Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

High Frequency Haploid Production in Barley (Hordeum vulgare L.)

Abstract

HAPLOIDS have been sought and investigated in many plant species since their first identification in Datura1. The chief limitation in using them to produce homozygous lines for plant breeding programmes or genetic research has been the lack of techniques by which large numbers of haploids can be obtained from any given stock2. Reports3,4 of haploid plants obtained from anther cultures offer a potential technique and here we report our first observations on interspecific crosses, followed by embryo culture, illustrating an equally promising method for obtaining haploids in barley and perhaps other species.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Blakeslee, A. F., Belling, J., Farnham, M. E., and Berger, A. D., Science, 55, 646 (1922).

    Article  ADS  CAS  Google Scholar 

  2. Kimber, G., and Riley, R., Bot Rev., 90, 480 (1963).

    Article  Google Scholar 

  3. Niizeki, H., and Oono, K., Proc. Jap. Acad., 44, 554 (1968).

    Article  Google Scholar 

  4. Nitsch, J. P., and Nitsch, C., Scince, 163, 85 (1969).

    Article  ADS  CAS  Google Scholar 

  5. Kao, K. N., and Kasha, K. J., in Proc. Second Intern Barley Genetics Symp., July 6–11, 1969 (edit. by R. A. Nilan) (Wash. State Univ. Press, in the press).

    Google Scholar 

  6. Gamborg, O. L., Miller, R. A., and Ojima, K., Exp. Cell Res., 50, 151 (1968).

    Article  CAS  Google Scholar 

  7. Lesins, K., Canad. J. Genet. Cytol., 3, 135 (1961).

    Article  Google Scholar 

  8. Clement, W. M., Canad. J. Genet. Cytol., 5, 427 (1963).

    Article  Google Scholar 

  9. Hougas, R. S., Peloquin, S. J., and Ross, R. W., J. Hered., 49, 103 (1958).

    Article  Google Scholar 

  10. Wangenheim, K. H. V., Peloquin, S. J., and Hougas, R. S., Z. Verebung., 91, 391 (1960).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

KASHA, K., KAO, K. High Frequency Haploid Production in Barley (Hordeum vulgare L.). Nature 225, 874–876 (1970). https://doi.org/10.1038/225874a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/225874a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing