ALBERT

Beschreibung: by Xierong Wei, Amanda J. Smith, David W. Forrest, Gabriel A. Cardenas, Dano W. Beck, Marlene LaLota, Lisa R. Metsch, Catlainn Sionean, S. Michele Owen, Jeffrey A. Johnson Objective To assess the utility of cost-effective dried blood spot (DBS) field sampling for incidence and drug resistance surveillance of persons at high risk for HIV infection. Methods We evaluated DBS collected in 2007–2010 in non-clinical settings by finger-stick from HIV-positive heterosexuals at increased risk of HIV infection (n = 124), men who have sex with men (MSM, n = 110), and persons who inject drugs (PWID, n = 58). Relative proportions of recent-infection findings among risk groups were assessed at avidity index (AI) cutoffs of ≤25%, ≤30%, and ≤35%, corresponding to an infection mean duration of recency (MDR) of 220.6, 250.4, and 278.3 days, respectively. Drug resistance mutation prevalence was compared among the risk groups and avidity indices. Results HIV antibody avidity testing of all self-reported ARV-naïve persons (n = 186) resulted in 9.7%, 11.3% and 14.0% with findings within the 221, 250, and 278-day MDRs, respectively. The proportion of ARV-naïve MSM, heterosexuals, and PWID reporting only one risk category who had findings below the suggested 30% AI was 23.1%, 6.9% and 3.6% (p

Beschreibung: by Li Zhang, Lihua Wu, Feng Tian, Zheng Wang A large amount of medical waste is produced during disaster relief, posing a potential hazard to the habitat and the environment. A comprehensive understanding of the composition and characteristics of medical waste that requires management is one of the most basic steps in the development of a plan for medical waste management. Unfortunately, limited reliable information is available in the open literature on the characteristics of the medical waste that is generated at disaster relief sites. This paper discusses the analysis of the composition and characteristics of medical waste at a disaster relief site using the retrospection-simulation-revision method. For this study, we obtained 35 medical relief records of the Wenchuan Earthquake, Sichuan, May 2008 from a field cabin hospital. We first present a retrospective analysis of the relief medical records, and then, we simulate the medical waste generated in the affected areas. We ultimately determine the composition and characteristics of medical waste in the affected areas using untreated medical waste to revise the composition of the simulated medical waste. The results from 35 cases showed that the medical waste generated from disaster relief consists of the following: plastic (43.2%), biomass (26.3%), synthetic fiber (15.3%), rubber (6.6%), liquid (6.6%), inorganic salts (0.3%) and metals (1.7%). The bulk density of medical relief waste is 249 kg/m 3 , and the moisture content is 44.75%. The data should be provided to assist the collection, segregation, storage, transportation, disposal and contamination control of medical waste in affected areas. In this paper, we wish to introduce this research method of restoring the medical waste generated in disaster relief to readers and researchers. In addition, we hope more disaster relief agencies will become aware of the significance of medical case recording and storing. This may be very important for the environmental evaluation of medical waste in disaster areas, as well as for medical waste management and disposal.

Beschreibung: Evaluation of IWV from the numerical weather prediction WRF model with PPP GNSS processing for Bulgaria Tzvetan Simeonov, Dmitry Sidorov, Felix Norman Teferle, Georgi Milev, and Guergana Guerova Atmos. Meas. Tech. Discuss., doi:10.5194/amt-2016-152,2016 Manuscript under review for AMT (discussion: open, 0 comments) Global Navigation Satellite Systems (GNSS) meteorology is an established operational service providing hourly updated GNSS tropospheric products to the National Meteorologic Services (NMS) in Europe. In the last decade through the ground-based GNSS network densification and new processing strategies like Precise Point Positioning (PPP) it has become possible to obtain sub-hourly tropospheric products for monitoring severe weather events. In this work one year (January–December 2013) of sub-hourly GNSS tropospheric products (Zenith Total Delay) are computed using the PPP strategy for seven stations in Bulgaria. In order to take advantage of the sub-hourly GNSS data to derive Integrated Water Vapour (IWV) surface pressure and temperature with similar temporal resolution is required. As the surface observations are on 3 hourly basis the first step is to compare the surface pressure and temperature from numerical weather prediction model Weather Forecasting and Research (WRF) with observations at three synoptic stations in Bulgaria. The mean difference between the two data-sets for 1) surface pressure is less than 0.5 hPa and the correlation is over 0.989, 2) temperature the largest mean difference is 1.1 °C and the correlation coefficient is over 0.957 and 3) IWV mean difference is in range 0.1–1.1 mm. The evaluation of WRF on annual bases shows IWV underestimation between 0.5 and 1.5 mm at five stations and overestimation at Varna and Rozhen. Varna and Rozhen have also much smaller correlation 0.9 and 0.76. The study of the monthly IWV variation shows that at those locations the GNSS IWV has unexpected drop in April and March respectively. The reason for this drop is likely problems with station raw data. At the remaining 5 stations a very good agreement between GNSS and WRF is observed with high correlation during the cold part of 2013 i.e. March, October and December (0.95) and low correlation during the warm part of 2013 i.e. April to August (below 0.9). The diurnal cycle of the WRF model shows a dry bias in the range of 0.5-1.5 mm. Between 00 and 01 UTC the GNSS IWV tends to be underestimate IWV which is likely due to the processing window used. The precipitation efficiency from GNSS and WRF show very good agreement on monthly bases with a maximum in May-June and minimum in August–September. The annual precipitation efficiency in 2013 at Lovech and Burgas is about 6 %.

Beschreibung: by Jan Skoda, Marketa Hermanova, Tomas Loja, Pavel Nemec, Jakub Neradil, Petr Karasek, Renata Veselska Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Its dismal prognosis is often attributed to the presence of cancer stem cells (CSCs) that have been identified in PDAC using various markers. However, the co-expression of all of these markers has not yet been evaluated. Furthermore, studies that compare the expression levels of CSC markers in PDAC tumor samples and in cell lines derived directly from those tumors are lacking. Here, we analyzed the expression of putative CSC markers—CD24, CD44, epithelial cell adhesion molecule (EpCAM), CD133, and nestin—by immunofluorescence, flow cytometry and quantitative PCR in 3 PDAC-derived cell lines and by immunohistochemistry in 3 corresponding tumor samples. We showed high expression of the examined CSC markers among all of the cell lines and tumor samples, with the exception of CD24 and CD44, which were enriched under in vitro conditions compared with tumor tissues. The proportions of cells positive for the remaining markers were comparable to those detected in the corresponding tumors. Co-expression analysis using flow cytometry revealed that CD24 + /CD44 + /EpCAM + /CD133 + cells represented a significant population of the cells (range, 43 to 72%) among the cell lines. The highest proportion of CD24 + /CD44 + /EpCAM + /CD133 + cells was detected in the cell line derived from the tumor of a patient with the shortest survival. Using gene expression profiling, we further identified the specific pro-tumorigenic expression profile of this cell line compared with the profiles of the other two cell lines. Together, CD24 + /CD44 + /EpCAM + /CD133 + cells are present in PDAC cell lines derived from primary tumors, and their increased proportion corresponds with a pro-tumorigenic gene expression profile.

Beschreibung: by Tarcísio Visintin da Silva Galdino, Sunil Kumar, Leonardo S. S. Oliveira, Acelino C. Alfenas, Lisa G. Neven, Abdullah M. Al-Sadi, Marcelo C. Picanço The Mango Sudden Decline (MSD), also referred to as Mango Wilt, is an important disease of mango in Brazil, Oman and Pakistan. This fungus is mainly disseminated by the mango bark beetle, Hypocryphalus mangiferae (Stebbing), by infected plant material, and the infested soils where it is able to survive for long periods. The best way to avoid losses due to MSD is to prevent its establishment in mango production areas. Our objectives in this study were to: (1) predict the global potential distribution of MSD, (2) identify the mango growing areas that are under potential risk of MSD establishment, and (3) identify climatic factors associated with MSD distribution. Occurrence records were collected from Brazil, Oman and Pakistan where the disease is currently known to occur in mango. We used the correlative maximum entropy based model (MaxEnt) algorithm to assess the global potential distribution of MSD. The MaxEnt model predicted suitable areas in countries where the disease does not already occur in mango, but where mango is grown. Among these areas are the largest mango producers in the world including India, China, Thailand, Indonesia, and Mexico. The mean annual temperature, precipitation of coldest quarter, precipitation seasonality, and precipitation of driest month variables contributed most to the potential distribution of MSD disease. The mango bark beetle vector is known to occur beyond the locations where MSD currently exists and where the model predicted suitable areas, thus showing a high likelihood for disease establishment in areas predicted by our model. Our study is the first to map the potential risk of MSD establishment on a global scale. This information can be used in designing strategies to prevent introduction and establishment of MSD disease, and in preparation of efficient pest risk assessments and monitoring programs.

Beschreibung: by Ge Song, Xiao-Xia Shao, Qing-Ping Wu, Zeng-Guang Xu, Ya-Li Liu, Zhan-Yun Guo We recently developed novel bioluminescent binding assays for several protein/peptide hormones to study their interactions with receptors using the so far brightest NanoLuc reporter. To validate the novel bioluminescent binding assay using a variety of protein/peptide hormones, in the present work we applied it to the fibroblast growth factor (FGF) family using the prototype member FGF2 as an example. A fully active recombinant FGF2 retaining a unique exposed cysteine (Cys) residue was chemically conjugated with an engineered NanoLuc carrying a unique exposed Cys residue at the C-terminus via formation of an intermolecular disulfide linkage. The NanoLuc-conjugated FGF2 (FGF2-Luc) retained high binding affinity to the overexpressed FGFR1 and the endogenous FGF receptor with the calculated dissociation constants of 161 ± 21 pM ( n = 3) and 25 ± 4 pM ( n = 3), respectively. In competition binding assays using FGF2-Luc as a tracer, receptor-binding potencies of wild-type or mutant FGF2s were accurately quantified. Thus, FGF2-Luc represents a novel non-radioactive tracer for the quantitative measurement of ligand–receptor interactions in the FGF family. These data suggest that the novel bioluminescent binding assay can be applied to a variety of protein/peptide hormones for ligand–receptor interaction studies.

Beschreibung: Contrasting radiation and soil heat fluxes in Arctic shrub and wet sedge tundra Inge Juszak, Werner Eugster, Monique M. P. D. Heijmans, and Gabriela Schaepman-Strub Biogeosciences, 13, 4049-4064, doi:10.1594/PANGAEA.860561, 2016 Changes in Arctic vegetation composition and structure feed back to climate and permafrost. Using field observations at a Siberian tundra site, we find that dwarf shrubs absorb more solar radiation than wet sedges and thus amplify surface warming, especially during snow melt. On the other hand, permafrost thaw was enhanced below sedges as a consequence of high soil moisture. Standing dead sedge leaves affected the radiation budget strongly and deserve more scientific attention.

Beschreibung: Holocene hydrological changes in the Rhône River (NW Mediterranean) as recorded in the marine mud belt Maria-Angela Bassetti, Serge Berné, Marie-Alexandrine Sicre, Bernard Dennielou, Yoann Alonso, Roselyne Buscail, Bassem Jalali, Bertil Hebert, and Christophe Menniti Clim. Past, 12, 1539-1553, doi:10.5194/cp-12-1539-2016, 2016 This work represents the first attempt to decipher the linkages between rapid climate changes and continental Holocene paleohydrology in the NW Mediterranean shallow marine setting. Between 11 and 4 ka cal BP, terrigenous input increased and reached a maximum at 7 ka cal BP, probably as a result of a humid phase. From ca. 4 ka cal BP to the present, enhanced variability in the land-derived material is possibly due to large-scale atmospheric circulation and rainfall patterns in western Europe.

Beschreibung: Effects of Multiple Doppler Radar data assimilation on the numerical simulation of a Flash Flood Event during the HyMeX campaign Ida Maiello, Sabrina Gentile, Rossella Ferretti, Luca Baldini, Nicoletta Roberto, Errico Picciotti, Pier Paolo Alberoni, and Frank S. Marzano Hydrol. Earth Syst. Sci. Discuss., doi:10.5194/hess-2016-320,2016 Manuscript under review for HESS (discussion: open, 0 comments) An analysis to evaluate the impact of assimilating multiple radar data with a three dimensional variational (3D-Var) system on a heavy precipitation event is presented. The main goal is to establish a general methodology to quantitatively assess the performance of flash-flood numerical weather prediction at mesoscale. In this respect, during the first Special Observation Period (SOP1) of HyMeX (Hydrological cycle in the Mediterranean Experiment) campaign several Intensive Observing Periods (IOPs) were launched and nine occurred in Italy. Among them IOP4 is chosen for this study because of its low predictability. This event hit central Italy on 14 September 2012 producing heavy precipitation and causing several damages. Data taken from three C-band radars running operationally during the event are assimilated to improve high resolution initial conditions. In order to evaluate the impact of the assimilation procedure at different horizontal resolution and to assess the impact of assimilating multiple radars data, several experiments using Weather Research and Forecasting (WRF) model are performed. Finally, the statistical indexes as accuracy, equitable threat score, false alarm ratio and frequency bias are used to objectively compare the experiments, using rain gauges data as benchmark.

