ALBERT

Description: The growth effects of mixtures are generally assumed to be a result of canopy structure and crown plasticity. Thus, the distribution of leaf area at tree and stand level helps to explain these mixing effects. Therefore, we investigated the leaf area distribution in 12 stands with a continuum of proportions of European larch (Larix decidua Mill.) and Norway spruce (Picea abies (L.) Karst.). The stands were between 40 and 170 years old and located in the northern part of the Eastern Intermediate Alps in Austria at elevations between 900 and 1300 m asl A total of 200 sample trees were felled and the leaf area distribution within their crowns was evaluated. Fitting beta distributions to the individual empirical leaf area distributions, the parameters of the beta distributions were shown to depend on the leaf area of the individual trees and, for spruce, on the proportion of spruce in the stands. With the equations determined, the leaf area distribution of all trees in the stand, and thus its distribution in the stands, was calculated by species and in 2 m height classes. For the individual trees, we found that the leaf area distribution of larch is more symmetric, and its peak is located higher in the crown than it is the case for spruce. Furthermore, the leaf area distribution of both species becomes more peaked and skewed when the leaf area of the trees increases. The mixture only influences the leaf area distribution of spruce in such a way that the higher the spruce proportion of the stand, the higher the leaf area is located within the crown. At the stand level, a strong relationship was found between the proportion of spruce and the distance between the peaks of the leaf area distributions of larch and spruce.

Description: Currently, ultrasonic measurement is a widely used nondestructive approach to determine wood elastic properties, including the dynamic modulus of elasticity (DMOE). DMOE is determined based on wood density and ultrasonic wave velocity measurement. The use of wood average density to estimate DMOE introduces significant imprecision: Density varies due to intra-tree and intra-ring differences and differing silvicultural treatments. To ensure accurate DMOE assessment, we developed a prototype device to measure ultrasonic wave velocity with the same resolution as that provided by the X-ray densitometer for measuring wood density. A nondestructive method based on X-ray densitometry and the developed prototype was applied to determine radial and intra-ring wood DMOE profiles. This method provides accurate information on wood mechanical properties and their sources of variation. High-order polynomials were used to model intra-ring wood density and DMOE profiles in black spruce and jack pine wood. The transition from earlywood to latewood was defined as the inflection point. High and highly significant correlations were obtained between predicted and measured wood density and DMOE. An examination of the correlations between wood radial growth, density, and DMOE revealed close correlations between density and DMOE in rings, earlywood, and latewood

Description: Liriodendron chinense (Hemsl.), a Tertiary relic tree, is mainly distributed in subtropical China. The causes of the geographical distribution pattern of this species are poorly understood. In this study, we inferred historical dispersal routes and glacial refugia of this species by combining genetic data (chloroplast DNA (cpDNA), nuclear ribosomal DNA (nrDNA), and nuclear DNA (nDNA)) and geospatial data (climate and geology) with the methods of landscape genetics. Additionally, based on sequence variation at multiple loci, we employed GenGIS and Barrier software to analyze L. chinense population genetic structure. Dispersal corridors and historical gene flow between the eastern and western populations were detected, and they were located in mountainous regions. Based on species distribution model (SDMs), the distribution patterns in paleoclimatic periods were consistent with the current pattern, suggesting the presence of multiple refuges in multiple mountainous regions in China. The genetic structure analysis clustered most eastern populations into a clade separated from the western populations. Additionally, a genetic barrier was detected between the eastern and western populations. The dispersal corridors and historical gene flow detected here suggested that the mountains acted as a bridge, facilitating gene flow between the eastern and western populations. Due to Quaternary climatic fluctuations, the habitats and dispersal corridors were frequently inhabited by warm-temperate evergreen forests, which may have fragmented L. chinense habitats and exacerbated the differentiation of eastern and western populations. Ultimately, populations retreated to multiple isolated mountainous refugia, shaping the current geographical distribution pattern. These dispersal corridors and montane refugia suggested that the mountains in subtropical China play a crucial role in the conservation of genetic resources and migration of subspecies or related species in this region.

Description: Vegetation indices derived from remote sensing measurements are commonly used to describe and monitor vegetation. However, the same plant community can have a different NDVI (normalized difference vegetation index) depending on weather conditions, and this complicates classification of plant communities. The present study develops methods of classifying the types of plant communities based on long-term NDVI data (MODIS/Aqua). The number of variables is reduced by introducing two integrated parameters of the NDVI seasonal series, facilitating classification of the meadow, steppe, and forest plant communities in Siberia using linear discriminant analysis. The quality of classification conducted by using the markers characterizing NDVI dynamics during 2003–2017 varies between 94% (forest and steppe) and 68% (meadow and forest). In addition to determining phenological markers, canonical correlations have been calculated between the time series of the proposed markers and the time series of monthly average air temperatures. Based on this, each pixel with a definite plant composition can be characterized by only four values of canonical correlation coefficients over the entire period analyzed. By using canonical correlations between NDVI and weather parameters and employing linear discriminant analysis, one can obtain a highly accurate classification of the study plant communities.

Description: Foliar water uptake (FWU) has been investigated in an increasing number of species from a variety of areas but has remained largely understudied in deciduous, temperate tree species from non-foggy regions. As leaf wetting events frequently occur in temperate regions, FWU might be more important than previously thought and should be investigated. As climate change progresses, the number of drought events is expected to increase, basically resulting in a decreasing number of leaf wetting events, which might make FWU a seemingly less important mechanism. However, the impact of drought on FWU might not be that unidirectional because drought will also cause a more negative tree water potential, which is expected to result in more FWU. It yet remains unclear whether drought results in a general increase or decrease in the amount of water absorbed by leaves. The main objectives of this study are, therefore: (i) to assess FWU-capacity in nine widely distributed key tree species from temperate regions, and (ii) to investigate the effect of drought on FWU in these species. Based on measurements of leaf and soil water potential and FWU-capacity, the effect of drought on FWU in temperate tree species was assessed. Eight out of nine temperate tree species were able to absorb water via their leaves. The amount of water absorbed by leaves and the response of this plant trait to drought were species-dependent, with a general increase in the amount of water absorbed as leaf water potential decreased. This relationship was less pronounced when using soil water potential as an independent variable. We were able to classify species according to their response in FWU to drought at the leaf level, but this classification changed when using drought at the soil level, and was driven by iso- and anisohydric behavior. FWU hence occurred in several key tree species from temperate regions, be it with some variability, which potentially allows these species to partly reduce the effects of drought stress. We recommend including this mechanism in future research regarding plant–water relations and to investigate the impact of different pathways used for FWU.

Description: Research Highlights: Warming alters the chemical composition of Cunninghamia lanceolata (Lamb.) Hook, resulting in increased production of macromolecular compounds that protect against heat stress. Background and Objectives: Low latitude forests are experiencing obvious climatic warming; however, the plant physiological responses to warming are not well understood. As warming induces moisture stress, we hypothesized that warming activates metabolites (i.e., lipids, phenolic compounds, amino acids) and causes damage to the leaves, exemplified by the increased concentrations of reactive oxygen species. Materials and Methods: We conducted a warming experiment in a C. lanceolata plantation. Plant physiological traits associated with nutrient status, reactive oxygen species, antioxidant enzymes species, and metabolites were measured. Results: Warming altered the chemical composition of C. lanceolata as it increased C:N ratios of leaves and roots. In particular, the concentrations of N and P in leaves and roots were significantly decreased under the warming condition, which might be related to the biomass production, namely, a dilution effect. Under the warming condition, most of the phospholipid compounds and proteins significantly increased. Leaf C, carbohydrates, amino acids, organic acids, flavonoids, and phenolic compounds were identified to have significantly lower concentrations under the warming treatment than those under the control treatment. These results suggested that moisture stress under the warming treatment may drive C deficiency and metabolic restriction in plants. Conclusions: Under the warming condition, C. lanceolata changed its energy utilization strategy and invested more resources to produce macromolecular compounds for protecting against heat stress. Warming in sub-tropical forests alters plant chemical properties, and thus may have an important consequence for nutrient cycling and soil C sequestration.

Description: The current level of tropospheric ozone (O3) is expected to reduce the net primary production of forest trees. Here, we evaluated the negative effects of O3 on the photosynthetic CO2 uptake of Japanese forest trees species based on their cumulative stomatal O3 uptake, defined as the phytotoxic O3 dose (POD). Seedlings of four representative Japanese deciduous broad-leaved forest tree species (Fagus crenata, Quercus serrata, Quercus mongolica var. crispula and Betula platyphylla var. japonica) were exposed to different O3 concentrations in open-top chambers for two growing seasons. The photosynthesis–light response curves (A-light curves) and stomatal conductance were measured to estimate the leaf-level cumulative photosynthetic CO2 uptake (ΣPn_est) and POD, respectively. The whole-plant-level ΣPn_est were highly correlated with the whole-plant dry mass increments over the two growing seasons. Because whole-plant growth is largely determined by the amount of leaf area per plant and net photosynthetic rate per leaf area, this result suggests that leaf-level ΣPn_est, which was estimated from the monthly A-light curves and hourly PPFD, could reflect the cumulative photosynthetic CO2 uptake of the seedlings per unit leaf area. Although the O3-induced reductions in the leaf-level ΣPn_est were well explained by POD in all four tree species, species-specific responses of leaf-level ΣPn_est to POD were observed. In addition, the flux threshold appropriate for the linear regression of the responses of relative leaf-level ΣPn_est to POD was also species-specific. Therefore, species-specific responses of cumulative photosynthetic CO2 uptake to POD could be used to accurately evaluate O3 impact on the net primary production of deciduous broad-leaved trees.

Description: Because of its high phosphorus (P) demands, it is likely that the abundance, distribution, and N-fixing capacity of Alnus in boreal forests are tightly coupled with P availability and the mobilization and uptake of soil P via ectomycorrhizal fungi (EMF). We examined whether Alnus shifts EMF communities in coordination with increasingly more complex organic P forms across a 200-year-old successional sequence along the Tanana River in interior Alaska. Root-tip activities of acid phosphatase, phosphodiesterase, and phytase of A. tenuifolia-associated EMF were positively intercorrelated but did not change in a predictable manner across the shrub, to hardwood to coniferous forest successional sequence. Approximately half of all Alnus roots were colonized by Alnicola and Tomentella taxa, and ordination analysis indicated that the EMF community on Alnus is a relatively distinct, host-specific group. Despite differences in the activities of the two Alnus dominants to mobilize acid phosphatase and phosphodiesterase, the root-tip activities of P-mobilizing enzymes of the Alnus-EMF community were not dramatically different from other co-occurring boreal plant hosts. This suggests that if Alnus has a greater influence on P cycling than other plant functional types, additional factors influencing P mobilization and uptake at the root and/or whole-plant level must be involved.

Description: Most populations of Scots pine in Spain are locally adapted to drought, with only a few populations at the southernmost part of the distribution range showing maladaptations to the current climate. Increasing tree heights are predicted for most of the studied populations by the year 2070, under the RCP 8.5 scenario. These results are probably linked to the capacity of this species to acclimatize to new climates. The impact of climate change on tree growth depends on many processes, including the capacity of individuals to respond to changes in the environment. Pines are often locally adapted to their environments, leading to differences among populations. Generally, populations at the margins of the species’ ranges show lower performances in fitness-related traits than core populations. Therefore, under expected changes in climate, populations at the southern part of the species’ ranges could be at a higher risk of maladaptation. Here, we hypothesize that southern Scots pine populations are locally adapted to current climate, and that expected changes in climate may lead to a decrease in tree performance. We used Scots pine tree height growth data from 15-year-old individuals, measured in six common gardens in Spain, where plants from 16 Spanish provenances had been planted. We analyzed tree height growth, accounting for the climate of the planting sites, and the climate of the original population to assess local adaptation, using linear mixed-effect models. We found that: (1) drought drove differences among populations in tree height growth; (2) most populations were locally adapted to drought; (3) tree height was predicted to increase for most of the studied populations by the year 2070 (a concentration of RCP 8.5). Most populations of Scots pine in Spain were locally adapted to drought. This result suggests that marginal populations, despite inhabiting limiting environments, can be adapted to the local current conditions. In addition, the local adaptation and acclimation capacity of populations can help margin populations to keep pace with climate change. Our results highlight the importance of analyzing, case-by-case, populations’ capacities to cope with climate change.

Description: Erwinia billingiae S31R1 and Bacillus simplex S11R41, isolated from the rhizosphere of a healthy tree located in a Pinus radiata D. Don plantation with high presence of fungal pathogens, are antagonists of pine root rot fungi Heterobasidion annosum and Armillaria mellea in vitro and in young trees. For effective biocontrol of these pathogens, the bacteria must stably colonize P. radiata roots following their application. To determine root colonization patterns, the bacteria were transformed with stable plasmids encoding green fluorescent protein (GFP). Transformed E. billingiae was visualized on roots 24 days after soil inoculation by confocal and epifluorescence microscopy, and GFP was detected by ELISA 31 days after inoculation. The presence of E. billingiae microcolonies, in some cases in root intercellular spaces, suggests that bacterial growth was active and localized. Fluorescence of B. simplex S11R41 was visualized on P. radiata roots 31 days after inoculation and its colonization pattern changed from scattered cells to localized microcolonies. Although the populations decreased over time, microcolony formation and localization in specific regions of roots indicated that E. billingiae, normally considered to be an epiphyte, and B. simplex can stably colonize roots of P. radiata.

Description: The dryland vegetation and particularly the Acacia-Commiphora woodlands support the livelihoods of approximately 52 million rural households in the Horn of Africa. Aromatic resins are valuable non-wood forest products (NWFPs) derived from Boswellia and Commiphora species in the drylands of this region. The study seeks to answer the following main questions: “What are the ecological and livelihood roles of resin producing species, and the role that people have in either degrading or restoring these ecosystems?” “Who are the participants in frankincense and myrrh production, processing, and trade, and how do these people interact?” “What is the current and potential future economic impact of frankincense and myrrh production and trade at the household level?” “What are the barriers to enhanced economic outcomes?” The study involves the use of PRISMA method—a systematic methodology to identify, select and analyze the recent literature on aromatic resins in relation to such factors as socio-economic situation, livelihood security, value chain, climate change adaptation, ecology and sustainable development in the Horn of Africa. Systematic identification of publications was conducted using several sources, including but not limited to electronic databases for literature search. Web of Science, Social Science Citation Index and Google Scholar and various scientific journals were investigated using search terms and restrictions. A total of 991 references were retrieved, but literature only published between 2003 to 2017 was selected, which led to the use of 51 works for full-text assessment. The results indicate that of the 51 selected studies, 45% focused on ecology and sustainable management, 31% on economic contribution and livelihood security, 20% on production and value chain development, and 4% on climate change adaptation and mitigation. It could be concluded that farmers’ adoption of Boswellia and Commiphora species as economic tree crops in the Horn of Africa has a distinct role in biodiversity conservation and climate change adaptation by contributing to the sustainability of ecosystem functioning as well as improving household incomes and the rural livelihood security in general, and thereby facilitating poverty alleviation.

Description: ATP-binding cassette (ABC) transporters comprise a transport system superfamily which is ubiquitous in eukaryotic and prokaryotic cells. In plants, ABC transporters play important roles in hormone transport and stress tolerance. In this study, 15 BhABC transporters encoded by genes identified from the transcriptome of Betula halophila were categorized into four subfamilies (ABCB, ABCF, ABCG, and ABCI) using structural domain and phylogenetic analyses. Upon B. halophila exposure to exogenous phytohormones and abiotic stressors, gene expression patterns and transcriptional responses for each subfamily of genes were obtained using semi-quantitative RT-PCR analysis. The results demonstrated that expression of most genes belonging to ABCB and ABCG subfamilies changed in response to exogenous phytohormone exposures and abiotic stress. These results suggest that BhABC genes may participate in hormone transport and that their expression may be influenced by ABA-dependent signaling pathways involved in abiotic stress responses to various stressors.

Description: Fusarium circinatum Nirenberg & O’Donnel is listed among the species recommended for regulation as quarantine pests in Europe. Over 60 Pinus species are susceptible to the pathogen and it also causes disease on Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and species in genera such as Picea and Larix. The European Food Safety Authority considers the probability of new introductions—via contaminated seeds, wood material, soil and growing substrates, natural means and human activities—into the EU very likely. Due to early detection, constant surveillance and control measures, F. circinatum outbreaks have officially been eradicated in Italy and France. However, the global spread of F. circinatum suggests that the pathogen will continue to be encountered in new environments in the future. Therefore, continuous surveillance of reproductive material, nurseries and plantations, prompt control measures and realistic contingency plans will be important in Europe and elsewhere to limit disease spread and the “bridgehead effect”, where new introductions of a tree pathogen become increasingly likely as new environments are invaded, must be considered. Therefore, survey programs already implemented to limit the spread in Europe and that could be helpful for other EU countries are summarized in this review. These surveys include not only countries where pitch canker is present, such as Portugal and Spain, but also several other EU countries where F. circinatum is not present. Sampling protocols for seeds, seedlings, twigs, branches, shoots, soil samples, spore traps and insects from different studies are collated and compiled in this review. Likewise, methodology for morphological and molecular identification is herein presented. These include conventional PCR with a target-specific region located in the intergenic spacer region, as well as several real-time PCR protocols, with different levels of specificity and sensitivity. Finally, the global situation and future perspectives are addressed.

