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  • Articles  (6,262)
  • Articles: DFG German National Licenses  (6,262)
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  • 1995-1999  (6,262)
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  • Articles  (6,262)
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  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 106 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We have studied the stomatal response in relation to the xylem-derived abscisic acid (ABA) accumulation in sunflower leaves. When ABA was introduced into detached leaves of the sunflower through xylem flux, stomatal conductance was regulated, water flux was changed as a result and at the same time the xylem-derived ABA was metabolised in the leaves. We computed the xylem-derived ABA accumulation in the leaves as a function of time by taking into account the variation of ABA flux into the leaves (the product of water flux and ABA concentration) and a continuing ABA metabolism. We found that ABA accumulation was rapid during an initial lag phase, much slowed down during the decreasing phase of stomatal conductance, but still substantial when stomatal conductance reached a new stable state. The results show a poor link between the kinetics of ABA-induced stomatal closure and the xylem-derived ABA accumulation. Xylem-derived ABA was metabolised rapidly in the leaves. Tetcyclacis, as an inhibitor, substantially inhibited this process. Two hours after ABA was fed into a leaf, about 70% of the fed ABA was metabolised, but when tetcyclacis was added into the feeding solution, less than 30% of ABA was metabolised, even after 24 h of incubation. The inhibition of ABA metabolism by tetcyclacis did not lead to more stomatal closure, which was still concentration-dependent. Since the accumulation of xylem-derived ABA was enhanced substantially by the presence of tetcyclacis, these results strongly indicate that stomata mainly respond to the prevailing ABA concentration in the xylem stream, rather than to the accumulated amount of xylem-derived ABA in the leaves.
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  • 2
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 106 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Enzymatic digestion of the cell wall of Brassica napus hypocotyls gave a heterogeneous suspension of protoplasts with the cortical microtubules (CMTs) randomly organised or CMTs organised in parallel. The effect of variable g-influences has been tested on CMT organisation. In contrast to the 1 g-protoplasts, which reorganised the CMTs into parallel arrays during the 96 h test period, the frequency of randomly-oriented CMTs in the protoplasts exposed to simulated weightlessness (0 g) on a 2-D clinostat increased significantly during the same period. The opposite effect was obtained when the protoplasts were exposed to hyper-g (7 or 10 g), where the reorganisation of the CMTs into parallel arrays was accelerated compared to the 1 and 0 g-protoplasts. These results indicate that a unidirectional gravity force is a necessity for the reorganisation of CMTs in protoplasts to parallel arrays and that CMTs act as responding elements that are able to sense different levels of gravity. Besides the inability of the protoplasts to reorganise the CMTs into parallel arrays, the quantity of CMTs in the individual protoplast decreased during 4 days of simulated weightlessness, both compared to the CMTs quantity in the protoplasts immediately after isolation and compared to the 1 g- and hyper-g-protoplasts after 24 and 48 h of g-exposure. The size of the protoplasts was also affected by the g-exposure. Protoplasts exposed to simulated 0 g increased significantly after 24 and 48 h, whereas the 1 g- and 10 g-protoplasts maintained the same size during the 48 h test period.
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  • 3
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 105 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The role of mitochondrial respiration in optimizing photosynthesis was assessed in mesophyll protoplasts of pea (Pisum sativum L., cv. Arkel) by using low concentrations of oligomycin (an inhibitor of oxidative phosphorylation), antimycin A (inhibits cytochrome pathway of electron transport) and salicylhydroxamic acid (SHAM, an inhibitor of alternative oxidase). All three compounds decreased the rate of photosynthetic O2 evolution in mesophyll protoplasts, but did not affect chloroplast photosynthesis. The inhibition of photosynthesis by these mitochondrial inhibitors was stronger at optimal CO2 (1.0 mM NaHCO3) than that at limiting CO2 (0.1 mM NaHCO3). We conclude that mitochondrial metabolism through both cytochrome and alternative pathways is essential for optimizing photosynthesis at limiting as well as at optimal CO2. The ratios of ATP to ADP in whole protoplast extracts were hardly affected, despite the marked decrease in their photosynthetic rates by SHAM. Similarly, the decrease in the ATP/ADP ratio by oligomycin or antimycin A was more pronounced at limiting CO2 than at optimal CO2. The mitochondrial oxidative electron transport, through both cytochrome and alternative pathways, therefore akppears to be more important than oxidative phosphorylation in optimizing photosynthesis, particularly at limiting CO2 (when ATP demand is expected to be low). Our results also confirm that the alternative pathway has a significant role in contributing to the cellular ATP, when the cytochrome pathway is limited.
