ALBERT

Description: The objective of this study is to understand which requirements for cereal cultivars—with regard to climate change adaptation—are in demand by farmers and advisors, and to clarify whether there are any differences in their assessments. A comparative survey was used to collect data from 410 farmers and 114 advisors in Germany. The majority of both farmers and advisors reported perceivable effects of climatic change on plant production. The increase in droughts and hot spells, the increased incidence of torrential rain, and mild winters were mentioned as the main effects of climate change. For climate change adaptation, the farmers and advisors mostly relied on a locally-adapted cultivar selection. It is estimated that eco-stability, grain yield, resistance to lodging and drought tolerance are important cultivar properties. In the study, farmers and advisors equally pointed out the need for additional cultivar evaluation according to eco-stability. Finally, only minor differences regarding farmers’ and advisors’ assessments were found within the study. The outcome of this research points to the need of implementing farmers’ demands in cultivar recommendations. For example, an impartial assessment of cultivars’ eco-stability could help support the choice of cultivars and reduce the growing risks in cereal production with regard to climate change.

Description: The mobilization of nutrients from fish sludge (i.e., feces and uneaten feed) plays a key role in optimizing the resource utilization and thus in improving the sustainability of aquaponic systems. While several studies have documented the aerobic and anaerobic digestion performance of aquaculture sludge, the impact of the digestate on plant growth has yet to be understood. The present study examines the impact of either an aerobic or an anaerobic digestion effluent on lettuce plant growth, by enriching a mixture of aquaculture and tap water with supernatants from both aerobic and anaerobic batch reactors. The lettuce plants grown in the hydroponic system supplied with supernatant from an anaerobic reactor had significantly better performance with respect to weight gain than both, those in the system where supernatant from the aerobic reactor was added, as well as the control system. It can be hypothesized that this effect was caused by the presence of NH4+ as well as dissolved organic matter, plant growth promoting rhizobacteria and fungi, and humic acid, which are predominantly present in anaerobic effluents. This study should therefore be of value to researchers and practitioners wishing to further develop sludge remineralization in aquaponic systems.

Description: Arbuscular mycorrhizal fungi (AMF) play a major role in the uptake of nutrients by agricultural plants. Nevertheless, some agricultural practices can interrupt fungal-plant signaling and thus impede the establishment of the mycorrhizal symbiosis. A field experiment performed over a 5-year period demonstrated that both the absence of tillage and of nitrogen (N) fertilization improved AMF colonization of wheat roots. Moreover, under no-till conditions, N uptake and aboveground biomass production did not vary significantly between N-fertilized and N-unfertilized plots. In contrast, both N uptake and above ground biomass were much lower when N fertilizer was not added during conventional tillage. This finding strongly suggests that for wheat, no-till farming is a sustainable agricultural system that allows a gradual reduction in N fertilizer use by promoting AMF functionality and at the same time increasing N uptake.

Description: Foliar fertilization, or the application of nutrient solutions to the foliage of plants, has become a very important tool as a supplement to traditional soil fertilization. So far, knowledge about the real mechanisms of foliar nutrient uptake is still limited. In this study different manganese (Mn) and zinc (Zn) carriers differing in their solubility and chemical characteristics (chelated or non-chelated, with or without the presence of a surfactant-penetrant) were compared with regard to their penetration characteristics through enzymatically-isolated cuticles. The experiments were explicitly conducted under high humidity conditions in order not to penalize compounds with a higher deliquescent point. The results show that Mn penetrates more rapidly through the cuticle than Zn ions for unknown reasons. The addition of a surfactant-penetrant enhances the penetration rate in the case of Mn ions. This trend is much less pronounced for zinc ions. Formulations based on insoluble carriers, such as carbonate or oxide, only poorly penetrate through the cuticle. In order to rapidly control micronutrient deficiency problems, only fully water soluble micronutrient carriers should be used.

Description: Nitrogen (N) losses negatively impact groundwater quality. Spring wheat genotypes varying in N-fertilizer recovery were studied (by using lysimeters) for their potential to minimize NO3-N leaching during spring and summer, over a three-year period. Additionally, we examined to what extent root growth and NO3-N leaching explain the well-known difference found between apparent and isotopic N recovery. The genotypes were grown under low (2 g m−2) and high (27 g m−2) N fertilizer supply. On average, the apparent and isotopic recoveries of N fertilizer by wheat were 43% and 51%, respectively. The three genotypes varied in fertilizer N recovery but not in NO3-N leaching, which only accounted for 15% of the applied N fertilizer. The differences in N uptake, fertilizer N recovery and root growth among the genotypes were not associated with the leached NO3-N because root growth and N uptake were not well synchronized with NO3-N leaching. Already at stem elongation 70% to 98% of the season-long NO3-N leaching had already taken place. Thus, the ability to minimize in-season NO3-N leaching by using spring wheat genotypes with higher fertilizer N recovery was limited because maximum N leaching occurred in the early crop season.

Description: Agronomic N-use efficiency is the basis for economic and environmental efficiency, and an effective agro-ecosystem management practice, improving nutrient use efficiency, is a crucial challenge for a more sustainable production of horticultural, industrial and cereal crops. However, discrepancy between theory and practice still exists, coming from large gaps in knowledge on net-N immobilization/mineralization rates in agro-ecosystems, as well as on the effects of indigenous and applied N to crop response. A more thorough understanding of these topics is essential to improve N management in agricultural systems. To this end, the present Special Issue collects research findings dealing with different aspects of agronomic efficiency of N in different agro-ecosystems, and environmental impact derived from fertilization management practices. In particular, the Special Issue contains selected papers, which concern a wide range of topics, including analyzing tools, options of management, calculation equation and modeling approaches.

Description: A two-year field study was conducted to investigate the performance of a lightweight trash-board moldboard plow (with and without a trash-board), as influenced by stubble height and water content. Both fields were measured for the performance of a trash-board moldboard plow when used during the optimization of the plowing depth, the water content, and the reaction forces. The results showed that in the first year, when a trash-board was required, the results were significantly different. The fields had lower draft and reaction force in the soil with only stubble height, which was greater than that in the soil with dense straw for all water content levels. This was also observed in the second year for the whole depth. This study shows that the moldboard plow with a trash-board provided minimum draft and reaction forces with only straw and heavy straw. The results indicate that straw nearby shear significantly increased displacement for all treatments, with variance of straw nearby moldboard. Hence, the results verify that a trash-board continuously created large soil fragmentation with different water content. Straw labels create a position of straw which also allows for better results. It is important to install trash-boards with the moldboard plow for heavy straw incorporation.

Description: In order to standardize the quality of agricultural products, it is necessary to control the factors affecting plant development, such as plant nutrition. The best results in terms of homogeneity of the quality of vegetable crops were achieved using inert substrates and application of nutrients; however, production costs are high due to the cost of irrigation systems and substrate management and importation. This work aims to evaluate the effect of the local substrate mix and the amount of organic fertilizer on different quality parameters of coriander. To evaluate the quality of coriander, we considered different parameters such as size, biomass, antioxidant capacity and aroma (evaluated by volatile compounds detection with gas chromatography). The results show that the culture system differentially affects each parameter, and the compounds associated with the aroma of coriander and the diameter of plants are sensitive to the culture system, while the length of plants, number of leaves and antioxidant activity are not affected by the concentration of fertilizer. Moreover, organic farming conditions do not reduce quality parameters of the crops when using adequate fertilization. Additionally, local substrates would be practical substitutes for expensive importations.

Description: In intensive agriculture, N supply often exceeds crop requirements, even in nitrate vulnerable zones (NVZ). In farmland, the N surplus gives rise to NO3− leaching and consequent groundwater pollution. The present study aimed at proposing measures to reduce N leaching and hence improve N efficiency in a buffalo livestock farm located in the NVZ of Latina plain (Central Italy). The farm was cultivated with forage crops in a double annual crop rotation: Italian ryegrass (Lolium multiflorum Lam.) in winter and silage corn (Zea mays L.) in summer. Mineral and organic fertilizers were supplied to both crops. The annual N budget and soil solution NO3-N concentrations were evaluated using a modeling approach. The performance of the WinEPIC model in simulating the response of the NO3-N concentration in percolation to the N application rate was assessed and validated by field measurements of the NO3-N concentration in the soil solution. Three scenarios were proposed to identify the best practice to minimize the environmental impact of N application without significant yield loss. Also, recommendations of best practices in N fertilization and animal manure spreading were given. This study thus provides useful preliminary information for decision-making in agriculture/environmental policies.

Description: Genotypic variation in N efficiency defined as high grain yield under limited nitrogen (N) supply of winter oilseed-rape line-cultivars has been predominantly attributed to N uptake efficiency (NUPT) through maintained N uptake during reproductive growth related to functional stay-green. For investigating the role of stay-green, N retranslocation and N uptake during the reproductive phase for grain yield formation, two line cultivars differing in N starvation-induced leaf senescence were grown in a field experiment without mineral N (N0) and with 160 kg N·ha−1 (N160). Through frequent harvests from full flowering until maturity N uptake, N utilization and apparent N remobilization from vegetative plant parts to the pods could be calculated. NUPT proved being more important than N utilization efficiency (NUE) for grain yield formation under N-limiting (N0) conditions. For cultivar differences in N efficiency, particularly N uptake during flowering (NUPT) and biomass allocation efficiency (HI) to the grains, were decisive. Both crop traits were related to delayed senescence of the older leaves. Remobilization of N particularly from stems and leaves was more important for pod N accumulation than N uptake after full flowering. Pod walls (high N concentrations) and stems (high biomass) mainly contributed to the crop-residue N at maturity. Decreasing the crop-inherent high N budget surplus of winter oilseed-rape requires increasing the low N remobilization efficiency particularly of pod-wall N to the grains. Addressing this conclusion, multi-year and -location field experiments with an extended range of cultivars including hybrids are desirable.

Description: Stripe rust (caused by Puccinia striiformis f. sp. tritici) of wheat (Triticum aestivum) is a devastating disease in temperate regions when susceptible varieties are grown and environmental conditions sustain high disease pressures. With frequent and severe outbreaks, disease resistance is a key tool for controlling stripe rust on wheat. The goal of this research was to identify quantitative trait loci (QTL) involved in stripe rust resistance from the important US Pacific Northwest soft white winter wheat varieties “Eltan” and “Finch”. An F2:5 recombinant inbred line (RIL) mapping population of 151 individuals derived from the Finch × Eltan cross was developed through single seed descent. A linkage map comprising 683 unique single nucleotide polymorphism (SNP) loci and 70 SSR markers were used to develop 22 linkage groups consisting of 16 of the 21 chromosomes. Stripe rust data were collected on the RILs during the summers of 2012 to 2014. QTL analysis identified two genomic regions on chromosomes 4A (QYrel.wak-4A) and 6B (QYrfi.wak-6B) associated with resistance from Eltan and Finch, respectively. The results of the QTL analysis showed that QYrel.wak-4A and QYrfi.wak-6B reduced infection type and disease severity. Based upon both molecular and phenotypic differences, QYrel.wak-4A is a novel QTL for adult plant resistance (APR) to stripe rust.

Description: Ascochyta blight (AB) caused by Ascochyta rabiei (Pass.) Labr. is an important and widespread disease of chickpea (Cicer arietinum L.) worldwide. The disease is particularly severe under cool and humid weather conditions. Breeding for host resistance is an efficient means to combat this disease. In this paper, attempts have been made to summarize the progress made in identifying resistance sources, genetics and breeding for resistance, and genetic variation among the pathogen population. The search for resistance to AB in chickpea germplasm, breeding lines and land races using various screening methods has been updated. Importance of the genotype × environment (GE) interaction in elucidating the aggressiveness among isolates from different locations and the identification of pathotypes and stable sources of resistance have also been discussed. Current and modern breeding programs for AB resistance based on crossing resistant/multiple resistant and high-yielding cultivars, stability of the breeding lines through multi-location testing and molecular marker-assisted selection method have been discussed. Gene pyramiding and the use of resistant genes present in wild relatives can be useful methods in the future. Identification of additional sources of resistance genes, good characterization of the host–pathogen system, and identification of molecular markers linked to resistance genes are suggested as the key areas for future study.

Description: Field experiments at Oakes, ND, USA in 2010 and Carrington, ND, USA in 2011 were conducted to evaluate the potential for cover crops grown in the Northern Great Plains, USA in order to reduce weed emergence and density in irrigated potatoes. Treatments included five cover crop treatments and three cover crop termination treatments. Termination of cover crops was done with glyphosate, disk-till, and roto-till. Cover crop biomass accumulation was greatest for rye/canola and triticale at Oakes, and hairy vetch and hairy vetch/rye at Carrington. Cover crop and termination affected weed control 14, 29, and 51 days after planting (DAP) at Oakes. Weed control at Carrington was at least 90% for all cover crop and termination treatments at all three evaluation timings. Marketable yield at Oakes was greater when roto-till was used to terminate the cover crops compared with disk-till or herbicide, which is beneficial for organic systems where herbicides are not used. Marketable yield at Carrington was not affected by cover crop or termination treatments. Results suggest that cover crops can successfully be integrated into irrigated potato production for weed control with yields equal to no cover crop, and with attention to potential mechanical difficulties.

Description: Soil and water pollution by metals and other toxic chemicals is difficult to measure and control, and, as such, presents an ongoing global threat to sustainable agriculture and human health. Efforts to remove contaminants by plant-mediated pathways, or “phytoremediation”, though widely studied, have failed to yield consistent, predictable removal of biological and chemical contaminants. Emerging research has revealed that one major limitation to using plants to clean up the environment is that plants are programmed to protect themselves: Like white blood cells in animals, border cells released from plant root tips carry out an extracellular trapping process to neutralize threats and prevent injury to the host. Variability in border cell trapping has been found to be correlated with variation in sensitivity of roots to aluminum, and removal of border cell results in increased Al uptake into the root tip. Studies now have implicated border cells in responses of diverse plant roots to a range of heavy metals, including arsenic, copper, cadmium, lead, mercury, iron, and zinc. A better understanding of border cell extracellular traps and their role in preventing toxin uptake may facilitate efforts to use plants as a nondestructive approach to neutralize environmental threats.

Description: A decrease in water resources around the globe in irrigated agriculture has resulted in a steep decline in irrigation water availability. Therefore, management options for efficient use of available irrigation water are inevitable. Deciding the critical time, frequency and amount of irrigation are compulsory to achieve higher crop outputs. Hence, this two-year field study was conducted to assess the role of different row spacings, i.e., 20, 25 and 30 cm, on growth, productivity, and water use efficiency (WUE) of wheat under deficit supplemental irrigation (DSI) at the vegetative and reproductive phase by using surplus supplemental irrigation (SSI) throughout the growing season as the control. DSI at both growth stages, and the reproductive stage in particular, changed the crop allometry, yield and net income of wheat. However, narrow spacing (20 cm) resulted in efficient use of available irrigation water (DSI and SSI) with higher yield, WUE and economic returns. Interestingly, wider spacing resulted in a higher number of grains per spike with higher 1000-grain weight under SSI and DSI, but final yield output remained poor due to a lower number of productive tillers. It was concluded that reducing irrigation during the vegetative stage is less damaging compared with the reproductive phase; therefore, sufficient supplemental irrigation must be added at the reproductive stage, particularly during grain-filling. Further, narrow spacing (20 cm) resulted in efficient utilization of available irrigation water; therefore, wheat must be grown at a narrow spacing to ensure the efficient utilization of available irrigation water.

Description: In recent years, Archaea have, with increasing frequency, been found to colonize both agricultural and forest soils in temperate and boreal regions. The as yet uncultured group I.1c of the Thaumarchaeota has been of special interest. These Archaea are widely distributed in mature vegetated acidic soils, but little has been revealed of their physiological and biological characteristics. The I.1c Thaumarchaeota have been recognized as a microbial group influenced by plant roots and mycorrhizal fungi, but appear to have distinct features from their more common soil dwelling counterparts, such as the Nitrosotalea or Nitrososphaera. They appear to be highly dependent on soil pH, thriving in undisturbed vegetated soils with a pH of 5 or below. Research indicate that these Archaea require organic carbon and nitrogen sources for growth and that they may live both aerobically and anaerobically. Nevertheless, pure cultures of these microorganisms have not yet been obtained. This review will focus on what is known to date about the uncultured group I.1c Thaumarchaeota formerly known as the “Finnish Forest Soil” (FFS) Archaea.

Description: Tomatoes (Lycopersicon esculentum Mill) are one of the biggest vegetable crops in the world, supplying a wide range of vitamins, minerals and fibre in human diets. In the tropics, tomatoes are predominantly grown under sub-optimal conditions by subsistence farmers, with exposure to biotic and abiotic stresses in the open field. Whitefly (Bemisia tabaci Gennadius) is one of the major pests of the tomato, potentially causing up to 100% yield loss. To control whitefly, most growers indiscriminately use synthetic insecticides which negatively impact the environment, humans, and other natural pest management systems, while also increasing cost of production. This study sought to investigate the effectiveness of agronet covers and companion planting with aromatic basil (Ocimum basilicum L.) as an alternative management strategy for whitefly in tomatoes and to evaluate the use of these treatments ontomato growth and yield. Two trials were conducted at the Horticulture Research and Training Field, Egerton University, Njoro, Kenya. Treatments comprised a combination of two factors, (1) growing environment (agronet and no agronet) and (2) companion planting with a row of basil surrounding tomato plants, a row of basil in between adjacent rows of tomato, no companion planting. Agronet covers and companion cropping with a row of basil planted between adjacent tomato rows significantly lowered B. tabaci infestation in tomatoes by 68.7%. Better tomato yields were also recorded in treatments where the two treatments were used in combination. Higher yield (13.75 t/ha) was obtained from tomatoes grown under agronet cover with a basil row planted in between adjacent rows of the tomato crop compared to 5.9 t/ha in the control. Non-marketable yield was also lowered to5.9 t/ha compared to 9.8 t/ha in the control following the use of the two treatments in combination. The results of this study demonstrate the potential viability of using companion cropping and agronet covers in integrated management of B. tabaci and improvement of tomato yield.

Description: The doubling of the world’s agricultural production for the past four decades has been associated with a seven-fold increase in nitrogen (N) fertilization [1] which has caused major detrimental impacts onthediversityandfunctioningofthenon-agriculturalbacterial,animalandplantecosystems,notably through the process of freshwater and marine ecosystem eutrophication [2].[...]

Description: Crop system models are generally parametrized with daily air temperatures recorded at 1.5 or 2 m height. These data are not able to represent temperatures at the canopy level, which control crop growth, and the impact of heat stress on crop yield, which are modified by canopy characteristics and plant physiological processes Since such data are often not available and current simulation approaches are complex and/or based on unrealistic assumptions, new methods for integrating canopy temperatures in the framework of crop system models are needed. Based on a forward stepwise-based model selection procedure and quantile regression analyses, we developed empirical regression models to predict winter wheat canopy temperatures obtained from thermal infrared observations performed during four growing seasons for three irrigation levels. We used daily meteorological variables and the daily output data of a crop system model as covariates. The standard cross validation revealed a root mean square error (RMSE) of ~0.8 °C, 1.5–2 °C and 0.8–1.2 °C for estimating mean, maximum and minimum canopy temperature, respectively. Canopy temperature of both water-deficit and fully irrigated wheat plots significantly differed from air temperature. We suggest using locally calibrated empirical regression models of canopy temperature as a simple approach for including potentially amplifying or mitigating microclimatic effects on plant response to temperature stress in crop system models.

Description: Enhanced efficiency nitrogen (N) fertilizers (EEFs) may improve crop recovery of fertilizer-N, but there is evidence that some EEFs cause a lag in crop growth compared to growth with standard urea. Biomass and mineral nutrient accumulation was investigated in rice fertilized with urea, urea-3,4-dimethylpyrazole phosphate (DMPP) and polymer-coated urea (PCU) to determine whether any delays in biomass production alter the accumulation patterns, and subsequent grain concentrations, of key mineral nutrients. Plant growth and mineral accumulation and partitioning to grains did not differ significantly between plants fertilized with urea or urea-DMPP. In contrast, biomass accumulation and the accumulation of phosphorus, potassium, calcium, magnesium, copper, zinc and manganese were delayed during the early growth phase of plants fertilized with PCU. However, plants in the PCU treatment ultimately compensated for this by increasing growth and nutrient uptake during the latter vegetative stages so that no differences in biomass or nutrient accumulation generally existed among N fertilizer treatments at anthesis. Delayed biomass accumulation in rice fertilized with PCU does not appear to reduce the total accumulation of mineral nutrients, nor to have any impact on grain mineral nutrition when biomass and grain yields are equal to those of rice grown with urea or urea-DMPP.

Description: The editors of agronomy would like to express their sincere gratitude to the following reviewers for assessing manuscripts in 2015. [...]

Description: Sugarcane, Saccharum spp., in the United States is attacked by a number of different arthropod pests. The most serious among those pests are two stalk boring moths in the Family Crambidae: the sugarcane borer, Diatraea saccharalis (F.), and the Mexican rice borer, Eoreuma loftini (Dyar). The two species are affected by abiotic and biotic environmental stress factors. Water deficit and excessive soil nitrogen alter physical and physiochemical aspects of the sugarcane plant that make the crop increasingly vulnerable to E. loftini. Weed growth can be competitive with sugarcane but it also supports enhanced abundances and diversity of natural enemies that can suppress infestations of D. saccharalis. In an instance where the stalk borer is considered a stress factor, proximity of vulnerable crops to sugarcane can influence levels of E. loftini infestation of sugarcane. The adverse effects of each stress factor, in terms of stalk borer attack, can be reduced by adopting appropriate cultural practices, such as adequate irrigation, judicious use of nitrogen fertilizer, using noncompetitive weed growth, and not planting vulnerable crops near sugarcane fields. Understanding the relationships between stress factors and crop pests can provide valuable insights for plant breeders and tools for incorporation into integrated pest management strategies.

Description: Barley is a cereal of primary importance for forage and human nutrition, and is a useful model for wheat. Autophagy genes first described in yeast have been subsequently isolated in mammals and Arabidopsis thaliana. In Arabidopsis and maize it was recently shown that autophagy machinery participates in nitrogen remobilization for grain filling. In rice, autophagy is also important for nitrogen recycling at the vegetative stage. In this study, HvATGs, HvNBR1 and HvATI1 sequences were identified from bacterial artificial chromosome (BAC), complementary DNA (cDNA) and expressed sequence tag (EST) libraries. The gene models were subsequently determined from alignments between genome and transcript sequences. Essential amino acids were identified from the protein sequences in order to estimate their functionality. A total of twenty-four barley HvATG genes, one HvNBR1 gene and one HvATI1 gene were identified. Except for HvATG5, all the genomic sequences found completely matched their cDNA sequences. The HvATG5 gene sequence presents a gap that cannot be sequenced due to its high GC content. The HvATG5 coding DNA sequence (CDS), when over-expressed in the Arabidopsis atg5 mutant, complemented the plant phenotype. The HvATG transcript levels were increased globally by leaf senescence, nitrogen starvation and dark-treatment. The induction of HvATG5 during senescence was mainly observed in the flag leaves, while it remained surprisingly stable in the seedling leaves, irrespective of the leaf age during stress treatment.

Description: Rhizodeposition is considered a primary reason for the plant identity effect. However, the detection of distinct rhizosphere bacterial communities (RBC) with different plant species has been variable. The aim of this study was to examine the potential explanations for this variability using three perennial grassland species. In a Kansas field experiment, over two growing seasons, we sampled RBC during the active growth and flowering stages of Agrostis gigantea, Andropogon gerardii and Helianthus maximiliani to: (1) determine the extent of the plant identity effect among these species and if the effect was maintained over time; (2) assess if RBC showed seasonal patterns, corresponding to plant phenology; and (3) examine if soil properties were important for structuring these communities. We found that Helianthus RBC were distinct from those of Agrostis and Andropogon only when Helianthus was flowering. Further, Helianthus RBC exhibited seasonal shifts corresponding to plant phenology. In contrast, Agrostis and Andropogon RBC were similar over time and exhibited gradual non-seasonal changes in compositions. Similar results were observed when accounting for soil properties. Overall, the observance of a plant identity effect depended on the plant species and when RBC were sampled. The seasonality of RBC also depended on the plant species examined.

Description: Soybean is the most produced and consumed oil seed crop worldwide. In 2013, 226 million metric tons were produced in over 70 countries. Organically produced soybean represents less than 0.1% of total world production. In the USA, the certified organic soybean crop was grown on 53 thousand ha or 0.17% of the total soybean acreage in the USA (32 million ha) in 2011. A gradual increase in production of organically grown soybean has occurred since the inception of organic labeling due to increased human consumption of soy products and increased demand for organic soybean meal to produce organic animal products. Production constraints caused by pathogens and insect pests are often similar in organic and non-organic soybean production, but management between the two systems often differs. In general, the non-organic, grain-type soybean crop are genetically modified higher-yielding cultivars, often with disease and pest resistance, and are grown with the use of synthetic pesticides. The higher value of organically produced soybean makes production of the crop an attractive option to some farmers. This article reviews production and uses of organically grown soybean in the USA, potential constraints to production caused by pathogens and insect pests, and management practices used to reduce the impact of these constraints.