Description: Forests fulfill important ecological functions by sustaining nutrient cycles and providing habitats for a multitude of organisms. They further deliver ecosystem services such as carbon storage, protection from erosion, and wood as an important commodity. Trees have to cope in their environment with a multitude of natural and anthropogenic forms of stress. Resilience and resistance mechanisms to biotic and abiotic stresses are of special importance for long-lived tree species. Since trees exist for many decades or even centuries on the same spot, they have to acclimate their growth and reproduction to constantly changing atmospheric and pedospheric conditions. In this special issue, we invited contributions addressing the physiological responses of forest trees to a wide array of different stress factors. Among the eighteen papers published, seventeen covered drought or salt stress as major environmental cues, highlighting the relevance of this topic in times of climate change. Only one paper studied cold stress [1]. The dominance of drought and salt stress studies underpins the need to understand tree responses to these environmental threats from the molecular to the ecophysiological level. The papers contributing to this Special Issue cover these scientific aspects in different areas of the globe and encompass conifers as well as broadleaf tree species. In addition, two studies deal with bamboo (Phyllostachys sp., [1,2]). Bamboo, although botanically belonging to grasses, was included because its ecological functions and applications are similar to those of trees.

Description: Tree growth strongly responds to climate change, especially in semiarid mountainous areas. In recent decades, China has experienced dramatic climate warming; however, after 2000 the warming trend substantially slowed (indicative of a warming hiatus) in the semiarid areas of China. The responses of tree growth in respect to elevation during this warming hiatus are poorly understood. Here, we present the responses of Qinghai spruce (Picea crassifolia Kom.) growth to warming using a stand-total sampling strategy along an elevational gradient spanning seven plots in the Qilian Mountains. The results indicate that tree growth experienced a decreasing trend from 1980 to 2000 at all elevations, and the decreasing trend slowed with increasing elevation (i.e., a downward trend from −10.73 mm2 year−1 of the basal area increment (BAI) at 2800 m to −3.48 mm2 year−1 of BAI at 3300 m), with an overall standard deviation (STD) of 2.48 mm2 year−1. However, this trend reversed to an increasing trend after 2000, and the increasing trends at the low (2550–2900 m, 0.27–5.07 mm2 year−1 of BAI, p 〉 0.23) and middle (3000–3180 m, 2.08–2.46 mm2 year−1 of BAI, p 〉 0.2) elevations were much weaker than at high elevations (3300 m, 23.56 mm2 year−1 of BAI, p 〈 0.01). From 2000–2013, the difference in tree growth with elevation was much greater than in other sub-periods, with an overall STD of 7.69 mm2 year−1. The stronger drought conditions caused by dramatic climate warming dominated the decreased tree growth during 1980–2000, and the water deficit in the 2550–3180 m range was stronger than at 3300 m, which explained the serious negative trend in tree growth at low and middle elevations. After 2000, the warming hiatus was accompanied by increases in precipitation, which formed a wetting–warming climate. Although moisture availability was still a dominant limiting factor of tree growth, the relieved drought pressure might be the main reason for the recent recovery in the tree growth at middle and low elevations. Moreover, the increasing temperature significantly promoted tree growth at 3300 m, with a correlation coefficient between the temperature and BAI of 0.77 (p 〈 0.01). Our results implied that climate change drove different growth patterns at different elevations, which sheds light into forest management under the estimated future climate warming: those trees in low and middle elevations should be paid more attention with respect to maintaining tree growth, while high elevations could be a more suitable habitat for this species.

Description: In order to explore the emission characteristics of volatile organic compounds (VOCs) and different VOC components in airtight environments, polyvinyl chloride laminated plywood (PVC-P), melamine-impregnated paper laminated plywood (MI-P), water-based paint laminated plywood (WP-P) and unfinished plywood (UF-P) were tested as materials in 15 L small environment cabins. VOCs were collected after being sealed for 2 h, 4 h, 6 h, 8 h, 12 h, 18 h, 24 h and 30 h under different loading ratios (1 m2/m3, 1.5 m2/m3, 2 m2/m3, 2.5 m2/m3) and analyzed using a gas chromatography–mass spectrometer. The results show that VOCs gradually increased and tended to be saturated with the increase of time in an airtight environment. The saturation of veneered plywood was faster than unfinished plywood and the pollution degree of three kinds of veneered plywood from lowest to highest was in order of PVC-P, MI-P, WP-P. Aromatic hydrocarbons account for more than half of VOC concentration, and was most obviously affected by the loading ratios. Surfaced laminated plywood can reduce the emission of aromatic hydrocarbons, but also increases the amount of other compounds released. The concentration of VOC-components shows different characteristic curves at different loading ratios due to the influence of decorative materials.

Description: The amount to be invested and the timing of clearcutting are central concerns in timber production. To assess the impact of the initial planting density on optimal economic rotation, we explicitly included the distribution of stand diameter classes and price differences representing the quality of stumpage in a model of forest land expected value (LEV). We selected five initial planting densities of 35-year old China fir (Cunninghamia lanceolata (Lamb.) Hook) plantations to fit the distribution of diameter classes along with stand age using a three-parameter Weibull theoretical growth model and then the Faustmann formula was used to calculate LEVs under different conditions. We found that the difference in the values of the growth rate of the stand volume and the discount rate affected the direction of the impact of initial planting density on the optimal economic rotation. If the value of the growth rate of the stand volume exceeded that of the discount rate, then the initial planting density had a negative impact on optimal economic rotation and vice versa. In addition, the quality effect, which means the shift in diameter class to a higher value attributed to the initial planting density, determined the extent of the impact of the initial planting density on the optimal economic rotation. The proportion of large-sized timber increased at a faster pace in accordance with the age of the stand in stands where the planting density was low compared with the proportion of such timber in stands with a higher initial planting density. The corresponding net stumpage price difference resulted in significant differences in LEVs. We concluded that a low-density stand of China fir was a preferred planting option for obtaining the highest LEV.

Description: In 2003, a new gall-inducing wasp of the genus Ophelimus was detected in the Valparaíso Region (Chile), affecting tree plantations of Eucalyptus globulus Labill and Eucalyptus camaldulensis Dehnh. Since then Ophelimus has been frequently detected in different plantations in Chile, covering a widespread area. A preliminary collaborative study suggests that the micro-wasp detected should be classified as a new Ophelimus species. In this paper, using an integrative approach (including genetic, morphological, and behavioral data), we addressed the delimitation and description of this new species. This study involved the use of brood adult specimens, raised at the laboratory of MIPlagas Ltda., from infested twigs of E. globulus collected in several localities between of Valparaíso and Los Lagos Regions (Chile). Morphological structures were described according to current Eulophidae taxonomic keys, as well as additional traits, such as gall morphology and behavior. Genetic characterization was implemented using a phylogenetic approach, based on a 648 bp specific fragment of the mitochondrial Cytochrome Oxidase I gene (COI 5 region) obtained from collected specimens and available databases (Genbank, NCBI, and BOLDSystem). Specifically, distinctive patterns of variation were detected in traits like gall and antennae morphology, growth habit trends, and a notorious polyphenism in the setae from the sub marginal vein. Overall evidence suggests that this new entity should be considered a new species in Ophelimus, which is henceforth named Ophelimus migdanorum Molina-Mercader.

Description: Traditional timber harvests on steep slopes have been conducted through labor-intensive and sometimes environmentally impactful methods, such as manual felling with chainsaws and extraction using bladed skid trails, winching, or cable yarding. Ground-based mechanized harvesting and primary transportation methods such as cut-to-length harvesters and forwarders have emerged in some parts of the world as low-impact, safe, and efficient alternatives to the aforementioned systems. However, when mechanized operations are used on steep terrain, problems such as poor stability, loss of traction, and increased soil disturbance can occur. Tethered or winch-assisted logging practices are being tested and applied in several countries to adapt to challenges associated with operating equipment on steep slopes while minimizing environmental impact. To better understand the feasibility of these systems, we conducted a designed experiment to quantify changes in soil properties and predicted erosion resulting from varying numbers of passes and payload levels by a forwarder operating on slopes ranging from 27 to 38 degrees. The machine was equipped with two different track configurations, tethered by either a machine-mounted or self-contained winch, in eucalyptus plantations in Brazil. On low slopes, bulk density significantly increased, but it did not increase on steeper slopes; this demonstrates traction winches’ effectiveness at reducing concentrated ground pressures. Rut depths were minimal and decreased with increasing slope classes due to reduced track slippage. Predicted erosion rates were high, primarily due to the extremely steep, long slopes and lack of adequate cover in some portions of the trail, illustrating the importance of proper erosion management practices on steep slopes.

Description: Exponential fertilization is well-known for improving the growth and nutritional status of seedlings during nursery culture. However, less is known about how genetic variability among seedling species influences the effects of fertilization regimes on the growth of seedlings and their nutritional status under varying fertilization levels. Here, we conducted a greenhouse experiment to compare the growth, as well as the nitrogen (N) and phosphorus (P) nutrient concentration responses, of 2- and 3-generation Chinese fir (Cunninghamia lanceolata (Lambert) Hooker) seedlings to the impacts of varying exponential and conventional constant-rate fertilization levels (50, 100, and 200 mg P seedling−1). Seedling size and shoot:root ratios were lower in exponential fertilization than in conventional fertilization. Exponentially fertilized seedlings had higher concentrations of N and P nutrients compared to conventionally fertilized seedlings during low-level fertilization. Conversely, an elevated P concentration was observed in seedlings subjected to conventional fertilization compared to those exposed to exponential fertilization during high-level fertilization. However, there were no significant differences in seedling N and P concentrations between the two fertilization regimes during medium-level fertilization. Furthermore, 2- and 3-generation Chinese fir seedlings differed greatly in their growth and nutritional status. Additionally, there were significant fertilization regime × seedling generation interaction effects on the seedling shoot:root ratio and N concentration. Our findings suggest that exponential fertilization can improve the nutritional status, survival, and growth of Chinese fir seedlings, especially on competitive sites, and that information on both intra-species genetic variability and appropriate fertilizer levels is necessary for the successful fertilization of Chinese fir seedlings, regardless of fertilization regimes.

Description: Hurricanes shape ecosystems. A broad range of forested ecosystems is particularly affected by hurricanes, thus creating the need for studies addressing the effects of these disturbances. There is a long history of hurricane and forest research on the Caribbean island of Puerto Rico. In this study, we present results from a systematic literature review of peer-reviewed articles regarding ecological research conducted in Puerto Rico on the topic of hurricanes and forests published from 1900 through 2017. We present a summary of cyclonic activity on the island during the study period and the results from the systematic literature review within this cyclonic context. We discuss findings in terms of aspects of forests studied, geographical distribution of study areas, and time scales at which research was conducted. These findings allow us to determine what was studied about hurricanes and forests, identify gaps in the information, and suggest possible areas of research and production of new knowledge that recent and future storms can bring. We conclude with recommendations identifying research needs and propose additional approaches to complement existing information. Our goal is to generate future knowledge from hurricane and forest research with the broadest applications possible.

Description: Adelges tsugae Annand (hemlock woolly adelgid, HWA, an invasive insect native to Japan), which causes defoliation and death of Tsuga canadensis (L.) Carrière (eastern hemlock), was introduced to the United States in the early 1950s and has spread throughout much of the range of T. canadensis causing widespread mortality. In 2016 and 2017, we resampled long-term vegetation monitoring plots across five forest types (ecogroups) within Great Smoky Mountains National Park that contained T. canadensis in 2003 (prior to the spread of HWA within the park) to examine changes in the species composition and diversity of the regeneration layer. We hypothesized that compositional changes in the seedling and sapling strata would be driven primarily by the pre-HWA importance value of T. canadensis and relative dominance of Rhododendron maximum L. (rosebay rhododendron), and that species diversity metrics would differ across year depending on ecogroup and the relative dominance of R. maximum. Non-metric multi-dimensional scaling (NMDS) revealed that the seedling and sapling strata of plots with greater pre-HWA importance of T. canadensis and lower basal area of R. maximum generally exhibited greater compositional change between 2003 and 2017. Topo-edaphic variables were also significant in both NMDS ordinations and were associated with the distribution of multiple hardwood species. Species richness, evenness, and Shannon-Wiener diversity varied with strata following the loss of T. canadensis, with the degree and direction of change varying with the dominance of R. maximum.

Description: The Mongolian pine (Pinus sylvestris var. mongolica) is one of the most common tree species in semiarid and arid areas of China, especially in the sand dunes of the Hulunbeier steppe. This study addresses the morphological and physiological characteristics of the Mongolian pine according to sand dune height. Five sites were chosen with various sand dune heights (P1–P5). Nine years after planting, tree growth, leaf area, leaf mass per leaf unit area (LMA), diameter at breast height (DBH), tree height, diameter at root collar (DRC), longest shoot length, carbon isotope composition, and intrinsic water use efficiency (iWUE) were measured to explore the responses of Mongolian pine trees to drought. DBH, tree height, DRC, leaf area, leaf length, and longest shoot length significantly decreased with greater sand dune height (p 〈 0.05). However, the carbon isotope actually increased with dune height (p 〈 0.05). Conversely, the iWUE of current-year pine needles was significantly higher at measurement points P3 (132.29 μmol CO2 mol −1 H2O), P4 (132.96 μmol CO2 mol −1 H2O), and P5 (125.34 μmol CO2 mol −1 H2O) than at the lower points P1 (95.18 ± 9.87 μmol CO2 mol −1 H2O) and P2 (103.10 ± 11.12 μmol CO2 mol −1 H2O). Greater sand dune height increases the distance to groundwater, which in this study led to an increase in iWUE in the Mongolian pines, thus these trees appear to adapt to increased sand dune height by increasing their iWUE and decreasing their leaf area. However, prolonged periods characterized by such adaptations can lead to tree death. We expect these findings to be useful when selecting plantation sites for Mongolian pines in semiarid and arid climates.

Description: The natural abundance of stable nitrogen (N) isotope (δ15N) in plants and soils can reflect N cycling processes in ecosystems. However, we still do not fully understand patterns of plant and soil δ15N at alpine treelines and shrublines in different climate zones. We measured δ15N and N concentration in leaves of trees and shrubs and also in soils along elevational gradients from lower altitudes to the upper limits of treelines and shrublines in subtropical, dry- and wet-temperate regions in China. The patterns of leaf δ15N in trees and shrubs in response to altitude changes were consistent, with lower values occurring at higher altitude in all three climate zones, but such patterns did not exist for leaf Δδ15N and soil δ15N. Average δ15N values of leaves (−1.2‰) and soils (5.6‰) in the subtropical region were significantly higher than those in the two temperate regions (−3.4‰ and 3.2‰, respectively). Significant higher δ15N values in subtro4pical forest compared with temperate forests prove that N cycles are more open in warm regions. The different responses of leaf and soil δ15N to altitude indicate complex mechanisms of soil biogeochemical process and N sources uptake with environmental variations.

Description: The fungus Pseudodidymella fagi is spreading in Europe and causing leaf blotch of European beech, Fagus sylvatica. Between 2008 and 2017, outbreaks of P. fagi were observed on European beech in Switzerland, Germany (also on F. orientalis), Austria, and Slovakia. In Slovenia, leaf blotch symptoms were first observed on F. sylvatica in 2018. P. fagi was identified as the causal agent of the observed symptoms in Slovenia by morphological examinations together with sequencing of the internal transcribed spacer (ITS) region of the rDNA. This study links the fungus to the expansion of the known distribution of the disease to Slovenia, and based on in vitro pathogenicity trials, also to a new potential host, Quercus petraea. The pathogenicity tests confirmed F. sylvatica and F. orientalis as hosts for P. fagi, but not Castanea sativa, where pathogenicity to F. orientalis was proved for first time in vitro. Although Koch’s postulates could not be proven for C. sativa, it seems to be partially susceptible in vitro because some of the inoculation points developed lesions. Additionally, damage to Carpinus betulus related to P. fagi near heavily infected beech trees was observed in vivo but was not tested in laboratory trials. Based on the results and our observations in the field, it is likely that P. fagi has a wider host range than previously thought and that we might be witnessing host switching.

Description: Research Highlights: In this study, the Generally Recognised as Safe (GRAS) compounds were applied in order to study mould-fungi growth on dried Scots pine sapwood. Background and Objectives: The transition to the use of more sustainable wood-material may be possible by applying GRAS compounds that can control and prevent contamination by primary colonising mould fungi. Materials and Methods: Kiln-dried sawn timber was treated with three different GRAS compounds, and different fungal inoculation methods applied in order to investigate differences in the development of fungal communities. Results: Substances based on potassium silicate significantly reduced fungal growth and mould contamination on the studied wood surfaces. By combining wood-surface treatments with GRAS compounds, fungal-area size as predictors and mould grade as response, a partial least squares (PLS) model that makes it possible to predict mould grade on wood surfaces was developed. The PLS model is a key component in the development of a smart grading-systems equipped by e.g. high-speed digital cameras for the early detection of fungal attack on wood surfaces in different applications. However, the measurements based on chemical characterisation should be the next step to take in order significantly to enhance the model and increase the range of robust applications. In the current study, a multivariate model describing the influence of each fungal-covering area on mould grade was presented for the first time.

Description: The challenges to forest health from climate change, globalization, contemporary trade practices and new recreational patterns require effective biosecurity. We asked: How is the biosecurity border for tree health understood and enacted by state and non-state actors? What are the consequences for tree health? Semi-structured interviews (N = 10) were conducted with scientists and other relevant actors (N = 21). The border was understood variously as: a biophysical boundary, often the coast; a geopolitical boundary, usually of the European Union; the points of main inspection focus; dispersed nodes of inspection; a ‘pre-border’ outside of UK; or by the location of detection activities. A wide range of state, non-state and hybrid groups are engaged in border practices. These practices have been altered due to trade and climate changes, are subject to cost and resource priorities and reflect particular knowledge flows and the biological nature of the agents. We suggest that there is an ‘everyone’ as well as ‘everywhere’ border that demands clarification of risks, roles and responsibilities, and we offer practical recommendations. We conclude that tree health border challenges are a manifestation of wider sustainability issues that enable us to explore human–nature relationships, democratic engagement and the pursuit of more sustainable futures.

Description: Currently, forest biomass estimation methods at the regional scale have attracted the greatest attention from researchers, and the development of stand biomass models has become popular a trend. In this study, a total of 5074 measurements on 1053 permanent sample plots were obtained in the Eastern Da Xing’an Mountains, and three additive systems of stand biomass equations were developed. The first additive system (M-1) used stand variables as the predictors (i.e., stand basal area and average height), the second additive system (M-2) utilized stand volume as the sole predictor, and the third additive system (M-3) included both stand volume and biomass expansion and conversion factors (BCEFs) as the predictors. The coefficients of the three model systems were estimated with nonlinear seemingly unrelated regression (NSUR), while the heteroscedasticity of the model residuals was solved with the weight function. The jackknifing technique was used on the residuals, and several statistics were used to assess the prediction performance of each model. We comprehensively evaluated four stand biomass estimation methods (i.e., M-1, M-2, M-3 and a constant BCEF (M-4)). Here, we showed that the (1) three additive systems of stand biomass equations showed good model fitting and prediction performance, (2) M-3 significantly improved the model fitting and performance and provided the most accurate predictions for most stand biomass components, and (3) the ranking of the four stand biomass estimation methods followed the order of M-3 〉 M-2 〉 M-4 〉 M-1. Our results demonstrated these additive stand biomass models could be used to estimate the stand aboveground and belowground biomass for the major forest types in the Eastern Da Xing’an Mountains, although the most appropriate method depends on the available data and forest type.

Description: We present novel evidence of environmental drivers of seedling density in Abies nephrolepis, an alpine and subalpine tree species. Continuous monitoring of natural conditions is required to understand forest ecosystem dynamics. We investigated Abies nephrolepis seedling dynamics in relation to biotic and abiotic factors. The survey, which included the measurement of trees and seedlings, was carried out from March to October in 2016 and 2018. Monitoring sites in the coniferous forests of Seorak Mountain were divided into 27 quadrats. We analyzed relationships using simple and multiple linear regression. The majority of Abies nephrolepis individuals had a diameter at breast height less than l0 cm, and the number of seedlings increased over the study period. This reflects survival and growth due to successive annual mast seeding events. Aspect direction (R2 = 0.201, p 〈 0.05), rock exposure (R2 = 0.364, p 〈 0.001), canopy openness (R2 = 0.322, p 〈 0.05), herbaceous cover (R2 = 0.268, p 〈 0.01), and basal area (R2 = 0.199, p 〈 0.05) show significant linear relationships with seedling density. Seedling density was positively related to rock exposure, canopy openness, and species richness, and there was a negative relationship between herbaceous cover and basal area (p 〈 0.0001). The relative importance of predictor variables was as follows: Rock exposure (40.3%), canopy openness (30.2%), basal area (13.9%), herbaceous cover (11.5%), and species richness (4.1%). Seedling density was most strongly influenced by the presence of large rocks, which provide shelter from harsh winds and a substrate for moss. We conclude that appropriate canopy openness creates a synergistic relationship. We found a positive association between the Abies nephrolepis seedling density in subalpine forests and certain physical environmental factors. Therefore, environmental gradients about the roles of rocks and canopies apply, even in this habitat.

Description: Urban forests are often heavily populated by street trees along right-of-ways (ROW), and monitoring efforts can enhance municipal tree management. Terrestrial photogrammetric techniques have been used to measure tree biometry, but have typically used images from various angles around individual trees or forest plots to capture the entire stem while also utilizing local coordinate systems (i.e., non-georeferenced data). We proposed the mobile collection of georeferenced imagery along 100 m sections of urban roadway to create photogrammetric point cloud datasets suitable for measuring stem diameters and attaining positional x and y coordinates of street trees. In a comparison between stationary and mobile photogrammetry, diameter measurements of urban street trees (N = 88) showed a slightly lower error (RMSE = 8.02%) relative to non-mobile stem measurements (RMSE = 10.37%). Tree Y-coordinates throughout urban sites for mobile photogrammetric data showed a lower standard deviation of 1.70 m relative to 2.38 m for a handheld GPS, which was similar for X-coordinates where photogrammetry and handheld GPS coordinates showed standard deviations of 1.59 m and the handheld GPS 2.36 m, respectively—suggesting higher precision for the mobile photogrammetric models. The mobile photogrammetric system used in this study to create georeferenced models for measuring stem diameters and mapping tree positions can also be potentially expanded for more wide-scale applications related to tree inventory and monitoring of roadside infrastructure.

Description: Increased urbanization and climate change have resulted in the intensification of the urban heat island (UHI) effect, particularly in tropical cities. One of the main causes of UHI is the man-made urban surfaces influencing the radiation budget by absorbing, reflecting, and emitting radiation at various wavelengths. The radiative budget of a city is directly influenced by the urban geometry, surface materials, direct solar radiation and incident angle, and atmospheric diffuse radiation. Vegetation cover, in contrast, can decrease UHI by intercepting radiation and through the process of photosynthesis. Better understanding the effect of urban vegetation on the radiative budget can thus contribute towards the mitigation of the UHI effect and ultimately the development of climate resilient urban spaces. To analyze the contribution of vegetation to the radiative budget of a city, a detailed simulation of the complex interaction between the built environment and the vegetation is required. This study proposes an approach for analyzing the 3-D structure of both vegetation and built environment to quantify the contribution of vegetation to the radiative budget of an urban landscape. In a first step, a detailed 3-D model of Singapore including buildings and vegetation was reconstructed using a combination of free and commercial Earth Observation data. Then, the 3-D Discrete Anisotropic Radiative Transfer (DART) model was repurposed to estimate the radiation absorbed by the urban surfaces accounting for the presence of vegetation cover with changing Leaf Area Density (LAD) conditions. The presence of trees in the scene accounted for a significant reduction of the absorbed radiation by buildings and ground. For example, in the case of a residential low-building neighborhood, although having low tree cover, the reduction of the absorbed radiation by buildings and ground was up to 15.5% for a LAD =1. The field validation shows good agreement (R2 = 0.9633, RMSE = 10.8830 and Bias = −1.3826) between the DART-simulated shortwave exitance and upwelling shortwave measurements obtained from a net radiometer mounted on a local flux tower in the urban area of Singapore, over the studied period. Our approach can be used for neighborhood-scale analysis, at any desired location of a city, to allow test scenarios with varying surface materials and vegetation properties.

Description: In recent decades, the terrestrial ecosystem in Khyber Pakhtunkhwa Province (KPK), Pakistan, has undergone tremendous destruction. To restore environmental conditions, the government implemented the Billion Tree Afforestation Program (BTAP), with a high target to impact multidimensional aspects of the terrestrial ecosystem. The government claims that it is local residents who have benefited the most from the BTAP. Hence, the objective of this research was to examine the socioeconomic impact and local rural perception of the BTAP in three districts of KPK. Primary data were collected from 406 households. Fundamental orientation theory was used to assess the social impacts of the BTAP, while cost–benefit analysis was applied to examine its economic impact. The results show that the overall social impacts of the BTAP are satisfactory and beneficial, increasing social sustainability by 69% between 2014 and 2018. Additionally, based on the cost–benefit analysis and perception-based analysis, it was found that the BTAP positively affects the economic conditions of rural households. The community livelihood increased during the program, with a total net income of 6.9 million USD in the three districts of KPK. It is concluded that the majority of respondents have benefited from participation in the BTAP. The sustainability of rural livelihood is one of the main concerns related to the establishment of the BTAP. Effort is needed by the government and other parties to both increase rural household income and to protect the environment.

Description: The emerald ash borer (Agrilus planipennis Fairmaire; EAB) is an invasive insect that causes mortality of trees in the genus Fraxinus, creating canopy gaps that may facilitate invasion by exotic plants. Planting native tree seedlings under EAB-infested Fraxinus may accelerate succession and preclude invasive plant expansion; however, the effectiveness of this approach has not been experimentally tested. We assessed understory seedling planting of Quercus rubra, Carya laciniosa, and Juglans cinerea in EAB-infested forests, where the invasive shrub Lonicera maackii (Amur honeysuckle) was removed. We tested whether the use of plastic tree shelters (“tree tubes”) or planting season (fall versus spring) contributed to the success of the reforestation plan by measuring growth rates (cm/yr) and survivorship two and seven years after planting. After seven years, seedling survivorship was 〈25% for all species and planting techniques. Quercus rubra exhibited poor survivorship with one seedling surviving to the conclusion of the experiment. Juglans cinerea and C. laciniosa had higher survivability and growth rates than did Q. rubra after two and seven years. Effects of tree tubes were weak and temporary. After 2 years, Q. rubra seedling survivorship was higher in tree tubes; however, by the end of the experiment 29 of the 30 Q. rubra seedlings in tree tubes had died. Juglans cinerea seedlings grew faster when planted in the fall compared to the spring, but overall survivorship of these seedlings was unaffected by planting season. Neither the use of tree shelters nor the planting season contributed to the growth or survival of C. laciniosa seedlings. In summary, our results indicate that seedling planting of Carya and Juglans may be a useful way to increase biodiversity in regenerating forests; however, the resource-expensive processes of over-wintering seedlings and using tree shelters may not increase the success of reforestation efforts.

Description: Forest health can be adversely affected by invasive organisms. Biosecurity measures to prevent the establishment of harmful invasive organisms at national points of entry (e.g., airports or shipping ports) are vital to protect forest health. Innovations in pest eradication technologies are being developed based on their efficiencies and effectiveness. However, the question of whether people find them acceptable is rarely considered. In New Zealand, research is underway into the use of highly targeted pesticide spraying using unmanned aerial vehicles (UAVs) as a novel technology to eradicate pest species that impact forest, food, and fibre sectors. Public approval for such technologies, however, can be a critical aspect for their success. A tool can be technically effective (achieve eradication), but uptake may be impossible if communities do not trust the technology. We developed a method for enabling discussions about the use of UAVs and their acceptability in general before being operationalized for biosecurity. This paper presents an investigation of how “participatory-design”, an often tactile, visual, and inclusive process of community engagement can improve the acceptance of technology use in the public sphere. We asked people, both scientists and citizens, to evaluate the acceptability of a range of UAV uses (including biosecurity) along a continuum and then explored the reasons for their placement. Key criteria for acceptable and unacceptable uses were subsequently developed to help technology designers and operators consider aspects of social acceptability during design processes. Our tool and approach facilitated discussions around technology acceptability that were subsequently adopted by our technical design team for the development and the use of acceptable UAVs for biosecurity. This research shows how systematic approaches to design can help uncover and mitigate social acceptability issues through inclusive design under increasing threats of biosecurity, whether related to challenges of trade or climate change.

Description: We contribute to a better understanding of different remote sensing techniques for tree height estimation by comparing several techniques to both direct and indirect field measurements. From these comparisons, factors influencing the accuracy of reliable tree height measurements were identified. Different remote sensing methods were applied on the same test site, varying the factors sensor type, platform, and flight parameters. We implemented light detection and ranging (LiDAR) and photogrammetric aerial images received from unmanned aerial vehicles (UAV), gyrocopter, and aircraft. Field measurements were carried out indirectly using a Vertex clinometer and directly after felling using a tape measure on tree trunks. Indirect measurements resulted in an RMSE of 1.02 m and tend to underestimate tree height with a systematic error of −0.66 m. For the derivation of tree height, the results varied from an RMSE of 0.36 m for UAV-LiDAR data to 2.89 m for photogrammetric data acquired by an aircraft. Measurements derived from LiDAR data resulted in higher tree heights, while measurements from photogrammetric data tended to be lower than field measurements. When absolute orientation was appropriate, measurements from UAV-Camera were as reliable as those from UAV-LiDAR. With low flight altitudes, small camera lens angles, and an accurate orientation, higher accuracies for the estimation of individual tree heights could be achieved. The study showed that remote sensing measurements of tree height can be more accurate than traditional triangulation techniques if the aforementioned conditions are fulfilled.

Description: Selective logging is one of the main natural forest harvesting approaches worldwide and contributes nearly 15% of global timber needs. However, there are increasing concerns that ongoing selective logging practices have led to decreased forest product supply, increased forest degradation, and contributed to forest based carbon emissions. Taking cases of natural forest harvesting practices from the Tarai region of Nepal and Queensland Australia, this study assesses forest product recovery and associated carbon emissions along the timber production chain. Field measurements and product flow analysis of 127 commercially harvested trees up to the exit gate of sawmills and interaction with sawmill owners and forest managers reveal that: (1) Queensland selective logging has less volume recovery (52.8%) compared to Nepal (94.5%) leaving significant utilizable volume in the forest, (2) Stump volume represents 5.5% of total timber volume in Nepal and 3.9% in Queensland with an average stump height of 43.3 cm and 40.1 cm in Nepal and Queensland respectively, (3) Average sawn timber output from the harvested logs is 36.3% in Queensland against 61% in Nepal, (4) Nepal and Queensland leave 0.186 Mg C m−3 and 0.718 Mg C m−3 on the forest floor respectively, (5) Each harvested tree damages an average of five plant species in Nepal and four in Queensland predominantly seedlings in both sites, and (6) Overall logging related total emissions in Queensland are more than double (1.099 Mg C m−3) those in Nepal (0.488 Mg C m−3). We compared these results with past studies and speculated on possible reasons for and potential implications of these results for sustainable forest management and reducing emissions from deforestation and forest degradation.

Description: The Returning Farmland to Forest Program (RFFP) was implemented in China in 1999 with the goal of supporting environmental restoration by returning significant areas of cultivated land to forest. While afforestation supports long-term ecosystem services like carbon sequestration and the reduction of soil and water loss, it also reduces the amount of available arable land, putting financial pressure on those who depend on it for their livelihoods. In an effort to balance both ecological and economic benefits, regional governments offer financial compensation to farmers to offset these pressures in the form of a dollar amount per hectare of reforested land. The current study explores participants’ willingness to accept pay (WTA), along with the difference between the offered per hectare compensation and the amount deemed acceptable by RFFP participants in the region. To this end, 92 households from the representative afforestation area were surveyed in Huining County, Gansu Province, China - an area of strategic ecological importance in the Loess Plateau. The results showed 12.0% of the surveyed respondents to be satisfied with the current compensation policy, while 88.0% of respondents were not. The respondents’ lower and upper WTA limits were $221/ha/year and $1331/ha/year, respectively, with an average WTA of $777/ha/year. The compensation that respondents would be most willing to accept was distributed in the $444–888/ha/year and the $889–1331/ha/year ranges, accounting for 37.0% and 31.5% of the total responses, respectively. Gender, age, and education were found to be the main factors influencing a respondents’ WTA. Results of the survey suggest that the actual compensation amount ($355/ha/year) is much lower than respondents’ WTA, and that compensation measures and policies should be improved to guarantee a basic income.

Description: To promote Bio-Energy with Carbon dioxide Capture and Storage (BECCS), which aims to replace fossil fuels with bio energy and store carbon underground, and Reducing Emissions from Deforestation and forest Degradation (REDD+), which aims to reduce the carbon emissions produced by forest degradation, it is important to build forest management plans based on the scientific prediction of forest dynamics. For Measurement, Reporting and Verification (MRV) at an individual tree level, it is expected that techniques will be developed to support forest management via the effective monitoring of changes to individual trees. In this study, an end-to-end process was developed: (1) detecting individual trees from Unmanned Aerial Vehicle (UAV) derived digital images; (2) estimating the stand structure from crown images; (3) visualizing future carbon dynamics using a forest ecosystem process model. This process could detect 93.4% of individual trees, successfully classified two species using Convolutional Neural Network (CNN) with 83.6% accuracy and evaluated future ecosystem carbon dynamics and the source-sink balance using individual based model FORMIND. Further ideas for improving the sub-process of the end to end process were discussed. This process is expected to contribute to activities concerned with carbon management such as designing smart utilization for biomass resources and projecting scenarios for the sustainable use of ecosystem services.

Description: Whitebark pine populations are declining nearly range-wide, primarily from the exotic fungal pathogen that causes white pine blister rust (WPBR). Climate change is expected to exacerbate these declines by decreasing climatically suitable areas. Planting WPBR-resistant seedlings is a key restoration action, but it is costly, time consuming, and labor intensive. Direct seeding—sowing seeds rather than planting seedlings—may reduce costs and open remote areas to restoration; however, its efficacy remains largely unexplored. In this case study, we estimated the annual survival rates (ASR) of seedlings grown from directly sown seeds, and the effect of elevation zone and microsite type on survival. For five years we monitored 184 caches containing one or more seedlings within one study area in the Greater Yellowstone Ecosystem. Seed caches were originally stratified between subalpine forest and treeline and among three microsite types defined by a nurse object: Rocks, trees, and no object. To estimate ASR, we selected the most parsimonious model of a set using AICc. ASR was best described by elevation zone and year and ranged from 0.571 to 0.992. The odds of seedling survival were 2.62 times higher at treeline than in subalpine forest and were 4.6 to 36.2 times higher in 2016–2018 than 2014. We estimated the probability that a whitebark pine seed cache would contain one or more living seedlings six years after sowing to be 0.175 and 0.0584 for treeline and subalpine caches, respectively. We estimated that 1410 and 4229 caches ha−1 would need to be sown at treeline and in subalpine forest, respectively, to attain target restoration densities of 247 established trees ha−1. Our findings, although based on one study area, suggest that climate change may be increasing treeline regeneration, and that direct seeding may be a viable restoration option and climate change mitigation tool for whitebark pine.

