ALBERT

Beschreibung:    It has been shown that the description of the details of the electronic spectra obtained by the combination of the dynamical mean field theory, quantum Monte Carlo methods, and the maximum entropy method can be significantly improved by changing the last method to optimal regularization of the analytic continuation of the Green’s function to the real frequency axis. Starting with the quantum Monte Carlo data, this method has reconstructed peaks in the structure of Hubbard subbands with a maximum error of 0.001 to 0.01. Owing to the universality of the quantum Monte Carlo method, by varying hybridization, it is possible to determine the features of the hybridized function that are responsible for the formation of the structure of Hubbard subbands. It has been shown that there is no direct relation between the peak structure of subbands and the central Kondo peak. The result indicates the charge nature of resonances responsible for the formation of the peak structure. Content Type Journal Article Category Methods of Theoretical Physics Pages 768-773 DOI 10.1134/S0021364011220073 Authors I. S. Krivenko, Faculty of Physics, Moscow State University, Moscow, 119992 Russia A. N. Rubtsov, Faculty of Physics, Moscow State University, Moscow, 119992 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 94 Journal Issue Volume 94, Number 10

Beschreibung:    The spallation of a nanometer-thick melt layer on a GaAs surface during its ablation by femtosecond laser pulses occurs with subnanosecond delays and lift-off velocities that depend on the laser fluence after its complete thermal (hydrodynamic) expansion/acoustic relaxation. The position of the spall interface in the melt is determined by the depth of the formation of a two-dimensional subsurface layer of nanobubbles (nanofoam), whereas the strongly heated surface layer of the melt above the nanofoam is partially removed in the form of a vapor-drop mixture. At the thermal expansion stage, acoustic reverberations are observed in the melt layer and characterize both the dynamics of an increase in its thickness and the shift of the cavitation region (nanofoam) inside the melt. Moreover, these reverberations can additionally stimulate spallation, promoting cavitation in the completely unloaded melt in the case of passage of a weak rarefaction wave. Content Type Journal Article Category Condensed Matter Pages 753-758 DOI 10.1134/S002136401122005X Authors A. A. Ionin, Lebedev Physical Institute, Russian Academy of Sciences, Leninskii pr. 53, Moscow, 119991 Russia S. I. Kudryashov, Lebedev Physical Institute, Russian Academy of Sciences, Leninskii pr. 53, Moscow, 119991 Russia L. V. Seleznev, Lebedev Physical Institute, Russian Academy of Sciences, Leninskii pr. 53, Moscow, 119991 Russia D. V. Sinitsyn, Lebedev Physical Institute, Russian Academy of Sciences, Leninskii pr. 53, Moscow, 119991 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 94 Journal Issue Volume 94, Number 10

Beschreibung:    The sublimation of glycyl-L-α-alanine (Gly-Ala), L-α-alanyl-L-α-alanine (Ala-Ala), and DL-α-alanyl-DL-α-valine (Ala-Val) aliphatic dipeptides is studied by electron ionization mass spectrometry in combination with Knudsen effusion. The temperature range in which substances sublime as monomer molecular forms is determined. Enthalpies of sublimation Δ s H °( T ) are determined for Gly-Ala, Ala-Ala, and Ala-Val. It is shown that the enthalpy of sublimation of dipeptides increases with an increase in the side hydrocarbon radical. The unknown Δ s H °(298) values for 17 amino acids and nine dipeptides are estimated using the proposed “structure-property” correlation model, in which the geometry and electron characteristics of molecules are used as structural descriptors. Content Type Journal Article Category Physical Chemistry of Surface Phenomena Pages 457-462 DOI 10.1134/S0036024412030065 Authors V. G. Badelin, Institute of Chemistry of Solutions, Russian Academy of Sciences, Ivanovo, 153045 Russia E. Yu. Tyunina, Ivanovo State University of Chemical Technology, Ivanovo, 153000 Russia A. V. Krasnov, Ivanovo State University of Chemical Technology, Ivanovo, 153000 Russia V. V. Tyunina, Ivanovo State University of Chemical Technology, Ivanovo, 153000 Russia N. I. Giricheva, Ivanovo State University, Ivanovo, 153025 Russia A. V. Girichev, Ivanovo State University of Chemical Technology, Ivanovo, 153000 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 3

Beschreibung:    The acid-base properties of metal acetylacetonates and chromatographic sorbents on the basis of Chromaton N with deposited combined stationary phases from polyethylene glycol (PEG 20M) and nickel(II) and iron(III) acetylacetonates have been investigated by means of pH measurement and Hammett indicator adsorption. The change of the acid-base state of the surface of Chromaton N depending on the nature of a metal of the modifying additive, the complex structure, and the deposition method has been demonstrated. Content Type Journal Article Category Physical Chemistry of Surface Phenomena Pages 463-467 DOI 10.1134/S0036024412030272 Authors Yu. G. Slizhov, Tomsk State University, Tomsk, 634050 Russia T. N. Matveev, Tomsk State University, Tomsk, 634050 Russia T. S. Minakova, Tomsk State University, Tomsk, 634050 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 3

Beschreibung:    The properties of the porous polymer Dowex L-285, modified by 5-hydroxy-6-methyluracil, are investigated by means of gas chromatography. It is found that the modification leads to a considerable increase in the sorption activity of the porous polymer with respect to both polar and nonpolar molecules. It is shown that each type of cavities in the supramolecular structure of 5-hydroxy-6-methyluracil contributes to the adsorption and thermodynamic properties of the modified adsorbent. Content Type Journal Article Category Physical Chemistry of Separation Processes: Chromatography Pages 475-478 DOI 10.1134/S0036024412030132 Authors V. Yu. Gus’kov, Bashkir State University, Ufa, 450074 Russia S. P. Ivanov, Institute of Organic Chemistry, Ufa Scientific Center, Russian Academy of Sciences, Ufa, 450054 Russia R. A. Khabibullina, Bashkir State University, Ufa, 450074 Russia R. R. Garafutdinov, Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Ufa, 450054 Russia F. Kh. Kudasheva, Bashkir State University, Ufa, 450074 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 3

Beschreibung:    A planar homogeneous waveguide array with two-level systems has been considered. The evolution of an electromagnetic field is described by the Maxwell-Bloch equations taking into account the action of the fields of neighboring waveguides beyond the slow-envelope approximation. It has been shown that the model in the continuous approximation is reduced to an integrable system of equations, including the case of a nonzero static dipole moment. The model can be used to analyze the nonlinear mechanism of the compression of pulses and the conditions of overcoming of the diffraction limit. Content Type Journal Article Category Optics and Laser Physics Pages 837-839 DOI 10.1134/S0021364011240106 Authors A. A. Zabolotskii, Institute of Automatics and Electrometry, Siberian Branch, Russian Academy of Sciences, Universitetskii pr. 1, Novosibirsk, 630090 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 94 Journal Issue Volume 94, Number 12

Beschreibung:    In this research a new idea for prediction of ultimate sizes of bimetallic nanocomposites synthesized in water-in-oil microemulsion system is proposed. In this method, by modifying Tabor Winterton approximation equation, an effective Hamaker constant was introduced. This effective Hamaker constant was applied in the van der Waals attractive interaction energy. The obtained effective van der Waals interaction energy was used as attractive contribution in the total interaction energy. The modified interaction energy was applied successfully to predict some bimetallic nanoparticles, at different mass fraction, synthesized in microemulsion system of dioctyl sodium sulfosuccinate (AOT)/isooctane. Content Type Journal Article Category Physical Chemistry of Nanoclusters and Nanomaterials Pages 2014-2017 DOI 10.1134/S003602441213002X Authors Alireza Salabat, Department of Chemistry, Faculty of Science, Arak University, P. O. Box 38156-8-8349, Arak, Iran Hassan Saydi, Department of Chemistry, Faculty of Science, Arak University, P. O. Box 38156-8-8349, Arak, Iran Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    Densities, viscosities, refractive indices and ultrasonic velocities of the binary mixtures of acetophenone with ethyl acetate were measured over the entire mole fractions at 303.15, 313.15, and 323.15 K. From the experimental results, excess molar volumes V E , viscosity deviation Δ η , refractive index deviation Δ n D , deviations in isentropic compressibility Δκ s and excess intermolecular free length Δ L f are calculated. The viscosity values were fitted to the models of Krishnan-Laddha and McAllister. The thermophysical properties under study were fit to the Jouyban-Acree model. The excess values were correlated using Redlich-Kister polynomial equation to obtain their coefficients and standard deviations. The data obtained fitted with the values correlated by the corresponding models very well. The results are interpreted in terms of molecular interactions occurring in the solution. Content Type Journal Article Category Physical Chemistry of Solutions Pages 1947-1952 DOI 10.1134/S0036024412130195 Authors K. Saravanakumar, Department of Chemical Engineering, Sathyabama University, Chennai, 600119 India R. Baskaran, Department of Chemical Engineering, St. Joseph’s College of Engineering, Chennai-119, India T. R. Kubendran, Department of Chemical Engineering, A.C. College of Technology, Anna University, Chennai, 600025 India Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    The solubilities in the KCl-MgCl 2 -H 2 O system were determined at 50 and 75°C and the phase diagrams and the diagram of refractive index vs composition were plotted. Two invariant point, three univariant curves, and three crystallization zones, corresponding to potassium chloride, hexahydrate (MgCl 2 · 6H 2 O) and double salt (KCl · MgCl 2 · 6H 2 O) showed up in the phase diagrams of the ternary system, The mixing parameters θ K, Ca and Ψ K, Ca, Cl and equilibrium constant K sp were evaluated in KCl-MgCl 2 -H 2 O system by least-squares optimization procedure, in which the single-salt Pitzer parameters of KCl and MgCl 2 β (0) , β (1) , β (2) , and C ϕ were directly calculated from the literature. The results obtained were in good agreement with the experimental data. Content Type Journal Article Category Physical Chemistry of Solutions Pages 1930-1935 DOI 10.1134/S0036024412130146 Authors Ji-min Yang, School of Chemistry & Resources and Environment, Linyi University, Linyi, 276005 China Jing Peng, School of Chemistry & Resources and Environment, Linyi University, Linyi, 276005 China Yu-xia Duan, School of Chemistry & Resources and Environment, Linyi University, Linyi, 276005 China Chong Tian, School of Chemistry & Resources and Environment, Linyi University, Linyi, 276005 China Mei Ping, Experimental Centre, Linyi University, Linyi, 276005 China Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    The insertion reactions of the germylenoid H 2 GeClMgCl with RH (R = F, OH, NH 2 ) have been studied by using the DFT B3LYP and QCISD methods. The geometries of the stationary points on the potential energy surfaces of the reactions were optimized at the B3LYP/6-311+G( d , p ) level of theory. The calculated results indicate that all the mechanisms of the three insertion reactions are identical to each other. The QCISD/6-311++G( d , p )//B3LYP/6-311+G( d , p ) calculated potential energy barriers for the three insertion reactions of R = F, OH, and NH 2 are 164.62, 193.30, and 200.73 kJ mol −1 , and the reaction energies for the three reactions are −57.46, −35.65, and −22.22 kJ mol −1 , respectively. Under the same situation, the insertion reactions should occur easily in the following order H-F 〉 H-OH 〉 H-NH 2 . In THF solvent the insertion reactions get more difficult than in gas phase. Content Type Journal Article Category Structure of Matter and Quantum Chemistry Pages 1969-1973 DOI 10.1134/S0036024412130225 Authors Wen-Zuo Li, College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005 P. R. China Yu-Wei Pei, College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005 P. R. China Jian-Bo Cheng, College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005 P. R. China Qing-Zhong Li, College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005 P. R. China Bao-An Gong, College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005 P. R. China Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    The gas-phase reaction of palladium atom with acetone is investigated using density functional theory. Geometries and energies of the reactants, intermediates, and products involved are calculated. Both ground and excited state potential energy surfaces are investigated in detail. The present results show that the title reaction start with the formation of an η 2 -CH 3 COCH 3 -metal complex, followed by C-O, C-H, and C-C activation. These reactions can lead to four different products (PdO + C 3 H 6 , PdCH 2 COCH 3 + H, PdCH 2 + CH 3 CHO, and PdCOCH 2 + CH 4 ). The present results may be helpful in understanding the mechanism of the title reaction and further experimental investigation of the reaction. Content Type Journal Article Category Structure of Matter and Quantum Chemistry Pages 1982-1990 DOI 10.1134/S0036024412130092 Authors Guo-Liang Dai, School of Pharmaceutical and Chemical Engineering, Taizhou University, Linhai, 317000 China Chuan-Feng Wang, School of Pharmaceutical and Chemical Engineering, Taizhou University, Linhai, 317000 China Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    In this paper, biomimetic synthesis of calcium carbonate (CaCO 3 ) in the presence of biomolecules of two vegetables-tomato and capsicum is investigated. Scanning electron microscopy and X-ray powder diffractometry were used to characterize the CaCO 3 obtained. The biomolecules in the extracts of two vegetables are determined by UV-vis or FTIR. The results indicate that a mixture of calcite and vaterite spheres constructed from small particles is produced with the extract of tomato, while aragonite rods or ellipsoids are formed in the presence of extract of capsicum. The possible formation mechanism of the CaCO 3 crystals with tomato biomolecules can be interpreted by particle-aggregation based non-classical crystallization laws. The proteins and/or other biomolecules in tomato and capsicum may control the formation of vaterite and aragonite crystals by adsorbing onto facets of them. Content Type Journal Article Category Biophysical Chemistry Pages 2071-2075 DOI 10.1134/S003602441213016X Authors Long Chen, Department of Chemistry, Huangshan University, Huangshan, 245041 P. R. China Wang-Hua Xu, School of Chemistry and Chemical Engineering, Anqing Teachers College, Anqing, 246011 P. R. China Ying-Guo Zhao, School of Chemistry and Chemical Engineering, Anqing Teachers College, Anqing, 246011 P. R. China Yan Kang, Department of Chemistry, Huangshan University, Huangshan, 245041 P. R. China Shao-Hua Liu, Department of Chemistry, Huangshan University, Huangshan, 245041 P. R. China Zai-Yong Zhang, Department of Chemistry, Huangshan University, Huangshan, 245041 P. R. China Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    The Er 3+ :Y 3 Al 5 O 12 , an upconversion luminescence agent, which is able to transform the visible light to ultraviolet light, was synthesized by nitrate-citric acid method. And then, a novel photocatalyst, Er 3+ :Y 3 Al 5 O 12 /ZnO composites, was prepared by ultrasonic dispersing and liquid boil method. X-ray diffraction (XRD) and scanning electron microscopy (SEM) were used to characterize the structural morphology and surface properties of the Er 3+ :Y 3 Al 5 O 12 /ZnO. Azo Fuchsine dye was selected as target organic pollutant to inspect the photocatalytic activity of Er 3+ :Y 3 Al 5 O 12 /ZnO. The key parameters affecting the photocatalytic activity of Er 3+ :Y 3 Al 5 O 12 /ZnO, such as Er 3+ :Y 3 Al 5 O 12 content, heat-treatment temperature and heat-treatment time, were studied. In addition, the effects of dye initial concentration, Er 3+ :Y 3 Al 5 O 12 /ZnO amount and solar light irradiation time were also reviewed, as well as the photocatalytic activity in degradation of other organic dyes were compared. It was found that the photocatalytic activity of Er 3+ :Y 3 Al 5 O 12 /ZnO was much superior to pure ZnO under the same conditions. Thus, the Er 3+ :Y 3 Al 5 O 12 /ZnO is a useful photocatalyst for the wastewater treatment because it can efficiently utilize solar light by converting visible light into ultraviolet light. Content Type Journal Article Category Photochemistry and Magnetochemistry Pages 2049-2056 DOI 10.1134/S0036024412130262 Authors L. N. Yin, College of Chemistry, Liaoning University, Shenyang, 110036 P. R. China Y. Li, College of Chemistry, Liaoning University, Shenyang, 110036 P. R. China J. Wang, College of Chemistry, Liaoning University, Shenyang, 110036 P. R. China Y. M. Kong, College of Pharmacy, Liaoning University, Shenyang, 110036 P. R. China Y. Zhai, College of Chemistry, Liaoning University, Shenyang, 110036 P. R. China B. X. Wang, College of Chemistry, Liaoning University, Shenyang, 110036 P. R. China K. Li, College of Chemistry, Liaoning University, Shenyang, 110036 P. R. China X. D. Zhang, College of Chemistry, Liaoning University, Shenyang, 110036 P. R. China Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    In this work, we report the formation of leaf-like ZnO nanoflakes by anodization of zinc foil in a mixture of ammonium sulfate and sodium hydroxide electrolytes under various applied voltage and concentration of sodium hydroxide. The morphology and structure of ZnO nanoflakes were investigated by field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction analysis. In addition, the photocatalytic activity of the prepared nanoflakes zinc oxide was evaluated in the photodegradation of organic dye methylene blue (MB) solution under UV irradiation. It was found that zinc oxide prepared under high concentration of sodium hydroxide and high voltage showed better performance in the photodegradation of methylene blue. Content Type Journal Article Category Photochemistry and Magnetochemistry Pages 2041-2048 DOI 10.1134/S0036024412130171 Authors Muhammad Akhyar Farrukh, Department of Chemistry, GC University Lahore, Lahore, 54000 Pakistan Chin-Kiat Thong, School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia Rohana Adnan, School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia Mohd Amirrul Kamarulzaman, School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 13

Beschreibung:    INMAP was first identified as an interphase nucleus and mitotic apparatus-associated protein that plays essential roles in the formation of the spindle and cell-cycle progression. Here, we report that INMAP might be conserved from prokaryotes to humans, is a truncated version of the RNA polymerase III subunit B POLR3B, and is up-regulated in several human cancer cell lines including HeLa, Bel-7402, HepG2 and BGC-823. Deletion analysis revealed that the 209–290 amino-acid region is necessary for the punctate distribution of INMAP in the nucleus. Furthermore, over-expression of INMAP inhibited the transcriptional activities of p53 and AP-1 in a dose-dependent manner. These results suggest that INMAP may function through the p53 and AP-1 pathways, thus providing a possible link of its activity with tumourigenesis. Integrating our data and those in previous studies, it can be concluded that INMAP plays dual functional roles in the coordination of mitotic kinetics with gene expression as well as in cell-fate determination and proliferation. Content Type Journal Article Pages 1-9 DOI 10.1007/s11010-012-1507-4 Authors Zhou Yunlei, Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing, 100875 People’s Republic of China Chen Zhe, Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing, 100875 People’s Republic of China Lei Yan, Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing, 100875 People’s Republic of China Wang Pengcheng, Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875 People’s Republic of China Zheng Yanbo, The Institute of Medical Biotechnology (IMB) of the Chinese Academy of Medical Sciences, Beijing, 100050 People’s Republic of China Sun Le, AbMax Biotechnology Co., Ltd, Haidian, Beijing, 100085 People’s Republic of China Liang Qianjin, Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing, 100875 People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The increased vascular calcification, cardiovascular morbidity, and mortality in chronic kidney disease (CKD) patients has been associated with disturbances in mineral-bone metabolism. In order to determine markers of the vascular calcification frequently observed in these patients, blood samples of elderly male and female hemodialysis CKD patients were used to measure serum levels of: osteoprotegerin (OPG), total soluble receptor activator of nuclear factor-κB ligand (sRANKL), and fetuin-A by enzyme immunoassay; tartrate-resistant acid phosphatase (TRACP-5b), and bone-specific alkaline phosphatase (BAP) by immunoenzymometric assay; osteocalcin (OC) by ELISA; iPTH by immunoradiometric assay; 25(OH)D 3 and 1,25(OH) 2 D 3 , by I 125 radioimmunoassay; and calcium and phosphorus by photometric assay. Serum OPG, BAP, iPTH, phosphorus, and OC levels were higher and serum 25(OH)D 3 , 1,25(OH) 2 D 3 , and fetuin-A levels lower in both male and female CKD patients than in their respective controls. Our results indicate that the bone formation and resorption parameters are altered in elderly male and female hemodialysis CKD patients. These changes may lead to vascular calcifications and cardiovascular complications, given that elevated OPG and OC levels and reduced fetuin-A levels are associated with cardiovascular events. Content Type Journal Article Pages 1-7 DOI 10.1007/s11010-012-1500-y Authors Alvaro Osorio, Vascular Surgery Unit, Sanitas Hospital, Madrid, Spain Esperanza Ortega, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avenida de Madrid s/n, 18012 Granada, Spain Jesús M. Torres, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avenida de Madrid s/n, 18012 Granada, Spain Pilar Sanchez, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avenida de Madrid s/n, 18012 Granada, Spain Estrella Ruiz-Requena, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avenida de Madrid s/n, 18012 Granada, Spain Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Hereditary haemorrhagic telangiectasia (HHT) is an autosomal dominant disease characterised by vascular dysplasia and increased bleeding that affect 1 in 5,000 people world-wide. Pathology is linked to mutations in genes encoding components of the heteromeric transforming growth factor-beta receptor (TGF-beta) and SMAD signalling pathway. Indeed HHT1 and HHT2 result from mutations in the genes encoding endoglin and activin-like kinase 1 (ALK1), TGF-beta receptor components. However, the fundamental cellular defects underlying HHT is poorly understood. Previously using confocal microscopy and N -glycosylation analysis, we found evidence that defective trafficking of endoglin from the endoplasmic reticulum (ER) to the plasma membrane is a mechanism underlying HHT1 in some patients. In this study, we used confocal microscopy to investigate whether a similar mechanism contributes to HHT2 pathology. To do this we expressed wild-type ALK1 and a number of HHT2 patient mutant variants as C-terminally tagged EGFP fusion proteins and tested their localisation in HeLa cells. We found that wild-type ALK1–EGFP was targeted predominantly to the plasma membrane, as evidenced by its colocalisation with the co-expressed HA-tagged endoglin. However, we found that in the majority of cases analysed the HHT2 patient mutant protein was retained within the ER as indicated by their colocalisation with the ER resident marker (calnexin) and lack of colocalisation with cell surface associated HA-endoglin. We conclude that defective trafficking and retention in the ER of mutant ALK1 protein is a possible mechanism of HHT2 in some patients. Content Type Journal Article Pages 1-11 DOI 10.1007/s11010-012-1496-3 Authors Alistair N. Hume, School of Biomedical Sciences, Queen’s Medical Centre, Nottingham, NG7 2UH UK Anne John, Departments of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al-Ain, United Arab Emirates Nadia A. Akawi, Departments of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al-Ain, United Arab Emirates Aydah M. Al-Awadhi, Departments of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al-Ain, United Arab Emirates Sarah S. Al-Suwaidi, Departments of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al-Ain, United Arab Emirates Lihadh Al-Gazali, Departments of Paediatrics, College of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates Bassam R. Ali, Departments of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al-Ain, United Arab Emirates Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Most studies using a hypercaloric diet to induce obesity have focused on the metabolism of fat and carbohydrates. Less concern has been given to the metabolism of amino acids, despite evidence of modifications in nitrogen metabolism during obesity. The aim of this study was to evaluate amino acid metabolism in livers from cafeteria diet-induced obese rats. Blood parameters were analysed, and histological sections of livers were stained with Sudan III. The enzymatic activities of some enzymes were determined in liver homogenates. Gluconeogenesis, ureagenesis, and oxygen consumption were evaluated in rat livers perfused with glutamine, alanine, or ammonium chloride. Compared to control rats, cafeteria-fed rats demonstrated higher levels of triacylglycerol and glucose in the blood and greater accumulation of fat in livers. Gluconeogenesis and urea production in livers perfused with glutamine and alanine at higher concentrations showed a substantial reduction in cafeteria-fed rats. However, no significant difference was observed among groups perfused with ammonium chloride. The activities of the enzymes alanine aminotransferase, glutaminase, and aspartate aminotransferase in the livers were reduced in cafeteria-fed rats. Taken together, these data are consistent with the hypothesis that livers from cafeteria diet-induced obese rats exhibit a limitation in their maximal capacity to metabolise glutamine and alanine to glucose, ammonia, and urea, not because of an impairment in gluconeogenesis and/or ureagenesis, but rather due to a depression in the activities of enzymes that catalyse the initial steps of amino acid metabolism. Content Type Journal Article Pages 1-13 DOI 10.1007/s11010-012-1499-0 Authors Cristiane Vizioli de Castro Ghizoni, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Fabiana Rodrigues Silva Gasparin, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Antonio Sueiti Maeda Júnior, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Fernando Olinto Carreño, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Rodrigo Polimeni Constantin, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Adelar Bracht, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Emy Luiza Ishii Iwamoto, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Jorgete Constantin, Department of Biochemistry, Laboratory of Liver Metabolism, University of Maringá, Avenida Colombo 5790, 87020-900 Maringá, Paraná, Brazil Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The nasal epithelial barrier dysfunction is associated with the pathogenesis of nasal allergy; the causative factors are to be further elucidated. Ubiquitin E3 ligase TNFIAP3 (TNFIAP3, in short) plays a role in the maintenance of the homeostasis in the body. This study aims to elucidate the role of TNFIAP3 in the degradation of endocytic substances in nasal epithelial cells. The nasal epithelial cell line, RPMI 2650 cells (RPC), was cultured into monolayers in transwells. The endocytosis of staphylococcal enterotoxin B (SEB) by RPC monolayers was assessed by enzyme-linked immunoassay. The endocytosis of SEB-triggered endosome/lysosome fusion was observed by immunocytochemistry. The results showed that RPC monolayers expressed TNFIAP3 upon the endocytosis of SEB. Deficiency of TNFIAP3 resulted in abundant SEBs being transported to the basal chambers of transwells via the intracellular pathway. In the TNFIAP3-sufficient RPC, SEB-carrying endosomes fused with lysosomes were observed. The TNFIAP3-deficient RPC showed few SEB-carrying endosomes fused with lysosomes. In summary, TNFIAP3 plays an important role in tethering endosomes to lysosomes in RPC. Content Type Journal Article Pages 1-6 DOI 10.1007/s11010-012-1495-4 Authors Xingqiang Gao, Department of Otolaryngology & Head and Neck Surgery, First Hospital Affiliated Xiamen University, Xiamen, 361003 Fujian Province, China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Diabetes is associated with increased incidence of cardiovascular disease. Mechanisms that contribute to development of diabetic cardiopathy are not well understood. Phosphatidylinositol 3-kinase (PI3K) is a family of protein kinases that play an important role in regulation of cardiac function. It has been shown that inhibition of certain PI3K enzymes may produce cardiovascular protection. The aim of the present study was to determine whether chronic treatment with LY294002, an inhibitor of PI3K, can attenuate diabetes-induced cardiac dysfunction in isolated hearts obtained from normotensive and hypertensive rats. Recovery of cardiac function after 40 min of global ischemia and 30 min of reperfusion, measured as left ventricular developed pressure, left ventricular end-diastolic pressure, coronary flow and coronary vascular resistance, was worse in hearts obtained from diabetic and/or hypertensive animals compared to their respective controls. Treatment with LY294002 (1.2 mg/kg/day) for 4 weeks significantly prevented diabetes-induced cardiac dysfunction in both normotensive and hypertensive rats. Treatment with LY294002 did not significantly alter blood pressure or blood glucose levels. These results suggest that inhibition of PI3K signaling pathways can prevent ischemia/reperfusion-induced cardiac dysfunction in normotensive and hypertensive rats without correcting hyperglycemia or high blood pressure. Content Type Journal Article Pages 1-6 DOI 10.1007/s11010-012-1497-2 Authors Ibrahim F. Benter, Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait Ibrahim Al-Rashdan, Department of Medicine, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait Jasbir S. Juggi, Faculty of Allied Health, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait Mariam H. M. Yousif, Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait Saghir Akhtar, Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Recent studies have demonstrated that the Wnt/β-catenin signaling plays an important role in stem cell aging. However, the mechanisms of cell senescence induced by Wnt/β-catenin signaling are still poorly understood. Our preliminary study has indicated that activated Wnt/β-catenin signaling can induce MSC aging. In this study, we reported that the Wnt/β-catenin signaling was a potent activator of reactive oxygen species (ROS) generation in MSCs. After scavenging ROS with N -acetylcysteine, Wnt/β-catenin signaling-induced MSC aging was significantly attenuated and the DNA damage and the expression of p16 INK4A , p53, and p21 were reduced in MSCs. These results indicated that the Wnt/β-catenin signaling could induce MSC aging through promoting the intracellular production of ROS, and ROS may be the main mediators of MSC aging induced by excessive activation of Wnt/β-catenin signaling. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1498-1 Authors Da-yong Zhang, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Yu Pan, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Chong Zhang, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Bing-xi Yan, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Shan-shan Yu, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Dong-ling Wu, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Meng-meng Shi, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Kai Shi, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Xin-xiao Cai, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Shuang-shuang Zhou, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Jun-bo Wang, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Jian-ping Pan, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Li-huang Zhang, Department of Basic Medicine, School of Medicine and Life Sciences, Zhejiang University City College, 51 Huzhou Street, Hangzhou, 310015 People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Hyperglycemia is the major cause of diabetic angiopathy. Sarpogrelate hydrochloride is an antiplatelet drug, and expected to be useful in the treatment of chronic arterial occlusive diseases. The aim of our study was to evaluate the possible effects of sarpogrelate hydrochloride on adhesion molecule expression and its underlying mechanism in the prevention and treatment of cardiovascular disorders. Intercellular adhesion molecule-1 (ICAM-1) expression and superoxide dismutase (SOD) activity were determined after endothelial cells were exposed to high glucose in the absence and presence of sarpogrelate hydrochloride. Coincubation of endothelial cells with high glucose for 24 h resulted in a significant increase of monocyte–endothelial cell adhesion and the expression of ICAM-1 ( P  〈 0.01). These effects were abolished by sarpogrelate hydrochloride and sarpogrelate hydrochloride significantly increased SOD activities (40 ± 8 vs. 47 ± 7, n  = 8, P  〈 0.01). The low dose sarpogrelate group (0.1 μM) had significantly higher monocyte–endothelial cell adhesion and the expression of ICAM-1 than medium dose sarpogrelate group (1.0 μM) and high dose sarpogrelate group (10.0 μM) ( P  〈 0.05 for comparison among three groups and P  〈 0.01 for difference between low and high dose sarpogrelate groups). These findings suggested that sarpogrelate hydrochloride was able to protect vascular endothelium from dysfunction induced by high glucose. Content Type Journal Article Pages 1-5 DOI 10.1007/s11010-012-1490-9 Authors Ying Su, Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, NanGang, Harbin, 150001 China Nan Mao, Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, NanGang, Harbin, 150001 China Min Li, Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, NanGang, Harbin, 150001 China Xia Dong, Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, NanGang, Harbin, 150001 China Fan-Zhen Lin, Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, NanGang, Harbin, 150001 China Ying Xu, Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, NanGang, Harbin, 150001 China Yan-Bo Li, Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, NanGang, Harbin, 150001 China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    In addition of being an important inflammatory biomarker and a risk factor for cardiovascular disease, much evidence indicates that the C-reactive protein (CRP) contributes to the atherosclerosis development process. This plasmatic protein synthesized by hepatocytes in response to inflammation and tissue injury induces pro-inflammatory molecules' expression by endothelial cells (ECs). Previous studies showed that the 17β-estradiol (E2) has beneficial effects on vascular cells by reducing in vitro pro-inflammatory molecules expressions in EC. Therefore, we hypothesize that E2 blocks or reduces CRP-mediated inflammatory responses by modulating endogenous production of CRP in EC and/or activation mechanisms. Using human aortic ECs (HAECs), we first evaluated CRP production by vascular EC and second demonstrated its self-induction. Indeed, recombinant human CRP stimulation induces a fivefold increase of CRP expression. A 1-h pre-treatment of E2 at a physiologic dose (10 −9  M) leads to an important decrease of CRP production suggesting a partial blockage of its amplification loop mechanism. Furthermore, in HAEC, E2 reduces the secretion of the most potent agonist of CRP induction, the IL-6, by 21 %. E2 pre-treatment also decreased the expression of pro-inflammatory molecules IL-8, VCAM-1, and ICAM-1 induced by CRP and involved in leukocytes recruitment. In addition, we demonstrated that E2 could restore vascular endothelial growth factor-mediated EC migration response impaired by CRP suggesting another pro-angiogenic property of this hormone. These findings suggest that E2 can interfere with CRP pro-inflammatory effects via activation signals using its rapid, non-genomic pathway that may provide a new mechanism to improve vascular repair. Content Type Journal Article Pages 1-11 DOI 10.1007/s11010-012-1482-9 Authors Émilie Cossette, Research Center, Montreal Heart Institute, 5000 Bélanger Street, Montreal, QC H1T 1C8, Canada Isabelle Cloutier, Research Center, Montreal Heart Institute, 5000 Bélanger Street, Montreal, QC H1T 1C8, Canada Kim Tardif, Research Center, Montreal Heart Institute, 5000 Bélanger Street, Montreal, QC H1T 1C8, Canada Geneviève DonPierre, Research Center, Montreal Heart Institute, 5000 Bélanger Street, Montreal, QC H1T 1C8, Canada Jean-François Tanguay, Research Center, Montreal Heart Institute, 5000 Bélanger Street, Montreal, QC H1T 1C8, Canada Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Sarcoplasmic and t-tubule membrane proteins regulating sarcoplasmic Ca 2+ concentration exhibit fibre-type-dependent isoform expression, and play central roles in muscle contraction and relaxation. The purpose of this study was to evaluate the effects of in vitro electrical stimulation on the mRNA expression of components involved in Ca 2+ regulation in oxidative and glycolytic skeletal muscle. The mRNA level of Ca 2+ -ATPase (SERCA1, 2), calsequestrin (CASQ1, 2), ryanodine receptor (RyR1), and dihydropyridine receptor (Cacna1) was assessed in rat extensor digitorum longus (EDL) and soleus (SOL) muscles at 4 h of recovery following in vitro stimulations (either short intensive (SHO) 60 Hz, 5 min, or prolonged moderate (PRO) 20 Hz, 40 min). Stimulation induced acute regulation of the mRNA level of Ca 2+ -regulating proteins in a manner that does not follow typical fibre-type-specific transitions. In general, stimulation decreased mRNA content of all proteins studied. Most prominent down-regulation was observed for Cacna1 (26 and 32 % after SHO and PRO, respectively, in SOL; 19 % after SHO in EDL). SERCA1, SERCA2, CASQ1, CASQ2, and RyR1 mRNA content also decreased significantly in both muscles relative to resting control. Of notice is that hexokinase II mRNA content was increased in EDL and unchanged in SOL underlining the specificity of the down-regulation of mRNA of Ca 2+ regulatory proteins. The results demonstrate contraction-induced down-regulation of mRNAs for the main components of Ca 2+ -regulating system in skeletal muscle. The down-regulation of both isoforms of SERCA and CASQ after a single electrical stimulation session suggests that adaptations to repeated stimulation involve further regulatory mechanisms in addition to acute mRNA responses. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1486-5 Authors Satu Mänttäri, Department of Biology, University of Oulu, P.O. Box 3000, 90014 Oulu, Finland Niels Ørtenblad, Institute of Sports Science and Clinical Biomechanics, University of Southern Denmark, 5230 Odense, Denmark Klavs Madsen, Department of Public Health—Sport Science, University of Aarhus, 8000 Aarhus, Denmark Henriette Pilegaard, Department of Biology, Centre of Inflammation and Metabolism and Copenhagen Muscle Research Centre, University of Copenhagen, 2199 Copenhagen, Denmark Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    SASH1, a member of the SLY-family of signal adapter proteins, is a candidate tumor suppressor in breast and colon cancer. The SASH1 protein possesses both the SH3 and SAM domains, indicating that it may play an important role in intracellular signal transduction. Reduced expression of SASH1 is closely related to tumor growth, invasion, metastasis, and poor prognosis. However, the biological role of SASH1 remains unknown in osteosarcoma. To unravel the function of SASH1, we explored the expression of SASH1 in osteosarcoma tissues and its correlation to the clinical pathology of osteosarcoma and analyzed the relationship between SASH1 expression and cell cycle, apoptosis and invasion of osteosarcoma MG-63 cells, using the flow cytometry analysis and transwell invasion chamber experiments. Furthermore, the effect of SASH1 on the expression of cyclin D1, caspase-3, matrix metalloproteinase (MMP)-9 were observed by western blot. Our results showed that the expression rate of SASH1 mRNA in osteosarcoma tissues was significantly lower than that in normal bone tissue ( p  = 0.000), that the expression rate of SASH1 mRNA in the carcinoma tissues from patients with lung metastasis was significantly lower than that from patients without lung metastasis ( p  = 0.041), and that the expression rate of SASH1 mRNA also decreased with increasing Enneking stage ( p  = 0.032). However, the mRNA expression of SASH1 in osteosarcoma was independent of the patient’s gender, age, and tumor size ( p  = 0.983, 0.343, 0.517, respectively). The SASH1 protein displayed a down-regulation in osteosarcoma tissues compared to normal bone tissue ( p  = 0.000), displayed a down-regulation in osteosarcoma tissues from patients with lung metastasis compared to from patients without lung metastasis ( p  = 0.000), and displayed a gradual decrease with increasing Enneking stage ( p  = 0.000). In addition, the MG-63 cells from pcDNA3.1-SASH1 group exhibited significantly reduced cell viability, proliferation, and invasive ability compared to the empty vector group and blank control group ( p  = 0.023, 0.001, respectively), and there was no difference between the empty vector group and blank control group. The pcDNA3.1-SASH1 group displayed significantly more apoptotic cells than the empty vector group and blank control group ( p  = 0.004). The expression of cyclin D1, MMP-9 displayed a down-regulation in MG-63 cells from pcDNA3.1-SASH1 group compared to the empty vector group and blank control group ( p  = 0.000, 0.001, respectively) and the expression levels of caspase-3 displayed an up-regulation in MG-63 cells from pcDNA3.1-SASH1 group compared to the empty vector group and blank control group ( p  = 0.000). Taken together, these data indicated that the overexpression of SASH1 might be associated with the inhibition of growth, proliferation, and invasion of MG-63 cells and the promotion of apoptosis of MG-63 cells. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1491-8 Authors Qingbing Meng, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Minqian Zheng, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Hongbing Liu, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Changzhi Song, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Wensheng Zhang, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Juan Yan, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Ling Qin, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Xiaolan Liu, Orthopedics Department, Yancheng City No. 1 People’s Hospital, 16 Yue-He Road, Yancheng, 224005 Jiangsu, People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The present study was designed to evaluate the in vitro and in vivo ameliorative antioxidant potential of secoisolariciresinol diglucoside (SDG). In vitro antioxidant activity of synthetic SDG was carried out using DPPH, reducing power potency, and DNA protection assays. Wistar albino rats weighing 180–220 g were used for in vivo studies and liver damage was induced in the experimental animals by a single intraperitoneal (I.P.) injection of CCl 4 (2 g/kg b.w.). Intoxicated animals were treated orally with synthetic SDG at (12.5 and 25 mg/kg b.w.) and Silymarin (25 mg/kg) for 14 consecutive days. The levels of catalase (CAT), superoxide dismutase (SOD), peroxidase (POX), and lipid peroxidase (LPO) were measured in liver and kidney homogenates. The synthetic SDG exerts high in vitro antioxidant potency as it could scavenge DPPH at a IC 50 value of 78.9 μg/ml and has dose-dependent reducing power potency and protected DNA at 0.5 mg/ml concentration. Oral administration of synthetic SDG at 12.5 and 25 mg/kg b.w. showed significant protection compared to Silymarin (25 mg/kg) and the activities of CAT, SOD, and POX were markedly increased ( P  〈 0.05), whereas LPO significantly decreased ( P  〈 0.001) in a dose-dependent manner in liver and kidney in both pre- and post-treatment groups when compared to toxin-treated group. The results of in vitro and in vivo investigations revealed that synthetic SDG at 25 mg/kg b.w. is associated with beneficial changes in hepatic enzyme activities and thereby plays a key role in the prevention of oxidative damage in immunologic system. Content Type Journal Article Pages 1-9 DOI 10.1007/s11010-012-1487-4 Authors Sadiq S. Moree, Department of Biochemistry, Yuvaraja’s College, University of Mysore, Mysore, 570005 Karnataka, India J. Rajesha, Department of Biochemistry, Yuvaraja’s College, University of Mysore, Mysore, 570005 Karnataka, India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Marked sex-dependent differences in mitochondrial function and redox status have been found in brown adipose tissue (BAT) of control rats. Insulin also plays a role in the development and maintenance of this tissue. The aim was to investigate sexual dimorphism in the effects of diet-induced obesity on BAT mitochondrial function, as well as on insulin signaling pathway. 10-week-old Wistar rats of both sexes were fed a control diet or a palatable high-fat diet for 26 weeks. Serum markers of insulin sensitivity were analyzed. Mitochondrial DNA (mtDNA) content, mitochondrial oxidative activities, PGC-1α mRNA levels, as well as the protein levels of insulin receptor subunit β (IRβ), glucose transporter GLUT4, β 3 -adrenergic receptor (β 3 -AR), phosphatidylinositol 3-kinase, mitochondrial transcription factor A (TFAM), cytochrome c oxidase subunit IV (COX IV), and uncoupling protein 1 (UCP1) were measured in BAT. Obese females showed impaired systemic insulin sensitivity accompanied by diminished IRβ, GLUT4, and β 3 -AR protein levels in BAT. In addition, TFAM and COX IV protein and PGC-1α mRNA levels decreased in obese females, whereas mtDNA levels increased. In obese males, oxidative and thermogenic capacities rose and no significant changes were observed in the insulin signaling pathway elements. The reduction of the insulin signaling pathway in BAT of obese females may be responsible, at least partially, for the impaired biogenesis process, which could favor the increase of body weight found in this sex. In contrast, the enhanced mitochondrial functionality in the BAT of males would avoid increased oxidative damage and the impairment of insulin signaling. Content Type Journal Article Pages 1-11 DOI 10.1007/s11010-012-1481-x Authors A. Nadal-Casellas, Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Cra. Valldemossa km 7.5, 07122 Palma, Spain M. Bauzá-Thorbrügge, Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Cra. Valldemossa km 7.5, 07122 Palma, Spain A. M. Proenza, Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Cra. Valldemossa km 7.5, 07122 Palma, Spain M. Gianotti, Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Cra. Valldemossa km 7.5, 07122 Palma, Spain I. Lladó, Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Cra. Valldemossa km 7.5, 07122 Palma, Spain Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    To investigate the combined effect of aliskiren, a renin inhibitor, and AVE 0991, a Mas-receptor agonist, in experimental hypertension (HT) in rats. HT was produced by administration of deoxycorticosterone acetate (DOCA) and mean arterial blood pressure (MABP) was assessed by tail-cuff method. Treatments were started from 4th week onwards and were continued for 9 days. A significant increase in MABP was noted after 1 week in DOCA control rats, as compared with the base line value. A stable HT developed after 4 weeks of DOCA administration. Treatments with aliskiren and AVE 0991 alone, dose-dependently decreased MABP in DOCA-treated rats. Further, combination of low doses of aliskiren and AVE 0991 significantly reduced MABP, as compared with DOCA control rats and with either drug alone in low doses. It may be concluded that treatment with aliskiren produced down-regulation of both harmful Ang II–AT1-receptor and survival Ang(1–7)/Mas-receptor axis of RAAS. Treatment with combination of low doses of aliskiren and AVE 0991, for the first time, has been shown to produce synergistic blood pressure lowering effect. Therefore, combination of renin inhibitor with Mas-receptor agonist may prove beneficial for the treatment of hypertensive patients. Content Type Journal Article Pages 1-6 DOI 10.1007/s11010-012-1489-2 Authors Yogendra Singh, Department of Pharmacology, ISF College of Pharmacy, Moga, 142 001 Punjab, India Kulwinder Singh, University Institute of Pharmacy, Baba Farid University of Health Sciences, Faridkot, 151 203 Punjab, India P. L. Sharma, Department of Pharmacology, ISF College of Pharmacy, Moga, 142 001 Punjab, India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    An array of non-overgrown InAs/GaAs quantum dots has been decorated with adsorbed metal atoms in situ in ultrahigh vacuum. Their electron and photoemission properties have been studied. The radical modification of the spectra of the threshold emission from the quantum dots with increasing cesium coating has been found. Two photoemission channels have been established; they are characterized by considerably different intensities, spectral locations, and widths of the selective bands. It has been shown that the decoration of the quantum dots makes it possible to control the electronic structure and quantum yield of photoemission, the nature of which is related to the excitation of the electronic states of the GaAs substrate and InAs/GaAs quantum dots. Content Type Journal Article Category Condensed Matter Pages 332-335 DOI 10.1134/S0021364012170031 Authors G. V. Benemanskaya, Ioffe Physical Technical Institute, Russian Academy of Sciences, St. Petersburg, 194021 Russia M. N. Lapushkin, Ioffe Physical Technical Institute, Russian Academy of Sciences, St. Petersburg, 194021 Russia V. P. Evtikhiev, Ioffe Physical Technical Institute, Russian Academy of Sciences, St. Petersburg, 194021 Russia A. S. Shkol’nik, Ioffe Physical Technical Institute, Russian Academy of Sciences, St. Petersburg, 194021 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 5

Beschreibung:    Sulforaphane (SFN), a component of dietary cruciferous vegetables has been characterized for its anti-proliferative properties. We have recently demonstrated that pancreatic CSCs display activation of sonic hedgehog pathway which are fundamental drivers of stem cell renewal, and SFN inhibits the self-renewal of pancreatic CSCs in vitro. Consistent with these observations, we sought to determine the chemopreventive potential of SFN in an in vivo setting. We show here for the first time that sulforaphane treatment resulted in a significant reduction in the tumor growth of orthotopically implanted primary pancreatic CSCs isolated from human pancreatic tumors into the pancreas of NOD/SCID/IL2Rgamma mice, which is mediated through the modulation of Sonic hedgehog–GLI signaling. Hedgehog pathway blockade by SFN at a dose of 20 mg/kg resulted in a 45 % reduction in growth of pancreatic cancer tumors and reduced expression of Shh pathway components, Smo, Gli 1, and Gli 2 in mouse tissues. Further, SFN inhibited the expression of pluripotency maintaining transcription factors Nanog and Oct-4 and angiogenic markers VEGF and PDGFRα which are downstream targets of Gli transcription. Furthermore, SFN treatment resulted in a significant reduction in EMT markers Zeb-1, which correlated with increase in E-Cadherin expression suggesting the blockade of signaling involved in early metastasis. Interestingly, SFN downregulated the expression of Bcl-2 and XIAP to induce apoptosis. These data demonstrate that, at a tolerable dose, inhibition of Shh pathway by SFN results in marked reduction in EMT, metastatic, angiogenic markers with significant inhibition in tumor growth in mice. Since aberrant Shh signaling occurs in pancreatic tumorigenesis, therapeutics that target Shh pathway may improve the outcomes of patients with pancreatic cancer by targeting CSCs, thus suggesting the use of sulforaphane to further improve preventive and therapeutic approaches in patients with this devastating disease. Content Type Journal Article Pages 1-11 DOI 10.1007/s11010-012-1493-6 Authors Shih-Hui Li, Department of Pathology and Laboratory Medicine, The University of Kansas Cancer Center, The University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA Junsheng Fu, Department of Pathology and Laboratory Medicine, The University of Kansas Cancer Center, The University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA Dara Nall Watkins, Department of Pathology and Laboratory Medicine, The University of Kansas Cancer Center, The University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA Rakesh K. Srivastava, Department of Pharmacology, Toxicology and Therapeutics, and Medicine, The University of Kansas Cancer Center, The University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA Sharmila Shankar, Department of Pathology and Laboratory Medicine, The University of Kansas Cancer Center, The University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Dynamic remodeling of the actin cytoskeleton is crucial for biological processes such as cell migration and cell spreading. S100A10 is a member of the S100 protein family and is involved in intracellular trafficking and cell migration. In this study, we examined the role of S100A10 in actin cytoskeletal organization and cell spreading. Depletion of S100A10 induced disruption of stress fiber formation and delay in cell spreading. Rac1 activation during spreading was suppressed by S100A10 knockdown, and exogenous expression of active Rac1 restored the ability of cells to spread in the absence of S100A10. Our results demonstrate the crucial role of S100A10 in actin dynamics promoting cell spreading via Rac1 activation. Content Type Journal Article Pages 1-7 DOI 10.1007/s11010-012-1509-2 Authors Shurovi Sayeed, Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550 Japan Eri Asano, Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550 Japan Satoko Ito, Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550 Japan Kinji Ohno, Division of Neurogenetics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550 Japan Michinari Hamaguchi, Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550 Japan Takeshi Senga, Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550 Japan Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Chronic hepatitis B virus infection is the dominant global cause of hepatocellular carcinoma (HCC), especially hepatitis B virus-X (HBx) plays a major role in this process. HBx protein promotes cell cycle progression, inactivates negative growth regulators, and binds to and inhibits the expression of p53 tumor suppressor gene and other tumor suppressor genes and senescence-related factors. However, the relationship between HBx and autophagy during the HCC development is poorly known. Previous studies found that autophagy functions as a survival mechanism in liver cancer cells. We suggest that autophagy plays a possible role in the pathogenesis of HBx-induced HCC. The present study showed that HBx transfection brought about an increase in the formation of autophagosomes and autolysosomes. Microtubule-associated protein light chain 3, Beclin 1, and lysosome-associated membrane protein 2a were up-regulated after HBx transfection. HBx-induced increase in the autophagic level was increased by mTOR inhibitor rapamycin and was blocked by treatment with the PI3K–Akt inhibitor LY294002. The same results can also be found in HepG2.2.15 cells. These results suggest that HBx activates the autophagic lysosome pathway in HepG-2 cells through the PI3K–Akt–mTOR pathway. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1457-x Authors Peng Wang, Department of Hepatobiliary Surgery, First Affiliated Hospital of Soochow University, Suzhou, 215004 People’s Republic of China Qing-song Guo, Department of Hepatobiliary Surgery, Affiliated Hospital of Nantong University, Nantong, 226001 People’s Republic of China Zhi-wei Wang, Department of Hepatobiliary Surgery, Affiliated Hospital of Nantong University, Nantong, 226001 People’s Republic of China Hai-xin Qian, Department of Hepatobiliary Surgery, First Affiliated Hospital of Soochow University, Suzhou, 215004 People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Emerging evidences demonstrate that excess aldosterone and insulin interact at target tissues. It has been shown that increased levels of aldosterone contribute to the development of insulin resistance and thus act as a risk factor for the development of type-2 diabetes mellitus. However, the molecular mechanisms involved in this scenario are yet to be identified. This study was designed to assess the dose-dependent effects of aldosterone on insulin signal transduction and glucose oxidation in the skeletal muscle (gastrocnemius) of adult male rat. Healthy adult male albino rats of Wistar strain ( Rattus norvegicus ) weighing 180–200 g were used in this study. Rats were divided into four groups. Group I: control (treated with 1 % ethanol only), group II: aldosterone treated (10 μg /kg body weight, twice daily for 15 days), group III: aldosterone treated (20 μg /kg body weight, twice daily for 15 days), and group IV: aldosterone treated (40 μg/kg body weight, twice daily for 15 days). Excess aldosterone caused glucose intolerance in a dose-dependent manner. Serum insulin and aldosterone were significantly increased, whereas serum testosterone was decreased. Aldosterone treatment impaired the rate of glucose uptake, oxidation, and insulin signal transduction in the gastrocnemius muscle through defective expression of IR, IRS-1, Akt, AS160, and GLUT4 genes. Phosphorylation of IRS-1, β-arrestin-2, and Akt was also reduced in a dose-dependent manner. Excess aldosterone results in glucose intolerance as a result of impaired insulin signal transduction leading to decreased glucose uptake and oxidation in skeletal muscle. In addition to this, it is inferred that excess aldosterone may act as one of the causative factors for the onset of insulin resistance and thus increased incidence of type-2 diabetes. Content Type Journal Article Pages 1-14 DOI 10.1007/s11010-012-1452-2 Authors Jayaraman Selvaraj, Department of Endocrinology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai, 600113 India Sampath Sathish, Department of Endocrinology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai, 600113 India Chinnaiyan Mayilvanan, Department of Endocrinology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai, 600113 India Karundevi Balasubramanian, Department of Endocrinology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar Campus, Taramani, Chennai, 600113 India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The mass spectrometry study has indicated that the magnetic field accelerates the oxidation of the surface of silicon crystals. The oxidation rate also depends on the nuclear spin of silicon: the oxidation rate of atoms with magnetic nuclei ( 29 Si) is almost twice as high as that of atoms with spinless, unmagnetized nuclei ( 28 Si and 30 Si). Both effects—magnetic field and magnetic isotope—reliably prove that the oxidation of silicon is a spin-selective reaction involving radicals and radical pairs as intermediate paramagnetic particles. A spin-selective magnetic sensitive oxidation mechanism is discussed. Content Type Journal Article Category Condensed Matter Pages 102-104 DOI 10.1134/S002136401214007X Authors O. V. Koplak, Scientific-Educational Center FKhM, Kyiv University and National Academy of Sciences of Ukraine, Kyiv, 01033 Ukraine R. B. Morgunov, Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia A. L. Buchachenko, Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    An explanation of an anomalously narrow microwave absorption line in superfluid 4 He has been proposed. It has been shown that the experimentally observed resonance linewidth agrees with the assumption of parametric excitation of a macroscopic coherent roton state. Content Type Journal Article Category Condensed Matter Pages 98-98 DOI 10.1134/S0021364012140081 Authors L. A. Melnikovsky, Institute for Physical Problems, Russian Academy of Sciences, ul. Kosygina 2, Moscow, 119334 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    A light emitting diode has been developed on the basis of multilayer nanostructures in which CdSe/CdS semiconductor colloidal quantum dots serve as emitters. Their absorption, photo-, and electroluminescence spectra have been obtained. The strong influence of the size effect and the density of particles in the layer on the spectral and electrophysical characteristics of the diode has been demonstrated. It has been shown that the rates of the transfer of the exciton excitation energy from organic molecules to quantum dots increase strongly even at a small increase in the radius of the core (CdSe) of a particle and depend strongly on the thickness of the shell (CdS) of the particle. The optimal arrangement of the layer of quantum dots with respect to the p-n junction has been estimated from the experimental data. The results demonstrate that the spectral characteristics and rates of the electron processes in light-emitting devices based on quantum dots incorporated into an organic matrix can be efficiently controlled. Content Type Journal Article Category Condensed Matter Pages 113-117 DOI 10.1134/S0021364012140135 Authors A. A. Vashchenko, Lebedev Physical Institute, Russian Academy of Sciences, Moscow, 119991 Russia V. S. Lebedev, Lebedev Physical Institute, Russian Academy of Sciences, Moscow, 119991 Russia A. G. Vitukhnovskii, Lebedev Physical Institute, Russian Academy of Sciences, Moscow, 119991 Russia R. B. Vasiliev, Faculty of Materials Science, Moscow State University, Moscow, 119992 Russia I. G. Samatov, Faculty of Materials Science, Moscow State University, Moscow, 119992 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    The polarization properties of extraordinary microwave transmission through perforated duralumin films are investigated both theoretically and experimentally. It is shown that resonance wavelength at which transmission efficiency reaches maximum value depends on the incident radiation polarization. Content Type Journal Article Category Condensed Matter Pages 99-101 DOI 10.1134/S0021364012140068 Authors S. E. Grigas, Faculty of Physics, Moscow State University, Moscow, 119992 Russia A. G. Rzhanov, Faculty of Physics, Moscow State University, Moscow, 119992 Russia V. N. Semenenko, Institute for Theoretical and Applied Electrodynamics, Russian Academy of Sciences, Moscow, 125412 Russia V. A. Chistyaev, Institute for Theoretical and Applied Electrodynamics, Russian Academy of Sciences, Moscow, 125412 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    We investigate the disturbance of the InAs nanowire resistance by a conductive tip of a scanning probe micro-scope at helium temperature as a function of the tip position in close vicinity to the nanowire. At the tip displacement along the wire the resistance ( R wire ∼ 30 kΩ, what is typical for diffusive regime) demonstrates quasi-periodical oscillations with an amplitude about 3%. The period of the oscillations depends on the number of electrons in the nanowire and is consistent with expected for standing electron waves caused by ballistic electrons in the top subband of the InAs nanowire. Content Type Journal Article Category Condensed Matter Pages 109-112 DOI 10.1134/S0021364012140159 Authors A. A. Zhukov, Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia Ch. Volk, Grünberg Institut (PGI-9), Forschungszentrum Jülich, 52425 Jülich, Germany A. Winden, Grünberg Institut (PGI-9), Forschungszentrum Jülich, 52425 Jülich, Germany H. Hardtdegen, Grünberg Institut (PGI-9), Forschungszentrum Jülich, 52425 Jülich, Germany Th. Schäpers, Grünberg Institut (PGI-9), Forschungszentrum Jülich, 52425 Jülich, Germany Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    Schramm-Loewner evolution (SLE) and conformal field theory (CFT) are popular and widely used instruments to study critical behavior of two-dimensional models, but they use different objects. While SLE has natural connection with lattice models and is suitable for strict proofs, it lacks computational and predictive power of conformal field theory. To provide a way for the concurrent use of SLE and CFT, CFT correlation functions, which are martingales with respect to SLE, are considered. A relation between parameters of Schramm-Loewner evolution on coset space and algebraic data of coset conformal field theory is revealed. The consistency of this approach with the behavior of parafermionic and minimal models is tested. Coset models are connected with off-critical massive field theories and implications of SLE are discussed. Content Type Journal Article Category Fields, Particles, and Nuclei Pages 90-93 DOI 10.1134/S0021364012140093 Authors A. Nazarov, Department of High-Energy and Elementary Particle Physics, Faculty of Physics and Chebyshev Laboratory, Faculty of Mathematics and Mechanics, St. Petersburg State University, St. Petersburg, 198904 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    Hadron production in lepton-nucleus interactions at high energies is considered in the framework of developing Monte Carlo event generator HARDPING (HARD Probe INteraction Generator). Such effects as formation length, energy loss and multiple rescattering for produced hadrons and their constituents are implemented in the HARDPING 2.0 generator. Available data from HERMES collaboration on hadron production in lepton-nucleus collisions are described by the present version of the HARDPING generator in a reasonable agreement. Content Type Journal Article Category Fields, Particles, and Nuclei Pages 85-89 DOI 10.1134/S0021364012140020 Authors Ya. A. Berdnikov, St.-Petersburg State Polytechnical University, St. Petersburg, 195251 Russia A. E. Ivanov, St.-Petersburg State Polytechnical University, St. Petersburg, 195251 Russia V. T. Kim, St.-Petersburg State Polytechnical University, St. Petersburg, 195251 Russia V. A. Murzin, St. Petersburg Nuclear Physics Institute, Gatchina, 188300 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    A simple description has been proposed for the renormalization of the conduction band parameters in cuprates owing to the interaction of the current carriers with phonons. Kinks in the quasiparticle dispersion law in the optical phonon mode region (70 meV, compound Bi 2 Sr 2 CaCu 2 O 8 − x ) and data on the temperature dependence of the superconducting current density in YBa 2 Cu 3 O 7 have been analyzed. Ideas of new experiments have been discussed. Content Type Journal Article Category Condensed Matter Pages 105-108 DOI 10.1134/S0021364012140044 Authors M. V. Eremin, Institute of Physics, Kazan (Volga Region) Federal University, Kazan, 420008 Russia M. A. Malakhov, Institute of Physics, Kazan (Volga Region) Federal University, Kazan, 420008 Russia D. A. Sunyaev, Institute of Physics, Kazan (Volga Region) Federal University, Kazan, 420008 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    Spectral properties of LiFeAs superconductor are investigated within the LDA+DMFT method. Calculated distribution of the spectral weight in the k -space is in good agreement with angle-resolved photoemission (ARPES) spectra. Calculated effective electron mass enhancement factor m */ m ≈ 3 is close to the one estimated from comparison of density-functional theory results with ARPES spectra. Our results demonstrate that inclusion into consideration of dynamical Coulomb correlations between the electrons plays a key role in understanding of the spectral properties of LiFeAs. Content Type Journal Article Category Condensed Matter Pages 118-122 DOI 10.1134/S0021364012140111 Authors S. L. Skornyakov, Institute of Metal Physics, Ural Branch, Russian Academy of Sciences, Yekaterinburg, 620990 Russia D. Y. Novoselov, Institute of Metal Physics, Ural Branch, Russian Academy of Sciences, Yekaterinburg, 620990 Russia T. Gürel, Department of Physics, Namik Kemal University, 59030 Tekirdag, Turkey V. I. Anisimov, Institute of Metal Physics, Ural Branch, Russian Academy of Sciences, Yekaterinburg, 620990 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    We predict the insulator-metal-insulator transitions for the temperature and pressure of the lower mantle with the metal layer thickness Δ h ≈ 400 km at the depth of 1400–1800 km. The insulator-metal transition has the Mott-Hubbard origin, while the second transition from metal to insulator results from spin crossover of the Fe 2+ ions from high spin S = 2 to low spin S = 0 state. The conductivity in the metal layer may attain 250 S/m. The depth profile of the conductivity is also suggested. Content Type Journal Article Category Miscellaneous Pages 129-132 DOI 10.1134/S002136401214010X Authors S. G. Ovchinnikov, Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia T. M. Ovchinnikova, Sukhachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia P. G. Dyad’kov, Trofimuk Institute of Petroleum-Gas Geology and Geophysics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia V. V. Plotkin, Trofimuk Institute of Petroleum-Gas Geology and Geophysics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia K. D. Litasov, Sobolev Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    Experimental studies of the phase diagram of Bose condensation in a system of spatially indirect dipolar excitons in GaAs/AlGaAs quantum wells are reviewed. The properties of spatially periodic patterns arising in the luminescence of the exciton Bose condensate in a ring-shaped potential trap and the coherence of the condensate luminescence are discussed. Content Type Journal Article Category Scientific Summaries Pages 138-147 DOI 10.1134/S0021364012140056 Authors A. V. Gorbunov, Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia V. B. Timofeev, Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    Photoemission induced by vacuum ultraviolet resonance radiation of xenon atoms from the surface of a solid in vacuum and in the case of a target in contact with a gas has been experimentally studied. It has been demonstrated that the photoemission response increases strongly (up to an order of magnitude) under the adsorption (or implantation) of gas atoms into the target when vacuum ultraviolet radiation resonantly acts on these atoms. This is due to different mechanisms of photoemission from the surface of the solid in vacuum and from the surface in contact with the gas. The notion of activated resonant photoemission has been introduced. Content Type Journal Article Category Miscellaneous Pages 133-137 DOI 10.1134/S0021364012140032 Authors P. A. Bokhan, Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, pr. akademika Lavrent’eva 13, Novosibirsk, 630090 Russia D. E. Zakrevsky, Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, pr. akademika Lavrent’eva 13, Novosibirsk, 630090 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    The nature of the set of free fields that represent the system at the critical point has been revealed by studying the correlation functions of the degrees of freedom of the gauge supersymmetric Ising model on the cubic lattice. The same set of free fields represents the continuous supersymmetric Abelian gauge theory. Thus, the name of the lattice system is appropriate. Comparison with the two-dimensional Ising model is given. Content Type Journal Article Category Methods of Theoretical Physics Pages 123-128 DOI 10.1134/S0021364012140147 Authors S. N. Vergeles, Landau Institute for Theoretical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    The possibility of the generation of quasi-cw terahertz radiation by the optical rectification method for broad-band Fourier unlimited nanosecond laser pulses has been experimentally demonstrated. The broadband radiation of a LiF dye-center laser is used as a pump source of a nonlinear optical oscillator. The energy efficiency of terahertz optical frequency conversion in a periodically polarized lithium niobate crystal is 4 × 10 −9 at a pump power density of 7 MW/cm 2 . Content Type Journal Article Category Astrophysics and Cosmology Pages 94-97 DOI 10.1134/S0021364012140123 Authors A. N. Tuchak, Moscow State University, Moscow, 119991 Russia G. N. Gol’tsman, Moscow State Pedagogical University, ul. Malaya Pirogovskaya 1, Moscow, 119991 Russia G. Kh. Kitaeva, Moscow State University, Moscow, 119991 Russia A. N. Penin, Moscow State University, Moscow, 119991 Russia S. V. Seliverstov, Moscow State Pedagogical University, ul. Malaya Pirogovskaya 1, Moscow, 119991 Russia M. I. Finkel, Moscow State Pedagogical University, ul. Malaya Pirogovskaya 1, Moscow, 119991 Russia A. V. Shepelev, Gubkin State University of Oil and Gas, Leninskii pr. 65, Moscow, 119991 Russia P. V. Yakunin, Moscow State University, Moscow, 119991 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 2

