ALBERT

Description: Micro-Raman spectroscopy has been used on adult bivalve shells to investigate organic and inorganic shell components but has not yet been applied to bivalve larvae. It is known that the organic matrix of larval shells contains pigments, but less is known about the presence or source of these molecules in larvae. We investigated Raman spectra of seven species of bivalve larvae to assess the types of pigments present in shells of each species and how the ratio of inorganic : organic material changes in a dorso-ventral direction. In laboratory experiments, we reared larvae of three clam species in waters containing different organic signatures to determine if larvae incorporated compounds from source waters into their shells. We found differences in spectra and pigments between most species but found less intraspecific differences. A neural network classifier for Raman spectra classified five out of seven species with greater than 85% accuracy. There were slight differences between the amount and type of pigment present along the shell, with the prodissoconch I and shell margin areas being the most variable. Raman spectra of 1-day-old larvae were found to be differentiable when larvae were reared in waters with different organic signatures. With micro-Raman spectroscopy, it may be possible to identify some unknown species in the wild and trace their natal origins, which could enhance identification accuracy of bivalve larvae and ultimately aid management and restoration efforts.

Description: Tephra provides regional chronostratigraphical marker horizons that can link different climate archives with highly needed accuracy and precision. The results presented in this work exemplify, however, that the intermittent storage of tephra in ice sheets and during its subsequent iceberg transport, especially during glacial stages, constitutes a potential source of serious error for the application of tephrochronology to Nordic Seas and North Atlantic sediment archives. The peak shard concentration of the rhyolitic component of the North Atlantic Ash Zone II (NAAZ-II) tephra complex, often used to correlate marine and ice core records in Marine Isotope Stage (MIS) 3, is shown to lag the eruption event by ca. 100–400 years in some North Atlantic and Norwegian Sea cores. While still allowing for a correlation of archives on millennial timescales, this time delay in deposition is a major obstacle when addressing the lead–lag relationship on short timescales (years to centuries). A precise and accurate determination of lead–lag relationships between archives recording different parts of the climate system is crucial in order to test hypotheses about the processes leading to abrupt climate change and to evaluate results from climate models. Copyright # 2011 John Wiley & Sons, Ltd.

Description: Using the fault plane mechanisms of the shallow earthquakes occurring along the Hellenic arc and the extent of the intermediate seismic belt, we make a quantitative estimate of the relative motion occurring between the Hellenic arc and the adjacent sea floor. This estimate is then used to evaluate the deformation in the Aegean area and to reconstruct the pattern of motion over the Eastern Mediterranean region for the last 13 m.y. It is shown that this pattern is compatible with the neotectonic and seismicity studies in Aegea. We then discuss the dynamics of the area and propose that, since Serravallian-Tortonian time, Aegea has been spreading gravitationally in front of the southwestward advancing Turkey. The reasons for this gravitational spreading are discussed.

Description: The granule floatation is a serious issue of the anammox (anaerobic ammonium oxidation) process when high loading rates are applied that results in instability or even system collapse. The present study reports the granule floatation in an anammox reactor when high loading rates were applied. The comparison of enlarged photos taken for the settling and floating granules showed that the two kinds of granules both contained macroscopic gas pockets accounting for 11 +/- 14% of total volume. The settling granules had gas tunnels that could release the gas bubbles, while the floating granules did not. The presence of gas bubbles enclosed in the gas pockets led to the small density of 979.2 +/- 15.8 mg L(-1) and flotation of anammox granules. Consequently, the flotation caused washout of anammox granules and the deterioration of anammox process (volumetric removal rate decreased from 4.00 to 2.46 kg N m(-3) d(-1)). The collection of floating granules, breaking them into small pieces and then returning to the anammox reactor proved an effective control strategy. The volumetric removal rate was finally up to 16.5 kg N m(-3) d(-1) after the control strategy was put into use.

Description: The spatial variability in the mix of species making up Cold-water coral reef communities is not well known. In this study abundances of a selection of megafauna (Lophelia pertusa, Madrepora oculata, Paragorgia arborea, Primnoa resedaeformis, Mycale lingua, Geodia baretti, Acesta excavata and fish) were quantified throughout 9 manned submersible video transects from 3 reef complexes (Røst Reef, Sotbakken Reef and Traena Reef) on the Norwegian margin. Substrate type (coral structure, rubble, exposed hardground or soft sediment) was also recorded. Variations in the densities of these fauna (with respect to both reef complex and substrate type) were investigated, with spatial covariance between species assessed. For the majority of fauna investigated, densities varied by both reef and substrate. Spatial covariance indicated that some species may be utilising similar habitat niches, but that minor environmental differences may favour colonisation by one or other at a particular reef. Fish densities were generally higher in regions with biogenic substrate (coral structure and coral rubble substrates) than in areas of soft or hardground substrate. Further, fish were more abundant at the northerly Sotbakken Reef at time of study than elsewhere. Community structure varied by reef, and therefore management plans aimed at maintaining the biodiversity of reef ecosystems on the Norwegian margin should take this lack of homogeneity into account.

Description: The Cap de Creus continental shelf and Cap de Creus canyon are located in the southern most sector of the Gulf of Lions, in the northwestern Mediterranean. The Cap de Creus continental shelf contains sandy and muddy sediments and an abrupt morpho­ logy, with rocky outcrops, relict bioherms, erosive features, and planar bedforms. The Cap de Creus canyon breaches the shelf at a depth of 110 m and denotes a marked dif­ ference in the morphology between the northern and the southern flank, reflecting a different depositional regime. The most common substrates correspond to coarse and medium sands (28%) and silty sediments (40%). The most common megabenthic assemblages of the shelf correspond to the communities of "offshore detritic" (31.95%) and "coastal terrigenous muds" (36.99%), mostly dominated by sea pens, alcyonaceans, and ceriantharians. The northern flank of the Cap de Creus canyon is predominantly depositional, whereas the southern flank is erosional. Rocky outcrops provide the sub­ stratum for cold­water coral (CWC) communities' development, in which the white coral Madrepora oculata is the most abundant species.

Description: INTRODUCTION: Lichens are self-sustaining partnerships comprising fungi as shape-forming partners for their enclosed symbiotic algae. They produce a tremendous diversity of metabolites (1050 metabolites described so far). OBJECTIVES: A comparison of metabolic profiles in nine lichen species belonging to three genera (Lichina, Collema and Roccella) by using an optimised extraction protocol, determination of the fragmentation pathway and the in situ localisation for major compounds in Roccella species. METHODS: Chemical analysis was performed using a complementary study combining a Taguchi experimental design with qualitative analysis by high-performance liquid chromatography coupled with mass spectrometry techniques. RESULTS: Optimal conditions to obtain the best total extraction yield were determined as follows: mortar grinding to a fine powder, two successive extractions, solid:liquid ratio (2:60) and 700 rpm stirring. Qualitative analysis of the metabolite profiling of these nine species extracted with the optimised method was corroborated using MS and MS/MS approaches. Nine main compounds were identified: 1 β-orcinol, 2 orsellinic acid, 3 putative choline sulphate, 4 roccellic acid, 5 montagnetol, 6 lecanoric acid, 7 erythrin, 8 lepraric acid and 9 acetylportentol, and several other compounds were reported. Identification was performed using the m/z ratio, fragmentation pathway and/or after isolation by NMR analysis. The variation of the metabolite profile in differently organised parts of two Roccella species suggests a specific role of major compounds in developmental stages of this symbiotic association. CONCLUSION: Metabolic profiles represent specific chemical species and depend on the extraction conditions, the kind of the photobiont partner and the in situ localisation of major compounds.

Description: Brackish coastal groundwater is enriched in Ra, which is transported to surface waters via submarine groundwater discharge (SGD). The Ra activity of the SGD end-member is influenced by a variety of environmental factors including salinity, pH, and isotope half-life. In the York River estuary (YRE), 223Ra, 224Ra, and 226Ra were measured in surface water and shallow groundwater across a range of salinities and additional Ra sources quantified (desorption and diffusion from sediments, input from tidal marshes). The Ra budget of the estuary indicated a major source of Ra that could only be satisfied by SGD. The apparent Ra flux was combined with groundwater Ra end-member activity to estimate SGD volume fluxes of 5–178 L m− 2 d− 1. Each isotope exhibited a different seasonal pattern, with significantly higher 224Ra flux during summer than winter, lower 226Ra SGD flux during summer than winter, and no seasonal differences in 223Ra SGD flux. However, the SGD 224Ra end-member activity varied with seasonal pore water salinity fluctuations, indicating end-member control on seasonal 224Ra flux. Each Ra isotope suggested a different SGD volume flux, indicating that different nuclide regeneration rates may respond to and reflect different flow mechanisms in the subterranean estuary. This work indicates that volume fluxes estimated using geochemical tracers are sensitive to SGD end-member variations and end-member variability must be well-characterized for reliable SGD flux estimates.

