ALBERT

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉An assessment of the influence and significance of different topographic and morphometric parameters of glacier catchments such as size, average slope, median elevation, and aspect upon the spatio-temporally changing characteristics of glaciers belonging to different size classes in Tista river basin of Sikkim Himalaya has been performed. The reference years of 2000 and 2018 were considered and different morphometric techniques showed recessive nature and shape change of the observed main glaciers along with some of the tributary glaciers. Pearson’s correlation coefficient and Student’s 〈em〉t〈/em〉 test were applied to measure the correlation and statistical significance of used parameters to understand the varying morphometric and topographic conditions of the glaciers. LANDSAT TM (thematic mapper), LANDSAT 7 ETM + (enhanced thematic mapper), and LANDSAT 8 (OLI) (operational land imager) imageries and shuttle radar topographic mission (SRTM) digital elevation model (DEM) and different automated and semi-automated techniques indicated overall loss of 19.78% over the years 2000 to 2018 of the glaciated area investigated and such decrease in respective years of reference was 2.06% for the year 2000 and 1.65% for the year 2018, respectively, of the total geographic area of Sikkim. TanDem-X DEM (90 m) was useful in inferring glacier surface elevation change.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉One of the most crucial design steps to guarantee the long life and the sustainability of artificial recharge of groundwater projects is to find the best locations. The present study focuses on identifying potential zones of groundwater artificial recharge in Shabestar region, northwest of Iran. For this purpose, random forest (RF) model, a learning method based on ensemble decision trees, was proposed for locating groundwater artificial recharge. Important factors, including slope and slope aspect, soil texture, erosion, land use, groundwater quality, permeability and geological lithology were integrated in a geographic information science (GIS). According to RF model, permeability and unsaturated zone thickness were identified as the most effective parameters for locating groundwater artificial recharge sites. Based on the proposed model, it was found that 14% of the region is located in suitable site for groundwater artificial recharge projects. The accuracy of the model was evaluated with receiver-operating characteristic (ROC) curve and the mean squared error (MSE). Low MSE and ROC curve of the model with the highest area under curve equal to 0.947, indicated high accuracy of random forest in locating groundwater artificial recharge.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Loktak Lake is the largest natural freshwater lake of Northeastern India, characteristic by floating islands, known as 〈em〉Phumdis〈/em〉. The lake supports rich biodiversity including some globally threatened species, hence declared as a Ramsar Site. The lake facilitates hydropower, irrigation and provides a livelihood to the local villagers in the form of fishing and agriculture. Loktak Lake is deteriorating in recent years due to rapid urbanization, increased settlement and other anthropogenic activities. A study was, therefore, undertaken to assess the water quality and its trends for this important lake. The water quality of Loktak Lake was assessed in monthly intervals for a period of 3 years, using multiple water quality indices. Temporal distribution functions of the individual water quality parameters and indices were developed to assess the water quality trend of the lake. Overall water quality of this lake was found to be good throughout the year with slight deterioration during winter. However, the water of this lake was a little turbid and contained significant organic load.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Shrub encroachment has profound influences on regional carbon cycling. However, few studies have examined the changes in soil organic carbon (SOC) components at the molecular level along a climate gradient. In this study, we aimed to investigate the effects of biotic and abiotic factors on the patterns of SOC components in the shrub patches and the grassy matrix.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We analyzed the distribution and controlling factors of SOC components (including free lipids, bound lipids, and lignin-derived phenols) in the topsoil of shrub-encroached grasslands along natural climate gradients in Inner Mongolia, China.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉 We found that the concentrations of bound lipids and lignin-derived phenols were significantly higher and the vanillic acid to vanillin ratio ((Ad/Al)〈sub〉v〈/sub〉) was significantly lower in the shrub patches than in the grassy matrix (〈em〉p〈/em〉 〈 0.05). After excluding variables exhibiting collinearity, redundancy analysis showed that shrub patch cover and soil pH were the most important variables that influenced SOC composition in the shrub patches, while herb characteristics and shrub density were the most important in the grassy matrix. Structural equation modeling showed that shrub characteristics at the plot scale greatly contributed to the variance in all components in the grassy matrix, whereas soil properties were more important in the shrub patches.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Our results highlight that although the topsoil carbon content did not change, shrub encroachment altered the SOC components and their drivers in the Inner Mongolian grasslands.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉While the coupled effects of root exudates and microbial feedbacks on soil processes are well-recognized, we still lack an understanding of differences in root exudate fluxes and the associated ecological consequences among tree growth forms.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Two deciduous tree species (i.e., 〈em〉Cercidiphyllum japonicum〈/em〉 and 〈em〉Larix kaempferi〈/em〉) and two evergreen tree species (i.e., 〈em〉Pinus armandi〈/em〉 and 〈em〉Pinus tabulaeformis〈/em〉) were selected to perform an in-situ collection of root exudates during the growing season in 2016. The net N mineralization rates and associated microbial enzyme activities were measured in rhizosphere and bulk soils to evaluate rhizosphere effects. Moreover, we compiled the dataset related to root exudation and their associated biological traits and the soil chemical properties for 21 tree species from temperate forests.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The root exudation rates and the annual root exudate carbon (C) fluxes of two deciduous tree species were significantly higher than those of the two evergreen tree species. Correspondingly, the rhizosphere effects of deciduous tree species on the microbial biomass, enzyme activity and net N mineralization rate were approximately 1.9, 1.6 and 2.4 times greater than those of the evergreen tree species, respectively. Rhizosphere effects were positively correlated with the root exudation rate. The compiled dataset also suggest that deciduous tree species tend to have higher exudation rates than evergreen tree species in temperate forests.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Collectively, these results suggest that the two tree growth forms exhibit different patterns in root exudate inputs and associated rhizosphere microbial processes. Generally, deciduous tree species tend to exude more C into the soil and consequently induce greater microbial feedback on soil N transformations during the growing season in temperate regions, implying that deciduous tree species induced a greater effect on the C and nutrient cycling in rhizosphere soil than evergreen tree species.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Culture media compositions and bioprocess conditions were studied to improve the production of cell biomass and indolic phytohormones by 〈em〉Herbaspirillum seropedicae〈/em〉 BR11471, a plant growth promoting bacterium, and different inoculant formulations were also produced and tested for their stability and shelf life.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Response surface methodology (RSM) based on central composite rotation designs (CCRD) was used to find bioprocess variables that lead to an increase in bacterial biomass and yield of indolic compounds. The major components of DYGS medium were optimized in small-scale shaken cultivations, in two sets of CCRD. High performance liquid chromatography was used to determine nutrient consumption and to correlate it with cell biomass production, and the Salkowski method was used to quantify indoles. Hydrolytic activity in the formulations was quantified with the fluorescein diacetate assay.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Glycerol (5.5 g L〈sup〉−1〈/sup〉) and yeast extract (2.8 g L〈sup〉−1〈/sup〉), as the main carbon and nitrogen sources, respectively, increased biomass production by 87.5% when compared to original DYGS medium, reaching 3.0 g L〈sup〉−1〈/sup〉 of dry cell weight (DCW). In a 2.0 L bioreactor, the optimized medium was used to enhance process conditions for DCW and indole-3-acetic acid (IAA). Biomass production reached 3.4 g L〈sup〉−1〈/sup〉 and was restrained at highest air flow levels. The conditions of 34-36 °C, 150 rpm and 4.0 L min〈sup〉−1〈/sup〉 of air flow rate resulted in 11.97 mg L〈sup〉−1〈/sup〉 of IAA, an increase of 370% over original DYGS at 30 °C. Peat can still be regarded as a good cell carrier for solid state inoculants, whilst the additives tested for liquid formulations are individually more efficient than the mixture.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉The production of inoculants containing 〈em〉H. seropedicae〈/em〉 strain BR11471 can be efficiently improved with the use of the RSM approach i.e. it maximizes the production of biomass and indolic compounds, and reduces culture media components, both key factors for large-scale industrial production.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉We examined how mechanical management of invasive macrophyte, 〈em〉Typha × glauca〈/em〉 alters plant-soil interactions underlying carbon processes and nutrient cycling, which are important to wetland function but under-represented in restoration research.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉In the northern Great Lakes, we compared plant biomass, light transmittance, soil nutrient availability and carbon mineralization rates of 〈em〉Typha〈/em〉-dominated controls with 〈em〉Typha〈/em〉 stands harvested above the waterline (harvest) and at the soil surface (submerged harvest).〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Relative to controls, harvested stands had 50% less litter and twice as much light transmittance to the water surface after one year. However, 〈em〉Typha〈/em〉 stems re-grew, and soil nutrient availability rates were similar to controls. Submerged harvest eliminated 〈em〉Typha〈/em〉 litter and stems, and increased light transmittance through the water column. P and K soil availability rates were 70% greater with submerged harvest than in controls. Soil C mineralization rates were not affected by treatment (mean ± 1 SE; 40.11 ± 2.48 μg C-CO〈sub〉2〈/sub〉 and 2.44 ± 0.85 μg C-CH〈sub〉4〈/sub〉 g〈sup〉−1〈/sup〉 soil C hr.〈sup〉−1〈/sup〉), but were positively correlated with soil Fe availability.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉While submerged harvest effectively decreased invasive 〈em〉Typha〈/em〉 biomass after one year, it increased soil nutrient availability, warranting further examination of macronutrient cycling and export during invasive plant management.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉In this study, the effect of sulfation due to air pollution in the portals of the Ince Minareli Madrasa built of pyroclastic rocks and the Karatay Madrasas (Konya, Turkey) built of marble was investigated. Since the sulfur element in sulfate salts is represented as sulfate, the sulfur contour maps (SCM), obtained by transferring the portable X-ray fluorescence measurements onto the orthophotographs, also show the sulfated crust formation, distribution and anomaly regions on the portal surfaces. Additionally, the anomaly regions originating from the difference in thermal conduction between the sulfated crusts and portal surfaces on thermal camera images were determined, and they were observed to be compatible with the anomaly regions on SCM. However, the size of this correlation depends on the chemistry of the building stone and the thickness of the sulfated crusts. Then, it was determined that the anomaly regions on SCM were distributed in relation to the washing out of sulfated crusts by rainfall and capillary moisture content. Accordingly, portal surfaces were divided into three as the sheltered, washing and capillary regions. Consequently, it was demonstrated that the sulfation effect due to air pollution on historical building surfaces which have appropriate rock chemistry can be determined by non-destructive testing methods like using the SCM supported by infrared thermography. The fact that the method is simple and repeatable and is easily applicable to other buildings was considered as the most important success of the method.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉〈em〉Malus prunifolia〈/em〉 (Chinese name: Fu Ping Qiu Zi), a wild relative of cultivated apple (〈em〉Malus〈/em〉 x 〈em〉domestica〈/em〉 Borkh), is extremely resistant to drought compared with domesticated cultivars, such as ‘Golden Delicious’. However, the molecular mechanisms underlying drought resistance of 〈em〉M. prunifolia〈/em〉 have not been characterized. This study investigates a new regulatory mechanism to improve apple drought resistance.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉〈em〉M. prunifolia〈/em〉 and ‘Golden Delicious’ were each grafted on 〈em〉M. hupehensis〈/em〉 for gene expression analysis. The methylation level of the 〈em〉DREB2A〈/em〉 promoter was determined by bisulfite sequencing and ChIP-qPCR. Chromatin immunoprecipitation sequencing (ChIP-seq) was used to identify target genes of MpDREB2A in apple.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The exposure to drought stress stimulated the expression level of 〈em〉DREB2A〈/em〉 gene more than 100-fold in 〈em〉M. prunifolia,〈/em〉 but only 16-fold in ‘Golden Delicious’. This difference in gene expression could not be explained in terms of difference in leaf relative water content. Correspondingly, the methylation level of 〈em〉M. prunifolia DREB2A〈/em〉 (〈em〉MpDREB2A〈/em〉) promoter region was significantly reduced. Additionally, 〈em〉MpDREB2A〈/em〉 conferred enhanced drought resistance when ectopically expressed in 〈em〉Arabidopsis〈/em〉. Over 2800 potential downstream target genes of MpDREB2A were identified by ChIP-seq and these downstream genes have diverse potential functions related to stress resistance.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Methylation regulation in promoter of 〈em〉MpDREB2A〈/em〉 may contribute to the drought resistance of 〈em〉M. prunifolia〈/em〉.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Characterization of barley-tissue–colonization efficiency (〈em〉Hordeum vulgare〈/em〉 L.) by 〈em〉Paraburkholderia tropica〈/em〉 MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a pot-substrate system as an approach to understand their potential behavior as bioinput.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Culture-dependent techniques were implemented to quantify surface and endophytic bacterial populations in plant tissues. Culture-independent techniques were employed to detect and localize the inoculated bacteria in tissue samples along with evaluating the biofilm-forming capability by epifluorescent, confocal-laser-scanning, and scanning-electron microscopy. Plant-growth parameters were measured to evaluate the effects of the inoculated bacteria on the development of the barley plants.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉〈em〉Paraburkholderia tropica〈/em〉 grew as a biofilm on both abiotic and biotic surfaces and efficiently colonized barley roots and stems in plants grown in flasks in presence of other microorganisms. The bacteria was localized on root surfaces, hairs, and central-cylinder areas. 〈em〉Paraburkholderia tropica〈/em〉 also colonized the roots and stems in plants grown in the pot-substrate system. Although no endophytic root colonization occurred, the presence of the inoculated bacteria improved the aerial weight. A nested PCR detected 〈em〉P. tropica〈/em〉 in the tissue samples.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉〈em〉Paraburkholderia tropica〈/em〉 MTo-293 was characterized as an efficient biofilm-forming and barley-tissue–colonizing bacterium despite the presence of other microorganisms, but root endophytic colonization resulted dependent on the plant-growth system. Molecular-biology techniques were optimized, and also, its presence was correlated with plant-growth–promoting activity.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉In the face of problems caused by ‘intensive agriculture’ dominated by large areas of monocultures, mixed intercropping mimicking natural ecosystems has been reported to constitute a viable solution to increase and stabilize productivity. When designing such systems, root niche separation was thought to be a prerequisite to optimize production.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉This paper reviews the beneficial and adverse effects of trees and crops on water acquisition and redistribution in agroforestry ecosystems using the concepts of competition and facilitation between plants in link with root functional traits.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The results of the review showed that the reality was more complex leading agroforestry practitioners to adopt management practices to induce a separation in root activities thus avoid competition, particularly for water. Water uptake by plant roots is triggered by the water potential difference between the soil and the atmosphere when leaf stomata are open and depends largely on the root exploration capacity of the plant. Thus, root water uptake dynamics are strongly related to root-length densities and root surface areas. In addition, plants with deep roots are able to lift up or redistribute water to the upper layers through a process known as hydraulic lift, potentially acting as “bioirrigators” to adjacent plants. The redistributed water could be of importance not only in regulating plant water status, e.g. by enhancing transpiration, but also in increasing the survival and growth of associated crops in mixed systems.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Even though some more work is still needed to assess the volume of water transferred to neighbors, hydraulic lift could constitute an ecological viable mechanism to buffer against droughts and ensure productivity in regions with erratic rainfall. Giving the difficulty in measuring the above-mentioned aspects in the field, modeling of some of the most relevant parameters to quantify them might inform the design of future empirical studies.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Alpine ecosystems are important terrestrial carbon (C) pools, and microbial decomposers play a key role in cycling soil C. Microbial metabolic limitations in these ecosystems, however, have rarely been studied. The objectives of this study are to reveal the characteristics of microbial nutrient limitation, and decipher the drivers in the alpine ecosystems.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Models of extracellular enzymatic stoichiometry were applied to examine and compare the metabolic limitations of the microbial communities in bulk and rhizosphere soils along an altitudinal gradient (2800–3500 m a.s.l.) under the same type of vegetation (〈em〉Abies fabri〈/em〉) on Gongga Mountain, eastern Tibetan Plateau.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The soil microbial communities suffered from relative C and phosphorus (P) limitations in the alpine ecosystem despite of high soil nutrient contents here. Partial least squares path modelling (PLS-PM) revealed that the limitations were directly regulated by soil nutrient stoichiometry, followed by nutrient availability. The C and P limitations were higher at the high altitudes (3000–3500 m) than that at the low altitude (2800 m), which mainly attribute to changes of soil temperature and moisture along the altitudinal gradient. This suggested that global warming may relieve microbial metabolic limitation in the alpine ecosystems, and then is conducive to the retention of organic C in soil. Furthermore, the C and P limitations varied significantly between the bulk and rhizosphere soils at the high altitudes (3200–3500 m), but not at the low altitudes. This indicated the influences of vegetation on the microbial metabolisms, while the influences could decrease under the scenario of global warming.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Our study suggests that the alpine ecosystems with high organic C storage harbour abundant microbial populations limited by relative C and P, which have sensitive metabolic characteristics. This could thus potentially lead to large fluctuations in the soil C turnover under climate change. The study provides important insights linking microbial metabolisms to the environmental gradients, and improves our understanding of C cycling in alpine ecosystems.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Nitrogen (N) deposition affects litter decomposition. However, how nutrients, especially deposited N are immobilized and released in decomposing litters with different qualities (C/N and C/P) remains unclear.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We conducted a laboratory microcosm experiment with four litter types and a combination of a coniferous and deciduous litter treated with N addition (6 mg 99.99% 〈sup〉15〈/sup〉N g〈sup〉−1〈/sup〉 litter) and control by measuring N-deposition effect on mass (NDEM), N (NDEN), and P remaining percentages (NDEP), deposited 〈sup〉15〈/sup〉N immobilized abundance, and microbial composition and enzyme activities in decomposing litters during two years of incubation.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The values of NDEM, NDEN, and NDEP were generally greater for the litters with intermediate C/N and C/P than those with the highest and lowest ratios after 360 days, although these parameters varied among different quality litters before 180 days. Immobilized exogenous 〈sup〉15〈/sup〉N abundance by microbes showed an increasing trend with increasing litter C/N and C/P across the whole 720-day period. Both C/N and C/P were generally correlated with decomposition rate, 〈sup〉15〈/sup〉N immobilization abundance, the microbial richness, and main enzyme activities in decomposing litters.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉N-deposition effects on N and P dynamics in decomposing litters varied with their C/N and C/P, generally exerting an unimodal curve at later decomposition stages. Lower quality litter with higher C/N and C/P favoured N immobilization in response to N addition.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉The information of nitrogen uptake by subtropical, ever-green broad-leaf plants at cold temperatures of winter is very limited. The present field experiment was conducted to investigate whether 〈sup〉15〈/sup〉N is taken up by tea (〈em〉Camellia sinensis〈/em〉 L.) plants in winter dormancy in the absence of active shoot growth and utilization in young spring shoots.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We applied 〈sup〉15〈/sup〉N-labeled urea to soil at five different times i.e. mid-January, early February, mid-February, and early and mid-March. 〈sup〉15〈/sup〉N abundance was determined in fibrous roots, twigs and mature leaves after 3, 7 and 15 days after application and in young shoots the following spring.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉〈sup〉15〈/sup〉N was taken up by fibrous roots and transported to above-ground tissues within 3 days after application under low winter temperatures. Earlier application significantly increased nitrogen derived from 〈sup〉15〈/sup〉N-urea (N〈sub〉dff〈/sub〉) and 〈sup〉15〈/sup〉N amount in young spring shoots. N〈sub〉dff〈/sub〉 values and 〈sup〉15〈/sup〉N amount in young spring shoots were described well by quadratic or linear regressions against soil growing degree days (GDD, T ≥ 8 °C, depth 20 cm) between 〈sup〉15〈/sup〉N application and harvesting dates (R〈sup〉2〈/sup〉 = 0.58–0.90, 〈em〉p〈/em〉 〈 0.001).〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Nitrogen was absorbed and translocated in dormant tea plants in the absence of active root and shoot growth throughout the late winter until early spring. Absorbed N was stored and remobilized to support shoot growth the following spring. Soil GDD between N application and harvesting could predict N〈sub〉dff〈/sub〉 and 〈sup〉15〈/sup〉N amount in young spring shoots.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉To identify the rhizobia nodulating 〈em〉Vicia sativa〈/em〉 in Northwestern China and to estimate their geographic distribution.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Rhizobia trapped with 〈em〉V. sativa〈/em〉 plants from soils at six sites in the northwest of China were classified into genotypes by PCR-based restriction fragment length polymorphism (RFLP) of 16S–23S rRNA intergenic spacer (IGS) and 16S rRNA genes, and phylogenetic analyses of housekeeping (16S rRNA, 〈em〉recA〈/em〉, 〈em〉atpD〈/em〉) and symbiotic genes were performed for the representative strains. Soil physicochemical characteristics were recorded and canonical correlation analysis was performed to examine the correlations between soil features and distribution of rhizobial genotypes.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉A total of 202 rhizobial isolates were discriminated by RFLP analyses into 15 IGS types and a single 16S rRNA type, which were identified as 〈em〉Rhizobium〈/em〉 by 16S rRNA gene phylogeny and as four clusters by multilocus sequence analysis (MLSA). Cluster 1 covering 86 strains and 7 IGS types prevalent in all sites was identified as 〈em〉Rhizobium laguerreae〈/em〉; cluster 2 was 〈em〉R. sophorae〈/em〉 with 18 strains in 2 IGS types found in the site Q-GD. Each of cluster 3 and cluster 4 contained three IGS types representing two novel 〈em〉Rhizobium〈/em〉 genospecies specific to Gansu Province and Shanxi Province, respectively. Four 〈em〉nodC〈/em〉 phylogenetic clades were defined among the isolates.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉〈em〉R. sophorae〈/em〉, 〈em〉R. laguerreae〈/em〉, and two novel 〈em〉Rhizobium〈/em〉 genospecies with diverse symbiotic genotypes are associated with 〈em〉Vicia sativa〈/em〉 L. in Northwest China. Their biogeographic patterns are mainly directed by soil pH and salinity. This is the first study on the diversity of rhizobia nodulating 〈em〉Vicia sativa〈/em〉 in China.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Previous basalt carbonation studies under hydrothermal-like conditions suggested high amount of calcite formation in a shorter period which is related to the andesine dissolution. However, CO〈sub〉2〈/sub〉 capture-induced structural changes in neo-formed minerals have not been studied in detail. To understand these changes, Rietveld refinement and crystallographic characterization were performed for andesine and calcite residue obtained after basalt–water–CO〈sub〉2〈/sub〉 interaction experiments. These results indicate large spontaneous strain within calcite which led to contraction along the 〈em〉c〈/em〉-axis and expansion along 〈em〉a〈/em〉-axis. Thus, contraction/expansion along the 〈em〉c〈/em〉-axes is inversely proportional to 〈em〉a〈/em〉-axis of the calcite and andesine, respectively. Interatomic Ca–O and C–O bond lengths show changes with the experimental run time, signifying mutually inverse relationship. Observed crystal structure distortion is due to variation in the O–M–O bond angle. Ionic bond length between Ca and O atoms decreased after 70 h of experiment run that enabled Ca–O ligand to enter in the phyllosilicates. The Ca–O bond length weakening along 〈em〉c〈/em〉-axis caused shrinkage of calcite crystals. Increased bond length together with the weakened covalent bond between C and O atoms led to calcite degeneration after 80 h of experiments; therefore, no calcite persisted in the system. Thus, basalt carbonation is time-dependent process, where, 〈em〉p〈/em〉CO〈sub〉2〈/sub〉 plays sub-ordinate role, but, at a constant temperature.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aim〈/h3〉 〈p〉Root-associated microbial communities influence plant phenotype, growth and local abundance, yet the factors that structure these microbial communities are still poorly understood. California landscapes contain serpentine soils, which are nutrient-poor and high in heavy metals, and distinct from neighboring soils making them ideal for studying the factors that structure root microbiomes and their functions.〈/p〉 〈/span〉 〈span〉 〈h3〉Method〈/h3〉 〈p〉Here, we surveyed the rhizoplane of serpentine-indifferent plants, which grow on and off serpentine soil, to determine the relative influence of plant identity and soil chemistry on rhizoplane microbial community structure using 16S rRNA metabarcoding. Additionally, we experimentally examined if serpentine vs. non-serpentine microorganisms differentially affected plant growth in serpentine soil.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Rhizoplane bacterial communities differed among plant species, soil types, and the interaction between them in both the field and experimental soils. In the experiment, soil microbial community source influenced seedling survival, but plant growth phenotypes were largely invariant to microbial community with a few exceptions.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Rhizosplane bacterial species composition differed between plant species and soil types, and Amplicon Sequence Variants (ASVs) from the phyla Acidobacteria and Proteobacteria (Genus: 〈em〉Microvirga〈/em〉) were characteristic of serpentine soils. While soil microbial community composition influenced seedling survival in the current study, further study is required to disentangle the role of microbial associations and plant tolerance to serpentine.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Plants can optimize the allocation of phosphorus (P) among their foliar P fractions to increase the P utilization efficiency (PUE). Identifying the genetic relationships between foliar P fractionation and PUE could provide opportunities to improve the P efficiency of barley (〈em〉Hordeum vulgare〈/em〉 L.).〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉The differences in the concentrations and proportions of inorganic P, ester P, nucleic acid P and insoluble P between a wild barley cultivar CN4027 and a commercial cultivar Baudin were studied, and their quantitative trait loci (QTLs) at normal P (NP) and low P (LP) fertilisations were mapped. The PUE that was determined in the previous study was used to analyze the relationship between PUE and foliar P fractionation in this research.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Both cultivars of barley could increase their metabolic P fractions as an LP stress tolerance strategy to ensure their continued metabolic activities in response to LP stress. Cultivar CN4027 showed higher nucleic acid P concentration (NPC), nucleic acid P proportion (NPP) and insoluble P proportion (IPP) than cultivar Baudin under LP stress. This abundant organic P (Po) pool of CN4027 ensured the normal functioning of its metabolic pathways under LP stress. The close relationships between the foliar P fractionation and PUE could be explained by two QTL clusters, 〈em〉Cl-3H.02〈/em〉 and 〈em〉Cl-5H.01〈/em〉. The QTL cluster 〈em〉Cl-3H.02〈/em〉 is flanked by the markers 〈em〉bPb3256099-bPb3255630〈/em〉 on chromosome 3H and controls the ester P concentration (EPC), ester P proportion (EPP), insoluble P concentration (IPC) and IPP.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉The QTL cluster 〈em〉Cl-3H.02〈/em〉 might have great potential for the future genetic improvement of barley PUE and may offer clues for the genetic relationships between the foliar P fractionation and PUE.〈/p〉 〈/span〉

Beschreibung: 〈p〉The authors would like to correct errors in the original publication as detailed below.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Roots are vital organs for plants, but the assessment of root traits is difficult, particularly in deep soil layers under natural field conditions. A popular technique to investigate root growth under field or semi-field conditions is the use of minirhizotrons. However, the subsequent manual quantification process is time-consuming and prone to error.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We developed a multispectral minirhizotron imaging system and a subsequent image analysis strategy for automated root detection. Five wavelengths in the visible (VIS) and near-infrared (NIR) spectrum are used to enhance living roots by a multivariate grouping of pixels based on differences in reflectance; background noise is suppressed by a vesselness enhancement filter. The system was tested against manual analysis of grid intersections for both spring barley (〈em〉Hordeum vulgare L.〈/em〉) and perennial ryegrass (〈em〉Lolium perenne L.)〈/em〉 cultivars at two time-points. The images of living roots were captured in wet subsoil conditions with dead roots present from a previous crop.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Under the soil conditions used in the study, NIR reflectance (940 nm), provided limited ability to separate between rhizosphere components, compared to reflectance in the violet and blue light spectrum (405 nm and 450 nm). Multivariate image analysis of the spectral data, combined with vesselness enhancement and thresholding allowed for automated detection of living roots. Automated image analysis largely replicated the root intensity found during manual grid intersect analysis of the same images. Although some misclassification occurred, caused by elongated structures of dew and chalkstone with similar reflectance pattern as living root, the system provided similar or in some cases improved detection of genotypic differences in the total root length within each tube.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉The multispectral imaging system allows for automated detection of living roots in minirhizotron studies. The system requires considerably less time than traditional manual recording using grid intersections. The flexible training strategy used for root segmentation offers hope for the transfer to other rhizosphere components and other soil types of interest.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉The objective of this research was to develop a three-dimensional (3D) rhizosphere modeling capability for plant-soil interactions by integrating plant biophysics, water and ion uptake and release from individual roots, variably saturated flow, and multicomponent reactive transport in soil.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We combined open source software for simulating plant and soil interactions with parallel computing technology to address highly-resolved root system architecture (RSA) and coupled hydrobiogeochemical processes in soil. The new simulation capability was demonstrated on a model grass, 〈em〉Brachypodium distachyon〈/em〉.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉In our simulation, the availability of water and nutrients for root uptake is controlled by the interplay between 1) transpiration-driven cycles of water uptake, root zone saturation and desaturation; 2) hydraulic redistribution; 3) multicomponent competitive ion exchange; 4) buildup of ions not taken up during kinetic nutrient uptake; and 5) advection, dispersion, and diffusion of ions in the soil. The uptake of water and ions by individual roots leads to dynamic, local gradients in ion concentrations.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉By integrating the processes that control the fluxes of water and nutrients in the rhizosphere, the modeling capability we developed will enable exploration of alternative RSAs and function to advance the understanding of the coupled hydro-biogeochemical processes within the rhizosphere.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The application of salt–bentonite (Salt–Bent) grouting for the design and construction of grout curtain to form vertical barriers (VB) to serve as seepage remediation measures has been investigated. Recent experiences on the high cost of grout injection materials when conducting a grouting campaign have necessitated the authors to consider the use of clay of the montmorillonite (Mt) type (Wyoming bentonite), which is less expensive; readily available naturally occurring; yet very effective and environmentally friendly grouting material for the construction of grout curtain. Grout curtain is formed by injecting grout slurry into a series of boreholes, drilled through the weathered zone of the subsurface, to form VB. Vertical barriers play a significant role in geo-environmental engineering for the control of horizontal groundwater flow and contaminant spread into the unsaturated–saturated zone of the subsurface. The design and construction of effective grout curtain is key to the overall performance of the barrier and this is dependent on the accurate determination of a design parameter, the critical hydraulic gradient (CHG). A laboratory investigation of the CHG was conducted through grout injection into fractures of aperture sizes 100–60 µm and river sand compacted to different porosities (0.33 ≤ 〈em〉n〈/em〉 ≤ 0.40), to determine the safety thickness of a bentonite-grout curtain to withstand a given hydraulic gradient without failure. The study finds that, the CHG due to the fractured and porous media ranges between 30 and 35; and 180 and 200, respectively. The resulting permeability values obtained from the grouted samples were in the order of 10〈sup〉−7〈/sup〉cm/s or less. It can be inferred from the results that, bentonite-grouting can provide the required permeability for an effective barrier. The results also suggest that, accurate determination of the safety thickness of the barrier is of great importance for sustainable barrier system.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The transport and fate of hexavalent chromium Cr(VI) in slag–soil system, which remain poorly understood, are of great importance for environmental risk assessment and pollution control of Cr(VI) in soil and groundwater. Based on the migration behavior of Cr(VI) in the chromium-containing slag under the effects of rainfall, the transport and fate of Cr(VI) in soil were investigated. Batch and column experiments were undertaken to clarify the Cr(VI) adsorption and transport behavior of aqueous Cr(VI) in soil, respectively. Findings from a combination of experimental and model results clearly suggest that the adsorption of Cr(VI) onto soil is a spontaneous chemical adsorption process. For chromium-soil transport system, the constructed HYDRUS-1D dynamics model is a good simulation for Cr(VI) migration in soil, as the modeling yielded adequate fit for simulated and measured values of Cr(VI) concentration in soil (correlation coefficient 〈em〉R〈/em〉〈sup〉2〈/sup〉 = 0.99). The transport modeling then was utilized to assess the transport of Cr(VI) in slag–soil system. Up to the year of 2018, the concentration levels of Cr(VI) in the soil-leaching solution attained 905 mg/L. Due to the thin layer of soil and the high mobility of Cr(VI) in soil, it is no doubt that the Cr(VI) leaching will engender serious pollution of soil and groundwater in the presence of precipitation. The applications and limitations of the HYDRUS-1D were also discussed, such as bacteria transport, more elaborate biogeochemical processes, coupling of vadose zone processes with existing larger scale groundwater flow models.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Influenced by the uncertainties and differences of the variations of precipitation abundance, the water source area of a water transfer project is different from the water receiving area, which will lead to risks for the operation of the water transfer project. In this article, expressions for the exceedance probabilities of wet vs. dry conditions were constructed on the basis of the copula joint distribution function. The flood season, non-flood season, and annual precipitation data from the Jvxian and Rizhao stations of the Shuhe to Futuan Water Transfer Project (SFWTP) were used for the analysis of synchronous and asynchronous exceedance probabilities. The computed results demonstrate that synchronous exceedance probabilities were greater than asynchronous exceedance probabilities via the analysis of the flood season, non-flood season, and annual precipitation, and the favorable probability is largest during the flood season. In order to maximize the SFWTP benefit, it is essential to increase urban water supply in the non-flood season, and transport water in the flood season to Rizhao reservoir. To further analyze the impacts of climate change on exceedance probability, the precipitation data were divided into two sub-periods by Pettitt test. In last sub-period, favorable probabilities increased with the decrease of synchronous exceedance probability in all timescales, which suggests that there are positive impacts of climate change on the exceedance probabilities.