Abstract
THE single-headed myosins called myosin-I were first isolated from the protozoan Acanthamoeba1 and subsequently identified in other cells (reviewed in ref. 2). We previously reported evidence that myosin-I is responsible for the movement of membranes, extracted from Acanthamoeba, along actin filaments in vitro3. Here we show for the first time that myosin-I can bind directly to NaOH-extracted membranes isolated from Acanthamoeba and to vesicles of pure lipids with an affinity sufficient for extensive binding in the cell. Membrane-bound myosin-I may provide a mechanism for many cellular movements previously thought to involve filamentous myosin-II.
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References
Pollard, T. D. & Korn, E. D. J. biol. Chem. 248, 4682–4690 (1973).
Korn, E. D. & Hammer III, J. A. A. Rev. biophys. Chem. 17, 23–45 (1988).
Adams, R. J. & Pollard, T. D. Nature 322, 754–756 (1986).
Gadasi, H. & Korn, E. D. Nature 286, 452–456 (1980).
Hagen, S. J., Kiehart, D. P., Kaiser, D. A. & Pollard, T. D. J. Cell Biol. 103, 2121–2128 (1986).
Adams, R. J. & Pollard, T. D. in Cell Movement Vol. 2 (eds Warner, F. D. & McIntosh, J. R.) 3–12 (Alan R. Liss, New York, 1988).
Mooseker, M. S. & Tilney, L. G. J. Cell Biol. 67, 725–743 (1975).
Carboni, J. M. et al. J. Cell Biol. 107, 1749–1757 (1988).
Luna, E. G., Fowler, V. M., Swanson, J., Branton, D. & Taylor, D. L. J. Cell Biol. 88, 396–409 (1981).
Steck, T. L. & Yu, J. J. supramol. Struct. 1, 220–232 (1973).
Korn, E. D. & Wright, P. L. J. biol. Chem. 248, 439–447 (1973).
Pollard, T. D. & Korn, E. D. J. biol. Chem. 248, 448–450 (1973).
Clarke, B. J., Hohman, T. C. & Bowers, B. J. Protozool. 35, 408–413 (1988).
Lynch, T. J. et al. J. biol. Chem. 261, 17156–17162 (1986).
Jung, G., Korn, E. D. & Hammer, J. A. Proc. natn. Acad. Sci. U.S.A. 84, 6720–6724 (1987).
Fukui, Y., Lynch, T. J., Brzeska, H. & Korn, E. D. J. Cell Biol. 107, 471a (1988).
Knecht, D. A. & Loomis, W. F. Science 236, 1081–1087 (1987).
de Lozanne, A. & Spudich, J. A. Science 236, 1086–1091 (1987).
Honer, B., Citi, S., Kendrick-Jones, J. & Jockusch, B. M. J. Cell Biol. 107, 2181–2189 (1988).
Sinard, J. & Pollard, T. D. Cell Motil. Cytoskel. 12, 47–52 (1989).
Pollard, T. D. & Korn, E. D. J. biol. Chem. 248, 4691–4697 (1973).
Albanesi, J. P., Fujisaki, H. & Korn, E. D. J. biol. Chem. 260, 11174–11179 (1985).
Laemmli, U. K. Nature 227, 680–685 (1970).
Kielley, W. W. & Harrington, W. F. 41, 401–421 (1960).
Margossian, S. S. & Lowey, S. Meth. Enzym. 85, 55–71 (1982).
Bradford, M. Anal. Biochem. 193, 248–254 (1976).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).
Bolton, A. E. & Hunter, W. M. Biochem. J. 133, 529–539 (1973).
Ulsamer, A. G., Wright, P. L., Wetzel, M. G. & Korn, E. D. J. Cell Biol. 51, 193–215 (1971).
Smith, S. J. Science 242, 708–715 (1988).
Mitchison, T. & Kirschner, M. Neuron 1, 761–772 (1988).
Miyata, H., Bowers, B. & Korn, E. D. J. Cell Biol. (in the press).
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Adams, R., Pollard, T. Binding of myosin I to membrane lipids. Nature 340, 565–568 (1989). https://doi.org/10.1038/340565a0
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DOI: https://doi.org/10.1038/340565a0
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