Beschreibung:    Activation of protein kinase C (PKC) is a critical intracellular signaling triggered by ischemic preconditioning (IPC), but the precise mechanisms underlying the actions of PKC in IPC-mediated cardioprotection remain unclear. Here, we investigated the role of PKC activation on the antioxidant activity by IPC in rabbit hearts. Isolated rabbit hearts were subjected to 60 min of global ischemia by cold cardioplegic arrest (4 °C) and 60 min of reperfusion (37 °C). IPC was induced by three cycles of 2-min ischemia following 3 min of reperfusion (37 °C) before cardioplegic arrest. IPC resulted in a better recovery of mechanical function, increased tissue reduced glutathione-to-oxidized glutathione ratio (GSH/GSSG), superoxide dismutase and catalase content, and decreased tissue malondialdehyde (MDA) content compared to control hearts subjected to 60 min of cardioplegic ischemia and 60 min of reperfusion. IPC also significantly induced activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the inductions of antioxidant genes heme oxygenase-1 (HO-1) and manganese superoxide dismutase (MnSOD). Injection of phorbol 12-myristate 13 acetate, an activator of PKC, before cardioplegic ischemia induced translocation of PKC-δ and -ε isoforms to membrane fraction, nuclear accumulation of Nrf2, and conferred cardioprotection similar to IPC. Polymyxin B, an inhibitor of PKC, blocked the membrane translocation of PKC-δ and -ε during IPC, inhibited Nrf2 nuclear accumulation, and significantly diminished the IPC-induced cardioprotection when administrated before IPC. These results indicate that the activation of PKC induces the translocation of Nrf2 and the enhancement of endogenous antioxidant defenses in the IPC hearts and suggest that PKC may target Nrf2 to confer cardioprotection. Content Type Journal Article Pages 1-11 DOI 10.1007/s11010-012-1458-9 Authors Xin Zhang, Department of Cardiac Surgery, Zhengzhou University College of Medicine, Zhengzhou, 450052 China Zhibin Xiao, Department of Cardiac Surgery, Beijing General Hospital of PLA, Beijing, 100700 China Jianmin Yao, Department of Cardiac Surgery, Beijing General Hospital of PLA, Beijing, 100700 China Genshang Zhao, Department of Cardiac Surgery, Zhengzhou University College of Medicine, Zhengzhou, 450052 China Xianen Fa, Department of Cardiac Surgery, Zhengzhou University College of Medicine, Zhengzhou, 450052 China Jianli Niu, Department of Cardiac Surgery, Beijing General Hospital of PLA, Beijing, 100700 China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Serotonin (5-HT) is a hormone that has been implicated in the regulation of many physiological and pathological events. One of the most intriguing properties of this hormone is its ability to up-regulate mitosis. Moreover, 5-HT stimulates glucose uptake and up-regulates PFK activity through the 5-HT 2A receptor, resulting in the phosphorylation of a tyrosine residue of PFK and the intracellular redistribution of PFK within skeletal muscle. The present study investigated some of the signaling intermediates involved in the effects of 5-HT on 6-phosphofructo-1-kinase (PFK) regulation from skeletal muscle using kinetic assessments, immunoprecipitation, and western blotting assays. Our results demonstrate that 5-HT stimulates PFK from skeletal muscle via phospholipase C (PLC). The activation of PLC in skeletal muscle leads to the recruitment of protein kinase C (PKC) and calmodulin and the stimulation of calmodulin kinase II, which associates with PFK upon 5-HT action. Alternatively, 5-HT loses its ability to up-regulate PFK activity when Janus kinase is inhibited, suggesting that 5-HT is able to control glycolytic flux in the skeletal muscle of mice by recruiting different pathways and controlling PFK activity. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1462-0 Authors Wagner Santos Coelho, Laboratório de Enzimologia e Controle do Metabolismo (LabECoM), Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ 21941-590, Brazil Mauro Sola-Penna, Laboratório de Enzimologia e Controle do Metabolismo (LabECoM), Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ 21941-590, Brazil Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Upon release from keratinocytes, 14-3-3 sigma (also known as stratifin) acts on the dermal fibroblast and modulates its production of extracellular matrix proteins. Subsequent to the recent identification as a receptor responsible for stratifin-mediated matrix turnover in dermal fibroblasts, aminopeptidase N has been implicated in the regulation of epidermal–dermal communication and expression of key matrix proteases and adhesion molecules. In light of the growing importance of aminopeptidase N in modulation of the fibroblast phenotype, the present study evaluates the potential of targeting the ectoenzyme in cutaneous repair, and demonstrates that neutralization of aminopeptidase N led to acceleration of wound closure. This was attributed to at least in part an increase of collagen deposition and fibroblast contractility in the granulation tissue. These findings confirmed the important role of aminopeptidase N in post-injury tissue remodeling and wound contraction. Content Type Journal Article Pages 1-6 DOI 10.1007/s11010-012-1449-x Authors Amy Lai, Burn and Wound Healing Research Laboratory, ICORD, the Blusson Spinal Cord Centre, Department of Surgery, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada Azadeh Hosseini-Tabatabaei, Burn and Wound Healing Research Laboratory, ICORD, the Blusson Spinal Cord Centre, Department of Surgery, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada Ryan Hartwell, Burn and Wound Healing Research Laboratory, ICORD, the Blusson Spinal Cord Centre, Department of Surgery, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada Elham Rahmani-Neishaboor, Burn and Wound Healing Research Laboratory, ICORD, the Blusson Spinal Cord Centre, Department of Surgery, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada Ruhangiz Taghi Kilani, Burn and Wound Healing Research Laboratory, ICORD, the Blusson Spinal Cord Centre, Department of Surgery, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada Aziz Ghahary, Burn and Wound Healing Research Laboratory, ICORD, the Blusson Spinal Cord Centre, Department of Surgery, University of British Columbia, 818 West 10th Avenue, Vancouver, BC V5Z 1M9, Canada Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The ethylene glycol-1,4-dioxane system is studied by means of differential scanning calorimetry over a wide range of temperatures (−90 to 25°C) and is found to be a simple eutectic with the eutectic point at 10 mol % of dioxane (−16.5°C). Unlike a water-dioxane system, in which the clathrate with dioxane: H 2 O = 1: 34 ratio is formed, the observed phase diagram showed no evidence of clathrate formation, due presumably to its hydrogen bond geometry and the intermolecular interaction properties of ethylene glycol. Content Type Journal Article Category Short Communications Pages 1745-1746 DOI 10.1134/S0036024412110234 Authors M. N. Rodnikova, Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, 119991 Russia I. A. Solonina, Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, 119991 Russia M. R. Kiselev, Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, 119071 Russia S. V. Makaev, Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, 119991 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    The solubility of atmospheric oxygen in solutions of surfactants of different natures at 293 K and pH 8 is determined by gas chromatography. It is found that additives of nonionic surfactants decrease the oxygen content in the solution in the premicellar region and increase its solubility in the micellar region. It is shown that, for anionic surfactants, a decrease in the solubility of O 2 is observed over the entire concentration range. Content Type Journal Article Category Short Communications Pages 1753-1755 DOI 10.1134/S0036024412110088 Authors G. V. Chistyakova, Krestov Institute of Solution Chemistry, Russian Academy of Sciences, Ivanovo, 153045 Russia S. A. Koksharov, Krestov Institute of Solution Chemistry, Russian Academy of Sciences, Ivanovo, 153045 Russia T. V. Vladimirova, Ivenergo, Ivanovo, 153326 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    It was shown that after partial dehydration occurs a simultaneous condensation of four mol of initial monomer Gd(NO 3 ) 3 · 6H 2 O into a tetramer Gd 4 O 4 (NO 3 ) 4 . The heterocycle containing 4 gadolinium atoms gradually loses N 2 O 5 and, through the formation of unstable oxynitrates, is transformed into Gd 2 O 3 . The interatomic distances and angles were calculated using the molecular mechanics method. The comparison of the potential energies of consecutive oxyphosphates permitted an evaluation of their stability. The models of intermediate oxynitrates represent a reasonably good approximation to the real structures and a proper interpretation of experimental data. Content Type Journal Article Category Chemical Kinetics and Catalysis Pages 1659-1663 DOI 10.1134/S0036024412110180 Authors P. P. Melnikov, Federal University of Mato Grosso do Sul/UFMS, Caixa Postal 549, Campo Grande/MS, Brazil V. A. Nascimento, Federal University of Mato Grosso do Sul/UFMS, Caixa Postal 549, Campo Grande/MS, Brazil L. Z. Zanoni Consolo, Federal University of Mato Grosso do Sul/UFMS, Caixa Postal 549, Campo Grande/MS, Brazil Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    A dimensionless complex containing the surface free energy of a crystal-liquid interface γ, and the entropy jump, temperature, and density of a crystal phase is described using the phenomenology of thermodynamic similarity; this complex remains constant at the melting line. It is demonstrated that the complex refines the result obtained by Skripov and Faizullin in [6] and enables us to estimate the temperature dependence of γ. Our calculations show that the surface free energy of the crystal-liquid interface of normally melting compounds is a monotonically increasing function of temperature. Content Type Journal Article Category Short Communications Pages 1763-1765 DOI 10.1134/S0036024412110040 Authors V. G. Baidakov, Institute of Thermal Physics, Ural Branch, Russian Academy of Sciences, Yekaterinburg, 620016 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    Thermodynamic parameters are determined for the adsorption of vapors of hydrocarbons and polar compounds of different structure on carbon adsorbent modified by a monomolecular layer of heptakis (2,3,6-tri- O -benzoyl)-β-cyclodextrin. The effect of the structure and polarity of organic compounds on adsorption onto an adsorbent support with a chiral macrocyclic modifier are considered. Content Type Journal Article Category Short Communications Pages 1769-1772 DOI 10.1134/S0036024412110155 Authors K. A. Kopytin, Samara State University, Samara, 443011 Russia S. Yu. Kudryashov, Samara State University, Samara, 443011 Russia N. G. Gerasimova, Samara State University, Samara, 443011 Russia L. A. Onuchak, Samara State University, Samara, 443011 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    A thermodynamic variation of the Lindemann criterion for the vaporization of metals is proposed. It is shown that the critical amplitude of vibrations of atoms at the boiling point averages 1.42 bond lengths. Close values of interatomic distances result from the Vinet universal equation for the atomization of metals under the action of high temperatures (1.48) and negative pressures (1.50). The last value corresponds to the Van der Waals distances between metal atoms. Content Type Journal Article Category Short Communications Pages 1759-1762 DOI 10.1134/S0036024412110052 Authors S. S. Batsanov, Institute of Structural Macrokinetics and Problems of Materials Science, Russian Academy of Sciences, Chernogolovka, Moscow oblast, 142432 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    Samples of composite nanomaterials obtained by the thermal treatment of mixtures of MoO 3 nano-dispersed powder and ultrafine powder of Mo with precipitate from removing iron from groundwater are studied by means of X-ray diffraction and infrared spectroscopy. The structure of these samples (phase composition, average crystallite size, microdistortions (microstresses) of their crystal lattices, and certain texture parameters) are determined. It is suggested that under certain conditions, shells from the nanoparticles of Mo and/or MoO 3 are formed on the surface of sediment particles, preventing the identification of iron-containing phases. Estimates are made of the sorption activity of some materials with respect to carbon monoxide (CO). Content Type Journal Article Category Physical Chemistry of Nanoclusters and Nanomaterials Pages 1689-1696 DOI 10.1134/S0036024412110210 Authors L. Yu. Novoselova, Institute of Petroleum Chemistry, Siberian Branch, Russian Academy of Sciences, Tomsk, 634021 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    A table for determining the first derivatives of thermodynamic parameters is proposed. The table differs from the familiar Suvorov table in that the five dimension parameters are replaced with four dimensionless thermodynamic parameters. Content Type Journal Article Category Short Communications Pages 1747-1750 DOI 10.1134/S003602441211009X Authors V. P. Dobrodeev, Rybinsk State Aviation Technological Academy, Rybinsk, 152934 Russia N. A. Kalyaeva, Rybinsk State Aviation Technological Academy, Rybinsk, 152934 Russia A. V. Dobrodeev, Rybinsk State Aviation Technological Academy, Rybinsk, 152934 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    The conjugated diffusion transport of amino acid and mineral salt through a profiled sulfo group cation exchange membrane that simulates the extraction of amino acid from wash waters of microbiological production containing mineral components not used in synthesis is studied. The competitive nature of the conjugation of flows resulting in a decrease in the rate of the mass transfer of components and their separation factor is established from a comparative analysis of experimental data on the diffusion transfer of phenylalanine and sodium chloride in the form of hydrogen from individual and mixed solutions through a profiled sulfo group cation exchange membrane. The range of concentrations and the ratio of components in solution corresponding to the effective separation of phenylalanine and sodium chloride are determined. Content Type Journal Article Category Physical Chemistry of Separation Processes: Chromatography Pages 1726-1731 DOI 10.1134/S0036024412110271 Authors V. I. Vasil’eva, Voronezh State University, Voronezh, 394006 Russia E. A. Vorob’eva, Voronezh State University, Voronezh, 394006 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 11

