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GABA of CNS origin in the rat anterior pituitary inhibits prolactin secretion

Nature volume 281, pages 575–578 (1979)Cite this article

Abstract

Hypothalamic hormones synthesised in nuclei within the medial basal hypothalamus (MBH) and extrahypothalamic regions and released from nerve endings at the level of the median eminence (ME) into the hypophysial portal capillary system control anterior pituitary (AP) function1. The median eminence is a key area for neuroendocrine regulation as it receives projections of neurotransmitter pathways which modulate functions such as synthesis and release of hypothalamic hormones2. In addition, transmitters released from the ME into the stalk portal blood may act directly at the AP level to control hormone secretion—this has been demonstrated for dopamine (DA) with respect to prolactin (PRL) secretion3 and it has been suggested that DA itself may represent the so far elusive PRL-inhibiting factor (PIF)2,4,5. Recently, evidence has been presented which suggests that γ-aminobutyric acid (GABA) may also have an important inhibitory role in regulating PRL secretion in the rat. Both GABA and muscimol, a powerful agonist at GABA receptors6, inhibited PRL secretion when injected into urethane anaesthetised female7 or freely moving male rats8. In view of the poor penetrability of the blood-brain barrier (BBB) by both amino acids9 and the recently described specific GABA receptor sites in rat AP10, the above data support the notion that GABA may act directly at the level of the AP to inhibit PRL secretion. This proposition is reinforced by the demonstration that both GABA11 and muscimol12 inhibit PRL secretion from rat isolated APs, an effect which is blocked by the antagonist picrotoxin. We report here that GABA, derived from the central nervous sytem (CNS), is present in the rat anterior pituitary, and we provide evidence that GABA has a functional role in the control of PRL secretion.

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References

  1. Schally, A. V., Coy, D. H. & Meyers, C. A. A. Rev. Biochem. 47, 89–128 (1978).

    Article  CAS  Google Scholar 

  2. Müller, E. E., Nisticò, G. & Scapagnini, U. Neurotransmitters and Anterior Pituitary Function (Academic, New York, 1978).

    Google Scholar 

  3. Ben-Jonathan, N., Oliver, C., Weiner, H. J., Mical, R. S. & Porter, J. C. Endocrinology 100, 452–458 (1977).

    Article  CAS  Google Scholar 

  4. Meites, J. Adv. biochem. Psychopharmac. 16, 138–145 (1977).

    Google Scholar 

  5. Mac Leod, R. M. & Login, I. S. Adv. biochem. Psychopharmac. 16, 147–157 (1977).

    CAS  Google Scholar 

  6. Curtis, D. R., Duggan, A. V., Felix, D. & Johnston, G. A. R. Brain Res. 32, 69–96 (1971).

    Article  CAS  Google Scholar 

  7. Schally, A. V., Redding, T. W., Arimura, A., Dupont, A. & Linthicum, G. L. Endocrinology 100, 681–691 (1977).

    Article  CAS  Google Scholar 

  8. Locatelli, V. et al. Brain Res. 145, 173–179 (1978).

    Article  CAS  Google Scholar 

  9. Enna, S. J., Ferkany, J. W. & Krogsgaard-Larsen, P. in GABA-Neurotransmitters (eds Krogsgaard-Larsen, P., Scheel-Kruger, J. & Kofod, H.) 191–200 (Munksgaard, Copenhagen, 1979).

    Google Scholar 

  10. Grandison, L. & Guidotti, A. Endocrinology 105, 754–759 (1979).

    Article  CAS  Google Scholar 

  11. Enjalbert, A., Ruberg, M., Fiore, L. & Kordon, C. Ann. Endocr. 39, 237–238 (1978).

    CAS  PubMed  Google Scholar 

  12. Locatelli, V. et al. Endocrinology 105, 778–785 (1979).

    Article  CAS  Google Scholar 

  13. Cattabeni, F., Galli, G. & Eros, T. Analyt. Biochem. 72, 1–7 (1976).

    Article  CAS  Google Scholar 

  14. Roberts, E. & Frankel, S. J. biol. Chem. 187, 55–63 (1950).

    CAS  PubMed  Google Scholar 

  15. Pitt, F. N. Jr, Quick, C. & Roberts, E. J. Neurochem. 12, 93–101 (1965).

    Article  Google Scholar 

  16. Saavedra, J. M., Palkovits, M., Kizer, J. S., Brownstein, M. J. & Zivin, J. A. J. Neurochem. 25, 257–260 (1975).

    Article  CAS  Google Scholar 

  17. Fowler, L. J. & John, R. A. Biochem. J. 130, 569–573 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Apud, J. A., Cocchi, D., Müller, E. E. & Racagni, G. in Central and Peripheral Regulation of Prolactin Function (eds Scapagnini, U., Müller, E. E. & MacLeod, R. M.) (Raven, New York, in the press).