Beschreibung: Model-based study of the role of rainfall and land use–land cover in the changes in the occurrence and intensity of Niger red floods in Niamey between 1953 and 2012 Claire Casse, Marielle Gosset, Théo Vischel, Guillaume Quantin, and Bachir Alkali Tanimoun Hydrol. Earth Syst. Sci., 20, 2841-2859, doi:10.5194/hess-20-2841-2016, 2016 Since 1950, the Niger River basin has overcome drastic changes. In Niamey city, the highest river levels and the longest flooded period ever recorded occurred in 2003, 2010, 2012 and 2013, with heavy casualties and property damage. The reasons for these changes, and the relative role of climate versus Land Use Land Cover changes are still debated and are investigated in this paper using observations and modelling. We conclude on the successive role of cover and then rainfall variability.

Beschreibung: Upscaling instantaneous to daily evapotranspiration using modelled daily shortwave radiation for remote sensing applications: an Artificial Neural Network approach Loise Wandera, Kaninska Mallick, Gerard Kiely, Olivier Roupsard, Matthias Peichl, and Vicenzo Magliulo Hydrol. Earth Syst. Sci. Discuss., doi:10.5194/hess-2016-344,2016 Manuscript under review for HESS (discussion: open, 0 comments) Upscaling instantaneous to daily evapotranspiration (ET i to ET d ) is one of the central challenge in regional vegetation water use mapping using polar orbiting satellites. Here we developed a robust ET i upscaling for global studies using the ratio between daily and instantaneous global radiation (R Sd /R Si ). Using data from 126 FLUXNET tower sites this study demonstrated R Sd /R si ratio to be the most robust factor explaining ET d /ET i variability across variable sky conditions and multiple biomes.

Beschreibung: by Isabel C. Caballero, John M. Bates, Mary Hennen, Mary V. Ashley Peregrine falcons ( Falco peregrinus ) were extirpated from most of the continental United States by widespread use of the pesticide DDT in the 1960s. Populations have rebounded with banning of the pesticide and successful implementation of captive breeding and hacking programs. An essentially new population of Midwestern peregrines now exists that is comprised almost entirely of urban-nesting birds. The new population is considered to be of mixed ancestry, occurs at relatively high densities, and has nest sites in close proximity, factors that could influence breeding behaviors including mate fidelity, nest-site fidelity, extra-pair paternity, and natal dispersal. We investigated these behaviors using a combination of field observations and DNA microsatellite genotyping. Data for eleven microsatellite DNA markers, including eight newly developed for the species, were analyzed from a total of 350 birds from nine Midwestern cities, representing 149 broods collected at 20 nest sites. To document breeding behavior, parentage was inferred by likelihood techniques when both parents were sampled and by parental genotype reconstruction when only one parent was sampled. In cases where neither parent was sampled, a sibship reconstruction approach was used. We found high mate fidelity and nest-site fidelity in urban peregrines; in 122 nesting attempts made by long-term breeders, only 12 (9.8%) mate changes and six (4.9%) nest-site changes occurred. Only one brood (of 35 tested) revealed extra-pair paternity and involved a male tending two offspring of a recently acquired mate. Natal dispersal patterns indicated that female peregrines dispersed on average 226 km, almost twice the distance of males (average 124 km). Despite the novel environment of cities, our results suggest that monogamous breeding, nest fidelity, and female natal dispersal are high in urban peregrines, not unlike other raptors living in non-urban habitats.

Beschreibung: by Henrik H. Hansen, Katrine Fabricius, Pernille Barkholt, Pernille Kongsbak-Wismann, Chantal Schlumberger, Jacob Jelsing, Dick Terwel, Annelies Termont, Charles Pyke, Lotte Bjerre Knudsen, Niels Vrang One of the major histopathological hallmarks of Alzheimer’s disease (AD) is cerebral deposits of extracellular β-amyloid peptides. Preclinical studies have pointed to glucagon-like peptide 1 (GLP-1) receptors as a potential novel target in the treatment of AD. GLP-1 receptor agonists, including exendin-4 and liraglutide, have been shown to promote plaque-lowering and mnemonic effects of in a number of experimental models of AD. Transgenic mouse models carrying genetic mutations of amyloid protein precursor (APP) and presenilin-1 (PS1) are commonly used to assess the pharmacodynamics of potential amyloidosis-lowering and pro-cognitive compounds. In this study, effects of long-term liraglutide treatment were therefore determined in two double APP/PS1 transgenic mouse models of Alzheimer’s disease carrying different clinical APP/PS1 mutations, i . e . the ‘London’ (hAPP Lon/ PS1 A246E ) and ‘Swedish’ mutation variant (hAPP Swe /PS1 ΔE9 ) of APP, with co-expression of distinct PS1 variants. Liraglutide was administered in 5 month-old hAPP Lon/ PS1 A246E mice for 3 months (100 or 500 ng/kg/day, s.c.), or 7 month-old hAPP Swe /PS1 ΔE9 mice for 5 months (500 ng/kg/day, s.c.). In both models, regional plaque load was quantified throughout the brain using stereological methods. Vehicle-dosed hAPP Swe /PS1 ΔE9 mice exhibited considerably higher cerebral plaque load than hAPP Lon/ PS1 A246E control mice. Compared to vehicle-dosed transgenic controls, liraglutide treatment had no effect on the plaque levels in hAPP Lon /PS1 A246E and hAPP Swe /PS1 ΔE9 mice. In conclusion, long-term liraglutide treatment exhibited no effect on cerebral plaque load in two transgenic mouse models of low- and high-grade amyloidosis, which suggests differential sensitivity to long-term liraglutide treatment in various transgenic mouse models mimicking distinct pathological hallmarks of AD.

Beschreibung: by Ye Fang, Yun Ding, Wei P. Feinstein, David M. Koppelman, Juana Moreno, Mark Jarrell, J. Ramanujam, Michal Brylinski Computational modeling of drug binding to proteins is an integral component of direct drug design. Particularly, structure-based virtual screening is often used to perform large-scale modeling of putative associations between small organic molecules and their pharmacologically relevant protein targets. Because of a large number of drug candidates to be evaluated, an accurate and fast docking engine is a critical element of virtual screening. Consequently, highly optimized docking codes are of paramount importance for the effectiveness of virtual screening methods. In this communication, we describe the implementation, tuning and performance characteristics of GeauxDock, a recently developed molecular docking program. GeauxDock is built upon the Monte Carlo algorithm and features a novel scoring function combining physics-based energy terms with statistical and knowledge-based potentials. Developed specifically for heterogeneous computing platforms, the current version of GeauxDock can be deployed on modern, multi-core Central Processing Units (CPUs) as well as massively parallel accelerators, Intel Xeon Phi and NVIDIA Graphics Processing Unit (GPU). First, we carried out a thorough performance tuning of the high-level framework and the docking kernel to produce a fast serial code, which was then ported to shared-memory multi-core CPUs yielding a near-ideal scaling. Further, using Xeon Phi gives 1.9× performance improvement over a dual 10-core Xeon CPU, whereas the best GPU accelerator, GeForce GTX 980, achieves a speedup as high as 3.5×. On that account, GeauxDock can take advantage of modern heterogeneous architectures to considerably accelerate structure-based virtual screening applications. GeauxDock is open-sourced and publicly available at www.brylinski.org/geauxdock and https://figshare.com/articles/geauxdock_tar_gz/3205249.

Beschreibung: by Rinske Loeffen, René van Oerle, Mathie P. G. Leers, Johannes A. Kragten, Harry Crijns, Henri M. H. Spronk, Hugo ten Cate Objective In acute coronary syndrome (ACS) cardiac cell damage is preceded by thrombosis. Therefore, plasma coagulation markers may have additional diagnostic relevance in ACS. By using novel coagulation assays this study aims to gain more insight into the relationship between the coagulation system and ACS. Methods We measured plasma thrombin generation, factor XIa and D-dimer levels in plasma from ACS (n = 104) and non-ACS patients (n = 42). Follow-up measurements (n = 73) were performed at 1 and 6 months. Associations between coagulation markers and recurrent cardiovascular events were calculated by logistic regression analysis. Results Thrombin generation was significantly enhanced in ACS compared to non-ACS patients: peak height 148±53 vs. 122±42 nM. There was a significantly diminished ETP reduction (32 vs. 41%) and increased intrinsic coagulation activation (25 vs. 7%) in ACS compared to non-ACS patients. Furthermore, compared to non-ACS patients factor XIa and D-dimer levels were significantly elevated in ACS patients: 1.9±1.1 vs. 1.4±0.7 pM and 495(310–885) vs. 380(235–540) μg/L. Within the ACS spectrum, ST-elevated myocardial infarction patients had the highest prothrombotic profile. During the acute event, thrombin generation was significantly increased compared to 1 and 6 months afterwards: peak height 145±52 vs. 100±44 vs. 98±33 nM. Both peak height and factor XIa levels on admission predicted recurrent cardiovascular events (OR: 4.9 [95%CI 1.2–20.9] and 4.5 [1.1–18.9]). Conclusion ACS patients had an enhanced prothrombotic profile, demonstrated by an increased thrombin generation potential, factor XIa and D-dimer levels. This study is the first to demonstrate the positive association between factor XIa, thrombin generation and recurrent cardiovascular events.

Beschreibung: 4-D imaging of sub-second dynamics in pore-scale processes using real-time synchrotron X-ray tomography Katherine J. Dobson, Sophia B. Coban, Samuel A. McDonald, Joanna N. Walsh, Robert C. Atwood, and Philip J. Withers Solid Earth, 7, 1059-1073, doi:10.5194/se-7-1059-2016, 2016 State-of-the-art synchrotron x-ray imaging was used to observe micron scale transport processes in real time. The 20 Hz 3-D image acquisition rates give experimental data free from motion artefacts, and suitable for detailed quantitative analysis of the dynamic fluid distribution, flow pathways and processes. The method marks a major breakthrough in our ability to capture both sub-second and lower frequency non-equilibrium process in many geological or engineering systems.

Beschreibung: Alpine tectonic wedging and crustal delamination in the Cantabrian Mountains (NW Spain) Jorge Gallastegui, Javier A. Pulgar, and Josep Gallart Solid Earth, 7, 1043-1057, doi:10.5194/se-7-1043-2016, 2016 We present the interpretation of deep seismic reflection profile ESCIN-2 across the boundary between the Cantabrian Mountains and the Cenozoic Duero Basin (NW Spain). 2-D seismic modelling allowed us to construct a N-S geological cross section which is compatible with the rest of the geophysical data. This profile shows the importance of the N-S Alpine deformation. A wedge of Cantabrian margin crust indented southwards into the delaminated Iberian crust, forcing its northward subduction.

Beschreibung: by Kristen R. Yeung, Christine L. Chiu, Suzanne Pears, Scott J. Heffernan, Angela Makris, Annemarie Hennessy, Joanne M. Lind Background Ageing is associated with changes at the molecular and cellular level that can alter cardiovascular function and ultimately lead to disease. The baboon is an ideal model for studying ageing due to the similarities in genetic, anatomical, physiological and biochemical characteristics with humans. The aim of this cross-sectional study was to investigate the changes in cardiovascular profile of baboons over the course of their lifespan. Methods Data were collected from 109 healthy baboons ( Papio hamadryas ) at the Australian National Baboon Colony. A linear regression model, adjusting for sex, was used to analyse the association between age and markers of ageing with P 〈 0.01 considered significant. Results Male (n = 49, 1.5–28.5 years) and female (n = 60, 1.8–24.6 years) baboons were included in the study. Age was significantly correlated with systolic (R 2 = 0.23, P 〈 0.001) and diastolic blood pressure (R 2 = 0.44, P 〈 0.001), with blood pressure increasing with age. Age was also highly correlated with core augmentation index (R 2 = 0.17, P 〈 0.001) and core pulse pressure (R 2 = 0.30, P 〈 0.001). Creatinine and urea were significantly higher in older animals compared to young animals (P 〈 0.001 for both). Older animals (〉12 years) had significantly shorter telomeres when compared to younger (

Beschreibung: Studying local earthquakes in the area Baltic-Bothnia Megashear using the data of the POLENET/LAPNET temporary array Olga Usoltseva and Elena Kozlovskaya Solid Earth, 7, 1095-1108, doi:10.5194/se-7-1095-2016, 2016 We address the problem of seismicity in the intraplate area of northern Fennoscandia using the information on local events recorded by the POLENET/LAPNET seismic array. We relocate the seismic events, calculate a 3-D tomographic P wave velocity model of the uppermost crust and investigate a focal mechanism of the event. Our results demonstrate that the Baltic-Bothnia Megashear is an important large-scale, reactivated tectonic structure that has to be taken into account when estimating seismic hazard.