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉To investigate the effects of flow rate variation on solute transport in a karst conduit, three pipe structures of a constant diameter pipe, the pipe connected to a symmetrical pool and an asymmetrical pool respectively were chosen, and several tracer experiments were conducted separately in each of the three pipe structures at nine flow rates. Experimental results show that the peak of the breakthrough curve (BTC) increased and the tailing decreased with increasing discharge. Three models, the advection–dispersion equation (ADE), the two-region nonequilibrium model (TRNM) and the transient storage model (TSM), were used to simulate BTCs and explore the change of transport parameters with increasing flow rate. Simulations show that ADE was capable of replicating the almost symmetrical BTCs of the single pipe but incapable of fitting the appreciable BTC tails for the pools. Nevertheless, TRNM and TSM can reproduce all BTCs of single pipe and pipe with a pool very well. The research demonstrates the significant effect of the pool on solute transport. The parameters in the two models (TRNM and TSM) exhibited similar trends with increasing discharge in either pool. In the TRNM, a clear positive correlation with discharge emerged for the partition coefficient and mass transfer coefficient. Meanwhile, the main channel cross-sectional area and exchange coefficient in TSM increased gradually with discharge. The storage zone area decreased generally with increasing flow rate. The relationship between solute transport and the flow rate is more complex in the asymmetrical pool than in the symmetrical pool.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉As a major plant-derived soil organic carbon (SOC) component, lignin-derived phenolic compounds show varying biogeochemical characteristics compared to plant-derived lipid moieties. Comparing their distribution patterns can provide information on mechanisms governing SOC preservation and dynamics. However, the large-scale distribution pattern and stability of lignin versus plant-derived lipids are still poorly constrained. Here we investigated the distribution of lignin phenols versus plant-derived lipids in the surface soils across the alpine versus temperate grasslands of China and Mongolia.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Lignin phenols were isolated by cupric oxide oxidation method and compared with the previously analyzed plant-derived lipids (cutin and suberin). A comprehensive list of environmental variables was compiled to disentangle the climatic, edaphic and vegetation influences on lignin phenols’ distribution in the soil.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Lignin phenols showed similar SOC-normalized concentrations in the alpine and temperate grassland soils despite a higher plant input to the latter, suggesting better lignin preservation in the cold region. However, compared with plant-derived lipids (cutin and suberin), lignin seems to be less stabilized. The variation of lipid versus lignin components is mainly related to climate (particularly aridity) in the alpine grassland soils, while the relative abundance of plant lipids and lignin phenols is more related to reactive mineral contents in the temperate grassland soils.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Lignin contributes differentially to SOC accumulation in the alpine and temperate soils: while lignin seems to be better preserved in the cold region, lignin phenols decrease relative to other carbon components with SOC accrual in the temperate region. Overall, lignin distribution and fate may be more sensitive to carbon source variations than temperature shifts in the grasslands.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉A century of atmospheric deposition of sulfur and nitrogen has acidified soils and undermined the health and recruitment of foundational tree species in the northeastern US. However, effects of acidic deposition on the forest understory plant communities of this region are poorly documented. We investigated how forest understory plant species composition and richness varied across gradients of acidic deposition and soil acidity in the Adirondack Mountains of New York State.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We surveyed understory vegetation and soils in hardwood forests on 20 small watersheds and built models of community composition and richness as functions of soil chemistry, nitrogen and sulfur deposition, and other environmental variables.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Community composition varied significantly with gradients of acidic deposition, soil acidity, and base cation availability (63% variance explained). Several species increased with soil acidity while others decreased. Understory plant richness decreased significantly with increasing soil acidity (〈em〉r〈/em〉 = 0.60). The best multivariate regression model to predict richness (〈em〉p〈/em〉 〈 0.001, adjusted〈em〉-R〈/em〉〈sup〉〈em〉2〈/em〉〈/sup〉 = 0.60) reflected positive effects of pH and carbon-to-nitrogen ratio (C:N).〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉The relationship we found between understory plant communities and a soil-chemical gradient, suggests that soil acidification can reduce diversity and alter the composition of these communities in northern hardwood forests exposed to acidic deposition.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Endophytic fungi colonization is an eco-friendly strategy to respond to environmental stresses and confer tolerance to the host plant. Here, the responses of wheat plant inoculated with an indole acetic acid (IAA) -producing endophytic fungus to drought stress and water recovery were evaluated.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉The inoculation of wheat plants with 〈em〉Alternaria alternata〈/em〉 (LQ1230) was conducted to evaluate drought resistance under adequate water, water deficit and water recovery conditions by examining the growth parameters and various physiological indicators of wheat seedlings.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The LQ1230 isolated from 〈em〉Elymus dahuricus〈/em〉 Turcz could secrete indole acetic acid (IAA) by both the tryptophan-dependent (319.24 ± 14.88 μg/mL) and independent (40.12 ± 8.59 μg/mL) pathways. LQ1230 inoculation enhanced wheat growth and drought tolerance through regulation of antioxidant enzyme activities and the content of compatible solutes such as soluble sugars and proline. Additionally, LQ1230 inoculated plants demonstrated significantly improved photosynthesis, C and N accumulation of wheat plants, leading to a positive relationship with plant dry biomass under water deficit and re-watering conditions.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉We found that the improved wheat plant growth, photosynthesis and nutrient accumulations by the inoculation of 〈em〉Alternaria alternata〈/em〉 LQ1230 might be attributed to the reprogramming of wheat plant metabolism, thus enhancing wheat drought tolerance. Inoculation with fungal endophytes such as LQ1230 has the potential to increase crop drought resistance.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Subtropical ecosystems are generally characterized by phosphorus (P)-deficient soils; however, extreme P-rich soils develop on phosphate rocks. We aimed to integrate metabolomic and ionomic analyses to survey how in situ trees adaptively respond to such contrasting P soils.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Gas (GC-MS) or liquid (LC-MS) chromatography-mass spectrometry and inductively coupled plasma-optical emission spectrometer (ICP-OES) were used to analyze leaf metabolome and ionome of 〈em〉Quercus variabilis〈/em〉, which grew at two geologic P-rich and P-deficient sites in subtropical China.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Two 〈em〉Q. variabilis〈/em〉 populations were significantly discriminated in terms of metabolome and ionome, with major contributions from 25 identified metabolites (e.g. sugars and P-containing compounds) and P and four other chemical elements. And of these 25 metabolites, orthophosphate was predominant in influencing the variation in the metabolomes of 〈em〉Q. variabilis〈/em〉 between the two P-type sites. Moreover, orthophosphate was correlated with leaf P (〈em〉r〈/em〉 = 0.85, 〈em〉p〈/em〉 〈 0.001), while leaf P was significantly influenced only by soil resident P at the P-rich site. Furthermore, the metabolic pathway analysis indicated four critical metabolic pathways: galactose metabolism, amino sugar and nucleotide sugar metabolism, glyoxylate and dicarboxylate metabolism, fructose and mannose metabolism.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉These findings suggested that there were distinct ionome-metabolome interactions in 〈em〉Q. variabilis〈/em〉 populations, between P-rich and P-deficient sites, which contributed to novel insights into how plants interactively adapt to P-limiting soils.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The optimal operation of an interbasin water transfer multireservoir system (IWTMS) is more complicated than a general one-basin multireservoir system because water release and water transfer occur simultaneously. Previous studies primarily employed reservoir zoning and rule curves to deal with the relationship between reservoir water release and water transfer, and the operating priorities for water release and water transfer are established by decision-makers. However, these priorities may be somewhat subjective and result in suboptimal operation of the entire system. To overcome this shortcoming, a genetic algorithm (GA)-based optimization model was developed to endeavor to get the optimal operation of an IWTMS. The optimization model consists of a GA and a simulation process based on water balance equation, and was applied to a typical IWTMS in Rizhao City, Shandong Province, China. The results showed that the GA was effective and efficient on solving the optimization problem of IWTMSs, and the reliability of the system’s water supply increased from 70.6 to 88.4% due to water transfer and could increase further to 96.4% by implementing optimization.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Although nitrogen (N) fertilization is widely used to increase rice yield, its impact on the distribution, transformation, and fates of photosynthetic carbon (C) in rice–soil systems is poorly understood. To address this, we quantified the C flows into various pools in a rice–soil system.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Rice (〈em〉Oryza sativa〈/em〉 L.) was pulse-labeled with 〈sup〉13〈/sup〉CO〈sub〉2〈/sub〉 at the tillering stage. Samples were collected six times during the 26 days following labeling. We quantified the partitioned photosynthesized C into various pools using stable isotopic techniques and estimated C flows.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Although the net distribution of assimilated C to belowground pools did not change, N fertilization promoted C assimilation in aboveground biomass. C allocation into soil was enhanced by N fertilization during early growth, but decreased during late growth. N fertilization induced higher mass-specific rhizodeposition (per unit root dry weight) and its turnover rate compared with the unfertilized system. However, with higher microbial turnover, the daily C allocation from roots to soil was similar at both fertilization levels.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Although total C input into soil is enhanced by N fertilization, its further fate is N fertilization independent, thus leading to a net accumulation of C input in rice paddy soil similar to that observed unfertilized soil.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Long-duration drought can alter ecosystem plant species composition with subsequent effects on carbon cycling. We conducted a rainfall manipulation field experiment to address the question: how does drought-induced vegetation change, specifically shrub encroachment into grasslands, regulate impacts of subsequent drought on soil CO〈sub〉2〈/sub〉 efflux (R〈sub〉s〈/sub〉) and its components (autotrophic and heterotrophic, R〈sub〉a〈/sub〉 and R〈sub〉h〈/sub〉)?〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We conducted a two-year experiment in Inner Mongolia plateau, China, using constructed steppe communities including graminoids, shrubs and their mixture (graminoid + shrub) to test the effects of extreme-duration drought (60-yr return time) on R〈sub〉s〈/sub〉, R〈sub〉h〈/sub〉 and R〈sub〉a.〈/sub〉〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Our results indicated that extreme-duration drought reduced net primary production, with subsequent effects on R〈sub〉s〈/sub〉, R〈sub〉h〈/sub〉 and R〈sub〉a〈/sub〉 in all three vegetation communities. There was a larger relative decline in R〈sub〉a〈/sub〉 (35–54%) than R〈sub〉s〈/sub〉 (30–37%) and R〈sub〉h〈/sub〉 (28–35%). Interestingly, we found R〈sub〉s〈/sub〉 in graminoids is higher than in shrubs under extreme drought. Meanwhile, R〈sub〉h〈/sub〉 declines were largest in the shrub community. Although R〈sub〉a〈/sub〉 and R〈sub〉h〈/sub〉 both decreased rapidly during drought treatment, R〈sub〉h〈/sub〉 recovered quickly after the drought, while R〈sub〉a〈/sub〉 did not, limiting the 〈em〉R〈/em〉〈sub〉〈em〉s〈/em〉〈/sub〉 recovery.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉This study suggests that plant species composition regulates several aspects of soil CO〈sub〉2〈/sub〉 efflux response to climate extremes. This regulation may be limited by above- and below-ground net primary production depending on soil water availability〈em〉.〈/em〉 The results of this experiment address a critical knowledge gap in the relationship between soil respiration and plant species composition. With shrub encroachment into grasslands, total soil respiration is reduced and can partly offset the effect of reduction in productivity under drought stress.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Humans are daily exposed to the natural radioactivity present in rocks, soils, and water. The distribution of these elements in the surface is not uniform, being influenced by the variation of the physical, geological, and meteorological parameters. The concentration activities of natural radionuclides 〈sup〉238〈/sup〉U, 〈sup〉226〈/sup〉Ra, 〈sup〉228〈/sup〉Ra, and 〈sup〉222〈/sup〉Rn were determined in the groundwater supplying the Salutaris Mineral Waters Park, in Paraíba do Sul, in the state of Rio de Janeiro. The concentrations of 〈sup〉238〈/sup〉U varied from 0.95 to 2.70 μg L〈sup〉−1〈/sup〉 with a mean concentration of 1.96 μg L〈sup〉−1〈/sup〉, 〈sup〉226〈/sup〉Ra ranged from 1.50 to 12.6 mBq L〈sup〉−1〈/sup〉 with an average of 5.03 mBq L〈sup〉−1〈/sup〉, 〈sup〉228〈/sup〉Ra presented levels between 1.80 and 2.80 mBq L〈sup〉−1〈/sup〉, with an average of 2.40 mBq L〈sup〉−1〈/sup〉, and 〈sup〉222〈/sup〉Rn, with levels of 5.90–1.94 × 10〈sup〉4〈/sup〉 mBq L〈sup〉−1〈/sup〉 with an average concentration of 7.50 Bq L〈sup〉−1〈/sup〉. The contribution of the consumption of these radionuclides dissolved in the water distributed in the Park to the effective annual dose ranged from 0.03 to 0.10 mSv year〈sup〉−1〈/sup〉, with an average of 0.08 mSv year〈sup〉−1〈/sup〉. The results showed that all effective annual dose values per ingestion of these mineral waters were below the individual dose limit of 0.10 mSv year〈sup〉−1〈/sup〉 recommended by the World Health Organization (WHO).〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉The effects of root glutathione (GSH) supplementation on leaf chlorophyll, Fe concentrations and contents in leaves, stems and roots, and traits associated to Fe deficiency were studied in 〈em〉Medicago scutellata〈/em〉 plants grown in rock sand under conditions of Fe deficiency, in the presence of different concentrations of bicarbonate.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Plants were grown in acid-washed rock sand irrigated with a zero Fe solution (pH 7.8 with 0.5 g L〈sup〉−1〈/sup〉 CaCO〈sub〉3〈/sub〉) or a 45 μM Fe(III)-EDDHA solution (5 mM MES, pH 5.5), with 0, 5 or 15 mM NaHCO〈sub〉3〈/sub〉, and 250 mL of 1 mM GSH was added daily to half of the pots.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Iron deficiency caused characteristic symptoms in plants, with GSH supplementation relieving them. Glutathione supplementation led to increases in total Fe, chlorophyll and leaf total and extractable Fe, whereas root Fe concentrations decreased. Traits associated to Fe deficiency, including changes in biomass, root morphology, carboxylate contents and antioxidant parameters became less intense with GSH supplementation.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Glutathione supplementation allowed plants to take up Fe from the rock sand via a reductive solubilization mechanism. Also, the distribution of Fe within the plant changed, with more Fe being allocated to the shoot tissues and less to the roots.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉An attempt to understand the structural and sedimentation framework in the Benin Basin is made herein. Unlike in its contiguous basins in the Gulf of Guinea Province, no significant oil and gas discovery has been reported in the basin. The aim here is to provide a chronostratigraphic framework of sediments build-up as a guide to hydrocarbon prospectivity in the basin. Existing biostratigraphic and sedimentologic data as well as few proprietary reports were integrated to establish a chronostratigraphic correlation in six directions within the Nigerian portion of the Benin Basin. Isopach maps of the chronostratigraphic subdivision of the sequences in the basin were also made. Sedimentation in restricted grabens started in the western part of the basin. Only Ise-2 well penetrated the oldest Cretaceous (Neocomian to Albian) sediments in the area of study with a thickness reaching up to 869 m. During the Miocene the focus of sedimentation predominantly shifted towards the offshore in the basin. Significantly a missing Eocene/Oligocene section was observed both on the seismic section and the stratigraphic record of the basin. Only Epiya-1 shows gas occurrence in the Miocene. Based on this work and locations of hydrocarbons recovery in the other part of the basin, exploration effort should target the Cretaceous sediments in the Nigerian portion of the basin.〈/p〉

Beschreibung: 〈p〉Unfortunately the online version of the original article contains several citations of Jannis et al. 2018, where the first and last name was accidently mix up.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Floodwater resource utilization, a new term or concept was proposed in the early 21st century in China, and has been practiced over the past 20 years in order to adapt to the uncertain effects of climate change on regional droughts and water scarcity. The applications shown that the effect was good and necessary to alleviate the regional water shortage. This special thematic issue collected 14 articles covering three continents—Asia, America and Europe in term of research area, which come down to basic concepts, evaluation methods, utilization models and usable patterns and so on. Through this thematic issue, the lessons, experiences, and achievements on floodwater resources utilization achieved mainly in China can be shared with world audiences, especially to regions affected by the monsoon climate, with similar hydrologic characteristics and water resource needs as in China.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Mycorrhizae and root exudates have been considered the two important pathways for nitrogen (N) transfer from legume to non-legume plants. The present study aimed to investigate contribution of the relative importance of arbuscular mycorrhizal fungi and root exudates in short-term N transfer.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉A field experiment was conducted to explore N transfer from alfalfa to maize under two different N application levels using 〈sup〉15〈/sup〉N leaf labeling.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉N transfer amount ranged from 7 to 10 mg N plant〈sup〉−1〈/sup〉 from alfalfa to maize and significantly decreased (by 11%–22%) with N fertilizer application. Intercropping of 4 rows of maize and 6 rows of alfalfa with 30 cm intra-row spacing (IMA43) was the optimal intercropping mode, which increased N transfer, total N uptake and yield by 18%, 15% and 11%, respectively. The relative importance of arbuscular mycorrhizal fungi and root exudates on N transfer was dependent on soil N availability. Under no N addition, hyphal length density (HLD) of rhizosphere soil explained the largest significant amount (50%) of the variability in N transfer and crop yield. However, root exudates explained 77% of the variability in N transfer and crop yields with N fertilizer application.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Our findings highlighted that N transfer is reliant more on arbuscular mycorrhizal fungi than root exudates in N-deficient soil, whereas root exudates play a more important role in N-fertilized soil.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The microcrack propagation-coalescence mechanism and stress–strain constitutive model of brittle rock is of great significance for prediction of mechanical properties. To establish a more applicable constitutive model of brittle rock, the crack model based on fracture mechanics was introduced. First, based on the crack slip model and maximum circumferential stress theory, a theoretical equation for crack propagation length was established. The microcrack propagation-coalescence mechanism of brittle rock material under loading was studied. On this basis, a macro-coalescence zone model was proposed. Through the relationship between the deformation characteristics of stress–strain curves at different crack growth stages and the behavior of microcracks, a complete stress–strain constitutive model of brittle rock based on the crack model was theoretically deduced. The constitutive model was verified by the results of a coupling hydro-mechanical test of granite carried out after different heat treatment temperatures. The advanced intelligent algorithm, genetic algorithm, was adopted to simulate experimental data. The research results showed that: (1) The derived stress–strain constitutive model of brittle rock is not only high in accuracy of stress–strain simulation, but also can simulate both the axial and circumferential stress–strain curves. Moreover, the dilatancy and crack initiation stresses can be obtained directly. (2) The parameters of the stress–strain constitutive model not only have clear physical meaning, but the mechanisms of rock mechanical properties, such as dilatancy, acoustic emission, and stress fluctuation, can be explained.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Magnesium (Mg) deficiency impacts many metabolic processes in 〈em〉Brassica napus〈/em〉 (〈em〉B. napus〈/em〉), leading to yield loss. However, the mechanism of Mg〈sup〉2+〈/sup〉 uptake and translocation in 〈em〉B. napus〈/em〉 remains unknown.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉After screening 39 genotypes of 〈em〉B. napus〈/em〉 under Mg-deficient conditions, the cultivars P160 and P153 were selected according to their Mg transfer factor (TF) from root to shoot. We further characterized these two genotypes under Mg-deficiency by analyzing chlorophyll concentration, malondialdehyde and peroxidase activity, and reducing sugar concentration in leaves. Additionally, we performed transcriptomics and qRT-PCR assays on P153 and P160 shoots and roots. The identified functional genes involved in Mg transport were characterized by functional assays in yeast and Arabidopsis mutants.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The physiological analysis revealed that P160 (Mg tolerance cultivar; Mg-T) is more tolerant than P153 (Mg sensitive cultivar; Mg-S) under magnesium-deficient environments. Transcriptomics and qRT-PCR assays revealed that transcript levels of 〈em〉BnMGT1–2〈/em〉 and 〈em〉BnMGT6–1〈/em〉 were more significantly up-regulated in the shoot of Mg-T cultivar than that of the Mg-S cultivar under Mg limitation. Functional assays of BnMGT1–2 and BnMGT6–1 reveals that BnMGT1–2 and BnMGT6–1 are the two main functional Mg transporters mediating Mg translocation from root to shoot under low Mg conditions.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉Mg-T is more efficient in the translocation of Mg from root to shoot than Mg-S. BnMGT1–2 and BnMGT6–1 should be the two main Mg transporters associated with Mg translocation under Mg deficiency condition, which caused the different Mg efficiency between the Mg-T and Mg-S.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The GlobalTreeSearch database provides the names of all tree species known to science and the countries where these trees grow. TreeeX is a visual exploration system that supports multifaceted analyses of the GlobalTreeSearch data. Investigating research questions on biodiversity and conservation on a global or national scale are visually supported by interactive choropleth maps that color countries according to frequency, diversity or uniqueness of prevalent tree species. By combining the GlobalTreeSearch and ThreatSearch data sets, additional information on the conservation status of trees can be visualized globally and nationally through TreeeX. Similarities and differences in tree diversity, endemism and conservation status to other countries can be analyzed in detail. Several examples outline the system’s capability of delivering insights concerning the geographical diversity of tree species. 〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Global climate change is characterized by enhanced atmospheric carbon dioxide concentration ([CO〈sub〉2〈/sub〉]) and temperature, with unknown consequences for soil nematode communities. Soil nematode in response to elevated [CO〈sub〉2〈/sub〉], warming and their interaction in paddy field remain largely unknown. Here we aimed to understand how factorial combinations of elevated [CO〈sub〉2〈/sub〉] and canopy warming affect soil nematode in a rice paddy field.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉A rice paddy field was consistently treated with elevated [CO〈sub〉2〈/sub〉] (500 ppm), canopy warming (+2 °C) or their combinations. Soil samples after a two-year treatment were collected during the rice growing season and nematode communities were extracted with a modified Baermann funnel extraction to examine the changes in nematode abundance and composition under climate change.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Soil nematode communities were altered by elevated [CO〈sub〉2〈/sub〉] and warming, but these responses were dependent on rice growing stages. When averaged over the four stages, total nematode abundances were increased by 31.5% under elevated [CO〈sub〉2〈/sub〉], and by 25.7% under warming. Elevated [CO〈sub〉2〈/sub〉] had no effect on nematode diversity, but slightly altered the composition of different trophic groups. In contrast, warming decreased nematode diversity, but increased plant parasite index, which was negative correlated with crop production. This was attributed to increases in the relative abundance of herbivores under simulated climate change conditions.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Elevated [CO〈sub〉2〈/sub〉] and warming had a positive effect on nematode abundance, but potentially reduced nematode diversity and soil health. These results suggest that multi-factors interactively affect the responses of soil nematode communities, which is important for food productivity under climate change.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Numerous groynes had been constructed on the Dwarkeswar River to improve bank protection. Among them, groynes adjacent to the village Rautara of Khandaghosh Block, West Bengal have been studied which were constructed in the year of 2009. This study investigates the alteration of channel morphology, sedimentology and flow characteristics influenced by emerged groynes through micro-level field study. An extensive field survey has been made with a dumpy level and fifty-nine sediment samples were collected from the field. Different channel parameters such as degradation aggradation ratio, braided index, channel instability and bar occupied area indices have been used from 2003 to 2018. The simulation of channel flow has been done using HEC RAS. It has been found that width/depth (w/d) ratio, bankfull channel width, channel area and sand-bed length of the river decrease due to groyne construction. On the other hand, channel maximum bankfull channel depth, depositional rate, the difference between average depth, maximum depth and braided index drastically increased. Increasing flow velocity, flow deflection with coarse and poorly sorted multimodal sediments near the tip of the groynes as well as decreasing flow velocity, curve flow path, accelerated sedimentation with elevating river bed have been observed. Altogether result indicates that the emerged groynes are effective in protecting the river banks at the cost of channel degradation.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Atmospheric nitrogen (N) deposition alters the priming effect (PE), which is defined as the change in native soil organic carbon (SOC) decomposition by exogenous C inputs. However, how the priming intensity varies under chemically heterogeneous N deposition, particularly with increasing labile C input, remains unclear.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We collected soils from a temperate forest in northeastern China that had received simulated organic and/or inorganic N deposition for 6 years. The soils were incubated with or without three levels of 〈sup〉13〈/sup〉C-labelled glucose solution for 152 days. CO〈sub〉2〈/sub〉 emission and its 〈sup〉13〈/sup〉C value were continuously measured to calculate the PE.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Enhanced SOC decomposition (i.e., a positive PE) was observed after glucose addition, regardless of the N deposition form. The PE intensity increased with the increase in the glucose addition level. However, organic N decreased the PE by 12.3-23.2%. The SOC-derived microbial biomass was 16.2-34.3% lower in organic N-treated soil, indicating that preferential utilization of exogenous labile C by microorganisms was responsible for the decrease in PE. The PE inhibition by organic N increased nonlinearly as a function of glucose level. Furthermore, the net annual change in SOC as a balance between the replenishment of added glucose-C and primed C was larger in organic N-treated soil due to a decrease in soil microbial metabolic quotient.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉In this study, we found that organic N deposition inhibited the PE, and the inhibition effect was intensified with increasing C inputs, favouring SOC sequestration.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Gatifloxacin (GAT), a new generation fluoroquinolone antibiotic, is widely used in the world but its environmental behavior has been rarely studied. In this study, the adsorption and desorption characteristics of GAT interacting with goethite and hematite at pH 6.0 ± 0.1 were investigated by batch experiments combining with attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. The results indicated that the total desorption ratio of GAT from goethite (70.8–84.8%) after three desorption steps was far higher than that from hematite (7.8–39.4%). In GAT adsorption onto goethite and hematite, a strong dependence on ionic strength was only observed for goethite. These all suggested that outer-sphere complexation was the main mechanism of GAT adsorption onto goethite while inner-sphere complexation dominated the adsorption onto hematite. No evidence for inner-sphere coordination was found at the goethite surface in this study under our conditions. Due to the difference in adsorption mechanisms, the pH at point of zero charge (pH〈sub〉pzc〈/sub〉) of hematite increased by about 1.0 unit after adsorption in relation to the pristine hematite, but the increase of goethite pH〈sub〉pzc〈/sub〉 was less than 0.3 unit. ATR-FTIR observations indicated that the surface hydration-shared ion pairs involving in strong hydrogen-bond interactions were predominant in GAT adsorption onto goethite. However, a bridging bidentate surface complex with hematite was most likely formed. Our results are helpful to advance understanding of the importance of comparatively strong ionic hydrogen-bonding interactions for adsorption processes of fluoroquinolones at the goethite/water interface. The findings of this study suggest that soils with goethite as the main mineral compositions may have very limited retention ability for GAT under field conditions.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Despite the high economic benefits of using an intensive agriculture system, the sustainable development of 〈em〉Lycium barbarum〈/em〉 L〈em〉.〈/em〉 cultivation in Northwest China is hindered by serious negative plant–soil feedback, which may be paralleled by the variation in the microbial rhizosphere community.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Here, we assessed the shift in the bacterial and fungal rhizosphere communities of 〈em〉L. barbarum〈/em〉 across a 20-year-old chronosequence of stands at two independent experimental sites in Ningxia, China via real-time PCR and high-throughput sequencing.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Stand age significantly influenced the α-diversity and abundance of the fungal rhizosphere community (〈em〉p〈/em〉 〈 0.05) but did not affect the bacterial community. Phytopathogenic fungi, including 〈em〉Alternaria〈/em〉, 〈em〉Fusarium〈/em〉, 〈em〉Gibberella〈/em〉, and 〈em〉Stemphylium〈/em〉, were progressively enriched in the rhizosphere as the plant aged. Structure equation model suggested that soil abiotic properties were the direct drivers for bacterial community, whereas stand age and edaphic factor coexplained the succession of the fungal rhizosphere community.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉Long-term monocropping decoupled plant–bacteria interaction and strengthened the colonization of fungal phytopathogens in 〈em〉L. barbarum〈/em〉 rhizosphere. This study presents novel assessments of the links between the shifts in microbial rhizosphere community and the negative plant–soil feedback of 〈em〉L. barbarum〈/em〉.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Intensive use of fertilizers in the agricultural lands and a swift-flying of coal and allied industries in Korba district, India in an unprecedented manner has led to groundwater contamination. Accordingly, an integrated modified DRASTIC and risk index model combined with other statistical techniques are applied to evaluate groundwater susceptibility and pollution risk in the region. The ArcGIS based spatial distribution map of the DRASTIC vulnerability index (DVI) reveals that the high (63%) and very high (23.61%) vulnerable zones identified in DVI map are reduced to that of 57.86% and 17.74%, respectively, when compared with pollution risk map. Results of sensitivity analysis, i.e., map removal sensitivity analysis and single parameter sensitivity analysis confirms that amongst seven DRASTIC parameters the net recharge parameter is the most influencing parameter in view of the groundwater contamination. The linear correlation coefficient (〈em〉r〈/em〉 = 0.89) obtained between risk index values and NO〈sub〉3〈/sub〉 concentrations alongside nearly 75% of the study area comprises of agricultural lands and forest/tree clad area corresponds to high to very high risk contamination zones reveal the model validation in the light of the influence of anthropogenic contamination factor in these zones. Furthermore, elevated iron concentration also supports the certain influence of geogenic contamination within the study area. In essence, this study can be effectively utilized in the planning and management of precious groundwater resources in high to very high vulnerable and risk zones of the study area, for its overall sustainable development.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Radium isotopes, 〈sup〉223〈/sup〉Ra and 〈sup〉224〈/sup〉Ra, and salinity measurements were used to assess submarine groundwater discharge from a karstic aquifer system into a coastal lagoon located at Oualidia (Morocco), on the Atlantic shoreline. The Oualidia lagoon is classified as a Ramsar area owing to its ecological significance. Groundwater discharge to the lagoon occurs as intertidal springs, submarine springs and probably diffuse leakage. Groundwater sources and discharge fluxes were determined using mass balance calculations following single and multi-box approaches. Calculated flow rates varied from 0.2 ± 0.2 to 1.2 ± 0.6 m〈sup〉3〈/sup〉 s〈sup〉−1〈/sup〉. The single-box model can be considered representative enough of water cycling and mixing within the Oualidia lagoon. However, a certain complexity of the hydrological processes should be taken into account as the discharge varies from low to high tide periods. The discharge amount suggests that the Oualidia lagoon may contribute significantly to the regional karstic discharge. Both the regional geological structure and the piezometric map indicate a convergence of groundwater to the area of the lagoon which is located in the bottom of a large geological depression. It is deduced that a close relationship exists between the karst development and the lagoon.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Sarchinar, Bestansur, and Tabin springs are large springs in Sulaymaniyah Governorate, Iraqi Kurdistan Region, which are considered for use as public supply. To evaluate the hydrochemical properties and heavy metals content of the selected springs, 80 water samples were collected continuously beginning on 24th Oct. 2015 through 24th July 2016. The results show that the water is odorless, colorless, contains a low value of total dissolved salts, predominant ions are calcium and bicarbonate, and all the water samples are not polluted with Cd〈sup〉2+〈/sup〉, Pb〈sup〉2+〈/sup〉, Cu〈sup〉2+〈/sup〉, Ni〈sup〉2+〈/sup〉 ions. The vulnerability of the selected springs is assessed using EPIK (epikarst, protective cover, infiltration condition, and karst network development) method. Vulnerability maps are counted as starting a new step for a better determination of springs’ water protection areas especially in the karst environment as the maps are generated based on different attributes as specified by adopted methods. Delineating springs’ protection zones are proposed depending on vulnerability assessment and recession curve analysis. Based on the EPIK vulnerability map, protection zones for all selected springs are defined using the equivalent relationship between the groundwater protection areas S and the protection factor 〈em〉F〈/em〉〈sub〉p〈/sub〉. Therefore, three protection areas for selected springs are recognized and based on recession curve analysis; two vulnerability scenarios are defined with an assessment of the proper dimensions of the protection zones for selected springs. The delineated protection zones around the tapped springs consist of three zones (immediate, inner, and outer protection zones).〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Twenty-four species of eucalypts were studied regarding their ability to grow under low P and their responsiveness to P inputs.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Growth and photosynthesis-related parameters were evaluated.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Growth of all species was influenced by low P availability. No significant correlation was found between leaf P concentration and biomass, indicating that P concentrations in leaves cannot be solely considered an indication of the responsiveness to P in eucalypts. Species responsive to P-input (high agronomic P efficiency values, APE) were those with low P use efficiency - PUE (here assessed as relative efficiency of P-use, REP) and low P uptake efficiency (PUpE). But, non-responsive species were related to higher P-efficiency under low soil P-availability. 〈em〉Eucalyptus tereticornis〈/em〉, 〈em〉E. cladocalyx〈/em〉, 〈em〉E. globulus〈/em〉 and 〈em〉E. camaldulensis〈/em〉 were efficient under low-P availability. Whereas, 〈em〉E. crebra〈/em〉 and 〈em〉E acmenoides〈/em〉 were the most responsive species, with high APE, suggesting that for these species P-inputs are needed to guarantee plant growth. The root:shoot ratio remained constant at different P availabilities, suggesting that biomass allocation towards the root in response to P and greater investment in roots were not correlated with greater PUE. Under limited P, 〈em〉E. robusta〈/em〉 and 〈em〉E. botryoides〈/em〉 exhibited low foliar P contents and higher root:shoot ratios than those of other species with higher P contents, indicating that greater root investment does not necessarily result in greater PUE.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉The results suggest that the divergence among species is probably related to different mechanisms, which may improve P-use efficiency.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Excessive boron (B) can pose toxicity to many plant species, and consequently restricts land utilization in B-laden regions. The purpose of this study was to identify “agretti” (〈em〉Salsola soda〈/em〉) as an alternative B-tolerant food crop.