Abstract
The RNA polymerase mutation, alt-1, affects the σ subunit and alters the in vitro selectivity of RNA polymerase to parallel the in vivo phenotype. We propose that the mutation changes the distribution of functionally distinct polymerase isomers.
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References
Perlman, R. L. & Pastan, I. J. biol. Chem. 243, 5420–5427 (1968).
Ullman, A. & Monod, J. FEBS Lett. 2, 57–60 (1968).
Zubay, G., Schwartz, D. & Beckwith, J. R. Proc. natn. Acad. Sci. U.S.A. 66, 104–110 (1969).
Eron, L. & Block, R. Proc. natn. Acad. Sci. U.S.A. 68, 1828–1832 (1971).
Crombrugghe, B. de et al. Nature new Biol. 231, 139–142 (1971).
Silverstone, M. E., Goman, M. & Scaife, J. G. Molec. gen. Genet. 118, 223–234 (1972).
Bautz, E. K. F., Bautz, F. A. & Dunn, J. J. Nature 223, 1022–1024 (1969).
Sugiura, M., Okamoto, T. & Takanami, M. Nature 225, 598–600 (1970).
Hinkle, D. C. & Chamberlin, M. J. J. molec. Biol. 70, 157–185 (1972).
Nakamura, Y., Osawa, T. & Yura, T. Proc. natn. Acad. Sci. U.S.A. 74, 1831–1835 (1977).
Harris, J. D., Martinez, I. I. & Calendar, R. Proc. natn. Acad. Sci. U.S.A. 74, 1836–1840 (1977).
Jacobson, A. & Gillespie, D. Cold Spring Harb. Symp. quant. Biol. 35, 85–93 (1970).
Georgopoulos, C. P. Proc. natn. Acad. Sci. U.S.A. 68, 2977–2981 (1971).
Panny, S. R. et al. FEBS Lett. 48, 241–245 (1974).
Gross, G., Fields, D. A. & Bautz, E. K. F. Molec. gen. Genet. 147, 337–341 (1976).
Debenham, P. G. thesis, Univ. Cambridge (1978).
Jorgensen, P. & Fiil, N. P. in Control of Ribosome Synthesis (eds Kjelgaard, N. O. & Maaøe, O.) 370 (Munksgaard, Copenhagen, 1976).
Debenham, P. G. & Travers, A. Eur. J. Biochem. 72, 515–523 (1977).
Gross, C. et al. Proc. natn. Acad. Sci. U.S.A. 75, 427–431 (1978).
Travers, A. A., Kamen, R. I. & Schleif, R. F. Nature 221, 1012–1014 (1970).
Gilbert, W. in RNA polymerase (eds Chamberlin, M. J. & Losick, R.) 193 (Cold Spring Harbor Laboratory, New York, 1976).
Majors, J. Nature 256, 672–674 (1975).
Mitra, S., Zubay, G. & Landy, A. Biochem. biophys. Res. Commun. 67, 857–863 (1975).
Lederberg, E. & Lederberg, J. Genetics 38, 51–64 (1953).
Holland, I. B. & Threlfall, E. J. J. Bact. 97, 91–96 (1969).
Miller, J. H. Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, New York, 1972).
Burgess, R. R. & Travers, A. A. in Proc. Nucleic Acid Research 2 (eds Cantoni, G. L. & Davies, D. R.) 851 (Harper and Row, New York, 1971).
Burgess, R. R. & Jendrisak, J. J. Biochemistry 14, 4634–4638 (1975).
Portalier, R. C., Robert-Baudouy, J. M. & Nemoz, G. M. Molec. gen. Genet. 128, 301–319 (1974).
Nakanishi, S., Adhya, S., Gottesman, M. & Pastan, I. J. biol. Chem. 250, 8202–8208 (1975).
Backman, K., Ptashne, M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 73, 4174–4178 (1976).
Travers, A. Molec. gen. Genet. 147, 225–232 (1976).
Wu, R., Banbara, R. & Jay, E. Crit. Rev. Biochem. 2, 455–512 (1975).
Wechsler, J. A. & Gross, J. D. Molec. gen. Genet. 113, 273–284 (1971).
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Travers, A., Buckland, R., Goman, M. et al. A mutation affecting the σ subunit of RNA polymerase changes transcriptional specificity. Nature 273, 354–358 (1978). https://doi.org/10.1038/273354a0
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DOI: https://doi.org/10.1038/273354a0
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