Description: About 1% of New Zealand farmland is managed organically. Nitrogen is the nutrient most likely to limit organic crop production. A potential solution is incorporation of compost to supply N. About 726,000 t of municipal garden and kitchen wastes are sent to landfills annually. Composting offers a means of reducing the impact of landfill wastes on the wider environment. Organically certified compost (N content typically 2% to 2.5%) is available from some municipal composting plants. To be effectively used on organic farms, the rate of N release (mineralization) must be known. Laboratory incubations were conducted to quantify mineralization of compost N under controlled (temperature and moisture) conditions. Nitrogen availability and crop yields from a one-off application of compost (25–100 t·ha−1) were also assessed in two field trials (using cereal and forage crops). The results suggested that a relatively small part (13%–23%) of compost N was used by the crops in 3–4 years. Much of this was mineral N present at the time of application. Mineralization rates in the laboratory and field studies were much lower than expected from published work or compost C:N ratio (considered an important indicator of N mineralization potential of composts).

Description: Sugarcane breeding is very difficult and it takes 12 to 14 years to develop a new cultivar for commercial production. This is because sugarcane varieties are highly polyploid, inter-specific hybrids with 100 to 130 chromosomes that may vary across geographical areas. Other obstacles/constraints include the small size of flowers that may not synchronize but may self-pollinate, difficulty in distinguishing hybrids from self progenies, extreme (G × E) interactive effect, and potential variety mis-identification during vegetative propagation and varietal exchange. To help cane breeders circumvent these constraints, a simple sequence repeats (SSR)-based molecular identity database has been developed at the United States Department of Agriculture-Agricultural Research Service, Sugarcane Research Unit in Houma, LA. Since 2005, approximately 2000 molecular identities have been constructed for clones of sugarcane and related Saccharum species that cover geographical areas including Argentina, Australia, Bangladesh, China, Colombia, India, Mexico, Pakistan, South Africa, Thailand, USA (Louisiana, Florida, Texas, and Hawaii), and Venezuela. The molecular identity database is updated annually and has been utilized to: (1) provide molecular descriptors to newly registered cultivars; (2) identify in a timely fashion any mislabeled or unidentifiable clones from cross parents and field evaluation plots; (3) develop de novo clones of energy cane with S. spontaneum cytoplasm; (4) provide clone-specific fingerprint information for assessing cross quality and paternity of polycross; (5) determine genetic relatedness of parental clones; (6) select F1 hybrids from (elite × wild) or (wild × elite) crosses; and (7) investigate the inheritance of SSR markers in sugarcane. The integration of the molecular identity database into the sugarcane breeding program may improve the overall efficacy of cultivar development and commercialization.

Description: Rhizoremediation is a bioremediation technique whereby microbial degradation of organic contaminants occurs in the rhizosphere. It is considered to be an effective and affordable “green technology” for remediating soils contaminated with petroleum hydrocarbons. Root exudation of a wide variety of compounds (organic, amino and fatty acids, carbohydrates, vitamins, nucleotides, phenolic compounds, polysaccharides and proteins) provide better nutrient uptake for the rhizosphere microbiome. It is thought to be one of the predominant drivers of microbial communities in the rhizosphere and is therefore a potential key factor behind enhanced hydrocarbon biodegradation. Many of the genes responsible for bacterial adaptation in contaminated soil and the plant rhizosphere are carried by conjugative plasmids and transferred among bacteria. Because root exudates can stimulate gene transfer, conjugation in the rhizosphere is higher than in bulk soil. A better understanding of these phenomena could thus inform the development of techniques to manipulate the rhizosphere microbiome in ways that improve hydrocarbon bioremediation.

Description: The NRT2 transporters mediate High Affinity Nitrate/NitriteTransport (HAN/NiT), which are essential for nitrogen acquisition from these inorganic forms. The NRT2 proteins are encoded by a multigene family in plants, and contain 12 transmembrane-spanning domains. Chlamydomonas reinhardtii has six NRT2, two of which (NRT2.5 and NRT2.4) are located in Chromosome III, in tandem head to tail. cDNAs for these genes were isolated and their sequence revealed that they correspond to half-size NRT2 transporters each containing six transmembrane domains. NRT2.5 has long N- and C- termini sequences without known homology. NRT2.4 also contains long termini sequences but smaller than NRT2.5. Expression of both studied genes occurred at a very low level, slightly in darkness, and was not modified by the N or C source. Silencing of NRT2.4 by specific artificial miRNA resulted in the inhibition of nitrite transport in the absence of other HANNiT (NRT2.1/NAR2) in the cell genetic background. Nitrite transport activity in the Hansenula polymorpha Δynt::URA3 Leu2 mutant was restored by expressing CrNRT2.4. These results indicate that half-size NRT2 transporters are present in photosynthetic organisms and that NRT2.4 is a HANiT.

Description: Cellulosic biofuel production is expected to increase in the US, and the targeted establishment of biofuel agriculture in marginal lands would reduce competition between biofuels and food crops. While poorly drained, seasonally saturated lowland landscape positions are marginal for production of row crops and switchgrass (Panicum virgatum L.), it is unclear whether species-diverse tallgrass prairie yield would suffer similarly in saturated lowlands. Prairie yields typically increase as graminoids become more dominant, but it is uncertain whether this trend is due to greater aboveground net primary productivity (ANPP) or higher harvest efficiency in graminoids compared to forbs. Belowground biomass, a factor that is important to ecosystem service provisioning, is reduced when switchgrass is grown in saturated lowlands, but it is not known whether the same is true in species-diverse prairie. Our objectives were to assess the effect of topography on yields and live belowground biomass in row crops and prairie, and to determine the mechanisms by which relative graminoid abundance influences tallgrass prairie yield. We measured yield, harvest efficiency, and live belowground biomass in upland and lowland landscape positions within maize silage (Zea mays L.), winter wheat (Triticum aestivum L.), and restored tallgrass prairie. Maize and winter wheat yields were reduced by more than 60% in poorly drained lowlands relative to well-drained uplands, but diverse prairie yields were equivalent in both topographic settings. Prairie yields increased by approximately 45% as the relative abundance of graminoids increased from 5% to 95%. However, this trend was due to higher harvest efficiency of graminoids rather than greater ANPP compared to forbs. In both row crops and prairie, live belowground biomass was similar between upland and lowland locations, indicating consistent biomass nutrient sequestration potential and soil organic matter inputs between topographic positions. While poorly drained, lowland landscape positions are marginal lands for row crops, they appear prime for the cultivation of species-diverse tallgrass prairie for cellulosic biofuel.

Description: High air temperatures during the crop growing season can reduce harvestable yields in major agronomic crops worldwide. Repeated and prolonged high night air temperature stress may compromise plant growth and yield. Crop varieties with improved heat tolerance traits as well as crop management strategies at the farm scale are thus needed for climate change mitigation. Crop yield is especially sensitive to night-time warming trends. Current studies are mostly directed to the elevated night-time air temperature and its impact on crop growth and yield, but less attention is given to the understanding of night-time soil temperature management. Delivering irrigation water through drip early evening may reduce soil temperature and thus improve plant growth. In addition, corn growers typically use high-stature varieties that inevitably incur excessive respiratory carbon loss from roots and transpiration water loss under high night temperature conditions. The main objective of this study was to see if root-zone soil temperature can be reduced through drip irrigation applied at night-time, vs. daytime, using three corn hybrids of different above-ground architecture in Uvalde, TX where day and night temperatures during corn growing season are above U.S. averages. The experiment was conducted in 2014. Our results suggested that delivering well-water at night-time through drip irrigation reduced root-zone soil temperature by 0.6 °C, increase root length five folds, plant height 2%, and marginally increased grain yield by 10%. However, irrigation timing did not significantly affect leaf chlorophyll level and kernel crude protein, phosphorous, fat and starch concentrations. Different from our hypothesis, the shorter, more compact corn hybrid did not exhibit a higher yield and growth as compared with taller hybrids. As adjusting irrigation timing would not incur an extra cost for farmers, the finding reported here had immediate practical implications for farm scale adaptation to hot environments.

Description: Drought at pre-anthesis stages can influence barley growth and results in yield losses. Therefore, it is important to understand how drought at pre-anthesis can affect different traits associated with yield reduction in barley. The objective of this study was to understand the relevance of the genetic background of major flowering time genes in barley plants subjected to pre-anthesis drought and its impact on yield and yield components. A glasshouse experiment using a Randomized Complete Block Design was conducted to investigate the effect of drought and its timing on yield and yield components on eleven barley genotypes, which were selected to represent genetic diversity of major flowering time genes (PPDH1, PPDH2, HvVrn1, HvVrn2 and HvVrn3). Barley plants were exposed to three water regimes, non-stressed and stressed, which was applied at two pre-anthesis growth stages, tillering (SS) and stem elongation (SE). Results identified differences among genotypes in all measured traits. Grain yield, grain number and "thousand kernel weight" were reduced in all genotypes due to drought, irrespective of the growth stage. Early flowering genotypes had better performance as reflected in higher yield compared with late flowering genotypes. Results verified the fundamental importance of early flowering to improve productivity in response to pre-anthesis drought. The results of this study can help in selecting barley lines for future breeding purposes with improved resilience to drought conditions in Mediterranean environments.