  19. Reyntiens, A. J. et al. Arzneit. Forsch. 28, 1194–1196 (1978).

    Google Scholar 

  20. Cocchi, D. et al. Neuroendocrinology (in the press).

  21. Ondo, J. G. & Pass, K. A. Endocrinology 98, 1248–1252 (1976).

    Article  CAS  Google Scholar 

  22. Pass, K. A. & Ondo, J. G. Endocrinology 100, 1437–1442 (1977).

    Article  CAS  Google Scholar 

  23. Mioduszewski, R., Grandison, L. & Meites, J. Proc. Soc. exp. Biol. Med. 151, 44–47 (1977).

    Article  Google Scholar 

  24. Fuxe, K. et al. in GABA-Neurotransmitters (eds Krogsgaard-Larsen, P., Scheel-Kruger, J. & Kofold, H.) 74–94 (Munksgaard, Copenhagen, 1979).

    Google Scholar 

  25. Tappaz, M. L. & Brownstein, M. J. Brain Res. 132, 95–106 (1977).

    Article  CAS  Google Scholar 

  26. Beart, P. M., Kelly, J. S. & Schon, F. Biochem. Soc. Trans 2, 266–268 (1974).

    Article  CAS  Google Scholar 

  27. Holzbauer, M., Sharman, D. F. & Godden, U. Neuroscience 3, 1251–1262 (1978).

    Article  CAS  Google Scholar 

  28. Cattabeni, F., Maggi, A., Monduzzi, M., De Angelis, L. & Racagni, G. J. Neurochem. 31, 565–567 (1978).

    Article  CAS  Google Scholar 

  29. Guidotti, A. et al. Neuropharmacology 13, 1115–1122 (1974).

    Article  CAS  Google Scholar 

  30. Glowinski, J. & Iversen, L. L. J. Neurochem. 13, 655–669 (1966).

    Article  CAS  Google Scholar 

  31. Cattabeni, F., Koslow, S. H. & Costa, E. Science 178, 166–167 (1972).

    Article  ADS  CAS  Google Scholar 

  32. Roberts, E. & Simonsen, D. G. Biochem. Pharmac. 12, 113–116 (1963).

    Article  CAS  Google Scholar 

  33. Macaione, S. J. Neurochem. 19, 1397–1400 (1972).

    Article  CAS  Google Scholar 

  34. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).

    CAS  Google Scholar 

  35. Altaffer, F. B., de Balbian Vester, F., Hall, S., Long, C. J. & D'Encarnacao, P. Physiol. Behav. 5, 119–121 (1970).

    Article  CAS  Google Scholar 

  36. Falconi, G. & Rossi, G. L. Endocrinology 74, 301–303 (1964).

    Article  CAS  Google Scholar 

  37. Rerup, C. & Lundquist, I. Acta endocr. 52, 357–367 (1966).

    CAS  PubMed  Google Scholar 

  38. Niswender, G. D., Chen, C. L., Midgley, A. R. Jr, Meites, J. & Ellis, S. Proc Soc. exp. Biol. Med. 130, 793–797 (1969).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Pharmacology and Pharmacognosy, University of Milan, Italy

    G. Racagni & J. A. Apud

  2. Department of Pharmacology, University of Milan, Italy

    V. Locatelli & D. Cocchi

  3. Institutes of Pharmacology and Biochemistry, University of Messina, Italy

    G. Nistico' & R. M. di Giorgio

  4. Institute of Pharmacology and Pharmacognosy, University of Cagliari, Italy

    E. E. Müller

Authors
  1. G. Racagni
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  2. J. A. Apud
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  3. V. Locatelli
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  4. D. Cocchi
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  5. G. Nistico'
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  6. R. M. di Giorgio
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  7. E. E. Müller
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Racagni, G., Apud, J., Locatelli, V. et al. GABA of CNS origin in the rat anterior pituitary inhibits prolactin secretion. Nature 281, 575–578 (1979). https://doi.org/10.1038/281575a0

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