Beschreibung: Studying local earthquakes in the area Baltic-Bothnia Megashear using the data of the POLENET/LAPNET temporary array Olga Usoltseva and Elena Kozlovskaya Solid Earth, 7, 1095-1108, doi:10.5194/se-7-1095-2016, 2016 We address the problem of seismicity in the intraplate area of northern Fennoscandia using the information on local events recorded by the POLENET/LAPNET seismic array. We relocate the seismic events, calculate a 3-D tomographic P wave velocity model of the uppermost crust and investigate a focal mechanism of the event. Our results demonstrate that the Baltic-Bothnia Megashear is an important large-scale, reactivated tectonic structure that has to be taken into account when estimating seismic hazard.

Beschreibung: Combined deep sampling and mass-based approaches to assess soil carbon and nitrogen losses due to land-use changes in karst area of southwestern China Yecui Hu, Zhangliu Du, Qibing Wang, and Guichun Li Solid Earth, 7, 1075-1084, doi:10.5194/se-7-1075-2016, 2016 We quantified the SOC and TN losses induced by land-use changes in a typical karst region of southwestern China. Converting from natural forest to croplands greatly led to SOC and TN losses (as higher as 37.8 %), but the magnitude depended on sampling depth and calculation method. We recommend to account for SOC and TN stocks on equivalent soil mass basis together with deep sampling. This study has significant implications on the projected land management in the degraded karst areas.

Beschreibung: Experimental sand burial affects seedling survivorship, morphological traits, and biomass allocation of Ulmus pumila var. sabulosa in the Horqin Sandy Land, China Jiao Tang, Carlos Alberto Busso, Deming Jiang, Ala Musa, Dafu Wu, Yongcui Wang, and Chunping Miao Solid Earth, 7, 1085-1094, doi:10.5194/se-7-1085-2016, 2016 In order to test the tolerance of sandy elm after emergence, an experiment was conducted to evaluate the changes of survivorship, morphological traits and biomass allocation buried by various experimental burial depths. The results showed that partial sand burial did not influence survivorship but increased seedling height, diameter, taproot length, total biomass, and relative growth rates. It decreased total leaf area and changed biomass allocation, transferring more biomass to aboveground.

Beschreibung: Characteristics of an avalanche-feeding and partially debris-covered glacier and its response to atmospheric warming in Mt. Tomor, Tian Shan, China Puyu Wang, Zhongqin Li, and Huilin Li The Cryosphere Discuss., doi:10.5194/tc-2016-138,2016 Manuscript under review for TC (discussion: open, 0 comments) A cirque-valley glacier with complex topography and partially debris-covered area was investigated in the Mt. Tomor, Tian Shan. The glacier is analogous to temperate one on movement and temperature regimes. The strongest ablation and most significant terminus retreat and area reduction occurred at the end of last century and the beginning of this century. Since inhibition of debris cover to melting, it is expected to keep shrinkage in the coming decades, but the terminus retreat is to be slower.

Beschreibung: On the temporal variability of the surface solar radiation by means of spectral representations Marc Bengulescu, Philippe Blanc, and Lucien Wald Adv. Sci. Res., 13, 121-127, doi:10.5194/asr-13-121-2016, 2016 The continuous wavelet (CWT) and the Hilbert–Huang transforms (HHT) are compared for the analysis of the temporal variability on ten years of daily means of the surface solar irradiance. In both cases, the variability exhibits a plateau between scales of two days and three months that has decreasing power with increasing scale, a spectral peak corresponding to the annual cycle, and a low power regime in-between. The HHT is shown to be suitable for inspecting the variability of the measurements.

Beschreibung: Development of high-resolution multi-scale modelling system for simulation of coastal-fluvial urban flooding Agnieszka Indiana Olbert, Joanne Comer, Stephen Nash, and Michael Hartnett Nat. Hazards Earth Syst. Sci. Discuss., doi:10.5194/nhess-2016-238,2016 Manuscript under review for NHESS (discussion: open, 0 comments) A novel nested flood model (MSN_Flood) is applied to simulate complex coastal-fluvial urban flooding in order to critically examine the model's capability to forecast evolution of urban inundation. The model demonstrates high accuracy of outputs without incurring the computational expense of high spatial resolution over the entire model domain. MSN_Flood provides full characteristics of water levels and flow regimes necessary for flood hazard identification and flood risk assessment.

Beschreibung: Development of high-resolution multi-scale modelling system for simulation of coastal-fluvial urban flooding Agnieszka Indiana Olbert, Joanne Comer, Stephen Nash, and Michael Hartnett Nat. Hazards Earth Syst. Sci. Discuss., doi:10.5194/nhess-2016-238,2016 Manuscript under review for NHESS (discussion: open, 0 comments) A novel nested flood model (MSN_Flood) is applied to simulate complex coastal-fluvial urban flooding in order to critically examine the model's capability to forecast evolution of urban inundation. The model demonstrates high accuracy of outputs without incurring the computational expense of high spatial resolution over the entire model domain. MSN_Flood provides full characteristics of water levels and flow regimes necessary for flood hazard identification and flood risk assessment.

Beschreibung: Turbulence kinetic energy budget during the afternoon transition – Part 2: A simple TKE model Erik Nilsson, Marie Lothon, Fabienne Lohou, Eric Pardyjak, Oscar Hartogensis, and Clara Darbieu Atmos. Chem. Phys., 16, 8873-8898, doi:10.5194/acp-16-8873-2016, 2016 A new simple model for turbulence kinetic energy (TKE) and its budget is presented for the sheared convective atmospheric boundary layer. It is used to study effects of buoyancy and shear on TKE evolution during the afternoon transition, especially near the surface. We also find a region of weak turbulence during unstable afternoon conditions below the inversion top, which we refer to as a "pre-residual layer".

Beschreibung: Turbulence kinetic energy budget during the afternoon transition – Part 1: Observed surface TKE budget and boundary layer description for 10 intensive observation period days Erik Nilsson, Fabienne Lohou, Marie Lothon, Eric Pardyjak, Larry Mahrt, and Clara Darbieu Atmos. Chem. Phys., 16, 8849-8872, doi:10.5194/acp-16-8849-2016, 2016 The evolution of near-surface turbulence kinetic energy (TKE) and its budget in the afternoon transition has been studied based on field measurements. The study shows that TKE transport is an important budget term that needs to be taken into account in modeling of TKE. A non-local parametrization of dissipation using a TKE–length scale model which takes into account of boundary layer depth also gave improved results compared to a local parametrization.

Beschreibung: Ice nucleation efficiency of AgI: review and new insights Claudia Marcolli, Baban Nagare, André Welti, and Ulrike Lohmann Atmos. Chem. Phys., 16, 8915-8937, doi:10.5194/acp-16-8915-2016, 2016 Silver iodide is one of the best-investigated ice nuclei. It has relevance for the atmosphere since it is used for glaciogenic cloud seeding. Nevertheless, many open questions remain. This paper gives an overview of silver iodide as an ice nucleus and tries to identify the factors that influence the ice nucleation ability of silver iodide.

Beschreibung: A comprehensive biomass burning emission inventory with high spatial and temporal resolution in China Ying Zhou, Xiaofan Xing, Jianlei Lang, Dongsheng Chen, Shuiyuan Cheng, Lin Wei, Xiao Wei, and Chao Liu Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-560,2016 Manuscript under review for ACP (discussion: open, 0 comments) A 1-km gridded and comprehensive biomass burning emission inventory is developed for mainland China in 2012, based on the county-level activity data and updated source-specific emission factors. Several important parameters for emissions estimation are investigated. This paper presents the detailed emission inventory for air quality modelling and supports the development of a targeted control strategy for biomass pollution.

Beschreibung: How stratospheric are deep stratospheric intrusions? LUAMI 2008 Thomas Trickl, Hannes Vogelmann, Andreas Fix, Andreas Schäfler, Martin Wirth, Bertrand Calpini, Gilbert Levrat, Gonzague Romanens, Arnoud Apituley, Keith M. Wilson, Robert Begbie, Jens Reichardt, Holger Vömel, and Michael Sprenger Atmos. Chem. Phys., 16, 8791-8815, doi:10.5194/acp-16-8791-2016, 2016 A rather homogeneous deep stratospheric intrusion event was mapped by vertical sounding over central Europe and by model calculations along the transport path. The very low minimum H 2 O mixing ratios demonstrate almost negligible mixing with tropospheric air during the downward transport. The vertical distributions of O 3 and aerosol were transferred from the source region to Europe without major change. A rather shallow outflow from the stratosphere was found.

Beschreibung: Air–surface exchange of gaseous mercury over permafrost soil: an investigation at a high-altitude (4700 m a.s.l.) and remote site in the central Qinghai-Tibet Plateau Zhijia Ci, Fei Peng, Xian Xue, and Xiaoshan Zhang Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-515,2016 Manuscript under review for ACP (discussion: open, 0 comments) We performed filed measurements and controlled field experiments to examine the flux, temporal variation and influencing factors of air–surface Hg(0) exchange at a high-altitude and remote site in the central Qinghai-Tibet Plateau. We found that the environmental conditions greatly influenced the air–surface Hg dynamics. Our results have important implications to the Hg biogeochemical cycle in the soils of Qinghai-Tibet Plateau under the rapid climate warming and environmental change.

Beschreibung: Ice nucleation efficiency of AgI: review and new insights Claudia Marcolli, Baban Nagare, André Welti, and Ulrike Lohmann Atmos. Chem. Phys., 16, 8915-8937, doi:10.5194/acp-16-8915-2016, 2016 Silver iodide is one of the best-investigated ice nuclei. It has relevance for the atmosphere since it is used for glaciogenic cloud seeding. Nevertheless, many open questions remain. This paper gives an overview of silver iodide as an ice nucleus and tries to identify the factors that influence the ice nucleation ability of silver iodide.

Beschreibung: Controls on phase composition and ice water content in a convection-permitting model simulation of a tropical mesoscale convective system Charmaine N. Franklin, Alain Protat, Delphine Leroy, and Emmanuel Fontaine Atmos. Chem. Phys., 16, 8767-8789, doi:10.5194/acp-16-8767-2016, 2016 Simulations of tropical convection are used to investigate the processes that control the phase composition of tropical clouds. It is shown that the growth of ice is less dependent on vertical velocity than is liquid water, with the control on ice water content being the ice sizes and available liquid water. Results show that in the purely ice region of convective updraughts, the buoyancy is controlled by the ice sizes, demonstrating the importance of microphysical processes on convective dynamics.

Beschreibung: Relative humidity-dependent viscosity of secondary organic material from toluene photo-oxidation and possible implications for organic particulate matter over megacities Mijung Song, Pengfei F. Liu, Sarah J. Hanna, Rahul A. Zaveri, Katie Potter, Yuan You, Scot T. Martin, and Allan K. Bertram Atmos. Chem. Phys., 16, 8817-8830, doi:10.5194/acp-16-8817-2016, 2016 To improve predictions of air quality, visibility, and climate change, knowledge of the viscosities and diffusion rates within organic particulate matter consisting of secondary organic material (SOM) is required. Most qualitative and quantitative measurements of viscosity and diffusion rates within organic particulate matter have focused on SOM particles generated from biogenic volatile organic compounds (VOCs) such as α -pinene and isoprene. In this study, we quantify the relative humidity (RH)-dependent viscosities at 295 ± 1 K of SOM produced by photo-oxidation of toluene, an anthropogenic VOC. The viscosities of toluene-derived SOM were 2  ×  10 −1 to  ∼  6  ×  10 6  Pa s from 30 to 90 % RH, and greater than  ∼  2  ×  10 8  Pa s (similar to or greater than the viscosity of tar pitch) for RH  ≤  17 %. These viscosities correspond to Stokes–Einstein-equivalent diffusion coefficients for large organic molecules of  ∼  2  ×  10 −15  cm 2  s −1 for 30 % RH, and lower than  ∼  3  ×  10 −17  cm 2  s −1 for RH  ≤  17 %. Based on these estimated diffusion coefficients, the mixing time of large organic molecules within 200 nm toluene-derived SOM particles is 0.1–5 h for 30 % RH, and higher than  ∼  100 h for RH  ≤  17 %. As a starting point for understanding the mixing times of large organic molecules in organic particulate matter over cities, we applied the mixing times determined for toluene-derived SOM particles to the world's top 15 most populous megacities. If the organic particulate matter in these megacities is similar to the toluene-derived SOM in this study, in Istanbul, Tokyo, Shanghai, and São Paulo, mixing times in organic particulate matter during certain periods of the year may be very short, and the particles may be well-mixed. On the other hand, the mixing times of large organic molecules in organic particulate matter in Beijing, Mexico City, Cairo, and Karachi may be long and the particles may not be well-mixed in the afternoon (15:00–17:00 LT) during certain times of the year.

Beschreibung: Air–surface exchange of gaseous mercury over permafrost soil: an investigation at a high-altitude (4700 m a.s.l.) and remote site in the central Qinghai-Tibet Plateau Zhijia Ci, Fei Peng, Xian Xue, and Xiaoshan Zhang Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-515,2016 Manuscript under review for ACP (discussion: open, 0 comments) We performed filed measurements and controlled field experiments to examine the flux, temporal variation and influencing factors of air–surface Hg(0) exchange at a high-altitude and remote site in the central Qinghai-Tibet Plateau. We found that the environmental conditions greatly influenced the air–surface Hg dynamics. Our results have important implications to the Hg biogeochemical cycle in the soils of Qinghai-Tibet Plateau under the rapid climate warming and environmental change.

Beschreibung: Aerosol source apportionment from 1-year measurements at the CESAR tower in Cabauw, the Netherlands Patrick Schlag, Astrid Kiendler-Scharr, Marcus Johannes Blom, Francesco Canonaco, Jeroen Sebastiaan Henzing, Marcel Moerman, André Stephan Henry Prévôt, and Rupert Holzinger Atmos. Chem. Phys., 16, 8831-8847, doi:10.5194/acp-16-8831-2016, 2016 This work provides chemical composition data of atmospheric aerosols acquired during 1 year in the rural site of Cabauw, the Netherlands. In some periods, we found unexpected high particle mass concentrations exceeding the WHO limits. Using these composition data, we found that reducing ammonia emissions in this region would largely reduce the main aerosol component ammonium nitrate, whereas the local mitigation of the organics turned out to be difficult due to the lack of a designated source.

Beschreibung: Turbulence kinetic energy budget during the afternoon transition – Part 2: A simple TKE model Erik Nilsson, Marie Lothon, Fabienne Lohou, Eric Pardyjak, Oscar Hartogensis, and Clara Darbieu Atmos. Chem. Phys., 16, 8873-8898, doi:10.5194/acp-16-8873-2016, 2016 A new simple model for turbulence kinetic energy (TKE) and its budget is presented for the sheared convective atmospheric boundary layer. It is used to study effects of buoyancy and shear on TKE evolution during the afternoon transition, especially near the surface. We also find a region of weak turbulence during unstable afternoon conditions below the inversion top, which we refer to as a "pre-residual layer".

Beschreibung: Comparing contact and immersion freezing from continuous flow diffusion chambers Baban Nagare, Claudia Marcolli, André Welti, Olaf Stetzer, and Ulrike Lohmann Atmos. Chem. Phys., 16, 8899-8914, doi:10.5194/acp-16-8899-2016, 2016 The relative importance of contact freezing and immersion freezing at mixed-phase cloud temperatures is the subject of debate. We performed experiments using continuous-flow diffusion chambers to compare the freezing efficiency of ice-nucleating particles for both these nucleation modes. Silver iodide, kaolinite and Arizona Test Dust were used as ice-nucleating particles. We could not confirm the dominance of contact freezing over immersion freezing for our experimental conditions.

Beschreibung: Assessment of cloud related fine mode AOD enhancements based on AERONET SDA product A. Arola, T. F. Eck, H. Kokkola, M. R. A. Pitkänen, and S. Romakkaniemi Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-600,2016 Manuscript under review for ACP (discussion: open, 0 comments) One of the issues that hinder the measurement-based assessment of aerosol-cloud interactions by remote sensing methods is that typically aerosols and clouds cannot be measured simultaneously by passive remote sensing methods. AERONET includes so-called SDA product that provides the fine mode AOD also in mixed cloud-aerosol observations. These measurements have not been yet fully exploited in studies of aerosol-cloud interactions. We applied SDA for this kind of analysis.

Beschreibung: Controls on phase composition and ice water content in a convection-permitting model simulation of a tropical mesoscale convective system Charmaine N. Franklin, Alain Protat, Delphine Leroy, and Emmanuel Fontaine Atmos. Chem. Phys., 16, 8767-8789, doi:10.5194/acp-16-8767-2016, 2016 Simulations of tropical convection are used to investigate the processes that control the phase composition of tropical clouds. It is shown that the growth of ice is less dependent on vertical velocity than is liquid water, with the control on ice water content being the ice sizes and available liquid water. Results show that in the purely ice region of convective updraughts, the buoyancy is controlled by the ice sizes, demonstrating the importance of microphysical processes on convective dynamics.

Beschreibung: Turbulence kinetic energy budget during the afternoon transition – Part 1: Observed surface TKE budget and boundary layer description for 10 intensive observation period days Erik Nilsson, Fabienne Lohou, Marie Lothon, Eric Pardyjak, Larry Mahrt, and Clara Darbieu Atmos. Chem. Phys., 16, 8849-8872, doi:10.5194/acp-16-8849-2016, 2016 The evolution of near-surface turbulence kinetic energy (TKE) and its budget in the afternoon transition has been studied based on field measurements. The study shows that TKE transport is an important budget term that needs to be taken into account in modeling of TKE. A non-local parametrization of dissipation using a TKE–length scale model which takes into account of boundary layer depth also gave improved results compared to a local parametrization.

Beschreibung: Relative humidity-dependent viscosity of secondary organic material from toluene photo-oxidation and possible implications for organic particulate matter over megacities Mijung Song, Pengfei F. Liu, Sarah J. Hanna, Rahul A. Zaveri, Katie Potter, Yuan You, Scot T. Martin, and Allan K. Bertram Atmos. Chem. Phys., 16, 8817-8830, doi:10.5194/acp-16-8817-2016, 2016 To improve predictions of air quality, visibility, and climate change, knowledge of the viscosities and diffusion rates within organic particulate matter consisting of secondary organic material (SOM) is required. Most qualitative and quantitative measurements of viscosity and diffusion rates within organic particulate matter have focused on SOM particles generated from biogenic volatile organic compounds (VOCs) such as α -pinene and isoprene. In this study, we quantify the relative humidity (RH)-dependent viscosities at 295 ± 1 K of SOM produced by photo-oxidation of toluene, an anthropogenic VOC. The viscosities of toluene-derived SOM were 2  ×  10 −1 to  ∼  6  ×  10 6  Pa s from 30 to 90 % RH, and greater than  ∼  2  ×  10 8  Pa s (similar to or greater than the viscosity of tar pitch) for RH  ≤  17 %. These viscosities correspond to Stokes–Einstein-equivalent diffusion coefficients for large organic molecules of  ∼  2  ×  10 −15  cm 2  s −1 for 30 % RH, and lower than  ∼  3  ×  10 −17  cm 2  s −1 for RH  ≤  17 %. Based on these estimated diffusion coefficients, the mixing time of large organic molecules within 200 nm toluene-derived SOM particles is 0.1–5 h for 30 % RH, and higher than  ∼  100 h for RH  ≤  17 %. As a starting point for understanding the mixing times of large organic molecules in organic particulate matter over cities, we applied the mixing times determined for toluene-derived SOM particles to the world's top 15 most populous megacities. If the organic particulate matter in these megacities is similar to the toluene-derived SOM in this study, in Istanbul, Tokyo, Shanghai, and São Paulo, mixing times in organic particulate matter during certain periods of the year may be very short, and the particles may be well-mixed. On the other hand, the mixing times of large organic molecules in organic particulate matter in Beijing, Mexico City, Cairo, and Karachi may be long and the particles may not be well-mixed in the afternoon (15:00–17:00 LT) during certain times of the year.

Beschreibung: How stratospheric are deep stratospheric intrusions? LUAMI 2008 Thomas Trickl, Hannes Vogelmann, Andreas Fix, Andreas Schäfler, Martin Wirth, Bertrand Calpini, Gilbert Levrat, Gonzague Romanens, Arnoud Apituley, Keith M. Wilson, Robert Begbie, Jens Reichardt, Holger Vömel, and Michael Sprenger Atmos. Chem. Phys., 16, 8791-8815, doi:10.5194/acp-16-8791-2016, 2016 A rather homogeneous deep stratospheric intrusion event was mapped by vertical sounding over central Europe and by model calculations along the transport path. The very low minimum H 2 O mixing ratios demonstrate almost negligible mixing with tropospheric air during the downward transport. The vertical distributions of O 3 and aerosol were transferred from the source region to Europe without major change. A rather shallow outflow from the stratosphere was found.

Beschreibung: Publication date: 19 July 2016 Source: Cell Reports, Volume 16, Issue 3 Author(s): Sandra Malmgren Hill, Xinxin Hao, Johan Grönvall, Stephanie Spikings-Nordby, Per O. Widlund, Triana Amen, Anna Jörhov, Rebecca Josefson, Daniel Kaganovich, Beidong Liu, Thomas Nyström Age can be reset during mitosis in both yeast and stem cells to generate a young daughter cell from an aged and deteriorated one. This phenomenon requires asymmetry-generating genes (AGGs) that govern the asymmetrical inheritance of aggregated proteins. Using a genome-wide imaging screen to identify AGGs in Saccharomyces cerevisiae , we discovered a previously unknown role for endocytosis, vacuole fusion, and the myosin-dependent adaptor protein Vac17 in asymmetrical inheritance of misfolded proteins. Overproduction of Vac17 increases deposition of aggregates into cytoprotective vacuole-associated sites, counteracts age-related breakdown of endocytosis and vacuole integrity, and extends replicative lifespan. The link between damage asymmetry and vesicle trafficking can be explained by a direct interaction between aggregates and vesicles. We also show that the protein disaggregase Hsp104 interacts physically with endocytic vesicle-associated proteins, such as the dynamin-like protein, Vps1, which was also shown to be required for Vac17-dependent sequestration of protein aggregates. These data demonstrate that two physiognomies of aging—reduced endocytosis and protein aggregation—are interconnected and regulated by Vac17. Graphical abstract Teaser Cellular rejuvenation is enabled by asymmetrical inheritance of damaged proteins. Using a genome-wide imaging screen to identify asymmetry-generating genes, Hill et al. demonstrate a role for vesicle trafficking, membrane fusion, and the myosin-dependent adaptor protein Vac17 in the asymmetric inheritance of misfolded proteins and consequently in the regulation of lifespan.

Beschreibung: A low-cost acoustic permeameter Stephen A. Drake, John S. Selker, and Chad W. Higgins Geosci. Instrum. Method. Data Syst. Discuss., doi:10.5194/gi-2016-13,2016 Manuscript under review for GI (discussion: open, 0 comments) Intrinsic permeability of snow is an important parameter that regulates snow/atmosphere exchange. Current permeability measurements require specialized equipment to acquire in the field and have increased variability with increasing snow heterogeneity. To facilitate a field-based, volume-averaged measure of permeability we designed and assembled an acoustic permeameter. Using reticulated foam samples of known permeability, the mean relative error from known values was less than 20 %.

Beschreibung: Automatic georeferencing of astronaut auroral photography Maik Riechert, Andrew P. Walsh, Alexander Gerst, and Matthew G. G. T. Taylor Geosci. Instrum. Method. Data Syst., 5, 289-304, doi:10.5194/gi-5-289-2016, 2016 Astronauts on board the International Space Station have taken thousands of high-quality images of the northern and southern lights (aurorae). Because the images were not taken as part of a specific research project, no information about exactly where the camera was pointing was available. We have used the stars in the images to reconstruct this information. Now we can tell the latitudes and longitudes of the aurorae in the images and use them for research. The data are publicly available.

Beschreibung: On the propagation of diel signals in river networks using analytic solutions of flow equations Morgan Fonley, Ricardo Mantilla, Scott J. Small, and Rodica Curtu Hydrol. Earth Syst. Sci., 20, 2899-2912, doi:10.5194/hess-20-2899-2016, 2016 We design and implement a theoretical experiment to show that, under low-flow conditions, observed streamflow discrepancies between early and late summer can be attributed to different flow velocities in the river network. By developing an analytic solution to represent flow along a given river network, we emphasize the dependence of streamflow amplitude and time delay on the geomorphology of the network. We also simulate using a realistic river network to highlight the effects of scale.

Beschreibung: On the propagation of diel signals in river networks using analytic solutions of flow equations Morgan Fonley, Ricardo Mantilla, Scott J. Small, and Rodica Curtu Hydrol. Earth Syst. Sci., 20, 2899-2912, doi:10.5194/hess-20-2899-2016, 2016 We design and implement a theoretical experiment to show that, under low-flow conditions, observed streamflow discrepancies between early and late summer can be attributed to different flow velocities in the river network. By developing an analytic solution to represent flow along a given river network, we emphasize the dependence of streamflow amplitude and time delay on the geomorphology of the network. We also simulate using a realistic river network to highlight the effects of scale.

