Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Recombinant vaccinia virus primes and stimulates influenza haemagglutinin-specific cytotoxic T cells

Nature volume 311, pages 578–579 (1984)Cite this article

Abstract

The ability of vaccinia virus to accept and express cloned genes1–3 encoding immunologically important proteins of unrelated viruses4–9 and malarial parasites10 has suggested a novel approach to the development of live vaccines. Vaccinia virus recombinants retain infectivity and stimulate synthesis of specific antibodies to the cloned gene products in vaccinated animals. Moreover, animals inoculated with recombinants expressing the influenza virus haemagglutinin (HA)7, the hepatitis B virus surface antigen11, and type 1 herpesvirus glycoprotein D8 were protected against subsequent challenge with the corresponding virus. For maximal effectiveness, vaccines should produce cellular as well as humoral immunity. We now report that a vaccinia virus recombinant, expressing the influenza HA, primes and stimulates a specific murine cytotoxic T-lymphocyte (CTL) response. Histocompatible cells infected with this recombinant also serve as targets for CTLs. These properties make vaccinia virus a unique tool for studying cell-mediated immunity and enhance the attractiveness of this vector for production of live vaccines.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Panicali, D. & Paoletti, E. Proc. natn. Acad. Sci. U.S.A. 79, 4927–4931 (1982).

    Article  ADS  CAS  Google Scholar 

  2. Mackett, M., Smith, G. L. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 79, 7415–7419 (1982).

    Article  ADS  CAS  Google Scholar 

  3. Mackett, M., Smith, G. L. & Moss, B. J. Virol. 49, 857–864 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Smith, G. L., Mackett, M. & Moss, B. Nature 302, 490–495 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Smith, G. L., Mackett, M. & Moss, B. in UCLA Symp. molec. cell. Biol. new Ser. (eds Hamer, D. & Rosenberg, M.) 543–554 (Liss, New York, 1983).

    Google Scholar 

  6. Panicali, D., Davis, S. W., Weinberg, R. L. & Paoletti, E. Proc. natn. Acad. Sci. U.S.A. 80, 5364–5368 (1983).

    Article  ADS  CAS  Google Scholar 

  7. Smith, G. L., Murphy, B. R. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 80, 7155–7159 (1983).

    Article  ADS  CAS  Google Scholar 

  8. Paoletti, E., Lipinskas, B. R., Samsonoff, C., Mercer, S. & Panicali, D. Proc. natn. Acad. Sci. U.S.A. 81, 193–197 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Smith, G. L., Mackett, M., Murphy, B. R. & Moss, B. in Modern Approaches to Vaccines (eds Chanock, R. & Lerner, L.) (Cold Spring Harbor Laboratory, New York, in the press).

  10. Smith, G. L., Godson, B. N., Nussenzweig, V., Nussenzweig, R. S. & Moss, B. Science 224, 397–399 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Moss, B., Smith, G. L., Gerin, J. L. & Purcell, R. H. Nature 311, 67–69 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Braciale, T. J., Braciale, U. L., Henkel, T. J., Sambrook, J. S. & Gething, M. J. exp. Med. 159, 341–354 (1984).

    Article  CAS  PubMed  Google Scholar 

  13. Winter, G., Fields, S. & Brownlee, G. G. Nature 292, 72–75 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Smith, G. L. & Moss, B. Gene 25, 21–28 (1983).

    Article  CAS  PubMed  Google Scholar 

  15. Zinkernagel, R. M. & Doherty, P. C. Adv. Immun. 27, 51–77 (1979).

    Article  CAS  PubMed  Google Scholar 

  16. Yap, K. L., Ada, G. L. & McKenzie, I. F. C. Nature 273, 238–239 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Lin, Y. & Askonas, B. A. J. exp. Med. 154, 225–234 (1981).

    Article  CAS  PubMed  Google Scholar 

  18. Yewdell, J. W., Frank, E. & Gerhard, W. J. Immun. 126, 1814–1819 (1981).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Wistar Institute, 36th Street at Spruce, Philadelphia, Pennsylvania, 19104, USA

    J. R. Bennink, J. W. Yewdell & C. Moller

  2. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, 20205, USA

    G. L. Smith & B. Moss

Authors
  1. J. R. Bennink
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. W. Yewdell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. L. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Moller
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Moss
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bennink, J., Yewdell, J., Smith, G. et al. Recombinant vaccinia virus primes and stimulates influenza haemagglutinin-specific cytotoxic T cells. Nature 311, 578–579 (1984). https://doi.org/10.1038/311578a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/311578a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing