Abstract
In vitro transcription studies of cloned messenger RNA-coding genes have yielded considerable information regarding the sequence elements and protein factors involved in transcription initiation and RNA processing (reviewed in ref. 1). Fractionation of whole-cell2, S-100 protein3 and nuclear extracts4 reveals the existence of both general class II5–9 and gene-specific8,10,11 transcription initiation factors. Because the soluble in vitro transcription systems prepared from cells in culture are largely nonspecific for the origin of the template DNA1,12, they are highly suited to searching for tissue-specific and gene-specific transcription regulatory factors. In the experiments reported here, we have added a nuclear extract prepared from human erythroleukaemia-like cells (K562, which can be induced to synthesize ε- and γ-globin mRNA and protein13,14) to several deproteinized DNA templates, and monitored transcription levels in a HeLa cell-free transcription system2. The K562 nuclear extract enhanced transcription of β-, ε- and γ-globin genes by as much as 30-fold compared with control non-globin templates. These results suggest the presence of a globin gene regulatory factor in erythroleukaemia cell nuclei.
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References
Heintz, N. & Roeder, R. G. Genet. Engng 4, 57–89 (1982).
Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 3855 (1980).
Weil, P. A., Luse, D. S., Segall, J. & Roeder, R. G. Cell 18, 469–484 (1979).
Dignam, J. D., Lebowitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1489 (1983).
Matsui, T., Segall, J., Weil, P. A. & Roeder, R. G. J. biol. Chem. 255, 11992–11996 (1980).
Samuels, M., Fire, A. & Sharp, P. A. J. biol. Chem. 253, 14419–14427 (1982).
Davidson, B. L., Egly, J. M., Mulvihill, E. R. & Chambon, P. Nature 301, 680–686 (1983).
Dynan, W. S. & Tjian, R. Cell 32, 669–680 (1983).
Parker, C. S. & Topol, J. Cell 36, 357–369 (1984).
Parker, C. S. & Topol, J. Cell 37, 273–283 (1984).
Heintz, N. & Roeder, R. G. Proc. natn. Acad. Sci. U.S.A. 81, 2713–2727 (1984).
Manley, J. L., Fire, A., Samuels, M. & Sharp, P. A. Meth. Enzym. 101, 568 (1983).
Anderson, L. C., Jokinen, M. & Gahmberg, C. G. Nature 278, 354 (1979).
Rutherford, T. R., Clegg, J. B. & Weatherall, D. J. Nature 280, 164 (1979).
Rall, L. B., Standring, D. N., Laub, O. & Rutter, W. J. Molec. cell. Biol. 3, 1766 (1983).
Read, G. S. & Summers, W. S. Proc. natn. Acad. Sci. U.S.A. 79, 5215 (1982).
Charney, P. & Maniatis, T. Science 220, 1281–1283 (1983).
Weintraub, H., Beug, H., Groudine, M. & Graf, T. Cell 28, 931–940 (1982).
Groudine, M., Kohwi-Shigematsu, T., Gelinas, R., Stamatoyannopoulos, G. & Papayannopoulou, T. Proc. natn. Acad. Sci. U.S.A. 80, 7551–7555 (1983).
Emerson, B. M., Lewis, C. D. & Felsenfeld, G. Cell 41, 21–30 (1985).
Proudfoot, N. J., Shander, M., Manley, J. L., Gefter, M. L. & Maniatis, T. Science 209, 1329–1336 (1980).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201 (1980).
Goldberg, D. A. Proc. natn. Acad. Sci. U.S.A. 77, 5794 (1980).
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Bazett-Jones, D., Yeckel, M. & Gottesfeld, J. Nuclear extracts from globin-synthesizing cells enhance globin transcription in vitro. Nature 317, 824–828 (1985). https://doi.org/10.1038/317824a0
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DOI: https://doi.org/10.1038/317824a0
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