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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 113 (1971), S. 13-28 
    ISSN: 1432-0878
    Keywords: Sensory receptors ; Insects ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The antennae of Scolytus multistriatus were examined with light and scanning and transmitting-electron microscopy to determine the distributions, types and structures of sense organs. Four types of sensilla were found: (1) sensilla chaetica, a singly-innervated receptor; (2) sensilla basiconica, Type A, a short, thin-walled, multiple-innervated receptor; (3) sensilla basiconica, Type B, a long, thin-walled multiple-innervated receptor; and (4) sensilla trichodea, short, thick-walled, multiple-innervated receptors. The positioning of dendrite(s) with regard to pore tubules is elaborated. Evidence for lack of sensory axon fusion is presented.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 112 (1971), S. 97-119 
    ISSN: 1432-0878
    Keywords: Insects ; Epicuticle secretion ; Golgi system ; Lysosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les modifications cytologiques des cellules épidermiques sécrétant la cuticule sternale et pleurale de l'adulte deT. molitor sont étudiées pendant la formation et l'évolution de l'épicuticule externe et interne. L'épicuticule externe et interne sont sécrétées de la même façon par condensation de matériel diffus au-dessus de l'apex des microvillosités. Un cycle hétérolytique est mis en évidence par l'emploi de peroxydase injectée dans l'espace exuvial: la peroxydase est captée par de grosses vésicules revêtues et incorporée dans des corps multivésiculaires apicaux. Par comptage des différentes populations de vésicules, des variations nettes dans l'intensité de la pinocytose sont mises en évidence. Ces variations semblent en rapport non pas avec la digestion de l'ancienne cuticule mais avec les différences de nature et de structure des composants cuticulaires. L'hypothèse du contrôle de la sécrétion épidermique par les phénomènes de pinocytose énoncée par Locke (1969) est confirmée. Des vésicules d'origine golgiennes sont secrétées surtout après la formation de l'épicuticule et ne sont donc pas à l'origine du matériel épicuticulaire, mais il est possible qu'elles jouent un rôle dans l'évolution ultérieure des structures épicuticulaires.
    Notes: Summary The cytological modifications of the epidermal cells secreting sternal and pleural cuticle of adultTenebrio are studied, specially during the formation and evolution of the outer and inner epicuticle. Both layers are secreted by condensation of diffuse material just above the epidermal microvilli. There is no peculiar phase without microvilli when inner epicuticle is formed. During the secretion of pre-ecdysial cuticle, the epidermal cell is characterized by an heterolytic process which is demonstrated by peroxidase injection into the ecdysial space: horseradish peroxidase is absorbed by large pinocytic coated vesicles which discharge their content into multivesicular bodies, after the loss of their coat. The intensity of pinocytosis is studied by vesicles counts at different stages of the pupal life. These counts show a pinocytic peak when the inner epicuticle is formed. When the epicuticular barrier is completely secreted, exogenous peroxidase is no more absorbed, but morphological study and counts show that pinocytosis still occurs. This demonstrates that epidermis cannot absorb macromolecules arising from the digested old cuticle since digestion occurs after the completion of adult epicuticle. Locke's hypothesis about the “control of the extracellular subcuticular environment” by pinocytosis is confirmed. The Golgi complexes are involved in lytic process with their small coated vesicles and in secretion of cuticular material with their large dense secretory vesicles, but according to vesicle counts, the secretory vesicles are not concerned in epicuticle formation since their apparition is posterior to the deposition of the inner epicuticle.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 114 (1971), S. 61-72 
    ISSN: 1432-0878
    Keywords: Corpora cardiaca ; Neurosecretion ; Insects ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les corpora cardiaca de l'adulte de Locusta migratoria sont formés de deux régions bien individualisées ce qui nous a permis de reconnaître la sécrétion propre des différents types de neurosécrétion. Dans la région nerveuse, nous distinguons par la taille des grains trois types de neurosécrétion dense classique et un quatrième type d'aspect clair. Dans la région »propre« non nerveuse, les cellules ont des caractères nettement endocriniens et sont mélangées à un seul type d'axones neurosécréteurs.
    Notes: Summary The corpora cardiaca of adult Locusta migratoria consist of two well separated areas, a fact which permits the differentiation between intrinsic and extrinsic neurosecretory material. In the neural area three types of electron dense “classical” neurosecretory granules, and a fourth more lucent type can be distinguished according to size. In the non-neural “glandular” area typical endocrine cells mingle with only one type of neurosecretory axons.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 118 (1971), S. 333-368 
    ISSN: 1432-0878
    Keywords: Malpighian tubule ; Insects ; Carausius ; Microscopy, electron ; Biological transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Structural features of the principal, urine-secreting cells (type 1 cells) of the Malpighian tubules of Carausius are de scribedquantitatively and discussed in relation to possible mechanisms of water and solute transport. Mitochondria are arranged in two bands of about equal volume near to the basal and apical surfaces, suggesting active processes occur at both surfaces. Basal infoldings and apical microvilli which greatly amplify the cell surface are probably primarily devices to increase the passive permeability of the tissue to solutes. They do not provide functionally significant standing-osmotic-gradients. The extensive endoplasmic reticulum is locally differentiated into several components and ramifies between the infoldings and along microvilli but probably is not an intracellular conduit for the majority of urinary constituents. Vesicles and stages in their formation or liberation are observed both basally and apically although they probably do not contribute significantly to transcellular transport. At present it remains a problem to satisfactorily account for observations that the urine of Carausius can be hypotonic.
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  • 5
    ISSN: 1432-0878
    Keywords: Chordotonal organ ; Insects ; Head appendages ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les organes chordotonaux présents dans les différentes pièces céphaliques de la larve du Speophyes peuvent être classés en deux catégories. La première catégorie regroupe les récepteurs scolopidiaux de l'antenne, du labium et du palpe maxillaire. On peut les comparer au scolopidium de l'organe tympanique du Criquet décrit par Gray (1960). La deuxième catégorie comprend les récepteurs scolopidiaux de la mandibule et de la lacinia: ils sont du amphinématique. Le sensille scolopidial de la galea représente un type intermédiaire. Nous signalons l'importance des structures de soutien et de fixation, qui doivent permettre une bonne transmission de toutes les déformations et tensions subies par le tégument. Nous discutons du rôle joué par la ≪gap junction≫ qui unit les deux dendrites dans les scolopidium. de la deuxième catégorie. Enfin nous essayons d'établir des hypothèses sur le fonctionnement des scolopidium.
    Notes: Summary The chordotonal organs located in the various head appendages of the Speophyes larva, can be divided into two classes. The scolopidial receptors of the antenna, the labium and the palpus maxillae belong to the first class. They can be compared to the scolopidium of the locust tympanic organ described by Gray.—The second class contains the scolopidial receptors of the mandible and the lacinia: their type is amphinematic. The scolopidial sensilla of the galea represents an intermediate type. We demonstrate many supporting and fixation structures which probably allow a good transmission of all the deformations and strains affecting the tegument. The function of the gap junction which connects the two dendrites in the scolopidia of the second class is discussed. Finally we try to formulate hypothesis of the functioning of scolopidia.
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  • 6
    ISSN: 1432-0878
    Keywords: Symbiotes ; Hereditary ; Insects ; Types ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'examen au microscope électronique des symbiontes transmis par l'œuf chez les Homoptères, montre des types très divers: levures, bactéries plus ou moins régressées et des formes énigmatiques ressemblant parfois à des globules de sécrétion. Toutefois, les diverses catégories de symbiontes décrites auparavant en microscopie photonique ne sont pas toujours retrouvées. Il n'est pas possible de caractériser un genre par ses symbiontes.
    Notes: Summary Electron microscope study of symbiotes hereditarily transmitted through the eggs of in Homoptera, shows very different types: yeasts, more or less degenerated bacteria and enigmatical forms looking in some way like drops of secretion. Yet the numerous distinct species of symbiotes described in some cases by light microscopists are not always found again. It is not possible to distinguish a genus by its symbiotes.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 122 (1971), S. 210-226 
    ISSN: 1432-0878
    Keywords: Insects ; Cercus ; Mechanoreceptors ; Fine structur ; Ecdysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Mit einsetzender Apolysis öffnet sich der äußere Liquorraum der Sensillen in den Exuvialraum. Die cuticularen Scheiden und die in ihnen enthaltenen Ciliarstrukturen werden verlängert. Die cuticularen Scheiden grenzen die Sinnescilien gegen die Exuvialflüssigkeit ab. 2. Über die cuticularen Scheiden stehen die alten rezeptorischen Apparate mit den Sinneszellen bis zur Ecdysis in Verbindung. Bei der Bildung der neuen Cuticula wird das Cuticulamaterial um sie herum abgelagert. Dadurch entstehen Kanäle, die wir Häutungskanäle nennen. Sie bleiben auch nach der Ecdysis erhalten, wenn die cuticularen Scheiden und die Sinnescilien hier abgerissen sind. 3. Der Häutungskanal der Faden- und Keulenhaare liegt über der Haarbasis, der der campaniformen Sensillen in der Mitte der cuticularen Kuppel. 4. Im Sinnescilium wird bereits vor der Ecdysis unter der neuen Cuticula ein zweiter, neuer Tubularkörper gebildet. Der alte Tubularkörper geht bei der Häutung verloren. Dadurch können die Sensillen wahrscheinlich bis zur Häutung (alter Tubularkörper!) und sofort nach der Häutung (neuer Tubularkörper!) Reize wahrnehmen. 5. Mechanorezeptorische Sinneshaare und campaniforme Sensillen sind homologe Bildungen. Die innere Hüllzelle (H 1) legt die cuticulare Scheide an. Die mittlere Hüllzelle (H 2) ist die trichogene und die äußere Hüllzelle (H 3) die tormogene Zelle.