Beschreibung:    NF-κB consists of p50, p65 (RelA), p52, c-Rel, and RelB, and among them p65 is a representative protein to investigate the regulation and function of this signaling. NF-κB integrates inflammation and carcinogenesis and regulates the expression of a variety of genes in response to immunity, inflammation, and apoptosis. IκBα acts as an inhibitor of NF-κB through forming an inactive NF-κB/IκBα complex. Pokemon is a ubiquitous transcription factor involved in different signaling pathways, playing a pivotal role in cell proliferation, anti-apoptosis, embryonic development, and maintenance. In this study, we found that p65 and IκBα are both novel regulatory targets of Pokemon. Ectopic expression of Pokemon in immortalized liver cells HL7702 enhanced p65 and IκBα expression, whereas silencing of Pokemon in hepatocellular carcinoma cells QGY7703 reduced cellular p65 levels. ChIP assay and targeted mutagenesis revealed that Pokemon directly binds to the element of −434 to −430 bp in p65 promoter and of −453 to −448 bp in IκBα promoter and stimulates luciferase reporter gene expression. Co-transfection of Pokemon with p65 or IκBα promoter-reporter notably enhanced their promoter activity. These data suggest that Pokemon activates the expression of both p65 and IκBα by sequence-specific binding to their promoters and plays a dual role in regulating NF-κB signaling. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1445-1 Authors Nan-Nan Zhang, Department of Chemistry, Tsinghua University, Beijing, 100084 People’s Republic of China Qin-Sheng Sun, The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055 People’s Republic of China Zhe Chen, Department of Chemistry, Tsinghua University, Beijing, 100084 People’s Republic of China Feng Liu, The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055 People’s Republic of China Yu-Yang Jiang, Department of Chemistry, Tsinghua University, Beijing, 100084 People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    We describe the development and characterization of a new cell line, designated Xl 1, derived from vertebra and long bones of Xenopus laevis . These cells can mineralize their extracellular matrix upon addition of an inorganic phosphate donor and vitamin C, as characterized by von Kossa staining. In addition they express genes such as matrix gla protein ( mgp ), alkaline phosphatase , type II collagen , and retinoic acid receptors , representing a valuable tool to analyze expression and regulation of Xenopus cartilage-associated genes. Continuous treatment with retinoic acid (RA) inhibited mineralization, alkaline phosphatase expression and its activity, suggesting that RA is a potential negative regulator of Xl 1 cell differentiation. These cells are receptive to efficient transfer of DNA using conventional methods including calcium phosphate, liposome-mediated transfer or electroporation and were found to express basal levels of mgp at least 50-fold higher than the routinely used Xenopus A6 cell line, as seen by transcription assays with the distal X. laevis mgp promoter. Being the first amphibian cell line derived from bone tissue, the Xl 1 culture provides an excellent in vitro tool for functional promoter studies, being suitable, among other uses, for identifying promoter elements mediating cartilage-expressed genes as shown here for mgp . Content Type Journal Article Pages 1-11 DOI 10.1007/s11010-012-1473-x Authors Natércia Conceição, Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, Portugal Michael Viegas, Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, Portugal João Fidalgo, Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, Portugal M. Leonor Cancela, Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, Portugal Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Insulin-like growth factor (IGF)-I and IGF-II play major roles in the regulation of skeletal muscle growth and differentiation, and both are locally expressed in muscle cells. Recent studies have demonstrated that IGF-II up-regulates its own gene expression during myogenesis and this auto-regulatory loop is critical for muscle differentiation. How local IGF-I is regulated in this process is unclear. Here, we report that while IGF-II up-regulated its own gene expression, it suppressed IGF-I gene expression during myogenesis. These opposite effects of IGF-II on IGF-I and IGF-II genes expression were time dependent and dose dependent. It has been shown that IGFs activate the PI3K-Akt-mTOR, p38 MAPK, and Erk1/2 MAPK pathways. In myoblasts, we examined their role(s) in mediating the opposite effects of IGF-II. Our results showed that both the PI3K-Akt-mTOR and p38 MAPK pathways played critical roles in increasing IGF-II mRNA expression. In contrast, mTOR was required for down-regulating the IGF-I gene expression by IGF-II. In addition, Akt, Erk1/2 MAPK, and p38 MAPK pathways were also involved in the regulation of basal levels of IGF-I and IGF-II genes during myogenesis. These findings reveal a previously unrecognized negative feedback mechanism and extend our knowledge of IGF-I and IGF-II gene expression and regulation during myogenesis. Content Type Journal Article Pages 1-7 DOI 10.1007/s11010-012-1479-4 Authors Shuang Jiao, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003 People’s Republic of China Hongxia Ren, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA Yun Li, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003 People’s Republic of China Jianfeng Zhou, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003 People’s Republic of China Cunming Duan, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA Ling Lu, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003 People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    To know the involvement of glycosaminoglycans (GAGs) in the metastasis of mouse FBJ osteosarcoma cells, N α -lauroyl- O -(β- d -xylopyranosyl)- l -serinamide (Xyl-Ser-C12), which initiates elongation of GAG chains using the glycan biosynthesis system in cells, was administered to FBJ cells with different metastatic capacities. Production of glycosylated products derived from Xyl-Ser-C12, especially heparan sulfate (HS) GAG-type oligosaccharides such as GalNAc-GlcA-GlcNAc-GlcA-Gal-Gal-Xyl-Ser-C12, was indicated in poorly metastatic FBJ-S1 cells more than in highly metastatic FBJ-LL cells by LC–MS. The results of RT-PCR revealed that HS synthases, Ext1 and Ext2, were expressed in FBJ-S1 cells more than in FBJ-LL cells. Furthermore, siRNA against Ext1 suppressed the expression of HS and enhanced the motility of FBJ-S1 cells. In addition, the expression of heparanase (HPSE) was enhanced in Ext-1-knockdown FBJ-S1 cells, and responsible for the increase in cell motility caused by the down-regulation of Ext1 expression. Our data provide the first evidence that Ext1 regulates the expression of HPSE and also indicated that levels of Ext1 and HPSE influenced the motility of FBJ cells. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1475-8 Authors Yinan Wang, Department of Biosciences and Informatics, Keio University, Hiyoshi, Yokohama, 223-8522 Japan XiaoYan Yang, Laboratory of Tumor Biology and Glycobiology, Department of Life Sciences and Biopharmaceutics, Shenyang Pharmaceutical University, P.O. Box 29, 103 WenHua Road, Shenyang, 110016 Liaoning, People’s Republic of China Sadako Yamagata, Laboratory of Tumor Biology and Glycobiology, Department of Life Sciences and Biopharmaceutics, Shenyang Pharmaceutical University, P.O. Box 29, 103 WenHua Road, Shenyang, 110016 Liaoning, People’s Republic of China Tatsuya Yamagata, Laboratory of Tumor Biology and Glycobiology, Department of Life Sciences and Biopharmaceutics, Shenyang Pharmaceutical University, P.O. Box 29, 103 WenHua Road, Shenyang, 110016 Liaoning, People’s Republic of China Toshinori Sato, Department of Biosciences and Informatics, Keio University, Hiyoshi, Yokohama, 223-8522 Japan Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    This study explored the role of pro- and anti-inflammatory cytokines in dimethyl benz(a)anthracene (DMBA)-induced lung cancer and its subsequent correction with a COX-2 inhibitory NSAID, etoricoxib. A single dose of DMBA (20 mg/kg body weight) in 0.9 % NaCl administered intratracheally was used to induce tumors in the rat lungs in 20 weeks. The study of pro-inflammatory cytokines like IL-1β, TNF-α, and IFN-γ revealed their upregulation by DMBA administration and restoration of their levels toward normal by the treatment with etoricoxib, while the anti-inflammatory cytokine IL-2 was found to be down-regulated with carcinogen administration and corrected with etoricoxib treatment. Apoptosis was studied by mitochondrial Bcl-2/Bax ratio and staining with fluorescent dyes acridine orange/ethidium bromide. The results showed a decreased apoptotic level with DMBA which was corrected with etoricoxib. Also, mitochondrial membrane potential was studied using JC-1 and rhodamine-123, which are membrane permeant fluorescent dyes, and generate information about cells at lower and higher mitochondrial membrane potential (∆Ψ M ). The results showed the presence of maximum number of cells with higher ∆Ψ M in the DMBA group and their number was considerably lowered in the other three groups. Content Type Journal Article Pages 1-12 DOI 10.1007/s11010-012-1451-3 Authors Neeti Nadda, Department of Biophysics, Panjab University, Chandigarh, 160014 India Shruti Setia, Department of Biophysics, Panjab University, Chandigarh, 160014 India Vivek Vaish, Department of Biophysics, Panjab University, Chandigarh, 160014 India Sankar Nath Sanyal, Department of Biophysics, Panjab University, Chandigarh, 160014 India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The metal-insulator transition for the square, simple cubic, and body centered cubic lattices has been studied within the Hubbard model at half-filling taking into account nearest- and next-nearest-neighbor electron hopping. Both staggered antiferromagnetic and incommensurate magnetic states (spin-spiral wave) have been considered. The inclusion of the latter states for the three-dimensional lattices does not change the general pattern of the metal-insulator transition, but opens the fundamentally new possibility of the metal-insulator transition of the first order between the magnetically ordered states for the square lattice. Content Type Journal Article Category Condensed Matter Pages 171-175 DOI 10.1134/S002136401215012X Authors M. A. Timirgazin, Physical-Technical Institute, Ural Branch, Russian Academy of Sciences, Izhevsk, 426000 Russia A. K. Arzhnikov, Physical-Technical Institute, Ural Branch, Russian Academy of Sciences, Izhevsk, 426000 Russia V. Yu. Irkhin, Physical-Technical Institute, Ural Branch, Russian Academy of Sciences, Izhevsk, 426000 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 3

Beschreibung:    Precision measurements of the real and imaginary parts of the microwave surface impedance Z ac ( T ) = R ac ( T ) + iX ac ( T ) of the conducting ac layers of the k -(BEDT-TTF) 2 Cu[N(CN) 2 ]Br crystals in the temperature interval of 0.5 〈 T 〈 100 K have demonstrated a series of features: (i) the temperature course of the field penetration depth is close to linear Δλ ac ( T )∞Δ X ac ( T ) in the superconducting state at T T c ∼ 11.5 K; (ii) the curves R ac ( T ) = X ac ( T ) coincide at T c 〈 T 〈 40 K; (iii) the X ac ( T ) value at T 〉 40 K increases in comparison with R ac ( T ); (iv) the dependence R ac ( T ) at T 〉 40 K is nonmonotonic in thin crystals. These features of the impedance Z ac ( T ) with increasing T are interpreted in terms of (i) the d -type symmetry of the superconducting order parameter, (ii) normal skin effect, (iii) manifestations of the antiferromagnetic fluctuations, and (iv) the size effect. The electrodynamic parameters of k -(BEDT-TTF) 2 Cu[N(CN) 2 ]Br have been determined. Content Type Journal Article Category Condensed Matter Pages 184-187 DOI 10.1134/S0021364012150088 Authors N. V. Perunov, Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia A. F. Shevchun, Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia N. D. Kushch, Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia M. R. Trunin, Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, 142432 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 3

Beschreibung:    The experimental conditions that facilitate the excitation of parametric decay instabilities upon the electron cyclotron resonance heating of a plasma at the second harmonic extraordinary wave in tokamaks and stellarators and, as a result, make anomalous absorption of microwave power possible have been analyzed. It has been shown that, in the case of a nonmonotonic radial profile of the plasma density, when the beam of electron cyclotron waves passes near the equatorial plane of a toroidal device, the parametric excitation of electron Bernstein waves, as well as the generation of ion Bernstein waves propagating from the parametric decay region to the nearest ion cyclotron harmonic, where they efficiently interact with ions, is possible. The proposed theoretical model can explain the anomalous generation of accelerated ions observed upon electron cyclotron heating in small and moderate toroidal facilities. Content Type Journal Article Category Plasma, Hydro- and Gas Dynamics Pages 164-170 DOI 10.1134/S002136401215009X Authors A. Yu. Popov, Ioffe Physical Technical Institute, Russian Academy of Sciences, Politekhnicheskaya ul. 26, St. Petersburg, 194021 Russia E. Z. Gusakov, Ioffe Physical Technical Institute, Russian Academy of Sciences, Politekhnicheskaya ul. 26, St. Petersburg, 194021 Russia A. N. Saveliev, Ioffe Physical Technical Institute, Russian Academy of Sciences, Politekhnicheskaya ul. 26, St. Petersburg, 194021 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 3

Beschreibung:    The connection of short-term neutron bursts near sea level with the electric and geomagnetic atmospheric fields during thunderstorms in 2009–2011 has been experimentally studied. The data from the cosmic-ray spectrograph named after Kuzmin, an electrostatic fluxmeter, and a three-component fluxgate magnetometer in Yakutsk have been analyzed. It has been shown that short-term (no longer than 4 min) neutron bursts are due to negative lightning discharges. The bursts are detected at the ground level 1–3 km below thunderstorm clouds. In this case, the neutron flux is about 4 × 10 −3 cm −2 s −1 . The minimum energy of the neutrons that are efficiently detected by the monitor is about 10 MeV. It has been found that short-term neutron bursts are detected when the electric field strength reaches a threshold value of −16 kV/m. Content Type Journal Article Category Miscellaneous Pages 188-191 DOI 10.1134/S0021364012150106 Authors S. A. Starodubtsev, Shafer Institute of Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677980 Russia V. I. Kozlov, Shafer Institute of Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677980 Russia A. A. Toropov, Shafer Institute of Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677980 Russia V. A. Mullayarov, Shafer Institute of Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677980 Russia V. G. Grigor’ev, Shafer Institute of Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677980 Russia A. V. Moiseev, Shafer Institute of Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677980 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 3