Description: Aflatoxins, produced by the fungus Aspergillus flavus, contaminate maize grain and threaten human food and feed safety. Plant resistance is considered the best strategy for reducing aflatoxin accumulation. Six maize germplasm lines, TZAR101–TZAR106, were released by the International Institute of Tropical Agriculture-Southern Regional Research Center (IITA-SRRC) maize breeding collaboration for use in African National Programs and U.S. maize breeding programs. The present investigation was conducted to evaluate aflatoxin reduction by these lines in a U.S. environment. As germplasm lines, resistance was demonstrated by the lines tested in 2010 and 2014 trials. In 2010, TZAR106 was among the lines with the lowest toxin accumulation, and in 2014, along with TZAR102, supported low aflatoxin. When evaluated as single cross hybrids in 2012, 2013 and 2014, several crosses involving IITA-SRRC lines accumulated low toxin. In 2012, TZAR103 × HBA1 was one of 4 lines with the lowest concentration of aflatoxin. In 2014, five IITA-SRRC hybrids were among the lowest with TZAR102 × Va35 and TZAR102 × LH132 being the two lowest. Results demonstrate significant aflatoxin reduction by IITA-SRRC lines in a U.S. aflatoxin-conducive environment (at Mississippi State University). Further testing in different locations and environments is needed to further evaluate the potential usefulness of these germplasm lines.

Description: Nitrogen (N) fertilizers are one of the most expensive inputs in agricultural settings. Additionally, the loss of N increases costs, contributes to soil acidification, and causes off-site pollution of the air, groundwater and waterways. This study reviews current knowledge about technologies for N fertilization with potential to increase N use efficiency and reduce its negative effects on the environment. Classic inorganic sources such as urea and ammonium sulfate are the major sources utilized, while controlled N release fertilizers have not been significantly adopted for cereals and oil crops. Microorganisms, with the exception of Rhizobium sp. in soybeans, are also not widely used nowadays (e.g., plant growth-promoting bacteria and cynobacteria). The interest in implementing new N fertilization knowledge is stimulating the development of sensors to diagnose the N status and decision support systems for integrating several variables to optimize sources, rates and methods of application. Among potential new technologies we identified the incipient development of nanofertilizers, nutrient formulations to coat seeds, and recycled nutrients. Furthermore, increasing concern about the environmental consequences of N may facilitate the implementation of innovations outside the farm such as more effective regulations to guide N fertilization and methods to manufacture N fertilizers that are more energy-efficient and less CO2 equivalent emitting.

Description: Efficient acquisition, assimilation and economy of nitrogen are of special importance in trees that must cope with seasonal periods of growth and dormancy over many years. The ability to accumulate nitrogen reserves and to recycle N determine to a great extent the growth and production of forest biomass. The metabolic relevance of two key amino acids, arginine and phenylalanine, as well as other processes potentially involved in the nitrogen economy of conifers are discussed in the current review. During their long life cycles, conifers not only cope with cyclical annual and long-term changes in the environment but also interact with other organisms such as herbivores and symbionts. The interactions of biotic and abiotic factors with conifer nitrogen metabolism will also be outlined in this review.

Description: The aim of the apricot-breeding program in the Horticultural Faculty in Lednice, that has been developed since 1981, is to obtain new adaptable cultivars, which combine most of the valuable biological traits. Standard breeding techniques, such as crossing by emasculation and hand pollination, self-pollination and open pollination, were employed. A total of 1.154 crossings were produced from more than 110 different parents. So far more than 20,000 seedlings have been obtained, of which about 13,000 have already been evaluated. The most interesting selections were grafted and planted in trial orchards (we now have more than 650 elite genotypes). Ten of these have already been registered, and further promising new hybrids have been submitted for registration and law right protection (Betinka, Candela, Sophia and Adriana). The basic prerequisite for the initiation of the breeding program was a large collection of genetic resources, established and gradually supplemented since the 1970s. At the present time, we preserve and manage more than 300 accessions of apricot trees. In the frame of the descriptive work of the genetic resources and in order to use in breeding, a collection of apricots has been evaluated. We selected the genotypes and characters relating to an increased level of adaptation to the environment.

Description: In the Sahel zone of West Africa that extends from Senegal to Chad, temperatures can vary from less than 15 °C to 25 °C from November to February. These low temperatures affect the growth, development and yield of rice plants, and therefore constitute a major constraint to rice production in the Sahel. In order to identify rice varieties tolerant to cold stress at different developmental stages, a diverse set of 224 rice germplasm was evaluated for yield and yield-related traits in Ndiaye, Senegal, using three different sowing dates. The first sowing date (October 2010), was chosen so as to expose the rice plants to cold stress at the reproductive stage while the rice crop planted at the second sowing date (January 2011) experienced cold stress at the vegetative stage. The third sowing date (July 2011) was the normal planting date for irrigated rice in the Sahel and it served as the control date when the crop does not experience any cold stress throughout its growth cycle. Among the data collected, significant genetic variation was detected and genotype-by-environment interaction was also significant for the traits. At the vegetative stage, cold stress reduced tillering and plant vigor and delayed flowering but increased yield, whereas at the reproductive stage, aside from delaying flowering, cold stress also inhibited panicle exsertion and reduced panicle length, spikelet fertility, grain filling and strongly reduced yields. Principal Component Analysis and correlation analysis using agro-morphological traits helped to identify genotypes that were tolerant to cold stress at either the vegetative or the reproductive stage and the traits associated with high yield under cold stress at each of these stages. Our results can be used to develop cold tolerant rice varieties adapted to double cropping in the Sahelian zone of West Africa.

Description: The aim of this study was to investigate the role of ASN3-encoded asparagine synthetase (AS, EC 6.3.5.4) during vegetative growth, seed development and germination of Arabidopsis thaliana. Phenotypic analysis of knockout (asn3-1) and knockdown (asn3-2) T-DNA insertion mutants for the ASN3 gene (At5g10240) demonstrated wild-type contents of asparagine synthetase protein, chlorophyll and ammonium in green leaves at 35 days after sowing. In situ hybridization localized ASN3 mRNA to phloem companion cells of vasculature. Young siliques of the asn3-1 knockout line showed a decrease in asparagine but an increase in glutamate. The seeds of asn3-1 and asn3-2 displayed a wild-type nitrogen status expressed as total nitrogen content, indicating that the repression of ASN3 expression had only a limited effect on mature seeds. An analysis of amino acid labeling of seeds imbibed with (15N) ammonium for 24 h revealed that asn3-1 seeds contained 20% less total asparagine while 15N-labeled asparagine ((2-15N)asparagine, (4-15N)asparagine and (2,4-15N)asparagine) increased by 12% compared to wild-type seeds. The data indicate a fine regulation of asparagine synthesis and hydrolysis in Arabidopsis seeds.

Description: The bioenergy industry produces a wide range of byproducts varying in their chemical composition depending on type of technology employed. In particular, pyrolysis and transesterification conversion processes generate C-rich byproducts of biochar (BC) and glycerol (GL), respectively, which can be added to soil. These two byproducts vary in their carbon availability, and comparing their effects when added to agricultural soil deserves attention. This study investigated the immediate and residual effects of a single application of BC and GL to a cultivated Brown Chernozem soil from the semi-arid region of southwestern Saskatchewan, Canada. In the first season following addition of amendments, BC and GL alone had no significant impact on all measured parameters. However, when combined with 50 kg urea N·ha−1 (BC + UR), the yields obtained were similar to those with 100 kg urea N·ha−1 alone. The GL with urea N (GL + UR) treatment had reduced crop yield and N uptake compared to urea alone in the year of application attributed to N immobilization, but had a positive residual effect in the second year due to remineralization. Both GL and GL + UR treatments enhanced dehydrogenase activity compared to other treatments whereas BC + UR tended to decrease microbial biomass C. The crop and soil response to application of biochar was less than observed in previous studies conducted elsewhere. Direct and residual effects of glycerol addition on the crop were more evident. An application rate greater than 2.8 t·ha−1 and 3.5 t·ha−1 for BC and GL, respectively, may be required to induce larger responses.

