Abstract
A PREVIOUS communication1 reported the isolation of a B-active disaccharide, identified as 3-O-α-D-galacto-pyranosyl-D-galactoso, from the products of partial acid-hydrolysis of a human blood-group B specific mucopolysaccharide. This sugar was the only disaccharide detected that showed B-activity, and its absence from the hydrolysis products of human blood-group A specific substance2,3, together with other evidence, led us to infer that this disaccharide is the terminal non-reducing unit of the blood-group B determinant structure in the original mucopolysaccharide. However, the capacity of the disaccharide to inhibit the agglutination of B erythrocytes by a human anti-B serum, although much greater than that of any simple sugar previously examined, was nevertheless much lower than that of the intact B-active mucopolysaccharide in the same system, thus supporting the idea1,4 that the complete B-determinant structure is larger than a disaccharide unit. To investigate this possibility, the trisaccharide components in the hydrolysis products were examined, and the isolation and identification of two B-active trisaccharides are now described.
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PAINTER, T., WATKINS, W. & MORGAN, W. Isolation of Two Serologically Active Trisaccharides from Human Blood-group B Substance. Nature 199, 282–283 (1963). https://doi.org/10.1038/199282a0
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DOI: https://doi.org/10.1038/199282a0
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