Description: Knowledge on population diversity and structure is of fundamental importance for conifer breeding programs. In this study, we concentrated on the development and application of high-density single nucleotide polymorphism (SNP) markers through a high-throughput sequencing technique termed as specific-locus amplified fragment sequencing (SLAF-seq) for the economically important conifer tree species, Chinese fir (Cunninghamia lanceolata). Based on the SLAF-seq, we successfully established a high-density SNP panel consisting of 108,753 genomic SNPs from Chinese fir. This SNP panel facilitated us in gaining insight into the genetic base of the Chinese fir advance breeding population with 221 genotypes for its genetic variation, relationship and diversity, and population structure status. Overall, the present population appears to have considerable genetic variability. Most (94.15%) of the variability was attributed to the genetic differentiation of genotypes, very limited (5.85%) variation occurred on the population (sub-origin set) level. Correspondingly, low FST (0.0285–0.0990) values were seen for the sub-origin sets. When viewing the genetic structure of the population regardless of its sub-origin set feature, the present SNP data opened a new population picture where the advanced Chinese fir breeding population could be divided into four genetic sets, as evidenced by phylogenetic tree and population structure analysis results, albeit some difference in membership of the corresponding set (cluster vs. group). It also suggested that all the genetic sets were admixed clades revealing a complex relationship of the genotypes of this population. With a step wise pruning procedure, we captured a core collection (core 0.650) harboring 143 genotypes that maintains all the allele, diversity, and specific genetic structure of the whole population. This generalist core is valuable for the Chinese fir advanced breeding program and further genetic/genomic studies.

Description: In the context of climate change, drought is likely to become more frequent and more severe in urban areas. Urban trees are considered to play an important role in fixing carbon, improving air quality, reducing noise and providing other ecosystem services. However, data on the response of urban trees to climate change, particularly to drought, as well as the relationship between their below- and above-ground processes in this context, are still limited, which prevents a comprehensive understanding of the role of urban trees in ameliorating some of the adverse effects of climate change and their ability to cope with it. To investigate whole-plant responses to water shortages, we studied the growth of Tilia cordata Greenspire, a commonly planted urban tree, including development of its roots and stem diameter, leaf parameters and the harvested biomass. Our results showed that this cultivar was susceptible to drought and had reduced biomass in all three compartments: branch (30.7%), stem (16.7%) and coarse roots (45.2%). The decrease in the root:shoot ratio under drought suggested that more carbon was invested in the above-ground biomass. The development of fine roots and the loss of coarse root biomass showed that T. cordata Greenspire prioritised the growth of fine roots within the root system. The CityTree model’s simulation showed that the ability of this cultivar to provide ecosystem services, including cooling and CO2 fixation, was severely reduced. For use in harsh and dry urban environments, we recommend that urban managers take into account the capacity of trees to adapt to drought stress and provide sufficient rooting space, especially vertically, to help trees cope with drought.

Description: Research Highlights: When investigating the sorption of water on lignocellulosic materials, the sorption or mixing enthalpy is an interesting parameter that, together with the sorption isotherms commonly measured, can be used to characterize and understand the sorption process. We have compared different methods to assess these enthalpies. Additionally, we propose a sorption nomenclature. Background and Objectives: Sorption enthalpies are non-trivial to measure. We have, for the first time, measured sorption enthalpies on the same materials with four different methods, to be able to compare the method’s strengths and weaknesses. Materials and Methods: The following four methods were used on beech and Scots pine wood: isosteric heat, solution calorimetry, sorption calorimetry, and RH perfusion calorimetry. Results: The results for beech and pine were similar, and were in general agreement with the literature. We do not recommend one of the methods over the others, as they are quite different, and they can therefore be used to elucidate different aspects of the interactions between water and, for example, novel biobased materials (modified woods, cellulose derivatives, and regenerated cellulose).

Description: The aim of this study was to investigate the effect of season of the year on the frequency and degree of damage to residual trees caused during winter and summer timber harvesting operations in young alder stands. Analyses were conducted in pure black alder (Alnus glutinosa Gaertn.) stands aged 38 and 40 years, located in north-eastern Poland. Chainsaw logging was performed in the cut-to-length (CTL) system, while timber was extracted using an agricultural tractor with a trailer with manual timber loading and unloading. Damage caused in the stand as a result of early thinning operations was evaluated in terms of: (1) damage location, (2) wound size, (3) wound depth, and (4) the distance of the damaged tree from the skid trail. Timber harvesting caused damage in 8.3% of trees remaining in the stand. Both the total number of damaged trees (p = 0.001) and the number of trees damaged during felling (p = 0.01) and extraction of timber (p 〈 0.001) were greater in summer than in winter. Irrespective of the season, two-thirds of all cases of damage were caused during timber extraction, with 67.7% of damage recorded on trunks or root collars and 32.3% on roots. Irrespective of the season and the technological operation, slightly over 50% of cases of damage were small wounds of max. 10 cm2. The proportions of medium-sized wounds (11–100 cm2) and large wounds (over 100 cm2) were comparable. The majority of damaged trees (85.1%) were found in the vicinity (〈1 m) of the skid trails. The frequency of tree damage near the skid trail was twice as large in summer as in winter (p 〈 0.001).

Description: Forest health is an important aspect of sustainable forest management. The practical significance of health assessments of forest ecosystems is becoming more and more prominent because good knowledge about the health level of forests and the causes of unhealthy forests enables the identification of proper actions for enhancing sustainable development of forest ecosystems. This paper evaluated the health status of the forest ecosystem of Mount Taishan using the spatial analysis technique of GIS (Geographic Information System) and local forest inventory data. A comprehensive indicator system that reflects the health status of forestsin the study areawas established. Based on this indicator system, the health level of each sub-compartment of the forests in the study area was assessed. The results show that the high-quality grade forest (80.4 ha) and healthy grade forest (2671 ha) accounted for only 23.5% of the total forest area of Mount Taishan. About 60.5% of Mount Taishan forest was in a sub-health status. The area of unhealthy forests was 1865 ha (accounting for 16% of the total forest area), of which about 98 ha was inextremely unhealthy conditions.Asmore than two-thirds of the forests in Mount Taishan are in a sub-health or unhealthy state, effective measures for improving forest health are in urgent need in the study area.

Description: Forests play an important role in water and carbon cycles in semiarid regions such as the Mediterranean ecosystems. Previous research in the Chilean Mediterranean forests revealed a break point in 1980 in regional tree-ring chronologies linked to climate change. However, it is still unclear which populations and age classes are more affected by recent increases in drought conditions. In this study, we investigated the influence of recent variations in precipitation, temperature, and CO2 concentrations on tree growth of various populations and age classes of Nothofagus macrocarpa trees in Central Chile. We sampled 10 populations from five sites of N. macrocarpa through its whole geographic distribution in both Coastal and Andes ranges. We used standard dendrochronological methods to (i) group populations using principal component analysis, (ii) separate age classes (young, mature, and old trees), (iii) evaluate linear growth trends based on the basal area increment (BAI), and (iv) analyze the link between BAI and atmospheric changes using linear mixed-effects models. Results showed that young trees are more sensitive to climate variability. Regarding population grouping, we observed that all population clusters were sensitive to winter-spring precipitation, but only the Andes and Coastal populations were negatively correlated with temperature. The results of CO2 fertilization analyses were controversial and unclear. Since young trees from all population clusters reacted positively in the phase with an increase of atmospheric CO2 between 1980 and 2014, this behavior was not translated into growth for the last 15 years (2000–2014). However, it should be noted that the young trees of the highest elevation populations did not have a negative growth trend, so it seems that CO2 counteracted the negative effect of recent regional climate change (increase in temperature and precipitation decrease) in these population trees. Further studies are needed to assess the effects of climate variability over other ecological and physiological processes.

Description: Semanotus bifasciatus (Motschulsky) (Cerambycidae: Coleoptera) is a major forest borer in China, and attractants provide a promising method for the control of this pest. Exploration of the chemosensory mechanisms of S. bifasciatus is important for the development of efficient attractants for this pest. However, little information is available about the olfactory mechanisms of S. bifasciatus. Previous research has indicated that the trapping effects of the same attractant are different between Beijing and Shandong populations of S. bifasciatus. To explore the reasons for this, next-generation sequencing was performed to analyze the antennal transcriptome of both sexes of the two S. bifasciatus populations, and the olfactory-related genes were identified. Furthermore, the expression levels and single nucleotide polymorphisms (SNPs) of the olfactory-related genes between the two populations were compared. We found that the expression levels of odorant binding proteins (OBPs), odorant receptors (ORs), and sensory neuron membrane proteins (SNMPs) in male S. bifasciatus of the Beijing population were obviously lower than those in the Shandong population, and most of the conserved SNPs in OBPs and ORs of the two populations showed more diversity in the Beijing population. Our work provides a foundation for future research of the molecular olfactory mechanisms and pest management of S. bifasciatus, as well as other longhorn beetles.

Description: Research Highlights: The invasive species Pinus contorta, has become a new component of the vegetation in the Patagonian Steppe, invading the complex matrix of bare ground, tussock grasses and cushion plants. At a microsite scale, the type of native vegetation is one of the multiple factors determining the establishment of P. contorta and in some cases, increasing its survival, and as a result, the invasion of the species. Background and Objectives: The presence of trees, particularly pines, where they are naturally absent, represents a clear threat to Patagonian steppe ecosystems. Thus, understanding the interaction between pines and native plant species may be the key to understanding the invasion and applying management actions. Pinus contorta is considered one of the most aggressive among the pines species, with recognized impacts on regions in the southern hemisphere and Europe, and it is one of the main invaders in the Patagonian steppe. Our aim in this study is to determine the influence of surrounding vegetation on P. contorta spatial distribution, its establishment and future development in the Chilean Patagonia. Materials and Methods: We used point pattern analysis to determine the existence of spatial associations between P. contorta and the resident vegetation of the Patagonian steppe. Further, a seeding experiment was carried out to assess the influence of the surrounding vegetation during P. contorta establishment and first growing season. Results: We found that young P. contorta individuals are positively associated with the native cushion plant B. magellanica and also to tussock grasses. Seeding experiments showed that P. contorta is influenced by resident plant species during establishment and is able to establish on any ground cover type in the Patagonian steppe, but some resident plant species increase P. contorta survival chances. Conclusions: Pinus contorta is interacting with native vegetation and becoming an active component of the Patagonian steppe mosaic. Although pines can establish in any type of vegetation in the Patagonian steppe mosaics, native cushion and tussock grasses may be facilitating the process and promoting pine survival, allowing it to survive and thrive in this stressful environment.

Description: Egg recruitment quantifies the relative importance of realized fecundity and migration rates in the population dynamics of highly mobile insects. We develop here a formal context upon which to base the measurement and interpretation of egg recruitment in population dynamics of eastern and western spruce budworms, two geographically separated species that share a very similar ecology. Under most circumstances, per capita egg recruitment rates in these budworms are higher in low-density populations and lower in high-density populations, relative to the regional mean: Low-density populations are nearly always migration sinks for gravid moths, and dense populations nearly always sources. The slope of this relationship, measured on a log scale, is negatively correlated with migration rate, and ranges between 0 and −1. The steeper the slope, the more marked net migration. Using our western spruce budworm observations, we found strong evidence of density-dependent emigration in budworms, so migration is not simply a random perturbation in the lagged, density-dependent stochastic process leading to budworm outbreaks. It is itself statistically and biologically density-dependent. Therefore, moth migration is a synchronizing factor and a spread mechanism that is essential to understanding the development and expansion of spruce budworm outbreaks at regional scales in the boreal forests of North America.

Description: Subtropical forests have great potential as carbon sinks; however, the relationship between net ecosystem productivity (NEP) and climate change is still unclear. This study took Zhejiang Province, a subtropical region, as an example. Based on remote sensing classification data of forest resources, the integrated terrestrial ecosystem carbon cycle (InTEC) model was used to simulate the spatiotemporal dynamics of the forest NEP in Zhejiang Province during 1985–2015 and analyze its response to meteorological factors such as temperature, precipitation, relative humidity, and radiation. Three patterns emerged: (1) The optimized InTEC model can better simulate the forest NEP in Zhejiang Province, and the correlation coefficient between the simulated NEP and observed NEP was up to 0.75. (2) From 1985 to 2015, the increase in the total NEP was rapid, with an average annual growth rate of 1.52 Tg·C·yr−1. During 1985–1988, the forests in Zhejiang Province were carbon sources. After 1988, the forests turned into carbon sinks and this continued to increase. During 2000–2015, more than 97% of the forests in Zhejiang Province were carbon sinks. The total NEP reached 32.02 Tg·C·yr−1, and the annual mean NEP increased to 441.91 gC·m−2·yr−1. The carbon sequestration capacity of forests in the east and southwest of Zhejiang Province is higher than that in the northeast of Zhejiang Province. (3) From 2000 to 2015, there was an extremely significant correlation between forest NEP and precipitation, with a correlation coefficient of 0.85. Simultaneously, the forest NEP showed a negative correlation with temperature and radiation, with a correlation coefficient of −0.56 for both, and the forest NEP was slightly negatively correlated with relative humidity. The relative contribution rates of temperature, precipitation, relative humidity, and radiation data to NEP showed that the contribution of precipitation to NEP is the largest, reaching 61%, followed by temperature and radiation at 18% and 17%, respectively. The relative contribution rate of relative humidity is the smallest at only 4%. During the period of 1985–1999, due to significant man-made disturbances, the NEP had a weak correlation with temperature, precipitation, relative humidity, and radiation. The results of this study are important for addressing climate change and illustrating the response mechanism between subtropical forest NEP and climate change.

Description: Water vapor sorption is the most fundamental aspect of wood-moisture relations. It is directly or indirectly related to the physical properties of wood and the onset of wood-damage mechanisms. While sorption properties of cellulosic materials have been utilized since antiquity, the time-dependent transition from one moisture content to another (i.e., sorption kinetics) has received much less attention. In this critical review, we present the state-of-the-art of water vapor sorption kinetics in wood. We first examine different experimental methods that have been used to measure sorption kinetics, from the quartz helix vacuum balance beginning in earnest in the 1930s, to automated sorption balances used recently. We then give an overview of experimental observations and describe the physical phenomena that occur during the sorption process, which potentially govern the following kinetics: boundary layer mass transfer resistance, heat of sorption, cell wall diffusion, swelling, and polymer mobility. Finally, we evaluate theoretical models that have been proposed for describing sorption kinetics, considering both experimental data and the physical processes described in the previous section. It is clear that no previously developed model can phenomenologically describe the sorption process. Instead, new models are needed. We conclude that the development of new models will require more than simple gravimetric measurements. In addition to mass changes, complementary techniques are needed to probe other important physical quantities on multiple length scales.

Description: Urban tree growth is often affected by reduced water availability, higher temperatures, small and compacted planting pits, as well as high nutrient and pollution inputs. Despite these hindering growth conditions, recent studies found a surprisingly better growth of urban trees compared to trees at rural sites, and an enhanced growth of trees in recent times. We compared urban versus rural growing Sakhalin fir (Abies sachalinensis (F. Schmidt) Mast.) trees in Sapporo, northern Japan and analyzed the growth differences between growing sites and the effects of environmental pollution (NO2, NOX, SO2 and OX) on tree growth. Tree growth was assessed by a dendrochronological study across a gradient from urban to rural sites and related to high detailed environmental pollution data with mixed model approaches and regression analyses. A higher growth of urban trees compared to rural trees was found, along with an overall accelerated growth rate of A. sachalinensis trees over time. Moreover, environmental pollution seems to positively affect tree growth, though with the exception of oxides OX which had strong negative correlations with growth. In conclusion, higher temperatures, changed soil nutrient status, higher risks of water-logging, increased oxide concentrations, as well as higher age negatively affected the growth of rural trees. The future growth of urban A. sachalinensis will provide more insights as to whether the results were induced by environmental pollution and climate or biased on a higher age of rural trees. Nevertheless, the results clearly indicate that environmental pollution, especially in terms of NO2 and NOX poses no threat to urban tree growth in Sapporo.

Description: Forest landscape plays a critical role in the resource management and recreational planning of forest destinations. An assessment of forest landscape quality (FLQ) could reflect the distribution of landscape resources, hence identifying the hotpots and areas with high visual quality and protection values. The objective of this study is to propose, for the first time, a methodology for assessing FLQ at the national level. Based on China’s forestry inventory database, the paper identified landform patterns and vegetative patterns as determinants (including 12 indicators) to establish an evaluation index system, and further implemented and mapped FLQ using the ArcGIS Engine platform. Results show high mountain ranges and tropical areas in China often have a high-quality forest landscape, while low FLQ scores are found in low mountains and foothills. The distribution of the four FLQ levels indicates most forest areas are featured with mediocre- or low- quality landscape values, and the differences of FLQ among different forest types are obvious. Furthermore, there is a relatively low correlation between the total forest area and the area of high-quality forest landscape. Overall, this study could contribute to enriching the existing assessment system for FLQ and to guiding the planning, policy development, and decision-making for China’s forestry administration.