Description: Lentil plays a major role in the food and nutritional security of low income Ethiopian families because of the high protein content of their seed; however, their productivity typically is low largely due to soil fertility limitations. Field and pot experiments were conducted during the 2011 cropping season to determine the effectiveness of Rhizobium strains on two cultivars of lentil in Southern Ethiopia. Six rhizobial inoculant treatments (four indigenous and two commercial inoculants), a nitrogen (N) fertilizer treatment (50 kg·urea·ha−1) and an absolute control (non-inoculated non-fertilized) were used. Inoculated plants produced significantly higher nodule number, nodule dry weight, grain yield and yield components than non-inoculated non-fertilized plants. Inoculation of field grown lentil with rhizobia strain Lt29 and Lt5 enhanced seed yield by 59% and 44%, respectively. Whereas urea fertilization enhanced yields by 40%. Similarly, grain yields were increased during the pot experiment by 92% and 67% over the control treatments by inoculation with Lt29 and Lt5, respectively. The highest levels of N fixation were achieved in plants inoculated with Lt29 (65.7% Ndfa). Both field and pot investigations indicate that inoculation of lentil with native rhizobial strains replace the need for inorganic N fertilization to optimize lentil yields.

Description: Extremes in soil mineral supply alter the metallome of seeds much less than that of their herbage. The underlying mechanisms of mineral homeostasis and the “puzzle of seed filling” are not yet understood. Field crops of wheat, rye, pea, and the mushroom Kuehneromyces mutabilis were established on a set of metalliferous uranium mine soils and alluvial sands. Mineral concentrations in mature plants were determined from roots to seeds (and to fungal basidiospores) by ICP-MS following microwave digestion. The results referred to the concentrations of soil minerals to illustrate regulatory breaks in their flow across the plant sections. Root mineral concentrations fell to a mean of 7.8% in the lower stem of wheat in proportions deviating from those in seeds. Following down- and up-regulations in the flow, the rachis/seed interface configured with cuts in the range of 1.6%–12% (AsPbUZn) and up-regulations in the range of 106%–728% (CuMgMnP) the final grain metallome. Those of pea seeds and basidiospores were controlled accordingly. Soil concentration spans of 9–109× in CuFeMnNiZn shrank thereby to 1.3–2× in seeds to reveal the plateau of the cultivar’s desired target metallome. This was brought about by adaptations of the seed:soil transfer factors which increased proportionally in lower-concentrated soils. The plants thereby distinguished chemically similar elements (As/P; Cd/Zn) and incorporated even non-essential ones actively. It is presumed that high- and low-concentrated soils may impair the mineral concentrations of phloems as the donors of seed minerals. In an analytical and strategic top performance, essential and non-essential phloem constituents are identified and individually transferred to the propagules in precisely delimited quantities.

Description: Efforts to improve water-soluble carbohydrate (WSC) concentrations are common in perennial forage grass breeding. Perennial ryegrass (Lolium perenne) breeding has been very successful in developing new cultivars with high WSC and high agronomic performance. Breeding efforts are ongoing to improve the WSC of other perennial forage grasses, such as orchardgrass (Dactylis glomerata). The United States Department of Agriculture Forage and Range and Deutsche Saatveredelung orchardgrass breeding programs cooperated to characterize the expression and genotype by environment interaction (GEI) of water-soluble carbohydrates in a collection of orchardgrass populations from both breeding programs. Additionally, the effort characterized the relationship between water-soluble carbohydrates and other agronomic and nutritive value traits in these populations. Overall, the Deutsche Saatveredelung populations had higher herbage mass (15%), rust resistance (59%), and later maturity. The Forage and Range Research populations had higher water-soluble carbohydrates (4%), nutritive value, and earlier maturity. However, results were highly dependent on GEI. Differences were very pronounced at the French and German field locations, but less pronounced at the two US locations. Combining the germplasm from the Forage and Range Research and Deutsche Saatveredelung programs may be a way to develop an improved base germplasm source that could then be used separately in the EU and US for water-soluble carbohydrate and other trait improvement.

Description: For many decades, it was commonly believed that humic acids were formed in soils by the microbial conversion of plant lignins. However, an experiment to test whether these humic acids were formed prior to plant matter reaching the soil was never reported until the late 1980s (and then only as a side issue), even though humic acids were first isolated and reported in 1786. This was a serious omission, and led to a poor understanding of how the humic acid content of soils could be maintained or increased for optimum fertility. In this study, commercial sugar cane mulch and kelp extracts were extracted with alkali and analyzed for humic acid content. Humic acids in the extracts were positively identified by fluorescence spectrophotometry, and this demonstrated that humic acids are formed in senescent plant and algal matter before they reach the soil, where they are then strongly bound to the soil and are also resistant to microbial metabolism. Humic acids are removed from soils by wind and water erosion, and by water leaching, which means that they must be regularly replenished. This study shows that soils can be replenished or fortified with humic acids simply by recycling plant and algal matter, or by adding outside sources of decomposed plant or algal matter such as composts, mulch, peat, and lignite coals.

Description: Unique, variable summer climate of the lower Mississippi (MS) Delta region poses a critical challenge to cotton producers in deciding when to plant for optimized production. Traditional 2–4 year agronomic field trials conducted in this area fail to capture the effects of long-term climate variabilities in the location for developing reliable planting windows for producers. Our objective was to integrate a four-year planting-date field experiment conducted at Stoneville, MS during 2005–2008 with long-term climate data in an agricultural system model and develop optimum planting windows for cotton under both irrigated and rainfed conditions. Weather data collected at this location from 1960 to 2015 and the CSM-CROPGRO-Cotton v4.6 model within the Root Zone Water Quality Model (RZWQM2) were used. The cotton model was able to simulate both the variable planting date and variable water regimes reasonably well: relative errors of seed cotton yield, aboveground biomass, and leaf area index (LAI) were 14%, 12%, and 21% under rainfed conditions and 8%, 16%, and 15% under irrigated conditions, respectively. Planting windows under both rainfed and irrigated conditions extended from mid-March to mid-June: windows from mid-March to the last week of May under rainfed conditions, and from the last week of April to the end of May under irrigated conditions were better suited for optimum yield returns. Within these windows, rainfed cotton tends to lose yield from later plantings, but irrigated cotton benefits; however, irrigation requirements increase as the planting windows advance in time. Irrigated cotton produced about 1000 kg·ha−1 seed cotton more than rainfed cotton, with irrigation water requirements averaging 15 cm per season. Under rainfed conditions, there is a 5%, 14%, and 27% chance that the seed cotton production is below 1000, 1500, and 2000 kg·ha−1, respectively. Information developed in this paper can help MS farmers in decision support for cotton planting.

Description: Post-rice irrigated soils offer several potential advantages for the growth of subsequent crops, but Australian producers have often been reluctant to grow grain legumes immediately following a rice crop due to physico-chemical constraints. A field experiment was thus conducted to explore the potential for producing grain legumes following rice in comparison to those following a fallow during 2012 and 2013. Two grain legumes, field pea and faba bean, were sown 5, 7 and 12 weeks after rice harvest in 2013 at Yanco, NSW, and plant growth indicators and grain yield were compared. Early sowing of field pea following rice gave the best outcome, with plants flowering three weeks earlier and yielding 1330 kg·ha−1 more grain than after fallow. In contrast, faba bean yield was 35 kg·ha−1 less after rice than after fallow across the three sowing dates. Higher pea yield was consistent with the early emergence of seedlings, higher light interception and overall greater plant growth following rice. Post-rice crops also had 10-fold less weed infestation than crops in a similarly-established fallow treatment and, thus, required far less weed management. Legume crops sown at the later seeding date had significantly reduced (~50%–60%) yields compared to those of the first two sowings; this is most likely a reflection of reduced temperatures and day lengths experienced during vegetative and reproductive growth phases.

Description: Soil humic substances (HS) are known to be beneficial for soils and plants, and most published studies of HS and humates, usually conducted under controlled conditions, show benefits. However, the value of commercial humate application in the field is less certain. This review attempts to answer the question: How effective are commercial humates in the field? Commercial humates, especially K humate, are used widely in agriculture today as “soil conditioners”. A wide range of benefits is claimed, including growth of beneficial soil microbes; deactivation of toxic metals; improvements in soil structure including water retention capacity, enhanced nutrient and micronutrient uptake and photosynthesis; resistance to abiotic stress, including salinity; and increased growth, yield and product quality. Despite this, there is a surprising lack of solid evidence for their on-farm effectiveness and findings are often inconsistent. The industry relies largely on anecdotal case studies to promote humates, which are often applied at unrealistically low levels. It is recommended that products should be well characterised, physically and chemically, and that careful field studies be conducted on foliar humate application and pelletised humates at realistic rates, targeted to the seedling rhizosphere, for a variety of crops in a range of soils, including low C sandy and saline soils.