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Both pot and hydroponic experiments were conducted for measuring biomass, total phenolic content and B absorption of 〈em〉S. soda〈/em〉 exposed to varied B treatments. Mineral element accumulation in 〈em〉S. soda〈/em〉 growing in pots was also determined.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉No typical B toxicity symptoms were observed in pot experiment, and only slight B toxicity symptoms were observed in hydroponically-grown plants at 50, 100 and 200 mg B L〈sup〉−1〈/sup〉 treatments. Biomass production was not affected in either experiment. The response of total phenolic content to B exposure varied with growing medium, parts of tissues, B treatments, and exposure times. Boron predominantly accumulated in leaves and increased with increasing B treatments in both experiments. Increased exposure time increased the transport of B from root to shoot. Increasing B treatment generally reduced the accumulation of phosphorus, manganese, selenium and arsenic, but increased the accumulation of B, molybdenum and cadmium in 〈em〉S. soda〈/em〉 under specific B treatments, even the accumulation of such elements was still safe for human consumption.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉〈em〉S. soda〈/em〉 appears to be a promising alternative crop to grow in B-laden regions such as the western SJV of Central California.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The availability of safe drinking water is imperative for healthy life but access to safe drinking water has become a major problem around the world, especially in developing countries such as Pakistan. The present study aimed to: assess the suitability of groundwater for potable use in Gujranwala district, Pakistan; examine the spatial distribution patterns of water quality parameters; and to identify the prevalence of waterborne diseases among locals and health risks to humans due to the consumption of groundwater. Eighty drinking water samples were collected from different areas of Gujranwala and analyzed following Standard American Public Health Association methods. A questionnaire survey related to the incidence of waterborne diseases in the area was also carried out. The averaged water quality index (AWQI) was computed using ArcGIS 10.3 model builder. The AWQI map indicated that the water quality was generally suitable for potable use with regard to its physiochemical parameters. However, 97.5% water samples were found to be bacteriologically contaminated. The mean concentration of metals in drinking water showed a decreasing trend in the order Cr 〉 Cu 〉 Zn 〉 As 〉 Co 〉 Ni 〉 Cd. Health risk index (HRI) was also calculated for exposure to metal concentration. For Cd, one sample showed an HRI value 〉 1 i.e. 1.59 and 1.36 for child and adult, respectively, while HRI for As showed values 〉 1 varying between 0.5 and 11 for child, and 0.4 and 9.6 for adults. For other metals HRIs was less than 1 (considered to be safe for the consumers). The study concludes that groundwater quality of study areas of Gujranwala has mostly deteriorated.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Rhizosheath is known as a layer of adhering soil particle to the root surface. Despite several speculations, the positive function of rhizosheath in acquisition of water and nutrients from drying soil has not yet been experimentally proven. The objective of this study was to experimentally show whether an enhanced rhizosheath formation could help plants to better access water from drying soil.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Eight wheat cultivars were grown in a sandy-loam soil. When plants were 35 days old let dry soil to a water content at which evident wilting symptoms appeared on the plant leaves. During this drying cycle, soil water content and transpiration rate of plants were gravimetrically measured by weighing the plant pots. At the end of this drying cycle, the roots were excavated out of the soil and the rhizosheath formation was gravimetrically quantified by weighing the soil attached to the root system.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The results showed that plant cultivars with greater rhizosheath formation could sustain higher transpiration rates at dry condition (water content of 0.07 cm〈sup〉3〈/sup〉 cm〈sup〉−3〈/sup〉) while the plant cultivars with lower rhizosheath formation suffered from drought stress and reached their permanent wilting points at the same water content.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉The findings of this study gathered evidence that under severe drought condition plant cultivars with an enhanced rhizosheath formation could better survive by sustaining their transpirational and nutritional demands.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background〈/h3〉 〈p〉Agroforestry systems have enhanced diversity of cultivated plants compared to monocultures, and are expected to affect associated biodiversity. Despite a growing body of literature on the importance of soil fauna, the known effects of different agroforestry types on soil fauna communities and functions have not yet been synthesized.〈/p〉 〈/span〉 〈span〉 〈h3〉Scope〈/h3〉 〈p〉We scanned publications on soil fauna in agroforestry systems. Our aim was to give an overview of strengths and weaknesses of the existing data, in terms of spatial coverage and representation of diverse agroforestry types and soil fauna groups and functions.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Our database includes sixty-seven articles, mostly focusing on tropical regions and perennial crop agroforestry systems. Soil macrofauna are the most studied fauna group. The most common question addressed is the comparison of the effect of land use types on communities. Effects on fauna abundance and diversity are mainly positive when agroforestry is compared to cropland, and neutral or negative when compared to forests. Few publications actually measure soil fauna functions, or characterize their interactions and evolution in time and space depending on system design and management. Further work on soil fauna in agroforestry should harness ecological theory and address questions of spatial structure and scale, temporal dynamics and ecological interaction networks and how they determine ecosystem functioning.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Rising atmospheric CO〈sub〉2〈/sub〉 causes an increase in dissolved inorganic carbon (DIC) availability in aquatic ecosystems, further affecting plant growth and aquatic ecosystem production. Belowground carbon input can strongly influence microbial processes (carbon or nitrogen cycling). However, changes in sediment microbial abundance and community structure have not been thoroughly assessed in freshwater ecosystems under rising atmospheric CO〈sub〉2〈/sub〉. A pot experiment was conducted using fragments of the submerged macrophyte 〈em〉Myriophyllum spicatum〈/em〉 L. to study plant and sediment microbial responses to increasing aquatic carbon availability under rising atmospheric CO〈sub〉2〈/sub〉. Three DIC levels of overlying water were set up by continuous bubbling with different concentrations of CO〈sub〉2〈/sub〉. Higher biomass accumulation, chlorophyll content, nitrate reductase activity, and leaf N content were observed in 〈em〉M. spicatum〈/em〉 under high DIC level in CO〈sub〉2〈/sub〉 treatment. The increased DIC level in CO〈sub〉2〈/sub〉 treatment reduced sediment ammonium nitrogen, dissolved organic nitrogen content, microbial biomass carbon content and phenol oxidase activity, but not microbial biomass nitrogen content, urease and N-acetyl-β-D-glucosaminidase activities. 〈em〉NifH〈/em〉 abundance significantly decreased with the increasing DIC levels in CO〈sub〉2〈/sub〉 treatment, while bacterial 16S rRNA abundance was not affected. Redundancy analysis results indicated a modest but obvious shift in sediment microbial community compositions among different levels of DIC. Variation partitioning revealed a strong interaction between plant traits and sediment properties and their regulation of sediment microbial compositions at different DIC levels. It seemed that an intensified competition for essential resources between 〈em〉M. spicatum〈/em〉 and sediment microbes occurred under increased aquatic DIC availability caused by rising atmospheric CO〈sub〉2〈/sub〉.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Aim to unveil the functions of VcLon1 in plant Fe use efficiency.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉RT-PCR was used to analyze the expression profile of 〈em〉VcLon1〈/em〉. 〈em〉VcLon1〈/em〉 was expressed in 〈em〉Nicotiana benthamiana〈/em〉, and its homologous tobacco gene was silenced using RNAi. The differences in biomass growth, oxidative stress and chloroplast ultrastructure between wild type and transgenic 〈em〉Nicotiana benthaminana〈/em〉 were analyzed after being subjected to Fe deficiency stress〈em〉.〈/em〉〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The RT-PCR showed that the expression of 〈em〉VcLon1〈/em〉 was significantly higher in young leaves than in old leaves or in leaves treated with Fe deficiency stress, indicating that VcLon1 is involved in reactive oxygen species (ROS) homeostasis induced by senescence or Fe deficiency. Compared with wild-type, overexpression of 〈em〉VcLon1〈/em〉 in 〈em〉Nicotiana benthamiana〈/em〉 reduced damage to chloroplast structure induced by Fe deficiency. Furthermore, the contents of H〈sub〉2〈/sub〉O〈sub〉2〈/sub〉, MDA and carbonylated protein in leaves were kept at a low level, and antioxidant enzyme activities in chloroplasts such as SOD and APX are also generally higher in chloroplasts with 〈em〉VcLon1〈/em〉 overexpression. In contrast, the oxidative stress levels in 〈em〉NbLon1〈/em〉 RNAi silenced 〈em〉Nicotiana benthamiana〈/em〉 leaves showed opposite trends.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Under Fe deficiency stress, VcLon1 reduces oxidative damage in plants by degrading carbonylated proteins in organelles such as chloroplasts and effectively maintains the structure and function of organelles and the activity of functional proteins, contributing to Fe use efficiency in plants.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aim〈/h3〉 〈p〉Plant-endophytic associations exist only when equilibrium is maintained between both partners. This study analyses the properties of endophytic fungi inhabiting a halophyte growing in high soil salinity and tests whether these fungi are beneficial or detrimental when non-host plants are inoculated.〈/p〉 〈/span〉 〈span〉 〈h3〉Method〈/h3〉 〈p〉Fungi were isolated from 〈em〉Salicornia europaea〈/em〉 collected from two sites differing in salinization history (anthropogenic and naturally saline) and analyzed for plant growth promoting abilities and non-host plant interactions.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Most isolated fungi belonged to Ascomycota (96%) including dematiaceous fungi and commonly known plant pathogens and saprobes. The strains were metabolically active for siderophores, polyamines and indole-3-acetic acid (mainly 〈em〉Aureobasidium〈/em〉 sp.) with very low activity for phosphatases. Many showed proteolytic, lipolytic, chitinolytic, cellulolytic and amylolytic activities but low pectolytic activity. Different activities between similar fungal species found in both sites were particularly seen for 〈em〉Epiccocum〈/em〉 sp., 〈em〉Arthrinium〈/em〉 sp. and 〈em〉Trichoderma〈/em〉 sp. Inoculating the non-host 〈em〉Lolium perenne〈/em〉 with selected fungi increased plant growth, mainly in the symbiont (〈em〉Epichloë〈/em〉)-free variety. 〈em〉Arthrinium gamsii〈/em〉 CR1-9 and 〈em〉Stereum gausapatum〈/em〉 ISK3-11 were most effective for plant growth promotion.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉This research suggests that host lifestyle and soil characteristics have a strong effect on endophytic fungi, and environmental stress could disturb the plant-fungi relations. In favourable conditions, these fungi may be effective in facilitating crop production in non-cultivable saline lands.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉To optimize assay conditions of two common methods for measuring potential free-living nitrogen-fixation (FLNF), acetylene reduction assay (ARA) and 〈sup〉15〈/sup〉N〈sub〉2〈/sub〉-incorporation (〈sup〉15〈/sup〉N〈sub〉2〈/sub〉), for use with soil/rhizosphere samples.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We tested the impact of different carbon (C) sources, oxygen concentrations (O〈sub〉2〈/sub〉), and incubation times on FLNF rates of two low-fertility Michigan soils via ARA and 〈sup〉15〈/sup〉N〈sub〉2〈/sub〉.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉FLNF rates were greatest with addition of a C cocktail, at low O〈sub〉2〈/sub〉, and with 7-day incubations for both methods. FLNF via ARA was 1700x greater with a C cocktail versus glucose only and via 〈sup〉15〈/sup〉N〈sub〉2〈/sub〉 was 17x greater with a C cocktail compared to other C sources and no-C controls. Specific O〈sub〉2〈/sub〉 optimum varied by method and site. A 7-day incubation was needed for the ARA, but a 3-day incubation was suitable for 〈sup〉15〈/sup〉N〈sub〉2〈/sub〉. Lastly, we confirm previously identified issues with the ARA of acetylene-independent ethylene production/consumption resulting in potential FLNF measurement error of 1.3–52.3 μg N g〈sup〉−1〈/sup〉 day〈sup〉−1〈/sup〉.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉We present an optimized method for measuring potential FLNF in soil/rhizosphere samples which will allow for consistent and comparable FLNF rate measurements. Researchers should account for C source, O〈sub〉2〈/sub〉, and incubation time when assessing FLNF and use the ARA method with caution.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Groundwater quality assessment is important for potability and for industrial and agricultural uses of water. Groundwater quality is a measure of contamination by chemicals, biological organisms, sediments, and heat. Reductions of groundwater quality in some areas due to high rates of consumption have prompted the identification of regions in which extraction will be focused. In this study, geographic information systems (GIS)-based analytical network process (ANP) and analytical hierarchical process (AHP) multiple-criteria decision-making techniques using a fuzzy-quantifier algorithm were devised to model groundwater quality in northern Fars Province, Iran. Groundwater quality was assessed by measuring calcium (Ca), chlorine (Cl), magnesium (Mg), thorium (Th), sodium (Na), sulfate (SO〈sub〉4〈/sub〉), electrical conductivity (EC), and total dissolved solids (TDS). A membership function based on World Health Organization (WHO) groundwater quality standards was used to create a fuzzy map of each parameter in ArcGIS. Fuzzy maps were generated for each layer using a trapezoidal membership function. The AHP and ANP methods provided weights for each layer to generate groundwater quality maps. Using a feature-selection algorithm, the relative importance of the factors that affect groundwater quality was determined. The results show that, according to the AUC values, ANP generates higher accuracy than fuzzy-AHP (0.954 in comparison to 0.845). The feature-selection algorithm indicates that Ca, Cl, EC, and Na had the greatest impact on groundwater quality conditions. Using ANP and selecting the most important factors can be an economical way, in terms of time and money, to produce highly accurate information that can be used to predict local groundwater quality.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Backgrounds〈/h3〉 〈p〉There are growing concerns regarding the restoration of karst rocky desertification (KRD) areas. However, the soil conditions and its residing microorganisms, which are essential for the plants, remain largely unclear.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We studied soil characteristics and microbial communities in natural forests (non-KRD) and shrubs with eroded soil and surface soil run-off, using Illumina Miseq sequencing.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Our results showed that despite KRD reduced soil fertility and altered microbial community structures, microbial diversity did not diminish. Interestingly, bacterial OTU richness and diversity were greater in the KRD areas than in the non-KRD areas, which had relatively greater plant density and diversity. Fungal OTU richness and diversity remained unchanged by KRD. Although the KRD areas had been clear-cut and trees were mostly absent, ectomycorrhizal fungi did not differ in diversity and relative abundance between the two land types, indicating that the KRD shrubs hosted surprisingly diverse and abundant ectomycorrhizal fungi.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Our results highlight the highly diverse microbes under environmental and anthropogenic stresses in KRD areas. Despite the fact that degraded soil properties and an altered microbial community structure remain, KRD did not erode ectomycorrhizal fungal species richness, which is crucial in the revegetation of trees in KRD areas.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The stability of natural and man-made rock slopes has always been of great concern for engineering geology studies. To ensure a safe and functional design of excavated slopes, the first and most crucial step is to use reliable input parameters while searching for possible failure modes at the preliminary design stage. It is highly important for practicing engineers to determine the best material property for a chosen failure criterion. This study aims to analyze the stability condition of a case road cut from different aspects. For this purpose, two well-known failure criteria proposed for rock masses and rock joints were applied in the study depending on the scale of the problem in the slope face. The Hoek–Brown failure criterion was used to obtain input data required for modeling intact rock and rock mass behavior, and the Barton-Bandis shear strength criterion to determine the input data necessary for the modeling of rock joint behavior. 〈em〉SMRTool〈/em〉 and 〈em〉Dips 7.0〈/em〉 programs were utilized to analyze the structurally controlled instabilities due to discontinuities in the cut slope. 〈em〉Slide 2018〈/em〉 and 〈em〉RS2 9.0〈/em〉 programs were executed to examine the stability condition of cut slope subjected to circular and non-circular failures, respectively. According to the results of numerical and limit equilibrium methods, no stability problem occurred in the case of slope due to non-sliding circular or non-circular slip failures. However, according to the results of empirical and kinematical analyses, some structurally controlled sliding failure modes of planar, wedge, and toppling were expected on the slope face. The result of this study clearly shows that to reach a final decision, different analysis methods should be used to evaluate the stability of cut slopes by considering various conditions of jointed rock masses.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Initial substrate chemical characteristics are the most important factor in the regulation of fine root decomposition. However, it remains unclear how nitrogen (N) deposition changes the decomposition process by affecting initial substrate chemical characteristics with different fine root diameter sizes.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We compared the root decomposition processes across three diameter sizes (very fine roots, 〈 0.5 mm; intermediate fine roots, 0.5–1.0 mm; largest fine roots, 1.0–2.0 mm) of 〈em〉Pinus tabulaeformis〈/em〉 treated with N addition (control, low, medium, high N are 0, 3, 6, and 9 g N m〈sup〉−2〈/sup〉 y〈sup〉−1〈/sup〉 respectively) for two years.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉(1) The root decomposition rates, which were mainly determined by initial N, phosphorus (P), cellulose and lignin concentrations, and carbon (C)/N and lignin/N ratios, increased with the root diameters. (2) The effect of N addition on fine root decomposition rate was not significant (〈em〉P〈/em〉 〉 0.05), but low N addition enhanced the correlation coefficients between initial chemical indexes and decomposition rates. (3) Low N addition increased the release rates of C and cellulose in the very fine roots but not intermediate fine and largest fine roots, while the medium and high N addition decreased the release rates of N, P, cellulose and lignin in the very fine and intermediate fine roots by affecting the initial C, N, P, starch, cellulose and lignin concentrations. (4) Release of compounds from large diameter fine roots is less responsive to N addition than that from the small ones.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉The initial substrate chemistry plays an important role during the N addition affecting fine root decomposition and release of chemical compounds. Our results suggest that N deposition may change the biogeochemical processes of forest ecosystems by affecting the release of compounds from fine roots.