Description: This study analyzes the impact of the Indonesian government’s decentralization policy and movement on the state forest community involvement program on the island of Java from 2000 to 2014. For more than 30 years, approximately 76 percent of the state-owned forests in Java, covering 2.4 mio. hectares (5.9 mio. acres or 9,266 square miles), or 42 percent of the forests in Java, were exclusively managed by the State Forest Company (SFC). The general perception was, and largely still is, that the decentralization era reforms had little or no effects in Java, simply because the national decentralization regulations essentially maintained this dominant role. Thus, possible effects were hardly examined. This study aims to fill this gap by analyzing whether the national decentralization movement and policy affected and changed the policies and practices of involving local actors and communities in Java’s state forest governance and management. Our analytical framework follows the Policy Arrangement Approach (PAA), comprising four main analytical dimensions for assessing change and stability: The actors involved; the distribution of power and resources; the existing and applied rules; and discourses concerning the topic of research. The analysis is based on an extensive review of scholarly literature and policy documents, as well as 73 in-depth interviews with actors, from the national to the local levels, of two districts in Central Java. Overall, our findings show that the nationwide decentralization movement significantly affects the modes of collaborative forest management in Java, much more than one would expect if only looking at changes in the respective legal texts. The movement fueled changes, inter alia, by promoting discourses on sustainable forest management for local development, and triggering the revival of grassroots movements further empowered by local non-governmental organizations (NGOs), as well as by triggering the formal institutionalization of the informal norms and practices of local communities. New actors entered the arena, and the distribution of power, resources and benefits drawn from state forest management has changed in favor of district authorities and local communities at the expense of the State Forest Company.

Description: Forests are part of a complex landscape mosaic and play a crucial role for people living both in rural and urbanized spaces. Recent progresses in modelling and Decision Support System (DSS) applied to the forestry sector promise to improve public participative forest management and decision-making in planning and conservation issues. However, most DSS are not open-source systems, being in many cases software designed for site-specific applications in forest ecosystems. Furthermore, some of these systems often miss challenging the integration of other land uses within the landscape matrix, which is a key issue in modern forestry planning aiming at linking recent developments in open-source Spatial-DSS systems to sectorial forest knowledge. This paper aims at demonstrating that a new type of S-DSS, developed within the Life+ project SOILCONSWEB over an open-source Geospatial Cyber-Infrastructure (GCI) platform, can provide a strategic web-based operational tool for forest resources management and multi-purpose planning. In order to perform simulation modelling, all accessible via the Web, the GCI platform supports acquisition and processing of both static and dynamic data (e.g., spatial distribution of soil and forest types, growing stock and yield), data visualization and computer on-the-fly applications. The DSS forestry tool has been applied to a forest area of 5,574 ha in the southern Apennines of Peninsular Italy, and it has been designed to address forest knowledge and management providing operational support to private forest owners and decision-makers involved in management of forest landscape at different levels. Such a geospatial S-DSS tool for supporting integrated forest knowledge at landscape represents a promising tool to implement sustainable forest management and planning. Results and output of the platform will be shown through a short selection of practical case studies.

Description: Both leaf area (LA) and leaf angle distribution are the most important eco-physiological measures of tree crowns. However, there are limited published investigations on the two parameters of Moso bamboo (Phyllostachys edulis (Carrière) J. Houz., abbreviated as MB). The aim of this study was to develop allometric equations for predicting crown LA of MB by taking the diameter at breast height (DBH) and tree height (H) as predictors and to investigate the leaf angle distribution of a MB crown based on direct leaf angle measurements. Data were destructively sampled from 29 MB crowns including DBH, H, biomass and the area of sampled leaves, biomass of total crown leaves, and leaf angles. The results indicate that (1) the specific leaf area (SLA) of a MB crown decreases from the bottom to the top; (2) the vertical LA distribution of MB crowns follow a “Muffin top” shape; (3) the LA of MB crowns show large variations, from 7.42 to 74.38 m2; (4) both DBH and H are good predictors in allometry-based LA estimations for a MB crown; (5) linear, exponential, and logarithmic regressions show similar capabilities for the LA estimations; (6) leaf angle distributions from the top to the bottom of a MB crown can be considered as invariant; and (7) the leaf angle distribution of a MB crown is close to the planophile case. The results provide an important tool to estimate the LA of MB on the standing scale based on DBH or H measurements, provide useful prior knowledge for extracting leaf area indexes of MB canopies from remote sensing-based observations, and, therefore, will potentially serve as a crucial reference for calculating carbon balances and other ecological studies of MB forests.

Description: Understanding how environmental changes driven by urbanization impact the biodiversity in urban riparian areas has great importance for landscape planning and river ecosystem conservation. There have been many studies on the response of bird communities to different environmental variables in urban parks; however, although supporting some of the highest bird diversities, case studies in urban riparian areas remain limited. In existing research, few studies have considered the impact of both local waterfront characteristics and surrounding environmental variables at a larger scale. In this study, we selected birds as the indicator to clarify their response to both local- and landscape-scale environmental variables in riparian areas of Tsing river, Beijing, in terms of (a) vegetation composition, (b) human disturbance, (c) land cover, and (d) landscape connectivity. We hypothesized that birds with different biological characteristics may respond differently to environmental variables. Birds were then further grouped according to the habitat type, residential type, and feeding type. It turned out that the coverage of grass and the disturbance of pedestrians are the most influential variables. Besides, compared with the land cover and landscape connectivity, the total contribution of vegetation characteristics and human disturbance accounts for the main proportion of explained variance. Information pertaining to these environmental variables can provide evidence to support bird conservation efforts in urban areas, and the identified distance threshold provides a basis for future landscape connectivity assessments.

Description: In this study, 145 poplar (Populus × euramericana cv.’74/76’) seedlings, a common plantation tree species in China, were selected and their ultrasonic velocities were measured at four timepoints during the first growth year. After that, 60 poplar seedlings were randomly selected and cut down to determine their acoustic velocity, using the acoustic resonance method. The effects of influencing factors such as wood green density, microfibril angle, growth days, and root-collar diameter on acoustic speed in seedlings and the relationship between ultrasonic speed and acoustic resonance speed were investigated and analyzed in this work. The number of specimens used for investigating growth days and root-collar diameter was 145 in both cases, while 60 and two specimens were used for investigating wood density and the microfibril angle, respectively. The results of this study showed that the ultrasonic speed of poplar seedlings significantly and linearly increased with growth days, within 209 growing days. The ultrasonic velocity of poplar seedlings has a high and positive correlation with growth days, and the correlation was 0.99. However, no significant relationship was found between the ultrasonic velocity and root-collar diameter of poplar seedlings. Furthermore, a low and negative relationship was found between wood density and ultrasonic speed (R2 = 0.26). However, ultrasonic velocity significantly decreased with increasing microfibril angle (MFA) in two seedlings, and thus MFA may have an impact on ultrasonic speed in poplar seedlings. In addition, ultrasonic velocity was found to have a strong correlation with acoustic resonance velocity (R2 = 0.81) and a good correlation, R2 = 0.75, was also found between the dynamic moduli of elasticity from ultrasonic and acoustic resonance tests. The results of this study indicate that the ultrasonic technique can possibly be used to measure the ultrasound speed of young seedlings, and thus early screen seedlings for their stiffness properties in the future.

Description: Research Highlights: We find that biochar plus fertilizer has synergistic and positive effects on seedling growth and robustness, but slightly lowers early seedling survival. Biochar plus fertilizer has the potential to greatly decrease costs associated with afforestation as compared to traditional fertilization and gives better results. Background and Objectives: Biochar can improve soil fertility and plant yield in crops. However, there is little information regarding the effects of biochar on trees, even though reforestation/afforestation projects are increasing and are often unsuccessful due to soil fertility limitations. This study aims to increase knowledge of biochar use as a reforestation tool. Materials and Methods: We measured survival and growth in an early ((Guazuma crinita Mart. [n = 240])) and a late (Terminalia amazonia (J.F. Gmel.) Exell. [n = 240]) successional species under 6 different biochar treatments in a 6-month nursery experiment. Results: (i) Survival was highest in the 1 t/ha biochar treatment, while treatments containing fertilizers or biochar at 5 t/ha lowered the survival rate of both species compared to the control; (ii) simultaneous addition of biochar and fertilizer lead to significant increases in height, diameter, total number of leaves, and aboveground and belowground biomass of both species as compared to other treatments; (iii) biochar treatment containing 1 t/ha with and without fertilizer showed significantly better results than applications of 5 t/ha; and (iv) Guazuma crinita responded more strongly to treatments containing biochar and fertilizers compared to Terminalia amazonia, which is suggestive of greater synergetic effects of biochar and fertilizer addition on early successional tree species. Conclusions: Applying biochar and fertilizer is synergistic and outperforms any single treatment, as well as the control, in terms of plant performance. This case study suggests that biochar can greatly improve reforestation/afforestation projects by increasing plant performance while substantially reducing fertilizer and labor maintenance costs. Field experiments and testing of additional species is needed to generalize the findings.

Description: Studying how cambial age and axial height affects wood anatomical traits may improve our understanding of xylem hydraulics, heartwood formation and axial growth. Radial strips were collected from six different heights (0–11.3 m) along the main trunk of three Manchurian catalpa (Catalpa bungei) trees, yielding 88 samples. In total, thirteen wood anatomical vessel and fiber traits were observed usinglight microscopy (LM) and scanning electron microscopy (SEM), and linear models were used to analyse the combined effect of axial height, cambial age and their interaction. Vessel diameter differed by about one order of magnitude between early- and latewood, and increased significantly with both cambial age and axial height in latewood, while it was positively affected by cambial age and independent of height in earlywood. Vertical position further had a positive effect on earlywood vessel density, and negative effects on fibre wall thickness, wall thickness to diameter ratio and length. Cambial age had positive effects on the pit membrane diameter and vessel element length, while the annual diameter growth decreased with both cambial age and axial position. In contrast, early- and latewood fiber diameter were unaffected by both cambial age and axial height. We further observed an increasing amount of tyloses from sapwood to heartwood, accompanied by an increase of warty layers and amorphous deposits on cell walls, bordered pit membranes and pit apertures. This study highlights the significant effects of cambial age and vertical position on xylem anatomical traits, and confirms earlier work that cautions to take into account xylem spatial position when interpreting wood anatomical structures, and thus, xylem hydraulic functioning.

Description: Understanding the contribution of forest ecosystems to regulating greenhouse gas emissions and maintaining the atmospheric CO2 balance requires the accurate quantification of above-ground biomass (AGB) at the individual tree species level. The main objective of this study was to develop species-specific allometric equations for the total AGB and various biomass components, including stem, branch, and foliage biomass in Khangai region, northern Mongolia. We destructively sampled a total of 183 trees of five species (22–74 trees per species), including Siberian stone pine (Pinus sibirica Du Tour.), Asian white birch (Betula platyphylla Sukacz.), Mongolian poplar (Populus suaveolens Fisch.), Siberian spruce (Picea obovata Ldb.), and Siberian larch (Larix sibirica Ldb.), across this region. The results showed that for the five species, the average biomass proportion for the stems was 75%, followed by branches at 20% and foliage at 5%. The species-specific component and total AGB models for the Khangai region were developed using tree diameter at breast height (D) and D² and tree height (H) combined ( D 2 H ); and both D and H were used as independent variables. The best allometric model was lnŶ = lna + b × lnD + c × lnH for the various components and total AGB of B. platyphylla and L. sibirica, for the stems and total AGB of P. suaveolens, and for the stem and branch biomass of P. obovata. The equation lnŶ = lna + b × ln( D 2 × H ) was best for the various components and total AGB of P. sibirica, for the branch and foliage biomass of P. suaveolens, and for AGB of P. obovata. The equation lnŶ = lna + b × ln(D) was best only for the foliage biomass of P. obovata. Our results highlight that developing species-specific tree AGB models is very important for accurately estimating the biomass in the Khangai forest region of Mongolia. Our biomass models will be used at the tree level inventories with sample plots in the Khangai forest region.

Description: The accurate estimation of leaf area is of great importance for the acquisition of information on the forest canopy structure. Currently, direct harvesting is used to obtain leaf area; however, it is difficult to quickly and effectively extract the leaf area of a forest. Although remote sensing technology can obtain leaf area by using a wide range of leaf area estimates, such technology cannot accurately estimate leaf area at small spatial scales. The purpose of this study is to examine the use of terrestrial laser scanning data to achieve a fast, accurate, and non-destructive estimation of individual tree leaf area. We use terrestrial laser scanning data to obtain 3D point cloud data for individual tree canopies of Pinus massoniana. Using voxel conversion, we develop a model for the number of voxels and canopy leaf area and then apply it to the 3D data. The results show significant positive correlations between reference leaf area and mass (R2 = 0.8603; p 〈 0.01). Our findings demonstrate that using terrestrial laser point cloud data with a layer thickness of 0.1 m and voxel size of 0.05 m can effectively improve leaf area estimations. We verify the suitability of the voxel-based method for estimating the leaf area of P. massoniana and confirmed the effectiveness of this non-destructive method.

Description: Research Highlights: This study was the first to use metabolomics techniques to investigate seed germination in poplar, a model woody plant. Our results lay a foundation for uncovering changes in metabolite levels during woody plant seed germination and for understanding the underlying mechanism. Background and Objectives: Poplar is a model woody plant. Because poplar can be easily propagated asexually, the molecular mechanism of poplar seed germination has not been well studied. However, long-term asexual reproduction of poplar leads to seedlings with weak resistance, high vulnerability to degradation, and reduced growth potential. Materials and Methods: The non-targeted metabolomics technique was used to analyze changing trends in metabolite contents during the poplar seed germination process. Results: We found that the number of differential metabolites increased as seed germination progressed. Metabolic pathway analysis of differential metabolites revealed that galactose metabolism and alanine, aspartate, and glutamate metabolism were significantly enriched during all germination periods. MapMan-based visual analysis of metabolic pathways of differential metabolites indicated that glutamine, glutamic acid, phenylalanine, arginine, and asparagine contents increased with germination time, with most metabolites related to glucose metabolism following similar trends. Contents of most metabolites related to the tricarboxylic acid cycle exhibited a fluctuating pattern. Conclusions: This study has revealed the major changes taking place in primary metabolite contents during poplar seed germination and has laid the foundation for elucidation of the molecular mechanism of poplar seed germination.

Description: Improving low-efficiency artificial forests represents a popular forest hydrological issue, and exploring the optimal stand density (OSD) of low-efficiency artificial forests is an effective method for improving the soil conditions of forestland to prevent the deterioration of ecological function. Water and nutrients were the main limiting factors for vegetation growth. However, relatively few studies addressed the optimization of stand density based on these two factors at the same time. In this study, a total of 176 standard plots (20 × 20 m2) with six stand densities (~500, ~1000, ~1500, ~2000, ~2500 and ~3000 plants·hm−2) were established to investigate the water resources (soil moisture content (SMC), soil evaporation rate (SER), and vegetation transpiration rate (VTR)) and soil nutrient resources (total nitrogen (TN), total phosphorus (TP), total potassium (TK), calcium (CaCO3), organic matter content (OMC)) in low-efficiency Robinia pseudoacacia forestland on the Loess Plateau in western Shanxi, China from June to September each year from 2017 to 2018. The relationships between stand density and water and nutrient resources were analysed with the response surface method (RSM). The RSM results indicated that the OSD averaged 1594 plants·hm−2 and ranged from 940 to 2386 plants·hm−2. The percentage of standard plots with an unreasonable stand density was 35.29%, and 65% of these plots had a value that was higher than the maximum in the range while 35% had a value that was lower than the minimum. These results indicate that the current stand density should be manipulated to fall within the identified OSD range to ensure the normal functions of soil and water conservation in R. pseudoacacia forests. The results of this study serve as a guide for optimizing the stand density of low-efficiency R. pseudoacacia forests in China.

Description: The earthquake and tsunami on 11 March 2011 led to a meltdown followed by a hydrogen explosion at the Fukushima–Daiichi nuclear power plant in Japan, causing the dispersal of abundant radionuclides into the atmosphere and ocean. The radionuclides were deposited onto trees and local residences in aerosol or gaseous forms that were partly absorbed by rain or melting snow. Here, we show that the radionuclides attached to the surfaces of trees, in which some radiocesium was incorporated into the xylem through ray cells and through symplastic pathways. The level of incorporated radiocesium varied based on tree species and age because of the ability of radiocesium to attach to the surface of the outer bark. After four years, the radiocesium level in the forest has been decreasing as it is washed out with rainwater into the sea and as it decays over time due to its half-life, but it can also be continuously recycled through leaf tissue, litter, mulch, and soil. As a result, the level of radiocesium was relatively increased in the heartwood and roots of trees at four years after the event. In private forest fields, most trees were left as afforested trees without being used for timber, although some trees were cut down. We discuss an interdisciplinary field study on the immediate effects of high radiation levels upon afforested trees in private forest fields.

