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  • Articles  (8)
  • Articles: DFG German National Licenses  (8)
  • Latest Papers from Table of Contents or Articles in Press
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  • Articles  (8)
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  • Articles: DFG German National Licenses  (8)
  • Latest Papers from Table of Contents or Articles in Press
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  • Springer  (8)
  • American Chemical Society (ACS)
  • Cambridge University Press
  • Nature Publishing Group
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 143 (1973), S. 117-133 
    ISSN: 1432-0878
    Keywords: Nervous system ; Ctenophores ; Intra-mesogleal nervous system ; Synapses ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural evidence is given of the occurrence of nervous elements in the mesoglea of Ctenophores based on the presence of the typical synapses of this phylum. In Beroids, nervous fibers from the ectodermal nerve-net cross the epithelial basal membrane and run through the mesoglea; they are devoid of any ensheathing cell. These neurites build highly differentiated synapses upon the muscles and upon peculiar cells, tentatively named mesenchymal cells. In Cydippids, nerve fibers and nerve cell-bodies have been observed in the mesoglea of the tentacles. The mesogleal core of each tentacle contains mesenchymal cells and a thick strand of neurons and neurites, forming a kind of elongated ganglion. Neurites of either the axial neurones or the epithelial nerve-net neurones form numerous radial nerve strands across the tentacular muscles. Interneural, neuro-muscular and neuro-mesenchymal junctions are very frequent in the tentacle. As far as the organization of the mesoglea is concerned, the Ctenophora thus appear closer to Turbellaria than to Cnidaria.
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  • 2
    ISSN: 1432-0878
    Keywords: Nervous system ; Stick insect ; Blood-brain barrier ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fat body sheath which surrounds the ventral nerve cord of the adult stick insect, Carausius morosus, is absent in the hatchling. Since the haemolymph sodium ion compositions of the two stages are similar, it is suggested that the fat body sheath is not a site of a blood-brain barrier dealing with extra-axonal regulation of sodium. Similarities of ultrastructure in connectives of adults and hatchlings are demonstrated, and tight junctions are shown to occur in perineural cells of both stages. The possible significance of this to the blood-brain barrier of this insect is discussed.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 141 (1973), S. 301-317 
    ISSN: 1432-0878
    Keywords: Cnidaria ; Nervous system ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nervous system of juvenile and adult Ceriantheopsis americanus has been examined with the electron microscope. The nervous system is exclusively ectodermal, forming a plexus of fibres lying between the epithelium and the muscle layer. The plexus consists of three types of nerve fibre that vary in diameter from 0.1 to 20 μ, and a fourth type of fibre of uncertain nature. The reticulum, previously thought to be the ectodermal nervous system, is redescribed as the peduncles of epithelial supporting cells. Intraneural and neuromuscular synapses are described. About 97% of the interneural are polarized. The unpolarized synapses are a variety not previously described, consisting of a series of polarized zones, for which the name multipolarized synapse is proposed. Structurally, the interneural synapses are more complex than those previously seen in the Cnidaria. Presynaptic projections, periodic cleft densities, and a postsynaptic web are described. By contrast, the neuromuscular synapses bear no membrane specializations. On the basis of observed synaptic interactions of its components a preliminary model of the functional organization of the nervous system is proposed.
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  • 4
    ISSN: 1432-0878
    Keywords: Dense core vesicles ; Ultrastructure ; Mollusc ; Nervous system ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy of the cerebro-visceral connectives of Anodonta cygnea shows numerous long rows of dense core vesicles within the smaller axons of the connectives. These vesicles are often linked together by bridges of electron-dense material reinforced by one or more “arms”. The use of the tilting stage on the electron microscope, which allows the bridges to be examined from different angles, confirms the presence of these vesicular connections. Sub-cellular fractionation of the cerebro-visceral connectives was carried out to study the nature of these bridged dense core vesicles. In the negatively stained vesicle fraction two or more dense core vesicles are joined in a row by connections similar to the vesicular bridges of the intact connective. In the fixed vesicle fraction, many of the dense core vesicles are connected by bridges but long rows of bridged vesicles are not seen. The functional significance of the vesicular bridges is discussed. Preliminary findings of this work have been reported previously (McLaughlin and Howes, 1973).
