use of this location as a mating ground. Diver surveys were conducted from 2004 to 2007 at the Poor Knights Islands Marine Reserve (PKIMR). During this time, we observed: (i) a substantial increase in adult and subadult numbers, particularly females during the suggested breeding season, and a corresponding increase in females bearing fresh mating scars; and (ii) large numbers of smaller (probably immature) D. brevicaudata individuals of both sexes from spring to autumn. These results suggest that the PKIMR acts as both a mating aggregation location and a nursery for this species. We suggest that for coastal stingrays such as D. brevicaudata , small MPAs may be effective at protecting key life-history stages, but that as movements outside of reserve boundaries also occur, additional management tools may also be necessary.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 70
    Publikationsdatum: 2012-08-22
    Beschreibung: Butcher, P. A., Leland, J. C., Broadhurst, M. K., Paterson, B. D., and Mayer, D. G. 2012. Giant mud crab ( Scylla serrata ): relative efficiencies of common baited traps and impacts to discards. – ICES Journal of Marine Science, 69: . This study was initiated in response to a scarcity of data on the efficiency, selectivity and discard mortality of baited traps to target Scylla serrata . Five replicates of four traps, including "hoop nets", rigid "wire pots", and collapsible "round" and "rectangular" pots were deployed for 3, 6 and 24 h in two Australian estuaries. Trapped S. serrata were "discarded" into cages and monitored with controls over 3 d. All S. serrata were assessed for damage, while subsets of immediately caught and monitored individuals had haemolymph constituents quantified as stress indices. All traps retained similar-sized (8.1–19.1 cm carapace width) S. serrata , with catches positively correlated to deployment duration. Round pots were the most efficient for S. serrata and fish—mostly Acanthopagrus australis (3% mortality). Hoop nets were the least efficient and were often damaged. No S. serrata died, but 18% were wounded (biased towards hoop nets), typically involving a missing swimmeret. Physiological responses were mild and mostly affected by biological factors. The results validate discarding unwanted S. serrata for controlling exploitation, but larger mesh sizes or escape vents in pots and restrictions on hoop nets would minimise unnecessary catches, pollution and ghost fishing.
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  • 71
    Publikationsdatum: 2012-08-22
    Beschreibung: Papaioannou, E. A., Vafeidis, A. T., Quaas, M. F., and Schmidt, J. O. 2012. The development and use of a spatial database for the determination and characterization of the state of the German Baltic small-scale fishery sector. – ICES Journal of Marine Science, 69: . Although substantial progress has been made in the acquisition and analysis of fishery data, the small-scale fishery (SSF) sector is frequently data deficient, with relevant primary data often being fragmented and incomplete. Also, in contrast to the case of the larger scale sector, a coherent methodological framework for the assessment of the SSF has, in most cases, not been formulated. In the present study, the methodology of developing a database for the German Baltic SSF sector is presented. The aim of the database is to combine fishery primary data effectively and enable the sound determination and characterization of the German Baltic SSF sector. Data used include, among others, fleet data derived from the European Community Fleet Register (CFR) database and logbook data from the German Federal Office for Agriculture and Food (BLE). The database includes information on the technical specifications of SSF vessels (length, engine power, etc.); the sector's operational range; main target species; fishing grounds; landing ports; and weight and price of landings. Results of employing the database for profiling the state of the SSF sector (in 2008) are presented. The results demonstrate the benefits of such an approach within the framework of managing coastal fish resources and fishing activities.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 72
    Publikationsdatum: 2012-04-10
    Beschreibung: Da Rocha, J-M., Gutiérrez, M-J., and Cerviño, S. 2012. Reference points based on dynamic optimization: a versatile algorithm for mixed-fishery management with bioeconomic age-structured models. – ICES Journal of Marine Science, 69: 660–669. Single-species management objectives may not be consistent within mixed fisheries. They may lead species to unsafe situations, promote discarding of over-quota, and/or misreporting of catches. We provide an algorithm for characterizing bioeconomic reference points for a mixed fishery as the steady-state solution of a dynamic optimal management problem. The optimization problem takes into account that: (i) species are caught simultaneously in unselective fishing operations, and (ii) intertemporal discounting and fleet costs relate to reference points to discounted economic profits along optimal trajectories. We illustrate how the algorithm can be implemented by applying it to the European northern hake stock ( Merluccius merluccius ), where fleets also capture northern megrim ( Lepidorhombus whiffiagonis ) and northern anglerfish ( Lophius piscatorius and Lophius budegassa ). We find that optimal mixed management leads to a target reference point that is quite similar to two-thirds of the F msy single-species (hake) target. Mixed management is superior to single-species management because it leads the fishery to higher discounted profits, with higher long-term spawning-stock biomass for all species. We calculate that the losses due to the use of the F msy single-species (hake) target in this mixed fishery account for 11.4% of total discounted profits.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 73
    Publikationsdatum: 2012-04-10
    Beschreibung: Probst, W. N., Stelzenmüller, V., and Fock, H. 2012. Using cross-correlations to assess the relationship between time-lagged pressure and state indicators: an exemplary analysis of North Sea fish population indicators. – ICES Journal of Marine Science, 69: 670–681. A sustainable ecosystem-based management, as postulated by the European Union-Marine Strategy Framework Directive (MSFD), requires a sound understanding of the cause–effect relationships between human pressures and ecosystem states. In this study, cross-correlations are used to introduce a protocol for the analysis of time-lagged relationships between pressure and state indicators. To perform meaningful cross-correlations, the time-series of the pressure and the state indicator should be prewhitened by fitting autoregressive integrated moving average models to the pressure indicator time-series. This study provides some theoretical examples on the implications of non-prewhitened and prewhitened cross-correlations and exemplifies the use of prewhitened cross-correlations to compare the pressure–state relationship of a well-established indicator suite vs. the relationship of a new indicator proposed under the MSFD. The established indicator suite is fishing mortality ( F ) vs. spawning-stock biomass ( SSB ), the new indicator suite is F vs. the 95% percentile of the length frequency distribution ( L 95 ). The L 95 aims to characterize the proportion of large individuals within a population. The prewhitened cross-correlations for F and SSB are significant for all four analysed species (cod, haddock, saithe, and plaice), the L 95 is correlated with F for cod, haddock, and saithe. However, the L 95 was also influenced by the annual survey catch and recruitment.