Beschreibung:    We have derived the so-called gap equation, which determines the upper critical magnetic field, perpendicular to conducting chains of a quasi-one-dimensional superconductor. By analyzing this equation at low temperatures, we have found that the calculated angular dependence of the upper critical magnetic field is qualitatively different than that in the so-called effective mass model. In particular, our theory predicts a non-analytical angular dependence of the upper critical magnetic field, H c 2 (0) − H c 2 (α) ∼ α 3/2 , when magnetic field is close to some special crystallographic axis and makes an angle α with it. We discuss possible experiments on the superconductor (DMET) 2 I 3 to discover this non-analytical dependence. Content Type Journal Article Category Condensed Matter Pages 176-180 DOI 10.1134/S0021364012150052 Authors A. G. Lebed, Department of Physics, University of Arizona, AZ 85721, Tucson, USA O. Sepper, Department of Physics, University of Arizona, AZ 85721, Tucson, USA Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 96 Journal Issue Volume 96, Number 3

Beschreibung:    With the increase of environment temperature, more and more attentions are payed to the effects of heat stress. Cells under heat shock either are adapted to the condition or are damaged and dead. In this paper, we found that heat shock induced endoplasmic reticulum (ER) stress. ATF4, PERK, and IRE1α were induced by heat shock of 45 °C in the transcriptional level. Under the stress of 45 °C, PERK was phosphorylated and XBP1s was detected. The result indicated that heat shock could induce the ER stress. We found that heat shock of 45 °C induced the dysregulation of HSP70 and DNA-PKcs, and downregulated the expression of PARP1 and XRCC1. Further results showed that after the knockdown of ATF4 or IRE1α, the expression of DNA-PKcs and XRCC1 were increased. It was indicated that ATF4 and IRE1α could inhibit the expression of DNA-PKcs and XRCC1 under the heat stress. Our results suggested that heat shock could activate ER stress. IRE1α and ATF4, as the important ER stress molecules, could inhibit the expression of DNA repair proteins DNA-PKcs, XRCC1, and HSP70 under heat shock. Downregulation of DNA repair proteins could aggravate the cell damage that may cause cell apoptosis. This may explain that heat shock could increase the lethality of chemotherapeutic drugs on tumor cells. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1439-z Authors Huifang Zhu, Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, 400016 China Feng-Jin Guo, Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, 400016 China Wenjun Zhao, Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, 400016 China Jinghua Zhou, Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, 400016 China Yanna Liu, Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, 400016 China Fangzhou Song, Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, 400016 China Yingxiong Wang, Laboratory of Reproductive Biology, Chongqing Medical University, Chongqing, 400016 China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Placental growth factor (PlGF) inhibition produced promising results in reducing tumor burden in a diethylnitrosamine (DEN)-induced mouse model for hepatocellular carcinoma (HCC). The aim of this study was to non-invasively assess the improved histology by performing a serum glycomic analysis. To elucidate the molecular mechanism underlying the observed glycomic effects, we investigated the transcription and expression of E26 transformation-specific sequence 1 (Ets-1), a transcription factor essential for the glycomic and angiogenic changes in malignant transformation, including its different phosphorylated forms that result from activation of the MAP kinase and a Ca 2+ -dependent pathway. In addition, three Ets-1-dependent glycosyltransferase genes, Mgat4a , Mgat4b , and Mgat5 , were also evaluated. HCC was induced in mice by weekly injections with DEN for 16, 20, 25, and 30 w. In the treatment study, mice were injected with DEN for 25 w and subsequently treated with PlGF antibodies (5D11D4) for 5 w. Finally, PlGF−/− mice were injected with DEN for 20, 25, and 30 w. Serum N -glycans were analyzed with DNA sequencer-assisted fluorophore-assisted capillary electrophoresis and compared with histology. Maximum altered N -glycan phenotype was reached after 20 w of DEN-injections, i.e., when the first neoplastic lesions started to appear. 5D11D4-treatment improved the glycomic phenotype in that 7 of the 11 altered glycans tended to normalize. The PlGF−/− mice also showed a normalization trend, although not to the same extent of the treatment group. Number of Ets1 , Mgat4a , Mgat4b , and Mgat5 transcripts increased considerably in DEN-injected mice, however, a non-significant decrease was observed after 5D11D4-treatment. On the protein level, 5D11D4-treatment had a prominent effect on the MAP kinase pathway with a significant p38 activation, yet independent of Ets-1 function. Content Type Journal Article Pages 1-12 DOI 10.1007/s11010-012-1461-1 Authors Bram Blomme, Department of Hepatology and Gastroenterology, Ghent University Hospital, 9000 Ghent, Belgium Femke Heindryckx, Department of Hepatology and Gastroenterology, Ghent University Hospital, 9000 Ghent, Belgium Jean Marie Stassen, ThromboGenics NV, Leuven, Belgium Anja Geerts, Department of Hepatology and Gastroenterology, Ghent University Hospital, 9000 Ghent, Belgium Isabelle Colle, Department of Hepatology and Gastroenterology, Ghent University Hospital, 9000 Ghent, Belgium Hans Van Vlierberghe, Department of Hepatology and Gastroenterology, Ghent University Hospital, 9000 Ghent, Belgium Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    In the present study, the nephroprotective effect of gallic acid isolated from Peltiphyllum peltatum was examined in sodium fluoride (NaF) treated rats. Nephrotoxicity was induced by 1-week intoxication of NaF at 600 ppm through drinking water. The levels of thiobarbituric acid reactive substances, reduced glutathione as well as activities of superoxide dismutase and catalase in renal tissues homogenates were determined. The serum biochemical markers of renal injuries including creatinine, serum urea, blood urea nitrogen, uric acid levels as well as the levels of phosphate and calcium were also assessed. Intoxication with NaF caused a significant increase in the levels of thiobarbituric acid reactive substances (46 % versus to control) and reduced the glutathione concentration (47 %) and the activities of superoxide dismutase (46 %) and catalase (41 %) in renal tissues homogenates. NaF intoxication also induced significant alterations in the kidney biochemical markers increasing the levels of urea, uric acid, blood urea nitrogen, creatinine, and phosphate and decreasing the levels of calcium. Daily administration of gallic acid (20 mg/kg) for 1 week before NaF intoxication brought the antioxidant–oxidant balance similar to the NaF-untreated group. Silymarin, used a standard antioxidant agent, also showed a nephroprotective activity. We concluded that NaF caused nephrotoxicity and oxidative stress in renal tissues and daily administration of gallic acid for 1 week prior to intoxication inhibited toxicity and oxidative stress. Content Type Journal Article Pages 1-7 DOI 10.1007/s11010-012-1464-y Authors Seyed Mohammad Nabavi, Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Solomon Habtemariam, Pharmacognosy Research Laboratories, Medway School of Science, University of Greenwich, Central Avenue, Chatham-Maritime, Kent, ME4 4TB UK Seyed Fazel Nabavi, Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Antoni Sureda, Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Illes Balears, Spain Maria Daglia, Department of Drug Sciences, School of Pharmacy, University of Pavia, Pavia, Italy Akbar Hajizadeh Moghaddam, Department of Biology, University of Mazandaran, Babolsar, Iran Mohammad Ali Amani, Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Schwann cells are critically important in recovery from injuries to the peripheral nervous system, and their absence from the central nervous system (CNS) may be a critical limiting factor in the CNS regeneration capacity. Various types of stem cells have been investigated for their potential to be induced to develop a Schwann cell phenotype, with mesenchymal stem cells (MSCs) being the most promising among them. The methods for inducing MSCs differentiation into Schwann cell-like cells are presented in detail in this review. The evidence related to successful differentiation of MSCs to Schwann cell-like cells is particularly discussed herein, which includes the changes in morphology, phenotype, function, and proteome. The possible explanations for the differentiation of MSCs to Schwann cell-like cells are also presented. Finally, we suggest future research aims which will need to be fulfilled to elucidate the biology of Schwann cell differentiation and MSC transdifferentiation, to enable clinical application of therapeutic differentiated MSC transplantation into nerve injury sites. Content Type Journal Article Pages 1-9 DOI 10.1007/s11010-012-1351-6 Authors Yu Pan, Research Center of Medical Sciences, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 Dongchuan Road, Guangzhou, 510080 China Sa Cai, Department of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Integrins are transmembrane receptors that can specifically bind extracellular matrix (ECM) proteins. Assembly of the ECM protein fibronectin into fibrils has been shown to be a cell-mediated process that requires integrins. Like fibronectin, fibrillin 1 is an ECM glycoprotein that can assemble into fibrils, but the role of integrins in fibril formation is not understood. To investigate the role of integrins in fibrillin 1 ECM deposition, cells that normally produce and assemble fibrillin 1 fibers in vitro were stably transfected with plasmid constructs encoding short interfering RNAs that target specific integrin subunits. Cells that were deficient in α2- and β3-integrin subunits produced and deposited fibronectin normally, but cells that were deficient for α5 and αV were unable to elaborate a fibronectin matrix, although they continued to produce and secrete the protein. Surprisingly, the cells that were unable to elaborate a fibronectin matrix also lost fibrillin 1 gene expression. Content Type Journal Article Pages 1-12 DOI 10.1007/s11010-012-1383-y Authors Rajeev K. Boregowda, Division of Musculoskeletal Sciences, Department of Orthopaedics, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA Brooke M. Krovic, Division of Musculoskeletal Sciences, Department of Orthopaedics, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA Timothy M. Ritty, Division of Musculoskeletal Sciences, Department of Orthopaedics, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    In our tunneling investigation using Andreev superconductor-normal metal-superconductor contacts on LiFeAs single crystals we observed two reproducible independent subharmonic gap structures at dynamic conductance characteristics. From these results, we can derive the energy of the large superconducting gap Δ L = (2.5–3.4) meV and the small gap Δ S = (0.9–1) meV at T = 4.2 K for the T C local ≈ (10.5–14) K (the contact area critical temperature which deviation causes the variation of Δ L ). The BCS-ratio is found to be 2Δ L / k B T C = 4.6–5.6, whereas 2Δ S / k B T C ≪ 3.52 results from induced superconductivity in the bands with the small gap. Content Type Journal Article Category Condensed Matter Pages 537-543 DOI 10.1134/S0021364012100086 Authors S. A. Kuzmichev, Moscow State University, Moscow, 119991 Russia T. E. Shanygina, Moscow State University, Moscow, 119991 Russia I. V. Morozov, Moscow State University, Moscow, 119991 Russia A. I. Boltalin, Moscow State University, Moscow, 119991 Russia M. V. Roslova, Moscow State University, Moscow, 119991 Russia S. Wurmehl, IFW-Dresden, Institute for Solid State Research, D-01171 Dresden, Germany B. Büchner, IFW-Dresden, Institute for Solid State Research, D-01171 Dresden, Germany Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 95 Journal Issue Volume 95, Number 10