Description: Research Highlights: In this study, we found different regulatory mechanisms in two contrasting provenances of Norway spruce responding to progressive drought stress. Background and Objectives: In the context of climate and environmental changes, the following question of high importance in scientific studies is: How will Norway spruce, which forms a dominant component in European mountain and boreal forests, be able to cope with the increasing frequencies and intensities of drought periods? The aim of the study was to investigate the physiological responses of eight-month-old seedlings, as a most vulnerable phase of forest tree life, for different spruce provenances, to find out variable strategies in relation to controlled drought stress. Materials and Methods: We performed an experiment under controlled conditions with spruce seedlings from a climatologically warmer stand of 410 m above sea level (a.s.l.), and a moderately cool stand of 931 m a.s.l. The soil water content, leaf predawn water potential, the osmotic potential of needles, the relative water content of needles, and the photosynthetic performance and the contents of primary metabolites (proline and abscisic acid) were investigated as indicators of the spruce seedlings’ responses to water stress. The status of the indicators was analyzed at different temporal intervals, using repeated measures analysis of variance (ANOVA), reflecting the seedlings’ water conditions (early drought, drought, or recovery). On a daily basis, the differences among the indicators were tested with one-way ANOVA. A principal component analysis (PCA) was used to identify the provenance-specific responses of tree drought indicators in a multi-dimensional data set. Results: The responses to drought stress differed between the provenances. Whereas seedlings of ‘warm’ provenance from a low altitude performed a conservation strategy, with high amounts of accumulated abscisic acid and closing the stomata faster, the reactions of ‘cool’ provenance seedlings from a higher altitude were not so sensitive and the plants’ water supply and photosynthetic performance remained significantly higher. These findings indicate that a higher drought resistance in ‘cool’ provenance could be related to greater amounts of proline amino-acids, which are accumulated from the beginning of the drought simulation. Furthermore, proline accumulation resulting in increased stress tolerance is controlled through another mechanism than osmotic adjustment. Conclusions: The observed variations in the regulatory mechanisms used to develop adaptive strategies in different provenances are an important factor for seedling survival under a changing climate.

Description: Forests are important in the global carbon cycle and it is necessary to quickly and accurately measure forest volume to estimate forest aboveground biomass (AGB) and aboveground carbon storage (AGC). In this paper, we used data from the eighth forest resources inventory of China to establish two stand volume models based on stand density and forest basal area for 37 arbor forest types (dominant species); and performed a comparative analysis to obtain the best model. Then the AGB, AGB density, AGC, and AGC density of the different forest types and regions were estimated by conversion function methods. The results showed that: (1) The volume model of tree height and forest basal area could better fit the natural growth process of forests, and 36 of the 37 forest types had R2 greater than 0.8; (2) The average AGB density of arbor forest in China was 95.03 Mg ha−1 and the average AGC density was 48.15 Mg ha−1 (3) Among forest types, Picea asperata Mast., Quercus spp., and Populus spp. had the highest AGB and AGC, while Cinnamomum camphora (L.) Presl, Pinus taiwanensis Hayata, and Pinus densiflora Sieb. et Zucc. had the lowest. The AGB density and AGC density of Phoebe zhennan S. Lee et F. N. Wei and Pinus densata Mast. were the highest, while those of Pinus densiflora Sieb. et Zucc., Pinus elliottii Engelmann, and Eucalyptus robusta Smith were the lowest. (4) Among regions, AGB and AGC ranging from high to low, were as follows: northwest, southwest, northeast, central south, east, and north. The northwest and southwest regions accounted for more than 70% of the country’s AGB and AGC. The average AGB density and AGC density among the regions were 91.34 Mg ha−1 and 46.4 Mg ha−1, respectively. Ranging from high to low as follows: southwest, northwest, northeast, east, central south, and north. The methods used in this paper provide a basis for fast and accurate estimation of stand volume, and the estimates of AGB and AGC have important reference value for explaining the role of ecosystems in coping with global climate change in China.

Description: In recent decades, the concept of forest certification under the Forest Stewardship Council (FSC) has been widely adopted in selected Southeast European countries (Bosnia and Herzegovina, Croatia, Serbia, and Slovenia). As sustainability is traditionally recognised as a leading principle in the forest management doctrine in these countries, the aim of this study was to understand whether, and how, FSC forest certification contributes to the sustainable management of state forests. The research was carried out in two phases. First, in order to assess forest management compliance with FSC standard, non-conformities for the period 2014–2018, identified in audit Public Summary Reports, were analysed in all public companies that managed state-owned forests in selected countries. Further, in-depth, semi-structured interviews with the professionals responsible for forest certification in these companies were conducted (n = 11) to determine the contribution of forest certification to the economic, ecological, and social aspects of sustainable forest management. In total, 185 non-conformities were analysed. The results showed that FSC certification was successful in addressing certain problems in forest management practices and contributed to sustainable forest management, mainly covering social and ecological issues. The most frequently identified non-conformities were those related to FSC Principle 4 Community relations and worker’s rights (32.3% of all non-conformities) and Principle 6 Environmental impact (30.4% of all non-conformities). The contribution of FSC certification to sustainable forest management is mainly reflected in the following aspects: Worker’s rights; health and safety of employees; availability of appropriate personal protective equipment; consultation with local people and interest groups; awareness of environmental impacts of forestry operations; waste disposal and storage of fuel; improving the image of forest companies and maintenance of high-conservation-value forests. The majority of non-conformities were minor and required procedural changes to be closed. Moreover, there are no statistically significant differences between the countries with regard to the number of non-conformities for all principles. It can be concluded that FSC certification, as a market-driven mechanism, plays an important role by influencing forest management practices and business operations of public forest companies in a positive manner.

Description: In this study, Japanese cedar (Cryptomeria japonica (L. f.) D. Don) harvested from a plantation in Taiwan was used to develop built-up beams using self-tapping screws as metal connectors and resorcinol formaldehyde resin as glue to assemble components based on various assembly configurations. Results showed that adding glue provided flexural rigidity, whereas assembly using self-tapping screws resulted in built-up beams with high ductility but relatively low flexural bearing capacity. Beams used glue exhibited approximately linear behavior, whereas those using only screws exhibited some undulating and stepwise responses, implying that shear force between the flanges and the web may cause buckling as well as the dislocation of the self-tapping screws. When using components of similar grades, adding another web can improve the performance. Furthermore, the grades of flanges can strongly influence the flexural load-bearing capacity. In addition, a smaller spacing between the screws can improve the flexural load-bearing performance, but also cause wooden components to crack. Typical bending failure modes were observed in the developed built-up beams, indicating tension failure of the bottom flange as well as slippage between flanges and the web due to horizontal shear, which also caused buckling deformations in the screws.

Description: Pine pitch canker (PPC), caused by the pathogenic fungus Fusarium circinatum (Nirenberg and O’ Donnell), is a serious threat to pine forests globally. The recent introduction of the pathogen to Southern Europe and its spread in Mediterranean region is alarming considering the immense ecological and economic importance of pines in the region. Pines in forests and nurseries can be infected, resulting in severe growth losses and mortality. The pathogen is known to spread in plants for planting and in seeds, and results from recent studies have indicated that F. circinatum may also spread through phoretic associations with certain insects. With this review, we aim to expand the current understanding of the risk of insect-mediated spread of PPC in different parts of Europe. Through the joint action of a multinational researcher team, we collate the existing information about the insect species spectrum in different biogeographic conditions and scrutinize the potential of these insects to transmit F. circinatum spores in forests and nurseries. We also discuss the impact of environmental factors and forest management in this context. We present evidence for the existence of a high diversity of insects with potential to weaken pines and disseminate PPC in Europe, including several common beetle species. In many parts of Europe, temperatures are projected to rise, which may promote the activity of several insect species, supporting multivoltinism and thus, further amplifying the risk of insect-mediated dissemination of PPC. Integrated pest management (IPM) solutions that comply with forest management practices need to be developed to reduce this risk. We recommend careful monitoring of insect populations as the basis for successful IPM. Improved understanding of environmental control of the interaction between insects, the pathogen, and host trees is needed in order to support development of bio-rational strategies to safeguard European pine trees and forests against F. circinatum in future.

Description: RING-H2 genes, the most abundant RING-type genes encoding putative ubiquitin ligase E3, are involved in diverse biological processes. Whether RING-H2 genes are related to wood formation remains to be identified in trees. In this study, we identified 288 RING-H2 genes in Populus trichocarpa, and found that the segmental and tandem duplication events contributed to RING-H2 gene expansion. Microarray dataset (from Affymetrix poplar genome arrays) showed that 64 of the 249 RING-H2 genes were highly or preferentially expressed in stem xylem. According to the AspWood RNAseq dataset, the transcription levels of genes PtrRHH21, 33, 48, 69, 88, 93, 94, 121, 141, 166, 175, 192, 208, 214, 250 and 257 were significantly increased in the xylem ranging from the expanding xylem to the lignifying xylem, suggesting their association with wood formation. Promoter analyses revealed that most of the preferentially xylem-expressed RING-H2 genes possessed SNBE, TERE, M46RE, AC and SMRE cis-elements, which are involved in secondary cell wall biosynthesis and programmed cell death. Based on the promoter GUS-based analysis result, PtrRHH94 was indicated to be associated with wood formation in transgenic P. trichocarpa. Taken together, dozens of Populus RING-H2 gene candidates associated with wood formation have been identified based on multiple gene expression analyses.

Description: Understanding the occurrence patterns of forest pests is fundamental for effective forest management from both economic and ecological perspectives. Here, we review the history of the occurrence patterns and causes of outbreaks and declines of pests in Korean pine forests over the last 50 years. During this period, the major pests of pine forests in Korea have shifted from pine caterpillar (Dendrolimus spectabilis Butler) to the pine needle gall midge (PNGM, Thecodiplosis japonensis (Uchida and Inouye)) and finally to pine wilt disease (PWD) caused by the pine wood nematode (Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle). Outbreaks of pine caterpillar, a native species in Korea, have been recorded as far back as 900 years, and it was the most relevant forest pest in Korea until the 1970s. The decline of its importance has been attributed to reforestation and higher levels of subsequent natural enemy activity. The PNGM is an invasive species, first discovered in Korea in 1929, that became widely distributed by 1992 and the major forest pest in the 1980s and 1990s. A suite of parasitic wasps attacking the PNGM contributed at least partially to the decline of PNGM densities. Following the decline of the PNGM, damage from PWD has increased since 2003. These shifts in major forest pests might be related to changes in forest composition and interactions among forest pests. Therefore, a new management strategy for controlling forest pests is required to mitigate the decline of pine forests in Korea.

Description: Research Highlights: Sequence phylogeny, genome organisation, gene structure, conserved motifs, promoter cis-element and expression profiling of poplar NACs related to salt stress were detected. In addition, expression of two salt-induced NACs was analysed. Background and Objectives: NAC transcription factor (TF) proteins are involved in a wide range of functions during plant development and stress-related endurance processes. To understand the function of Populus NAC TFs in salt stress tolerance, we characterised the structure and expression profile of a total of 289 NAC members. Materials and Methods: Sequence phylogeny, genome organisation, gene structure, motif composition and promoter cis-element were detected using bioinformatics. The expression pattern of Populus NAC TFs under salt stress was also detected using RNA-Seq and RT-qPCR. Results: Synteny analysis showed that 46 and 37 Populus NAC genes were involved in whole-genome duplication and tandem duplication events, respectively. The expression pattern of Populus NAC TFs under salt stress showed the expression of the 289 PtNACs of 84K poplar was induced. Similar expression trends of NACs were found in Populus simonii × P. nigra T. S. Hwang et Liang and Arabidopsis thaliana (L.) Heynh. Conclusions: The correlation analysis showed that the expression of two differentially expressed NAC genes PtNAC024 and PtNAC182 was significantly associated with most of the 63 differentially expressed genes tested. The expression of PtNAC024 and PtNAC182 in different tissues was also analysed in silico and different expression patterns were found. Together, this study provides a solid basis to explore stress-related NAC TF functions in Populus salt tolerance and development.

Description: Cable yarding is the most commonly used technique for harvesting timber from steep terrain in central Europe. During the planning process, one important task is to define the cable road layout. This means that the harvesting technology and cable road location must be specified for a given timber parcel. Although managers must minimize harvesting costs, it is even more important that such work on forests reduces the potential for damage to the residual stand and ensures that environmental conditions remain suitable for regeneration. However, current methods are geared only toward minimizing harvesting costs and are computationally demanding and difficult to handle for the end user. These limitations hinder broad application of such methods. Further, the underlying productivity models used for cost estimation do not cover all conditions of an area and they cannot be applied over a whole harvesting area. To overcome these shortcomings, we present: (1) a multiobjective optimization approach that leads to realistic, practicable results that consider multiple conflicting design objectives, and (2) a concept for an easy-to-use application. We compare the practical applicability and performance of the results achieved with multiobjective optimization with those achieved with single-objective (cost-minimal) optimization. Based on these points, we then present and discuss a concept for a user-friendly implementation. The model was tested on two sites in Switzerland. The study produced the following major findings: (1) Single-objective alternatives have no practical relevance, whereas multiobjective alternatives are preferable in real-world applications and lead to realistic solutions; (2) the solution process for a planning unit should include analysis of the Pareto frontier; and (3) results can only be made available within a useful period of time by parallelizing computing operations.

Description: Salt-affected soils in the Northern Great Plains, USA, can impact the long-term survival and growth of trees recommended for agroforestry systems, with Russian olive (Elaeagnus angustifolia L.) being one of few options that survives on these sites. Similarly, hybrid poplars have been used for phytotechnologies on high-salinity soils throughout the world. The objective of this study was to test the survival, height growth, and phytoextraction potential of eight hybrid poplar clones (Populus deltoides Bartr. ex Marsh. × P. nigra L. ‘Robusta’, ‘DN17’, ‘DN182’, ‘DN5’; P. deltoides × P. maximowiczii A. Henry ‘NC14104’, ‘NC14106’; P. nigra × P. maximowiczii ‘NM2’, ‘NM6’) versus Russian olive grown on soils categorized according to initial salinity levels: low (0.1 to 3.9 dS m−1), medium (4.0 to 5.9 dS m−1), and high (6.0 to 10.0 dS m−1). Seven trees per genotype were grown in each salinity treatment at a spacing of 3 × 3 m for four years in Burleigh County, North Dakota. Survival and height were determined following the first four growing seasons, and leaf phytoextraction potential of Al, Ca, Cd, Fe, K, Mg, Mn, Na, and Zn was measured for one-year-old trees. Soil salinity decreased over time, reflecting the phytoextraction potential of the trees. Russian olive did not survive as well as expected, having lower overall survival than three of the hybrid poplar clones (‘DN17’, ‘DN5’, ‘NM6’). At the end of three years when trees were removed per a landowner maintenance agreement, 86%, 71%, and 43% of the Russian olive trees were alive in the low-, medium-, and high-salinity soils, respectively. At this time, ‘NM2’ was the only hybrid poplar clone with similar survival to Russian olive in the high-salinity soils. Russian olive had greater Na, Cd, and Fe leaf concentrations than the hybrid poplar clones, but it also had the worst uptake of Ca and Mg of all genotypes. For hybrid poplar, the P. deltoides × P. nigra genomic group had the broadest clonal variability among all traits, with ‘Robusta’ and ‘DN182’ exhibiting great potential for establishment on high-salinity soils. ‘Robusta’ and ‘DN17’ are the same genotype but they came from different nursery sources (i.e., hence their different nomenclature), and they did not differ for height nor leaf phytoextraction. Populus deltoides × P. maximowiczii clones were not suitable for the soil conditions and silvicultural applications (e.g., tree shelters) of the current study, while P. nigra × P. maximowiczii clones exhibited the most stable performance across all years and salinity treatments. Both ‘NM2’ and ‘NM6’ had superior fourth-year survival and height, as well as average or above average phytoextraction of all elements tested.

Description: Manglietia patungensis (Magnoliaceae) exhibits radially symmetric flowers with perianth consisting of three separate sepaloid tepals in whorl 1 and six petaloid tepals in the inner two whorls, which shows an obvious difference from flowers of most Magnoliaceae species that contain three uniform petaloid tepals whorls, and make it an excellent model for understanding perianth morphology differentiation during early flower evolution. Here, two AGL6 orthologs, MapaAGL6-1 and MapaAGL6-2, were isolated from M. patungensis. Sequence alignment and phylogenetic analyses grouped both genes into the AGL6 lineage. MapaAGL6-1 is expressed only in the perianth whorls, while MapaAGL6-2 is strongly expressed in the perianth whorls but is lowly expressed in gynoecium. Furthermore, ectopic expression of MapaAGL6-1 results in strong complementation phenotypes in the Arabidopsis ap1-10 flower and production of normal floral organs in four floral whorls only with the petal number reduced in whorl 2, while ectopic expression of MapaAGL6-2 only results in petals partly rescued but failing to terminate carpelloid development in Arabidopsis ap1-10 mutant. In addition, the daughter lines generated from a cross between 35S::MapaAGL6-1 transgenic plants showing strong phenotypes and 35S::MapaAGL6-2 transgenic plants showing phenotypic changes produce normal flowers. Our results suggest that MapaAGL6-1 is a reasonable A-function gene controlling perianth identity in Magnoliaceae, which infers from its expression region and complementation phenotypes in Arabidopsis ap1 mutant, while MapaAGL6-2 is mainly involved in petaloid tepal development. Our data also provide a new clue to uncover the perianth development and early evolution in basal angiosperms.

Description: Whitebark pine, a foundation species at tree line in the Western U.S. and Canada, has declined due to native mountain pine beetle epidemics, wildfire, and white pine blister rust. These declines are concerning for the multitude of ecosystem and human benefits provided by this species. An understanding of the climatic correlates associated with spread is needed to successfully manage impacts from forest pathogens. Since 2000 mountain pine beetles have killed 75% of the mature cone-bearing trees in the Greater Yellowstone Ecosystem, and 40.9% of monitored trees have been infected with white pine blister rust. We identified models of white pine blister rust infection which indicated that an August and September interaction between relative humidity and temperature are better predictors of white pine blister rust infection in whitebark pine than location and site characteristics in the Greater Yellowstone Ecosystem. The climate conditions conducive to white pine blister rust occur throughout the ecosystem, but larger trees in relatively warm and humid conditions were more likely to be infected between 2000 and 2018. We mapped the infection probability over the past two decades to identify coarse-scale patterns of climate conditions associated with white pine blister rust infection in whitebark pine.