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 144 (1973), S. 339-351 
    ISSN: 1432-0878
    Keywords: Nervous system ; Tissue culture ; Sheath cells ; Sensory end-organs ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fetal spinal ganglia and spinal cord segments with adhering spinal ganglia were explanted on collagen-coated coverslips. They were investigated with enzyme histochemical methods for the existence of hydrolases and dehydrogenases up to 54 days of cultivation. Alkaline phosphatase was located in arachnoid cells and in mantle cells (satellite cells). Butyrylcholinesterase and alpha-glycerophosphate-menadione-tetrazolium reductase were found in mantle cells. Acetylcholinesterase and indoxylesterase were active in the whole neuron; acid phosphatase and alpha-naphthylacetate esterase were restricted to the perikarya. During the process of cell differentiation in vitro alkaline phosphatase decreased in mantle cells. Acid phosphatase became diminished distinctly in spinal ganglion cells. The other neuronal enzymatic activities remained unaltered during the whole period of cultivation. Proliferated Schwann cells were conspicuous by their activity for butyrylcholinesterase. In newly formed myelin sheaths arylsulphatase was active. Lactate dehydrogenase was contained in the perineurium which had developed. Cultures of long duration could contain cytological formations which were reminiscent of sensory end-organs with respect to their enzyme patterns. The enzyme activities of nervous tissues in vitro in their approximation to the situation in situ are discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 137 (1973), S. 21-36 
    ISSN: 1432-0878
    Keywords: Nervous system ; Glycera convoluta (annelids) ; Biogenic amines ; Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'étude par la méthode histochimique de fluorescence de Falck, du ganglion cérébroïde ét de la chaine nerveuse ventrale de Glycera convoluta K. a permis la mise en évidence de péricaryons et de fibres présentant une fluorescence jaune et verte. La nature et le métabolisme des amines mises en évidence histochimiquement ont été étudiées par des techniques biochimiques. La présence d'adrénaline et de 5-hydroxytryptamine a pu être établie avec certitude. Deux autres amines identifiées chromatographiquement comme étant de la dopamine et de la noradrénaline présentent cependant des caractéristiques spectrofluorimétriques légèrement différentes de ces deux substances. La rétention de la noradrénaline et de la dopamine étudiée par des techniques radiochimiques s'est montrée plus longue que dans le canal déférent du Rat. Les composants lipidiques, protéolipidiques et protéiques étudiés chromatographiquement sont apparus assez différents de ceux rencontrés chez les Mammifères. Des données récentes ont montré l'importance de l'arrangement spatial des groupements fonctionnels dans la molécule du médiateur par rapport à la (ou aux) protéine (s) lors de la fixation de la noradrénaline dans les fibres adrénergiques des Mammifères. Aussi peut on estimer que les différences observées entre les caractéres spectrofluorimétriques des catécholamines de G. convoluta et du Rat pourraient tenir plus à la forme de capture et de stockage des amines qu'à une différence profonde de leur nature chimique.
    Notes: Summary The supra-oesophageal ganglion and ventral nerve cord of Glycera convoluta K. were studied with the fluorescence method of Falck. Many perikarya and fibers show a green and yellow fluorescence. The nature and metabolism of the observed biogenic amines were studied by biochemical methods. Epinephrine and 5-hydroxytryptamine were certainly present. Two other amines show chromatographic characters of dopamine and norepinephrine, but exhibit a slight difference in their fluorimetric spectra with these two catecholamines. Radiochemical methods have shown that the retention of norepinephrine and dopamine is longer in the ventral nerve cord of G. convoluta than in the vas deferens of the rat. The lipids, proteolipids and proteins of G. convoluta were different from those found in mammals as demonstrated by chromatographic analysis. Recent findings have shown the importance of the spatial arrangement of active groups in the molecule of transmitter for its retention by the protein matrix in the adrenergic fibers of mammals. The differences found by spectrofluorimetry and by measurements of the time of retention between catecholamines of rat and G. convoluta can be explained by differences in the nature of the protein matrix or by the spatial arrangement of active groups in the transmitter molecule.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 137 (1973), S. 313-329 
    ISSN: 1432-0878
    Keywords: Nervous system ; Limulus polyphemus ; Abdominal ganglion ; Neurons ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of the first abdominal ganglion of Limulus polyphemus (L.) was examined by reconstructions from stained serial sections. This ganglion, one of a chain of eight which form the ventral cord, is bilaterally symmetrical and shows the typical invertebrate division into two zones, cellular rind and fibrous core. The core of the ganglion comprises two major longitudinal tracts which are continuous with the connectives between the ganglia and four areas of regional neuropile which occur at the roots of the nerves developed from the ganglion. Minor tracts and neuropile are present. The rind is divided into several cell groups: U-shaped lobes around the nerve roots, a lateral lobe between the nerve roots and two cell layers along the ventral aspect. Six cell types are recognized: encapsulated unipolar cells, secretory unipolar cells (two types), encapsulated bipolar cells, “neuroglandular” bipolar cells and small non-encapsulated cells.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 137 (1973), S. 223-250 
    ISSN: 1432-0878
    Keywords: Nervous system ; Ctenophores ; Nerve-net, Nervous concentration ; Synapses ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A subectodermal nerve-net is demonstrated in ten species of Ctenophores by means of either Methylene Blue vital staining or silver impregnation. There is no evidence of subendodermal nerve-net. The ectodermal nerve-net displays a characteristic polygonal pattern which is thought to result from morphogenetic events. Both bipolar and tripolar neurones occur. Three types of concentrations of nervous tissue are described: a) a high accumulation of nervous perikarya at the aboral sensory pole; b) a noticeable densification of the net along the eight meridional ciliary strands of every species, and around the lips of Beroidea; c) in the Cydippids Pleurobrachia and Hormiphora two thick strands of fibers and neurones interconnecting the aboral organ and the tentacles: the tentacular nerves. Ultrastructural evidence is given for the presence of nervous elements under and amidst ectodermal epithelial cells. Three features allow the recognition and characterization of neurites and neurones: a) numerous clear and/or granulated vesicles; b) microtubules in variable amounts; c) frequent and highly differentiated synaptic contacts. The very peculiar arrangement of the presynaptic elements is observed in all the species of Ctenophores so far studied. These synaptic contacts suggest chemical transmission in the nerve-net. On the basis of ultrastructural evidence the author refutes the nervous nature previously attributed to the ciliated cells of the meridional grooves. A parallel is drawn between nervous systems of Cnidaria and Ctenophora.
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