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  • 74
    Publikationsdatum: 2012-04-10
    Beschreibung: Kraak, S. B. M., Reid, D. G., Gerritsen, H. D., Kelly, C. J., Fitzpatrick, M., Codling, E. A., and Rogan, E. 2012. 21st century fisheries management: a spatio-temporally explicit tariff-based approach combining multiple drivers and incentivising responsible fishing. – ICES Journal of Marine Science, 69: 590–601. Traditionally fisheries management has focused on biomass and mortality, expressed annually and across large management units. However, because fish abundance varies at much smaller spatio-temporal scales, fishing mortality can potentially be controlled more effectively if managed at finer scale. The ecosystem approach requires more indicators at finer scales as well. Incorporating ecosystem targets would need additional management tools with potentially conflicting results. We present a simple, integrated, management approach that provides incentives for "good behaviour". Fishers would be given a number of fishing-impact credits, called real-time incentives (RTIs), to spend according to spatio-temporally varying tariffs per fishing day. RTI quotas and tariffs could be based on commercial stocks and ecosystem targets. Fishers could choose how to spend their RTIs, e.g. by limited fishing in high-catch or sensitive areas or by fishing longer in lower-catch or less sensitive areas. The RTI system does not prescribe and forbid, but instead allows fishers to fish wherever and whenever they want; ecosystem costs are internalized and fishers have to take them into account in their business decisions. We envisage no need for traditional landings or catch quotas for the fleets while operating under the scheme. The approach could facilitate further devolution of responsibility to industry.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 75
    Publikationsdatum: 2012-04-10
    Beschreibung: Thorson, J. T., Stewart, I. J., and Punt, A. E. 2012. Development and application of an agent-based model to evaluate methods for estimating relative abundance indices for shoaling fish such as Pacific rockfish ( Sebastes spp.). – ICES Journal of Marine Science, 69: 635–647. Many marine fish, including Pacific rockfish ( Sebastes spp.), exhibit habitat-selective and shoaling behaviours, which can lead to imprecision when using survey data to estimate an annual index of stock abundance. We develop a spatial agent-based model (ABM) for Pacific rockfish, which generates data similar to those observed in existing bottom-trawl surveys and can represent various spatial and shoaling behaviours. We use the ABM to evaluate the performance of a model that uses mixture distribution methods to account for fish shoals and delta-methods to account for range expansion or contraction. This delta-mixture model is compared with conventional delta-generalized linear models (delta-GLMs) and a quantile regression delta-model. The delta-mixture increases precision by 15% relative to delta-GLMs in estimated abundance indices when shoaling behaviours are present, whereas precision is similar between delta-GLM and delta-mixture models when shoals are absent. The delta-quantile method has similar improvements over conventional delta-GLM methods, and the improved precision from delta-mixture and delta-quantile methods is decreased but not eliminated by decreased sampling intensities. These simulations represent the first evaluation of delta-mixture models for index standardization and show a substantial improvement over conventional delta-GLMs for shoaling species such as Pacific rockfish.
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 76
    Publikationsdatum: 2012-04-10
    Beschreibung: O'Driscoll, R. L., de Joux, P., Nelson, R., Macaulay, G. J., Dunford, A. J., Marriott, P. M., Stewart, C., and Miller, B. S. 2012. Species identification in seamount fish aggregations using moored underwater video. – ICES Journal of Marine Science, 69: 648–659. Acoustic surveys of New Zealand deep-water seamounts often show fish aggregations up to 150 m high on the summit. Although bottom trawls on the seamount slopes catch predominantly orange roughy ( Hoplostethus atlanticus ), species composition in the midwater plumes is extremely uncertain. In June 2010, moored underwater video cameras were deployed on the summit of the Morgue seamount (summit depth 890 m), a feature that has been closed to fishing since 2001. Cameras and lights were timed to come on for 2 min every 2 h. Fish response to the mooring was monitored using vessel-mounted echosounders. Moored cameras confirmed that orange roughy were present up to 70 m above the seamount summit. Orange roughy made up 97% of the fish identified from the video. Other species observed included smooth oreo ( Pseudocyttus maculatus ), spiky oreo ( Neocyttus rhomboidalis ), deep-water dogfish, cardinalfish ( Epigonus spp.), and squid. Total along-track backscatter from the plume varied by a factor of 25 over a period of hours. Peak acoustic densities in the plume (equivalent to 20 orange roughy m –3 ) were an order of magnitude higher than peak visual estimates (0.64 orange roughy m –3 ), but relative densities between paired video and acoustic observations were generally consistent.
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  • 77
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-04-10
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    Thema: Biologie , Geologie und Paläontologie , Physik
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  • 78
    Publikationsdatum: 2012-04-10
    Beschreibung: Lambert, G. I., Hiddink, J. G., Hintzen, N. T., Hinz, H., Kaiser, M. J., Murray, L. G., and Jennings, S. 2012. Implications of using alternative methods of vessel monitoring system (VMS) data analysis to describe fishing activities and impacts. – ICES Journal of Marine Science, 69: 682–693. Understanding the spatial distribution and intensity of fishing activity is a prerequisite for estimating fishing impacts on seabed biota and habitats. Vessel monitoring system data provide information on fishing activity at large spatial scales. However, successive position records can be too infrequent to describe the complex movements fishing vessels make. High-frequency position data were collected to evaluate how polling frequency and the method of analysis influenced the estimates of fishing impact on the seabed and associated epifaunal communities. Comparisons of known positions with predictions from track interpolation revealed that the performance of interpolation depended on fleet behaviour. Descriptions and indicators of fishing intensity were influenced significantly by the analytical methods (track reconstruction, density of position records) and grid-cell resolution used for the analysis. These factors can lead to an underestimation of fishing impact on epifaunal communities. It is necessary to correct for such errors to quantify the effects of fishing on various ecosystem components and hence to inform ecosystem-based management. Polling at intervals of 30 min would provide a desirable compromise between achieving precise estimates of fishing impacts on the seabed and minimizing the cost of data collection and handling.
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  • 79
    Publikationsdatum: 2012-08-29
    Beschreibung: Feed additives such as ractopamine and salbutamol are pharmacologically active compounds, acting primarily as β-adrenergic agonists. This study was designed to investigate whether the sulfation of ractopamine and salbutamol may occur under the metabolic conditions and to identify the human cytosolic sulfotransferases (SULTs) that are capable of sulfating two major feed additive compounds, ractopamine and salbutamol. A metabolic labelling study showed the generation and release of [ 35 S]sulfated ractopamine and salbutamol by HepG2 human hepatoma cells labelled with [ 35 S]sulfate in the presence of these two compounds. A systematic analysis using 11 purified human SULTs revealed SULT1A3 as the major SULT responsible for the sulfation of ractopamine and salbutamol. The pH dependence and kinetic parameters were analyzed. Moreover, the inhibitory effects of ractopamine and salbutamol on SULT1A3-mediated dopamine sulfation were investigated. Cytosol or S9 fractions of human lung, liver, kidney and small intestine were examined to verify the presence of ractopamine-/salbutamol-sulfating activity in vivo . Of the four human organs, the small intestine displayed the highest activity towards both compounds. Collectively, these results imply that the sulfation mediated by SULT1A3 may play an important role in the metabolism and detoxification of ractopamine and salbutamol.
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    Thema: Biologie , Chemie und Pharmazie
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  • 80
    Publikationsdatum: 2012-08-29
    Beschreibung: Information on the structural scaffold for tau aggregation is important in developing a method of preventing Alzheimer’s disease (AD). Tau contains a microtubule binding domain (MBD) consisting of three or four repeats of 31 and 32 similar residues in its C-terminal half. Although the key event in tau aggregation has been considered to be the formation of β-sheet structures from a short hexapeptide 306 VQIVYK 311 in the third repeat of MBD, its aggregation pathway to filament formation differs between the three- and four-repeated MBDs, owing to the intermolecular and intramolecular disulphide bond formations, respectively. Therefore, the elucidation of a common structural element necessary for the self-assembly of three-/four-repeated full-length tau is an important research subject. Expanding the previous results on the aggregation mechanism of MBD, in this paper, we report that the C–H ... interaction between the Ile308 and Tyr310 side chains in the third repeat of MBD is indispensable for the self-assembly of three-/four-repeated full-length tau, where the interaction provides a conformational seed for triggering the molecular association. On the basis of the aggregation behaviours of a series of MBD and full-length tau mutants, a possible self-association model of tau is proposed and the relationship between the aggregation form (filament or granule) and the association pathway is discussed.
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  • 81
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-08-29
    Beschreibung: The lymphatic vascular system, also known as the second vascular system in vertebrates, plays crucial roles in various physiological and pathological processes. It participates in the maintenance of normal tissue fluid balance, trafficking of the immune cells and absorption of fatty acids in the gut. Furthermore, lymphatic system is associated with the pathogenesis of a number of diseases, including lymphedema, inflammatory diseases and tumour metastasis. Lymphatic vessels are comprised of lymphatic endothelial cells (LECs), which are differentiated from blood vascular endothelial cells. This review highlights recent advances in our understanding of the transcriptional control of LEC fate determination and reflects on efforts to understand the roles of transcriptional networks during this discrete developmental process.
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  • 82
    Publikationsdatum: 2012-08-29
    Beschreibung: The Mediator complex consists of more than 20 subunits. This is composed of four modules: head, middle, tail and CDK/Cyclin. Importantly, Mediator complex is known to play pivotal roles in transcriptional regulation, but its molecular mechanisms are still elusive. Many studies, including our own, have revealed that CDK8, a kinase subunit of the CDK/Cyclin module, is one of the key subunits involved in these roles. Additionally, we previously demonstrated that a novel CDK component, CDK19, played similar roles. It is assumed that various factors that directly affect transcriptional regulation target these two CDKs; thus, we conducted yeast two-hybrid screenings to isolate the CDK19-interacting proteins. From a screening of 40 million colonies, we obtained 287 clones that provided positive results encoded mRNAs, and it turned out that 59 clones of them encoded nuclear proteins. We checked the reading frames of the candidate clones and obtained three positive clones, all of which encoded the transcriptional cofactors, Brahma-related gene 1, B-cell CLL/lymphoma 6 and suppressor of zeste 12 homolog. Intriguingly, these three cofactors are also related to chromatin regulation. Further studies demonstrated that those could bind not only to CDK19 but also to CDK8. These results help elucidate the functional mechanism for the mutual regulations between transcription and chromatin.
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    Thema: Biologie , Chemie und Pharmazie
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  • 83
    Publikationsdatum: 2012-08-29
    Beschreibung: The metalloprotease ADAMTS13 affects platelet adhesion and aggregation through depolymerization of von Willebrand factor (VWF) multimers. Identification of ADAMTS13-binding proteins would reveal the hitherto unrecognized mechanisms underlying microvascular thrombus. To identify ADAMTS13-binding proteins, we performed a yeast two-hybrid screen using the Cys-rich and spacer domains of ADAMTS13, the critical regions for the binding and cleavage of VWF, as a bait region. We identified Lys-plasminogen, an amino-terminal truncated form of plasminogen, as the binding protein to ADAMTS13. Intact Glu-plasminogen did not bind to ADAMTS13. Active-site blocked Lys-plasmin bound to ADAMTS13. Domain truncation of ADAMTS13 and elastase digest of plasminogen indicated that the Cys-rich and spacer domains of ADAMTS13 and the kringle 5 and protease domains of plasminogen served as the main binding sites. Biacore measurements revealed that Lys-plasminogen bound to ADAMTS13 with a K d of 1.9 ± 0.1 x 10 –7 M and Glu-plasminogen exhibited a significantly lower affinity to ADAMTS13. Specific activity measurements revealed that ADAMTS13 and Lys-plasmin were still active even after the binary complex was formed. The binding of ADAMTS13 to Lys-plasminogen may play an important role to localize these two proteases at sites of thrombus formation or vascular injury where the fibrinolytic system is activated.
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  • 84
    Publikationsdatum: 2012-08-29
    Beschreibung: It is generally accepted that the mitochondria play central roles in energy production of most eukaryotes. In contrast, it has been thought that Plasmodium spp., the causative agent of malaria, rely mainly on cytosolic glycolysis but not mitochondrial oxidative phosphorylation for energy production during blood stages. However, Plasmodium spp. possesses all genes necessary for the tricarboxylic acid (TCA) cycle and most of the genes for electron transport chain (ETC) enzymes. Therefore, it remains elusive whether oxidative phosphorylation is essential for the parasite survival. To elucidate the role of TCA metabolism and ETC in malaria parasites, we deleted the gene for flavoprotein (Fp) subunit, Pbsdha , one of four components of complex II, a catalytic subunit for succinate dehydrogenase activity. The Pbsdha(-) parasite grew normally at blood stages in mouse. In contrast, ookinete formation of Pbsdha(-) parasites in the mosquito stage was severely impaired. Finally, Pbsdha(-) ookinetes failed in oocyst formation, leading to complete malaria transmission blockade. These results suggest that malaria parasite may switch the energy metabolism from glycolysis to oxidative phosphorylation to adapt to the insect vector where glucose is not readily available for ATP production.
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  • 85
    facet.materialart.
    Unbekannt
    Oxford University Press
    Publikationsdatum: 2012-08-29
    Beschreibung: Yes-associated protein (YAP) has been shown to play a critical role in the growth of various tumours. Phosphorylation of Ser127 of YAP leads to the inhibition of YAP translocation into nucleus and subsequent failure to regulate the expression of target genes that induce cell proliferation. Chemical manipulation of YAP localization or expression may provide an efficient method for cancer treatment. In a recent work published by Bao et al. ( J. Biochem . 2011;150:199–208), various compounds were screened in human osteosarcoma cells that stably express Green Fluorescent Protein-labeled YAP by monitoring subcellular localization of GFP-YAP. Using this cell-based assay, they found that dobutamine, a β-adrenergic receptor agonist, attenuated YAP-dependent transcription by inhibiting its nuclear translocation. The authors suggest dobutamine as a possible drug for cancer treatment.
