Abstract
The nucleotide sequence of the 5′ end of bacteriophage Qβ RNA has been determined by a new method involving partial synthesis of the RNA in vitro. Neither of the codons commonly associated with the initiation of protein synthesis (AUG and GUG) was found before position 62. The sequence required for the initiation of synthesis of the coat protein (PuUGGCN) is not within the first 300 nucleotides from the 5′ end. A very stable hydrogen-bonded loop seems to exist close to the 5′ end of the molecule.
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References
Gussin, G. N., J. Mol. Biol., 21, 435 (1966).
Horiuchi, K., Lodish, H. F., and Zinder, N. D., Virology, 28, 438 (1966).
Garwes, D., Sillero, A., and Ochoa, S., Biochim. Biophys. Acta, 186, 166 (1969).
Weith, H. L., and Gilham, P. T., J. Amer. Chem. Soc., 89, 5473 (1967).
de Wachter, R., and Fiers, W., J. Mol. Biol., 30, 507 (1967).
de Wachter, R., and Fiers, W., Nature, 221, 233 (1969).
Bishop, D. H. L., Mills, D. R., and Spiegelman, S., Biochemistry, 7, 3744 (1968).
Dahlberg, J. E., Nature, 220, 548 (1968).
Glitz, D. G., Bradley, A., and Fraenkel-Conrat, H., Biochim. Biophys. Acta, 161, 1 (1968).
de Wachter, R., Verhassel, J. P., and Fiers, W., FEBS Lett., 1, 93 (1968).
Min Jou, W., and Fiers, W., J. Mol. Biol., 40, 187 (1969).
Adams, J., Nature, 223, 1009 (1969).
Argetsinger-Steitz, J., Cold Spring Harbor Symp. Quant. Biol., 34 (in the press).
Hindley, J., and Staples, D. H., Nature, 224, 964 (1969).
Haruna, I., and Spiegelman, S., Proc. US Nat. Acad. Sci., 54, 579 (1965).
Weissmann, C., Feix, G., Slor, H., and Pollet, R., Proc. US Nat. Acad. Sci., 57, 1870 (1967).
Feix, G., Pollet, R., and Weissmann, C., Proc. US Nat. Acad. Sci., 59, 145 (1968).
Banerjee, A. K., Kuo, C. H., and August, J. T., J. Mol. Biol., 40, 445 (1969).
Billeter, M. A., Dahlberg, J. E., Goodman, H., Hindley, J., and Weissmann, C., J. Cell. Physiol. (in the press).
Billeter, M. A., Dahlberg, J. E., Goodman, H., Hindley, J., and Weissmann, C., Cold Spring Harbor Symp. Quant. Biol., 34 (in the press).
Eoyang, L., and August, J. T., in Methods in Enzymology (edit. by Grossmann, L., and Moldave, K.), 12B, 530 (Academic Press, New York, 1968).
Franze de Fernandez, M. T., Eoyang, L., and August, J. T., Nature, 219, 588 (1968).
Pollet, R., Knolle, P., and Weissmann, C., Proc. US Nat. Acad. Sci., 58, 766 (1967).
Sanger, F., Brownlee, G. G., and Barrell, B. G., J. Mol. Biol., 13, 373 (1965).
Arima, T., Uchida, T., and Egami, F., Biochem. J., 106, 609 (1968).
Brownlee, G. G., and Sanger, F., J. Mol. Biol., 23, 337 (1967).
Bernardi, G., in Procedures in Nucleic Acid Research (edit. by Cantoni, G. L., and Davis, D. R.), 37 (Harper and Row, New York and London, 1966).
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BILLETER, M., DAHLBERG, J., GOODMAN, H. et al. Sequence of the First 175 Nucleotides from the 5′ Terminus of Qβ RNA synthesized in vitro. Nature 224, 1083–1086 (1969). https://doi.org/10.1038/2241083a0
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DOI: https://doi.org/10.1038/2241083a0
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