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Rearranged c-mos locus in a MOPC 21 murine myeloma cell line and its persistence in hybridomas

Abstract

Studies of a number of normal and carcinogen-transformed murine cell lines, and a variety of murine tissues, have indicated that, in contrast to several other cellular oncogenes, the oncogene c-mos gene is usually transcriptionally silent1,2. The recent report by Rechavi et al.3 indicating that in the mouse myeloma XRPC24 originally induced by pristane (2,6,10-14-tetramethylpentadecane) the c-mos gene is rearranged and transcriptionally active, and that it can transform murine fibro-blasts in a transfection assay, is therefore of considerable interest. Here we show that the c-mos locus has undergone a similar rearrangement, and is also transcriptionally active, in the cell line P3-X63-Ag8-653, a derivative of the mouse myeloma MOPC 21 which was induced by mineral oil4,5. This line is widely used for making hybridomas that synthesize monoclonal antibodies6,7. We also demonstrate that the rearranged c-mos sequence is maintained in three different hybridomas derived by fusion of this cell line with normal murine spleen lymphocytes, suggesting that it may play a role in the continuous growth and/or constitutive immunoglobulin production by these hybridomas.

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Gattoni-Celli, S., Hsiao, WL. & Weinstein, I. Rearranged c-mos locus in a MOPC 21 murine myeloma cell line and its persistence in hybridomas. Nature 306, 795–796 (1983). https://doi.org/10.1038/306795a0

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