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Root niche partitioning among trees/shrubs and grasses facilitates their coexistence in savannas, but little is known regarding root distribution patterns of co-occurring woody plants, and how they might differ on contrasting soils.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We quantified root distributions of co-occurring shrubs to 2 m on argillic and non-argillic soils.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Root biomass in the two shrub communities was 3- to 5- fold greater than that in the grassland community. 〈em〉Prosopis glandulosa〈/em〉, the dominant overstory species was deep-rooted, while the dominant understory shrub, 〈em〉Zanthoxylum fagara〈/em〉, was shallow-rooted (47% vs. 25% of root density at depths 〉0.4 m). Shrubs on argillic soils had less aboveground and greater belowground mass than those on non-argillic soils. Root biomass and density on argillic soils was elevated at shallow (〈 0.4 m) depths, whereas root density of the same species on non-argillic soils were skewed to depths 〉0.4 m. Root density decreased exponentially with increasing distance from woody patch perimeters.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Belowground biomass (carbon) pools increased markedly with grassland-to-shrubland state change. The presence/absence of a restrictive barrier had substantial effects on root distributions and above- vs. belowground biomass allocation. Differences in root distribution patterns of co-occurring woody species would facilitate their co-existence.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Riverine organic carbon export plays a major role in the global carbon cycle. The biodegradability of riverine dissolved organic carbon (DOC) determines both greenhouse gas emissions and the amount of refractory DOC. However, seasonal changes in organic carbon and factors controlling DOC biodegradation are still not well understood in large rivers. Here, we examined the monthly changes in concentrations of particulate organic carbon (POC) and DOC in a large subtropical river (Changjiang River, Nantong Section, China) in 2017. We also measured biodegradable DOC (BDOC), specific UV absorbance (SUVA〈sub〉254〈/sub〉), neutral sugar concentrations and their compositions. The results showed that POC had no significant relationship with water temperature, precipitation, and discharge. DOC concentrations were significantly negatively correlated with all these parameters, and water temperature explained 61% of the variance in DOC. BDOC concentrations varied between 0 and 14.01 ± 1.22 mg C/L and were closely associated with DOC (〈em〉R〈/em〉〈sup〉2〈/sup〉 = 0.85, 〈em〉p〈/em〉 〈 0.001). The negative correlation between BDOC% and SUVA〈sub〉254〈/sub〉 (〈em〉R〈/em〉〈sup〉2〈/sup〉 = 0.63, 〈em〉p〈/em〉 〈 0.01) suggested that high aromaticity was associated with low bioavailability. Water temperature was positively correlated with total neutral sugar yield (〈em〉R〈/em〉〈sup〉2〈/sup〉 = 0.43, 〈em〉p〈/em〉 〈 0.001), which was mainly composed of glucose and deoxysugars in the warm season; thus, neutral sugar composition is a useful indicator of DOC decomposition. Taken together, our study showed that POC has no clear seasonal change, while DOC exhibits a strong seasonal change, and high water temperature leads to low BDOC in a large subtropical river.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Soft computing methods are increasingly being used as substitutes for traditional statistical analysis tools to predict uniaxial compressive strength (UCS) and elastic modulus (〈em〉E〈/em〉). In this paper, multiple regression (MR) analysis and fuzzy inference system (FIS) were used to estimate UCS and 〈em〉E〈/em〉 of migmatites in the Sanandaj–Sirjan zone in Iran. A series of rock index properties including porosity (〈em〉n〈/em〉), cylinderical punch index (CPI), block punch index (BPI), Brazilian tensile strength (BTS), point load index (IS〈sub〉(50)〈/sub〉), and P-wave velocity (〈em〉V〈/em〉〈sub〉P〈/sub〉) were measured and employed to develop six prediction models. Amongst the rock indices, IS〈sub〉(50)〈/sub〉 and 〈em〉V〈/em〉〈sub〉p〈/sub〉 had the best correlations with UCS and 〈em〉E〈/em〉, respectively. Performance evaluation indicated that FIS is a more reliable tool for predicting UCS and 〈em〉E〈/em〉 of migmatites, due to its ability in managing uncertainties in the test results with clarity and precision. The results indicated that FIS model 2 which employs 〈em〉n〈/em〉, CPI, BTS, and 〈em〉V〈/em〉〈sub〉P〈/sub〉 as input variables and FIS model 3 which employs 〈em〉n〈/em〉, CPI, IS〈sub〉(50)〈/sub〉, and 〈em〉V〈/em〉〈sub〉P〈/sub〉, performed better than other models in predicting UCS, while, FIS model 2 also produced the best results for predicting 〈em〉E〈/em〉. The results of performance evaluation demonstrate that the developed models are highly efficient for prediction and the input parameters have notable influence on UCS and 〈em〉E〈/em〉.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉In this study, we investigated the effects of reduced snow depth on plant phenology, productivity, nitrogen (N) cycling, and N use in canopy and understory vegetation. We hypothesized that decreased snow depth would hasten the timing of leaf flushing and N uptake in understory vegetation, increasing its N competitive advantage over canopy trees.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Snow removal did not directly affect the phenology of either canopy or understory vegetation. Understory vegetation took up more N in the snow removal plots than in the control plots, particularly in the mid- to late-growing season. Leaf production and N uptake in canopy trees also did not differ between the control and snow removal plots, but N resorption efficiency in the snow removal plots (57.6%) was significantly higher than those in control plots (50.0%).〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Increased N uptake by understory plants may induce N limitation in canopy trees, which in turn may cause canopy trees to increase their N use efficiency. Such competitive advantage of understory vegetation over canopy trees against snow reduction may affect N cycling via litter quality and quantity not only just after the growing season but also in subsequent seasons.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Whilst several studies have shown that edaphic variability influences species composition in nutrient-poor tropical forests, the determinants of local species distributions and, in particular, how these change from younger to mature individuals in such forests are still under debate, and have been poorly explored in tropical heath forests that are among the least fertile tropical forest ecosystems.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We investigated the influence of soil fertility and topography on a Bornean heath forest species composition, α-, β-diversity and tree size structure among size classes by recording all trees ≥1 cm DBH in 16 forest plots totalling 0.36 ha.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Tree species distributions generally followed gradients in available Al and soil depth; α- and β-diversity were linked to soil depth, and to some extent also to pH and the H:Al ratio. In contrast, forest structural attributes (basal area and stem density) were negatively correlated with both available and total P and a wider suite of soil nutrients, although trees ≥10 cm DBH were positively correlated with total P.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉Our study shows that heath forest species distribution, richness and structure is related to both edaphic and topographic characteristics and that soil acidity might have a strong influence in shaping these forests’ features. Among size classes, small trees are less influenced by soil and topography, whereas the sensitivity to these variables increases with tree size. We thus highlight that multiple edaphic factors influence different aspects of tropical forest structure, including different tree life stages, and species composition.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉The trend of soil degradation in intensive open coffee systems is well-documented. This study highlights the impact of young shade trees on soil quality only 4 years after their intercropping with coffee.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉18 young shade trees belonging to three tree species (〈em〉Cinnamomum camphora〈/em〉, 〈em〉Bishofia javanica〈/em〉 and 〈em〉Jacaranda mimosifolia〈/em〉) were selected in an intensive coffee system in Southern Yunnan. Soil samples (0–20 cm) were tested for chemical composition, soil communities and soil enzyme activities under their canopies and in open areas, both in coffee rows and inter-rows, once during the rainy and once during the dry season. Additionally, root systems were characterized using trenches. Soil water profiles and litterfall were monitored along the production cycle. Coffee yield was recorded for two consecutive years.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉We detected a positive impact of all shade tree species on soil chemical, biological and biochemical components, especially during the dry season. This positive impact included higher soil organic matter (+10%) and more abundant soil microbial communities (+64%) under shaded coffee than under open coffee. Furthermore, shaded coffee trees yielded as much as open coffee trees, except under 〈em〉C. camphora〈/em〉, probably due to high below-ground competition.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉These results demonstrate that carefully selected shade trees can rapidly contribute to preserving and/or restoring soil quality in intensive coffee systems, while maintaining high coffee yield.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Traits of the plant root system architecture (RSA) play a key role in crop performance. Therefore, architectural root traits are becoming increasingly important in plant phenotyping. In this study, we use a mathematical model to investigate the sensitivity of characteristic root system measures, obtained from different classical field root sampling schemes, to RSA parameters.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Root systems of wheat and maize were simulated and sampled virtually to mimic real field experiments using the root system architecture (RSA) model CRootBox. By means of a sensitivity analysis, we found RSA parameters that significantly influenced the virtual field sampling results. To identify correlations between sensitivities, we carried out a principal component analysis.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉We found that the parameters of zero order roots are the most sensitive, and parameters of higher order roots are less sensitive. Moreover, different characteristic root system measures showed different sensitivity to RSA parameters. RSA parameters that could be derived independently from different types of field observations were identified.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Selection of characteristic root system measures and parameters is essential to reduce the problem of parameter equifinality in inverse modeling with multi-parameter models and is an important step in the characterization of root traits from field observations.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Moso bamboo (〈em〉Phyllostachys edulis〈/em〉) invasions into adjacent forests are becoming increasingly common. Moso bamboo invasions affect litter quality, soil nutrients, and microbial community composition. Although these effects likely vary among invaded sites and forest types, this has not been investigated.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We investigated moso bamboo invasion effects on carbon (C) and other major nutrients of litter and soil, as well as soil microbial community composition determined by phospholipid fatty acids (PLFAs) in broadleaf or coniferous forests at three different sites in China.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Ordinations indicated that the effects of invasions on soil nutrients, litter nutrients, and soil microbial composition each varied among forest types and sites. Invasions consistently decreased litter C. Invasions tended to have larger effects on soil nutrients in coniferous forests. Except for bacterial groups in one coniferous forest site, invasions had positive effects on every soil group.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Variations in direction and magnitude of invasion effects on litter properties, soil properties, and soil communities among community types and sites suggest that studies of effects of invasions on soils in a single invaded community may not be able to predict effects of an invasion at other locations, even when the original community is similar or occurs in the same site.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Mine tailings are challenging substrates for ecological restoration, as the establishment of diverse native plant communities can be constrained by a range of edaphic factors. Thus, the ability to restore native vegetation communities will depend upon developing a clear evidence-base as to what types of species and communities are likely sustainably reinstated on such altered substrates. As global tailings production and the cumulative footprint of tailings storage facilities continue to grow, understanding the effect of edaphic filters on community establishment is foundational for developing effective restoration solutions for tailings.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We standardised growth rate estimates derived from nine root and shoot parameters for plants grown in magnetite tailings and natural topsoil, using crops (eight species) to characterise previously identified plant responses and native plants (40 species) to understand the impact of edaphic conditions on the species pool available for restoration.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The edaphic conditions of unweathered magnetite tailings select against the majority of native plant species and nutrient-acquisition guilds (approximately 75% of reference floristic biodiversity), with plant development on tailings compared with natural topsoil compromised in a number of variables in all but six species. Plant growth on tailings was limited by a lack of available nitrogen (N) and high alkalinity (pH 〉9), and seedling growth and development was positively associated with seed N concentration. Calcicole species and species from N〈sub〉2〈/sub〉-fixing and cluster root-producing strategies performed better on tailings than calcifuge species and species without specialised nutrient-acquisition strategy or those reliant upon mycorrhizal associations.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉The return of plant communities native to highly weathered, acidic soils on magnetite tailings is likely unsuccessful, unless strategies to ameliorate substrate hostility through acidification of the soil profile and improving N availability are prioritised.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉The low phosphorus (P) fertilizer use efficiency in weathered, P deficient soils calls for better fertilizer formulations. We previously formulated nanoparticles containing P (NP-P) that were a successful fertilizer in nutrient solution. This study was set up to test the fate and the bioavailability of nanofertilizer-P and of that of native (colloid) P naturally present in soil.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉The NP-P consisted of nano-ferrihydrite (~ 10 nm) loaded with phosphate (P-〈em〉n〈/em〉Fh) and stabilized with either natural organic matter (NOM) or hexametaphosphate (HMP). Natural colloid concentrations were increased with KOH addition, as deflocculating agent, to soil; all tests used samples from P deficient, highly weathered soils.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Pot trials with rice seedlings did not reveal larger P uptake in the NP-P amended soils compared to equal doses of soluble PO〈sub〉4〈/sub〉 or soluble HMP. Total Fe concentrations in soil solutions were unaffected by NP-P addition, whereas natural colloidal Fe and P markedly increased by KOH addition. The bioavailability of native colloidal P, mobilized by KOH addition, could not be assessed due to lack of growth, likely related to collapse of the soil structure.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉This study showed that P-loaded iron oxyhydroxide NPs insufficiently enhanced soluble P in soil to offer benefits over soluble fertilizers, likely because of a combined effect of lower diffusivity of NPs compared to P〈sub〉i〈/sub〉 and lower bioavailability of NP-P than P〈sub〉i〈/sub〉. Smaller particles or small labile organic colloids might offer an improvement in both aspects.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉A surface drainage system (SDS) controls catchment hydrology and acts as an indicator of geomorphologic processes. In this study, a field-based and GIS-integrated approach enabling reconstruction of a surface drainage system, which operates during heavy rainfall in small flysch catchments, has been proposed. The reconstruction is based on the ALS-LIDAR data. The reconstruction of the SDS gave the opportunity for analysis of the changes between the river system and the SDS operating during heavy rainfalls. Results have revealed that the SDS operating during heavy rainfalls is several times better developed than the river system. The density has increased from c.a. 1.5 to 13.7 km·km〈sup〉− 2〈/sup〉. Moreover, the structure of the SDS has changed, what was confirmed by the parameters of the Hortonian type of the analyses. The most significant changes were related to the first- and second-order streams. These streams were, the most frequently, the man-origin incisions and natural-origin incisions/concavities on the hillslopes conditioned by micro-relief. The man-origin sub-system reached up to 35% of the SDS functioning during heavy rainfalls, whereas the sub-system composed of incisions/concavities conditioned by micro-relief reached up to 24% of this SDS. Smaller lateral valleys included to the SDS during heavy rainfalls constitute up to 37% of the SDS. The permanent streams constitute the remaining part of the SDS. Changes in the SDS have the influence on the drainage pattern, hydrological response of a catchment, and intensity of geomorphological processes; therefore, the changes in the SDS and their consequences have been discussed.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The Mediterranean mountains of Morocco (“Rif”) show an intense sensitivity to hydro-climatic hazards. Hydrologic achievements are thwarted by a series of constraints that request strategic consolidation. The acceleration of socioeconomic activities is causing an increasing pressure on water resources that are already facing insufficiency and degradation problems. This paper aims at geophysical characterization of the most important intramountainous alluvial aquifer on the Rif, Ghis–Nekor. Its electrical and hydrogeological properties are investigated through a correlation of 2D electrical resistivity tomography (ERT), vertical electrical sounding (VES) and borehole data. The results have shown that this monolayer coastal aquifer is constituted of large coarse sand, gravel and pebble deposits that overlay substrata of different hydrogeological and electrical types. A geoenvironmental survey was also carried out in the coastal zone and near the uncontrolled waste landfill of Beni Bouayach. The 2D ERT profiles indicate an increase of marine water intrusion (over 2 km) particularly on the eastern bank of the Nekor river. They also show a spread of the landfill leachate flow path beyond 1 km in the direction of the groundwater flow (to the North), towards an area of hydrogeological interest located east of the city of Imzouren. These results imply a socioeconomic and environmental vulnerability that evokes an immediate intervention to restore weakened equilibrium.