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  • 86
    Publikationsdatum: 2012-08-29
    Beschreibung: In the N-terminal domain of thermolysin, two anti-parallel β-strands, Asn112–Ala113–Phe114–Trp115 and Ser118–Gln119–Met120–Val121–Tyr122 are connected by an Asn116–Gly117 turn to form a β-hairpin structure. In this study, we examined the role of Asn116 in the activity and stability of thermolysin by site-directed mutagenesis. Of the 19 Asn116 variants, four (N116A, N116D, N116T and N116Q) were produced in Escherichia coli , by co-expressing the mature and pro domains separately, while the other 15 were not. In the hydrolysis of N -[3-(2-furyl)acryloyl]-glycyl- l -leucine amide (FAGLA) at 25°C, the intrinsic k cat / K m value of N116D was 320% of that of the wild-type thermolysin (WT), and in the hydrolysis of N -carbobenzoxy- l -aspartyl- l -phenylalanine methyl ester (ZDFM) at pH 7.5 at 25°C, the k cat / K m value of N116D was 140% of that of WT, indicating that N116D exhibited higher activity than WT. N116Q exhibited similar activity as WT, and N116A and N116T exhibited reduced activities. The first-order rate constants, k obs , of the thermal inactivation at 80°C were in the order N116A, N116D, N116T 〉 N116Q 〉 WT at all CaCl 2 concentrations examined (1–100 mM), indicating that all variants exhibited reduced stabilities. These results suggest that Asn116 plays an important role in the activity and stability of thermolysin presumably by stabilizing this β-hairpin structure.
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  • 87
    Publikationsdatum: 2012-08-29
    Beschreibung: The Myxococcus xanthus protein phosphatase Pph3 belongs to the Mg 2+ - or Mn 2+ -dependent protein phosphatase (PPM) family. Bacterial PPMs contain three divalent metal ions and a flap subdomain. Putative metal- or phosphate-ion binding site-specific mutations drastically reduced enzymatic activity. Pph3 contains a cyclic nucleotide monophosphate (cNMP)-binding domain in the C-terminal region, and it requires 2-mercaptoethanol for phosphatase activity; however, the C-terminal deletion mutant showed high activity in the absence of 2-mercaptoethanol. The phosphatase activity of the wild-type enzyme was higher in the presence of cAMP than in the absence of cAMP, whereas a triple mutant of the cNMP-binding domain showed slightly lower activities than those of wild-type, without addition of cAMP. In addition, mutational disruption of a disulphide bond in the wild-type enzyme increased the phosphatase activity in the absence of 2-mercaptoethanol, but not in the C-terminal deletion mutant. These results suggested that the presence of the C-terminal region may lead to the formation of the disulphide bond in the catalytic domain, and that disulphide bond cleavage of Pph3 by 2-mercaptoethanol may occur more easily with cAMP bound than with no cAMP bound.
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  • 88
    Publikationsdatum: 2012-08-29
    Beschreibung: Emerging evidence indicates that vitamin D (VD) is an important modulator of brain development and function. To investigate whether VD modulates neurosteroid biosynthesis in neural cells, we investigated the effect of VD 3 on steroidogenic gene expression in human glioma GI-1 cells. We found that VD 3 enhanced CYP11A1 and 3β-hydroxysteroid dehydrogenase gene expression. The induction of CYP11A1 gene expression by VD 3 was dose- and incubation time-dependent. Calcipotriol, a VD 3 receptor (VDR) agonist, also induced CYP11A1 gene expression in GI-1 cells, indicating that VDR is involved in this induction. The induction of progesterone (PROG) de novo synthesis was observed along with the induction of steroidogenic genes by VD 3 . Furthermore, VD 3 enhanced all- trans retinoic acid (ATRA)-induced CYP11A1 gene expression and PROG production. This suggests cooperative regulation of steroidogenic gene expression by the two fat-soluble vitamins, A and D. In addition, a mixed culture of neuronal IMR-32 cells and GI-1 cells treated with ATRA and VD 3 resulted in the induction of PROG-responsive gene expression in the IMR-32 cells. This result shows a paracrine action of PROG that is induced in and released by the GI-1 cells. The relationship between neurological dysfunction associated with VD deficiency and neurosteroid induction by VD is discussed.
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  • 89
    Publikationsdatum: 2012-08-22
    Beschreibung: Rehberg-Haas, S., Hammer, C., Hillgruber, N., Hüssy, K., and Temming, A. 2012. Otolith microstructure analysis to resolve seasonal patterns of hatching and settlement in western Baltic cod. – ICES Journal of Marine Science, 69: . Previous studies showed that Baltic cod ( Gadus morhua ) settle to demersal life at a given size, while the annulus is formed seasonally, irrespective of size. The goal of this study was to examine the timing of check formation in juvenile Baltic cod otoliths to validate macrostructural ageing and to differentiate between true annuli and secondary structures such as settlement checks. Otoliths were collected from fish off Fehmarn Island in 2008 and 2009, and were examined for macrostructural and microstructural patterns using light and scanning electron microscopy. All fish examined were age-0. Back-calculation of hatch dates indicated hatching from April to June and from February to August in 2008 and 2009, respectively. Juveniles formed either one or two translucent rings. The first translucent ring started to form ~3 months post-hatch and was interpreted as a settlement check, since it appeared to be a function of age and/or size and not season. Deposition of the second ring began in mid October to early November irrespective of fish size and/or age, thus indicating that this ring may represent the first annulus of Baltic cod. Both rings were clearly distinguishable in individuals hatched between February and May, but were merged in those fish where settlement coincided with the seasonally formed second ring.
