Microelectrode studies on Malpighian tubule cells of Locusta: Effects of cyclic AMP, IBMX and corpora cardiaca extract

doi:10.1016/0022-1910(89)90112-1

Journal of Insect Physiology

Volume 35, Issue 5, 1989, Pages 387-392

https://doi.org/10.1016/0022-1910(89)90112-1 Get rights and content

Abstract

Basal, apical and transepithelial potentials were recorded from Malpighian tubules of Locusta with a view to establishing the endocrine control of ion movements across this epithelium. Treatment with corpora cardiaca extract with established diuretic activity resulted in depolarization of the potential across the apical plasma membrane (V_a) and a small hyperpolarization of basal cell membrane potential (V_b). In the absence of Cl⁻, however, corpora cardiaca extract effected a depolarization of V_b and a hyperpolarization of V_a. Similarly, treatment with either IBMX or dibutyryl cyclic AMP effected a hyperpolarization of V_a in control saline and this was further increased by the latter under Cl⁻-free conditions. High [K⁺], Na⁺-free salines produced a depolarization of V_a and V_b; the subsequent addition of corpora cardiaca extract effecting a further marked depolarization of V_a. The results suggest that some factor(s) present in the corpora cardiaca stimulate cation and anion movements across the tubule wall and that this action is mediated by more than one second messenger.

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Rubidium reduces potassium permeability and fluid secretion in Malpighian tubules of Locusta migratoria, L
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The basal membrane potential (V_b) of Locusta Malpighian tubule cells in control saline results from its relatively high permeability to potassium. In the presence of 1 mM barium added to the control saline V_b hyperpolarized from a mean resting potential of −72.1 mV to −90.1 mV. On substituting rubidium for potassium in the control saline, V_b also hyperpolarized to a value of −91.4 mV. Rubidium was also similarly effective in hyperpolarizing the basal membrane even in the presence of control concentrations of potassium in the bathing medium. Substitution of rubidium for potassium also effected a ∼50% reduction in the rate of fluid secretion. The action of inhibitors on V_b in the presence of rubidium showed that V_b under these conditions probably originated from the bafilomycin-sensitive electrogenic potential generated across the apical membrane by a V-type ATPase. The responses of V_b to potassium, barium and rubidium and their inhibition of fluid secretion suggest the presence of a substantial rubidium-blockable potassium conductance located on the basal membrane of Locusta Malpighian tubule cells.
The Drosophila melanogaster malpighian tubule
2001, Advances in Insect Physiology
This chapter reviews the morphology, function, and development of malpighian tubule of Drosophila melanogaster. Recent results have provided a reference point for tubule studies for insect physiologists and a unique resource for developmental and genetic analysis of fundamental questions of differentiated cell function. It is noted that the formation of the Drosophila melanogaster malpighian tubule is better understood than that of any other insect and it provides a model for epithelial development in general. In addition to the standard Drosophila techniques of close microscopic examination, genetic screens, enhancer trapping, and reporter gene expression, there are some specific tools that have made progress much easier.
The action of inhibitors of protein kinases on fluid and ion secretion by Malpighian tubules of Locusta migratoria, L.
1998, Journal of Insect Physiology
Fluid production in Locusta Malpighian tubules was stimulated by corpora cardiaca extract (c. 100%) and dibutyryl cAMP (c. 50%). Chelerythrine and staurosporine (Protein kinase C, PKC inhibitors) inhibited it in the range 0.07–60 μM (IC₅₀ 3 μM), whereas Rp-cAMP (Protein kinase A, PKA inhibitor) caused inhibition over the concentration range 10–1000 μM (IC₅₀ 264 μM). The protein phosphatase inhibitor, okadaic acid, was also inhibitory over the concentration range 0.1–1000 nM (IC₅₀ 91 nM). CC extract stimulation increased fluid [Na⁺] from 41 to 59 mM and decreased [K⁺] from 127 to 107 mM; stimulation with cAMP had no such effect. The PKC inhibitors reduced the [K⁺] in the secreted fluid from 126 to 107 mM but had no effect on the [Na⁺]. Subsequent addition of CC extract stimulated fluid production and caused an increase in [Na⁺] from 41 to about 50 mM. The addition of Rp-cAMP reduced fluid production but caused a decrease in [Na⁺] from 37 to 28 mM and an increase in its [K⁺] from 124 to 148 mM. Fluid production by Rp-cAMP inhibited tubules was not stimulated by corpora cardiaca extract or cAMP, but [Na⁺] rose to 36 mM. Protein phosphorylation plays a role in the regulation of fluid production probably via the apical and basal membrane cation transporters.
The isolation and identification of three diuretic kinins from the abdominal ventral nerve cord of adult Helicoverpa zea
1995, Journal of Insect Physiology
Three novel diuretic peptides have been isolated and identified from the abdominal ventral nerve cord of the adult corn earworm, Helicoverpa zea. The peptides belong to the kinin family of myotropic and diuretic peptides and have the following sequences: YFSPWGamide, VRFSPWGamide, and KVKFSAWGamide. These peptides, which we refer to as the helicokinins, are potent stimulators of secretion by the Malpighian tubules of Manduca sexta. All three of the helicokinins stimulated secretion by M. sexta tubules at concentrations below 10⁻¹¹ M. The helicokinins are the first kinins to be identified from a lepidopteran, and the first diuretic peptides to be identified specifically from the abdominal ventral nerve cord of any insect.
Effects of anions, acetazolamide, thiocyanate and amiloride on fluid secretion by the Malpighian tubules of Locusta migratoria L.
1994, Journal of Insect Physiology
The effect of Cl⁻, HCO₃⁻, Br⁻, acetazolamide, thiocyanate and amiloride on urine formation in Locusta migratoria Malpighian tubules have been determined. The rate of fluid secretion depends markedly on the concentration of Cl⁻ in the bathing solution with concentrations less than 90 mM resulting in reduced fluid secretion. Substitution of Br⁻ for Cl⁻ had no significant effect on the rate of the fluid secretion. Replacement of NaHCO₃ with NaCl in Hepes buffered Ringer solution reduced the rate of urine production by 23%. Fluid secretions were reduced in the presence of 10⁻⁴–10⁻² M acetazolamide, a carbonic anhydrase inhibitor. The combined effect of acetazolamide in the absence of HCO₃⁻ appears to be additive. A 1 mM concentration of thiocyanate, an ionic inhibitor, reduced fluid secretion by 35%. Amiloride interferes with the electrogenic entry of Na⁺ into the cell and a 1 mM solution reduced fluid secretion by 94% with secretion completely inhibited in 80% of the tubules tested.
The ionic basis of fluid secretion in insect Malpighian tubules: Advances in the last ten years
1993, Journal of Insect Physiology
Current information on the ionic basis of fluid secretion in Malpighian tubules is reviewed, with the emphasis on electrophysiological data, almost all of which has appeared in the last decade. Different electrical techniques are compared. Evidence for the various channels, carriers and pumps thought to be involved in the transport of K, Na, H and Cl ions is discussed. Few insect species have been studied in any detail, and major differences between them are apparent. Rapid advances are anticipated when synthetic diuretic peptides become available for electrophysiological studies.

