Abstract
THE RNA in picornaviruses can function directly as mRNA (ref. 1). In contrast to the great majority of cellular and virus mRNA molecules, however, a ‘cap’ structure has not been found at the 5′-end of the RNAs of polio2,3 and encephalomyocarditis (EMC) (ref. 4) virus particles or on poliovirus specific mRNA in infected cells2,3. The 5′-terminus of poliovirus mRNA extracted from polyribosomes was found to be pUp2,3 but no nucleotide could be identified at the 5′-end of the RNA in virus particles. This is because a small protein is covalently linked to the 5′-terminal nucleotide of the RNA (refs 5, 6). The RNAs of EMC virus and foot-and-mouth disease virus (FMDV) contain a tract of about 100–200 cytidylic acid residues located near the 5′-end7,8 but such a sequence was not found in poliovirus RNA (ref. 9). It was of interest, therefore, to determine whether a protein is also covalently linked to FMDV RNA and whether it is attached to the poly C tract. We show here that FMDV RNA is not capped and demonstrate the presence of a protein of molecular weight (MW) about 4,000 covalently linked to the RNA. The protein is not attached directly to the poly C tract but is probably attached to the 5′-terminus and can be removed without loss of infectivity.
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References
Baltimore, D. Bact. Rev. 35, 235–241 (1971).
Hewlett, M. J., Rose, J. K. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 73, 327–330 (1976).
Nomoto, A., Lee, Y. F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 73, 375–380 (1976).
Frisby, D., Eaton, M. & Fellner, P. Nucleic Acids Res. 3, 2771–2787 (1976).
Lee, Y. F., Nomoto, A., Detjen, B. M. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 59–63 (1977).
Flanegan, J. B., Pettersson, R. F., Ambros, V., Hewlett, M. J. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 74, 961–965 (1977).
Chumakov, K. M. & Agol, V. I. Biochem. biophys. Res. Commun. 71, 551–557 (1976).
Harris, T. J. R. & Brown, F. J. gen. Virol. 33, 493–501 (1976).
Brown, F. et al. Nature 251, 342–344 (1974).
Harris, T. J. R. & Brown, F. J. gen. Virol. 34, 87–105 (1977).
Cartwright, B., Talbot, P. & Brown, F. J. gen. Virol. 7, 267–272 (1970).
Swank, R. T. & Munkries, K. D. Analyt. Biochem. 39, 462–477 (1971).
Wild, T. F., Martin, S. J. & Brown, F. Biochem. J. 107, 395–401 (1968).
Fernandez-Munoz, R. & Lavi, U. J. Virol. 21, 820–824 (1977).
Nomoto, A., Morgan Detjen, B., Pozzatti, R. & Wimmer, E. Nature (in the press).
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SANGAR, D., ROWLANDS, D., HARRIS, T. et al. Protein covalently linked to foot-and-mouth disease virus RNA. Nature 268, 648–650 (1977). https://doi.org/10.1038/268648a0
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DOI: https://doi.org/10.1038/268648a0
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