    Print ISSN: 1054-3139
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 90
    Publikationsdatum: 2012-08-22
    Beschreibung: Nash, R. D. M., Wright, P. J., Matejusova, I., Dimitrov, S. P., O'Sullivan, M., Augley, J., and Höffle, H. 2012. Spawning location of Norway pout ( Trisopterus esmarkii Nilsson) in the North Sea. – ICES Journal of Marine Science, 69: . The northern region of the North Sea (56–62°N) was sampled in February/March 2009 for eggs and in May 2010 for larvae. To aid in the identification of Norway pout stage I eggs and distinguish them from other ‘cod-like’ eggs, a Taq-Man probe was designed for this species and used here. Stage I Norway pout egg diameters collected from the field were in the range 1.03–1.28 mm and largely overlapped with the size range determined for whiting ( Merlangius merlangus ). The distribution of Norway pout stage I eggs in 2009 revealed the distribution of spawning in the North Sea and showed that it was similar to the distribution of 2 + Norway pout taken during the International Bottom Trawl Surveys (IBTS) over the same period covering the whole North Sea. The larvae sampled in 2010 were largely in the same area; however, larger larvae occurred to the south-east of the survey area, suggesting advection of young stages from the principal spawning areas in the north-western North Sea to the south-east and toward the Skagerrak.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 91
    Publikationsdatum: 2012-08-22
    Beschreibung: Borrell, Y. J., Piñera, J. A., Sanchez Prado, J. A., and Blanco, G. 2012. Mitochondrial DNA and microsatellite genetic differentiation in the European anchovy Engraulis encrasicolus L. – ICES Journal of Marine Science, 69: . The European anchovy Engraulis encrasicolus is currently one of the principal target species for commercial fisheries in Europe, and most stocks are overfished at present. In this work, specimens were sampled in the Bay of Biscay (Cantabrian, Basque Country, and French coasts) in 2009 and also in the Mediterranean (Adriatic Sea). Mitochondrial DNA (mtDNA; cytochrome b and 16S) was sequenced, and 14 nuclear microsatellites showing high and low levels of polymorphism were arranged in three multiplex polymerase chain reaction (PCR) systems and genotyped. Two main ancient mitochondrial clades were found. These clades are separated by 15 mutational steps and 1.7% sequence divergence, corresponding to a separation time of ~0.5 million years ago. Our results using both mtDNA and microsatellites suggest the presence of at least three genetically differentiated groups: the west Cantabrian Sea, the rest of the populations in the Bay of Biscay, and the Mediterranean. Although it is known that western Iberian Atlantic populations of E. encrasicolus may be genetically different from those of the Bay of Biscay, the results suggest that the transition between these groups may be as close as a 100 km and that a recent genetic homogenization process in the eastward area of the Bay of Biscay has probably occurred.
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    Publiziert von Oxford University Press
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  • 92
    Publikationsdatum: 2012-08-22
    Beschreibung: Armannsson, H., and Jónsson, S. Þ. 2012. Vertical migrations of saithe ( Pollachius virens ) in Icelandic waters as observed with data storage tags. – ICES Journal of Marine Science, 69: . Knowledge of fish behaviour is important for general understanding of fish life history and responses to environmental and biological conditions. Vertical migrations can affect bottom trawl indices of the fish through their availability to the trawl and acoustic indices through changes in target strength. Here we present results based on data sets of hourly recordings of depth from 51 saithe. The data were analysed with respect to diel and seasonal differences in saithe vertical movement. Duration and extent of vertical movements were analysed with 1 min resolution, and the daily vertical range (DVR) was analysed with respect to potential use of the free vertical range (FVR). Our results show diel and seasonal differences in hourly depth changes of saithe, indicating reduced activity during night-time and in winter. Individual saithe are capable of rapid depth changes in 〈1 min, often of the same order as the maximum observed in movements of longer duration. The DVR often exceeded the FVR, and in some instances exceeded critical limits, indicating that saithe may at times only maintain neutral buoyancy near the upper limit of their daily vertical range.