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  • 4
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 105 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Recent studies have suggested that Ca2+/calmodulin (CaM) or CaM-like proteins may be involved in blue light (BL)-dependent proton pumping in guard cells. As the increase in cytosolic concentration of Ca2+ is required for the activation of CaM and CaM-like proteins, the origin of the Ca2+ was investigated by measuring BL-dependent proton pumping with various treatments using guard cell protoplasts (GCPs) from Vicia faba. BL-dependent proton pumping was affected neither by Ca2+ channel blockers nor by changes of Ca2+ concentration in the medium used for the GCPs. Addition of Ca2+ ionophores and an agonist to GCPs did not induce proton pumping. However, BL-dependent proton pumping was inhibited by 10 mM caffeine, which releases Ca2+ from the intracellular stores, and by 10 μM 2,5-di-(tert-butyl)-1,4-benzohydroquinone (BHQ) and 10 μM cyclopiazonic acid (CPA), inhibitors of Ca2+-ATPase in the sarcoplasmic and endoplasmic reticulum (ER). By contrast, the inhibitions were not observed by 10 μM thapsigargin, an inhibitor of animal ER-type Ca2+-ATPase. The inhibitions by caffeine and BHQ were reversible. Light-dependent stomatal opening in the epidermis of Vicia was inhibited by caffeine, BHQ, and CPA. From these results, we conclude that the Ca2+ thought to be required for BL-dependent proton pumping may originate from intracellular Ca2+ stores, most likely from ER in guard cells, and that this origin of Ca2+ may generate a stimulus-specific Ca2+ signal for stomatal opening.
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  • 5
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 105 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Plant aspartic proteinases have been characterized from seeds, flowers and leaves of a number of different species. The enzymes are generally either monomeric or heterodimeric, containing two peptides processed from the same precursor protein. The plant enzymes, like their mammalian and microbial counterparts, are active at acidic pH and inhibited by a class specific inhibitor pepstatin A. Plant aspartic proteinases are generally either secreted or targeted to the vacuolar/protein storage body compartment. The primary sequences of many of these enzymes have been determined and are very homologous with each other as well as with enzymes from mammalian and microbial origins. Plant aspartic proteinases, however, have a very unique plant specific region, which is not found in mammalian, microbial, or viral aspartic proteinases. The function of this region has not been elucidated. A role for these plant enzymes in protein processing or degradation has been proposed, however, more studies are required to confirm their in vivo functions. Recent intriguing results suggest possible roles for these enzymes in programmed cell-death of tissues and in pathogen resistance.
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  • 6
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 105 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In shoots of the garden pea (Pisum sativum L.), the main bioactive gibberellin (GA) is GA1, which is synthesised from GA20 by 3β-hydroxylation. Gibberellin A20 is produced from GA19, as part of the process known as GA 20-oxidation. Because these steps are thought to be negatively regulated by GA1, we compared the metabolism of labelled GA19 and GA20 in mutants deficient in GA1, with that observed in isogenic wild-type (WT) plants. There was a large and specific increase in the 3β-hydroxylation of labelled GA20 in the GA1-deficient (dwarf) mutants, compared with the WT. Metabolism experiments did not provide convincing evidence for feedback regulation of 20-oxidation, possibly because GA19 akppears to be metabolised rapidly, even in WT pea shoots. Both 3β-hydroxylase and 20-oxidase transcript levels were markedly higher in the mutants than in isogenic WT lines. The results sukpport previous suggestions that both biosynthetic steps are feedback-regulated by GA1 in pea.