Description: Poplar (Populus × xiaohei T. S. Hwang et Liang) is an excellent model plant, with a known genome sequence, for studying woody plant developmental processes, such as seed germination. Here, we report the transcriptional profiling of poplar seeds at five germination stages using RNA-Seq technology. We focused on identifying biological pathway activity changes during seed germination and transcription factors that play important roles in different stages. Among the 16 significantly changing clusters obtained using the STEM method, transcription was significantly enriched in five different clusters, 8, 21, 25, 27, and 35. The oxidative phosphorylation-related genes were only enriched in cluster 9, and expression patterns decreased in 6 and 24 HAI, while ubiquitin-dependent protein catabolic processes were only enriched in cluster 16, and expression patterns increased in 6 HAI. A weighted mean method analysis determined that most primary metabolism-associated categories, such as major carbohydrate metabolism, glycolysis, oxidative pentose phosphate, tricarboxylic acid cycle, lipid metabolism, nucleotide metabolism, amino acid metabolism, and protein metabolism, were elevated between 6 and 48 h after imbibition (HAI). ATP synthesis and C1 metabolism had highly active expression patterns between 0.75 and 48 HAI. The photosynthesis category-associated genes that were identified appeared highly active at 144 HAI. The homogenization of transcription factors in each cluster revealed that the HAP2, C3H zinc finger family, and C2C2(Zn) GATA transcription factors were present in relatively high numbers in cluster 8, while HAP5, Zn-finger (CCHC), FHA, and E2F/DP transcription factor families, as well as SNF7, were present in high numbers in cluster 25. Thus, we identified a series of biological pathway activity changes that occur, and transcription factors that are active, during poplar seed germination. Moreover, this study provides an integrated view of transcriptional regulation that can reveal the molecular events occurring during seed germination.

Description: Anthocyanins play many roles in plants, including providing protection from biotic and abiotic stresses. Japanese apple rust (Gymnosporangium yamadae Miyabe ex G. Yamada) causes serious diseases in plants of the genus Malus and results in reduced fruit production and quality. However, few studies have been done to unravel the molecular mechanisms of anthocyanin formation in rust-infected apple leaves. To identify new regulatory genes in apple leaves that may be involved in regulating rust-induced anthocyanin biosynthesis, we measured anthocyanin content and sequenced the transcriptomes of rust-infected and uninfected tissues of Malus ‘Profusion’ leaves. Significant color changes and anthocyanin enrichment (especially cyanidin-3-galactoside chloride) occurred in infected tissues, whereas no significant color change and a low anthocyanin level were observed in uninfected tissue. We identified 10,045 differentially expressed genes (DEGs) in these two tissue types, including 6021 genes that were upregulated in the infected tissue and 4024 genes that were downregulated. We also identified five structural genes that are putative regulators of anthocyanin biosynthesis. In addition, 56 MYB genes, 36 bHLH genes, and one WD40 gene were identified among the obtained DEGs. According to the phylogeny of the amino acid sequences of transcription factors known to be involved in anthocyanin biosynthesis, one MYB gene (MYB114-like) and two bHLH genes (bHLH33 and bHLHA-like) may relate to anthocyanin biosynthesis in rust-infected apple leaves. These data will provide insights into the molecular mechanisms underlying anthocyanin accumulation upon rust infection.

Description: The method of forest biomass estimation based on a relationship between the volume and biomass has been applied conventionally for estimating stand above- and below-ground biomass (SABB, t ha−1) from mean growing stock volume (m3 ha−1). However, few studies have reported on the diagnosis of the volume-SABB equations fitted using field data. This paper addresses how to (i) check parameters of the volume-SABB equations, and (ii) reduce the bias while building these equations. In our analysis, all equations were applied based on the measurements of plots (biomass or volume per hectare) rather than individual trees. The volume-SABB equation is re-expressed by two Parametric Equations (PEs) for separating regressions. Stem biomass is an intermediate variable (parametric variable) in the PEs, of which one is established by regressing the relationship between stem biomass and volume, and the other is created by regressing the allometric relationship of stem biomass and SABB. A graphical analysis of the PEs proposes a concept of “restricted zone,” which helps to diagnose parameters of the volume-SABB equations in regression analyses of field data. The sampling simulations were performed using pseudo data (artificially generated in order to test a model) for the model test. Both analyses of the regression and simulation demonstrate that the wood density impacts the parameters more than the allometric relationship does. This paper presents an applicable method for testing the field data using reasonable wood densities, restricting the error in field data processing based on limited field plots, and achieving a better understanding of the uncertainty in building those equations.

Description: The dove tree (Davidia involucrata Baill.), a tertiary relic species, is adapted to cool climates. With the progression of global warming, high-temperature stress has become the primary environmental factor restricting geographic distribution, ex situ conservation, and landscape application for D. involucrata resources. However, the detailed molecular events underlying D. involucrata responses to heat stress are poorly understood. Here, we conducted RNA-Seq-based gene expression profiling in D. involucrata seedlings during the time course of a 42 °C heat treatment (0, 1, 6, and 12 h). After de novo assembly, we obtained 138,923 unigenes, of which 69,743 were annotated in public databases. Furthermore, 19,532, 20,497 and 27,716 differentially expressed genes (DEGs) were identified after 1 h (HS1), 6 h (HS6), and 12 h (HS12) of heat treatment in comparison to 0 h (HS0), respectively. Based on a KEGG enrichment analysis, the two pathways “protein processing in endoplasmic reticulum” and “plant hormone signal transduction” are hypothesized to play vital roles during heat response in D. involucrata, and their potential interactions during heat stress are also discussed. In addition, 32 genes encoding putative heat shock transcription factors (Hsfs) were found to be associated with the response of D. involucrata to heat stress. Finally, the expression patterns of eight heat-responsive genes derived from qRT-PCR were in agreement with their transcript level alterations, as determined by a transcriptome analysis. Taken together, our transcriptomic data provide the first comprehensive transcriptional profile affected by heat stress in D. involucrata, which will facilitate further studies on the improvement of heat tolerance in this rare and endangered species.

Description: Fusarium circinatum is the causal agent of pine pitch canker (PPC), one of the most devastating forest diseases worldwide. This fungus causes severe damping-off in pine seedlings and growth reduction, wilting and the development of cankers in pine forests and plantations. A draft of the complete genome sequence of this phytopathogen was recently made available. This information was used to annotate in silico the gene Fcrho1 as a putative homolog of Rho1 GTPase genes. In this study, we generated Fcrho1 deletion mutants in two F. circinatum wildtype strains isolated from damaged trees in northern Spain. For that, we used a modified version of the OSCAR methodology, an approach not previously used in F. circinatum that allows the generation of deletion constructs in a single cloning step. The conidiation and spore germination of the resulting deletion mutants were not affected, neither the hyphal morphology. However, the mutant strains showed significantly reduced growth in vitro and more foamy macroscopic hyphal morphology than their corresponding ectopic and wildtype strains. Finally, an in vivo virulence assay showed that the reduced in vitro growth rate characteristic to the deletion mutants does not impact their pathogenicity.

Description: Operational studies are necessary to support production and management decisions of forest industries. A time study (TS) approach is widely used in timber harvesting operations to understand the performance of individual harvesting machines as well as the entire system. However, several limitations of the TS approach include the use of generalized utilization rates, incapability of capturing interactions among equipment, and model extrapolation in sensitivity analysis. In this study, we demonstrated the use of discrete event simulation (DES) techniques in modeling a ground-based timber harvesting system, and compared the DES results with those of the TS model developed with the same observed data. Although both TS and DES models provided similar estimation results for individual machine cycle times and productivities, the estimated machine utilization rates were somewhat different due to the difference in synthesizing machine processes in each approach. Our sensitivity analysis and model expansion to simulate a hypothetical harvesting system suggest that the DES approach may become an appropriate method for analyzing complex systems especially where interactions among different machine processes are unknown.

Description: Monitoring vegetation characteristics and ground cover is crucial to determine appropriate management techniques in western juniper (Juniperus occidentalis Hook.) ecosystems. Remote-sensing techniques have been used to study vegetation cover; yet, few studies have applied these techniques using unmanned aerial vehicles (UAV), specifically in areas of juniper woodlands. We used ground-based data in conjunction with low-altitude UAV imagery to assess vegetation and ground cover characteristics in a paired watershed study located in central Oregon, USA. The study was comprised of a treated watershed (most juniper removed) and an untreated watershed. Research objectives were to: (1) evaluate the density and canopy cover of western juniper in a treated (juniper removed) and an untreated watershed; and, (2) assess the effectiveness of using low altitude UAV-based imagery to measure juniper-sapling population density and canopy cover. Ground- based measurements were used to assess vegetation features in each watershed and as a means to verify analysis from aerial imagery. Visual imagery (red, green, and blue wavelengths) and multispectral imagery (red, green, blue, near-infrared, and red-edge wavelengths) were captured using a quadcopter-style UAV. Canopy cover in the untreated watershed was estimated using two different methods: vegetation indices and support vector machine classification. Supervised classification was used to assess juniper sapling density and vegetation cover in the treated watershed. Results showed that vegetation indices that incorporated near-infrared reflectance values estimated canopy cover within 0.7% to 4.1% of ground-based calculations. Canopy cover estimates at the untreated watershed using supervised classification were within 0.9% to 2.3% of ground-based results. Supervised classification applied to fall imagery using multispectral bands provided the best estimates of juniper sapling density compared to imagery taken in the summer or to using visual imagery. Study results suggest that low-altitude multispectral imagery obtained using small UAV can be effectively used to assess western juniper density and canopy cover.

Description: Research Highlights: this study developed additive biomass equations respectively from nonlinear regression (NLR) on original data and linear regression (LR) on a log-transformed scale by nonlinear seemingly unrelated regression (NSUR). To choose appropriate regression form, the error structures (additive vs. multiplicative) of compatible biomass equations were determined on the use of the multivariate likelihood function which extended the method of likelihood analysis to the general occasion of a contemporaneously correlated set of equations. Background and Objectives: both NLR and LR could yield the expected predictions for allometric scaling relationship. In recent studies, there are vigorous debates on which regression (NLR or LR) should apply. The main aim of this paper is to analyze the error structure of a compatible system of biomass equations to choose more appropriate regression. Materials and Methods: based on biomass data of 270 trees for three tree species, additive biomass equations were developed respectively for NLR and LR by NSUR. Multivariate likelihood functions were computed to determine the error structure based on the multivariate probability density function. The anti-log correction factor which kept the additive property was obtained separately using the arithmetic and weighted average of basic correction factors from each equation to assess two model specifications on the comparably original scale. Results: the assumption of additive error structure was well favored for an additive system of three species based on the joint likelihood function. However, the error structure of each component equation calculated from the conditional likelihood function for compatible equations might be different. The performance of additive equations corrected by a weighted average of basic correction factor from each component equation performed better than that of the arithmetic average and held good property of compatibility after corrected. Conclusions: NLR provided a better fit for additive biomass equations of three tree species. Additive equations which confirmed the responding assumption of error structure performed better. The joint likelihood function on the use of the multivariate likelihood function could be used to analyze the error structure of the additive system which was a result of a tradeoff for each component equation. Based on the average of correction factors from each component equation to correct the bias of additive equations was feasible for the hold of additive property, which might lead to a poor correction effect for some component equation.

Description: Visual sensitivity assessments identify the location of the high-sensitivity areas in terms of visual change. Studying the visual sensitivity of plantation forest landscapes and their seasonal changes can help resolve increasingly frequent conflicts between tourism and forest management activities, in the context of the multi-functional management of plantation forests. In this study, we used the geographic information system (GIS) and multi-criteria evaluation (MCE) methods combined with the analytic hierarchy process (AHP) to perform a visual sensitivity evaluation. Nine map-based criteria were selected, and the visual sensitivity of summer and autumn values were calculated, using data from sources including inventory data for forest management planning and design, digital elevation model (DEM), and aerial photographs. Vegetation uniformity (VU) and color diversity (CD) indices were constructed using three patch-level-based landscape indices, including area (AREA), fractal dimension index (FRAC), and proximity (PROX), to visualize the summer and autumn vegetation characteristics of a plantation forest landscape. We conducted a case study on the Saihanba Mechanical Forest Plantation, China’s largest forest plantation. The results were evaluated by experts, confirming the method to be reliable. This study provides an accurate, objective, and visualized evaluation method for the visual sensitivity of plantations for forest management units at the landscape scale. In analyzing the visual sensitivity of plantation forest landscapes, appropriate criteria (e.g., uniformity or diversity should be selected based on forest vegetation characteristics). When identifying high-sensitivity regions, it is necessary to simultaneously analyze areas with high visual sensitivity in different seasons and then superimpose the results.

Description: Research Highlights: Two genets of Armillaria altimontana Brazee, B. Ortiz, Banik, and D.L. Lindner and five genets of Armillaria solidipes Peck (as A. ostoyae [Romagnesi] Herink) were identified and spatially mapped within a 16-year-old western white pine (Pinus monticola Doug.) plantation, which demonstrated distinct spatial distribution and interspecific associations. Background and Objectives: A. solidipes and A. altimontana frequently co-occur within inland western regions of the contiguous USA. While A. solidipes is well-known as a virulent primary pathogen that causes root disease on diverse conifers, little has been documented on the impact of A. altimontana or its interaction with A. solidipes on growth, survival, and the Armillaria root disease of conifers. Materials and Methods: In 1971, a provenance planting of P. monticola spanning 0.8 ha was established at the Priest River Experimental Forest in northern Idaho, USA. In 1987, 2076 living or recently dead trees were measured and surveyed for Armillaria spp. to describe the demography and to assess the potential influences of Armillaria spp. on growth, survival, and the Armillaria root disease among the study trees. Results: Among the study trees, 54.9% were associated with Armillaria spp. The genets of A. altimontana and A. solidipes comprised 82.7% and 17.3% of the sampled isolates (n = 1221) from the study plot, respectively. The mapped distributions showed a wide, often noncontiguous, spatial span of individual Armillaria genets. Furthermore, A. solidipes was found to be uncommon in areas dominated by A. altimontana. The trees colonized by A. solidipes were associated with a lower tree growth/survival and a substantially higher incidence of root disease than trees colonized only by A. altimontana or trees with no colonization by Armillaria spp. Conclusions: The results demonstrate that A. altimontana was not harmful to P. monticola within the northern Idaho planting. In addition, the on-site, species-distribution patterns suggest that A. altimontana acts as a long-term, in situ biological control of A. solidipes. The interactions between these two Armillaria species appear critical to understanding the Armillaria root disease in this region.

Description: The number of mixed cropland—apple orchard system has gradually increased in the Changwu Tableland region of the Loess Plateau, China. However, the soil water content (SWC) is not sufficient to maintain the sustainable development of apple trees in this agroforestry system. It is unclear whether the growing fruit trees would compete with crops for soil water. To systematically analyze the temporal and spatial distribution of soil moisture and to understand the effect of orchard hydrology in that cropland, the SWC was measured at different depths at different locations on cropland and in an apple orchard. The results show that: (1) The SWC of each soil layer in the cropland (0–20, 20–60, 60–100, 100–200, 200–300 cm) is higher than that of the orchard. The soil moisture changes dramatically in the 0–200 cm soil layer. (2) As the soil moisture monitoring distance from the apple orchard increases, the SWC gradually increases, the loss of soil water storage gradually decreases, and the drying effect gradually disappears. This is related to the different distribution ranges of the roots of apple trees and crops. Therefore, the government should control the proportion of the orchard and cropland, and then adjust the planting period of the orchard in the appropriate range to keep the green use of water in the region.

Description: Continuous research into the availability of phosphorus (P) in forest soil is critical for the sustainable management of forest ecosystems. In this study, we used sequential chemical extraction and 31P-nuclear magnetic resonance spectroscopy (31P-NMR) to evaluate the form and distribution of inorganic P (Pi) and organic P (Po) in Casuarina forest soils of a subtropical coastal sand dune in Houlong, Taiwan. The soil samples were collected from humic (+2–0 cm) and mineral layers (mineral-I: 0–10, mineral-II: 10–20 cm) at two topographic locations (upland and lowland) with different elevations. Sequential chemical extraction revealed that the NaOH-Po fraction, as moderately recalcitrant P, was the dominant form in humic and mineral-I layers in both upland and lowland soils, whereas the cHCl-Pi fraction was the dominant form in the mineral-II layer. The resistant P content, including NaOH-Pi, HCl-Pi, cHCl-Pi, and cHCl-Po fractions, was higher in the upland than in the lowland. However, the labile P content, NaHCO3-Po, showed the opposite pattern. The content of resistant Pi (NaOH-Pi, HCl-Pi, and cHCl-Pi) increased significantly with depth, but that of labile Pi (resin-Pi and NaHCO3-Pi) and recalcitrant Po (NaHCO3-Po, NaOH-Po, and cHCl-Po) decreased significantly with depth at both locations. 31P-NMR spectroscopy revealed inorganic orthophosphate and monoesters-P as the major forms in this area. The proportions of Pi and Po evaluated by sequential chemical extraction and 31P-NMR spectroscopy were basically consistent. The results indicate that the soils were in weathered conditions. Furthermore, the P distribution and forms in this coastal sand dune landscape significantly differed between the upland and lowland because of the variation in elevation and eolian aggradation effects.