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  • 93
    Publikationsdatum: 2012-08-22
    Beschreibung: Hvingel, C., Kingsley, M.C.S., and Sundet, J.H. 2012. Survey estimates of king crab ( Paralithodes camtschaticus ) abundance off Northern Norway using GLMs within a mixed generalized gamma-binomial model and Bayesian inference. – ICES Journal of Marine Science, 69: . A trawl survey provides information on number and biomass of introduced king crab ( Paralithodes camtschaticus ) to the management of a fishery off the coast of Northern Norway; the annual catch quotas are largely set as a percentage of the survey estimate. A specially built sledge trawl was designed for the survey. It needs only small areas of trawlable bottom, performs well on a wide range of bottoms, and appears to have good catchability for benthic organisms. Many survey hauls catch no crabs and the non-zero catches have a highly skewed distribution. Data were therefore analysed with a compound model, in which separate predictors were fitted for the proportion of zero catches and for the catch size of the non-zero catches. The compound model was fitted by Bayesian methods using WinBUGS. The distribution of non-zero catches fitted well to a generalized gamma distribution, but with parameter values that made it approximate a lognormal distribution. Numbers of fishable crabs peaked in 2003, and total numbers in 2010 were about two-fifths of the 2003 maximum.
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  • 94
    Publikationsdatum: 2012-08-22
    Beschreibung: Amandè, M. J., Chassot, E., Chavance, P., Murua, H., Delgado de Molina, A., and Bez, N. 2012. Precision in bycatch estimates: the case of tuna purse-seine fisheries in the Indian Ocean. – ICES Journal of Marine Science, 69: . Estimating bycatch, i.e. the incidental catch of non-target marine animals and undersized individuals of target species, by raising observer data to the whole fishery is routine practice. The annual bycatch of the European tropical tuna purse-seine fishery over the period 2003–2009 was estimated at 11 590 t [95% confidence interval: (8165–15 818 t)], corresponding to 4.7% of the tuna landings. An analysis of the variability in the precision of this estimate, based on generalized linear models and Monte Carlo simulations, showed that the current sampling coverage of the tropical tuna fishery observer programme, which is 4.6% of the fishing trips, resulted in large uncertainties in bycatch estimates by species, i.e. none of the estimates have a relative root mean square error smaller than 50%. Although the overall magnitude of bycatch of the fishery appeared to be small, the current sampling coverage was insufficient to give any reliable estimate for low-occurring species, such as marine turtles, some oceanic pelagic sharks, and some billfishes. Increasing the sampling coverage would likely improve bycatch estimates. Simulation outputs were produced to help define (i) trade-offs between the priority species to be monitored, (ii) the estimation precision, (iii) expected accuracy, and (iv) the associated sampling costs.
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  • 95
    Publikationsdatum: 2012-08-22
    Beschreibung: Furness, R. W., Wade, H. M., Robbins, A. M. C., and Masden, E. A. 2012. Assessing the sensitivity of seabird populations to adverse effects from tidal stream turbines and wave energy devices. – ICES Journal of Marine Science, 69: . Tidal turbines and wave energy devices may affect seabird populations through collision mortality, disturbance and habitat loss. Given the pressures to harness tidal and wave energy, especially in Scottish waters, there is an urgent need to assess population-level impacts on seabird species. With a lack of deployed devices to monitor in areas of importance for seabirds, our approach uses data from scientific literature on seabird ecology and conservation importance likely to influence population vulnerability to "wet renewables" in Scottish waters. At this stage however, we can only infer likely interactions with tidal and wave devices. We identify black guillemot, razorbill, European shag, common guillemot, great cormorant, divers and Atlantic puffin as the species most vulnerable to adverse effects from tidal turbines in Scottish waters. We identify divers as the species most vulnerable to adverse effects from wave energy devices in Scottish waters. Wave energy devices seem likely to represent a lesser hazard to seabirds than tidal turbines, and both forms of energy capture seem likely to represent a lower hazard to seabirds than offshore wind farms (wind-power plants). The indices developed here for Scottish seabird populations could be applied to populations elsewhere. This approach will help in identifying likely impacts of tidal and wave energy deployments on seabirds, and in optimizing deployment of resources for compulsory environmental monitoring.
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  • 96
    Publikationsdatum: 2012-08-22
    Beschreibung: Rogers, P. J., Huveneers, C., Page, B., Hamer, D. J., Goldsworthy, S. D., Mitchell, J. G., and Seuront, L. 2012. A quantitative comparison of the diets of sympatric pelagic sharks in gulf and shelf ecosystems off southern Australia. – ICES Journal of Marine Science, 69: . Predator–prey dynamics represent an important determinant in the functioning of marine ecosystems. This study provides the first quantitative investigation of the diets of sympatric pelagic shark species in gulf and shelf waters off southern Australia. Stomachs of 417 sharks collected from fishery catches between 2007 and 2011 were examined, including 250 bronze whalers, 52 shortfin makos, 49 dusky sharks, 39 smooth hammerheads, and 27 common threshers. Dusky sharks had the highest dietary diversity of the five species examined. We found overlap in the consumption of cephalopods, small pelagic teleosts, crustaceans, and benthic teleosts in bronze whalers, dusky sharks, and smooth hammerheads, and preliminary evidence of specialization in the highly migratory species, the common thresher and the shortfin mako. Findings were discussed and compared with previous studies in other temperate marine ecosystems. This study will significantly improve the understanding of the ecological roles of these top predators in the gulf and shelf habitats off southern Australia, and enhance the ecosystem models being developed for this unique bioregion.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 97
    Publikationsdatum: 2012-08-22
    Beschreibung: Roa-Ureta, R. H. 2012. Modelling in-season pulses of recruitment and hyperstability-hyperdepletion in the Loligo gahi fishery around the Falkland Islands with generalized depletion models. – ICES Journal of Marine Science, 69: . The time-series of daily catch, fishing effort, and mean body mass of the summer fishing seasons of the squid, Loligo gahi , in the Falkland Islands from 1990 to 2009 are investigated with generalizations of depletion models that account for in-season pulses of recruitment (i.e. open populations) and non-linear relationships between catch as the response variable, and effort and abundance as the predictor variables. Two main results were found. First, stock dynamics are more complex than assumed by Leslie–Davis or De Lury depletion models, because in most years, there are several major in-season recruitment pulses, sometimes even larger than the pre-season pulse, contradicting the basic assumption of a decline in catch rates over the fishing season. Second, the fishery operates under a regime of hyperstability—catch rates decline slower than abundance—at low stock abundance and hyperdepletion—catch rates decline faster than abundance—at intermediate and high stock abundance. The hyperdepleted regime is far more prevalent, a result attributed to the availability of refuges from fishing operations, which may lower the abundance threshold to pass from the low-abundance hyperstable regime to the higher-abundance hyperdepleted regime.
