Publication Date:
2019
Description:
〈h3〉Abstract〈/h3〉
〈span〉
〈h3〉Aims〈/h3〉
〈p〉This study defines key genes involved in Fe homeostasis and reveals cultivar differences in Fe deficiency responses in peanuts.〈/p〉
〈/span〉
〈span〉
〈h3〉Methods〈/h3〉
〈p〉A comparative transcriptome analysis was conducted on two peanut cultivars, Silihong and Fenghua 1, under Fe-sufficient and -deficient conditions. Plant growth and metal contents were measured.〈/p〉
〈/span〉
〈span〉
〈h3〉Results〈/h3〉
〈p〉Silihong is more tolerant to Fe-deficiency than Fenghua 1. Fe deficiency increased the uptake of Zn, Mn, Mg, Ca and Cu in peanut plants. A total of 56 and 19 transporter genes were identified to be differentially expressed genes in Silihong and Fenghua 1 respectively. Two cultivars shared twelve common differentially expressed genes encoding OPT3, FRO2, Nramp3, YSL3, IRT1, FER3 and FRO7. Silihong showed higher expressions of 〈em〉AHA4〈/em〉, 〈em〉COPT1〈/em〉, 〈em〉FRO2〈/em〉, 〈em〉IRT1〈/em〉, 〈em〉MTP4〈/em〉, 〈em〉MTP9〈/em〉, 〈em〉Nramp5〈/em〉, 〈em〉OPT3〈/em〉 and 〈em〉VITH4〈/em〉, while Fenghua 1 exhibited higher expressions of 〈em〉ATX1〈/em〉, 〈em〉HMA5〈/em〉, 〈em〉MPT1〈/em〉, 〈em〉OPT6〈/em〉, 〈em〉YSL3〈/em〉, 〈em〉YSL7〈/em〉, 〈em〉ZIP1〈/em〉, 〈em〉ZIP4〈/em〉, 〈em〉ZIP5〈/em〉 and 〈em〉ZIP11〈/em〉 under Fe deficiency.〈/p〉
〈/span〉
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〈h3〉Conclusions〈/h3〉
〈p〉Peanuts overcome Fe deficiency by increasing Fe uptake and altering its transport pathway, in which 〈em〉FRO2〈/em〉, 〈em〉IRT1〈/em〉, 〈em〉Nramp3〈/em〉, 〈em〉YSL3〈/em〉 and 〈em〉OPT3〈/em〉 are involved. Higher expression of 〈em〉AHA4〈/em〉, 〈em〉FRO2〈/em〉, 〈em〉IRT1〈/em〉 and 〈em〉Nramp5〈/em〉 might be responsible for higher Fe-deficiency tolerance in Silihong. Induction of 〈em〉IRT1〈/em〉, 〈em〉Nramp5〈/em〉 and 〈em〉COPT1〈/em〉 by Fe deficiency contribute to increased metal uptake in peanut plants.〈/p〉
〈/span〉
Print ISSN:
0032-079X
Electronic ISSN:
1573-5036
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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