Description: The height of tree stumps following mechanized forest operations can be influenced by machine-, tree-, terrain-, and operator-related characteristics. High stumps may pose different economic and technical disadvantages. Aside from a reduction in product recovery (often associated with sawlog potential), leaving high stumps can complicate future entries if smaller equipment with low ground clearance is used, particularly in the case where new machine operating trails are required. The objective of this exploratory study was to examine if correlations existed between the height of tree stumps following mechanized harvesting and the shape of the above-ground root collar, stump diameter, and distance to the machine operating trail. In total, 202 sample stumps of Norway spruce (Picea abies (L.) Karst.) and the surrounding terrain were scanned with a terrestrial laser scanner. The collected data was processed into a 3D-model and then analyzed. Stump height was compared with different characteristics such as stump diameter at the cut surface, distance to the machine operating trail, number of visible root flares per stump, and the root collar. The number of root flares per stump had a positive influence on stump diameter and height, showing a general trend of increasing diameter and height with the increasing number of root flares. Root angles also had an influence on the stump diameter. The diameter of a stump and the shape of the root collar at the cut surface together had a significant effect on stump height and the model reported explained half of the variation of stump heights. Taken together, these findings suggest that other factors than the ones studied can also contribute in influencing stump height during mechanized harvesting operations. Further investigations, including pre- and post-harvest scans of trees selected for removal, are warranted.

Description: Working time studies, work productivity, and cost assessments of forest logging are of interest to forest managers and planners. These aspects are particularly important in salvage logging, because of difficulties due to irregularly positioned fallen trees in forest areas, and due to particular aspects related to the absence of work planning. In this research, system productivity and the cost of salvage logging are analyzed for two mountain forests managed with close-to nature-silviculture: the Hyrcanian forest, using extraction by a skidder, and the Camaldoli forest, using extraction by tractors. The system productivity of salvage logging by skidders and tractors was calculated as 1.54 and 0.81 m3·h−1, respectively. In contrast to common logging, system productivity was about 6- to 15-fold lower in salvage logging. The effective cost consumptions for the skidder and tractor were calculated as $72.57 and $118.62 USD·m−3, respectively. For both yards, winching time increased due to increasing winching distance and winching load volume. The same result was determined for skidding time in relation to load volume and distance. The possible cost decreases for the skidder and tractor were calculated as 2.6% and 4.3%, respectively. The results revealed that operational costs for extracting wind-fallen trees are higher than for traditional standing-trees extraction for both situations studied. In both cases, the harvesting costs were higher than the timber price by 10% to 30%. Therefore, extraction of wind-fallen trees has no economic justification in these forests.

Description: Research Highlights: Plant cover drives the activity of the microbial decomposer community and affects carbon (C) sequestration in the soil. Despite the relationship between microbial activity and C sequestration in the soil, potential inhibition of soil microbial activity by plant cover has received little attention to date. Background and Objectives: Differences in soil microbial activity between two paired stands on soil at a very early stage of formation and a common story until afforestation, can be traced back to the plant cover. We hypothesized that in a black locust (Robinia pseudoacacia L.) stand the high-quality leaf litter of the tree, and that of the blackberry (Rubus fruticosus L.) understory had an inhibitory effect on soil microbial community resulting in lower mineralization of soil organic matter compared to the paired black pine (Pinus nigra Arn.) stand. Materials and Methods: We estimated potential mineralization rates (MR), microbial (MB), and active fungal biomass (AFB) of newly-shed litter, forest floor, and mineral soil. We tested the effects of litters’ water extracts on soil MR, MB, AFB and its catabolic response profile (CRP). Results: Newly-shed litter of black locust had higher MR than that of blackberry and black pine; MR, MB, and AFB were higher in forest floor and in mineral soil under black pine than under black locust. Water extracts of black locust and blackberry litter had a negative effect on the amount, activity of microorganisms, and CRP. Conclusions: The results demonstrate the potential for black locust and blackberry litter to have a marked inhibitory effect on decomposer microorganisms that, in turn, reduce organic matter mineralization with possible consequences at the ecosystem level, by increasing C sequestration in mineral soil.

Description: Due to its advantages of good heat-resistance, environmental-friendliness, and low cost, bamboo grid packing (BGP) has become a promising new type of cooling packing. It is being increasingly used in Chinese industrial cooling towers to replace cooling packings made of polyvinyl chloride, cement, and glass fiber reinforced plastic. However, mechanical properties and fungal resistance are a concern for all bamboo applications. In this study, the modulus of rupture (MOR), modulus of elasticity (MOE), density, crystallinity, and environment scanning electron microscope (ESEM) properties were compared between fresh BGPs and those that had been in service for nine years in the cooling towers. The results showed that the MOR, MOE, density, crystallinity, and the crystal size of the used BGPs decreased to some extent, but still met the requirements for normal use in a cooling tower. The ESEM observation showed that the used BGPs were not infected by fungi. The decrease in mechanical properties could be caused by the decrease of density, crystallinity, and the decomposition of the chemical components of bamboo, but not by fungal infection.

Description: Wood density profiles reveal a tree’s life strategy and growth. Density profiles are, however, rarely defined in terms of tissue fractions for wood of tropical angiosperm trees. Here, we aim at linking these fractions to corresponding density profiles of tropical trees from the Congo Basin. Cores of 8 tree species were scanned with X-ray Computed Tomography to calculate density profiles. Then, cores were sanded and the outermost 3 cm were used to semi-automatically measure vessel lumen, parenchyma and fibre fractions using the Weka segmentation tool in ImageJ. Fibre wall and lumen widths were measured using a newly developed semi-automated method. An assessment of density variation in function of growth ring boundary detection is done. A mixed regression model estimated the relative contribution of each trait to the density, with a species effect on slope and intercept of the regression. Position-dependent correlations were made between the fractions and the corresponding wood density profile. On average, density profile variation mostly reflects variations in fibre lumen and wall fractions, but these are species- and position-dependent: on some positions, parenchyma and vessels have a more pronounced effect on density. The model linking density to traits explains 92% of the variation, with 65% of the density profile variation attributed to the three measured traits. The remaining 27% is explained by species as a random effect. There is a clear variation between trees and within trees that have implications for interpreting density profiles in angiosperm trees: the exact driving anatomical fraction behind every density value will depend on the position within the core. The underlying function of density will thus vary accordingly.

Description: Wall-to-wall tree-lists information (lists of species and diameter for every tree) at a regional scale is required for managers to assess forest sustainability and design effective forest management strategies. Currently, the k-nearest neighbors (kNN) method and the Weibull diameter distribution function have been widely used for estimating tree lists. However, the kNN method usually relies on a large number of field inventory plots to impute tree lists, whereas the Weibull function relies on strong correlations between stand attributes and diameter distribution across large regions. In this study, we developed a framework to estimate wall-to-wall tree lists over large areas based on a limited number of forest inventory plots. This framework integrates the ability of extrapolating diameter distribution from Weibull and kNN imputation of wall-to-wall forest stand attributes from Moderate Resolution Imaging Spectroradiometer (MODIS). We estimated tree lists using this framework in Chinese boreal forests (Great Xing’an Mountains) and evaluated the accuracy of this framework. The results showed that the passing rate of the Kolmogorov–Smirnov (KS) test for Weibull diameter distribution by species was from 52% to 88.16%, which means that Weibull distribution could describe the diameter distribution by species well. The imputed stand attributes (diameter at breast height (DBH), height, and age) from the kNN method showed comparable accuracy with the previous studies for all species. There was no significant difference in the tree density between the estimated and observed tree-lists. Results suggest that this framework is well-suited to estimating the tree-lists in a large area. Our results were also ecologically realistic, capturing dominant ecological patterns and processes.

Description: Individual tree crown (ITC) segmentation is an approach to isolate individual tree from the background vegetation and delineate precisely the crown boundaries for forest management and inventory purposes. ITC detection and delineation have been commonly generated from canopy height model (CHM) derived from light detection and ranging (LiDAR) data. Existing ITC segmentation methods, however, are limited in their efficiency for characterizing closed canopies, especially in tropical forests, due to the overlapping structure and irregular shape of tree crowns. Furthermore, the potential of 3-dimensional (3D) LiDAR data is not fully realized by existing CHM-based methods. Thus, the aim of this study was to develop an efficient framework for ITC segmentation in tropical forests using LiDAR-derived CHM and 3D point cloud data in order to accurately estimate tree attributes such as the tree height, mean crown width and aboveground biomass (AGB). The proposed framework entails five major steps: (1) automatically identifying dominant tree crowns by implementing semi-variogram statistics and morphological analysis; (2) generating initial tree segments using a watershed algorithm based on mathematical morphology; (3) identifying “problematic” segments based on predetermined set of rules; (4) tuning the problematic segments using a modified distance-based algorithm (DBA); and (5) segmenting and counting the number of individual trees based on the 3D LiDAR point clouds within each of the identified segment. This approach was developed in a way such that the 3D LiDAR points were only examined on problematic segments identified for further evaluations. 209 reference trees with diameter at breast height (DBH) ≥ 10 cm were selected in the field in two study areas in order to validate ITC detection and delineation results of the proposed framework. We computed tree crown metrics (e.g., maximum crown height and mean crown width) to estimate aboveground biomass (AGB) at tree level using previously published allometric equations. Accuracy assessment was performed to calculate percentage of correctly detected trees, omission and commission errors. Our method correctly identified individual tree crowns with detection accuracy exceeding 80 percent at both forest sites. Also, our results showed high agreement (R2 〉 0.64) in terms of AGB estimates using 3D LiDAR metrics and variables measured in the field, for both sites. The findings from our study demonstrate the efficacy of the proposed framework in delineating tree crowns, even in high canopy density areas such as tropical rainforests, where, usually the traditional algorithms are limited in their performances. Moreover, the high tree delineation accuracy in the two study areas emphasizes the potential robustness and transferability of our approach to other densely forested areas across the globe.

Description: Climate change is likely to lead to an increased frequency of droughts and floods, both of which are implicated in large-scale carbon allocation and tree mortality worldwide. Non-structural carbohydrates (NSCs) play an important role in tree survival under stress, but how NSC allocation changes in response to drought or waterlogging is still unclear. We measured soluble sugars (SS) and starch in leaves, twigs, stems and roots of Robinia pseudoacacia L. seedlings that had been subjected to a gradient in soil water availability from extreme drought to waterlogged conditions for a period of 30 days. Starch concentrations decreased and SS concentrations increased in tissues of R. pseudoacacia seedlings, such that the ratio of SS to starch showed a progressive increase under both drought and waterlogging stress. The strength of the response is asymmetric, with the largest increase occurring under extreme drought. While the increase in SS concentration in response to extreme drought is the largest in roots, the increase in the ratio of SS to starch is the largest in leaves. Individual components of SS showed different responses to drought and waterlogging across tissues: glucose concentrations increased significantly with drought in all tissues but showed little response to waterlogging in twigs and stems; sucrose and fructose concentrations showed marked increases in leaves and roots in response to drought but a greater response to drought and waterlogging in stems and twigs. These changes are broadly compatible with the roles of individual SS under conditions of water stress. While it is important to consider the role of NSC in buffering trees against mortality under stress, modelling this behaviour is unlikely to be successful unless it accounts for different responses within organs and the type of stress involved.

Description: The Loess Plateau is an important region for vegetation restoration in China; however, changes in soil organic carbon (SOC), soil nutrients, and stoichiometry after restoration in this vulnerable ecoregion are not well understood. Typical restoration types, including orchardland, grassland, shrubland, and forestland, were chosen to examine changes in the stocks and stoichiometry of SOC, soil total nitrogen (TN), and soil total phosphorus (TP) at different soil depths and recovery times. Results showed that SOC stocks first increased and then stabilized in orchardland, grassland, and shrubland at 0–30 cm depths, while in forestland, SOC stocks gradually increased. Soil TN stocks first increased and then decreased in orchardland, shrubland, and forestland with restoration age at 0–30 cm depths, while soil TP stocks showed little variation between restoration types; at the same time, the overall C:N, C:P, and N:P ratios increased with restoration age. In the later stages of restoration, the stocks of SOC and soil TN at 0–30 cm soil depths were still lower than those in natural grassland and natural forest. Additionally, the SOC, soil TN, and soil TP stocks and the C:N, C:P, and N:P ratios decreased with soil depth. The forestland had the highest rate of change in SOC and soil TN stocks, at 0–10 cm soil depth. These results indicate a complex response of SOC, soil TN, and soil TP stocks and stoichiometry to vegetation restoration, which could have important implications for understanding C, N, and P changes and nutrient limitations after vegetation restoration.

Description: Soil enzymes and microbial communities are key factors in forest soil ecosystem functions and are affected by stand age. In this study, we studied soil enzyme activities, composition and diversity of bacterial and fungal communities and relevant physicochemical properties at 0–10 cm depth (D1), 10–20 cm depth (D2) and 20–30 cm depth (D3) soil layers in 3-(3a), 6-(6a), 12-(12a), 18-(18a), 25-(25a), 32-(32a) and 49-year-old (49a) Chinese fir plantations to further reveal the effects of stand age on soil biotic properties. Spectrophotometry and high-throughput sequencing was used to assess the soil enzyme activity and microbial community composition and diversity of Chinese fir plantation of different stand ages, respectively. We found that soil catalase activity increased as the stand age of Chinese fir plantations increased, whereas the activities of urease, sucrase and β-glucosidase in 12a, 18a and 25a were lower than those in 6a, 32a and 49a. Shannon and Chao1 indices of bacterial and fungal communities first decreased gradually from 6a to 18a or 25a and then increased gradually from 25a to 49a. Interestingly, the sucrase and β-glucosidase activities and the Shannon and Chao1 indices in 3a were all lower than 6a. We found that the relative abundance of dominant microbial phyla differed among stand ages and soil depths. The proportion of Acidobacteria first increased and then decreased from low forest age to high forest age, and its relative abundance in 12a, 18a and 25a were higher than 3a, 32a and 49a, but the proportion of Proteobacteria was opposite. The proportion of Ascomycota first decreased and then increased from 6a to 49a, and its relative abundance in 12a, 18a and 25a was lower than 3a, 6a, 32a and 49a. Our results indicate that soil enzyme activities and the richness and diversity of the microbial community are limited in the middle stand age (from 12a to 25a), which is important for developing forest management strategies to mitigate the impacts of degradation of soil biological activities.

Description: The contributions of heterotrophic respiration (RH) to total soil respiration (RS) for the non-growing season, growing season, and annual period are 84.8%, 60.7%, and 63.3%, respectively.Few studies have partitioned RS into its rhizospheric (RR) and heterotrophic components throughout the year in northern forest ecosystems. Our objectives were to quantify the contributions of non-growing season and heterotrophic respiration. We conducted a trenching experiment to quantify RR and RH in a temperate deciduous forest in Northeast China over two years using chamber methods. Temperature sensitivities (Q10) for RS and for RH were both much higher in the non-growing season (November to April) than those in the growing season. The Q10 for RS was higher than Q10 for RH in both seasons, indicating a higher temperature sensitivity of roots versus microorganisms. Mean non-growing season RS, RH, and RR for the two years were 94, 79 and 14 g carbon (C) m−2, respectively, which contributed 10.8%, 14.5%, and 4.5% to the corresponding annual fluxes (869, 547 and 321 g C m−2 year−1, respectively). The contributions of RH to RS for the non-growing season, growing season, and annual period were 84.8%, 60.7%, and 63.3%, respectively. Using the same contribution of non-growing season RS to annual RS, to scale growing season measurements, to the annual scale would introduce significant biases on annual RH (−34 g C m−2 yr−1 or −6%) and RR (16 g C m−2 yr−1 or 5%).We concluded that it was important to take non-growing season measurements in terms of accurately partitioning RS components in northern forests.

Description: Comprehensive evaluation of forest state is the precondition and critical step for forest management. To solve the problem that the radar plot and unit circle only focus on the value of each the evaluation index, this paper proposes a novel method for comprehensively and simultaneously evaluating the functionality and inhomogeneity of forest state based on the modified unit circle method. We evaluated the forest state of the Quercus aliena BL. var. acuteserrata Maxim. ex Wenz. broad-leaved mixed forest in the Xiaolong Mountains Forest Area of Gansu Province and the Pinus koraiensis Sieb. et Zucc. broad-leaved mixed forest in Jilin Province in China. According to the principle of comprehensive, scientific and operability, 10 evaluation indices on forest structure and vitality were selected to construct the evaluation indicator system. Each index was normalized based on the assignment method and ensured to be strictly positive based on reciprocal transformation method. The areas and arc length of the closed graph, formed by connecting every two adjacent indicators, in the radar plot and unit circle were extracted. Based on the isoperimetric theorem (isoperimetric inequality), a comprehensive evaluation model was constructed. Compared with radar chart and unit circle method, each index in the newly proposed unit circle method is represented by an independent sector region, reflecting the contribution of the index to the overall evaluation result. Each index has the same relative importance weight, contributing to the estimation the relative sizes of each aspect of forest state. The unique area and arc length of the closed graph help summarize the overall performance with a global score. The expression effect of improved unit circle has been enhanced, and as an English proverb put it, “A picture is worth a thousand words.” The new proposed method simultaneously evaluates the functionality and inhomogeneity of the forest state and it is a powerful tool for the diagnosis of forest state problems and the decision-making of forest management.