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A radiation hybrid map of 506 STS markers spanning human chromosome 11

Nature Genetics volume 8, pages 70–76 (1994)Cite this article

Abstract

We present a high resolution radiation hybrid map of human chromosome 11 using 506 sequence tagged sites (STSs) scored on a panel of 86 radiation hybrids. The 506 STSs fall into 299 unique positions (average resolution of about 480 kilobases (kb)) that span the whole chromosome. A subset of 260 STSs (143 positions) form a framework map that has a resolution of approximately 1 megabase between adjacent positions and is ordered with odds of at least 1,000:1. The centromere was clearly defined with pericentric markers unambiguously assigned to the short or long arm. The map contains most genes (125) and expressed sequence tags (26) currently assigned to chromosome 11 and more than half of the STSs are polymorphic microsatellite loci. These markers and the map can be used for high resolution physical and genetic mapping.

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Author notes

  1. M.R. James

    Present address: The Wellcome Trust Centre for Human Genetics, Nuffield Orthopaedic Centre, Windmill Road, Headington, Oxford, OX3 7LD, UK

Authors and Affiliations

  1. INSERM U358,

    M.R. James, J.-J. Schott, C. Yousry, C. Dubay, M. Agrapart & G.M. Lathrop

  2. Centre d'Etude du Polymorphisme Humain, 27 rue Juliette Dodu, Paris, France

    M.R. James, J.-J. Schott, C. Yousry, C. Dubay, M. Agrapart & G.M. Lathrop

  3. Departments of Psychiatry and Human Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, 15213, USA

    C.W. Richard III

  4. Institute of Molecular Medicine, John Radcttffe Hospital, Oxford, OX39DU, UK

    K. Clark & J. Bell

  5. Department of Genetics and Development, Columbia University, New York, New York, 10032, USA

    J.D. Terwilliger

  6. CNRS URA 1445, Institut Pasteur, Paris, France

    J. Kazan & J. Weissenbach

  7. Généthon, 1 rue de l'Intemationale, 91000, Evry, France

    A. Vignal & J. Weissenbach

  8. Department of Biochemistry, Cancer Institute, Kami-Ikebukuro, Tokyo, 170, Japan

    T. Imai & Y. Nakamura

  9. National Center for Human Genome Research, National Institutes of Health, Building 49, Bethesda, Maryland, 20892, USA, 10

    M. Polymeropoulos

  10. Department of Genetics, Stanford University, Stanford, California, 94305, USA

    D.R. Cox

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James, M., Richard, C., Schott, JJ. et al. A radiation hybrid map of 506 STS markers spanning human chromosome 11. Nat Genet 8, 70–76 (1994). https://doi.org/10.1038/ng0994-70

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