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Water temperature impacts many processes in rivers, and it is determined by various environmental factors. This study proposed an extreme learning machine (ELM)-based model to predict daily water temperature for rivers. Air temperature (〈em〉T〈/em〉〈sub〉a〈/sub〉), discharge (〈em〉Q〈/em〉) and the day of the year (DOY) were used as predictors. Three rivers characterized by different hydrological conditions were investigated to test the modeling performances and the model results were compared with multilayer perceptron neural network (MLPNN) and simple multiple linear regression (MLR) models. Results showed that inclusion of three inputs as predictors (〈em〉T〈/em〉〈sub〉a〈/sub〉, 〈em〉Q〈/em〉 and the DOY) yielded the best modeling accuracy for all the developed models. It was also found that 〈em〉Q〈/em〉 played a minor role and 〈em〉T〈/em〉〈sub〉a〈/sub〉 and DOY are the most important explanatory variables for river water temperature predictions. Additionally, sigmoidal and radial basis activation functions within the ELM model performed the best for river water temperature forecasting. ELM and MLPNN models outperformed MLR model, and ELM model with sigmoidal and radial basis activation functions performed comparably to MLPNN model. Overall, results indicated that the ELM model developed in this study can be effectively used for river water temperature predictions.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Wetlands play vital roles as sinks for metal contaminants. Some wetland plants accumulate manganese (Mn) oxides in the black biofilm around roots and rhizomes, although the underlying mechanism is still unclear. Our aim is to determine the role of endophytic bacteria in the formation of Mn deposits in the wetland plant 〈em〉Suaeda salsa〈/em〉 Pall. as well as the underlying chemical and molecular mechanisms.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Manganese-oxidizing endophytic bacteria were isolated with leucoberbeline blue (LBB) and further identified via the phylogenetic analysis. The Mn content and black deposit characteristics of laboratory-cultivated plants before/after co-cultivation of bacteria were investigated by inductively-coupled plasma optical emission spectrometry (ICP-OES), a scanning electron microscope equipped with an energy energy-dispersive X-ray spectroscopye (SEM-EDX), and X-ray fluorescence (XRF). The chemical structures of the biogenic Mn minerals were characterized via spectra of X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), and selected area electron diffraction (SAED). Proteomic analyses, coupled with the enzymic assays were performed to identify the enzymes involved in the Mn oxidation.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉We observed black deposits containing Mn oxides in the belowground and aboveground tissues of 〈em〉S. salsa〈/em〉. Three Mn-tolerant bacterial strains were isolated from the plants, and two of them possessed Mn(II) oxidation capacities, which were identified as 〈em〉Pantoea eucrina〈/em〉 SS01 and 〈em〉Pseudomonas composti〈/em〉 SS02. Co-cultivation of the two isolates with 〈em〉S. salsa〈/em〉 showed promoted plant growth and facilitated the formation of black precipitations on roots. Further results showed the different chemical compositions and cellular localizations of biogenic Mn oxides from the two strains. Hydrogen peroxide-detoxifying enzymes were involved in Mn oxidation, most likely mitigating oxidative stresses.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉We suggest a role of endophytic bacteria in Mn uptake and accumulation in the wetland plant 〈em〉S. salsa〈/em〉; our study thereby contributes to a better understanding of the plant-endophyte symbiosis in biogeochemical Mn cycling and wetland soil phytoremediation.〈/p〉 〈/span〉

Beschreibung: 〈p〉In the original publication the title of the article was published wrongly as “Geotechnical aspects and associated problems of Al-Shuaiba Lagoon soil, Red Sea c, Saudi Arabia”.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Northern peatlands store large amounts of soil organic carbon (C) that can be very sensitive to ongoing global warming. Recently it has been shown that temperature-enhanced growth of vascular plants in these typically moss-dominated ecosystems may promote microbial peat decomposition by increased C input via root exudates. To what extent different plant functional types (PFT) and soil temperature interact in controlling root C input is still unclear. In this study we explored how root C input is related to the presence of ericoid shrubs (shrubs) and graminoid sedges (sedges) by means of a factorial plant clipping experiment (= PFT effect) in two peatlands located at different altitude (= temperature effect).〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉By selective clipping of shrub and sedge shoots in mixed vegetation at two Alpine peatland sites we interrupted the above- to belowground translocation of C, thus temporarily inhibiting root C release. Subsequent measurements of soil respiration, dissolved organic carbon (DOC) concentration and stable isotope composition (〈sup〉13〈/sup〉C) of DOC in pore water were used as proxies to estimate the above- to belowground transfer of C by different PFT.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉We found that soil respiration rates and DOC concentrations temporarily decreased within 24 h after clipping, with the decrease in soil respiration being most pronounced at the 1.4 °C warmer peatland after clipping shrubs. The transient drop in DOC concentration coincided with a shift towards a heavier C isotope signature, indicating that the decrease was associated with inhibition of a light C source that we attribute to root exudates. Together these results imply that shrubs translocated more C into the peat than sedges, particularly at higher temperature.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉We showed that plant functional type and temperature interact in controlling root C input under field conditions in peatlands. Our results provide a mechanistic evidence that shrubs may potentially promote the release of stored soil C through root-derived C input.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Root-rot disease, a catastrophic disease of 〈em〉Panax quinquefolium〈/em〉 L. causes yield reduction and serious economic losses. However, knowledge of the relationship between rhizosphere microbial community and root-rot disease is limited. This study is aim to test whether the bacteria and fungi community differed between the soil attached to healthy and rotten roots of American ginseng. Moreover, the effects of American ginseng cultivation for 4 years on changes of soil physiochemical properties and microbial community were also investigated.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉High-throughput sequencing (Illumina MiSeq) was used to investigate the difference of microbial communities in the soils of new farmland (C) and the rhizosphere soils around healthy (H) and root rot diseased ginseng (R).〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Cultivation of American ginseng for 4 years not only changed the soil physicochemical properties, but also significantly increased the richness of the soil bacteria and decreased the fungal richness and diversity. Compared with other genera, the bacterial genera 〈em〉Nitrospira〈/em〉 and the fungal genera 〈em〉Gibberella〈/em〉 and 〈em〉Podospora〈/em〉 were strongly enriched in the soil of new farmland. However, the relative abundance of 〈em〉Janthinobacterium, Nitrospira〈/em〉 and 〈em〉Pedomicrobium〈/em〉 in bacterial community, and 〈em〉Mrakia, Paradendryphiella, Sporopachydermia, Myrothecium〈/em〉 and 〈em〉Racocetra〈/em〉 in fungal community were significantly decreased after culture of American ginseng. The results also showed that the bacteria and fungi community differs between the soil attached to healthy and rotten roots of American ginseng. The richness indices of fungal community showed a significant decrease in rhizosphere soils of R comparing with H. The bacteria 〈em〉Rhodoplanes〈/em〉 and 〈em〉Kaistobacter〈/em〉 were the dominant genera in the H sample, whereas 〈em〉Sphingobium〈/em〉 was dominant in the R sample. Notably, 〈em〉Monographella〈/em〉 was significantly higher in the R sample (23.13%) than that of H sample (2.90%). In addition, the fungi 〈em〉Melanophyllum〈/em〉 and 〈em〉Staphylotrichum〈/em〉 were the most differently abundant in the H sample, whereas 〈em〉Mortierella〈/em〉 and 〈em〉Cistella〈/em〉 were the differently abundant genera in the R sample.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Our results indicate that cultivation of American ginseng changed the edaphic factors and the soil microbial community, and there are significant differences in the microbial community between the soil attached to healthy and rotten roots of American ginseng.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉 We evaluated the effect of 〈em〉Azospirillum brasilense〈/em〉 strain HM053 inoculation on maize seeds, a spontaneous mutant that excrete ammonium and fix nitrogen constitutively.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Maize was grown with different nitrogen (urea) concentration and inoculated with 〈em〉A. brasilense〈/em〉 Ab-V5 (Brazilian commercial strain) or HM053 strain in four field experiments, in three regions of Parana State, Southern Brazil. We evaluated yield components, nutrient content on leaves and grains and productivity during the crop cycle.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Inoculation with 〈em〉A. brasilense〈/em〉 strain Ab-V5 and HM053 associated with base fertilization (30 kg ha〈sup〉−1〈/sup〉 N) improved crop yield in all trials. Ab-V5 increased production between 2.2 to 10.4%, or 178.0 to 759.9 kg ha〈sup〉−1〈/sup〉, respectively. HM053, by itself, increased production between 4.7 to 29%, or 460.5 to 1769.3 kg ha〈sup〉−1〈/sup〉, respectively.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉The new strain HM053 showed to be a great biofertilizer for maize seeds and a new alternative for a more sustainable agriculture.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Chemical stabilization is extensively used to improve the physical and engineering properties of problematic soils. This research was carried out to investigate the strength and microstructural properties of organic soil treated with a biomass silica stabilizer, named SH-85. The SH-85 content studied was varied at 3, 6, 9, 12, and 15% based on the organic soil weight. Compaction and unconfined compression strength tests were carried out to ascertain the strength improvement of the stabilized organic soil. The field-emission scanning electron microscopy (FESEM) and energy-dispersive X-ray spectrometry (EDAX) were carried out to examine the growth of cementation products. The cementation products filled large and small pores and welded soil particles, which results in strong interparticle forces. Therefore, the strength of treated soil is 5–20 times higher than that of the untreated soil.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉X-ray computed tomography (CT) is widely recognized as a powerful tool for in-situ quantification of root system architecture (RSA) in soil. However, employing X-ray CT to identify the spatio-temporal dynamics of RSA still remains a challenge due to non-automatic, time-consuming image processing protocols and their poor recovery of fine roots in soil.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Here we present a new protocol (Rootine) to segment roots rapidly and precisely down to fine roots with two voxels in diameter (90 μm in pots with 70 mm in diameter). This is facilitated by feature detection of the tubular shape of roots, an approach that was originally developed for detecting blood vessels in medical imaging.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉In comparison to established root segmentation methods, Rootine produced a more accurate root network, i.e. more roots and less over-segmentation. Root length quantified by X-ray CT showed high correlation with results by root washing combined with 2D light scanning (R〈sup〉2〈/sup〉 = 0.92). Tests with different soil materials showed that the recovery of roots depends on signal-to-noise ratio but can be up to 99% for a favorable contrast between fine roots and background.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉This new protocol provides great efficiency to study RSA in undisturbed soil. As it is fully automated it has the potential for high-throughput root phenotyping and related modelling.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Litter inputs are closely related to both forest productivity and nutrient cycling under climate change and local management. This study investigated the effect of litter inputs on litter decomposition, changes in litter chemistry and nitrogen (N) dynamics during eucalyptus leaf litter decomposition.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Two parallel in situ litter decomposition experiments were conducted at two sites with high-quality (HQ) and low-quality (LQ) litters in a eucalyptus-dominated forest of southeast Queensland, Australia. At each site, leaf litters with either a single (SL) or double mass load (DL) of litter inputs were decomposed for 15 months. Litter mass loss, chemical composition and N content of decomposing litters were measured seasonally during the decomposition period. The chemical composition of the collected litters was determined by solid-state 〈sup〉13〈/sup〉C nuclear magnetic resonance (NMR) spectroscopy.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The HQ litters decomposed faster than the LQ litter, with a decomposition constant of 0.53 and 0.33 y〈sup〉−1〈/sup〉 at the HQ and LQ site, respectively. Litter addition rates had no effect on litter decomposition, changes in chemical composition and N content during decomposition regardless of differences in initial litter quality. The HQ and LQ litters showed the same pattern of chemical changes during decomposition, with an increase in alkyl C and a decrease in di-O-alkyl C and aryl C. The relative intensity of O-aryl C and carboxyl C converged, while the relative intensity of di-O-alkyl C and 〈em〉δ〈/em〉〈sup〉15〈/sup〉N diverged as the decomposition progressed. N immobilization during decomposition depended on litter quality, with N consistently immobilized in LQ litters over the whole decomposition period.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉In subtropical eucalyptus-dominated forests, the dynamics of organic C and N during litter decomposition were resistant to the increased inputs of aboveground litters. Litter chemistry of different initial qualities converged at the early stages of decomposition, and the implications of chemical convergence on the formation and stabilization of soil organic matter need to be assessed in the future.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Previous studies suggest that organic carbon (C) and nitrogen (N) can stimulate soil nitrification, but whether autotrophic or heterotrophic nitrification is stimulated and who are active nitrifiers for the nitrification activity is still in debate. We elucidated which nitrification dominated and the active nitrifiers during the decomposition of rice callus.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉〈sup〉15〈/sup〉N-labeled callus and acetylene (C〈sub〉2〈/sub〉H〈sub〉2〈/sub〉) inhibition were used to explore the autotrophic or heterotrophic nitrification during the decomposition of callus and DNA-based stable isotope probing (SIP) and high-throughput sequencing were used to investigate the active nitrifiers.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Autotrophic nitrification dominated the nitrification activity, driven by oxidation of ammonia (NH〈sub〉3〈/sub〉) produced from mineralization of the callus-derived organic N. Callus significantly stimulated nitrification activity, which was paralleled by changes in the abundance and community composition of AOA. DNA-SIP further demonstrated that the active AOA outnumbered their bacterial counterparts in the 〈sup〉13〈/sup〉C-DNA from the soil with callus amendment. Phylogenetic analysis revealed the functional importance of soil fosmid 29i4-like and 54d9-like AOA within soil group 1.1b during the active nitrification with callus cells.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉NH〈sub〉3〈/sub〉 released from the mineralization of callus was the main substrate for autotrophic nitrification and preferentially stimulated the growth of AOA within group 1.1b in the paddy soil.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Mercury (Hg) was used during 1929–1950 as a catalyst to produce rayon acetate at the former DuPont plant in Waynesboro, Virginia, and released into the South River. Though the use of Hg ceased in the 1970s, the affected ecosystem is still a matter of concern. Here, high total mercury (THg) and total organomercury, stated as methylmercury (MeHg) are reported in historically contaminated floodplain soils and shallow groundwater from five locations sited 5.6 km downstream from the plant of one river edge site. In soils, THg ranged from 0.1 to 1201.5 mg/kg exceeding the health-based screening Hg levels for residential and industrial soils while MeHg varied between 0.1 and 54 µg/kg. Concentrations decreased sharply with depth indicating the stratification of legacy industrial Hg-rich soils underlain by the pre-industrial soils with minor Hg. Strong linear correlation between THg and MeHg was observed. Highest soil MeHg was associated with total carbon, poorly crystalline and amorphous Fe and/or Mn oxyhydroxides. Sequential extraction analyses indicated that Hg was present mostly in relatively recalcitrant forms, as determined with procedures that targeted β-HgS, HgS, HgSe, HgAu, thiol-bound Hg, Hg〈sup〉0〈/sup〉, and some organo-complexed Hg, Hg〈sub〉2〈/sub〉Cl〈sub〉2〈/sub〉 phases. High Cu (≤ 404.3 mg/kg), Zn (≤ 151.3 mg/kg), Cr (≤ 123.9 mg/kg) were also identified. In shallow groundwater, THg ranged from 28 to 538 ng/L and MeHg varied between 1.2 and 137 ng/L. During the intermittent precipitation, the highest MeHg was linked to the highest Fe〈sup〉2+〈/sup〉, Mn, SO〈sub〉4〈/sub〉〈sup〉2−〈/sup〉, total alkalinity, and conductivity, which could be due to either the potential leaching and dissolution of soil minerals and/or the saturation of the vadose zone. A sharp increase in the soil moisture at the top 40–70 cm of the Hg-rich soils after rainfall and overbank flooding was followed by redox gradients from oxidizing (≈ + 600 mV) to reducing (≈ − 300 mV) and a reverse response in a transmissive gravel zone at the base of the bank (≈ − 400  to + 200 mV) with a defined lag with depth. These dynamic seasonal fluctuations at the South River might be crucial for solubilization of Hg, Fe, and Mn redox-sensitive minerals triggering the anoxic response for new MeHg production.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and Aims〈/h3〉 〈p〉Rare earth elements (REE) are a group of the periodic table formed by 17 chemical elements (lanthanoids plus yttrium and scandium). They have been used in different field applications. In agriculture, they can be found in some phosphate fertilizers at levels one or two orders of magnitude higher than those found in normal agricultural soils. Citrus plants are known to present high levels of REE when compared to most other species, however, there is little information about bioavailability of REE in phosphate fertilizers for citrus plants. This work focuses on the study of REE behavior by the application of increasing doses of single superphosphate fertilizer in Rangpur lime (〈em〉Citrus limonia〈/em〉 Osbeck) plants in a greenhouse study.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉The technique used was instrumental neutron activation analysis (INAA).〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The results showed that the fertilizer has caused significant increases in the content of REE in the citrus plant tissues, with higher concentrations in leaves than in branches. The highest substrate-leaf transfer factor was observed for La (0.0047), though the concentrations in the plants followed the same order found in the substrate, i.e. Ce 〉 La 〉 Sm 〉 Eu 〉 Sc.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉There was an increase of rare earth elements concentrations in Rangpur lime plants by superphosphate fertilizer application.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Soil alkalization imposes severe ion toxicity, osmotic stress, and high pH stress to plants, inhibiting their growth and productivity. NaHCO〈sub〉3〈/sub〉 is a main component of alkaline soil. However, knowledge of the NaHCO〈sub〉3〈/sub〉-responsive proteomic pattern of alkaligrass is still lacking. Alkaligrass (〈em〉Puccinellia tenuiflora〈/em〉) is a monocotyledonous halophyte pasture widely distributed in the Songnen Plain in Northeastern China. This study aims to investigate the NaHCO〈sub〉3〈/sub〉-responsive molecular mechanisms in the alkaligrass plants.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉An integrative approach including photosynthetic and redox physiology, and comparative proteomics was used.