Abstract
BEMEGRIDE (β-ethyl β-methyl glutarimide), labelled with carbon-14 in both the α positions, is excreted largely in the urine and to a lesser extent in the bile and fæces when administered to mice, rats and guinea pigs1. Chromatograms of urine and bile from these animals were developed by the ascending technique using n-butanol/acetic acid/water (12: 3: 5, v/v). Autoradiographs of chromatograms of guinea pig bile showed only one spot (R F 0.88) which was identical in mobility to the control-labelled bemegride. The urine of rats, collected 15–120 min. after administration of labelled bemegride, revealed two spots of R F values 0.88–0.89 and 0.77–0.81. The faster moving fraction moved as one spot when labelled bemegride was added to the urine before chromatography. The spot with the lower mobility increased in intensity over the 15–120 min. period of urine collection. Urine from mice, 15–120 min. after bemegride injection, showed two spots with R F values similar to those of rat urine, while urine collected 8 hr. after injection revealed a third spot of R F 0.40–0.46. This spot became progressively more intense and the faster moving fractions correspondingly less up to 24 hr.
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NICHOLLS, P. Metabolism of Bemegride labelled with Carbon-14. Nature 185, 927 (1960). https://doi.org/10.1038/185927a0
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DOI: https://doi.org/10.1038/185927a0
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