Beschreibung:    Although the lipid mediator sphingosine 1-phosphate (S1P) has been identified to induce cell growth arrest of human keratinocytes, the sphingolipid effectively protects these epidermal cells from apoptosis. The molecular mechanism of the anti-apoptotic action induced by S1P is less characterized. Apart from S1P, endogenously produced nitric oxide (NO • ) has been recognized as a potent modulator of apoptosis in keratinocytes. Therefore, it was of great interest to elucidate whether S1P protects human keratinocytes via a NO • -dependent signalling pathway. Indeed, S1P induced an activation of endothelial nitric oxide synthase (eNOS) in human keratinocytes leading to an enhanced formation of NO • . Most interestingly, the cell protective effect of S1P was almost completely abolished in the presence of the eNOS inhibitor L-NAME as well as in eNOS-deficient keratinocytes indicating that the sphingolipid metabolite S1P protects human keratinocytes from apoptosis via eNOS activation and subsequent production of protective amounts of NO • . It is well established that most of the known actions of S1P are mediated by a family of five specific G protein-coupled receptors. Therefore, the involvement of S1P-receptor subtypes in S1P-mediated eNOS activation has been examined. Indeed, this study clearly shows that the S1P 3 is the exclusive receptor subtype in human keratinocytes which mediates eNOS activation and NO • formation in response to S1P. In congruence, when the S1P 3 receptor subtype is abrogated, S1P almost completely lost its ability to protect human keratinocytes from apoptosis. Content Type Journal Article Pages 1-12 DOI 10.1007/s11010-012-1433-5 Authors Elisabeth I. Schmitz, Institute of Nutritional Science, Toxicology, Faculty of Mathematics and Natural Science, University of Potsdam, Arthur-Scheunert-Allee, 114-116, 14558 Nuthetal, Germany Henrik Potteck, Institute of Pharmacy, Pharmacology and Toxicology, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195 Berlin, Germany Melanie Schüppel, Institute of Pharmacy, Pharmacology and Toxicology, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195 Berlin, Germany Marianti Manggau, Department of Pharmacology, Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar, 90245 Indonesia Elly Wahydin, Department of Pharmacology, Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar, 90245 Indonesia Burkhard Kleuser, Institute of Nutritional Science, Toxicology, Faculty of Mathematics and Natural Science, University of Potsdam, Arthur-Scheunert-Allee, 114-116, 14558 Nuthetal, Germany Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The present study was conducted to investigate if anti-inflammatory drug aspirin could alter the cytotoxic action of cisplatin on tumor cells. Using a transplantable T cell lymphoma in a murine model, we demonstrate that exposure to aspirin exerts a priming action on tumor cells, rendering them susceptible to induction of cell death by cisplatin with consequences on retardation of tumor progression. The priming action of aspirin on tumor cells was found to be dependent on an altered constitution of tumor microenvironment with respect to decline of acidosis and modulation in the expression of cell cycle and survival regulatory molecules like cyclin B1, cyclin D, bcl-2, bcl-xL, p53, and cytokines: IL-4, IL-10, IFN- γ & VEGF. The study also discusses possible mechanisms underlying augmentary action of aspirin on cisplatin-mediated tumor cells killing. This is the first report showing that pre-exposure of tumor cells to aspirin lowers the concentration of cisplatin to exert its cytotoxic action. The finding of this study will help in designing novel antitumor protocols with reduced dose of cisplatin. Content Type Journal Article Pages 1-12 DOI 10.1007/s11010-012-1421-9 Authors Anjani Kumar, School of Biotechnology, Banaras Hindu University, Varanasi, 221 005 Uttar Pradesh, India Sukh Mahendra Singh, School of Biotechnology, Banaras Hindu University, Varanasi, 221 005 Uttar Pradesh, India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Dendritic cells (DCs) are the most potent professional antigen-presenting cells and are involved in the initiation and progression of atherosclerosis. Recent data suggest that mature macrophages differentiate into dendritic-like cells when exposed to oxidized low-density lipoprotein (oxLDL). The purpose of the present study was to determine the effect of atorvastatin on the differentiation of macrophages to DCs and the molecular mechanisms of this transition. Mouse macrophage-like RAW264.7 cell was differentiated into a dendritic-like phenotype by incubation with oxLDL in the absence or presence of atorvastatin. The results showed that atorvastatin suppressed DC-like morphologic changes in vitro as assessed by decreased expression of DC maturation markers (CD83, CD11c, CD86, major histocompatibility complex class II, and CD1d). Atorvastatin also inhibited other oxLDL-induced functional changes including endocytic activity, ability to induce T cell proliferation, and cytokine secretion. Western blot analysis showed that oxLDL treatment of RAW264.7 cells induced phosphorylation of p38 mitogen-activated protein kinase (MAPK). However, blocking p38 MAPK with SB203580 significantly downregulated the expression of DC maturation markers, accompanied by decreased cytokine secretion. The findings of the present work demonstrate that that atorvastatin suppresses the oxLDL-induced DC-like differentiation of RAW264.7 cells by inactivating the p38 MAPK signaling pathway. Content Type Journal Article Pages 1-9 DOI 10.1007/s11010-012-1427-3 Authors Liu-hua Hu, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Tuo Zhang, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Qin Shao, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Dan-dan Li, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Shu-xuan Jin, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Peng Nie, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Jing Yi, Department of Cell Biology, Key Laboratory of the Education Ministry for Cell Differentiation and Apoptosis, Institutes of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 China Ben He, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Ling-hong Shen, Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127 China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. Here, we tried to elucidate the possible neuroprotective effect of Salvigenin, a natural polyphenolic compound, on oxidative stress-induced apoptosis and autophagy in human neuroblastoma SH-SY5Y cells. We measured cell viability by MTT test and found that 25 μM is the best protective concentration of Salvigenin. GSH and SOD assays suggested that Salvigenin activates antioxidant factors. At the same time, measurement of ER stress-associated proteins including calpain and caspase-12 showed the ability of Salvigenin to decrease ER stress. We found that Salvigenin could decrease the apoptotic factors. Salvigenin inhibited H 2 O 2 -induced caspase-3 which is a hallmark of apoptosis in addition to reducing Bax\Bcl-2 ratio by 1.45 fold. Additionally, Salvigenin increased the levels of autophagic factors. Our results showed an increase in LC3-II/LC3-I ratio, Atg7, and Atg12 in the presence of 25 μM of Salvigenin by about 1.28, 1.25, and 1.54 folds, respectively, compared to H 2 O 2 -treated cells. So it seems that H 2 O 2 cytotoxicity mainly results from apoptosis. Besides, Salvigenin helps cells to survive by inhibiting apoptosis and enhancing autophagy that opens a new horizon for the future experiments. Content Type Journal Article Pages 1-14 DOI 10.1007/s11010-012-1416-6 Authors Ghazaleh Rafatian, Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Fariba Khodagholi, Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mahdi Moridi Farimani, Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran Shahnaz Babaei Abraki, Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mossa Gardaneh, Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Apigenin is a naturally occurring plant flavone with strong anti-oxidant and anti-inflammatory activity. While the anticancer properties of Apigenin have been extensively studied, little is known about its effects on endothelial dysfunction. We investigated the effects of Apigenin in EAhy926 endothelial cells exposed to TNFα by evaluating the expression of eNOS and MMP-9, two key molecules in endothelial dysfunction. MMP-9 activity was measured by gel zymography. Western blot analysis was performed to analyze eNOS expression and signal transduction. Treatment with Apigenin (50 μM) counteracted the TNFα-induced expression of eNOS and MMP-9 and the TNFα- triggered activation of Akt, p38MAPK and JNK signalling suggesting that multiple signalling pathways are involved in mediating the protective effects of Apigenin on endothelial function. To better understand the molecular mechanisms underlying the protective effects of Apigenin, we used a pharmacological approach with specific inhibitors. The use of an Akt inhibitor mimicked the inhibitory effects of Apigenin on eNOS and MMP-9 expression, suggesting that eNOS and MMP-9 induction by TNFα depends on Akt activation. The TNFα-induced expression of MMP-9 was also affected by the JNK inhibitor SP600125. No effect on eNOS and MMP-9 expression was observed in the presence of the p38MAPK inhibitor SB203580 or the ERK 1/2 inhibitor PD98059. Pretreatment with ‘classic’ (ERα and ERβ) or ‘non classic’ (GPR30) oestrogen receptor (ER) inhibitors (ICI182,780 and PTX, respectively) counteracted the ability of Apigenin to decrease the TNFα-triggered activation of the Akt pathway. Consistently, the use of both ER inhibitors reversed the inhibitory effects of Apigenin on the TNFα-induced expression of eNOS and, to a lesser extent, MMP-9. We can conclude that Apigenin exerts its inhibitory effect on the TNFα-induced expression of eNOS and MMP-9 through the Akt signalling inhibition generated by ER activation. Oestrogen signalling has been implicated in protection from cardiovascular disease. Therefore, having regard to its ability to bind to ERs, Apigenin may be considered an oestrogen-like molecule to potentially be used against the onset and progression of vascular diseases associated with endothelial dysfunction. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1429-1 Authors Daniela Palmieri, Vascular and Endovascular Unit, Laboratory of Clinical and Experimental Vascular Biology, DISC, University Hospital IRCCS San Martino, Largo Rosanna Benzi 8, 16132 Genoa, Italy Patrizia Perego, Department of Chemical and Process Engineering “G.B. Bonino”, University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy Domenico Palombo, Vascular and Endovascular Unit, Laboratory of Clinical and Experimental Vascular Biology, DISC, University Hospital IRCCS San Martino, Largo Rosanna Benzi 8, 16132 Genoa, Italy Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Human atrial and ventricular myocardium has distinct structure and physiology. MicroRNAs (miRNAs) are the central players in the regulation of gene expression, participating in many physiological processes. A comprehensive knowledge of miRNA expression in the human heart is essential for the understanding of myocardial function. The aim of this study was to compare the miRNA signature in human right atrial and ventricular myocardium. Agilent human miRNA arrays were used to indicate the miRNA expression signatures of the right atrial ( n  = 8) and ventricular ( n  = 9) myocardium of healthy individuals. Quantitative reverse transcription-polymerase chain reactions (qRT-PCRs) were used to validate the array results. DIANA-mirPath was used to incorporate the miRNAs into pathways. MiRNA arrays showed that 169 miRNAs were expressed at different levels in human right atrial and ventricular myocardium. The unsupervised hierarchical clustering analysis based on the 169 dysregulated miRNAs showed that miRNA expression categorized two well-defined clusters that corresponded to human right atrial and ventricular myocardium. The qRT-PCR results correlated well with the microarray data. Bioinformatic analysis indicated the potential miRNA targets and molecular pathways. This study indicates that distinct miRNA expression signatures in human right atrial and ventricular myocardium. The findings provide a novel understanding of the molecular differences between human atrial and ventricular myocardium and may establish a framework for an anatomically detailed evaluation of cardiac function regulation. Content Type Journal Article Pages 1-7 DOI 10.1007/s11010-012-1417-5 Authors Yangyang Zhang, Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 People’s Republic of China Xiaowei Wang, Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 People’s Republic of China Xiaohan Xu, Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 People’s Republic of China Jun Wang, Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 People’s Republic of China Xiang Liu, Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 People’s Republic of China Yijiang Chen, Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The lactate dehydrogenase (LDH) has been studied widely because it exists in various isozymic forms. The association of A and B subunits of LDH can generate five tetrameric isozymes, but the finding of the sixth isozyme in mature human testis and sperm indicated the presence of an additional subunit of LDH, designated as LDH-X (also termed LDH-C4 due to tetrameric nature of C-subunit). LDH-C4 isozyme is an iso-, allo-, and auto-antigen present in mammalian sperm cells. The synthesis of LDH-C4 in the testis takes place during sexual maturation, and it is the predominant fraction in mature spermatozoa. Though, originally considered to be testis specific, LDH-C or Ldh3 in mice was later detected in the murine oocyte and early embryo. Ldh3 in mouse supports its role in energy production in spermatids that favor lactate as substrate and in spermatozoa with a characteristic aerobic glycolytic path to yield ATP. During last two decades, cancer/testis-associated genes (CTAs) which are expressed only in the germinal epithelium of the testis are also expressed in some cancer cells, but not in non-cancerous somatic tissues. The CTAs are considered promising candidates for diagnosis and immunotherapy of cancer. The sperm-specific Ldh - c gene has been shown to express in a broad spectrum of human tumors, with high frequency in lung cancer, melanoma, and breast cancer; the protein being expressed virtually in all tumor types tested. Accordingly, LDH-C4 is the unique target for contraception in both males and females and offers potential future for immunotherapy of different types of cancers. As LDH-C has a preference for lactate as a substrate, LDH-C activation in cancer may depend on lactate for ATP production. The major aim of this article is to review the salient features of LDH-C subunit and the immune responses of LDH-C4 in homologous and heterologous species in relation to its role in acceptance or rejection of the allograft and its application in contraception and immunotherapy of cancer, directly or indirectly through the regulation of its substrate, the lactate. Content Type Journal Article Pages 1-13 DOI 10.1007/s11010-012-1428-2 Authors G. S. Gupta, Department of Biophysics, Panjab University, Chandigarh, 160014 India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Our aim was to examine correlations between polymorphisms in five antioxidant enzymes genes, activity of free-radical processes, and the risk of restenosis after coronary artery stenting with bare metal stents (BMS). A total of 101 male patients who underwent intracoronary stenting using BMS and coronary angiography follow-up of 6 months were enrolled in: group with in-stent restenosis ( n  = 44) and without restenosis ( n  = 57). The content of lipoperoxides and malondialdehyde (MDA) in Low-density lipoprotein (LDL), activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) in erythrocytes, and genotypes polymorphisms of the CAT gene (−262C/T), paraoxonase-1 ( PON - 1 ) gene (163T/A and 575A/G), endothelial nitric oxide synthase ( eNOS ) gene (298G/T (rs#1799983) and −786T/C), GPx - 1 gene (599C/T (rs#1050450)), and glutathione-S-transferase ( GSTP ) gene (313A/G) were determined. In carriers of the minor allele of 599C/T polymorphism of the GPx - 1 gene, activity of GPx in erythrocytes was lower by 17 % than in wild allele homozygotes, while the content of lipoperoxides in LDL was higher by 74 %. T-allele of 599C/T polymorphism of the GPx - 1 gene (OR = 2.9; 95 % CI: 1.23–6.82) and T-allele of 298G/T polymorphism of the eNOS gene (OR = 2.79; 95 % CI: 1.17–6.66) were associated with the risk of in-stent restenosis. Minor alleles of polymorphisms 298G/T of the eNOS gene and 599C/T of the GPx - 1 gene are associated with an increased risk of in-stent restenosis. Minor allele of the GPx - 1 gene 599C/T polymorphism leads to a decrease of the GPx activity and increase of the activity of free-radical processes. Content Type Journal Article Pages 1-9 DOI 10.1007/s11010-012-1419-3 Authors Yu. A. Shuvalova, Department of Atherosclerosis Problems, Russian Cardiology Research and Production Complex of the Russian Federation Ministry of Health and Social Development, 3rd Cherepkovskaya Street, 15a, Moscow, 121552 Russia A. I. Kaminnyi, Department of Atherosclerosis Problems, Russian Cardiology Research and Production Complex of the Russian Federation Ministry of Health and Social Development, 3rd Cherepkovskaya Street, 15a, Moscow, 121552 Russia A. N. Meshkov, Department of Clinical Cardiology and Molecular Genetics, National Center of Preventive Medcine, Moscow, Russia R. O. Shirokov, Laboratory of Radio Vascular Therapy, Russian Cardiology Research and Production Complex of the Russian Federation Ministry of Health and Social Development, Moscow, Russia A. N. Samko, Laboratory of Radio Vascular Therapy, Russian Cardiology Research and Production Complex of the Russian Federation Ministry of Health and Social Development, Moscow, Russia Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    High-density lipoprotein (HDL) possesses protective properties in cardiovascular diseases. However, the effect of HDL on the mesenchymal stem cells (MSCs), which could be mobilized to the damaged myocardial tissue, has not been well elucidated yet. In the current study, we investigated the effect of HDL on the proliferation of MSCs so as to reveal its molecular mechanisms. MSCs derived from rats were treated with HDL in different concentrations and for different periods. The proliferation of MSCs was measured with MTT and BrdU cell proliferation assay. The phosphorylation of Akt, ERK1/2 and the expression of p21 were evaluated by Western blotting. After the activity of respective pathways was down-regulated by the specific inhibitor and the gene of scavenger receptor-B type I (SR-BI) was knocked down by RNA interference, BrdU assay was performed to examine this effect of HDL on MSCs. We found that the proliferation of MSCs induced by HDL, in a time- and concentration-dependent manner, was the phosphorylation of Akt- and ERK1/2-dependent, which was significantly attenuated by the specific inhibitor to respective pathways. Moreover, MAPK/ERK1/2 pathway exerted a more dominating effect on this process. SR-BI contributed to HDL-induced proliferation of MSCs, which was effectively abolished by the silencing of SR-BI. The results suggested that HDL was capable of improving MSCs proliferation, in which MAPK/ERK1/2 and PI3K/Akt pathways involved and SR-BI played a critical role as well. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1422-8 Authors Jianfeng Xu, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Juying Qian, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Xinxing Xie, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Li Lin, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Jianying Ma, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Zheyong Huang, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Mingqiang Fu, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Yunzeng Zou, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Junbo Ge, Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032 China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    High levels of homocysteine (Hcy), known as hyperhomocysteinemia (HHcy), are associated with cerebrovascular diseases, such as vascular dementia, stroke, and Alzheimer’s disease. The γ-amino butyric acid (GABA) is an inhibitory neurotransmitter and a ligand of GABA-A receptor. By inhibiting excitatory response, it may decrease complications associated with vascular dementia and stroke. Hcy specifically competes with the GABA-A receptors and acts as an excitotoxic neurotransmitter. Previously, we have shown that Hcy increases levels of NADPH oxidase and reactive oxygen species (ROS), and decreases levels of thioredoxin and peroxiredoxin by antagonizing the GABA-A receptor. Hcy treatment leads to activation of matrix metalloproteinases (MMPs) in cerebral circulation by inducing redox stress and ROS. The hypothesis is that Hcy induces MMPs and suppresses tissue inhibitors of metalloproteinase (TIMPs), in part, by inhibiting the GABA-A receptor. This leads to degradation of the matrix and disruption of the blood brain barrier. The brain cortex of transgenic mouse model of HHcy (cystathionine β-synthase, CBS−/+) and GABA-A receptor null mice treated with and without muscimol (GABA-A receptor agonist) was analysed. The mRNA levels were measured by Q-RT-PCR. Levels of MMP-2, -9, -13, and TIMP-1, -2, -3, and -4 were evaluated by in situ labeling and PCR-gene arrays. Pial venular permeability to fluorescence-labeled albumin was assessed with intravital fluorescence microscopy. We found that Hcy increases metalloproteinase activity and decreases TIMP-4 by antagonizing the GABA-A receptor. The results demonstrate a novel mechanism in which brain microvascular permeability changes during HHcy and vascular dementias, and have therapeutic ramifications for microvascular disease in Alzheimer’s patients. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1425-5 Authors David Lominadze, Department of Physiology and Biophysics, University of Louisville School of Medicine, Bldg. A, Room 1115, 500 South Preston Street, Louisville, KY, 40202, USA Neetu Tyagi, Department of Physiology and Biophysics, University of Louisville School of Medicine, Bldg. A, Room 1115, 500 South Preston Street, Louisville, KY, 40202, USA Utpal Sen, Department of Physiology and Biophysics, University of Louisville School of Medicine, Bldg. A, Room 1115, 500 South Preston Street, Louisville, KY, 40202, USA Alexander Ovechkin, Department of Physiology and Biophysics, University of Louisville School of Medicine, Bldg. A, Room 1115, 500 South Preston Street, Louisville, KY, 40202, USA Suresh C. Tyagi, Department of Physiology and Biophysics, University of Louisville School of Medicine, Bldg. A, Room 1115, 500 South Preston Street, Louisville, KY, 40202, USA Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    It has been shown that counterpropagating electromagnetic waves with different frequencies generate two lattices in a cubically nonlinear medium, one of which moves at a superluminal velocity. When weak radiation reflects from the superluminal lattice, the wavefront is quasi-conjugated with distortions owing to the Doppler frequency shift. These effects occur both in insulators with fast nonlinearity and in an electron-positron vacuum. Content Type Journal Article Category Optics and Laser Physics Pages 609-612 DOI 10.1134/S0021364012120132 Authors N. N. Rosanov, Vavilov State Optical Institute, Birzhevaya liniya 12, St. Petersburg, 199034 Russia Journal JETP Letters Online ISSN 1090-6487 Print ISSN 0021-3640 Journal Volume Volume 95 Journal Issue Volume 95, Number 12