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉NaHCO〈sub〉3〈/sub〉 decreased photosynthesis, but increased nonphotochemical quenching, increased membrane electrolyte leakage of alkaligrass, and increased proline and glycine betaine concentrations in leaves. In addition, the NaHCO〈sub〉3〈/sub〉 stress increased Na〈sup〉+〈/sup〉 concentration and decreased K〈sup〉+〈/sup〉/Na〈sup〉+〈/sup〉 ratio in leaves, while Ca〈sup〉2+〈/sup〉 and Mg〈sup〉2+〈/sup〉 concentrations were maintained, contributing to signaling and homeostasis of ion and enzyme activity. Furthermore, O〈sub〉2〈/sub〉〈sup〉−〈/sup〉 generation rate and H〈sub〉2〈/sub〉O〈sub〉2〈/sub〉 concentration were increased, and the activities of ten antioxidant enzymes and antioxidant concentrations were changed in response to the NaHCO〈sub〉3〈/sub〉 stress. Proteomics revealed 90 NaHCO〈sub〉3〈/sub〉-responsive proteins, 54% of which were localized in chloroplasts. They were mainly involved in signaling, photosynthesis, stress and defense, carbohydrate and energy metabolism, as well as protein synthesis, processing and turnover. Some protein abundances did not correlate well with their activities, implying that the enzyme activities were affected by NaHCO〈sub〉3〈/sub〉-induced post-translational modifications.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉To cope with the NaHCO〈sub〉3〈/sub〉 stress, alkaligrass deployed multiple strategies, including triggering phospholipase D (PLD)-mediated Ca〈sup〉2+〈/sup〉 signaling pathways, enhancing diverse reactive oxygen species (ROS) scavenging pathways, and regulating chloroplast protein synthesis and processing.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉The method is proposed for assessing water consumption in the transboundary basin under the scarce available data on exploitation of water resources. The method is based on analysis of the dynamic rain–flow relationship which confirms the consistent flow decline during subsequent sub-periods as a result of the increased water consumption. The proposed method was applied to the Yarmouk River Basin up to the Maqaren Station. The study area (6058 km〈sup〉2〈/sup〉) includes Syrian (more 80%) and Jordanian territories. The present study provided complete information on consumption from the Yarmouk River in Syria (1971–2009) and confirmed its significant impact on flow reaching Jordan (downstream area) and on aquifer discharge through springs. During 1997–2009 the total water consumption was estimated as 76% of the Yarmouk Natural Flow vs. 24% for the measured flow at Maqaren Station. Similar estimates for surface water and for groundwater are 82% and 67% vs. 18% and 23%, respectively. In 2006–2009, annual consumption (total, SW and GW) critically increased and reached to 95–100% of the Natural Flow. The perennial base flow of the Yarmouk River at Maqaren Station is caused by outflow of springs in the outlet section of the Hreer tributary, which represents the local aquifer discharge. Its mean annual value was assessed as 120 MCM (40% of the Yarmouk natural flow) but it decreased to almost complete dry-out in 2006–2009 under conditions of the decline in the water level of the basin aquifer and the depletion and drying of the springs as the result of the unrestrained increased pumping of groundwater. These results are useful for water balance estimation as well as for assessment and management of the water resources in Syria and Jordan. The method can be adapted to real-time monitoring of the water consumption from the Yarmouk River (Syria). The proposed method can be applied to other areas with problems of the transboundary water relations under scarce water resources and limited data on the water use.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉This study aims to quantify soil organic C density (SOCD) in tropical semiarid mangroves and to compare these results to SOCD under the main tropical semiarid vegetation units (VU), aiming to better understand the role of semiarid mangroves in C sequestration, as well as to provide a basis for a better quantification of human impacts on this ecosystem. SOCD was quantified in two sets of calculations: at a 40-cm soil depth and considering the same soil mass (equivalent SOC). Mangroves can be considered the main organic C sink in the tropical semiarid environment, storing twice as much organic C per area as semiarid VUs, when considering the upper 40-cm soil layer and almost 6 times as much organic C per area when considering the same soil mass. Anthropogenic impacts (disposal of nutrients and vegetation removal) on the studied mangroves decreased SOCD and resulted in a 4.94 ± 0.74 Tg CO〈sub〉2〈/sub〉 emission, equivalent to 5.2% of the Brazilian annual CO〈sub〉2〈/sub〉 emission by fossil fuels. Compared to mangroves from tropical humid regions, SOCD in tropical semiarid mangroves is considerably lower.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈p〉Understanding the mechanisms and processes of groundwater recharge and evolution is critical for sustainable water resources management to meet human and agriculture needs under climate change, because groundwater is the primary water source in semiarid and arid regions, where the surface water resources are usually highly unstable and scarce. However, few studies investigated the recharge and evolution processes of groundwater combining with isotopic geochemistry and radiocarbon data, especially focused on the interactions among precipitation, surface water, groundwater, and rock. This study examined the recharge and evolution processes of groundwater in the Wuwei Basin based on stable isotopes, chemical indicators, and radiocarbon data. Our results showed that the Na〈sup〉+〈/sup〉 (sodium ion) and K〈sup〉+〈/sup〉 (potassium ion) concentrations of the groundwater were controlled by the dissolution of sylvite and halite origin from sediments, whereas the increase of Na〈sup〉+〈/sup〉 and Cl〈sup〉−〈/sup〉 (chloride ion) concentrations were not in accordance with a ratio of 1:1, indicating that the Na〈sup〉+〈/sup〉 and K〈sup〉+〈/sup〉 concentrations in groundwater were barely affected by the dissolution of halite and sylvite. Meanwhile, we also found that bicarbonate ion (HCO〈sub〉3〈/sub〉〈sup〉−〈/sup〉) was the dominant ion with a decreased ratio in the groundwater. The SO〈sub〉4〈/sub〉〈sup〉2−〈/sup〉/Cl〈sup〉−〈/sup〉 (sulfate ion/chloride ion) ratio decreased with the sample profile from Southwest to Northeast due mainly to the increases of Cl〈sup〉−〈/sup〉 concentration. The Ca〈sup〉2+〈/sup〉/Cl〈sup〉−〈/sup〉 (calcium ion/chloride ion) ratio decreased with the enhancement of Cl〈sup〉−〈/sup〉 in the hydrodynamic sluggish belt. In addition, the δ〈sup〉18〈/sup〉O (oxygen isotope) and δ〈sup〉2〈/sup〉H (hydrogen isotope) values of groundwater gradually increased from Southwest to Northeast along the flow path. The heavy isotopic values were more strongly depleted than the isotopic values of precipitation in the ground water samples, suggesting that the recharge of ground water in the plain region was very limited from precipitation. Moreover, the groundwater in the phreatic aquifer was younger water with 〈sup〉3〈/sup〉H (tritium isotope) values from 47 to 71 a.BP (before present), while the groundwater age in the confined aquifer was 1000–5800 BP evidenced by the 〈sup〉14〈/sup〉C (carbon isotope) values between 48 and 88 pmc (percentage modern carbon content). Overall, these results suggested that the unconfined groundwater enriched along the overall groundwater flow path from the southwest to northeast of the Wuwei Basin and the melt water from the Qilian Mountains may determine the water isotopic composition and contributed to the steady long-term runoff of the Shiyang River. Our findings may have important implications for inter-basin water allocation programmes and groundwater management in the Wuwei Basin.〈/p〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉The selection and breeding of low grain-cadmium (Cd)-accumulating rices is a promising approach for reducing the Cd concentration in grains. A cadmium-safe rice line designated D62B (〈em〉Oryza Sative〈/em〉 L.) accumulated a low Cd concentration in brown rice for safe consumption (〈 0.2 mg kg〈sup〉−1〈/sup〉). D62B was a great potential breeding material with a weaker translocation capacity of Cd to shoot compared with common rice lines. A prior understanding of the Cd translocation mechanisms in D62B offered theoretical basis for breeding new low-Cd-accumulating rice cultivars in the future.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉A pot experiment was conducted to investigate the changes of non-protein thiols in shoot and root of D62B in comparison with a common rice line (Luhui17), and then the relationship between the Cd translocation via xylem and organic acids in xylem sap of two rice lines was explored by a hydroponic experiment.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉D62B showed lower Cd translocated to shoot in comparison with Luhui17. The translocation factor (TF) of D62B varied from 0.11 to 0.15. Cd exposure promoted the synthesis of non-protein thiols in two rice lines, particularly in roots. Syntheses of glutathione (GSH) and phytochelatins (PCs) in root of D62B were greater than those of Luhui17. Cd concentration in xylem sap of D62B was significant positively correlated with the malic acid, citric acid and tartaric acid concentrations. The citric acid and tartaric acid concentrations in xylem sap of D62B were significantly lower than those of Luhui17. There was no significant difference for malic acid between two rice lines.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉More GSH and PCs in root of D62B was beneficial to Cd retention in root. Furthermore, the involvement of lower citric acid and tartaric acid in Cd translocated in xylem sap of D62B resulted in lower Cd accumulation in shoot.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Microbial denitrification is the primary driver of nitrogen losses from the plant-soil system and the key process for the closure of the global N cycle. All major controls of denitrification might be directly or indirectly affected by plants. However, there is a lack of research of the direct effects of plants on soil denitrification and how this effect might be mediated by soil properties. This study assesses the effect of three common crop species and two agricultural soils on denitrification potentials.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉We conducted a factorial experiment under controlled conditions to analyze the effects of (1) different plant species (barley, wheat or ryegrass), (2) two different soils (texture/ SOC) and (3) two different soil moisture levels on Denitrification Enzyme Activity (DEA) in bulk and rhizosphere soil.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉The SOC richer clay loam soil showed on average higher DEA (+81%) compared to the SOC poorer silty loam soil. All three plants were found to stimulate denitrification with significant differences between certain species: rye grass (+92% ± 14%) ≥ barley (+75% ± 26%) ≥ wheat (+50% ± 19%).〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉DEA in agricultural soils is interactively controlled by plant species and soil type with an overall stimulating effect of plants on the denitrification potential. Future research should focus on disentangling single mechanisms of plant control on actual denitrification rates and N gas product ratios.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background〈/h3〉 〈p〉Biocrusts are important functional units in dryland ecosystems. Regarded as ecosystem engineers, cyanobacteria in biocrusts contribute several major physico-chemical and biological processes. However, the role of cyanobacteria in the process of loess formation has been underestimated. Recently, their contribution to sediment development was presented in the BLOCDUST model of loess formation.〈/p〉 〈/span〉 〈span〉 〈h3〉Scope〈/h3〉 〈p〉This 〈em〉perspective paper〈/em〉 features the environmental impact of cyanobacteria and biocrusts with a focus on processes involved in the formation of loess sediments. We propose that the formation of loess can be mediated by cyanobacteria, including initial trapping, and the accumulation and preservation of loess-forming particles. Moreover, the initial structure may be further altered by weak mineral weathering, dissolution and mineral re-precipitation due to cyanobacterial metabolic processes. Possible negative aspects of environmental impact related to the potential toxicity of cyanobacterial biocrusts are also discussed. We highlight specific biotic-abiotic interactions between biocrusts and loess (e.g. exudation of organic polymers, carbonate dissolution and re-precipitation, and dust-dependent metabolic activities of cyanobacteria) which are essential for the formation of stabilized loess and propose the term “synergosis” to comprise these interactions.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉The role of cyanobacteria in loess formation has only recently been recognized and the possible biogenic nature of loessification is underestimated as compared to their eolian nature. Mineral weathering and mineral precipitation processes as well as mineral dust flux between litho- and atmosphere mediated by cyanobacteria and biocrusts require more attention due to their significant contribution to ecosystem properties.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉The objective of this paper was to develop a method based on infrared spectroscopy to compare mineral content in soils and apply it to evaluate soil mineralogical variations in pairs of inter-patch and patch soils in a semi-arid area.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉Mixtures of several minerals were analyzed by infrared spectroscopy, the second derivative of the spectra was calculated and the spectra normalized respect to calcite or quartz signals (711 cm〈sup〉−1〈/sup〉 or 800 cm〈sup〉−1〈/sup〉 respectively). The intensities of representative signals of each mineral were related to their concentration in the mixtures. Pairs of patch and inter-patch soils from five different sites were analyzed by this method. Elemental analysis and total lime analysis were performed in some soil pairs.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Soils were dominated by calcite and quartz, or by montmorillonite and kaolinite. Inter-patch soils were richer in calcite and poorer in quartz or clays than patch soils. Calcite losses in patch soils might be related to soil acidification by CO〈sub〉2〈/sub〉 from respiration and/or organic matter. Elemental analysis showed high values of S, Cl, and K in patch soils with respect to inter-patch soils.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉The proposed FTIR method was useful to compare soil mineralogy in specific areas. Fertile spots by accumulation of water, soluble salts and sediments may favor plant growth in semi-arid regions and these plants may increase the fertility of the spot. Changes in soil mineral composition could be used to monitor the biological activity of soil in arid and semi-arid zones.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉Waterlogging is a common natural disturbance that has negative impacts on dry-land plant species. However, few studies have focused on how waterlogging influences the invasiveness of non-native plant species on dry lands. 〈em〉Bidens pilosa〈/em〉 is an invasive dry-land plant of the Asteraceae family that causes serious damage to biodiversity and agricultural production in southern China. To date, it remains unclear how waterlogging affects the competitiveness and growth of 〈em〉B. pilosa〈/em〉. The goal of this study is to determine whether waterlogging promotes the competitiveness of invasive 〈em〉B. pilosa〈/em〉.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉The growth and physiological responses of invasive 〈em〉B. pilosa〈/em〉 and native 〈em〉B. biternata〈/em〉 and the competition effects between them were studied after 0 (control), 5, 10, 15, and 20 days of waterlogging stress (wherein the water level was maintained at the soil surface level).〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉After short-term waterlogging stress, the competitive balance index of invasive 〈em〉B. pilosa〈/em〉 significantly increased, indicating that short-term waterlogging on dry lands could significantly improve the competitiveness of invasive 〈em〉B. pilosa〈/em〉. Invasive 〈em〉B. pilosa〈/em〉 maintained more rapid adventitious root generating capacity and higher root dehydrogenase activity under waterlogging conditions than native 〈em〉B. biternata〈/em〉, which allowed 〈em〉B. pilosa〈/em〉 to adapt to the anoxic conditions much more rapidly. The smaller reductions in net photosynthetic rate, actual quantum yield of photosystem II and relative growth rate in 〈em〉B. pilosa〈/em〉 than in 〈em〉B. biternata〈/em〉 showed that invasive 〈em〉B. pilosa〈/em〉 had stronger tolerance to waterlogging than native 〈em〉B. biternata〈/em〉.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉Our results indicate that invasive 〈em〉B. pilosa〈/em〉 has stronger tolerance to waterlogging than native 〈em〉B. biternata〈/em〉 and that short-term waterlogging on dry lands can significantly improve the competitiveness of invasive 〈em〉B. pilosa〈/em〉. Short-term waterlogging on dry lands caused by extreme precipitation during the rainy season is expected to promote the invasive potential of exotic 〈em〉B. pilosa〈/em〉.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Aims〈/h3〉 〈p〉Endophytes benefit host plants by increasing biotic and abiotic stress tolerance. The aims of this study were to evaluate endophytic community (EC) of 〈em〉Arabis alpina〈/em〉, a Pb-Zn hyperaccumulator and investigate role of EC in host plants metal tolerance.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉EC of 〈em〉A. alpina〈/em〉 growing at Pb–Zn mining area was evaluated by Illumina MiSeq sequencing. Pot experiments were conducted for the role of EC in metal accumulation and tolerance of host.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Fungal EC of shoots showed greater similarity to roots than to seeds; and Chao1 and Shannon indices for shoots and roots were significantly higher than for seeds. Inoculation of EC significantly improved host plants growth under multi-metal stress (〈em〉p〈/em〉 〈 0.05, T test). The shoot length, root length and dry biomass of the treatment were improved when compared with the control. EC inoculation significantly altered accumulation of Pb, Cd and Zn in plant tissues. Particularly decreased the accumulation of Pb (〈em〉p〈/em〉 〈 0.05) and Cd (〈em〉p〈/em〉 〉 0.05) in the shoots of the treatment.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusions〈/h3〉 〈p〉Hyperaccumulator 〈em〉A. alpina〈/em〉 growing in metals contaminated soils was colonized by a diverse assemblage of endophytic fungi, and the EC played a key role in increasing host plants metal tolerance.〈/p〉 〈/span〉

Beschreibung: 〈h3〉Abstract〈/h3〉 〈span〉 〈h3〉Background and aims〈/h3〉 〈p〉The incidence of extreme weather events, particularly drought is predicted to increase in the future and alter the ecosystem process. Despite that the interplay between plant species play a critical role in reducing the vulnerability of soil ecosystem to drought, whether the presence of legumes in plant community could maintain nutrient uptake of focal species by stabilizing soil biota and ecosystem processes under drought conditions remains essentially unexplored.〈/p〉 〈/span〉 〈span〉 〈h3〉Methods〈/h3〉 〈p〉In a field experiment, the soil biota community and ecosystem processes were studied using four planting systems contain monoculture of focal species 〈em〉Zanthoxylum bungeanum〈/em〉, mixed cultures of 〈em〉Z. bungeanum〈/em〉 and 〈em〉Capsicum annuum〈/em〉, 〈em〉Z〈/em〉. 〈em〉bungeanum〈/em〉 and 〈em〉Medicago sativa,〈/em〉 and 〈em〉Z. bungeanum〈/em〉 and 〈em〉Glycine max〈/em〉 subjected to drought.〈/p〉 〈/span〉 〈span〉 〈h3〉Results〈/h3〉 〈p〉Drought had no significant effects on soil microbial biomass in monoculture and mixed cultures, but significantly increased microbial stress indices. Drought significantly increased the densities of total nematodes, herbivores, bacterivores and fungivores in 〈em〉Z〈/em〉. 〈em〉bungeanum〈/em〉 and 〈em〉M. sativa〈/em〉 mixed culture, but significantly decreased the total nematodes, bacterivores and fungivores in 〈em〉Z. bungeanum〈/em〉 and 〈em〉G. max〈/em〉 mixed culture. Under drought stress, leaf nitrogen concentrations of 〈em〉Z. bungeanum〈/em〉 were significantly higher in 〈em〉Z. bungeanum〈/em〉 and 〈em〉M. sativa〈/em〉 mixed culture than 〈em〉Z. bungeanum〈/em〉 monoculture and the other mixed cultures, this is mainly due to higher microbial activity and net nitrogen mineralization rate.〈/p〉 〈/span〉 〈span〉 〈h3〉Conclusion〈/h3〉 〈p〉Differences in resistance traits of neighbors had additive effects and rapidly reflected in different soil ecosystem processes and nutrient uptake of focal species. Our results revealed that specific legume species intercropping management could stabilize focal species by maintaining soil ecosystem processes under drought condition.〈/p〉 〈/span〉