Beschreibung: Simultaneous calibration of hydrological models in geographical space András Bárdossy, Yingchun Huang, and Thorsten Wagener Hydrol. Earth Syst. Sci., 20, 2913-2928, doi:10.5194/hess-20-2913-2016, 2016 This paper explores the simultaneous calibration method to transfer model parameters from gauged to ungauged catchments. It is hypothesized that the model parameters can be separated into two categories: one reflecting the dynamic behavior and the other representing the long-term water balance. The results of three numerical experiments indicate that a good parameter transfer to ungauged catchments can be achieved through simultaneous calibration of models for a number of catchments.

Beschreibung: by Emanuela Jirayu Tanprasertsuk, Binxing Li, Paul S. Bernstein, Rohini Vishwanathan, Mary Ann Johnson, Leonard Poon, Elizabeth J. Johnson

Beschreibung: by Gertrud L. G. Haverkamp, Bart Torensma, Anton C. M. Vergouwen, Adriaan Honig

Beschreibung: by The PLOS ONE Staff

Beschreibung: by Hunter R. Underhill, Jacob O. Kitzman, Sabine Hellwig, Noah C. Welker, Riza Daza, Daniel N. Baker, Keith M. Gligorich, Robert C. Rostomily, Mary P. Bronner, Jay Shendure Malignant tumors shed DNA into the circulation. The transient half-life of circulating tumor DNA (ctDNA) may afford the opportunity to diagnose, monitor recurrence, and evaluate response to therapy solely through a non-invasive blood draw. However, detecting ctDNA against the normally occurring background of cell-free DNA derived from healthy cells has proven challenging, particularly in non-metastatic solid tumors. In this study, distinct differences in fragment length size between ctDNAs and normal cell-free DNA are defined. Human ctDNA in rat plasma derived from human glioblastoma multiforme stem-like cells in the rat brain and human hepatocellular carcinoma in the rat flank were found to have a shorter principal fragment length than the background rat cell-free DNA (134–144 bp vs. 167 bp, respectively). Subsequently, a similar shift in the fragment length of ctDNA in humans with melanoma and lung cancer was identified compared to healthy controls. Comparison of fragment lengths from cell-free DNA between a melanoma patient and healthy controls found that the BRAF V600E mutant allele occurred more commonly at a shorter fragment length than the fragment length of the wild-type allele (132–145 bp vs. 165 bp, respectively). Moreover, size-selecting for shorter cell-free DNA fragment lengths substantially increased the EGFR T790M mutant allele frequency in human lung cancer. These findings provide compelling evidence that experimental or bioinformatic isolation of a specific subset of fragment lengths from cell-free DNA may improve detection of ctDNA.

Beschreibung: by Sajid Javed, Leanne Marsay, Alice Wareham, Kuiama S. Lewandowski, Ann Williams, Michael J. Dennis, Sally Sharpe, Richard Vipond, Nigel Silman, Graham Ball, Karen E. Kempsell

Beschreibung: by Haiyou Deng, Quan Liu, Wei Cao, Rong Gui, Chengzhang Ma, Ming Yi, Yuangen Yao Recently, a new type of small interfering RNAs (qiRNAs) of typically 20~21 nucleotides was found in Neurospora crassa and rice and has been shown to regulate gene silencing in the DNA damage response. Identification of qiRNAs is fundamental for dissecting regulatory functions and molecular mechanisms. In contrast to other expensive and time-consuming experimental methods, the computational prediction of qiRNAs is a conveniently rapid method for gaining valuable information for a subsequent experimental verification. However, no tool existed to date for the prediction of qiRNAs. To this purpose, we developed the novel qiRNA prediction software package qiRNApredictor. This software demonstrates a promising sensitivity of 93.55% and a specificity of 71.61% from the leave-one-out validation. These studies might be beneficial for further experimental investigation. Furthermore, the local package of qiRNApredictor was implemented and made freely available to the academic community at Supplementary material.

Beschreibung: by Abhirami A. Ananth, Lee-Hwa Tai, Casey Lansdell, Almohanad A. Alkayyal, Katherine E. Baxter, Leonard Angka, Jiqing Zhang, Christiano Tanese de Souza, Kyle B Stephenson, Kelley Parato, Jonathan L Bramson, John C Bell, Brian D Lichty, Rebecca C Auer

Beschreibung: by Amanda N. Bolton Hall, Binoy Joseph, Jennifer M. Brelsfoard, Kathryn E. Saatman Millions of mild traumatic brain injuries (TBIs) occur every year in the United States, with many people subject to multiple head injuries that can lead to chronic behavioral dysfunction. We previously reported that mild TBI induced using closed head injuries (CHI) repeated at 24h intervals produced more acute neuron death and glial reactivity than a single CHI, and increasing the length of time between injuries to 48h reduced the cumulative acute effects of repeated CHI. To determine whether repeated CHI is associated with behavioral dysfunction or persistent cellular damage, mice receiving either five CHI at 24h intervals, five CHI at 48h intervals, or five sham injuries at 24h intervals were evaluated across a 10 week period after injury. Animals with repeated CHI exhibited motor coordination and memory deficits, but not gait abnormalities when compared to sham animals. At 10wks post-injury, no notable neuron loss or glial reactivity was observed in the cortex, hippocampus, or corpus callosum. Argyrophilic axons were found in the pyramidal tract of some injured animals, but neither silver stain accumulation nor inflammatory responses in the injury groups were statistically different from the sham group in this region. However, argyrophilic axons, microgliosis and astrogliosis were significantly increased within the optic tract of injured animals. Repeated mild CHI also resulted in microgliosis and a loss of neurofilament protein 200 in the optic nerve. Lengthening the inter-injury interval from 24h to 48h did not effectively reduce these behavioral or cellular responses. These results suggest that repeated mild CHI results in persistent behavioral dysfunction and chronic pathological changes within the visual system, neither of which was significantly attenuated by lengthening the inter-injury interval from 24h to 48h.

Beschreibung: by Hesung Now, Joo-Yeon Yoo A population often contains distinct sub-populations, thereby increasing the complexity of the overall heterogeneity. However, the cellular origin and biological relevance of sub-populations in cell population have not been clearly identified. Here we demonstrated the novel roles of ISGylation, which is an IFN-induced post-translational modification, controlling heterogeneity at the population level in cultured adherent cells. Without UBE1L, an E1 enzyme of ISGylation, mouse embryonic fibroblasts (MEF) exhibited low viral resistance despite high STAT1 and ISG expression compared with the wild-type MEF. We observe that Ube1l −/− MEF populations consist of two behaviorally distinguishable sub-populations with distinct basal STAT1 activity, while wild-type MEF populations are unimodal. This population heterogeneity in Ube1l knock-out cells was perturbed by tyrosine kinase inhibitors, AG490 and PF431396. In contrast, the neutralization of type I IFN did not affect population heterogeneity. Based on these results, we concluded that UBE1L functions to adjust basal immunological states with the regulation of population heterogeneity.

Beschreibung: by Silvia Wein, Birgit Beyer, Annika Gohlke, Ralf Blank, Cornelia C. Metges, Siegfried Wolffram Green tea catechins have various potential health benefits in humans including anti-inflammatory, anti-oxidative and hepato-protective effects. If present in the circulation, they might have similar effects in ruminants, which are exposed to oxidative stress and fatty liver disease such as dairy cows during the periparturient phase. However, the bioavailability of a substance is a prerequisite for any post absorptive effect in vivo . This study aimed to investigate the appearance of catechins from a green tea extract (GTE) in cattle plasma after intraruminal and intraduodenal administration because absorption is of major importance regarding the bioavailability of catechins. The studies were performed in 5 rumen-fistulated non-lactating heifers and 6 duodenally fistulated lactating dairy cows, respectively, equipped with indwelling catheters placed in a jugular vein. The GTE was applied intraruminally (10 and 50 mg/kg BW, heifers) or duodenally (10, 20 and 30 mg/kg BW, dairy cows) in a cross‐over design with a 2 d washout period between different dosages. Blood samples were drawn following the GTE administration at various pre-defined time intervals. The concentration of the major GTE catechins (gallocatechin, epigallocatechin, catechin, epicatechin, epigallocatechin-gallate, epicatechin-gallate) in plasma samples were analysed by HPLC with electrochemical detection. Irrespective of the dose, almost none of the catechins originally contained in the GTE were detected in plasma samples after intraruminal application. In contrast, intraduodenal administration of GTE resulted in increased plasma concentrations of epicatechin, epigallocatechin, epigallocatechin gallate in a dose‐dependent manner. Thus, we can conclude that intraruminally or orally administered catechins are intensively metabolized by ruminal microorganisms.

Beschreibung: by Haowen Tang, Bingmin Li, Aiqun Zhang, Wenping Lu, Canhong Xiang, Jiahong Dong Background and Objective Inflammation is deemed to play critical roles in tumor progression and metastasis, and an increased neutrophil-lymphocyte ratio (NLR) has been reported to correlate with poor survivals in various malignancies. However, association between NLR elevation and survival outcome in patients with colorectal liver metastasis (CRLM) remains controversial. The aim of this study was to investigate the prognostic significance of elevated NLR in CRLM. Methods The meta-analysis was conducted in adherence to the MOOSE guidelines. PubMed, Embase, Cochrane Library, Web of Science and the Chinese SinoMed were systematically searched to identify eligible studies from the initiation of the databases to May, 2016. Overall survival (OS) and recurrence free survival (RFS) were pooled by using hazard ratio (HR) with corresponding 95% confidence interval (CI). Correlation between NLR values and clinicopathological features was synthesized by using odds ratio (OR) with corresponding 95% CI. Results A total of 1685 patients from 8 studies (9 cohorts) were analyzed, consisting 347 (20.59%) in high pretreatment NLR value group and 1338 (79.41%) in low pretreatment NLR value one. The results demonstrated that elevated pretreatment NLR was significantly related to poor OS (HR 2.17, 95% CI 1.82–2.58) and RFS (HR 1.96, 95% CI 1.64–2.35) in patients with CRLM. Conclusion The result of this systematic review and meta-analysis indicated that an elevated pretreatment NLR was closely correlated with poor long-term survival (OS and RFS) in CRLM patients. NLR can be routinely monitored and serve as a useful and cost-effective marker with strong prognostic significance in patients with CRLM.

Beschreibung: by Peter C. Rowe, Kevin R. Fontaine, Megan Lauver, Samantha E. Jasion, Colleen L. Marden, Malini Moni, Carol B. Thompson, Richard L. Violand Chronic fatigue syndrome (CFS) is a complex, multisystem disorder that can be disabling. CFS symptoms can be provoked by increased physical or cognitive activity, and by orthostatic stress. In preliminary work, we noted that CFS symptoms also could be provoked by application of longitudinal neural and soft tissue strain to the limbs and spine of affected individuals. In this study we measured the responses to a straight leg raise neuromuscular strain maneuver in individuals with CFS and healthy controls. We randomly assigned 60 individuals with CFS and 20 healthy controls to either a 15 minute period of passive supine straight leg raise (true neuromuscular strain) or a sham straight leg raise. The primary outcome measure was the symptom intensity difference between the scores during and 24 hours after the study maneuver compared to baseline. Fatigue, body pain, lightheadedness, concentration difficulties, and headache scores were measured individually on a 0–10 scale, and summed to create a composite symptom score. Compared to individuals with CFS in the sham strain group, those with CFS in the true strain group reported significantly increased body pain (P = 0.04) and concentration difficulties (P = 0.02) as well as increased composite symptom scores (all P = 0.03) during the maneuver. After 24 hours, the symptom intensity differences were significantly greater for the CFS true strain group for the individual symptom of lightheadedness (P = 0.001) and for the composite symptom score (P = 0.005). During and 24 hours after the exposure to the true strain maneuver, those with CFS had significantly higher individual and composite symptom intensity changes compared to the healthy controls. We conclude that a longitudinal strain applied to the nerves and soft tissues of the lower limb is capable of increasing symptom intensity in individuals with CFS for up to 24 hours. These findings support our preliminary observations that increased mechanical sensitivity may be a contributor to the provocation of symptoms in this disorder.

Beschreibung: by Shweta Mendiratta, Shipra Bhatia, Shruti Jain, Taniya Kaur, Vani Brahmachari The presence of a highly conserved DNA binding domain in INO80 subfamily predicted that INO80 directly interacts with DNA and we demonstrated its DNA binding activity in vitro . Here we report the consensus motif recognized by the DBINO domain identified by SELEX method and demonstrate the specific interaction of INO80 with the consensus motif. We show that INO80 significantly down regulates the reporter gene expression through its binding motif, and the repression is dependent on the presence of INO80 but not YY1 in the cell. The interaction is lost if specific residues within the consensus motif are altered. We identify a large number of potential target sites of INO80 in the human genome through in silico analysis that can grouped into three classes; sites that contain the recognition sequence for INO80 and YY1, only YY1 and only INO80. We demonstrate the binding of INO80 to a representative set of sites in HEK cells and the correlated repressive histone modifications around the binding motif. In the light of the role of INO80 in homeotic gene regulation in Drosophila as an Enhancer of trithorax and polycomb protein (ETP) that can modify the effect of both repressive complexes like polycomb as well as the activating complex like trithorax, it remains to be seen if INO80 can act as a recruiter of chromatin modifying complexes.

Beschreibung: Publication date: Available online 18 July 2016 Source: Journal of Asian Ceramic Societies Author(s): J. Raj Mohamed, L. Amalraj The present work investigates the effect of precursor concentration ( m c ) on the structural, optical, morphological and electrical conductivity properties of In 2 S 3 thin films grown on amorphous glass substrates by nebulized spray pyrolysis (NSP) technique. The mixed phase of cubic and tetragonal structure of In 2 S 3 thin films at higher concentration has been observed by X-ray diffraction pattern. The reduced strain by increasing the precursor concentration increased the average crystallite from 17.8 to 28.9 nm. The energy dispersive analysis by X-ray (EDAX) studies confirmed the presence of In and S. The transmittance, optical direct band gap energy, Urbach energy and skin depth of In 2 S 3 films have been analyzed by optical absorption spectra. The better conductivity and mobility noticed at m c = 0.15 M are explained by carrier concentration and crystallite. Better optical and electrical conductivity behaviour of In 2 S 3 thin film sample proposes for effective solar cell fabrication.

Beschreibung: Global combustion sources of organic aerosols: model comparison with 84 AMS factor-analysis data sets Alexandra P. Tsimpidi, Vlassis A. Karydis, Spyros N. Pandis, and Jos Lelieveld Atmos. Chem. Phys., 16, 8939-8962, doi:10.5194/acp-16-8939-2016, 2016 In this work we use a global chemistry climate model together with a comprehensive global AMS data set in order to provide valuable insights into the temporal and geographical variability of the contribution of the emitted particles and the chemically processed organic material from combustion sources to total OA. This study reveals the high importance of SOA from anthropogenic sources on global OA concentrations and identifies plausible sources of discrepancy between models and measurements.

Beschreibung: Modeling investigation of light absorbing aerosols in the central Amazon during the wet season Qiaoqiao Wang, Jorge Saturno, Xuguang Chi, David Walter, Jost V. Lavric, Daniel Moran- Zuloaga, Florian Ditas, Christopher Pöhlker, Joel Brito, Samara Carbone, Paulo Artaxo, and Meinrat O. Andreae Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-586,2016 Manuscript under review for ACP (discussion: open, 0 comments) We use a chemical transport model to interpret observed aerosol concentrations and absorption over the Amazon basin during the wet season. With daily temporal resolution for open fire emissions and modified aerosol optical properties, our model successfully captures the observed variation of aerosol concentrations and absorption over the Amazon basin. The simulation indicates important influence of open fire mainly from northern South America and from Northern Africa in the wet season.

Beschreibung: Morphology and Mixing of BC Particles Collected in Central California During the CARES Field Study Ryan C. Moffet, Rachel E. O'Brien, Peter Alpert, Stephen T. Kelly, Don Q. Pham, Mary K. Gilles, Daniel Knopf, and Alexander Laskin Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-634,2016 Manuscript under review for ACP (discussion: open, 0 comments) Atmospheric black carbon (BC), commonly known as soot, is an important constituent of the earth's that imparts a warming similar to that of carbon dioxide. However, BC is much shorter lived and has uncertain warming due to its mixture with other solid and liquid components. Here, advanced microscopic methods have provided a detailed look at thousands of BC particles sampled from central California; these measurements will lead towards a better understanding of the effects that BC has on climate.

Beschreibung: 4-D-VAR assimilation of disdrometer data and radar spectral reflectivities for raindrop size distribution and vertical wind retrievals François Mercier, Aymeric Chazottes, Laurent Barthès, and Cécile Mallet Atmos. Meas. Tech., 9, 3145-3163, doi:10.5194/amt-9-3145-2016, 2016 The aim of this study is to retrieve vertical profiles of raindrop size distributions and vertical winds from radar and ground measurements. This is crucial to understand the phenomena acting on the raindrops at small scale during their fall and then to be able to merge measurements of rain at different heights and scales (from radar, rain gauges, satellites etc.). It could also help to improve the treatment of radar data and to better parameterize rain in numerical weather prediction models.

Beschreibung: Diversity and mineral substrate preference in endolithic microbial communities from marine intertidal outcrops (Isla de Mona, Puerto Rico) Estelle Couradeau, Daniel Roush, Brandon Scott Guida, and Ferran Garcia-Pichel Biogeosciences Discuss., doi:10.5194/bg-2016-254,2016 Manuscript under review for BG (discussion: open, 0 comments) Endolithic (inside rock) microbial communities are dominated by cyanobacteria, among which the true boring cyanobacteria actively perforate the mineral and play a significant role in the erosion of coastal outcrops that may increase with ocean acidification. We interrogated the microbial communities associated with various intertidal substrates of Mona Island (PR) and brought the first evidence that there exists a mineralogical substrate preference among the pioneers true boring cyanobacteria.

Beschreibung: Influence of the spatial distribution of gravity wave activity on the middle atmospheric circulation and transport Petr Šácha, Friederike Lilienthal, Christoph Jacobi, and Petr Pišoft Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-548,2016 Manuscript under review for ACP (discussion: open, 0 comments) With a mechanistic model for the middle and upper atmosphere we performed sensitivity simulations to study a possible effect of a localized GW breaking hotspot in the Eastern Asia/Northern Pacific region and also more generally, possible influence of spatial distribution of gravity wave activity on the middle atmospheric circulation and transport. We show implications for polar vortex stability, in-situ planetary wave generation and longitudinal variability and strength of the B.–D. circulation.

Beschreibung: Influence of enhanced Asian NO x emissions on ozone in the Upper Troposphere and Lower Stratosphere (UTLS) in chemistry climate model simulations Chaitri Roy, Suvarna Fadnavis, Rolf Müller, Ayantika Dey Chaudhary, and Felix Ploeger Atmos. Chem. Phys. Discuss., doi:10.5194/acp-2016-582,2016 Manuscript under review for ACP (discussion: open, 0 comments) In the monsoon season, Asian NO x emissions are rapidly transported to the UTLS and can impact ozone in the UTLS. From chemistry climate model simulations, we show that increasing Asian NO x emissions have led to enhanced ozone radiative forcing over South east Asia which leads to significant warming over the Tibetan plateau and increase precipitations over India. However, a further increase in NO x emissions elicited negative precipitation via ozone heating over the Tibetan plateau.

Beschreibung: A reduced-complexity model for sediment transport and step-pool morphology Matteo Saletti, Peter Molnar, Marwan A. Hassan, and Paolo Burlando Earth Surf. Dynam., 4, 549-566, doi:10.5194/esurf-4-549-2016, 2016 This study presents a new reduced-complexity model with few parameters linked to basic physical processes, which aims to reproduce the transport of sediment as bed load and the formation and stability of channel morphology in steep mountain streams. The model is able to simulate the formation and stability of steps, bed structures commonly encountered in steep channels, by assuming that their formation is due to intense sediment transport during high flows causing jamming of particles.

Beschreibung: Publication date: November 2016 Source: Energy Reports, Volume 2 Author(s): Sangram Bana, R.P. Saini In order to predict the performance of a PV system, a reliable and accurate simulation design of PV systems before being installed is a necessity. The present study concerns the development of single and double diode model of solar PV system and ensures the best suited model under specific environmental condition for accurate performance prediction. The information provided in the manufacturers’ data sheet is not sufficient for developing a Simulink based single and double diode models of PV module. These parameters are crucial to predict accurate performance of a PV module. These parameters of the proposed solar PV models have been calculated using an efficient iterative technique. This paper compares the simulation results of both the models with manufacturer’s data sheet to investigate the accuracy and validity. A MATLAB/Simulink based comparative performance analysis of these models under inconsistent atmospheric conditions and the effect of variations in model parameters has been carried out. Despite the simplicity, these models are highly sensitive and respond to a slight variation in temperature and insolation. It is observed that double diode PV model is more accurate under low intensity insolation or shading condition. The performance evaluation of the models under present study will be helpful to understand the I-V curves, which will enable us in predicting the solar PV system power production under variable input conditions.

Beschreibung: A new test statistic for climate models that includes field and spatial dependencies using Gaussian Markov random fields Alvaro Nosedal-Sanchez, Charles S. Jackson, and Gabriel Huerta Geosci. Model Dev., 9, 2407-2414, doi:10.5194/gmd-9-2407-2016, 2016 We have developed a new metric for climate model evaluation that quantifies the significance of modeling errors across multiple fields. Our approach dramatically reduces the amount of data that is required to evaluate field and space dependencies and increases the community's potential to make use of the extremely valuable but limited satellite observational record. Our objective is to improve the strategies that currently exist for more formal data-driven model development.

Beschreibung: Publication date: July 2016 Source: Global Ecology and Conservation, Volume 7 Author(s): Lina Mtwana Nordlund How do you, as a university lecturer, change from teacher-centered teaching to a more student-centered, active teaching? This paper aims to inspire you to make a change, big or small, to increase your students’ engagement and learning, by presenting suggestions on what you can do. The ideas and suggestions synthesized here are based on several different teaching philosophies and methods, which are well tested and shown to be effective in the right setting. The selection of suggestions is believed to be specifically suitable for ecology. The paper includes suggestions on how to plan a course or a lecture by setting a good learning environment. Both pre-lecture activities and during lecture activities are included, with a focus on activities to engage students and encourage increased discussion and reflections, as well as what to think about when choosing learning activities and how and why it is important to teach students to think and act like professionals in ecology. While changing teaching methods takes investment of time, time that is limited for many researchers, even small changes in your teaching can make big differences in learning, and the investment will hopefully pay back by making teaching more fun and rewarding. The suggestions presented are understandable without being be conversant in the ‘education literature’, but will provide you with a vocabulary of teaching activities that will be useful if you are inspired to find more information and learn more about teaching.

Beschreibung: Looking beyond general metrics for model comparison – lessons from an international model intercomparison study Tanja de Boer-Euser, Laurène Bouaziz, Jan De Niel, Claudia Brauer, Benjamin Dewals, Gilles Drogue, Fabrizio Fenicia, Benjamin Grelier, Jiri Nossent, Fernando Pereira, Hubert Savenije, Guillaume Thirel, and Patrick Willems Hydrol. Earth Syst. Sci. Discuss., doi:10.5194/hess-2016-339,2016 Manuscript under review for HESS (discussion: open, 0 comments) In this study the rainfall–runoff models of eight international research groups were compared for a set of subcatchments of the Meuse basin to investigate the influence of certain model components on the modelled discharge. Although the models showed similar performances based on general metrics, clear differences could be observed for specific events. The differences during drier conditions could indeed be linked back to differences in model structures.

Beschreibung: by Michal Cagalinec, Mailis Liiv, Zuzana Hodurova, Miriam Ann Hickey, Annika Vaarmann, Merle Mandel, Akbar Zeb, Vinay Choubey, Malle Kuum, Dzhamilja Safiulina, Eero Vasar, Vladimir Veksler, Allen Kaasik Deficiency of the protein Wolfram syndrome 1 (WFS1) is associated with multiple neurological and psychiatric abnormalities similar to those observed in pathologies showing alterations in mitochondrial dynamics. The aim of this study was to examine the hypothesis that WFS1 deficiency affects neuronal function via mitochondrial abnormalities. We show that down-regulation of WFS1 in neurons leads to dramatic changes in mitochondrial dynamics (inhibited mitochondrial fusion, altered mitochondrial trafficking, and augmented mitophagy), delaying neuronal development. WFS1 deficiency induces endoplasmic reticulum (ER) stress, leading to inositol 1,4,5-trisphosphate receptor (IP 3 R) dysfunction and disturbed cytosolic Ca 2+ homeostasis, which, in turn, alters mitochondrial dynamics. Importantly, ER stress, impaired Ca 2+ homeostasis, altered mitochondrial dynamics, and delayed neuronal development are causatively related events because interventions at all these levels improved the downstream processes. Our data shed light on the mechanisms of neuronal abnormalities in Wolfram syndrome and point out potential therapeutic targets. This work may have broader implications for understanding the role of mitochondrial dynamics in neuropsychiatric diseases.