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  • 7
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 105 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An overview is given of electrical events that occur in plant chloroplasts in association with their energization and subsequent photosynthetic performance. Special emphasis is given to the measuring techniques, in particular application of patch-clamp methods, which enable comparison of light-induced photocurrent and -potential kinetics of the thylakoid with kinetics of changes in chlorophyll fluorescence yield.
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  • 8
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 107 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase (PFP; EC 2.7.1.90) and ATP: d-fructose-6-phosphate 1-phosphotransferase (PFK; EC 2.7.1.11) activities were determined in sugarcane varieties differing in sucrose content. For this purpose, activities were measured in those internodes where the maximum rate of sucrose accumulation occurs. The specific activity of internodal PFP varied significantly between the sugarcane varieties and was inversely correlated with the sucrose content. There was also a highly significant inverse correlation between PFP and sucrose content in a segregating F1 population. PFK activity was comparable to, or lower than, PFP activity and no relationship was evident between PFK activity and sucrose content. In all tissues investigated, the fructose 2,6-bisphosphate levels were probably sufficient to ensure full activation of PFP. The levels of PFP activity appear to be controlled by the expression of the β-subunit of the protein. The molecular mass of the PFP subunit polypeptide(s) was approximately 63 kDa. There was an inverse correlation between sucrose content and the partitioning of radiolabel into respiration in internodal tissue slices labelled with [U-14C]glucose across 3 sugarcane lines. The estimated flux of carbon into respiration correlated directly with PFP activity.
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  • 9
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 107 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Twenty-one durum wheat genotypes originating from different geographic areas were grown during 3 successive years. The trials were characterised by different precipitation regimes. Carbon isotope discrimination (Δ), carbon content (CC) and ash content (ma) were assessed in the flag leaf during anthesis, then in the kernel at full maturity. Differences between the 3 years, due to water availability, were noted for Δ, ma, CC and yield. Genotypic differences were also noted within each year for all the traits studied. Some genotypes from the Middle East exhibited higher flag leaf and kernel Δ than those originating from the West of the Mediterranean basin. The kernel Δ was strongly correlated with grain yield (GY). The leaf Δ correlated with GY only under strong water limitation and with biomass production (BP) in favourable water conditions. For the flag leaf, Δ was correlated with ma and with CC. Silicon content was then assessed in the flag leaf and in the kernel on a subset of 10 genotypes differing in their Δ values. Strong positive correlations were noted between silicon content and Δ and ma for the flag leaf. However, no clear relationship was found between silicon content and GY. The results obtained in this study confirm the validity of kernel Δ as a predictive criterion for GY under water stress and suggest the possible use of kernel ma as an alternative criterion to select genotypes with higher water stress tolerance.
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  • 10
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 107 (1999), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In order to clarify the effect of abscisic acid (ABA) on anaerobic tolerance in alfalfa (Medicago sativa L.), the seedlings were subjected to anaerobic stress after pretreatment with ABA. At concentrations〉 1 μM, ABA pretreatment increased the root viability of the seedlings to anaerobic stress and the viability increased with increasing ABA doses. At 100 μM ABA, the viability was 2.5-fold greater as compared with that of control seedlings. Roots of the seedlings rapidly lost ATP under the anaerobic stress; however, the decrease in ATP was much slower in the ABA-pretreated seedlings than the control seedlings. At 12 h after onset of the stress, ATP concentrations in the roots of 100 and 10 μM ABA-pretreated seedlings were 2.7- and 2.0-fold that of the control seedlings, respectively. During the period of ABA pretreatment under aerobic condition, ABA increased alcohol dehydrogenase (ADH, EC 1.1.1.1) activity in the roots until 12 h and then leveled off. The maximum ADH activities were 4.3- and 2.8-fold that in the roots of the control seedlings for 100 and 10 μM ABA-pretreated seedlings, respectively. After being subjected to the anaerobic stress, both ADH activities in the roots of the ABA-pretreated and the control seedlings increased but the differences in their activity remained. These results suggest that ABA pretreatment may maintain ATP level due to induction of ADH activity, which may be one of the causes of increasing anaerobic tolerance in the seedlings.
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