Description: The accumulation of biomass and mineral nutrients during the post-anthesis period was investigated in field-grown rice plants cultivated in phosphorus (P)-sufficient vs. P-deficient soil. Phosphorus deficiency reduced biomass accumulation by around 30%, and reduced the accumulation of all nutrients in aboveground plant biomass except sulfur (S) and copper (Cu). Ultimately, grain zinc (Zn), Cu, and calcium (Ca) concentrations were significantly higher in P-deficient plants, while grain magnesium (Mg) concentrations were significantly lower. While P deficiency caused a 40% reduction in the concentration of the anti-nutrient phytate in the grain, this was offset by a 40% reduction in grain starch lysophospholipids, which have positive benefits for human health and grain quality.

Description: Surface soil sodicity as well as subsoil salinity, acidity, and phytotoxic concentrations of chloride (Cl) are major soil constraints to crop production in many soils of sub-tropical, north-eastern Australia. The identification of genotypes tolerant to these soil constraints may be an option to maintain and improve productivity on these soils. We evaluated performance of 11 barley and 17 wheat genotypes grown on two sites 〈0.5 km apart. Compared to the non-sodic site, the sodic site had significantly higher Cl concentration (>800 mg·Cl·kg−1) in the subsoil (0.9–1.3 m soil depth) and higher exchangeable sodium percentage (ESP) (>6%) in the surface and subsoil. Barley grain yield and plant available water capacity (PAWC) were reduced between 5%–25% and 40%–66%, respectively, for different genotypes at the sodic site as compared to the non-sodic site. For wheat genotypes, grain yield was between 8% and 33% lower at the sodic site compared to the non-sodic site and PAWC was between 3% and 37% lower. Most barley and wheat genotypes grown at the sodic site showed calcium (Ca) deficiency symptoms on younger leaves. Analysis of the youngest fully mature leaf (YML) confirmed that genotypes grown at the sodic site with Ca concentration 〈 0.2% exhibited deficiency symptoms. Grain yields of both barley and wheat genotypes grown on the sodic and non-sodic sites increased significantly with increasing Ca and K in YML and decreased significantly with increasing Na and Cl concentrations in YML. Sodium (Na) concentrations in YML of wheat genotypes grown at the sodic site were 10-fold higher than those from the non-sodic site whereas this increase was only two-fold in barley genotypes. In step-wise regression, the PAWC of barley and wheat genotypes grown on sodic and non-sodic sites was the principal determinant of variability of barley and wheat grain yield. Including the Ca concentration in the YML of wheat genotypes and K:Na ratio in the YML of barley genotypes significantly improved the prediction of grain yield in the regression analysis. Barley genotypes, Mackay and Kaputar, were relatively susceptible while Baronesse and Grout were relatively more tolerant to sodicity. Wheat genotypes Gregory and Stampede were generally relatively more susceptible to sodicity, and genotypes Baxter, Hume, and the experimental line HSF1-255 were relatively more tolerant than the former group.

Description: Molecular breeding tools, such as genetic modification, provide forage plant breeders with the opportunity to incorporate high value traits into breeding programs which, in some cases, would not be available using any other methodology. Despite the potential impact of these traits, little work has been published that seeks to optimize the strategies for transgenic breeding or incorporate transgenic breeding with other modern genomics-assisted breeding strategies. As the number of new genomics assisted breeding tools become available it is also likely that multiple tools may be used within the one breeding program. In this paper we propose a strategy for breeding genetically-modified forages using perennial ryegrass as an example and demonstrate how this strategy may be linked with other technologies, such as genomic selection. Whilst the model used is perennial ryegrass the principles outlined are valid for those designing breeding strategies for other outcrossing forage species.

Description: Use of electromagnetic induction (EMI) sensors along with geospatial modeling provide a better opportunity for understanding spatial distribution of soil properties and crop yields on a landscape level and to map site-specific management zones. The first objective of this research was to evaluate the relationship of crop yields, soil properties and apparent electrical conductivity (ECa) at different topographic positions (shoulder, backslope, and deposition slope). The second objective was to examine whether the correlation of ECa with soil properties and crop yields on a watershed scale can be improved by considering topography in modeling ECa and soil properties compared to a whole field scale with no topographic separation. This study was conducted in two headwater agricultural watersheds in southern Illinois, USA. The experimental design consisted of three basins per watershed and each basin was divided into three topographic positions (shoulder, backslope and deposition) using the Slope Position Classification model in ESRI ArcMap. A combine harvester equipped with a GPS-based recording system was used for yield monitoring and mapping from 2012 to 2015. Soil samples were taken at depths from 0–15 cm and 15–30 cm from 54 locations in the two watersheds in fall 2015 and analyzed for physical and chemical properties. The ECa was measured using EMI device, EM38-MK2, which provides four dipole readings ECa-H-0.5, ECa-H-1, ECa-V-0.5, and ECa-V-1. Soybean and corn yields at depositional position were 38% and 62% lower than the shoulder position in 2014 and 2015, respectively. Soil pH, total carbon (TC), total nitrogen (TN), Mehlich-3 Phosphorus (P), Bray-1 P and ECa at depositional positions were significantly higher compared to shoulder positions. Corn and soybeans yields were weakly to moderately (〈±0.75) correlated with ECa. At the deposition position at the 0–15 cm depth ECa-H-0.5 was weakly correlated (r 〈 ±0.50) with soil pH and was moderately correlated (r = ±0.50–±0.75) with organic matter (OM), calcium (Ca) and sulfur (S). Slope variation from 1%–20% at the research site had a strong influence on soil properties at watershed scale. When data from all topographic positions were combined together in all basins spatial interpolation between Mehlich-3 P and ECa-H-0.5 resulted in a larger cross validation RMSE compared to individual shoulder and backslope positions. Results demonstrated that topographic position should be considered while making correlations of ECa with soil properties. Methods of delineating topography positions presented in this paper can easily be replicated on other fields with similar landscape characteristics and EMI sensor based survey techniques can certainly improve and help in making detailed prediction maps of soil properties.

Description: Fermentation quality of dwarf napiergrass (Pennisetum purpureum Schumach) was estimated for additives lactic acid bacteria and Acremonium cellulase (LAB + AC), fermented juice of epiphytic lactic acid bacteria (FJLB), and a no-additive control in 2006 via two ensiling methods—round-bale and vinyl-bag methods in 2006—and via two ensiling seasons—summer and autumn of 2013. Fermentation quality of dwarf napiergrass ensiled in the summer season was improved by the input of additives, with the highest quality in LAB + AC, followed by FJLB; the lactic acid content was higher, and the pH and sum of the butyric, caproic, and valeric acid contents were lower, resulting in an increase in the V-score value by each additive. The ensiling method in autumn without additives affected fermentation quality, mainly due to the airtightness, which was higher for round-bale processing than in vinyl bags, even with the satisfactory V-score of 72. Fermentation in round bales without additives had a higher quality in autumn than in summer, possibly due to the higher concentration of mono- and oligo-saccharides. Thus, it was concluded that dwarf napiergrass can be produced to satisfactory-quality silage by adding LAB + AC or FJLB in summer and even in the absence of additives in autumn.

Description: In this study, the effects of three different main preparatory tillage operations: ploughing at 0.4 m (P40) and 0.20 m (P20) depth and harrowing at 0.20 m depth (MT) were investigated. The tillage operations were carried out at two different times, as the soil water content increased over time from rainfall: (low, 58% (LH) and high, 80% (HH) of field capacity). Results obtained from the soil monitoring carried out before and after tillage showed high values of soil strength in terms of Penetration resistance and shear strength particularly in deeper soil layers at lower water content. During tillage, fossil-fuel energy requirements for P40 LH and P20 LH were 25% and 35% higher, respectively, with respect to the HH treatments and tractor slip was very high (P40 LH = 32.4%) with respect to the P40 HH treatment (16%). Soil water content significantly influenced tractor performance during soil ploughing at 0.40 m depth but no effect was observed for the MT treatment. The highly significant linear relations between grain yield and soil penetration resistance highlight how soil strength may be good indicator of soil productivity. We conclude that ploughing soil to a 0.20 m depth or harrowing soil to a 0.20 m depth is suitable for this type of soil under climate change scenarios.

Description: Italian ryegrass is one of the most important temperate forage grasses on a global basis. Improvement of both dry matter yield and quality of herbage have been major objectives of pasture grass breeding over the last century. F1 and F2 progeny sets derived from controlled pair-crosses between selected Italian ryegrass genotypes have been evaluated for yield and nutritive quality under field conditions. Linear regression of the performance of F1 families under sward conditions on parental genotype means in a spaced plant trial was significant for quality characteristics, but not for herbage yield. This result suggests that phenotypic selection of individual plants from spaced plant nursery is feasible for improvement of nutritive quality traits, but not for yield. The presence of significant heterosis within F1 populations was demonstrated by reduced herbage production in subsequent F2 populations (generated by one cycle of full-sib mating), an up to 22.1% total herbage yield in fresh weight, and a 30.5% survival rate at the end of the second reproductive cycle. Potential optimal crosses for exploiting such heterosis are discussed, based on construction and the inter-mating of complementary parental pools, for the implementation of a novel F1 hybrid production strategy.

Description: Connected multiparental crosses are valuable for detecting quantitative trait loci (QTL) with multiple alleles. The objective of this study was to show that the progeny of a polycross can be considered as connected mutiparental crosses and used for QTL identification. This is particularly relevant in outbreeding species showing strong inbreeding depression and for which synthetic varieties are created. A total of 191 genotypes from a polycross with six parents were phenotyped for plant height (PH) and plant growth rate (PGR) and genotyped with 82 codominant markers. Markers allowed the identification of the male parent for each sibling and so the 191 genotypes were divided into 15 full-sib families. The number of genotypes per full-sib family varied from 2 to 28. A consensus map of 491 cM was built and QTL were detected with MCQTL-software dedicated to QTL detection in connected mapping populations. Two major QTL for PH and PGR in spring were identified on linkage groups 3 and 4. These explained from 12% to 22% of phenotypic variance. The additive effects reached 12.4 mm for PH and 0.11 mm/C°d for PGR. This study shows that the progenies of polycrosses can be used to detect QTL.

Description: The yield response of various crops to salinity under field conditions is affected by soil processes and environmental conditions. The composition of dissolved ions depend on soil chemical processes such as cation or anion exchange, oxidation-reduction reactions, ion adsorption, chemical speciation, complex formation, mineral weathering, solubility, and precipitation. The nature of cations and anions determine soil pH, which in turn affects crop growth. While the ionic composition of soil solution determine the osmotic and ion specific effects on crops, the exchangeable ions indirectly affect the crop growth by influencing soil strength, water and air movement, waterlogging, and soil crusting. This review mainly focuses on the soil chemistry processes that frustrate crop salinity tolerance which partly explain the poor results under field conditions of salt tolerant genotypes selected in the laboratory.

Description: Rice (Oryza sativa L.) is an important staple crop that feeds more than one half of the world’s population and is the model system for monocotyledonous plants. However, rice is very sensitive to salinity and is the most salt sensitive cereal crop with a threshold of 3 dSm−1 for most cultivated varieties. Despite many attempts using different strategies to improve salinity tolerance in rice, the achievements so far are quite modest. This review aims to discuss challenges that hinder the improvement of salinity stress tolerance in rice as well as potential opportunities for enhancing salinity stress tolerance in this important crop.

Description: Most of the Western World banned the cultivation of Cannabis sativa in the early 20th century because biotypes high in ∆9-tetrahydrocannabinol (THC, the principal intoxicant cannabinoid) are the source of marijuana. Nevertheless, since 1990, dozens of countries have authorized the licensed growth and processing of “industrial hemp” (cultivars with quite low levels of THC). Canada has concentrated on hemp oilseed production, and very recently, Europe changed its emphasis from fiber to oilseed. The USA, historically a major hemp producer, appears on the verge of reintroducing industrial hemp production. This presentation provides updates on various agricultural, scientific, social, and political considerations that impact the commercial hemp industry in the United States and Canada. The most promising scenario for the hemp industry in North America is a continuing focus on oilseed production, as well as cannabidiol (CBD), the principal non-intoxicant cannabinoid considered by many to have substantial medical potential, and currently in great demand as a pharmaceutical. Future success of the industrial hemp industry in North America is heavily dependent on the breeding of more productive oilseed cultivars, the continued development of consumer goods, reasonable but not overly restrictive regulations, and discouragement of overproduction associated with unrealistic enthusiasm. Changing attitudes have generated an unprecedented demand for the cannabis plant and its products, resulting in urgent needs for new legislative, regulatory, and business frameworks, as well as scientific, technological, and agricultural research.

Description: Since silage maize is the main crop grown for biogas production (biomass crop) in Germany; its increasing cultivation is critically discussed in terms of social and agronomical aspects. To investigate if sugar beet is suitable as an alternative biomass crop to silage maize; three-year field trials with both biomass crops in rotations with winter wheat (food crop) and continuous cultivation were conducted at three highly productive sites. Dry matter (DM) yield per hectare was measured via field trials whereas methane yield per hectare was estimated via a calculation. Higher annual DM yield was achieved by silage maize (19.5–27.4 t∙ha−1∙a−1) compared to sugar beet root (10.7–23.0 t∙ha−1∙a−1). Dry matter yield was found to be the main driver for the estimated methane yield. Thus; higher estimated methane yield was produced by silage maize (6458–9388 Nm3∙ha−1) with overlaps to sugar beet root (3729–7964 Nm3∙ha−1). We; therefore; classify sugar beet as a suitable alternative biomass crop to silage maize; especially when cultivated in crop rotations with winter wheat. Additionally; we found that the evaluation of entire crop rotations compared to single crops is a more precise approach since it includes rotational effects.

Description: Expanding grain sorghum [Sorghum bicolor (L.) Moench] production northward from southeastern Colorado is thought to be limited by shorter growing seasons due to lower temperatures and earlier frost dates. This study used a simulation model for predicting crop phenology (PhenologyMMS) to estimate the probability of reaching physiological maturity before the first fall frost for a variety of agronomic practices in northeastern Colorado. Physiological maturity for seven planting dates (1 May to 12 June), four seedbed moisture conditions affecting seedling emergence (from Optimum to Planted in Dust), and three maturity classes (Early, Medium, and Late) were simulated using historical weather data from nine locations for both irrigated and dryland phenological parameters. The probability of reaching maturity before the first frost was slightly higher under dryland conditions, decreased as latitude, longitude, and elevation increased, planting date was delayed, and for later maturity classes. The results provide producers with estimates of the reliability of growing grain sorghum in northeastern Colorado.

Description: Many of the major forage species used in agriculture are outcrossing and rely on the exchange of pollen between individuals for reproduction; this includes the major species used for dairy production in grazing systems: perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.). Cultivars of these species have been co-existing since contrasting cultivars were developed using plant breeding, but the consequences and need for strategies to manage co-existence have been made more prominent with the advent of genetic modification. Recent technological developments have seen the experimental evaluation of genetically modified (GM) white clover and perennial ryegrass, although there is no current commercial growing of GM cultivars of these species. Co-existence frameworks already exist for two major cross-pollinated grain crops (canola and maize) in Europe, and for alfalfa (Medicago sativa L.) in the US, so many of the principles that the industry has developed for co-existence in these crops such as detection techniques, segregation, and agronomic management provide lessons and guidelines for outcrossing forage species, that are discussed in this paper.

Description: The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium perenne L.), an important forage species, have advanced to a sufficiently successful and promising stage to merit an exploration of what their further developments may bring. The exploitation of both in vitro and in vivo haploid and DH methods to (1) purge deleterious alleles from germplasm intended for breeding; (2) develop mapping populations for genetic and genomic studies; (3) simplify haplotype mapping; (4) fix transgenes and mutations for functional gene validation and molecular breeding; and (5) hybrid cultivar development are discussed. Even with the comparatively modest budgets of those active in forage crop improvement, haploid and DH techniques can be developed into powerful tools to achieve the acceleration of the speed of genetic gain needed to meet future agricultural demands.

Description: Water use efficiency (WUE) is a trait of prime interest in cases of drought stress because it provides information on biomass production in limited water conditions. In order to get information on WUE and additional water use related traits, i.e., dry weight (DW), fresh weight (FW), total leaf water (LW) and leaf water content (WC), greenhouse pot experiments were conducted on 156 barley genotypes (Hordeum vulgare L.) for control (70% maximal water capacity of soil) and drought stress conditions (20% of the maximal water capacity of soil). Significant correlations between WUE and the other water use related traits (r ≤ 0.65) were determined in juvenile barley, and genotypes suited for improving drought stress tolerance in early developmental stages were identified. Furthermore, based on the significant effects of genotypes and treatments, as well as their interaction, data were used for genome wide association studies (GWAS) resulting in the identification of 14 marker trait associations (MTAs) corresponding to four quantitative trait loci (QTL). For WUE, four MTAs were detected mostly located on barley chromosome 4H. For four MTAs, functional annotations related to the involvement in response to abiotic stress were found. These markers may be of special interest for breeding purposes in cases when they will be validated and also detected in later growth stages.