Description: Solid-solution partitioning of Ra determines the dissolved Ra composition of porewater in marine sands. Therefore, sorption controls also influence the endmember concentration of Ra in submarine groundwater discharge (SGD). Ra is widely used as a tracer of SGD, and constraining sorption controls in permeable sands is necessary to evaluate spatial and temporal variation in Ra groundwater activities. This work presents Ra distribution coefficients measured in seawater (salinity 35) for some common solid sorbents as well as different solution compositions relevant to permeable marine sands and the subterranean estuary. There was a strong correlation of Ra distribution coefficient (Kd = solid-phase Ra/solution Ra) with surface area for size-fractionated sediments (log Kd (L/g) = 0.77 [log S.A. (m2/g)] + 0.73; r2 = 0.76). Ra sorption showed no direct relationship with solid-phase Fe or Mn content of the sands, although removal of visible surficial oxide coatings with dilute acid reduced Kd by a factor of 2 to 3. Synthetic Fe-oxides showed nearly two orders of magnitude difference in Ra sorption. Ferrihydrite had the highest Ra sorption coefficient at 1535 ± 410 L kg− 1, followed by lepidocrocite (174 ± 21 L kg− 1), hematite (75 ± 17 L kg− 1), and goethite (20 ± 8 L kg− 1). A marked increase in Ra adsorption was observed with increasing pH, with the sorption edge of natural sands falling within the pH range of 5–8. The extent of Ra sorption at a given pH varied among different substrates. No effect of dissolved Fe was observed on Ra partitioning. A large increase in Ra Kd was evident with increasing Ba concentration when seawater contained sulfate, opposite the effect that would be expected for sorption competition. No effect of Ba concentration was observed when sulfate was excluded from the ASW, indicating that barite precipitation caused the Kd increase. There was no clear effect of temperature on Ra sorption between 2 and 60 °C. Results of this study show that minor solid-phase components increase the Ra sorption capacity of bulk sands and buffer the dissolved Ra concentration (i.e., the SGD endmember). Solution controls on Ra sorption have the potential to greatly alter the Ra composition of discharging groundwater. Given that high-salinity, high-pH conditions probably prevail in porewater below the sediment–water interface, the actual SGD Ra endmember may be less variable than suggested by compilations that include groundwater from deep and fresh groundwater. Highlights ► Variable Ra partitioning to size-fractionated and diverse sediments was primarily controlled by specific surface area. ► Ra displayed pH-dependent sorption to sands, with a sorption edge between pH 5 and 8. ► No effect on Ra partitioning was observed for temperature or competition by dissolved Fe and Ba.

Description: Submarine groundwater discharge (SGD) into a shallow lagoon on the west coast of Mauritius Island (Flic-en-Flac) was investigated using radioactive (3H, 222Rn, 223Ra, 224Ra, 226Ra, 228Ra) and stable (2H, 18O) isotopes and nutrients. SGD intercomparison exercises were carried out to validate the various approaches used to measure SGD including radium and radon measurements, seepage rate measurements using manual and automated meters, sediment bulk conductivity and salinity surveys. SGD measurements using benthic chambers placed on the floor of the Flic-en-Flac Lagoon showed discharge rates up to 500 cm/day. Large variability in SGD was observed over distances of a few meters, which were attributed to different geomorphological features. Deployments of automated seepage meters captured the spatial and temporal variability of SGD with a mean seepage rate of 10 cm/day. The stable isotopic composition of submarine waters was characterized by significant variability and heavy isotope enrichment and was used to predict the contribution of fresh terrestrially derived groundwater to SGD (range from a few % to almost 100%). The integrated SGD flux, estimated from seepage meters placed parallel to the shoreline, was 35 m3/m day, which was in reasonable agreement with results obtained from a hydrologic water balance calculation (26 m3/m day). SGD calculated from the radon inventory method using in situ radon measurements were between 5 and 56 m3/m per day. Low concentrations of radium isotopes observed in the lagoon water reflected the low abundance of U and Th in the basalt that makes up the island. High SGD rates contribute to high nutrients loading to the lagoon, potentially leading to eutrophication. Each of the applied methods yielded unique information about the character and magnitude of SGD. The results of the intercomparison studies have resulted a better understanding of groundwater–seawater interactions in coastal regions. Such information is an important pre-requisite for the protection and management of coastal freshwater resources. Highlights ► Large fluctuations in SGD fluxes from 0 to 360 cm/day were observed. ► The integrated shoreline SGD fluxes were between 5 and 56 m3/m day. ► The groundwater contribution in SGD varied from a few % to almost 100%. ► The observed high SGD rates contributed to high nutrients loading to the lagoon.