Abstract
IT has recently been suggested by Roseman et al.1 that columns of ‘Amberlite IRA 400 (OH′)’ might be used for the separation of certain reducing and non-reducing carbohydrates. Our own experience with this resin suggests that very carefully controlled conditions would be necessary if reliable quantitative results are to be obtained. During an investigation of the non-volatile organic acid components of apple juice and cider, it has been found that sugars are extensively degraded by ‘Amberlite IRA 400’ used in the OH′ form, organic acids being the main products of degradation.
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References
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PHILLIPS, J., POLLARD, A. Degradation of Sugars on Ion-Exchange Columns of ‘Amberlite IRA 400 (OH′)’. Nature 171, 41–42 (1953). https://doi.org/10.1038/171041b0
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DOI: https://doi.org/10.1038/171041b0
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