Determination of free salsolinol concentrations in human urine using gas chromatography—mass spectrometry

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Abstract

The urine concentrations of free salsolinol were determined in six healthy volunteers, using a gas chromatographic—mass spectrometric method with electron-capture negative-ion chemical ionization after derivatization with pentafluoropropionyl anhydride. The sensitivity of this method allows the quantification of salsolinol concentrations of 0.55 pmol/ml. The synthesis of [2H4]salsolinol from dopamine and [2H4]acetaldehyde via a Pictet—Spengler condensation is described; [2H4]salsolinol was used as the internal standard for salsolinol quantification. The urine concentrations of free salsolinol ranged from ca. 1 to 6 pmol/ml.

References (17)

  • B. Sjöquist et al.

    J. Chromatogr.

    (1980)
  • J. Odink et al.

    J. Chromatogr.

    (1986)
  • E. Pianezzola et al.

    J. Chromatogr.

    (1989)
  • M.A. Collins et al.

    Prog. Clin. Biol. Res.

    (1982)
  • P. Dostert et al.

    J. Neural Transm.

    (1988)
  • R.D. Myers

    Experientia

    (1989)
  • M.A. Collins et al.

    Science

    (1979)
  • B. Sjöquist et al.

    Subst. Alc. Actions Misuse

    (1981)
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