Abstract
A PREVIOUS publication from this laboratory1 has reported in detail the rapid oxidation of glycerol to dihydroxyacetone by resting whole cells of A. suboxydans 621, at pH 6.0. This conversion proceeds more slowly beyond the dihydroxyacetone stage. In cell-free extracts with triphenyltetrazolium chloride as the electron acceptor, glycerol is oxidized at a rate comparable to the oxidation of dihydroxyacetone, with the pH optimum about 8.5. This contrasting behaviour has been found to be due to the presence of two major oxidative routes for glycerol oxidation, one phosphorylative, the other independent of phosphate, as shown in Table 1. With cell-free extracts at pH 8.5, adenosine triphosphate is obligatory (in kinase systems) not only for the oxidation of glycerol but also for dihydroxyacetone, glucose, ribose or erythritol.
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References
King, T. E., and Cheldelin, V. H., J. Bact., 66, 581 (1953).
Horecker, B. L., Brewers Digest, 28, 214 (1953).
Hauge, J. G., King, T. E., and Cheldelin, V. H., Fed. Proc., 13, 225 (1954).
Roe, J. H., J. Biol. Chem., 107, 15 (1934).
King, T. E., and Cheldelin, V. H., J. Biol. Chem., 198, 127 (1952).
Horecker, B. L., Gibbs, M., Klenow, H., and Smyrniotis, P. Z., J. Biol. Chem., 207, 393 (1954).
Axelrod, B., Bandurski, R. S., Greiner, C. M., and Jang, R., J. Biol. Chem., 202, 619 (1953). Gibbs, M., and Horecker, B. L., J. Biol. Chem., 208, 813 (1954).
Racker, E., de la Haba, G., and Leder, I. G., J. Amer. Chem. Soc., 75, 1010 (1953).
Lanning, M. C., and Cohen, S. S., J. Biol. Chem., 207, 193 (1954).
Newburgh, R. W., and Cheldelin, V. H. (unpublished observations).
King, T. E., and Cheldelin, V. H., Biochim. Biophys. Acta, 14, 108 (1954).
Mejbaum, W., Z. physiol. Chem., 258, 117 (1939).
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HAUGE, J., KING, T. & CHELDELIN, V. Alternate Pathways of Glycerol Oxidation in Acetobacter suboxydans . Nature 174, 1104–1105 (1954). https://doi.org/10.1038/1741104a0
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DOI: https://doi.org/10.1038/1741104a0
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