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Journal of Insect Physiology

Abstract

References (29)

Fluid and cation secretion by Malpighian tubules of Locusta

J. Insect Physiol.

Some factors affecting Malpighian tubule fluid secretion and trans-epithelial potential in Locusta migratoria L.

Experientia

The role of cyclic AMP in relation to the diuretic hormone of Rhodnius prolixus

J. Insect Physiol.

Microelectrode studies on Malpighian tubule cells of Locusta migratoria: effects of external ions and inhibitors

J. Insect Physiol.

Membrane permeability changes during stimulation of isolated salivary glands of Calliphora by 5-hydroxytryptamine

J. Physiol.

Studies on the mechanism of fluid secretion by isolated salivary glands of Calliphora

J. exp. Biol.

Transepithelial potential changes during stimulation of isolated salivary glands with 5-hydroxytryptamine and cyclic AMP

J. exp. Biol.

Effect of Na⁺, K⁺, ouabain, amiloride and ethacrynic acid on the transepithelial potential across Malpighian tubules of Locusta

J. Insect Physiol.

Distributions of ions in a fluid-transporting epithelium determined by electron-probe X-ray microanalysis

Nature, Lond.

The mechanism of insect excretory systems

Adv. Insect Physiol.

Characteristics of epithelial transport in insect Malpighian tubules

Hormonal control of Malpighian tubule and rectal function in the desert locust Schistocerca gregaria

J. Insect Physiol.

The physiological effects of corpus cardiacum extracts in locusts

Gen. comp. Endocr.

Cited by (15)

Rubidium reduces potassium permeability and fluid secretion in Malpighian tubules of Locusta migratoria, L

The Drosophila melanogaster malpighian tubule

The action of inhibitors of protein kinases on fluid and ion secretion by Malpighian tubules of Locusta migratoria, L.

The isolation and identification of three diuretic kinins from the abdominal ventral nerve cord of adult Helicoverpa zea

Effects of anions, acetazolamide, thiocyanate and amiloride on fluid secretion by the Malpighian tubules of Locusta migratoria L.

The ionic basis of fluid secretion in insect Malpighian tubules: Advances in the last ten years

Microelectrode studies on Malpighian tubule cells of Locusta: Effects of cyclic AMP, IBMX and corpora cardiaca extract

Abstract

Fluid and cation secretion by Malpighian tubules of Locusta

J. Insect Physiol.

Some factors affecting Malpighian tubule fluid secretion and trans-epithelial potential in Locusta migratoria L.

Experientia

The role of cyclic AMP in relation to the diuretic hormone of Rhodnius prolixus

J. Insect Physiol.

Microelectrode studies on Malpighian tubule cells of Locusta migratoria: effects of external ions and inhibitors

J. Insect Physiol.

Membrane permeability changes during stimulation of isolated salivary glands of Calliphora by 5-hydroxytryptamine

J. Physiol.

Studies on the mechanism of fluid secretion by isolated salivary glands of Calliphora

J. exp. Biol.

Transepithelial potential changes during stimulation of isolated salivary glands with 5-hydroxytryptamine and cyclic AMP

J. exp. Biol.

Effect of Na+, K+, ouabain, amiloride and ethacrynic acid on the transepithelial potential across Malpighian tubules of Locusta

J. Insect Physiol.

Distributions of ions in a fluid-transporting epithelium determined by electron-probe X-ray microanalysis

Nature, Lond.

The mechanism of insect excretory systems

Adv. Insect Physiol.

Characteristics of epithelial transport in insect Malpighian tubules

Hormonal control of Malpighian tubule and rectal function in the desert locust Schistocerca gregaria

J. Insect Physiol.

The physiological effects of corpus cardiacum extracts in locusts

Gen. comp. Endocr.

Effect of Na⁺, K⁺, ouabain, amiloride and ethacrynic acid on the transepithelial potential across Malpighian tubules of Locusta