Beschreibung:    The human papilloma virus E4 protein is highly expressed in late times of infection. Evidence to date suggests that E4 is essential for amplification of the viral genome and that it can influence cell cycle. Examination of the sequences encoding the E4 proteins from several genotypes of human papillomavirus revealed the presence of RXL-containing motifs reminiscent of the cyclin-binding motifs that have been identified in several cyclin-binding proteins. When baculovirus-produced human cyclin E and cyclin A with cdk2 were incubated in vitro with a GST-E4 fusion protein, both cyclin E and A stably interacted with the GST-E4 protein containing the full E4 sequence from HPV18. The interaction was not dependent on the presence of the kinase subunit but was dependent on the integrity of the RXL motif in E4. When incubated with cell extracts from the C33A human cervical carcinoma cell line or when expressed in C33A cells, the GST-E4 protein formed interactions with cyclin A and cdk2 and kinase activity could be demonstrated in the GST-E4 complex. In contrast to the baculovirus-produced cyclin E, cellular cyclin E failed to detectably interact with GST-E4 suggesting that the HPV18 E4 sequences are capable of interacting only with cyclin A in mammalian cells. These observations suggest that human papillomavirus E4 proteins can interact with cyclin A/cdk2, which may contribute to viral manipulation of the host cell cycle. Content Type Journal Article Pages 1-12 DOI 10.1007/s11010-012-1472-y Authors Qingming Ding, Department of Pathology and Molecular Medicine, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada Lili Li, Department of Pathology and Molecular Medicine, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada Peter Whyte, Department of Pathology and Molecular Medicine, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Atherosclerosis is one of the major complications of diabetes and involves endothelial dysfunction, matrix alteration, and most importantly migration and proliferation of vascular smooth muscle cells (VSMCs). Although hyperglycemia and hyperinsulinemia are known to contribute to atherosclerosis, little is known about the specific cellular signaling pathways that mediate the detrimental hyperinsulinemic effects in VSMCs. Therefore, we investigated the cellular mechanisms of hyperinsulinemia-induced migration and proliferation of VSMCs. VSMCs were treated with insulin (100 nM) for 6 days and subjected to various physiological and molecular investigations. VSMCs subjected to hyperinsulinemia exhibited increased migration and proliferation, and this is paralleled by oxidative stress [increased NADPH oxidase activity, NADPH oxidase 1 mRNA expression, and reactive oxygen species (ROS) generation], alterations in mitochondrial physiology (membrane depolarization, decreased mitochondrial mass, and increased mitochondrial ROS), changes in mitochondrial biogenesis-related genes (mitofusin 1, mitofusin 2, dynamin-related protein 1, peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma coactivator 1-beta, nuclear respiratory factor 1, and uncoupling protein 2), and increased Akt phosphorylation. Diphenyleneiodonium, a known NADPH oxidase inhibitor significantly inhibited migration and proliferation of VSMCs and normalized all the above physiological and molecular perturbations. This study suggests a plausible crosstalk between mitochondrial dysfunction and oxidative stress under hyperinsulinemia and emphasizes counteracting mitochondrial dysfunction and oxidative stress as a novel therapeutic strategy for atherosclerosis. Content Type Journal Article Pages 1-11 DOI 10.1007/s11010-012-1478-5 Authors Shiny Abhijit, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, Gopalapuram, Chennai, 600 086 India Regin Bhaskaran, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, Gopalapuram, Chennai, 600 086 India Abirami Narayanasamy, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, Gopalapuram, Chennai, 600 086 India Anand Chakroborty, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, Gopalapuram, Chennai, 600 086 India Nagaraj Manickam, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, Gopalapuram, Chennai, 600 086 India Madhulika Dixit, Department of Biotechnology, Indian Institute of Technology, Chennai, India Viswanathan Mohan, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, Gopalapuram, Chennai, 600 086 India Muthuswamy Balasubramanyam, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, Gopalapuram, Chennai, 600 086 India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Quercetin has been reported to protect testicular cells from oxidative damage induced by environmental chemicals. In this study, we isolated interstitial Leydig cells (ILCs) from immature rats, set-up ILCs culture, co-treated cells with atrazine (ATZ) and quercetin (QT), evaluated toxicity, and measured the expression levels of antioxidant enzymes and nuclear factor-kappaB (NF-κB) and levels of steroidogenic enzymes. ATZ decreased ILCs viability at concentrations higher than 10 μg/mL and increased reactive oxygen species, malondialdehyde (MDA), and glutathione levels. ATZ also increased glutathione peroxidase, glutathione reductase, and glutathione-S-transferase and decreased superoxide dismutase-1 (s od1 ) and superoxide dismutase-2 (s od2 ) messenger RNA (mRNA) levels which were prevented by QT. The changes in the MDA levels and lactate dehydrogenase leakage induced by ATZ (50 μg/mL) were also prevented on co-treatment with QT (50 μM). Furthermore, ATZ-induced 3β- and 17β-hydroxysteroid dehydrogenase activities and NF-κB-expressions at the mRNA and protein levels were also recovered to control value on co-treatment with QT. These data showed that QT protected against ATZ-induced ILCs toxicity by restoring the expression of NF-κB and steroidogenic activity and by preventing the oxidative stress. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1471-z Authors Sunny O. Abarikwu, Department of Chemical Sciences, College of Natural Sciences, Redeemer’s University, Redemption City, Ogun State, Nigeria Aditya B. Pant, Indian Institute of Toxicology Research (Formerly Industrial Toxicology Research Centre), Lucknow, India Ebenezer O. Farombi, Drug Metabolism and Toxicology Research laboratories, Department of Biochemistry, University of Ibadan, Ibadan, Oyo State, Nigeria Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Thrombin and platelet-derived growth factor-B chain (PDGF-B) are key factors in the stimulation of atherosclerosis. The effect of thrombin on PDGF-B production has been characterized. However, the underlying mechanism is still far clear. Here, we investigate the transcription factors and regulators that are involved in PDGF-B production caused by thrombin in endothelial cells (ECs). Levels of PDGF were analyzed by real-time RT-PCR and ELISA, while levels of early growth response-1 (Egr-1) were analyzed by real-time RT-PCR and western blot. To evaluate the function of CBP and Egr-1 involved in regulation of PDGF-B, small interfering RNA (siRNA) were used to down-regulate their expression in mRNA and protein level. Interaction of Egr-1 and CBP was measured with immunoprecipitation and western blot. Thrombin induced an early and transient up-regulation of transcription factor early Egr-1, which was followed by a delayed increase of PDGF-B. siRNA against Egr-1-inhibited thrombin-induced PDGF-B production. Furthermore, thrombin could enhance the interaction of Egr-1 with its co-activator CREB-binding protein (CBP). CBP knockdown attenuated this interaction, and led to a reduction of PDGF-B expression induced by thrombin. Our results suggest that CBP might be one of the main interaction targets for Egr-1, and the transient activation of Egr-1 and recruitment of CBP are required for thrombin-induced PDGF-B in ECs. Content Type Journal Article Pages 1-7 DOI 10.1007/s11010-012-1285-z Authors Jing Chen, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060 China Lin Xu, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060 China Sisi Chen, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060 China Jian Yang, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060 China Hong Jiang, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060 China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    We have previously reported Foxk1 as an important transcription factor in the myogenic progenitors. SWI-independent-3 (Sin3) has been identified as a Foxk1 binding candidate using a yeast two-hybrid screen. In the present study, we have identified the Foxk1 N-terminal (1–40) region as the Sin3 interacting domain (SID), and the PAH2 of Sin3 as the Foxk1 binding domain utilizing yeast two-hybrid and GST pull-down assays. Further studies revealed that knockdown of Sin3a or Sin3b results in cell cycle arrest and upregulation of cell cycle inhibitor genes. In summary, our present studies have shown that Foxk1 interacts with Sin3 through the SID and that Sin3 has an important role in the regulation of cell cycle kinetics of the MPC population. The results of these studies continue to define and assemble the networks that regulate the MPCs and muscle regeneration. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1302-2 Authors Xiaozhong Shi, Lillehei Heart Institute, University of Minnesota-Twin Cities, 4-108 NHH, 312 Church St SE, Minneapolis, MN 55455, USA Daniel J. Garry, Lillehei Heart Institute, University of Minnesota-Twin Cities, 4-108 NHH, 312 Church St SE, Minneapolis, MN 55455, USA Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Previous studies suggested that activated c-Src promote the tyrosine phosphorylation of NMDA receptor subunit NR2A, and thus aggravate the injury induced by transient cerebral ischemia/reperfusion (I/R) in rat hippocampus CA1 region. In this study, we examined the effect of nitric oxide (NO) on the activation of c-Src and the tyrosine phosphorylation of NMDA receptor NR2A subunit. The results show that S-nitrosylation and the phosphorylation of c-Src were induced after cerebral I/R in rats, and administration of nNOS inhibitor 7-NI, nNOS antisense oligonucleotides and exogenous NO donor sodium nitroprusside diminished the increased S-nitrosylation and phosphorylation of c-Src during cerebral I/R. The cysteine residues of c-Src modified by S-nitrosylation are Cys489, Cys498, and Cys500. On the other hand, NMDAR antagonist MK-801 could attenuate the S-nitrosylation and activation of c-Src. Taken together, the S-nitrosylation of c-Src is provoked by NO derived from endogenous nNOS, which is activated by Ca 2+ influx from NMDA receptors, and promotes the auto-phosphorylation at tyrosines and further phosphorylates NR2A. The molecular mechanism we outlined here is a novel postsynaptic NMDAR-nNOS/c-Src-mediated signaling amplification, the ‘NMDAR-nNOS → NO → SNO-c-Src → p-c-Src → NMDAR-nNOS’ cycle, which presents the possibility as a potential therapeutic target for stroke treatment. Content Type Journal Article Pages 1-15 DOI 10.1007/s11010-012-1280-4 Authors Li-Juan Tang, Research Center of Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002 Jiangsu, People’s Republic of China Chong Li, Research Center of Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002 Jiangsu, People’s Republic of China Shu-Qun Hu, Research Center of Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002 Jiangsu, People’s Republic of China Yong-Ping Wu, Jiangsu Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu, People’s Republic of China Yan-Yan Zong, Research Center of Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002 Jiangsu, People’s Republic of China Chang-Cheng Sun, Research Center of Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002 Jiangsu, People’s Republic of China Fa Zhang, Research Center of Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002 Jiangsu, People’s Republic of China Guang-Yi Zhang, Research Center of Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002 Jiangsu, People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Identifying low-abundance mutations is important for the therapy and diagnose of cancer. Since the potential for tumor heterogeneity, the efficient detection of cancer-relevant mutations largely depends on the sensitivity of the methods employed. To confirm whether the mutation detection platforms affect the perceived prevalence of the BRAF V600E and its correlation with clinicopathologic features in papillary thyroid carcinomas (PTC), we compared Sanger Sequencing (SS), Pyrosequencing (PS), and a newly built allele-specific real-time PCR (AS-qPCR) apparatus for the detection of BRAF V600E in a Chinese cohort of conventional variant PTC. Accurate plasmid standards were built to assess the limit of detection of the three platforms. In this research, AS-qPCR has been found both the most sensitive and reliable at detecting mutation. The mutations detected by AS-qPCR which were not detected by SS or PS due to low abundance were confirmed by mutation enrichment platform COLD-PCR followed by SS. When analyzed by AS-qPCR, BRAF V600E was associated with a more aggressive phenotype. Our results indicate that the reported prevalence of the BRAF V600E mutations in PTC has been underestimated and more sensitive methods such as AS-qPCR should be applied in clinical settings. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1282-2 Authors Weiqin Jiang, Cancer Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003 China Weibin Wang, Cancer Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003 China FangFang Fu, Institute of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003 China Xiaodong Teng, Department of Pathology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003 China Haohao Wang, Cancer Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003 China Haiyong Wang, Cancer Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003 China Lisong Teng, Cancer Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003 China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Although shortened telomeres were shown associated with several risk factors of diabetes, there is lack of data on their relationship with mitochondrial dysfunction. Therefore, we compared the relationship between telomere length and mitochondrial DNA (mtDNA) content in patients with type 2 diabetes mellitus (T2DM; n  = 145) and in subjects with normal glucose tolerance (NGT; n  = 145). Subjects were randomly recruited from the Chennai Urban Rural Epidemiology Study. mtDNA content and telomere length were assessed by Real-Time PCR. Malonodialdehyde, a marker of lipid peroxidation was measured by thiobarbituric acid reactive substances (TBARS) using fluorescence methodology. Adiponectin levels were measured by radioimmunoassay. Oxidative stress as determined by lipid peroxidation (TBARS) was significantly ( p  〈 0.001) higher in patients with T2DM compared to NGT subjects. In contrast, the mean telomere length, adiponectin and mtDNA content were significantly ( p  〈 0.001) lower in patients with T2DM compared to NGT subjects. Telomere length was positively correlated with adiponectin, HDL, mtDNA content and good glycemic/lipid control and negatively correlated with adiposity and insulin resistance. On regression analysis, shortened telomeres showed significant association with T2DM even after adjusting for waist circumference, insulin resistance, triglyceride, HDL, adiponectin, mtDNA & TBARS. mtDNA depletion showed significant association with T2DM after adjusting for waist circumference and adiponectin but lost its significance when further adjusted for telomere length, TBARS and insulin resistance. Our study emphasizes the clustering of accelerated aging features viz., shortened telomeres, decreased mtDNA content, hypoadiponectinemia, low HDL, and increased oxidative stress in Asian Indian type 2 diabetes patients. Content Type Journal Article Pages 1-8 DOI 10.1007/s11010-012-1276-0 Authors Finny Monickaraj, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Sankaramoorthy Aravind, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Kuppan Gokulakrishnan, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Chandrakumar Sathishkumar, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Paramasivam Prabu, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Durai Prabu, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Viswanathan Mohan, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Muthuswamy Balasubramanyam, Department of Cell and Molecular Biology, Madras Diabetes Research Foundation and Dr. Mohan’s Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai, 600 086 Tamilnadu, India Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Patients with type 2 diabetes (T2D) manifest significant abnormalities in lipoprotein structure and function. The deleterious impact of oxidative and glycoxidative modifications on HDL-mediated atheroprotective, antiinflammatory, and antioxidative phenomena has been well established. However, the biological effects of modified HDL on adrenal steroidogenesis—which could reveal a pathophysiological link to the overactivity of the renin–angiotensin–aldosterone system and its adverse cardiovascular consequences often observed in T2D—are not well delineated. We studied the role of modified HDL on aldosterone release from adrenocortical carcinoma cells (NCI-H295R). In vitro modifications of native HDL were performed in the presence of glucose for glycoxidized HDL (glycoxHDL) and sodium hypochlorite for oxidized HDL. Angiotensin II (AngII)-sensitized H295R cells were treated with lipoproteins for 24 h, and supernatant was used to measure aldosterone release. Both native and modified HDL augmented the steroid release from AngII-sensitized cells, with glycoxHDL having the greatest impact. Both the modified forms of HDL induced a significant increase in scavenger receptor expression and employed protein kinase C as well as extracellular signal-regulated kinase as downstream effectors of aldosterone release. Native HDL and modified HDL required Janus kinase-2 for combating increased demand in steroidogenesis. Therefore, our data support the hypothesis that diabetes-induced modification of HDL may promote adrenocortical aldosterone secretion via different signal transduction pathways. This significant influence on multiple signaling mechanisms could be targeted for future research to implement novel therapeutic trials. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1274-2 Authors Sarama Saha, Department of Internal Medicine III, Carl Gustav Carus Medical School, Technical University of Dresden, Fetscherstraße 74, 01307 Dresden, Germany Juergen Graessler, Department of Internal Medicine III, Carl Gustav Carus Medical School, Technical University of Dresden, Fetscherstraße 74, 01307 Dresden, Germany Peter E. H. Schwarz, Department of Internal Medicine III, Carl Gustav Carus Medical School, Technical University of Dresden, Fetscherstraße 74, 01307 Dresden, Germany Claudia Goettsch, Department of Internal Medicine III, Carl Gustav Carus Medical School, Technical University of Dresden, Fetscherstraße 74, 01307 Dresden, Germany Stefan R. Bornstein, Department of Internal Medicine III, Carl Gustav Carus Medical School, Technical University of Dresden, Fetscherstraße 74, 01307 Dresden, Germany Steffi Kopprasch, Department of Internal Medicine III, Carl Gustav Carus Medical School, Technical University of Dresden, Fetscherstraße 74, 01307 Dresden, Germany Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Tyrosinase is the key regulatory enzyme of melanogenesis and plays a major role in mammal coat color. For the first time, we have sequenced and characterized the tyrosinase (TYR) of Jining Gray Goat ( Capra hircus ), which is the world-famous fur-bearing animal with its special color and pattern. The full-length cDNA was cloned by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) method. As a result, one 2131-bp nucleotide sequence representing the full-length cDNA of TYR was obtained. The entire open reading frame (ORF) of the TYR is 1593 bp and encodes for 530 amino acids, which is well conserved compared with TYR of various species with higher degree of sequence similarity with other mammalian (74–99 %) than amphibian, aves, and fishes (56–73 %). The deduced amino acids contained one signal peptide, one transmembrane domain, five N-linked glycosylation sites, and two copper binding sites. The result of real-time quantitative PCR showed that the expression level of TYR was the highest in the dark-gray goats and the lowest in the light-gray ones, while the goats of dark-gray individuals have more than 50 % black fiber and light-gray ones less than 30 %. During the whole life of Jining gray goat, TYR expression level changes with certain regularity and their coat color will change correspondingly by investigating the expression level in ten development stages. After comparing the result and the coat phenotype, we presume that it seems to have a positive relationship between the color depth of coat and the expression level of TYR. Content Type Journal Article Pages 1-10 DOI 10.1007/s11010-012-1275-1 Authors Weiyun Chen, College of Animal Sciences and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China Hui Wang, College of Animal Sciences and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China Bin Dong, College of Animal Sciences and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China Zhongdian Dong, College of Animal Sciences and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China Fenna Zhou, College of Animal Sciences and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China Yong Fu, College of Animal Sciences and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China Yongqing Zeng, College of Animal Sciences and Technology, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    Pleiotropic effects of serotonin (5-HT) in the cardiovascular system are well documented. However, it remains to be elucidated, whether 5-HT is present in adult mammalian cardiomyocytes. To address this issue, we investigated the levels of 5-HT in blood, plasma, platelets, cardiac tissue, and cardiomyocytes from adult mice and for comparison in human right atrial tissue. Immunohistochemically, 5-HT was hardly found in mouse cardiac tissue, but small amounts could be detected in renal preparations, whereas adrenal preparations revealed a strong positive immunoreaction for 5-HT. Using a sensitive HPLC detection system, 5-HT was also detectable in the mouse heart and human atrium. Furthermore, we could identify 5-HT in isolated cardiomyocytes from adult mice. These findings were supported by detection of the activity of 5-HT-forming enzymes—tryptophan hydroxylase and aromatic l -amino acid decarboxylase—in isolated cardiomyocytes from adult mice and by inhibition of these enzymes with p -chlorophenylalanine and 3-hydroxybenzyl hydrazine. Addition of the first intermediate of 5-HT generation, that is 5-hydroxytryptophan, enhanced the 5-HT level and inhibition of monoamine oxidase by tranylcypromine further increased the level of 5-HT. Our findings reveal the presence and synthesis of 5-HT in cardiomyocytes of the mammalian heart implying that 5-HT may play an autocrine and/or paracrine role in the heart. Content Type Journal Article Pages 1-12 DOI 10.1007/s11010-012-1270-6 Authors Klaus Pönicke, Medical Faculty, Institute of Pharmacology and Toxicology, Martin-Luther-University Halle-Wittenberg, Magdeburger Str. 4, 06112 Halle (Saale), Germany Ulrich Gergs, Medical Faculty, Institute of Pharmacology and Toxicology, Martin-Luther-University Halle-Wittenberg, Magdeburger Str. 4, 06112 Halle (Saale), Germany Igor B. Buchwalow, Institut für Hämatopathologie, Fangdieckstraße 75a, 22547 Hamburg, Germany Steffen Hauptmann, Medical Faculty, Institute of Pathology, Martin-Luther-University Halle-Wittenberg, 06097 Halle (Saale), Germany Joachim Neumann, Medical Faculty, Institute of Pharmacology and Toxicology, Martin-Luther-University Halle-Wittenberg, Magdeburger Str. 4, 06112 Halle (Saale), Germany Journal Molecular and Cellular Biochemistry Online ISSN 1573-4919 Print ISSN 0300-8177

Beschreibung:    The kinetic principles of sorption of formaldehyde sorption on a polyfunctional weak-basic anion exchanger are considered. It is found that the limiting step of sorbate uptake is external diffusion. Parameters of formaldehyde sorption from aqueous solutions under dynamic conditions are determined. Content Type Journal Article Category Short Communications Pages 884-885 DOI 10.1134/S0036024412050354 Authors I. V. Voronyuk, Voronezh State University, Voronezh, Russia T. V. Eliseeva, Voronezh State University, Voronezh, Russia V. F. Selemenev, Voronezh State University, Voronezh, Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 5

Beschreibung:    The kinetics of tin oxidation was studied using Auger spectroscopy and characteristic electron energy loss spectroscopy. Studies were performed with continuous electron irradiation ( E p = 1800 eV) and without it depending on exposition in oxygen medium at a 10 −6 torr partial oxygen pressure and room temperature (maximum exposure in oxygen was 3000 Langmuir). Exposition to oxygen at 3000 L was shown to cause the formation of a continuous SnO 2 oxide layer, whereas electron irradiation with the same exposition stimulated the growth of a layer predominantly containing SnO. Content Type Journal Article Category Chemical Kinetics and Catalysis Pages 752-756 DOI 10.1134/S0036024412050032 Authors O. G. Ashkhotov, Kabardino-Balkar State University, ul. Chernyshevskogo 173, Nalchik, 360004 Russia I. B. Ashkhotova, Kabardino-Balkar State University, ul. Chernyshevskogo 173, Nalchik, 360004 Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 5

Beschreibung:    A 16-constant additive scheme was derived for calculating the physicochemical properties of saturated monoalcohols CH 4 O-C 9 H 20 O and decomposing the triangular numbers of the Pascal triangle based on the similarity of subgraphs in the molecular graphs (MGs) of the homologous series of these alcohols. It was shown, using this scheme for calculation of properties of saturated monoalcohols as an example, that each coefficient of the scheme (in other words, the number of methods to impose a chain of a definite length i 1 , i 2 , … on a molecular graph) is the result of the decomposition of the triangular numbers of the Pascal triangle. A linear dependence was found within the adopted classification of structural elements. Sixteen parameters of the schemes were recorded as linear combinations of 17 parameters. The enthalpies of vaporization L 298 K 0 of the saturated monoalcohols CH 4 O-C 9 H 20 O, for which there were no experimental data, were calculated. It was shown that the parameters are not chosen randomly when using the given procedure for constructing an additive scheme by decomposing the triangular numbers of the Pascal triangle. Content Type Journal Article Category Chemical Thermodynamics and Thermochemistry Pages 736-740 DOI 10.1134/S0036024412050123 Authors V. V. Grebeshkov, Tver State University, Tver, Russia V. M. Smolyakov, Tver State University, Tver, Russia Journal Russian Journal of Physical Chemistry A, Focus on Chemistry Online ISSN 1531-863X Print ISSN 0036-0244 Journal Volume Volume 86 Journal Issue Volume 86, Number 5