Publication Date:
2011-06-15
Description:
This study aims to investigate the colonization of poplar by the endophyte Pseudomonas putida W619 and its capacity to promote plant growth. Poplar cuttings were inoculated with P. putida W619 (wild-type or gfp -labelled). The colonization of both strains was investigated and morphological, physiological and biochemical parameters were analyzed to evaluate plant growth promotion. Inoculation with P. putida W619 (wild-type) resulted in remarkable growth promotion, decreased activities of antioxidative defence related enzymes, and reduced stomatal resistance, all indicative of improved plant health and growth in comparison with the non-inoculated cuttings. In contrast, inoculation with gfp -labelled P. putida W619 did not promote growth; it even had a negative effect on plant health and growth. Furthermore, compared to the wildtype strain, colonization by the gfp -labelled P. putida W619:: gfp 1 was much lower; it only colonized the rhizosphere and root cortex while the wild-type strain also colonized the root xylem vessels. Despite the strong plant growth promoting capacity of P. putida W619 (wild-type), after gfp labelling its growth promoting characteristics disappeared and its colonization capacity was strongly influenced; for these reasons gfp labelling should be applied with sufficient caution. Content Type Journal Article Pages 1-14 DOI 10.1007/s11104-011-0831-x Authors Nele Weyens, Environmental Biology, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium Jana Boulet, Environmental Biology, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium Dirk Adriaensen, Laboratory of Cell Biology & Histology, Department of Veterinary Sciences, Antwerp University, Groenenborgerlaan 171, 2020 Antwerp, Belgium Jean-Pierre Timmermans, Laboratory of Cell Biology & Histology, Department of Veterinary Sciences, Antwerp University, Groenenborgerlaan 171, 2020 Antwerp, Belgium Els Prinsen, Department of Biology, Groenenborgercampus, Antwerp University, Groenenborgerlaan 171, 2020 Antwerp, Belgium Sandra Van Oevelen, Department of Biology, Groenenborgercampus, Antwerp University, Groenenborgerlaan 171, 2020 Antwerp, Belgium Jan D’Haen, Institute for Materials Research, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium Karen Smeets, Biodiversity, Phylogeny & Population Studies, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium Daniel van der Lelie, Research Triangle Institute (RTI), International 3040 Cornwallis Road, Research Triangle Park, NC 27709-2194, USA Safiyh Taghavi, Brookhaven National Laboratory (BNL), Biology Department, Building 463, Upton, NY 11973, USA Jaco Vangronsveld, Environmental Biology, Hasselt University, Agoralaan Building D, B-3590 Diepenbeek, Belgium Journal Plant and Soil Online ISSN 1573-5036 Print ISSN 0032-079X
Print ISSN:
0032-079X
Electronic ISSN:
1573-5036
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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