    Notes: Summary 1. With the onset of apolysis the external extracellular space of the sensilla opens into the exuvial space. The cuticular sheaths and the ciliary structures become lengthened. The cuticular sheaths delimit and protect the modified cilia against the exuvial fluid. 2. The old receptor apparatus stays in contact with the sensory cells above the cuticular sheath until ecdysis. In the formation of the new cuticle, the cuticular material is deposited around the cuticular sheath, thereby forming channels, called the ecdysial canals. These also remain intact after the cuticular sheath and the modified cilium become torn away. 3. The ecdysial canal of the filiform and club-shaped hairs is situated above the base of the hair; the ecdysial canal of the campaniform sensilla is in the middle of the cuticular cap. 4. A second and new tubular body is already formed under the new cuticle in the modified cilium before ecdysis. The old tubular body disappears during ecdysis. The sensilla therefore can probably perceive stimuli until the time of ecdysis through the old tubular body and there-after through the new tubular body. 5. Mechanoreceptive sensory hairs and campaniform sensilla are homologous structures. The internal enveloping cell (H 1) lays down the cuticular sheath. The middle enveloping cell (H 2) is the trichogen cell, and the external one (H 3) is the tormogen cell.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 122 (1971), S. 283-300 
    ISSN: 1432-0878
    Keywords: Paraventricular nucleus ; Rat ; Synthesis of hormones ; Acid phosphatase ; Osmium impregnation ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural features of the paraventricular neurones of the non-treated rat are presented comparing them with those of the supraoptic neurones. No striking differences are seen between the general electron microscopic characteristics of the paraventricular and supraoptic neurones. The importance of adequate fixation to obtain good preservation of the neurones is emphasized, since inadequate fixation can cause e.g. artefactual appearance of “dark neurones”. The previously presented classification of the neurosecretory neurones into two categories (e.g. “light and dark neurones”) on the basis of the number of ribosomes is not considered justifiable, since their number can vary to a very great extent even within a single cell. The synthesis of neurosecretory products in the paraventricular neurones obviously follows the general mode of the synthesis of secretory proteins: ribosomes—RER—Golgi complex—secretory vesicles. On the basis of the localization of heavy metal deposits after osmium impregnation and demonstration of acid phosphatase the Golgi complex of the paraventricular neurones is found to be polarized. The direction of the polarity is discussed. The substructures of the dense cores of the neurosecretory granules and of the contents of the lysosomal dense bodies are nearly identical. Therefore it is considered impossible to determine positively the nature of the dark condensed material within the Golgi complex. The characteristics of the immature neurosecretory granules and the possibility of releasing neurosecretory products into the cytoplasm already within the perikarya are speculated.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 123 (1972), S. 47-54 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Exocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Elektronenmikroskopische Untersuchungen an der Neurohypophyse von Ratte und Forelle ergeben, daß sich eine Exocytose von Elementargranula an den Endigungen der neurosekretorischen Fasern nur selten abspielt. Es wird daher angenommen, daß die Abgabe von Hormonen in der Neurohypophyse in der Regel nach dem Muster des „membrane-release“ abläuft. 2. Die Exocytose wird nicht durch eine unmittelbare tangentiale Fusion der Membran des Elementargranulums mit dem Plasmalemm der Nervenendigung (Axolemm) eingeleitet. Vor allem bei Anwendung eines Goniometertisches wird erkennbar, daß vor der Exocytose zwischen Axolemm und Membran des Granulums eine Verbindung in Gestalt eines Stieles entsteht. Die Länge dieses Verbindungsstückes entspricht etwa 2 Axolemmdicken. An der Basis des Stiels im Axolemm tritt das Stoma auf, durch das der Inhalt des Granulums bzw. dieses selbst das Axonende verläßt. 3. Die Herkunft kleiner membrannaher Vesikel (Durchmesser 500 Å) in den Endigungen neurosekretorischer Nervenfasern in der Neurohypophyse konnte nicht geklärt werden. Anzeichen einer kompensatorischen Endocytose im Sinne von Nagasawa, Douglas und Schulz (1970) wurden nicht beobachtet.
    Notes: Summary 1. Electron microscopical investigations of the neurohypophysis in rat and trout reveal that exocytosis of neurosecretory elementary granules from the nerve endings occurs only rarely. The authors are of the opinion that hormone release in the neural lobe follows mainly the “membrane-release” pattern. 2. Exocytosis is not performed by tangential fusion of the elementary granule membrane and the plasmalemma of the nerve ending (axolemma). Administering the goniometer technique one can observe the appearance of a stalk-like structure connecting the two membranes. The basis of the stalk in the axolemma corresponds to the site of the stoma through which the core of the vesicle leaves the nerve ending. 3. The mechanism of the origin of small clear vesicles (diameter 500 Å approx.) near the axolemma of the neurosecretory terminal has not been elucidated. The authors did not observe equivalents of a compensatory endocytosis in the vicinity of granules released by exocytosis.
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  • 10
    ISSN: 1432-0878
    Keywords: Hypophysis ; Chondrichthyens ; Embryology ; Neurosecretion ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'identification et la répartition des cellules endocrines dans l'ébauche adénohypophysaire de Scyllium canicula, et la chronologie de la différenciation cellulaire et de l'histogenèse de cette glande ont fait l'objet de ce travail. Plus nettement encore que chez l'adulte on démontre qu'il y a quatre populations de cellules hormonogènes, localisées chacune dans un lobe différent. Les grains de sécrétion des cellules du lobe rostral sont glycoprotidiques et ont un diamètre modal, de 170 nm, ceux du lobe médian, de la même taille, sont protidiques ou polypeptidiques. Les gros granules du lobe neuro-intermédiaire (diamètre modal 260 nm) sont glycoprotidiques, les petits granules du lobe ventral (diamètre modal 120 nm), protidiques au polypeptidiques. Au stade 10 mm, les cellules de la poche de Rathke sont différenciées de celles de l'épithélium stomodéal par la présence d'inclusions énigmatiques, les ≪godd bodies≫ de Schechter. Au stade 35 mm les cellules des noyaux préoptiques et du tuber comportent des grains de neurosécrétion, de même diamètre, variant autour de 100 nm. A ce même stade, des granules apparaissent simultanément dans les quatre lobes de l'hypophyse, notamment dans le lobe neuro-intermédiaire où aucune fibre neurosécrétrice n'est encore décelée. L'innervation directe des cellules endocrines de ce lobe ne s'effectue qu'au stade 45 mm et s'accompagne de modifications de la taille des granules. La fin du développement embryonnaire se caractérise par une charge de plus en plus importante des cellules endocrines, en granules.
    Notes: Summary The identification and distribution of endocrine cells in embryonic adenohypophysis of Seyllium canicula, and chronology of cellular differentiation and histogenesis of this gland have been investigated in this work. More clearly than in the adult, the presence of four populations of endocrine cells, each one located in a different lobe, is demonstrated. Secretion granules of rostral lobe cells are glycoprotidic, their modal diameter is 170 nm; median lobe ones, of same dimensions, are protidic or polypeptidic. The nemo-intermediate lobe big granules (modal diameter: 260 nm) are glycoprotidic, the ventral lobe small granules (modal diameter: 120 nm), protidic or polypeptidic. At stage 10 mm, Rathke's pouch cells are recognized from stomodeal epithelium by enigmatic inclusions, the “odd bodies” of Schechter. At stage 35 mm, preoptic and tuberal nucleus cells include neurosecretory granules of same diameter varying about 100 nm. At this same stage, granules appear simultaneously in the four hypophyseal lobes, including the neuro-intermediate lobe where no neurosecretory fiber is present. Direct innervation of endocrine cells of this lobe is accomplished at stage 45 mm and is accompanied by changes of granules dimensions. The end of embryonic development is characterized by increasing granular load of endocrine cells.
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