Beschreibung: by Nandhitha Subramanian, Amanda J. Scopelliti, Jane E. Carland, Renae M. Ryan, Megan L. O’Mara, Robert J. Vandenberg

Beschreibung: by Byung-Whi Kong, Kentu Lassiter, Alissa Piekarski-Welsher, Sami Dridi, Antonio Reverter, Nicholas James Hudson, Walter Gay Bottje

Beschreibung: by Steve Jordan, J. Joseph Giersch, Clint C. Muhlfeld, Scott Hotaling, Liz Fanning, Tyler H. Tappenbeck, Gordon Luikart

Beschreibung: by Kaylyn L. Devlin, Tiffany Sanford, Lauren M. Harrison, Paul LeBourgeois, Laura M. Lashinger, Elizabeth Mambo, Stephen D. Hursting MicroRNAs have emerged as ubiquitous post-transcriptional regulators that coordinate many fundamental processes within cells, including those commonly linked to cancer when dysregulated. Profiling microRNAs across stages of cancer progression provides focus as to which microRNAs are key players in cancer development and are therefore important to manipulate with interventions to delay cancer onset and progression. Calorie restriction is one of the most effective preventive interventions across many types of cancer, although its effects on microRNAs have not been well characterized. We used the dimethylbenz[a]-anthracene-induced model of luminal mammary cancer in Sprague Dawley rats to elucidate which microRNAs are linked to progression in this type of cancer and, subsequently, to study how calorie restriction affects such microRNAs. We identified eight microRNAs (miR-10a, miR-10b, miR-21, miR-124, miR-125b, miR-126, miR-145 and miR-200a) to be associated with DMBA-induced mammary tumor progression. Calorie restriction, which greatly increased tumor-free survival and decreased the overall size of tumors that did develop, significantly decreased the expression of one microRNA, miR-200a, which was positively associated with tumor progression. We further showed that inhibition of miR-200a function, mimicking the effect of calorie restriction on this microRNA, inhibited proliferation in both rat (LA7) and human (MCF7) luminal mammary cancer cell lines. These findings present, for the first time, a stage-specific profile of microRNAs in a rodent model of luminal mammary cancer. Furthermore, we have identified the regulation of miR-200a, a microRNA that is positively associated with progression in this model, as a possible mechanism contributing to the anticancer effects of calorie restriction.

Beschreibung: by Fiorenzo Moscatelli, Giovanni Messina, Anna Valenzano, Vincenzo Monda, Andrea Viggiano, Antonietta Messina, Annamaria Petito, Antonio Ivano Triggiani, Michela Anna Pia Ciliberti, Marcellino Monda, Laura Capranica, Giuseppe Cibelli

Beschreibung: by Marcos Iglesias, Juan Jesús Augustin, Pilar Alvarez, Inés Santiuste, Jorge Postigo, Jesús Merino, Ramón Merino The inhibition of apoptotic cell death in T cells through the dysregulated expression of BCL2 family members has been associated with the protection against the development of different autoimmune diseases. However, multiple mechanisms were proposed to be responsible for such protective effect. The purpose of this study was to explore the effect of the T-cell overexpression of BCL2A1, an anti-apoptotic BCL2 family member without an effect on cell cycle progression, in the development of collagen-induced arthritis. Our results demonstrated an attenuated development of arthritis in these transgenic mice. The protective effect was unrelated to the suppressive activity of regulatory T cells but it was associated with a defective activation of p38 mitogen-activated protein kinase in CD4 + cells after in vitro TCR stimulation. In addition, the in vitro and in vivo T H 17 differentiation were impaired in BCL2A1 transgenic mice. Taken together, we demonstrated here a previously unknown role for BCL2A1 controlling the activation of CD4 + cells and their differentiation into pathogenic proinflammatory T H 17 cells and identified BCL2A1 as a potential target in the control of autoimmune/inflammatory diseases.

Beschreibung: by Tianle Chao, Guizhi Wang, Jianmin Wang, Zhaohua Liu, Zhibin Ji, Lei Hou, Chunlan Zhang High-throughput mRNA sequencing enables the discovery of new transcripts and additional parts of incompletely annotated transcripts. Compared with the human and cow genomes, the reference annotation level of the sheep genome is still low. An investigation of new transcripts in sheep skeletal muscle will improve our understanding of muscle development. Therefore, applying high-throughput sequencing, two cDNA libraries from the biceps brachii of small-tailed Han sheep and Dorper sheep were constructed, and whole-transcriptome analysis was performed to determine the unknown transcript catalogue of this tissue. In this study, 40,129 transcripts were finally mapped to the sheep genome. Among them, 3,467 transcripts were determined to be unannotated in the current reference sheep genome and were defined as new transcripts. Based on protein-coding capacity prediction and comparative analysis of sequence similarity, 246 transcripts were classified as portions of unannotated genes or incompletely annotated genes. Another 1,520 transcripts were predicted with high confidence to be long non-coding RNAs. Our analysis also revealed 334 new transcripts that displayed specific expression in ruminants and uncovered a number of new transcripts without intergenus homology but with specific expression in sheep skeletal muscle. The results confirmed a complex transcript pattern of coding and non-coding RNA in sheep skeletal muscle. This study provided important information concerning the sheep genome and transcriptome annotation, which could provide a basis for further study.

Beschreibung: by Simina M. Boca, Maki Nishida, Michael Harris, Shruti Rao, Amrita K. Cheema, Kirandeep Gill, Difei Wang, Lin An, Robinder Gauba, Haeri Seol, Lauren P. Morgenroth, Erik Henricson, Craig McDonald, Jean K. Mah, Paula R. Clemens, Eric P. Hoffman, Yetrib Hathout, Subha Madhavan

Beschreibung: by Xiaolin Qin, Heping Zheng, Yaohua Xue, Xuqi Ren, Bin Yang, Jinmei Huang, Shujie Huang, Xingzhong Wu, Weiying Zeng, Jiangli Ou, Yinyuan Lan, Sanmei Tang Background Chlamydia trachomatis is one of the most prevalent bacterial sexually transmitted infection in China. Although C . trachomatis genotypes can be discriminated by outer membrane protein gene ( omp A) sequencing, currently available methods have limited resolutions. This study used a high-resolution genotyping method, namely, multilocus variable number tandem-repeat analysis with omp A sequencing (MLVA)- omp A, to investigate the local epidemiology of C . trachomatis infections among men who have sex with men (MSM) and men who have sex with women (MSW) attending a sexually transmitted diseases (STD) clinic in Guangzhou, China. Methods Rectal specimens from MSM and urethral specimens from MSW were collected between January 2013 and July 2014 at the Guangdong Provincial Center STD clinic. The specimens were sent to the laboratory for analyses. All specimens that were tested positive for C . trachomatis by the commercial nucleic acid amplification tests were genotyped by MLVA- omp A. Results Fifty-one rectal specimens from MSM and 96 urethral specimens from MSW were identified with C . trachomatis . One hundred and forty-four of the 147 specimens were fully genotyped by MLVA- omp A. Rectal specimens from MSM were divided into four omp A genotypes and urethral specimens from MSW into nine genotypes. No mixed infections were found among all specimens. The most frequent genotypes were D, G, J, E and F. All specimens were further divided into 46 types after omp A genotyping was combined with MLVA. Genotypes D-8.7.1 and G-3.4a.3 were the most frequent among MSM, whereas genotypes D-3.4a.4, E-8.5.1, F-8.5.1, and J-3.4a.2 were the most frequent subtypes among MSW. The discriminatory index D was 0.90 for MLVA, 0.85 for omp A, and 0.95 for MLVA- omp A. Conclusions The most prevalent MLVA- omp A genotypes were significantly different between MSM and MSW from Guangzhou, China. Moreover, MLVA- omp A represented a more favorable degree of discrimination than omp A and could be a reliable complement for omp A for the routine subtypes of C . trachomatis .

Beschreibung: by Siiri Latvala, Jonas Hedberg, Sebastiano Di Bucchianico, Lennart Möller, Inger Odnevall Wallinder, Karine Elihn, Hanna L. Karlsson Occupational exposure to airborne nickel is associated with an elevated risk for respiratory tract diseases including lung cancer. Therefore, the increased production of Ni-containing nanoparticles necessitates a thorough assessment of their physical, chemical, as well as toxicological properties. The aim of this study was to investigate and compare the characteristics of nickel metal (Ni) and nickel oxide (NiO) particles with a focus on Ni release, reactive oxygen species (ROS) generation, cellular uptake, cytotoxicity and genotoxicity. Four Ni-containing particles of both nano-size (Ni-n and NiO-n) and micron-size (Ni-m1 and Ni-m2) were tested. The released amount of Ni in solution was notably higher in artificial lysosomal fluid (e.g. 80–100 wt% for metallic Ni) than in cell medium after 24h (ca. 1–3 wt% for all particles). Each of the particles was taken up by the cells within 4 h and they remained in the cells to a high extent after 24 h post-incubation. Thus, the high dissolution in ALF appeared not to reflect the particle dissolution in the cells. Ni-m1 showed the most pronounced effect on cell viability after 48 h (alamar blue assay) whereas all particles showed increased cytotoxicity in the highest doses (20–40 μg cm 2 ) when assessed by colony forming efficiency (CFE). Interestingly an increased CFE, suggesting higher proliferation, was observed for all particles in low doses (0.1 or 1 μg cm -2 ). Ni-m1 and NiO-n were the most potent in causing acellular ROS and DNA damage. However, no intracellular ROS was detected for any of the particles. Taken together, micron-sized Ni (Ni-m1) was more reactive and toxic compared to the nano-sized Ni. Furthermore, this study underlines that the low dose effect in terms of increased proliferation observed for all particles should be further investigated in future studies.

Beschreibung: by The PLOS ONE Staff

Beschreibung: by Sandra J. Connelly, James A. Stoeckel, Robert A. Gitzen, Craig E. Williamson, Maria J. González Recent studies have demonstrated substantial effects of environmental stress that vary among clones. Exposure to ultraviolet radiation (UV) is an important abiotic stressor that is highly variable in aquatic ecosystems due to diel and seasonal variations in incident sunlight as well as to differences in the UV transparency of water among water bodies, the depth distribution of organisms, and the ability of organisms to detect and respond to UV. In contrast to the convention that all UV is damaging, evidence is accumulating for the beneficial effects of exposure to low levels of UV radiation. Whereas UV has been frequently observed as the primary light-related stressor, herein we present evidence that dark conditions may be similarly “stressful” (reduction of overall fitness), and stress responses vary among clones of the freshwater crustacean Daphnia parvula . We have identified a significant relationship between survivorship and reduced fecundity of clones maintained in dark conditions, but no correlation between tolerance of the clones to dark and UV radiation. Low tolerance to dark conditions can have negative effects not only on accumulated stresses in organisms (e.g. the repair of UV-induced damage in organisms with photolyase), but potentially on the overall physiology and fitness of organisms. Our results support recent evidence of the beneficial effects of low-level UV exposure for some organisms.

Beschreibung: by Bonnie K. Harrington, Heather L. Gardner, Raquel Izumi, Ahmed Hamdy, Wayne Rothbaum, Kevin R. Coombes, Todd Covey, Allard Kaptein, Michael Gulrajani, Bart Van Lith, Cecile Krejsa, Christopher C. Coss, Duncan S. Russell, Xiaoli Zhang, Bridget K. Urie, Cheryl A. London, John C. Byrd, Amy J. Johnson, William C. Kisseberth Acalabrutinib (ACP-196) is a second-generation inhibitor of Bruton agammaglobulinemia tyrosine kinase (BTK) with increased target selectivity and potency compared to ibrutinib. In this study, we evaluated acalabrutinib in spontaneously occurring canine lymphoma, a model of B-cell malignancy similar to human diffuse large B-cell lymphoma (DLBCL). First, we demonstrated that acalabrutinib potently inhibited BTK activity and downstream effectors in CLBL1, a canine B-cell lymphoma cell line, and primary canine lymphoma cells. Acalabrutinib also inhibited proliferation in CLBL1 cells. Twenty dogs were enrolled in the clinical trial and treated with acalabrutinib at dosages of 2.5 to 20mg/kg every 12 or 24 hours. Acalabrutinib was generally well tolerated, with adverse events consisting primarily of grade 1 or 2 anorexia, weight loss, vomiting, diarrhea and lethargy. Overall response rate (ORR) was 25% (5/20) with a median progression free survival (PFS) of 22.5 days. Clinical benefit was observed in 30% (6/20) of dogs. These findings suggest that acalabrutinib is safe and exhibits activity in canine B-cell lymphoma patients and support the use of canine lymphoma as a relevant model for human non-Hodgkin lymphoma (NHL).

Beschreibung: by Mari Tohya, Takayasu Watanabe, Fumito Maruyama, Sakura Arai, Atsushi Ota, Taryn B. T. Athey, Nahuel Fittipaldi, Ichiro Nakagawa, Tsutomu Sekizaki Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S . suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs), ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels) ranging from one to 10 4 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated) bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes.

Beschreibung: by Yuki Nakano, Takeru Shimazaki, Nobuko Kobayashi, Yukiko Miyoshi, Aoi Ono, Mamoru Kobayashi, Chieko Shiragami, Kazuyuki Hirooka, Akitaka Tsujikawa Purpose To establish the normative database of retinal oximetry using Oxymap T1 in a healthy Japanese population, and study the reproducibility of the measurements in Japanese. Methods We measured oxygen saturation in the major retinal vessels with Oxymap T1 in 252 eyes of 252 healthy Japanese subjects. Fundus images acquired using Oxymap T1 were processed using built-in Oxymap Analyzer software. Reproducibility of retinal oximetry was investigated using 20 eyes of 20 healthy subjects. Results The mean retinal oxygen saturation of 4 quadrants in healthy Japanese was 97.0 ± 6.9% in arteries and 52.8 ± 8.3% in veins. The mean arteriovenous difference in oxygen saturation was 44.2 ± 9.2%. Both arterial and venous oxygen saturation were significantly lower in the temporal side of the retina, especially in the temporal-inferior vessels. However, the arteriovenous difference in oxygen saturation was limited in the 4 quadrants. Interphotograph, intervisit, and interevaluator intraclass correlation coefficients were 0.936–0.979, 0.809–0.837, and 0.732–0.947, respectively. In the major retinal arteries, oxygen saturation increased with age (r = 0.18, p

Beschreibung: by Guang Yang, Hongqin Xu, Huiping Zhang, Qiong Yu, Yanhua Wu, Jieping Shi, Wenwang Rao, Yueyue You, Changgui Kou, Yaqin Yu Objective The purpose of this study was to explore the association between single nucleotide polymorphisms (SNPs) in the phospholipase A2 (PLA2), group XIIA gene ( PLA2G12A ) and schizophrenia. Methods This study included 1,063 schizophrenia patients and 1,103 healthy controls from a Han Chinese Population in Northeast China. Four tagSNPs (rs11728699 in intron 1, synonymous rs2285714 in exon 3, rs3087494 in the 3’ UTR, and rs7694620 in the downstream region) in PLA2G12A were selected, and they were genotyped by the MALDI-TOF-MS technology. The Chi-square (χ 2 ) test and haplotype analysis were performed to analyze the association of PLA2G12A SNPs and schizophrenia using the software packages SPSS 16.0 and Haploview 4.2. Results Among the four tagSNPs, only SNP rs3087494 in the 3’ UTR of PLA2G12A showed significant differences in both allele frequencies (χ 2 = 20.136, P

Beschreibung: by Robert A. Colvin, Qiaoling Jin, Barry Lai, Lech Kiedrowski Increasing evidence suggests that metal dyshomeostasis plays an important role in human neurodegenerative diseases. Although distinctive metal distributions are described for mature hippocampus and cortex, much less is known about metal levels and intracellular distribution in individual hippocampal neuronal somata. To solve this problem, we conducted quantitative metal analyses utilizing synchrotron radiation X-Ray fluorescence on frozen hydrated primary cultured neurons derived from rat embryonic cortex (CTX) and two regions of the hippocampus: dentate gyrus (DG) and CA1. Comparing average metal contents showed that the most abundant metals were calcium, iron, and zinc, whereas metals such as copper and manganese were less than 10% of zinc. Average metal contents were generally similar when compared across neurons cultured from CTX, DG, and CA1, except for manganese that was larger in CA1. However, each metal showed a characteristic spatial distribution in individual neuronal somata. Zinc was uniformly distributed throughout the cytosol, with no evidence for the existence of previously identified zinc-enriched organelles, zincosomes. Calcium showed a peri-nuclear distribution consistent with accumulation in endoplasmic reticulum and/or mitochondria. Iron showed 2–3 distinct highly concentrated puncta only in peri-nuclear locations. Notwithstanding the small sample size, these analyses demonstrate that primary cultured neurons show characteristic metal signatures. The iron puncta probably represent iron-accumulating organelles, siderosomes. Thus, the metal distributions observed in mature brain structures are likely the result of both intrinsic neuronal factors that control cellular metal content and extrinsic factors related to the synaptic organization, function, and contacts formed and maintained in each region.

Beschreibung: by Yasuhiro Takahashi, Yoshiyuki Kitaguchi, Shunsuke Nakakura, Hidenori Mito, Akiko Kimura, Hirohiko Kakizaki Purpose To examine the characteristics of excyclotropia correction through surgery on the inferior rectus muscle in patients with thyroid eye disease. Methods This was a retrospective, observational study at a single institution. We reviewed 36 patients who had undergone unilateral inferior rectus muscle recession, with or without nasal inferior rectus muscle transposition. The following factors were investigated as possibly influencing excyclotropia correction: inferior rectus muscle thickness, degree of adipose change in the inferior rectus muscle, smoking status, history of orbital radiotherapy, and the amount of inferior rectus muscle recession. Using T1-weighted coronal magnetic resonance imaging, we measured the cross-sectional area of the inferior rectus muscle at its largest point, as well as the bright-signal area of the inferior rectus muscle, which reflects intermuscular adipose change. We then calculated the percentage internal bright-signal area at the point of the largest inferior rectus muscle cross-sectional area. The history of orbital radiotherapy was graded using a binary system. We evaluated correlations among excyclotropia correction, the amount of nasal inferior rectus muscle transposition, and the possible influencing factors listed, using stepwise multiple regression analyses. Results The multiple regression model demonstrated a significant relationship among excyclotropia correction, amount of nasal inferior rectus muscle transposition, and the amount of inferior rectus muscle recession (Y CORRECTION = 8.546X TENDON WIDTH + 0.405X RECESSION − 0.908; r = 0.844; adjusted r 2 = 0.695; P 〈 0.001). Conclusions Excyclotropia correction was correlated with the amount of nasal inferior rectus muscle transposition and the amount of inferior rectus muscle recession, but not with the other factors. The regression model presented in this study will enable us to determine more precisely the amount of nasal inferior rectus muscle transposition in patients with excyclotropia of various angles.

Beschreibung: by Qi Zhao, Yun Zhu, Lyle G. Best, Jason G. Umans, Karan Uppal, ViLinh T. Tran, Dean P. Jones, Elisa T. Lee, Barbara V. Howard, Jinying Zhao Obesity is a typical metabolic disorder resulting from the imbalance between energy intake and expenditure. American Indians suffer disproportionately high rates of obesity and diabetes. The goal of this study is to identify metabolic profiles of obesity in 431 normoglycemic American Indians participating in the Strong Heart Family Study. Using an untargeted liquid chromatography–mass spectrometry, we detected 1,364 distinct m/z features matched to known compounds in the current metabolomics databases. We conducted multivariate analysis to identify metabolic profiles for obesity, adjusting for standard obesity indicators. After adjusting for covariates and multiple testing, five metabolites were associated with body mass index and seven were associated with waist circumference. Of them, three were associated with both. Majority of the obesity-related metabolites belongs to lipids, e.g., fatty amides, sphingolipids, prenol lipids, and steroid derivatives. Other identified metabolites are amino acids or peptides. Of the nine identified metabolites, five metabolites (oleoylethanolamide, mannosyl-diinositol-phosphorylceramide, pristanic acid, glutamate, and kynurenine) have been previously implicated in obesity or its related pathways. Future studies are warranted to replicate these findings in larger populations or other ethnic groups.

Beschreibung: by Zuzana Zákostelská, Jana Málková, Klára Klimešová, Pavel Rossmann, Michaela Hornová, Iva Novosádová, Zuzana Stehlíková, Martin Kostovčík, Tomáš Hudcovic, Renata Štepánková, Kateřina Jůzlová, Jana Hercogová, Helena Tlaskalová-Hogenová, Miloslav Kverka Psoriasis is a chronic inflammatory skin disease in which Th17 cells play a crucial role. Since indigenous gut microbiota influences the development and reactivity of immune cells, we analyzed the link among microbiota, T cells and the formation of psoriatic lesions in the imiquimod-induced murine model of psoriasis. To explore the role of microbiota, we induced skin inflammation in germ-free (GF), broad-spectrum antibiotic (ATB)-treated or conventional (CV) BALB/c and C57BL/6 mice. We found that both mice reared in GF conditions for several generations and CV mice treated with ATB were more resistant to imiquimod-induced skin inflammation than CV mice. The ATB treatment dramatically changed the diversity of gut bacteria, which remained stable after subsequent imiquimod application; ATB treatment resulted in a substantial increase in the order Lactobacillales and a significant decrease in Coriobacteriales and Clostridiales . Moreover, as compared to CV mice, imiquimod induced a lower degree of local and systemic Th17 activation in both GF and ATB-treated mice. These findings suggest that gut microbiota control imiquimod-induced skin inflammation by altering the T cell response.

Beschreibung: by Lu Zhang, Wen Wang, Yan Gao, Jie Lan, Lixin Xie Purpose To compare the efficacy and safety of current treatments in diabetic macular edema (DME). Methods PubMed, Embase and CENTRAL were systematically reviewed for randomized controlled trials of current treatments in DME through August 2015. Data on the mean change of best-corrected visual acuity (BCVA) and central macular thickness (CMT) were extracted, and adverse events (AEs) were collected. Results A total of 21 trials were included in our network meta-analysis. Intravitreal ranibizumab improved BCVA most significantly (OR: +7.01 95%CI (2.56 to 11.39)) in 6 months and intravitreal aflibercept (+8.19 (5.07 to 11.96)) in 12 months. Intravitreal triamcinolone combined with LASER decreased CMT most significantly (-111.34 (-254.61 to 37.93)) in 6 months and intravitreal aflibercept (-110.83 (-190.25 to -35.27)) in 12 months. Compared with the relatively high rate of ocular AEs in the groups with administration of steroids, systematic AEs occurred more frequently in the groups with vascular endothelial growth factor inhibitors involved. Conclusions Our analysis confirms that intravitreal aflibercept is most favorable with both BCVA improvement and CMT decrease than other current therapies in the management of DME within 12 months. Vascular endothelial growth factor inhibitors for DME should be used with caution due to systematic AEs. Combined intravitreal triamcinolone with LASER has a stronger efficacy in decreasing CMT than the other interventions in the early stage after injection. More high-quality randomized controlled trials will be necessary.

Beschreibung: by Stefania Balzarotti, Barbara Colombo The dorsolateral prefrontal cortex (DLPFC) is generally thought to be involved in affect and emotional processing; however, the specific contribution of each hemisphere continues to be debated. In the present study, we employed unilateral tDCS to test the unique contribution of left DLPFC in the encoding and retrieval of emotional stimuli in healthy subjects. Forty-two right handed undergraduate students received either anodal, cathodal or sham stimulation of left DLPFC while viewing neutral, pleasant, and unpleasant pictures. After completing a filler task, participants were asked to remember as many pictures as possible. Results showed that participants were able to remember a larger amount of emotional (both pleasant and unpleasant) pictures than of neutral ones, regardless of the type of tDCS condition. Participants who received anodal stimulation recalled a significantly higher number of pleasant images than participants in the sham and cathodal conditions, while no differences emerged in the recall of neutral and unpleasant pictures. We conclude that our results provide some support to the role of left prefrontal cortex in the encoding and retrieval of pleasant stimuli.

Beschreibung: by Daiana Priscila Rodrigues-de-Souza, Domingo Palacios-Ceña, Lourdes Moro-Gutiérrez, Paula Rezende Camargo, Tania Fátima Salvini, Francisco Alburquerque-Sendín Background Low back pain (LBP) could be influenced by socio-cultural factors. Pain narratives are important to understand the influence of environment on patients with chronic LBP. There are few studies that have explored the experience of patients with chronic LBP in different socio-cultural environments. The aim of this study was to describe the experience of patients with chronic LBP in Spain and Brazil. Methods A qualitative phenomenology approach was implemented. Chronic LBP patients from the University Hospital of Salamanca (Spain), and/or Federal University of São Carlos (Brazil) were included, using purposeful sampling. Data were collected from 22 Spanish and 26 Brazilian patients during in-depth interviews and using researchers’ field notes and patients' personal diaries and letters. A thematic analysis was performed and the guidelines for reporting qualitative research were applied. Results Forty-eight patients with a mean age of 50.7 years (SD: ± 13.1 years) were included in the study. The themes identified included: a) ways of perceiving and expressing pain—the participants focused constantly on their pain and anything outside it was considered secondary; b) the socio-familial environment as a modulator of pain—most participants stated that no one was able to understand the pain they were experiencing; c) religion as a modulator of pain—all Brazilian patients stated that religious belief affected the experience of pain; and d) socio-economic and educational status as a modulator of pain—the study reported that economic factors influenced the experience of pain. Conclusions The influences of LBP can be determined based on the how a patient defines pain. Religion can be considered as a possible mechanism for patients to manage pain and as a form of solace.