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    Publiziert von Oxford University Press
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  • 98
    Publikationsdatum: 2012-08-22
    Beschreibung: Øigård, T. A., Frie, A. K., Nilssen, K. T., and Hammill, M. O. 2012. Modelling the abundance of grey seals ( Halichoerus grypus ) along the Norwegian coast. – ICES Journal of Marine Science, 69: . An age-structured population dynamics model of the Norwegian grey seal ( Halichoerus grypus ) population has been developed. The model is of a Bayesian character in the sense that priors for various parameters were used. Model runs indicated an increase in the abundance of the total Norwegian grey seal population during the last 30 years, suggesting a total of 8740 (95% confidence interval: 7320–10 170) animals in 2011. A total catch of 707 (95% confidence interval: 532–882) grey seals would maintain the population size at the 2011 level. Model runs suggest that current catch levels will likely result in a reduction in the population size in Sør-Trøndelag and Nord-Trøndelag counties, and an increase in the population size in Rogaland, Nordland, Troms, and Finnmark counties. The model runs assumed that 80% of the seals taken in Rogaland came from the UK and that 50 and 55% of the catches in Troms and Finnmark, respectively, were immigrants from Russia.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 99
    Publikationsdatum: 2012-08-22
    Beschreibung: González-Irusta, J. M., Punzón, A., and Serrano, A. 2012. Environmental and fisheries effects on Gracilechinus acutus (Echinodermata: Echinoidea) distribution: is it a suitable bioindicator of trawling disturbance? – ICES Journal of Marine Science, 69: . Habitat preferences of Gracilechinus acutus in the southern Bay of Biscay were studied using data from autumn bottom-trawl surveys. Wet weight and number of specimens of G. acutus were obtained and related to environmental variables (depth, sediment type, and organic matter percentage) at each haul and to trawl fishing effort. With this information and the otter trawl effort data, the environmental requirements and the impact of the trawl fishery on G. acutus populations were analysed. Although the species was present in all depth strata and all sediment types studied, it had clear habitat preferences, as greater abundances and mean weight values were found at depths ranging from 71 to 200 m and in bottom sediments dominated by coarse and medium sands. The effect of disturbance by trawling on this echinoid was significant and clearly negative. Seabeds exposed to higher trawling disturbance showed lower values of urchin abundance and smaller urchins than areas with lower disturbance. Results of the present study confirm the initial hypothesis of the suitability of using this urchin as a bioindicator of trawling impact but only in areas with appropriate environmental conditions, highlighting the importance of attaining a wider knowledge on the essential habitat of the species.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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  • 100
    Publikationsdatum: 2012-08-22
    Beschreibung: Gårdmark, A., Östman, Ö., Nielsen, A., Lundström K., Karlsson O., Pönni, J., and Aho, T. 2012. Does predation by grey seals ( Halichoerus grypus ) affect Bothnian Sea herring stock estimates? – ICES Journal of Marine Science, 69: . Mortality of small pelagic fish due to marine mammals is generally considered to be low compared with other sources of mortality. With recent recoveries of marine mammal predators worldwide, this may no longer hold. The grey seal ( Halichoerus grypus ) population in the Bothnian Sea has increased fivefold since 1985. Its main prey, herring ( Clupea harrengus ), is a key species for fisheries in the region. Yet, current stock assessments assume constant natural mortality, leading to a risk of biased stock estimates with increasing predation and misleading analyses of herring population dynamics. We estimated grey seal predation from diet data and reanalysed herring spawning stock biomass (SSB) during 1973–2009. Accounting for predation increased the herring SSB 16% (maximum 19%), but this was within the confidence intervals when ignoring predation. Although mortality in older individuals was inflated when accounting for seal predation, this did not change the conclusions about drivers of herring dynamics. Accounting for grey seal predation is important for abundance estimates of old herring, but currently not for SSB estimates, given the great uncertainties in the standard assessment. The grey seal impact on Bothnian Sea herring will need to be reassessed if stock age composition, grey seal feeding preferences, or total stock development change.
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    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Oxford University Press
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