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  • 36.40  (73)
  • Immunocytochemistry  (46)
  • stress  (34)
  • Springer  (153)
  • American Institute of Physics
  • Institute of Physics
  • 2010-2014
  • 1985-1989  (153)
  • 1965-1969
  • 1940-1944
  • 1989  (153)
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Verlag/Herausgeber
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  • 2010-2014
  • 1985-1989  (153)
  • 1965-1969
  • 1940-1944
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 53 (1989), S. 287-296 
    ISSN: 1570-7458
    Schlagwort(e): European corn borer ; Ostrinia nubilalis ; maize ; water ; drought ; stress ; development ; models ; phytotron ; temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Des plants de maïs se développent dans un phytotron dans 4 conditions d'humidité du sol (de la saturation à la dessication) et à 3 températures constantes (20°, 25° & 30°C). Chaque pied est contaminé au moment de l'émission du pollen, par une ooplaque d'O. nubilalis Hübn. (ECB) de race européenne E. L'installation, la colonisation et le développement des chenilles sont notés lors de 12 périodes de prélèvements destructifs (4 par température). La vitesse de développement d'O. nubilalis est affectée par la température, main non par l'humidité du sol. Les 4 niveaux d'humidité du sol n'ont aucun effet sur la teneur en eau des tiges de maïs. En fait, les feuilles de maïs présentent une senescence précoce lorsqu'il y a déficit en eau dans le sol. La teneur en eau du sol agit sur l'installation, sur la distribution verticale, la dispersion et le lieu d'alimentation des chenilles; mais ces effets sont légers et ne modifient pas la vitesse de développement. L'environnement larvaire dans la tige de maïs est efficacement isolé des variations externes par l'aptitude de la plante à maintenir la teneur en eau des tiges relativement élevée et stable. Ainsi, des changements importants au niveau du sol n'ont pratiquement pas d'effets sur le développement d'O. nubilalis, malgré les conséquences brutales pour la plante. Cette étude montre que la vitesse de développement d'O. nubilalis est relativement insensible aux modifications de la teneur en eau du sol ainsi qu'aux effets de ce stress de sécheresse sévère sur le pied de maïs. La discussion porte sur l'importance de ces résultats pour la modélisation de la dynamique de l'insecte, la physiologie de la culture et les interactions entre insecte et plante.
    Notizen: Abstract Maize plants were grown under four moisture regimes (wet to extreme deficit) and three constant temperatures (20°, 25° & 30°C) in a phytotron. Each plant was infested with one E-race European corn borer [Ostrinia nubilalis (Hubn.)] (ECB) egg mass at pollen shed. ECB development, location, and establishment were recorded over the course of 12 destructive sample dates (4/temperature). ECB developmental rates were not significantly affected by soil moisture treatments, but were significantly affected by temperature. In spite of successful establishment of four distinctly different soil moisture regimes, the maize stalk tissue water levels were not significantly different among soil water treatments. Instead, the maize plants exhibited accelerated leaf senescence in response to the water deficit conditions. Among the soil water treatments, differences were found in larval establishment, vertical distribution and dispersion, and feeding site selection; however, those effects were slight and could not explain the similarity in ECB developmental rates observed in these treatments. In maize, the larval environment within the stalk was effectively insulated from changes in the external environment by the plant's ability to maintain a relatively high and stable stalk tissue water content. Thus, large changes to the soil environment had essentially no effect on ECB development, though drastic consequences for the plant. This study indicates that ECB rates of development are relatively insensitive to changes in the soil water environment as well as the associated changes in the maize plant that accompany severe drought stress. The significance of these findings to insect modelling, crop physiology, and insect-crop interactions is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    European journal of nutrition 28 (1989), S. 49-55 
    ISSN: 1436-6215
    Schlagwort(e): ascorbic acid metabolism ; vitamin C serum level ; stress ; C allitrichidae ; Ascorbinsäurestoffwechsel ; Vitamin C Serumgehalt ; Streß ; C allitrichidae ; Krallenaffen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die NeuweltaffenCallithrix jacchus, Marmosets, undSaguinus fuscicollis, Tamarine, sind, wie andere Affen auch, auf die externe Zufuhr von Ascorbinsäure angewiesen. Um bei Marmosets einen Serumspiegel oberhalb der Nierenschwelle zu erreichen, müssen ihnen täglich 15 mg/kg metabolischem Körpergewicht zugeführt werden. Die Nierenschwelle liegt bei ihnen im gleichen Bereich wie beim Menschen. Ein Vergleich des Ascorbinsäurespiegels zwischen den beiden Spezies aus einer Zuchtkolonie ergab einen gravierend niedrigeren Wert bei den Tamarinen. Bei beiden Spezies wurde Ascorbinsäure durch ungewohnte Versuchsbedingungen oder zusätzliche Streßfaktoren zu einem höheren Prozentsatz zu CO2 abgebaut, als wenn die Tiere an den Stoffwechselkäfig gewöhnt waren. Diese Isotopenexkretionsversuche mit14C-markierter Ascorbinsäure weisen auf ein unterschiedliches Stoffwechselverhalten von Ascorbinsäure zwischen den beiden Spezies hin, was vermutlich durch die höhere Streßanfälligkeit der Tamarine bei vergleichbaren Bedingungen verursacht ist.
    Notizen: Summary Like other simian primates, the New World monkeyCallithrix jacchus, marmoset, andSaguinus fuscicollis, tamarin, require ascorbic acid as an essential nutrient. For adult marmosets, a daily intake of 15 mg/kg metabolic body weight was found to be necessary to obtain a serum level above the kidney threshold. A survey of the serum ascorbic acid level of marmosets and tamarins in a breeding colony resulted in a vast divergence between the two species, indicating a higher ascorbic acid requirement for tamarins. Unaccustomed trial conditions or additional Stressors resulted in a higher catabolism of ascorbic acid to CO2 in both species, measured with14C labeled material, compared to a higher rate of renal excretion when the animals were accustomed to the metabolic cage. These isotope excretion studies suggest a different metabolic behavior of ascorbic acid in the two species. This is supposedly caused by a higher sensitivity of the tamarins when subjected to the same conditions as marmosets.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 45 (1989), S. 788-798 
    ISSN: 1420-9071
    Schlagwort(e): Brain asymmetry ; rotational behavior ; genetic models ; testosterone ; brain development ; stress ; strain differences ; sexual dimorphism ; hemispheric differences
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Cerebral laterality can no longer be considered an exclusively human trait, as over the last 15 years there has been an emergence of data to suggest that animal brains are also lateralized. Morphologic, chemical and behavioral indices of brain asymmetry in the rodent have been reported, and it is suggested that variations in the magnitude and direction of these indices are determined by a complex interaction of genetic, hormonal and experiential factors. Interindividual differences in cerebral laterality have been shown to covary with, or predict, individual differences in spatial behavior and stress reactivity, as well as susceptibility to stress pathology and drug sensitivity. Such findings suggest that it is possible to study individual differences in lateralized brain function through the use of animal models.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 45 (1989), S. 798-805 
    ISSN: 1420-9071
    Schlagwort(e): AA and ANA rats ; alcohol consumption ; genetic selection ; neurochemistry ; stress ; alcohol withdrawal ; behavior ; ethanol metabolism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The offspring of rats that voluntarily select larger quantities of alcohol are heavier consumers of alcohol than the offspring of rats that tend to avoid it. Such selective breeding, repeated over many generations, was used to develop the AA (Alko, Alcohol) line of rats which prefer 10% alcohol to water, and the ANA (Alko, Non-Alcohol) line of rats which choose water to the virtual exclusion of alcohol. In addition to demonstrating the likely role of genetic factors in alcohol consumption, these lines have been used to find behavioral, metabolic, and neurochemical correlates of differential alcohol intake. Some of the line differences that have been found involve the reinforcing effects of ethanol, the changes in consumption produced by alcohol deprivation and nutritional factors, the behavioral and adrenal monoamine reactions to mild stress, the development of tolerance, the accumulation of acetaldehyde during ethanol metabolism, and the brain levels of serotonin. It is hoped that these studies will lead to a better understanding of the genetically-determined mechanisms that influence the selection of alcohol.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 91 (1989), S. 3-11 
    ISSN: 1573-4919
    Schlagwort(e): calcium ATPase ; glucose transport ; herpes virus ; hypertension ; leaks ; pore-formation ; sodium channels ; stress ; toxins ; transport ; viruses
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Four situations in which membrane transport is altered by disease are discussed: (a) non-specific leaks induced by poreforming agents; (b) glucose transport and cellular stress; (c) Ca+-ATPase and hypertension; (d) Na− channels and HSV infection.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 258 (1989), S. 119-124 
    ISSN: 1432-0878
    Schlagwort(e): Development, ontogenetic ; Immunocytochemistry ; Monoclonal antibodies ; Mucosa ; Lymphoid organs ; Domestic fowl
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The postnatal development of chicken mucosa-associated lymphoid tissues of the eyes, lungs, and intestines were investigated with monoclonal antibodies specific for either all leucocytes, B lymphocytes, mononuclear phagocytes, IgM, IgG, or IgA. Attention has been paid to the relation of lymphoid infiltrates with their surrounding mucosae, the segregation into B-cell and T-cell areas, development of germinal centers, and secretory immunoglobulins. Abudant secretory IgM and IgA was detected in the epithelium of the Harderian glands in the orbits, even though they lacked large leucocyte infiltrates with germinal centers. Lymphoid tissues in the mucosae of lungs and intestines developed separate B-cell and T-cell areas. The proventriculus, Meckel's diverticulum, and Peyer's patches generally contained germinal centers from 12 weeks of age on. Because chickens as young as 2 weeks old had germinal centers in bronchus-associated lymphoid tissue and cecal tonsils, these areas were probably highly stimulated by antigens. Isotype-specific monoclonal antibodies were used to detect IgM-, IgG-, and IgA-bearing follicular cells in the same germinal center.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 1432-0878
    Schlagwort(e): Insect AKH/RPCH ; Neurohormones ; Cam-HrTH-II ; Lom-AKH-I ; Immunocytochemistry ; Carausius morosus, Sarcophaga bullata (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A polyclonal antiserum was prepared against an N-terminal modified Cam-HrTH-II (Leu-Asn-Phe-...), one of the members of the large AKH/RPCH peptide family, first isolated from Carausius morosus. The localisation of this peptide was performed by means of immunocytochemical methods in the brain and corpora cardiaca-corpora allata complex of the stick insect, Carausius morosus and the grey fleshfly, Sarcophaga bullata. The distribution patterns of molecules reactive to the Cam-HrTH-II and the LomAKH-I antisera in both insect species were compared. In Carausius, both antisera reacted in the same cell bodies. In Sarcophaga, some neurons were stained by both, others only by one of the two antisera. By combining two different antisera, we demonstrated that there are no Lom-AKH-I-like molecules present in Carausius and that there must occur at least three different AKH-like molecules in the brain of Sarcophaga. One is similar to Cam-HrTH-II, the second to Lom-AKH-I and the third is an AKH/RPCH-like peptide, different from Lom-AKH-I and Cam-HrTH-II.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 258 (1989), S. 1-24 
    ISSN: 1432-0878
    Schlagwort(e): Serotonin ; Immunocytochemistry ; Insect nervous system ; Protocerebrum ; Suboesophageal ganglion ; Manduca sexta (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Serotonin-immunoreactive neurons in the median protocerebrum and suboesophageal ganglion of the sphinx moth Manduca sexta were individually reconstructed. Serotonin immunoreactivity was detected in 19–20 bilaterally symmetrical pairs of interneurons in the midbrain and 10 pairs in the suboesophageal ganglion. These neurons were also immunoreactive with antisera against DOPA decarboxylase. All major neuropil regions except the protocerebral bridge are innervated by these neurons. In addition, efferent cells are serotonin-immunoreactive in the frontal ganglion (5 neurons) and the suboesophageal ganglion (2 pairs of neurons). The latter cells probably give rise to an extensive network of immunoreactive terminals on the surface of the suboesophageal ganglion and suboesophageal nerves. Most of the serotonin-immunoreactive neurons show a gradient in the intensity of immunoreactive staining, suggesting low levels of serotonin in cell bodies and dendritic arbors and highest concentrations in axonal terminals. Serotonin-immunoreactive cells often occur in pairs with similar morphological features. With one exception, all serotonin-immunoreactive neurons have bilateral projections with at least some arborizations in identical neuropil areas in both hemispheres. The morphology of several neurons suggests that they are part of neuronal feedback circuits. The similarity in the arborization patterns of serotonin-immunoreactive neurons raises the possibility that their outgrowing neurites experienced similar forces during embryonic development. The morphological similarities further suggest that serotonin-immunoreactive interneurons in the midbrain and suboesophageal ganglion share physiological characteristics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 258 (1989), S. 101-109 
    ISSN: 1432-0878
    Schlagwort(e): Serotonin ; Immunocytochemistry ; Subesophageal ganglion ; Neurohemal organs ; Manduca sexta (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Serotonin-immunoreactivity is mapped in wholemounts and slices of the suboesophageal ganglion (SOG) of larval Manduca sexta by means of immunocytochemistry. An extensive meshwork of serotonin-immunoreactive nerve fibres on some peripheral nerves of the SOG has been demonstrated. This meshwork appears to belong to a serotonergic neurohemal system, probably supplied by two pairs of bilateral serotonin-immunoreactive neurons with big cell bodies on the dorsal side near the midline in the mandibular neuromere. Intracellular recording and staining revealed their physiology and morphology. These neurons produce long lasting (50 msec) action potentials, which suggest that they are neurosecretory cells. Two pairs of bilateral serotonin-immunoreactive interneurons similar to those of other insects are stained in the labial and maxillar neuromeres, but not in the mandibular neuromere. Their ventrolaterally located cell bodies project through a ventral commissure into the contralateral hemiganglion and then cross back again through a dorsal commissure. The axons project into the contralateral circumoesophageal connective.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    ISSN: 1432-0878
    Schlagwort(e): Na+, K+-ATPase ; Immunocytochemistry ; Kidney ; Salivary glands ; Transport ; Rat (Sprague-Dawley)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary An antibody to the 96 kD α-subunit of the Na+, K+ -ATPase from Bufo marinus has been used in immunostaining rat kidney and salivary glands. Intense staining was observed on basolateral membranes of distal tubules of the kidney and striated ducts of the three major salivary glands. Less intense staining was seen on the basolateral membranes of parotid acinar cells, but no staining was seen on the acinar cells of submandibular or sublingual glands. These sites of staining have been shown, by other methods, to posses substantial Na+, K+ -ATPase, indicating that the antibody recognizes antigenic determinants of the sodium pump highly conserved in the course of evolution. In addition, staining with this antibody was observed at the apical region of cells of the proximal straight tubule and of the papillary collecting duct in the kidney. Absorption studies suggest that the apical antigenic determinants are the same or closely related to each other but are distinct from basolateral antigenic determinants.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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