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  • Articles  (53)
  • Articles: DFG German National Licenses  (53)
  • Latest Papers from Table of Contents or Articles in Press
  • Scanning electron microscopy  (28)
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  • Springer  (53)
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  • Medicine  (53)
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  • Articles  (53)
Source
  • Articles: DFG German National Licenses  (53)
  • Latest Papers from Table of Contents or Articles in Press
Publisher
  • Springer  (53)
  • American Chemical Society (ACS)
  • American Society of Hematology
  • Wiley-Blackwell  (6)
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  • 2020-2024
  • 2015-2019
  • 1985-1989  (34)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Lasers in medical science 1 (1986), S. 111-115 
    ISSN: 1435-604X
    Keywords: Argon laser ; Arterial microanastomosis ; Scanning electron microscopy ; Vessel re-endothelialization ; Collagenous fusion ; Vessel repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Description / Table of Contents: Résumé Anastomoses micro-artérielles au laser argon: étude en microscopie électronique à balayage. Les auteurs réalisent au laser Argon (Coherent 900) une anastomose carotidienne terminoterminale sur une série de 50 rats Wistar de poids moyen de 260 g. Les impacts laser (en moyenne 19) sont de 300 mW de puissance et d'une durée de 5 s chacun, avec un point de focalisation de 150 μm de diamètre. On réalise sur 13 spécimens un examen en microscopie électronique à balayage. L'impact laser induit sur la paroi artérielle une lésion de 100 μm de large avec une légère nécrose de coagulation de la media et de l'adventice. La ligne de suture est re-endothélialisée dès le quatrième jour, alors qu'une fusion du collagène est observée dans les couches sousendothéliales. L'arrangement longitudinal des cellules endothéliales est retrouvé dés le dixième jour. A long terme, un réseau collagénique serré assure la résistance de la media et un endothélium normal recouvre la ligne de ‘soudure’. Les complications tel que lâchage ou anéurysmes doivent être attribuées aux inconvénients techniques du début de l'expérimentation.
    Notes: Abstract A carotid end-to-end anastomosis was performed in 50 Wistar rats (mean weight 260 g) by means of a Coherent 900 argon laser. Laser pulses (average 19) of 300 mW power and 5 s exposure time were used, the beam being focused to form a spot of 150 μm diameter. From day 0 to day 210, 13 specimens underwent scanning electron microscope examination. The results show that the laser impact produces a wall injury of 100 μm in width with some coagulative necrosis of the media and adventitia. The line of anastomosis became re-endothelialized within four days, at which time collagen fusion was observed in the subendothelial layers. The longitudinal arrangement of the endothelial cells was restored by day 10. In the long term, a thick collagenous meshwork maintained the strength of the media, while normal endothelium covered the anastomosis. Complications such as disruption and aneurysm formation were attributed to technical problems.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1220-1222 
    ISSN: 1420-9071
    Keywords: Hibernating chipmunks ; myocardium ; plateau potential ; slow inward current ; strontium ; 4-aminopyridine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the myocardium of nonhibernating chipmunks, replacing external Ca by Sr markedly prolongs the action potential plateau with an increase in contraction, while in preparations from hibernating animals this procedure inhibits both responses. Pretreatment with 4-aminopyridine causes a prolongation of the action potential plateau by Sr in hibernating animals.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 148 (1974), S. 69-82 
    ISSN: 1432-0878
    Keywords: Ultimobranchial glands ; Anuran larvae ; Metamorphosis ; Ultrastructure ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of ultimobranchial (UB) gland cells from Rana temporaria larvae 48 h after hatching until the completion of metamorphosis is described. A single UB cell type is present, believed to be the characteristic C cell, in which secretory granules are first detectable in 8 day post-hatching larvae. These secretory granules show an intimate association with lipid droplets. Unusual membranous and crystalloid inclusions, which may represent yolk platelets, are found in UB glands of very small larvae. The significance of a range of UB organelles is discussed and some scanning electron micrographs presented. This report is believed to be the first published ultrastructural and scanning electron microscope study of larval anuran UB glands.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 150 (1974), S. 389-397 
    ISSN: 1432-0878
    Keywords: Choroid plexus ; Human fetus ; Modifications during development ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural organization of the human fetal choroid plexus was assessed with scanning electron microscopy. The membranous modifications of choroidal ependymal cells differ remarkably between 11 and 20 weeks of intrauterine development and suggest a variable functional capacity at different times of ontogenesis. Based upon existing data coupled with the ultra-architectural organization of cilia, clavate and linear microvilli are seen with scanning electron microscopy, a multiple functional role is hypothesized for choroidal ependymal cells.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 153 (1974), S. 151-165 
    ISSN: 1432-0878
    Keywords: Taste buds ; Fishes ; Chemoreceptors and Mechanoreceptors ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Within the epithelium of the head gut of the sword-tail (Xiphophorus helleri Heckel) there are three types of taste buds that can be identified with certainty in the scanning electron microscope. The breathing valves, which are situated behind the mouth region, bear relatively high epidermal papillae in which type I taste buds can be found. The middle region of the head gut is mostly occupied by low epidermal papillae containing type II taste buds. Type III taste buds are present within the metabranchial head gut; they never rise above the normal level of the epithelium. Taste buds of each type show two kinds of receptor villi within their receptor areas: tall villi (about 1,5×0,5 μm) and small villi (about 0.5×0.2 μm). A few tall villi belonging to solitary chemosensory cells are situated between the superficial epithelial cells of the tongue. In addition, the surface of the epidermal cells shows a delicate microridge system.—It is postulated that the taste buds of different types serve different functions: Taste buds within epidermal papillae may act both as chemoreceptors, and mechanoreceptors. Taste buds that never rise above the normal level of the epithelium perhaps act predominantly as chemoreceptors.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 148 (1974), S. 83-96 
    ISSN: 1432-0878
    Keywords: Sharpey fibre bone ; Collagen orientation ; Mineralization ; Mammals ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sharpey fibre bone specimens from cranial sutures and muscle attachment sites of rhesus monkeys and rats, and tooth sockets of several mammals (man, rhesus monkey and rat specimens mainly) were fixed, and either dried by critical point drying or freeze drying, or made anorganic with 1,2 ethane diamine or sodium hypochlorite, washed and airdried. The bone was examined by scanning electron microscopy. The packing density of the extrinsic fibres affected their cross sectional shape, the well separated fibres being the most uniform. Long parallel rows and groups of extrinsic fibres were observed. The fibre units that made up the Sharpey fibre bundles were sometimes distinct and mineralized as units. Mineralization was generally similar to the varied patterns encountered in root cementum. The diameters of the extrinsic fibres were similar at all sites and in all species; those in socket bone differed little from those in cementum. The intrinsic fibre orientation, although constant over fairly large areas, showed no consistent relation to the direction of entry of the extrinsic fibres. We would like to thank Mrs. E. Bailey and Mr. P. S. Reynolds for technical assistance.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 147 (1974), S. 149-156 
    ISSN: 1432-0878
    Keywords: Sensorysur face ; Tentacles ; Sea anemones ; Corals ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Examination of the sensory surface of the tentacles of sea anemones and corals prepared by the Freon critical-point method has revealed three primary structures: ciliary cones consisting of a cilium surrounded by a cluster of shorter stereocilia, single long cilia, and microvilli. The ciliary cones occur on, and the single cilia and microvilli occur both on and between, the nematocyst- and spirocyst-bearing regions of the tentacles. The similarity of the anthozoan ciliary cones to the ciliary sensory structures in the lateral line, and the auditory and vestibular organs of vertebrates is noted and the possible functional significance of the coelenterate structures is discussed.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 147 (1974), S. 163-167 
    ISSN: 1432-0878
    Keywords: Articular cartilage ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Samples of articular cartilage from four different human joints were obtained at surgery. Serial scanning electron micrographs taken at a magnification of 1000× were used to reconstruct an 0.25 mm2 area of articular surface. Within these given areas both normal and degenerated portions were seen. This study supports the concept that the surface morphology of articular cartilage varies from joint to joint and from area to area within a joint. This information should be useful for the interpretation of light and electron micrographs, as well as histochemical and biochemical data.
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  • 9
    ISSN: 1432-0878
    Keywords: Ciliated cells ; Mucous membranes ; Trachea ; Heat exposure ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mucociliary system of the respiratory tract is easily influenced by temperature — and humidity variations in the surrounding air. A series of functional in vitro experiments on heat-exposed rabbit trachea has been performed. The present investigation deals with the corresponding morphological changes of tracheal surface structures after exposure to temperatures between 40°C and 50°C and at a constant humidity of approximately 90 per cent. The following pathological findings were observed at a temperature of 42°C: 1. Fusion of cilia in their distal ends after 20–45 min., 2. appearance of submembranous vesicles without visible matrix after 45–165 min., 3. disappearance of vesicles and a definite deterioration of ciliary structures after 165 min.
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  • 10
    ISSN: 1432-0878
    Keywords: Saccus vasculosus ; Rainbow trout ; Osmoregulation ; Infundibular recess ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Injection of a saccus vasculosus homogenate from sea water adapted rainbow trout into the third brain ventricle of fresh-water rainbow trout increased the survival time of the latter in sea water of high salinity. This indicated an active role of saccus vasculosus in osmoregulation. Autoradiographic analysis of brains from injected fish that had received a saccus vasculosus homogenate from sea water adapted specimens, in which the coronet cells of saccus vasculosus had previously been labelled with 3H-glucoseamine, revealed actual incorporation of labelled material into brain cells, especially in the hypothalamic region. The intracellular distribution of labelled material in the coronet cells of rainbow trout labelled with 3H-glucoseamine strongly indicates that such material represents a secretory product; it is therefore probable that the observed incorporation of label into brain cells of injected fish actually reflects normal processing of material secreted from the coronet cells. In the scanning electron microscope, the inside wall of the third brain ventricle of the rainbow trout was in many areas found to be covered with cilia. These apparently serve to keep liquor in circulation. The observation, both in injected and control animals, of free globules among these cilia supports the idea of an intra-ventricular transport of detached coronet cell material to the infundibular recess, as indicated by the autoradiographic analysis.
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