ISSN:
0741-0581
Keywords:
Enzyme-gold
;
Cytochemistry
;
Nucleic acids
;
Elastin
;
Collagen
;
Glycogen
;
Xylans
;
Chitins
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
The enzyme-gold postembedding approach has been introduced recently in the field of cytochemistry for the ultrastructural localization of macromolecules. This technique is based on the affinity properties existing between an enzyme and its substrate. The possibility of detecting substrate molecules by applying enzyme-gold complexes has been established. The present review deals with the development and the technical approach of this method. Various applications are reported for the demonstration of the reliability of the technique that yields results of high specificity and resolution. In addition, some technical problems and limitations particular to this method are reported and discussed.
Additional Material:
30 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060010405
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